FvH4_3g06540

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001944
Physical Location & Seq
Forward (+)
3764660 .. 3770214
5555 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06540.t1

Sequence Viewer

Length: 3816 bp
ATGAAAAGAGAAAAAGGAAAAGATGGAGATGGCCATGGAGATGGAAAAGCCAAAGCTAACGATGTAGAGAAGCAGAAGGTCCCGTTGTTCAAGCTGTTTCAGTTTGCAGACCAATATGACGTTGCGTTGATGTTCGTCGGGAGCGTCTCTGCCATCGGAAACGGGCTGTCGCAGCCGTTCATGACTCTCATCTTCGGCAGTCTTATCAATACTTTCGGAGCTACCGACCGTGCACATATCGTCCCCATGATTTCTAAGGTGGCCTTAAAGTTCGTATACTTGGGCATTGGCACTGGAATTGCTGCATTTCTACAGGTCTCATGTTGGATGGTAACTGGCGAAAGACAGGCCACTCGAATTCGGAGCATGTACTTGAAAGCAATTGTAAGACAGGACATTGGATTTTTTGACACCGAAACAAACACCGGAGAGATCATTGGCAGAATGTCAGGGGACACAATACTCATTCAAGATGCAATGGGAGAAAAGGTGGGGAAGTTCATTCAACTCCTTTCGACCTTTGTTGGTGGTTTTATAATTGCTTTCGTCAAAGGATGGCGTCTCACCCTGGTATTATTGGCATGCATTCCCGCTATTGTCTTTGCTGGTGGAATCATGGCCATAATTGTTTCTAAAATGTCAACTCGTGGTCAACAAGCTTATGCAGAAGCTGGCACTATAGTAGAACAGACAGTTGGAGCCATTCGAACAGTTGCATCTTTTACTGGAGAGAAGCAAGCGATAGAGAGATACAACAAGAAGCTAAAGATTGCCTACAATAGTACAGTTCATCAAGGGCTAGCCACAGGGATTGGACTGGGTACATTCTTACTTATTGTCTTTGGCACTTATGGACTTGCCATATGGTATGGGTCCAAAATGATCATCGAAAAAGGATACAACGGAGGTCAAGTTATCAACGTTATATTTGCAATAATGACTGGTGGAATGTGTCTGGGGCAGACATCGCCGTCTTTGAATGCATTTGCAGCAGGAACAGCTGCTGCATACAAAATGTTTGAGACCATCAACCGAACACCGAAGATTGATCCTTATGATAATTCTGGTGTTGTATTGGACGATATTAAAGGAGAGGTGGAGCTCAAAGATGTGTATTTTAGGTATCCTGCTAGGCCAGATGTGCAAATATTTGCTGGATTCTCGTTGCATGTTCCGAGTGGCACAACGGCTGCTTTGGTTGGGCAGAGTGGAAGTGGAAAATCTACAGTCATAGGTTTGGTAGAGAGGTTTTATGATCCAGAAGCTGGTGAGGTGCTTATAGATGGTGTCAATTTGAAGAAGTTGCATCTTAGATCGATAAGAGAGAAGATTGGGCTTGTAAGTCAAGAGCCGAATCTTTTCACTACAACATTAAGGGAGAACATAGCATATGGTAAGGAAAATGCAACTCAAGAAGAGATCAGAAGAGCAATTGAACTCGCAAATGCAGCAAAGTTTATTGACAAGTTGCCTCAGGGGCTCGACACAATGGTAGGTGAGCATGGAACATCGTTATCGGGTGGACAAAAGCAAAGAATTGCGATTGCACGAGCAATTTTGAAGAACCCTAGAATTCTACTTCTAGATGAAGCAACAAGCGCCTTAGATGCTGAGTCTGAGCGAATTGTTCAAGATGCATTGGTGAGATTGATGTCAGATAGGACGACTATAGTTGTTGCACATCGCTTAACAACAATCAGGAATGCTGATGCAATAGCTGTGGTGCATAAAGGAAAAATTGTTGAATTAGGAACTCATGATGAATTAACCAGAAATCCAAAAGGAGCTTACAGCCAGTTAATTCGCCTGCAAGAAGGAGCTAAAGAAAAAGATGCGGAGCCTTCTGATCCAGATAATGTTATGGATATAGATAAGCCAATGAATAGGTCTGAAAGCATAAGACTTTCAACAACAAGATCTTTAAGCAAAAGTTCATCAGGTAGTCGGCGCTCATTCACTATCACTAGCTTTGGTGTTCCTGCTCCAGTTAATTGTGAAGTGCAAGTAGAAGAAGAAGATGAGGAGAACATTGCAAAAACTAAGGTTGATCCGGAGCAACGCAAAAAAGTTTCTATCAAAAGGTTGGCCTACTTGAATAAACCAGAGCTTCCAGTTTTGCTGCTTGGATCTATTGCTGCAGCAGGTCATGGAGTGATTTTTCCCATCTTTGGTTTACTGCTCTCAAAAGCCATTAAGATGTTTTATGAACCGCATGATAAGCTACAAAAAGACTCCAGTGTTTGGGCTGGAGTGTATGTTGGTATAGGTTTGTTTGGGTTTGCAATTATCCCAGGGCAAAACTTCTTCTTTGGAATTGCAGGTGGGAAATTGATAGAGCGAATTCGTGCCTTGACATTCCAGAAGGTTGTGCACCAACAAATTAGCTGGTTTGATGATCCTGCAAATTCAAGTGGTGCTATTGGTGCAAGGCTGTCTTCTGATGCTTCAACCGTGAAGGCCCTTGTTGGCGATGCATTAGCTTTGATTGTGCAGAATATAGCAACAATCATAGCAGGGTTAACTATAGGTTTCACAGCTAACTGGAGACTAGCGCTTATAATTATAGCTGCGTCGCCGGTGTTGATTGGACAAGGAATACTTCAAACAAATTACCTTAAAGGGTTTAGTGCTGATGCCAAGCTTATGTATGAAGAAGCAAGTCAAGTGGCAAATGATGCAATTGGTAGCATCCGAACTGTTGCATCATTTGGTTCTGAAAAGAAGGTAATGGATGCGTATGAGAAGAAGTGTGAAGGTCCAATGAAACAAGGAGTACGATTGGGAGTTGTAAGTGGTATAGGCTTTGGAGCTTCGTTTGTAGTAATGTTCTCCATAAATGCAATGATTTTCTATGTGGGAGCTCGTTTAGTGAAAAGCGGGCATGCCACTTTTGAACAAGTTTTCAAGGTTTTCTTTGCTCTAACAATTTCAGCGGTAGGGGTCTCACAATCTACTGGCATGGCTCCTGACTCCAACAAAGCTAAGGACTCAGCAGCTTCAATATTTCAAATTCTTGATAGCAAGCCTAAGATCGATTCAAGCAGCAATGCAGGCATAACACTACCGAGCATTACTGGAGAAATTGATGTTGAACATGTTAGTTTCAGATACCCCACAAGGCCAGATGTGCAGATATTTAGGGACATTTGTTTGAAATTCCCTCCTGGAAAGACTGTTGCTTTGGTTGGAGTGAGTGGCAGTGGCAAATCAACAATAATAGGCCTTATAGAGCGATTCTATGATCCCGATACAGGTCGCATACTATTAGATGGTGTTGAAATCCACAAGTTCAAAATAAACTGGTTGAGGCAACAAATTGGCTTGGTTGGTCAAGAACCTATTCTCTTTAATGAATCAATTCGTACCAACATTGCCTATGGCAAGCAAGGAAATGCAACCGAAGAAGAGATCATAGCAGCAACAACAGCATCAAATGCGCACAATTTCATATCTTCATTGCCTCAAGGATATGACACGTCTGTAGGGGAACGAGGTGTTCAATTGTCAGGTGGACAAAAACAAAGAATAGCCATTGCAAGGGCTATACTAAAGGACCCAAAAATTCTTTTGCTTGACGAAGCTACCAGTGCACTAGACACAGAGTCAGAACGCATAGTGCAACATGCTTTGGACAAAGTTATGGTGAATAGAACAACTATTGTTGTGGCTCACCGCCTGACCACGATCAAAGGGGCAGATGTAATCGCTGTGGTGAAGAATGGTGTGATTGCTGAGAAGGGAAGCCATGATATTCTAATGAAGATTACTGATGGGGCTTATGCATCATTGGTGGCACTTCATGCTAGCGCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1272

