FvH4_6g19120

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001944
Physical Location & Seq
Reverse (-)
12649407 .. 12654574
5168 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g19120.t1

Sequence Viewer

Length: 3792 bp
ATGGAGCAAGAAGGAAAGATGACAATGAACAAGAACAAAGTAAGAGATCAAGACAAGCAGAGTGTTCCATTTTACAAGCTGTTTATGTTCGCCGACAAATACGACGTCGCACTGATGATCATCGGAAGTATAGGTGCGATCGGAAATGGGTTATCACAACCACTCATGACGCTGGTCTTTGGTGGTCTTATAAATACTTTCGGAGCTACCGACCCGGCTCATATTGTCCCCACTGTTTCTCAGGTGTCTCTGAAGTTTTTGTACTTGGCTATTGGTACAGGCGTTGCTGCATTTTTGCAGGTCTCGTGTTGGATGGTGACCGGGGAAAGACAAGCCACTCGAATTCGAGGCTTGTACTTGAAAACTATACTAAGACAAGATATCGGATTTTTCGACACTCAAACAAATACCGGAGAGATCATTGGGAGAATGTCAGGTGACACCATTCTCATTCAAAATGCCATGGGAGAAAAGGTGGGGAAGTTCATACAACTTCTTTCAACTTTTCTTGGGGGTTTTGTAATTGCTTTTCTCAAAGGATGGCTGCTCACATTGGTTTTGCTATCATGTGTTCCCGCACTTGTCATTGCTGGTGCACTCATGTCTATTGTAGTTTCGAAAATGTCAACACGCGGGCAACGTGCTTATGCGGAAGCTGGCGCTATAGTTGAGCAGACTGTTGGAGCCATTAGAACAGTTGCATCTTTTACCGGAGAGAAGCAAGCGATAGATAAATACAATCAGAAGTTGAAAATTGCATACAATAACACGGTTCAACAAGGATTAGCCACAGGGGTTGGACTTGGTGCATTCATGCTTATTGTCTTTAGCACTTACGGACTTGCCATATGGTACGGATCCAAAATGATCATTGAGAAAGGCTACAATGGGGGCCAAGTCATCAATGTTCTATTTGCAATGATGAGTGGTGGAATGTCACTAGGACAGACATCTCCGTCTCTCAATGCATTTACAGCAGGAAAAGCTGCTGCATACAAAATGTTTGAAACTATCAAGCGAACACCAACAATCGATCCTTATGATACTACTGGAATTGTGTTGGAAGATGTTAAAGGAGAGGTCGAACTTAAAGATGTGCATTTTAGGTATCCTGCTAGACCAGATGTGCAGATCTTTGCCGGATTCTCGTTGCATGTTCCGAGTGGCACAACGACTGCTTTGGTTGGGCAGAGTGGAAGTGGGAAATCAACTGTAATAGGCCTGGTAGAGAGGTTTTATGATCCAGAAGCCGGTGAAGTGTTTATTGATGGAGTGAATTTGAAGAAGTTGCATCTTAGAGCAATAAGGGAGAGGATTGGCCTTGTAAGTCAAGAACCAAATCTCTTTACAACAACGATAAGACAAAATATAGCATATGGGAAAGAAAATGCAACTGAAGAGGAGATTAGAAGAGCCATGGAGCTTGCAAATGCAGCAAAATTCATTGATAAGCTGCCCGAGGGGCTCAACACAATGATAGGTGGGACATCATTATCAGGTGGGCAAAAGCAAAGAATTGCAATTGCTAGAGCCATTTTGAAGAACCCTAGAATTTTGCTTCTTGACGAAGCAACAAGTGCTCTGGATACTGAGTCTGAACGAATAGTTCAAGATGCACTTGTCAGACTGATGTCAAATAGGACAACTATAGTTGTTGCTCATCGCTTGACAACGATCAGAAATGCTGATGCAATAGCAGTGGTGCATAAAGGGAAAATTGTAGAAAAAGGAACTCACGACGAATTGACCAAAGATCCAGAAGGAGCTTATAGCCAACTAGTTCGGCTACAGGAGGGAGCTAAAGAAAAAGATGCAGAGTCAGATGGTCTAGATAATGGTTTAGATATAGATAAGACCATGCTTAGCTTTGGCAGCCAAAGATTATCAACCGGGAAATCTTTAAGCATAGGTTCATTATCTCGCCGGTTGTCGTCCACTATTAGCAGCTTTGGTACTCCTGCTCTAGTTAATTGCCAAGTGACTGAAGTAGGAGATGAGGGGAGCCTTGAAAAAACTAAGATTGATCCCGAAGAGCGTAAAACAGTTTCCATCAAAAGGTTGGCGATGATGAACAAAAATGAGCTTCCAGTTTTGCTACTTGGAGCCATTGCAGCAGCTGGTCATGGAGTAATTTTTCCTGTCTTCGGTGTACTACTCTCAAAAGCGATTAAAATGTTCTACGAACCGCATAGTGTGCTCAGGGAAGACTCAAAGACATGGGCCGGAGTGTATGTAGGGATAGGTTGCTTTGGGATGGTCGTCGTTCCAGTGCAGAATTTCTTATTTGGAGTTGCAGGTGGAAAATTGATAGAGCAAATTCGGTCCTTGACATTCCAAAAGGTTGTGCACCAACAAATCAGTTGGTTTGATGATCCTGCAAATTCAAGTGGTGCCATTGGTGCAAGGTTGTCTTCTGATGCTTCAACCGTAAAGGCTCTTGTTGGTGACGGCTTAGCCTTGATTACGCAGAATATCGCAACCATCATAGCAGGGTTGGTTATAGGTTTCACAGCTAACTGGAAATTGGCGCTTCTGATTTTAGGCGTGTCACCATTATTGATTATTCAAGGACTACTTCAGACAAAATTTCTTAAAGGGTTTAGTGGAGACGCCAAGGCTATGTATGAAGAAGCAAGTCAAGTTGCAAATGATGCAATTGGTAGCATTCGAACTGTTGCGTCATTTTGTTCTGAAAAGAAAGTGATGGATGCTTATGAGAAGAAGTGTGAGGGTCCTATGAAGCAAGGAGTACGATTAGGAGTAGTGAGTGGTGTAGGTTTTGGCTTCTCTTTCTTTGTAATGTTCTGCACCAATGCACTAATTTTCTACATTGGAGCTATTCTTGTGAAAAATGGCCAAGCTAAATTTGACCAAGTATTCATGGTGTTCTTTGCTTTGACAATCTCAGCAGTTGGAGTTTCAGAATCTAGTGGTATGGCTCCTGACTCCAACAAAGCAAAAGACTCAGCAGCTTCAATATTTAAGATTCTTGACAGTAAGCCTGAGATCGACTCGAGCAGCGATGAAGGCTTAACACTACCGAGCGTTAGTGGAAAAATTGATCTCGAACATGTCAGCTTCAAGTATCCCACAAGGCCGGATGTGCAGATATTTAGAGACATTTGCTTGAGTATCCCCTCTGGAAAGACTGTTGCTTTGGTTGGAGAGAGTGGCAGTGGGAAGTCGACAGTGATTGGCCTCATAGAGCGATTCTATAACCCCGACTCGGGTCGTGTACTATTAGATGGAGTTGAAATCAATAAGTTCAAGATAAACTGGCTGAGGCAGCAAATAGGTTTGGTTGGTCAAGAACCAATTCTCTTTAACGAATCCATTCGAACTAACATTGCTTATGGCAAGCGAGGAGATGTAACTGAAGAAGAGATCATTGCAGCCACAAAAGCATCAAATGCTCACAACTTCATTTCTTCACTGCCTCAAGGCTATGACACCTCTGTTGGAGAACGAGGGGTTCAGTTATCTGGAGGGCAGAAACAAAGAATAGCCATTGCAATGGCTATCTTAAAAGACCCTAGAATTCTTTTACTCGATGAAGCTACAAGTGCATTGGATGCAGAGTCAGAACGCATTGTACAAGATGCTTTGGATAGAGTGATGGTGAATAGAACAACTGTTGTCGTTGCCCACCGCCTTACCACAATTAAAGGCGCAGACATGATAGCTGTGGTCAAGAACGGCGTGATTGCTGAGAAAGGTAGCCATGATGTTCTAATGAAGATCACCGATGGTGCTTATGCTTCTTTGGTGGCACTCCATTCAAGCTCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1264

Amino Acids

136.02

Weight (kDa)

8.66

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 39 - 310 9.1e-60 ABC transporter transmembrane region
ABC_tran PF00005 381 - 526 4.7e-31 ABC transporter
ABC_membrane PF00664 698 - 970 4.3e-59 ABC transporter transmembrane region
ABC_tran PF00005 1039 - 1188 2.4e-32 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 191
AarI CACCTGC 1 cut(s) 2285
AasI GACNNNNNNGTC 1 cut(s) 955
