MD10G1268400.v1.1

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001944
Physical Location & Seq
Reverse (-)
36111145 .. 36118637
7493 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1268400.v1.1.491

Sequence Viewer

Length: 3837 bp
ATGGAAGGAGGAAGAGGAGGAGGGGAAGGCAACGCAGAAGTTAATAATAATAATGGGTCACAAGAGAAAGCAGATAAAAAAAAGCAGAAAGTCGCCTTCTATAAGCTCTTCTCGTTTGCAGACCGTTTGGACGTTGCGTTGATGATCGTTGGAACGATATCTGCGGTGGGGAATGGACTGTCGAAGCCGCTCATGACTCTTGTGTTTGGTAATCTTATTAACACCTTTGGTTGCACCGATCCTGGTCACATCGTTCCCATGATATCTAAGGTGTCTCTAAAGTTTGTGTACTTGGCCATTGGCACTGGAGCTGCAGCACTTCTACAGGTGGCATGTTGGATGGTAACAGGAGAAAGGCAGGCTGCTAGAATTAGAGGCAAGTACATGAAGGCAATCCTAAGGCAGGACATTGGCTTCTTTGACACTGAAACTAGCACTGGAGAGATCATTGGCAGGATGTCTGGGGACACCATTCTTATTCAAGAAGCCATGGGGGAAATGGTGGGGAAGTTTATACAACTTTGTTCTACTTTTCTTGGTGGGTTTGTGATTGCTTTTGTCAAGGGGTGGCGGCTCACTGTTGTATTGTTGGCCACCATTCCTGCCATTGTCATTGCTGGGGCAGTAATGGCTACTATCATGTCGAGAATGTCGTCCCGCGGACAGTCTGCTTATGCAGAAGCTGGCAGTATAGTAGAACAAACAGTTGGTTCCATTAGAACAGTTGCATCTTTCACTGGGGAGAAGCAAGCAATTGAACAGTACAACCAGAAGCTAAAAGTTGCTTACAATACTATGGTTCAACAAGGGTTAGTCACAGGGATTGGACTCGGAATATTTATGCTTGTCATCTTCTGCTCATACGCGCTTGCGGTATGGTATGGATCCAAAATGATCATCAAACATGGATATAATGGAGGCCAAGTTATCAATGTTATATTTGCACTCATGACTGGTGGAATGTCACTTGGTCAGGCATCACCGTCTTTGAATGCATTCGCATCAGGGAAGGCTGCTGCATATAAAATGTTTGAGACCATCAGCCGAAATCCAACTATCGATCCCTACGATACAAGTGGAATTGTATTGCAAGATGTTAAAGGTGATGTCGAACTCAAAGACGTGTATTTCAGGTACCCTGCCAGACCAGACGTGCAGATCTTTGCTGAGTTCTCATTATATGTTCCAAGTGGCACAACCACTGCCTTGGTTGGGCAGAGTGGAAGCGGGAAGTCGACAGTGATCGGGCTAGTAGAAAGGTTTTATGATCCAGAAGCCGGTGAAGTACTTATAGATGGCGTGGACTTGAAGAAGTTGCAGCTTAAAGCGATAAGGGAGAAGATCGGGCTGGTTAGTCAGGAACCGAATCTCTTTACAACAACTATAAGGGAGAACATAAAATATGGGAAGGATGATGCAACTGATGAGGAGATTAGAAAAGCAACTGAGCTCGCAAATGCAGCAAAATTCATTGATAAGCTACCCCAGGGGATCGACACAATGGTAGGTGAGCATGGGACATCTCTATCTGGTGGACAAAAGCAGAGAATTGCAATTGCAAGAGCCATTCTGAAGAACCCGAGAATTCTTCTTCTTGATGAAGCAACAAGCGCTCTGGATGCTGAGTCCGAAAAGATTGTTCAAGATGCACTGGTGAACTTGATGTCAAATCGCACAACCATAGTTGTTGCGCATCGCTTAACAACTGTTAGGAATGCTGACTGCATTGCAGTGGTGCACAGAGGAAAAATTGTGGAAAAAGGGACTCATGATGAGTTGACCAGAGATCCGGAAGGGGCTTATAGCCAACTAATCCACCTGCAAGAAGGATCGAAAGTGGACAACGGAGCACAAACCTCTGATCCGAATAGAATGGATACCAGCTTAGATATAGATAGGACTATGCTCAGCTCTGGGAGCCGGAGATTATCAATGAGAAGGTCTATAAGCAGAGGCTCATCAGGTGGCCGGCACTCATTCACTGTTGGCTTTGGCATTCCTGGTCCAATTGATGTCCAAGAGAATGAAGTAGGACACGAGGACGATCATGACAGACCTAAGGTTGATTCCAAAAAGCGCAACACAGTTTCAATCCGAAGGCTGGCCGCCCTTAACAAACCCGAGGTTCCAGTTTTGCTTCTAGGAGCCATTGCTGCAGCAGGGCATGGAGTACTTTTCCCTATCTTTGGCTTACTACTCTCAAAAGCCATTGGAATGTTCTACGAACCGCCCAATGAGCTAAGGCACGATTCTAGAAAATGGGCATTAGTTTATGTAGGTTTAGGTTGTGCTGGTCTGTTGGTGGTTCCTGTGCAGAATTTCTTCTTTGGAGTTGCAGGTGGGAAATTGGTGGAGCGAATTCGTGCCTTGTCATTCCAGAAGGTTGTGCACCAGCAAGTCAGTTGGTTTGATGATCCTGCAAATTCAAGCGGTGCCATTGGCGCAAGGTTATCTACGGATGCTTCAACCATTAAGAGTCTTGTTGGTGACGCCTTGGCGTTGATTGTGCAGAATATAGCAACAATCATGGCAGGGCTGGTTATAGGTTTCACAGCTAACTGGAAACTGATGCTGGTAGTTTTAGCCGTTTCGCCATTGATTCTTCTGCAAGGAACACTTCAGGCAAAATTTCTTAAAGGATTTAGTGCAGATGCCAAGATTATGTACGAAGAAGCCAGTCAAGTGGCAAACGATGCGATTGGTAGCATCCGAACGGTTGCATCATTTTGTTCTGAGAAGAAGGTGATGGAGGCATACGAGAAGAAGTGTGAGGGTCCGTTGAAGCAAGGAGTACGGTTAGGAGTAGTAAGTGGTTCAGGTTTTGGCTTTTCATTCTTCTTAATGTTCTGCACAAATGCGTTGATATTCTATATTGGAGCTATTCTTGTGAAACATGGTCAAGCTACATTTGAACAAGTATTCAAGGTTTTCTTTGCTATAACAATCACTGCAATGGGGGTCTCACAAGCTACTAGCATGGCTCCTGACTCCAACAAAGCCAAGGACTCAGCAGCTTCGATATTTCGAATTCTCGACAGCAAGCCTAAGATTGACTCGAGCAGTGATGAAGGCATAGCGTTATCACTTTTAATTGGAGAAATTGAGCTTGATCATGTCAGCTTTAAGTATCCTACACGCCCGGATGTGCAGATATTCAGAGACATTTGTTTGAAGATGCCCTCCGGAAAGACTGTTGCTTTGGTTGGAGAGAGTGGCAGCGGGAAGTCAACGGTAATAGGCCTCATAGAGAGATTTTACGATCCTGATTCAGGTCGCGTACTACTAGACGGAGTAGAAATCCAAAAGTTCAAGCTAAACTGGCTGAGGCAGCAGATAGGGTTGGTTGGTCAAGAACCAGTTCTCTTCAACGAATCCATTCGTGCCAACATTGCATACGGCAAGCCAGGAGATGTTACCGAGGAAGAGATCATAGCAGCGACAACAGCAGCTAACGTGCACAATGTCATATCTTCATTGCCTCAAGGCTACGACGCCTCAGTAGGTGAACGAGGGGTTCAGCTATCAGGAGGTCAGAAGCAAAGAATCGCCATTGCGAGGGCTATACTCAAGGACCCAAAAATCCTTCTACTTGATGAAGCTACAAGTGCACTGGATGCAGAGTCCGAACGCAAAGTGCAAGACGCATTGGACAGGGTTATGGTGAACAGAACAACAGTTGTAGTTGCGCACCGCCTTAGCACGATAAAAGGAGCAGACATAATTGCAGTGGTGAAGAATGGCGTGATTGCGGAGAAGGGAAGCCATGATTTCCTAATGAAGATCACTGACGGGGCTTATGCTTCTTTGGTGGCACTTCATTCCAGCTCAAACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1279

Amino Acids

137.58

Weight (kDa)

8.62

Isoelectric Point (pI)

31.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 48 - 319 1.3e-62 ABC transporter transmembrane region
ABC_tran PF00005 390 - 537 1.9e-33 ABC transporter
SMC_N PF02463 426 - 579 1e-05 RecF/RecN/SMC N terminal domain
ABC_membrane PF00664 712 - 985 5.4e-60 ABC transporter transmembrane region
ABC_tran PF00005 1053 - 1202 1.6e-32 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1827, 2327
Acc16I TGCGCA 2 cut(s) 1692, 3687
Acc36I ACCTGC 2 cut(s) 1827, 2327
Acc65I GGTACC 1 cut(s) 1134
AccB1I GGYRCC 2 cut(s) 1134, 2432
AccBSI CCGCTC 1 cut(s) 190
AccI GTMKAC 1 cut(s) 1235
AccII CGCG 3 cut(s) 660, 866, 3276
AccIII TCCGGA 2 cut(s) 1789, 3182
AcoI YGGCCR 4 cut(s) 294, 591, 1966, 2103
AcsI RAATTY 7 cut(s) 1466, 1584, 2317, 2358, 2422, 2627, 3027
AcuI CTGAAG 2 cut(s) 1592, 2603
AcyI GRCGYC 2 cut(s) 2490, 3492
AfaI GTAC 9 cut(s) 290, 383, 764, 1136, 1287, 2172, 2666, 2793, 3279
AfeI AGCGCT 1 cut(s) 1612
AfiI CCNNNNNNNGG 7 cut(s) 403, 1212, 1277, 2101, 2186, 3269, 3555
AflIII ACRYGT 1 cut(s) 1122
AjiI CACGTC 2 cut(s) 1123, 1153
AjnI CCWGG 4 cut(s) 241, 1485, 1999, 3403
AjuI GAANNNNNNNTTGG 2 cut(s) 690, 722
Alw21I GWGCWC 6 cut(s) 1452, 1740, 1852, 2391, 3459, 3610
Alw26I GTCTC 4 cut(s) 279, 1028, 2965, 3153
Alw44I GTGCAC 4 cut(s) 1736, 2387, 3455, 3606
AlwNI CAGNNNCTG 1 cut(s) 683
Ama87I CYCGRG 3 cut(s) 1579, 2120, 3055
Aor13HI TCCGGA 2 cut(s) 1789, 3182
Aor51HI AGCGCT 1 cut(s) 1612
AoxI GGCC 6 cut(s) 294, 591, 919, 1966, 2103, 3238
ApaLI GTGCAC 4 cut(s) 1736, 2387, 3455, 3606
ApoI RAATTY 7 cut(s) 1466, 1584, 2317, 2358, 2422, 2627, 3027
ArsI GACNNNNNNTTYG 2 cut(s) 3181, 3213
Asp700I GAANNNNTTC 4 cut(s) 995, 2321, 3357, 3375
Asp718I GGTACC 1 cut(s) 1134
AspLEI GCGC 6 cut(s) 868, 1613, 1693, 2079, 2444, 3688
AspS9I GGNCC 3 cut(s) 2003, 2774, 3571
AsuC2I CCSGG 1 cut(s) 3140
AsuII TTCGAA 1 cut(s) 3025
AvaI CYCGRG 3 cut(s) 1579, 2120, 3055
AvaII GGWCC 3 cut(s) 2003, 2774, 3571
AxyI CCTNAGG 2 cut(s) 398, 2058
BaeGI GKGCMC 4 cut(s) 1740, 2391, 3459, 3610
BalI TGGCCA 2 cut(s) 296, 593
BamHI GGATCC 1 cut(s) 884
BanI GGYRCC 2 cut(s) 1134, 2432
BanII GRGCYC 1 cut(s) 1452
BauI CACGAG 1 cut(s) 2036
Bbv12I GWGCWC 6 cut(s) 1452, 1740, 1852, 2391, 3459, 3610
BbvCI CCTCAGC 1 cut(s) 3323
BccI CCATC 4 cut(s) 334, 1046, 1289, 2740
BceAI ACGGC 2 cut(s) 2570, 3412
BciT130I CCWGG 4 cut(s) 243, 1487, 2001, 3405
BciVI GTATCC 2 cut(s) 1870, 3138
BclI TGATCA 2 cut(s) 894, 3109
BcnI CCSGG 1 cut(s) 3140
BcoDI GTCTC 4 cut(s) 279, 1028, 2965, 3153
BfaI CTAG 7 cut(s) 366, 432, 1250, 2141, 2253, 2973, 3284
BfmI CTRYAG 3 cut(s) 312, 323, 2154
BfoI RGCGCY 1 cut(s) 1614
BfuAI ACCTGC 2 cut(s) 1827, 2327
BfuI GTATCC 2 cut(s) 1870, 3138
BglII AGATCT 1 cut(s) 1158
BlpI GCTNAGC 1 cut(s) 1907
BmcAI AGTACT 2 cut(s) 1287, 2172