Amino Acids

137.13

Weight (kDa)

8.96

Isoelectric Point (pI)

31.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 45 - 315 3.3e-62 ABC transporter transmembrane region
ABC_tran PF00005 386 - 533 1.6e-32 ABC transporter
ABC_membrane PF00664 707 - 978 5.1e-57 ABC transporter transmembrane region
ABC_tran PF00005 1047 - 1196 3.1e-32 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 536, 2558
AarI CACCTGC 1 cut(s) 2309
AasI GACNNNNNNGTC 1 cut(s) 970
Acc16I TGCGCA 1 cut(s) 3438
Acc36I ACCTGC 2 cut(s) 2132, 2309
AccB7I CCANNNNNTGG 2 cut(s) 1265, 2241
AccI GTMKAC 1 cut(s) 276
AccIII TCCGGA 1 cut(s) 2050
AciI CCGC 6 cut(s) 591, 1835, 2210, 2877, 2933, 3673
AclI AACGTT 1 cut(s) 921
AclWI GGATC 7 cut(s) 1043, 1250, 1841, 2042, 2134, 2390, 3236
AcoI YGGCCR 2 cut(s) 31, 618
AcsI RAATTY 7 cut(s) 357, 1572, 2340, 2404, 3009, 3155, 3561
AcyI GRCGYC 1 cut(s) 559
AfaI GTAC 5 cut(s) 371, 784, 823, 2775, 3364
AfeI AGCGCT 1 cut(s) 2553
AfiI CCNNNNNNNGG 5 cut(s) 1265, 2168, 2241, 3251, 3537
AflIII ACRYGT 2 cut(s) 3094, 3474
AhdI GACNNNNNGTC 1 cut(s) 3601
AjiI CACGTC 1 cut(s) 3477
AjnI CCWGG 3 cut(s) 567, 2290, 3163
AjuI GAANNNNNNNTTGG 2 cut(s) 678, 710
Alw21I GWGCWC 5 cut(s) 235, 1104, 2373, 2863, 3592
Alw26I GTCTC 6 cut(s) 151, 322, 566, 1016, 2539, 2947
Alw44I GTGCAC 3 cut(s) 231, 2369, 3588
AlwI GGATC 7 cut(s) 1043, 1250, 1841, 2042, 2134, 2390, 3236
AlwNI CAGNNNCTG 3 cut(s) 671, 1004, 1265
Aor13HI TCCGGA 1 cut(s) 2050
Aor51HI AGCGCT 1 cut(s) 2553
AoxI GGCC 9 cut(s) 31, 261, 348, 618, 1133, 2085, 2457, 3119, 3220
ApaLI GTGCAC 3 cut(s) 231, 2369, 3588
ApoI RAATTY 7 cut(s) 357, 1572, 2340, 2404, 3009, 3155, 3561
ArsI GACNNNNNNTTYG 2 cut(s) 3163, 3195
Asp700I GAANNNNTTC 3 cut(s) 1358, 3339, 3357
AspLEI GCGC 5 cut(s) 1601, 1950, 2554, 3439, 3809
AspS9I GGNCC 5 cut(s) 79, 873, 2458, 2756, 3553
AsuHPI GGTGA 7 cut(s) 556, 1280, 1508, 1654, 3655, 3662, 3724
AsuII TTCGAA 1 cut(s) 706
AsuNHI GCTAGC 2 cut(s) 799, 3803
AvaII GGWCC 4 cut(s) 79, 873, 2756, 3553
AxyI CCTNAGG 1 cut(s) 1473
BaeGI GKGCMC 3 cut(s) 235, 2373, 3592
BalI TGGCCA 2 cut(s) 33, 620
BanII GRGCYC 3 cut(s) 1104, 1482, 2863
BauI CACGAG 2 cut(s) 645, 1548
BbsI GAAGAC 1 cut(s) 2427
Bbv12I GWGCWC 5 cut(s) 235, 1104, 2373, 2863, 3592
BceAI ACGGC 3 cut(s) 160, 955, 1203
BciT130I CCWGG 3 cut(s) 569, 2292, 3165
BciVI GTATCC 2 cut(s) 890, 1134
BclI TGATCA 1 cut(s) 882
BcoDI GTCTC 6 cut(s) 151, 322, 566, 1016, 2539, 2947
BfaI CTAG 8 cut(s) 800, 1131, 1569, 1583, 1965, 2549, 3593, 3804
BfmI CTRYAG 7 cut(s) 311, 678, 1224, 1668, 2136, 2523, 3480
BfoI RGCGCY 3 cut(s) 1602, 1951, 2555