AatII GACGTC 1 cut(s) 108
Acc36I ACCTGC 2 cut(s) 289, 2285
AccB1I GGYRCC 1 cut(s) 2390
AccI GTMKAC 1 cut(s) 3185
AccII CGCG 1 cut(s) 633
AciI CCGC 5 cut(s) 576, 633, 650, 2186, 3649
AclWI GGATC 7 cut(s) 852, 865, 1028, 1235, 1748, 2018, 2366
AcoI YGGCCR 1 cut(s) 2854
AcuI CTGAAG 5 cut(s) 272, 1416, 2004, 2561, 3396
AcyI GRCGYC 2 cut(s) 105, 2610
AfaI GTAC 9 cut(s) 263, 277, 356, 854, 1954, 2151, 2751, 3237, 3594
AfiI CCNNNNNNNGG 5 cut(s) 1250, 2055, 2144, 3226, 3227
AflIII ACRYGT 1 cut(s) 3070
AhlI ACTAGT 1 cut(s) 1777
AjnI CCWGG 1 cut(s) 1221
Alw21I GWGCWC 4 cut(s) 598, 1582, 2199, 2349
Alw26I GTCTC 5 cut(s) 252, 307, 963, 2601, 3111
Alw44I GTGCAC 2 cut(s) 594, 2345
AlwI GGATC 7 cut(s) 852, 865, 1028, 1235, 1748, 2018, 2366
AlwNI CAGNNNCTG 1 cut(s) 2117
Ama87I CYCGRG 3 cut(s) 1457, 3013, 3226
AoxI GGCC 7 cut(s) 892, 1219, 1318, 2220, 2854, 3095, 3196
ApaLI GTGCAC 2 cut(s) 594, 2345
ArsI GACNNNNNNTTYG 4 cut(s) 90, 122, 3139, 3171
Asp700I GAANNNNTTC 2 cut(s) 3315, 3333
AspLEI GCGC 3 cut(s) 662, 2530, 3671
AspS9I GGNCC 4 cut(s) 892, 2220, 2322, 2732
AsuC2I CCSGG 3 cut(s) 215, 322, 1891
AsuHPI GGTGA 7 cut(s) 328, 449, 1265, 2456, 2541, 3631, 3733
AsuII TTCGAA 3 cut(s) 617, 2668, 3337
AvaI CYCGRG 3 cut(s) 1457, 3013, 3226
AvaII GGWCC 2 cut(s) 2322, 2732
BaeGI GKGCMC 2 cut(s) 598, 2349
BaeI ACNNNNGTAYC 2 cut(s) 267, 300
BalI TGGCCA 1 cut(s) 2856
BamHI GGATCC 1 cut(s) 857
BanI GGYRCC 1 cut(s) 2390
BanII GRGCYC 1 cut(s) 1467
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
BauI CACGAG 1 cut(s) 304
BbsI GAAGAC 3 cut(s) 2134, 2211, 2403
Bbv12I GWGCWC 4 cut(s) 598, 1582, 2199, 2349
BbvCI CCTCAGC 1 cut(s) 3281
BceAI ACGGC 2 cut(s) 2464, 3712
BciT130I CCWGG 1 cut(s) 1223
BciVI GTATCC 4 cut(s) 1119, 1579, 3096, 3143
BclI TGATCA 2 cut(s) 117, 867
BcnI CCSGG 3 cut(s) 215, 322, 1891
BcoDI GTCTC 5 cut(s) 252, 307, 963, 2601, 3111
BcuI ACTAGT 1 cut(s) 1777
BfaI CTAG 9 cut(s) 941, 1116, 1527, 1548, 1778, 1829, 1964, 2928, 3534
BfmI CTRYAG 3 cut(s) 663, 1647, 1787
BfoI RGCGCY 2 cut(s) 663, 2531
BfuAI ACCTGC 2 cut(s) 289, 2285
BfuI GTATCC 4 cut(s) 1119, 1579, 3096, 3143
BglI GCCNNNNNGGC 1 cut(s) 1462
BglII AGATCT 1 cut(s) 1131
BlpI GCTNAGC 2 cut(s) 1862, 2452
Bme1390I CCNGG 4 cut(s) 215, 322, 1223, 1891