Bme1390I CCNGG 5 cut(s) 243, 1487, 2001, 3140, 3405
Bme18I GGWCC 3 cut(s) 2003, 2774, 3571
BmeT110I CYCGRG 3 cut(s) 1579, 2120, 3055
BmgBI CACGTC 2 cut(s) 1123, 1153
BmgT120I GGNCC 3 cut(s) 2003, 2774, 3571
BmrFI CCNGG 5 cut(s) 243, 1487, 2001, 3140, 3405
BmrI ACTGGG 1 cut(s) 747
BmuI ACTGGG 1 cut(s) 747
BpmI CTGGAG 2 cut(s) 327, 459
Bpu10I CCTNAGC 3 cut(s) 2240, 3323, 3695
Bpu1102I GCTNAGC 1 cut(s) 1907
Bpu14I TTCGAA 1 cut(s) 3025
BpuEI CTTGAG 2 cut(s) 3465, 3551
BpuMI CCSGG 1 cut(s) 3140
Bsa29I ATCGAT 1 cut(s) 1059
BsaHI GRCGYC 2 cut(s) 2490, 3492
BsaI GGTCTC 2 cut(s) 1028, 2965
BsaJI CCNNGG 9 cut(s) 489, 658, 1206, 1485, 1486, 2121, 2493, 3000, 3417
BsaWI WCCGGW 2 cut(s) 1789, 3182
BsaXI ACNNNNNCTCC 2 cut(s) 909, 939
Bsc4I CCNNNNNNNGG 7 cut(s) 403, 1212, 1277, 2101, 2186, 3269, 3555
Bse118I RCCGGY 2 cut(s) 1277, 1968
Bse21I CCTNAGG 2 cut(s) 398, 2058
Bse3DI GCAATG 7 cut(s) 612, 1725, 2148, 2958, 3387, 3473, 3549
BseAI TCCGGA 2 cut(s) 1789, 3182
BseBI CCWGG 4 cut(s) 243, 1487, 2001, 3405
BseCI ATCGAT 1 cut(s) 1059
BseDI CCNNGG 9 cut(s) 489, 658, 1206, 1485, 1486, 2121, 2493, 3000, 3417
BseGI GGATG 8 cut(s) 345, 462, 1417, 1624, 2464, 2706, 3148, 3619
BseLI CCNNNNNNNGG 7 cut(s) 403, 1212, 1277, 2101, 2186, 3269, 3555
BseMI GCAATG 7 cut(s) 612, 1725, 2148, 2958, 3387, 3473, 3549
BseMII CTCAG 8 cut(s) 1158, 1437, 1614, 1921, 2724, 3021, 3314, 3510
BseRI GAGGAG 3 cut(s) 30, 33, 1442
BseSI GKGCMC 4 cut(s) 1740, 2391, 3459, 3610
BseYI CCCAGC 1 cut(s) 617
BsgI GTGCAG 6 cut(s) 1175, 2333, 2528, 2667, 2833, 3167
Bsh1236I CGCG 3 cut(s) 660, 866, 3276
BshFI GGCC 6 cut(s) 296, 593, 921, 1968, 2105, 3240
BshNI GGYRCC 2 cut(s) 1134, 2432
BshVI ATCGAT 1 cut(s) 1059
BsiHKAI GWGCWC 6 cut(s) 1452, 1740, 1852, 2391, 3459, 3610
BsiHKCI CYCGRG 3 cut(s) 1579, 2120, 3055
BsiSI CCGG 6 cut(s) 1278, 1790, 1921, 1969, 3140, 3183
BslFI GGGAC 4 cut(s) 479, 640, 1531, 1777
BslI CCNNNNNNNGG 7 cut(s) 403, 1212, 1277, 2101, 2186, 3269, 3555
BsmAI GTCTC 4 cut(s) 279, 1028, 2965, 3153
BsmFI GGGAC 4 cut(s) 479, 640, 1531, 1777
BsmI GAATGC 4 cut(s) 995, 997, 1720, 1995
BsnI GGCC 6 cut(s) 296, 593, 921, 1968, 2105, 3240
Bso31I GGTCTC 2 cut(s) 1028, 2965
BsoBI CYCGRG 3 cut(s) 1579, 2120, 3055
Bsp119I TTCGAA 1 cut(s) 3025
Bsp1286I GDGCHC 6 cut(s) 1452, 1740, 1852, 2391, 3459, 3610
Bsp13I TCCGGA 2 cut(s) 1789, 3182