BfuAI ACCTGC 2 cut(s) 2132, 2309
BfuI GTATCC 2 cut(s) 890, 1134
BglI GCCNNNNNGGC 1 cut(s) 1477
BglII AGATCT 1 cut(s) 1916
Bme1390I CCNGG 3 cut(s) 569, 2292, 3165
Bme18I GGWCC 4 cut(s) 79, 873, 2756, 3553
BmeRI GACNNNNNGTC 1 cut(s) 3601
BmgBI CACGTC 1 cut(s) 3477
BmgT120I GGNCC 5 cut(s) 79, 873, 2458, 2756, 3553
BmiI GGNNCC 6 cut(s) 81, 700, 874, 1839, 2964, 3555
BmrFI CCNGG 3 cut(s) 569, 2292, 3165
BmrI ACTGGG 1 cut(s) 827
BmtI GCTAGC 2 cut(s) 803, 3807
BmuI ACTGGG 1 cut(s) 827
BpiI GAAGAC 1 cut(s) 2427
BpmI CTGGAG 6 cut(s) 747, 1968, 2218, 2268, 2563, 3096
Bpu10I CCTNAGC 1 cut(s) 2982
Bpu14I TTCGAA 1 cut(s) 706
BpuEI CTTGAG 2 cut(s) 1395, 3447
Bsa29I ATCGAT 2 cut(s) 1316, 3033
BsaHI GRCGYC 1 cut(s) 559
BsaI GGTCTC 3 cut(s) 322, 1016, 2947
BsaJI CCNNGG 4 cut(s) 34, 567, 2290, 2291
BsaWI WCCGGW 2 cut(s) 425, 2050
BsaXI ACNNNNNCTCC 6 cut(s) 474, 504, 897, 927, 2241, 2271
Bsc4I CCNNNNNNNGG 5 cut(s) 1265, 2168, 2241, 3251, 3537
Bse118I RCCGGY 1 cut(s) 2575
Bse21I CCTNAGG 1 cut(s) 1473
Bse3DI GCAATG 7 cut(s) 483, 2028, 2847, 3052, 3369, 3455, 3531
BseAI TCCGGA 1 cut(s) 2050
BseBI CCWGG 3 cut(s) 569, 2292, 3165
BseCI ATCGAT 2 cut(s) 1316, 3033
BseDI CCNNGG 4 cut(s) 34, 567, 2290, 2291
BseGI GGATG 4 cut(s) 333, 560, 2688, 2737
BseLI CCNNNNNNNGG 5 cut(s) 1265, 2168, 2241, 3251, 3537
BseMI GCAATG 7 cut(s) 483, 2028, 2847, 3052, 3369, 3455, 3531
BseMII CTCAG 5 cut(s) 1487, 1602, 1608, 3003, 3723
BseRI GAGGAG 1 cut(s) 2036
BseSI GKGCMC 3 cut(s) 235, 2373, 3592
BsgI GTGCAG 2 cut(s) 2510, 3149
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 9 cut(s) 33, 263, 350, 620, 1135, 2087, 2459, 3121, 3222
BshVI ATCGAT 2 cut(s) 1316, 3033
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 5 cut(s) 235, 1104, 2373, 2863, 3592
BsiSI CCGG 3 cut(s) 426, 2051, 2576
BslFI GGGAC 4 cut(s) 65, 227, 467, 3155
BslI CCNNNNNNNGG 5 cut(s) 1265, 2168, 2241, 3251, 3537
BsmAI GTCTC 6 cut(s) 151, 322, 566, 1016, 2539, 2947
BsmBI CGTCTC 2 cut(s) 151, 566
BsmFI GGGAC 4 cut(s) 65, 227, 467, 3155
BsmI GAATGC 3 cut(s) 585, 985, 1708
BsnI GGCC 9 cut(s) 33, 263, 350, 620, 1135, 2087, 2459, 3121, 3222
Bso31I GGTCTC 3 cut(s) 322, 1016, 2947
Bsp119I TTCGAA 1 cut(s) 706
Bsp1286I GDGCHC 6 cut(s) 235, 1104, 1482, 2373, 2863, 3592
Bsp13I TCCGGA 1 cut(s) 2050
Bsp19I CCATGG 1 cut(s) 34
BspACI CCGC 6 cut(s) 591, 1835, 2210, 2877, 2933, 3673
BspANI GGCC 9 cut(s) 33, 263, 350, 620, 1135, 2087, 2459, 3121, 3222
BspCNI CTCAG 5 cut(s) 1486, 1603, 1609, 3002, 3724
BspDI ATCGAT 2 cut(s) 1316, 3033
BspEI TCCGGA 1 cut(s) 2050
BspHI TCATGA 3 cut(s) 180, 1756, 3812