Bme18I GGWCC 2 cut(s) 2322, 2732
BmeT110I CYCGRG 3 cut(s) 1457, 3013, 3226
BmgT120I GGNCC 4 cut(s) 892, 2220, 2322, 2732
BmiI GGNNCC 8 cut(s) 685, 859, 893, 2003, 2104, 2392, 2733, 2940
BmrFI CCNGG 4 cut(s) 215, 322, 1223, 1891
BoxI GACNNNNGTC 3 cut(s) 173, 1630, 3228
BpiI GAAGAC 3 cut(s) 2134, 2211, 2403
BplI GAGNNNNNCTC 2 cut(s) 2996, 3028
BpmI CTGGAG 1 cut(s) 3504
Bpu10I CCTNAGC 2 cut(s) 2198, 3281
Bpu1102I GCTNAGC 2 cut(s) 1862, 2452
Bpu14I TTCGAA 3 cut(s) 617, 2668, 3337
BpuEI CTTGAG 2 cut(s) 3148, 3423
BpuMI CCSGG 3 cut(s) 215, 322, 1891
Bsa29I ATCGAT 1 cut(s) 1032
BsaBI GATNNNNATC 1 cut(s) 119
BsaHI GRCGYC 2 cut(s) 105, 2610
BsaI GGTCTC 1 cut(s) 307
BsaJI CCNNGG 5 cut(s) 321, 462, 1416, 1458, 2613
BsaWI WCCGGW 2 cut(s) 410, 710
BsaXI ACNNNNNCTCC 4 cut(s) 459, 489, 2217, 2247
Bsc4I CCNNNNNNNGG 5 cut(s) 1250, 2055, 2144, 3226, 3227
Bse118I RCCGGY 2 cut(s) 1250, 1923
Bse1I ACTGG 5 cut(s) 1054, 2087, 2267, 2522, 3281
Bse3DI GCAATG 7 cut(s) 585, 924, 2106, 3345, 3387, 3507, 3519
Bse8I GATNNNNATC 1 cut(s) 119
BseBI CCWGG 1 cut(s) 1223
BseCI ATCGAT 1 cut(s) 1032
BseDI CCNNGG 5 cut(s) 321, 462, 1416, 1458, 2613
BseGI GGATG 6 cut(s) 318, 545, 2259, 2713, 3106, 3577
BseJI GATNNNNATC 1 cut(s) 119
BseLI CCNNNNNNNGG 5 cut(s) 1250, 2055, 2144, 3226, 3227
BseMI GCAATG 7 cut(s) 585, 924, 2106, 3345, 3387, 3507, 3519
BseMII CTCAG 8 cut(s) 254, 1581, 2212, 2919, 2979, 2994, 3272, 3699
BseNI ACTGG 5 cut(s) 1054, 2087, 2267, 2522, 3281
BseRI GAGGAG 2 cut(s) 1415, 3378
BseSI GKGCMC 2 cut(s) 598, 2349
BsgI GTGCAG 4 cut(s) 1148, 2291, 2791, 3125
Bsh1236I CGCG 1 cut(s) 633
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 7 cut(s) 894, 1221, 1320, 2222, 2856, 3097, 3198
BshNI GGYRCC 1 cut(s) 2390
BshVI ATCGAT 1 cut(s) 1032
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 4 cut(s) 598, 1582, 2199, 2349
BsiHKCI CYCGRG 3 cut(s) 1457, 3013, 3226
BslFI GGGAC 2 cut(s) 212, 1498
BslI CCNNNNNNNGG 5 cut(s) 1250, 2055, 2144, 3226, 3227
BsmAI GTCTC 5 cut(s) 252, 307, 963, 2601, 3111
BsmBI CGTCTC 2 cut(s) 963, 2601
BsmFI GGGAC 2 cut(s) 212, 1498
BsmI GAATGC 2 cut(s) 809, 2664
BsnI GGCC 7 cut(s) 894, 1221, 1320, 2222, 2856, 3097, 3198
Bso31I GGTCTC 1 cut(s) 307
BsoBI CYCGRG 3 cut(s) 1457, 3013, 3226
Bsp119I TTCGAA 3 cut(s) 617, 2668, 3337