Bsp1720I GCTNAGC 1 cut(s) 1907
Bsp19I CCATGG 1 cut(s) 489
BspANI GGCC 6 cut(s) 296, 593, 921, 1968, 2105, 3240
BspCNI CTCAG 8 cut(s) 1159, 1438, 1615, 1920, 2725, 3020, 3315, 3509
BspDI ATCGAT 1 cut(s) 1059
BspEI TCCGGA 2 cut(s) 1789, 3182
BspFNI CGCG 3 cut(s) 660, 866, 3276
BspHI TCATGA 4 cut(s) 192, 948, 1768, 2047
BspMAI CTGCAG 2 cut(s) 316, 2158
BspMI ACCTGC 2 cut(s) 1827, 2327
BspQI GCTCTTC 1 cut(s) 113
BspT104I TTCGAA 1 cut(s) 3025
BspT107I GGYRCC 2 cut(s) 1134, 2432
BspTNI GGTCTC 2 cut(s) 1028, 2965
BsrBI CCGCTC 1 cut(s) 190
BsrDI GCAATG 7 cut(s) 612, 1725, 2148, 2958, 3387, 3473, 3549
BsrFI RCCGGY 2 cut(s) 1277, 1968
BssAI RCCGGY 2 cut(s) 1277, 1968
BssECI CCNNGG 9 cut(s) 489, 658, 1206, 1485, 1486, 2121, 2493, 3000, 3417
BssNI GRCGYC 2 cut(s) 2490, 3492
BssSI CACGAG 1 cut(s) 2036
BssT1I CCWWGG 4 cut(s) 489, 1206, 2493, 3000
Bst2BI CACGAG 1 cut(s) 2036
Bst2UI CCWGG 4 cut(s) 243, 1487, 2001, 3405
Bst6I CTCTTC 4 cut(s) 7, 113, 3368, 3417
BstACI GRCGYC 2 cut(s) 2490, 3492
BstAPI GCANNNNNTGC 2 cut(s) 2693, 3614
BstBI TTCGAA 1 cut(s) 3025
BstC8I GCNNGC 9 cut(s) 360, 685, 750, 870, 1452, 1970, 2103, 3041, 3401
BstDSI CCRYGG 2 cut(s) 489, 658
BstENI CCTNNNNNAGG 2 cut(s) 401, 3267
BstF5I GGATG 8 cut(s) 345, 462, 1417, 1624, 2464, 2706, 3148, 3619
BstFNI CGCG 3 cut(s) 660, 866, 3276
BstH2I RGCGCY 1 cut(s) 1614
BstHHI GCGC 6 cut(s) 868, 1613, 1693, 2079, 2444, 3688
BstMAI GTCTC 4 cut(s) 279, 1028, 2965, 3153
BstNI CCWGG 4 cut(s) 243, 1487, 2001, 3405
BstNSI RCATGY 1 cut(s) 336
BstSCI CCNGG 5 cut(s) 241, 1485, 1999, 3138, 3403
BstSFI CTRYAG 3 cut(s) 312, 323, 2154
BstSLI GKGCMC 4 cut(s) 1740, 2391, 3459, 3610
BstUI CGCG 3 cut(s) 660, 866, 3276
BstX2I RGATCY 3 cut(s) 884, 1158, 1786
BstXI CCANNNNNNTGG 2 cut(s) 1207, 2683
BstYI RGATCY 3 cut(s) 884, 1158, 1786
Bsu15I ATCGAT 1 cut(s) 1059
Bsu36I CCTNAGG 2 cut(s) 398, 2058
BsuI GTATCC 2 cut(s) 1870, 3138
BsuRI GGCC 6 cut(s) 296, 593, 921, 1968, 2105, 3240
BsuTUI ATCGAT 1 cut(s) 1059
BtgI CCRYGG 2 cut(s) 489, 658
BtgZI GCGATG 1 cut(s) 1679
BtrI CACGTC 2 cut(s) 1123, 1153
BtsCI GGATG 8 cut(s) 345, 462, 1417, 1624, 2464, 2706, 3148, 3619
BtsI GCAGTG 5 cut(s) 1200, 1737, 2946, 3067, 3732
BveI ACCTGC 2 cut(s) 1827, 2327
Cac8I GCNNGC 9 cut(s) 360, 685, 750, 870, 1452, 1970, 2103, 3041, 3401
CaiI CAGNNNCTG 1 cut(s) 683