Bsp1286I GDGCHC 5 cut(s) 598, 1467, 1582, 2199, 2349
Bsp1407I TGTACA 1 cut(s) 3592
Bsp1720I GCTNAGC 2 cut(s) 1862, 2452
Bsp19I CCATGG 2 cut(s) 462, 1416
BspACI CCGC 5 cut(s) 576, 633, 650, 2186, 3649
BspANI GGCC 7 cut(s) 894, 1221, 1320, 2222, 2856, 3097, 3198
BspCNI CTCAG 8 cut(s) 253, 1582, 2211, 2918, 2978, 2995, 3273, 3700
BspDI ATCGAT 1 cut(s) 1032
BspFNI CGCG 1 cut(s) 633
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 8 cut(s) 685, 859, 893, 2003, 2104, 2392, 2733, 2940
BspMI ACCTGC 2 cut(s) 289, 2285
BspPI GGATC 7 cut(s) 852, 865, 1028, 1235, 1748, 2018, 2366
BspQI GCTCTTC 2 cut(s) 1405, 2025
BspT104I TTCGAA 3 cut(s) 617, 2668, 3337
BspT107I GGYRCC 1 cut(s) 2390
BspTNI GGTCTC 1 cut(s) 307
BsrDI GCAATG 7 cut(s) 585, 924, 2106, 3345, 3387, 3507, 3519
BsrFI RCCGGY 2 cut(s) 1250, 1923
BsrGI TGTACA 1 cut(s) 3592
BsrI ACTGG 5 cut(s) 1054, 2087, 2267, 2522, 3281
BssAI RCCGGY 2 cut(s) 1250, 1923
BssECI CCNNGG 5 cut(s) 321, 462, 1416, 1458, 2613
BssNI GRCGYC 2 cut(s) 105, 2610
BssSI CACGAG 1 cut(s) 304
BssT1I CCWWGG 3 cut(s) 462, 1416, 2613
Bst2BI CACGAG 1 cut(s) 304
Bst2UI CCWGG 1 cut(s) 1223
Bst6I CTCTTC 4 cut(s) 1392, 1405, 2025, 3375
BstACI GRCGYC 2 cut(s) 105, 2610
BstAPI GCANNNNNTGC 5 cut(s) 1577, 2194, 2651, 3410, 3572
BstAUI TGTACA 1 cut(s) 3592
BstBI TTCGAA 3 cut(s) 617, 2668, 3337
BstC8I GCNNGC 5 cut(s) 635, 658, 723, 1425, 3359
BstDSI CCRYGG 2 cut(s) 462, 1416
BstEII GGTNACC 1 cut(s) 316
BstF5I GGATG 6 cut(s) 318, 545, 2259, 2713, 3106, 3577
BstFNI CGCG 1 cut(s) 633
BstH2I RGCGCY 2 cut(s) 663, 2531
BstHHI GCGC 3 cut(s) 662, 2530, 3671
BstMAI GTCTC 5 cut(s) 252, 307, 963, 2601, 3111
BstMCI CGRYCG 1 cut(s) 141
BstNI CCWGG 1 cut(s) 1223
BstNSI RCATGY 2 cut(s) 1157, 3074
BstPAI GACNNNNGTC 3 cut(s) 173, 1630, 3228
BstPI GGTNACC 1 cut(s) 316
BstSCI CCNGG 4 cut(s) 213, 320, 1221, 1889
BstSFI CTRYAG 3 cut(s) 663, 1647, 1787
BstSLI GKGCMC 2 cut(s) 598, 2349
BstUI CGCG 1 cut(s) 633
BstV2I GAAGAC 3 cut(s) 2134, 2211, 2403
BstX2I RGATCY 3 cut(s) 857, 1131, 1753
BstXI CCANNNNNNTGG 1 cut(s) 3514
BstYI RGATCY 3 cut(s) 857, 1131, 1753
Bsu15I ATCGAT 1 cut(s) 1032
BsuI GTATCC 4 cut(s) 1119, 1579, 3096, 3143
BsuRI GGCC 7 cut(s) 894, 1221, 1320, 2222, 2856, 3097, 3198
BsuTUI ATCGAT 1 cut(s) 1032