CciI TCATGA 4 cut(s) 192, 948, 1768, 2047
CfoI GCGC 6 cut(s) 868, 1613, 1693, 2079, 2444, 3688
Cfr10I RCCGGY 2 cut(s) 1277, 1968
Cfr13I GGNCC 3 cut(s) 2003, 2774, 3571
Cfr42I CCGCGG 1 cut(s) 661
ClaI ATCGAT 1 cut(s) 1059
CseI GACGC 3 cut(s) 2498, 3500, 3650
Csp6I GTAC 9 cut(s) 289, 382, 763, 1135, 1286, 2171, 2665, 2792, 3278
CviQI GTAC 9 cut(s) 289, 382, 763, 1135, 1286, 2171, 2665, 2792, 3278
EaeI YGGCCR 4 cut(s) 294, 591, 1966, 2103
Eam1104I CTCTTC 4 cut(s) 7, 113, 3368, 3417
EarI CTCTTC 4 cut(s) 7, 113, 3368, 3417
Ecl136II GAGCTC 1 cut(s) 1450
Eco130I CCWWGG 4 cut(s) 489, 1206, 2493, 3000
Eco147I AGGCCT 1 cut(s) 3240
Eco24I GRGCYC 1 cut(s) 1452
Eco31I GGTCTC 2 cut(s) 1028, 2965
Eco32I GATATC 2 cut(s) 159, 264
Eco47I GGWCC 3 cut(s) 2003, 2774, 3571
Eco47III AGCGCT 1 cut(s) 1612
Eco53kI GAGCTC 1 cut(s) 1450
Eco57I CTGAAG 2 cut(s) 1592, 2603
Eco81I CCTNAGG 2 cut(s) 398, 2058
Eco88I CYCGRG 3 cut(s) 1579, 2120, 3055
EcoICRI GAGCTC 1 cut(s) 1450
EcoNI CCTNNNNNAGG 2 cut(s) 401, 3267
EcoO109I RGGNCCY 1 cut(s) 3571
EcoRI GAATTC 3 cut(s) 1584, 2358, 3027
EcoRII CCWGG 4 cut(s) 241, 1485, 1999, 3403
EcoRV GATATC 2 cut(s) 159, 264
EcoT14I CCWWGG 4 cut(s) 489, 1206, 2493, 3000
EcoT22I ATGCAT 1 cut(s) 997
EcoT38I GRGCYC 1 cut(s) 1452
ErhI CCWWGG 4 cut(s) 489, 1206, 2493, 3000
FalI AAGNNNNNCTT 4 cut(s) 2601, 2633, 2915, 2947
FaqI GGGAC 4 cut(s) 479, 640, 1531, 1777
FauI CCCGC 3 cut(s) 665, 1220, 3212
FbaI TGATCA 2 cut(s) 894, 3109
FblI GTMKAC 1 cut(s) 1235
FokI GGATG 8 cut(s) 352, 469, 1424, 1631, 2471, 2693, 3155, 3626
FriOI GRGCYC 1 cut(s) 1452
FspBI CTAG 7 cut(s) 366, 432, 1250, 2141, 2253, 2973, 3284
FspI TGCGCA 2 cut(s) 1692, 3687
GlaI GCGC 6 cut(s) 867, 1612, 1692, 2078, 2443, 3687
GsaI CCCAGC 1 cut(s) 621
GsuI CTGGAG 2 cut(s) 327, 459
HaeII RGCGCY 1 cut(s) 1614
HaeIII GGCC 6 cut(s) 296, 593, 921, 1968, 2105, 3240
HapII CCGG 6 cut(s) 1278, 1790, 1921, 1969, 3140, 3183
HgaI GACGC 3 cut(s) 2498, 3500, 3650
HhaI GCGC 6 cut(s) 868, 1613, 1693, 2079, 2444, 3688
Hin1I GRCGYC 2 cut(s) 2490, 3492
Hin6I GCGC 6 cut(s) 866, 1611, 1691, 2077, 2442, 3686
HinP1I GCGC 6 cut(s) 866, 1611, 1691, 2077, 2442, 3686
HincII GTYRAC 3 cut(s) 1236, 1779, 3228
HindII GTYRAC 3 cut(s) 1236, 1779, 3228
HpaII CCGG 6 cut(s) 1278, 1790, 1921, 1969, 3140, 3183
Hpy99I CGWCG 1 cut(s) 3494
HpyCH4IV ACGT 4 cut(s) 132, 1122, 1152, 3453
HpySE526I ACGT 4 cut(s) 132, 1122, 1152, 3453
Hsp92I GRCGYC 2 cut(s) 2490, 3492
HspAI GCGC 6 cut(s) 866, 1611, 1691, 2077, 2442, 3686
Kpn2I TCCGGA 2 cut(s) 1789, 3182
KpnI GGTACC 1 cut(s) 1138
KroI GCCGGC 1 cut(s) 1968
KroNI GCCGGC 1 cut(s) 1970
Ksp22I TGATCA 2 cut(s) 894, 3109
KspI CCGCGG 1 cut(s) 661
LguI GCTCTTC 1 cut(s) 113
LmnI GCTCC 8 cut(s) 308, 1847, 1917, 2144, 2353, 2876, 2986, 3710
MaeI CTAG 7 cut(s) 366, 432, 1250, 2141, 2253, 2973, 3284
MaeII ACGT 4 cut(s) 132, 1122, 1152, 3453
MaeIII GTNAC 7 cut(s) 57, 245, 343, 814, 963, 2486, 3412
MbiI CCGCTC 1 cut(s) 190
MfeI CAATTG 3 cut(s) 753, 1554, 2007
MflI RGATCY 3 cut(s) 884, 1158, 1786
MhlI GDGCHC 6 cut(s) 1452, 1740, 1852, 2391, 3459, 3610
MlsI TGGCCA 2 cut(s) 296, 593
MluNI TGGCCA 2 cut(s) 296, 593
MlyI GAGTC 9 cut(s) 190, 822, 1634, 1759, 2485, 2981, 2999, 3047, 3629
MmeI TCCRAC 5 cut(s) 130, 317, 1076, 3015, 3184
Mox20I TGGCCA 2 cut(s) 296, 593
Mph1103I ATGCAT 1 cut(s) 997
MroI TCCGGA 2 cut(s) 1789, 3182
MroNI GCCGGC 1 cut(s) 1968
MroXI GAANNNNTTC 4 cut(s) 995, 2321, 3357, 3375
MscI TGGCCA 2 cut(s) 296, 593
MslI CAYNNNNRTG 3 cut(s) 1730, 2213, 2951
Msp20I TGGCCA 2 cut(s) 296, 593
MspA1I CMGCKG 2 cut(s) 660, 3219
MspI CCGG 6 cut(s) 1278, 1790, 1921, 1969, 3140, 3183
MspR9I CCNGG 5 cut(s) 243, 1487, 2001, 3140, 3405
MunI CAATTG 3 cut(s) 753, 1554, 2007
Mva1269I GAATGC 4 cut(s) 995, 997, 1720, 1995
MvaI CCWGG 4 cut(s) 243, 1487, 2001, 3405
MvnI CGCG 3 cut(s) 660, 866, 3276
NaeI GCCGGC 1 cut(s) 1970
NciI CCSGG 1 cut(s) 3140
NcoI CCATGG 1 cut(s) 489
NgoMIV GCCGGC 1 cut(s) 1968
NmuCI GTSAC 5 cut(s) 57, 245, 814, 963, 2486
NsbI TGCGCA 2 cut(s) 1692, 3687
NsiI ATGCAT 1 cut(s) 997
NspI RCATGY 1 cut(s) 336
NspV TTCGAA 1 cut(s) 3025
PaeR7I CTCGAG 1 cut(s) 3055
PagI TCATGA 4 cut(s) 192, 948, 1768, 2047
PaqCI CACCTGC 2 cut(s) 1827, 2327
PasI CCCWGGG 1 cut(s) 1486
PceI AGGCCT 1 cut(s) 3240
PciSI GCTCTTC 1 cut(s) 113
PcsI WCGNNNNNNNCGW 2 cut(s) 650, 1065
PctI GAATGC 4 cut(s) 995, 997, 1720, 1995
PdiI GCCGGC 1 cut(s) 1970
PdmI GAANNNNTTC 4 cut(s) 995, 2321, 3357, 3375
PfeI GAWTC 7 cut(s) 1366, 2066, 2249, 2599, 3266, 3371, 3543
PleI GAGTC 9 cut(s) 190, 822, 1633, 1759, 2484, 2981, 2999, 3047, 3628
PpsI GAGTC 9 cut(s) 190, 822, 1633, 1759, 2484, 2981, 2999, 3047, 3628
PpuMI RGGWCCY 1 cut(s) 3571
Psp124BI GAGCTC 1 cut(s) 1452