BtgI CCRYGG 2 cut(s) 462, 1416
BtgZI GCGATG 3 cut(s) 1646, 2078, 3036
BtsCI GGATG 6 cut(s) 318, 545, 2259, 2713, 3106, 3577
BtsI GCAGTG 3 cut(s) 1704, 3181, 3431
BtsIMutI CAGTG 7 cut(s) 110, 231, 1704, 2274, 3181, 3195, 3431
BveI ACCTGC 2 cut(s) 289, 2285
Cac8I GCNNGC 5 cut(s) 635, 658, 723, 1425, 3359
CaiI CAGNNNCTG 1 cut(s) 2117
CciI TCATGA 1 cut(s) 165
CfoI GCGC 3 cut(s) 662, 2530, 3671
Cfr10I RCCGGY 2 cut(s) 1250, 1923
Cfr13I GGNCC 4 cut(s) 892, 2220, 2322, 2732
ClaI ATCGAT 1 cut(s) 1032
CseI GACGC 3 cut(s) 178, 2618, 2667
Csp6I GTAC 9 cut(s) 262, 276, 355, 853, 1953, 2150, 2750, 3236, 3593
CspCI CAANNNNNGTGG 4 cut(s) 778, 813, 1680, 1715
CviQI GTAC 9 cut(s) 262, 276, 355, 853, 1953, 2150, 2750, 3236, 3593
DrdI GACNNNNNNGTC 1 cut(s) 955
DseDI GACNNNNNNGTC 1 cut(s) 955
EaeI YGGCCR 1 cut(s) 2854
Eam1104I CTCTTC 4 cut(s) 1392, 1405, 2025, 3375
EarI CTCTTC 4 cut(s) 1392, 1405, 2025, 3375
Eco130I CCWWGG 3 cut(s) 462, 1416, 2613
Eco147I AGGCCT 1 cut(s) 1221
Eco24I GRGCYC 1 cut(s) 1467
Eco31I GGTCTC 1 cut(s) 307
Eco32I GATATC 1 cut(s) 382
Eco47I GGWCC 2 cut(s) 2322, 2732
Eco57I CTGAAG 5 cut(s) 272, 1416, 2004, 2561, 3396
Eco88I CYCGRG 3 cut(s) 1457, 3013, 3226
Eco91I GGTNACC 1 cut(s) 316
EcoO109I RGGNCCY 1 cut(s) 2732
EcoO65I GGTNACC 1 cut(s) 316
EcoRI GAATTC 2 cut(s) 342, 3537
EcoRII CCWGG 1 cut(s) 1221
EcoRV GATATC 1 cut(s) 382
EcoT14I CCWWGG 3 cut(s) 462, 1416, 2613
EcoT22I ATGCAT 1 cut(s) 970
EcoT38I GRGCYC 1 cut(s) 1467
ErhI CCWWGG 3 cut(s) 462, 1416, 2613
Esp3I CGTCTC 2 cut(s) 963, 2601
FalI AAGNNNNNCTT 6 cut(s) 1602, 1634, 2395, 2427, 2559, 2591
FaqI GGGAC 2 cut(s) 212, 1498
FauI CCCGC 2 cut(s) 583, 626
FauNDI CATATG 2 cut(s) 848, 1375
FbaI TGATCA 2 cut(s) 117, 867
FblI GTMKAC 1 cut(s) 3185
FokI GGATG 6 cut(s) 325, 552, 2266, 2720, 3113, 3584
FriOI GRGCYC 1 cut(s) 1467
FspBI CTAG 9 cut(s) 941, 1116, 1527, 1548, 1778, 1829, 1964, 2928, 3534
GlaI GCGC 3 cut(s) 661, 2529, 3670
GsuI CTGGAG 1 cut(s) 3504
HaeII RGCGCY 2 cut(s) 663, 2531
HaeIII GGCC 7 cut(s) 894, 1221, 1320, 2222, 2856, 3097, 3198
HgaI GACGC 3 cut(s) 178, 2618, 2667
HhaI GCGC 3 cut(s) 662, 2530, 3671
Hin1I GRCGYC 2 cut(s) 105, 2610
Hin6I GCGC 3 cut(s) 660, 2528, 3669
HinP1I GCGC 3 cut(s) 660, 2528, 3669
HincII GTYRAC 2 cut(s) 627, 3186
HindII GTYRAC 2 cut(s) 627, 3186
HphI GGTGA 7 cut(s) 328, 449, 1265, 2456, 2541, 3631, 3733
Hpy166II GTNNAC 7 cut(s) 596, 627, 1935, 2150, 2347, 3186, 3236
Hpy8I GTNNAC 7 cut(s) 596, 627, 1935, 2150, 2347, 3186, 3236
Hpy99I CGWCG 4 cut(s) 107, 110, 1742, 2264
HpyAV CCTTC 3 cut(s) 5, 1754, 3020
HpyCH4IV ACGT 2 cut(s) 105, 640
HpySE526I ACGT 2 cut(s) 105, 640
Hsp92I GRCGYC 2 cut(s) 105, 2610
HspAI GCGC 3 cut(s) 660, 2528, 3669
Ksp22I TGATCA 2 cut(s) 117, 867
LguI GCTCTTC 2 cut(s) 1405, 2025
MaeI CTAG 9 cut(s) 941, 1116, 1527, 1548, 1778, 1829, 1964, 2928, 3534
MaeII ACGT 2 cut(s) 105, 640
MaeIII GTNAC 7 cut(s) 316, 437, 936, 1978, 2444, 2547, 3370
MfeI CAATTG 2 cut(s) 1521, 2655
MflI RGATCY 3 cut(s) 857, 1131, 1753
MhlI GDGCHC 5 cut(s) 598, 1467, 1582, 2199, 2349
MlsI TGGCCA 1 cut(s) 2856
MluNI TGGCCA 1 cut(s) 2856
MlyI GAGTC 8 cut(s) 1601, 1826, 2201, 2939, 2957, 3005, 3218, 3587
MmeI TCCRAC 8 cut(s) 290, 661, 778, 1041, 2893, 2973, 3142, 3439
Mox20I TGGCCA 1 cut(s) 2856
Mph1103I ATGCAT 1 cut(s) 970
MroXI GAANNNNTTC 2 cut(s) 3315, 3333
MscI TGGCCA 1 cut(s) 2856
MslI CAYNNNNRTG 1 cut(s) 3512
Msp20I TGGCCA 1 cut(s) 2856
MspA1I CMGCKG 1 cut(s) 2117
MspR9I CCNGG 4 cut(s) 215, 322, 1223, 1891
MunI CAATTG 2 cut(s) 1521, 2655
Mva1269I GAATGC 2 cut(s) 809, 2664
MvaI CCWGG 1 cut(s) 1223
MvnI CGCG 1 cut(s) 633
NciI CCSGG 3 cut(s) 215, 322, 1891
NcoI CCATGG 2 cut(s) 462, 1416
NdeI CATATG 2 cut(s) 848, 1375
NlaIV GGNNCC 8 cut(s) 685, 859, 893, 2003, 2104, 2392, 2733, 2940
NmuCI GTSAC 6 cut(s) 316, 437, 936, 1978, 2444, 2547
NsiI ATGCAT 1 cut(s) 970
NspI RCATGY 2 cut(s) 1157, 3074
NspV TTCGAA 3 cut(s) 617, 2668, 3337
PaeR7I CTCGAG 1 cut(s) 3013
PagI TCATGA 1 cut(s) 165
PaqCI CACCTGC 1 cut(s) 2285
PceI AGGCCT 1 cut(s) 1221
PciI ACATGT 1 cut(s) 3070
PciSI GCTCTTC 2 cut(s) 1405, 2025
PcsI WCGNNNNNNNCGW 3 cut(s) 207, 390, 637
PctI GAATGC 2 cut(s) 809, 2664
PdmI GAANNNNTTC 2 cut(s) 3315, 3333
PfeI GAWTC 5 cut(s) 1143, 2924, 2986, 3210, 3329
Ple19I CGATCG 1 cut(s) 141
PleI GAGTC 8 cut(s) 1600, 1825, 2201, 2939, 2957, 3005, 3218, 3586
PpsI GAGTC 8 cut(s) 1600, 1825, 2201, 2939, 2957, 3005, 3218, 3586
PpuMI RGGWCCY 1 cut(s) 2732
PscI ACATGT 1 cut(s) 3070
PshAI GACNNNNGTC 3 cut(s) 173, 1630, 3228
PsiI TTATAA 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 2732