Psp5II RGGWCCY 1 cut(s) 3571
Psp6I CCWGG 4 cut(s) 241, 1485, 1999, 3403
PspFI CCCAGC 1 cut(s) 617
PspGI CCWGG 4 cut(s) 241, 1485, 1999, 3403
PspPI GGNCC 3 cut(s) 2003, 2774, 3571
PspPPI RGGWCCY 1 cut(s) 3571
PspXI VCTCGAGB 1 cut(s) 3055
PstI CTGCAG 2 cut(s) 316, 2158
PstNI CAGNNNCTG 1 cut(s) 683
PsuI RGATCY 3 cut(s) 884, 1158, 1786
RsaI GTAC 9 cut(s) 290, 383, 764, 1136, 1287, 2172, 2666, 2793, 3279
RsaNI GTAC 9 cut(s) 289, 382, 763, 1135, 1286, 2171, 2665, 2792, 3278
RseI CAYNNNNRTG 3 cut(s) 1730, 2213, 2951
SacI GAGCTC 1 cut(s) 1452
SacII CCGCGG 1 cut(s) 661
SalI GTCGAC 1 cut(s) 1234
SapI GCTCTTC 1 cut(s) 113
Sau96I GGNCC 3 cut(s) 2003, 2774, 3571
ScaI AGTACT 2 cut(s) 1287, 2172
SchI GAGTC 9 cut(s) 190, 822, 1634, 1759, 2485, 2981, 2999, 3047, 3629
ScrFI CCNGG 5 cut(s) 243, 1487, 2001, 3140, 3405
SduI GDGCHC 6 cut(s) 1452, 1740, 1852, 2391, 3459, 3610
SfcI CTRYAG 3 cut(s) 312, 323, 2154
Sfr274I CTCGAG 1 cut(s) 3055
Sfr303I CCGCGG 1 cut(s) 661
SfuI TTCGAA 1 cut(s) 3025
SgrBI CCGCGG 1 cut(s) 661
SinI GGWCC 3 cut(s) 2003, 2774, 3571
SlaI CTCGAG 1 cut(s) 3055
SmiMI CAYNNNNRTG 3 cut(s) 1730, 2213, 2951
SmlI CTYRAG 3 cut(s) 3055, 3480, 3566
SmoI CTYRAG 3 cut(s) 3055, 3480, 3566
SseBI AGGCCT 1 cut(s) 3240
SspI AATATT 1 cut(s) 837
SspMI CTAG 7 cut(s) 366, 432, 1250, 2141, 2253, 2973, 3284
SstI GAGCTC 1 cut(s) 1452
StuI AGGCCT 1 cut(s) 3240
StyD4I CCNGG 5 cut(s) 241, 1485, 1999, 3138, 3403
StyI CCWWGG 4 cut(s) 489, 1206, 2493, 3000
TaiI ACGT 4 cut(s) 135, 1125, 1155, 3456
TatI WGTACW 5 cut(s) 288, 381, 762, 1285, 2170
TauI GCSGC 3 cut(s) 190, 574, 2108
TfiI GAWTC 7 cut(s) 1366, 2066, 2249, 2599, 3266, 3371, 3543
TseFI GTSAC 5 cut(s) 57, 245, 814, 963, 2486
Tsp45I GTSAC 5 cut(s) 57, 245, 814, 963, 2486
TspGWI ACGGA 4 cut(s) 1860, 2471, 2766, 3303
VneI GTGCAC 4 cut(s) 1736, 2387, 3455, 3606
VpaK11BI GGWCC 3 cut(s) 2003, 2774, 3571
XagI CCTNNNNNAGG 2 cut(s) 401, 3267
XapI RAATTY 7 cut(s) 1466, 1584, 2317, 2358, 2422, 2627, 3027
XbaI TCTAGA 1 cut(s) 2252
XceI RCATGY 1 cut(s) 336
XcmI CCANNNNNNNNNTGG 1 cut(s) 496
XhoI CTCGAG 1 cut(s) 3055
XmiI GTMKAC 1 cut(s) 1235
XmnI GAANNNNTTC 4 cut(s) 995, 2321, 3357, 3375
XspI CTAG 7 cut(s) 366, 432, 1250, 2141, 2253, 2973, 3284
ZrmI AGTACT 2 cut(s) 1287, 2172
Zsp2I ATGCAT 1 cut(s) 997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.