Psp6I CCWGG 1 cut(s) 1221
PspEI GGTNACC 1 cut(s) 316
PspGI CCWGG 1 cut(s) 1221
PspN4I GGNNCC 8 cut(s) 685, 859, 893, 2003, 2104, 2392, 2733, 2940
PspPI GGNCC 4 cut(s) 892, 2220, 2322, 2732
PspPPI RGGWCCY 1 cut(s) 2732
PspXI VCTCGAGB 1 cut(s) 3013
PsrI GAACNNNNNNTAC 2 cut(s) 3576, 3608
PstNI CAGNNNCTG 1 cut(s) 2117
PsuI RGATCY 3 cut(s) 857, 1131, 1753
PvuI CGATCG 1 cut(s) 141
PvuII CAGCTG 1 cut(s) 2117
RsaI GTAC 9 cut(s) 263, 277, 356, 854, 1954, 2151, 2751, 3237, 3594
RsaNI GTAC 9 cut(s) 262, 276, 355, 853, 1953, 2150, 2750, 3236, 3593
RseI CAYNNNNRTG 1 cut(s) 3512
SalI GTCGAC 1 cut(s) 3184
SapI GCTCTTC 2 cut(s) 1405, 2025
Sau96I GGNCC 4 cut(s) 892, 2220, 2322, 2732
SchI GAGTC 8 cut(s) 1601, 1826, 2201, 2939, 2957, 3005, 3218, 3587
ScrFI CCNGG 4 cut(s) 215, 322, 1223, 1891
SduI GDGCHC 5 cut(s) 598, 1467, 1582, 2199, 2349
SfcI CTRYAG 3 cut(s) 663, 1647, 1787
Sfr274I CTCGAG 1 cut(s) 3013
SfuI TTCGAA 3 cut(s) 617, 2668, 3337
SinI GGWCC 2 cut(s) 2322, 2732
SlaI CTCGAG 1 cut(s) 3013
SmiMI CAYNNNNRTG 1 cut(s) 3512
SmlI CTYRAG 3 cut(s) 3013, 3127, 3438
SmoI CTYRAG 3 cut(s) 3013, 3127, 3438
SpeI ACTAGT 1 cut(s) 1777
SseBI AGGCCT 1 cut(s) 1221
SsiI CCGC 5 cut(s) 576, 633, 650, 2186, 3649
SspI AATATT 1 cut(s) 2979
SspMI CTAG 9 cut(s) 941, 1116, 1527, 1548, 1778, 1829, 1964, 2928, 3534
StuI AGGCCT 1 cut(s) 1221
StyD4I CCNGG 4 cut(s) 213, 320, 1221, 1889
StyI CCWWGG 3 cut(s) 462, 1416, 2613
TaiI ACGT 2 cut(s) 108, 643
TaqII GACCGA 1 cut(s) 2310
TatI WGTACW 5 cut(s) 261, 354, 2149, 3235, 3592
TfiI GAWTC 5 cut(s) 1143, 2924, 2986, 3210, 3329
TscAI CASTG 7 cut(s) 117, 238, 1704, 2274, 3181, 3195, 3438
TseFI GTSAC 6 cut(s) 316, 437, 936, 1978, 2444, 2547
Tsp45I GTSAC 6 cut(s) 316, 437, 936, 1978, 2444, 2547
TspGWI ACGGA 3 cut(s) 852, 870, 945
TspRI CASTG 7 cut(s) 117, 238, 1704, 2274, 3181, 3195, 3438
VneI GTGCAC 2 cut(s) 594, 2345
VpaK11BI GGWCC 2 cut(s) 2322, 2732
XbaI TCTAGA 1 cut(s) 1828
XceI RCATGY 2 cut(s) 1157, 3074
XcmI CCANNNNNNNNNTGG 1 cut(s) 2056
XhoI CTCGAG 1 cut(s) 3013
XmiI GTMKAC 1 cut(s) 3185
XmnI GAANNNNTTC 2 cut(s) 3315, 3333
XspI CTAG 9 cut(s) 941, 1116, 1527, 1548, 1778, 1829, 1964, 2928, 3534
ZraI GACGTC 1 cut(s) 106
Zsp2I ATGCAT 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.