BspLI GGNNCC 6 cut(s) 81, 700, 874, 1839, 2964, 3555
BspMAI CTGCAG 1 cut(s) 2140
BspMI ACCTGC 2 cut(s) 2132, 2309
BspOI GCTAGC 2 cut(s) 803, 3807
BspPI GGATC 7 cut(s) 1043, 1250, 1841, 2042, 2134, 2390, 3236
BspQI GCTCTTC 1 cut(s) 1420
BspT104I TTCGAA 1 cut(s) 706
BspTNI GGTCTC 3 cut(s) 322, 1016, 2947
BsrDI GCAATG 7 cut(s) 483, 2028, 2847, 3052, 3369, 3455, 3531
BsrFI RCCGGY 1 cut(s) 2575
BssAI RCCGGY 1 cut(s) 2575
BssECI CCNNGG 4 cut(s) 34, 567, 2290, 2291
BssNAI GTATAC 1 cut(s) 277
BssNI GRCGYC 1 cut(s) 559
BssSI CACGAG 2 cut(s) 645, 1548
BssT1I CCWWGG 1 cut(s) 34
Bst1107I GTATAC 1 cut(s) 277
Bst2BI CACGAG 2 cut(s) 645, 1548
Bst2UI CCWGG 3 cut(s) 569, 2292, 3165
Bst4CI ACNGT 8 cut(s) 230, 694, 712, 787, 1228, 2452, 2698, 3175
Bst6I CTCTTC 3 cut(s) 1410, 1420, 3399
BstACI GRCGYC 1 cut(s) 559
BstAPI GCANNNNNTGC 3 cut(s) 2675, 3434, 3800
BstBI TTCGAA 1 cut(s) 706
BstDSI CCRYGG 1 cut(s) 34
BstF5I GGATG 4 cut(s) 333, 560, 2688, 2737
BstH2I RGCGCY 3 cut(s) 1602, 1951, 2555
BstHHI GCGC 5 cut(s) 1601, 1950, 2554, 3439, 3809
BstMAI GTCTC 6 cut(s) 151, 322, 566, 1016, 2539, 2947
BstMCI CGRYCG 1 cut(s) 229
BstNI CCWGG 3 cut(s) 569, 2292, 3165
BstNSI RCATGY 6 cut(s) 370, 585, 1172, 2885, 3098, 3626
BstSCI CCNGG 3 cut(s) 567, 2290, 3163
BstSFI CTRYAG 7 cut(s) 311, 678, 1224, 1668, 2136, 2523, 3480
BstSLI GKGCMC 3 cut(s) 235, 2373, 3592
BstV2I GAAGAC 1 cut(s) 2427
BstX2I RGATCY 2 cut(s) 1916, 2126
BstXI CCANNNNNNTGG 1 cut(s) 41
BstYI RGATCY 2 cut(s) 1916, 2126
BstZ17I GTATAC 1 cut(s) 277
Bsu15I ATCGAT 2 cut(s) 1316, 3033
Bsu36I CCTNAGG 1 cut(s) 1473
BsuI GTATCC 2 cut(s) 890, 1134
BsuRI GGCC 9 cut(s) 33, 263, 350, 620, 1135, 2087, 2459, 3121, 3222
BsuTUI ATCGAT 2 cut(s) 1316, 3033
BtgI CCRYGG 1 cut(s) 34
BtgZI GCGATG 3 cut(s) 951, 1667, 2484
BtrI CACGTC 1 cut(s) 3477
BtsCI GGATG 4 cut(s) 333, 560, 2688, 2737
BtsI GCAGTG 1 cut(s) 3205
BtsIMutI CAGTG 4 cut(s) 291, 2242, 3205, 3592
BveI ACCTGC 2 cut(s) 2132, 2309
CaiI CAGNNNCTG 3 cut(s) 671, 1004, 1265
CciI TCATGA 3 cut(s) 180, 1756, 3812
CfoI GCGC 5 cut(s) 1601, 1950, 2554, 3439, 3809
Cfr10I RCCGGY 1 cut(s) 2575
Cfr13I GGNCC 5 cut(s) 79, 873, 2458, 2756, 3553
ClaI ATCGAT 2 cut(s) 1316, 3033
CseI GACGC 3 cut(s) 133, 548, 2559
Csp6I GTAC 5 cut(s) 370, 783, 822, 2774, 3363
CspCI CAANNNNNGTGG 6 cut(s) 793, 828, 1701, 1736, 2646, 2681
CviQI GTAC 5 cut(s) 370, 783, 822, 2774, 3363
DrdI GACNNNNNNGTC 1 cut(s) 970
DriI GACNNNNNGTC 1 cut(s) 3601
DseDI GACNNNNNNGTC 1 cut(s) 970
EaeI YGGCCR 2 cut(s) 31, 618
Eam1104I CTCTTC 3 cut(s) 1410, 1420, 3399
Eam1105I GACNNNNNGTC 1 cut(s) 3601
EarI CTCTTC 3 cut(s) 1410, 1420, 3399
Ecl136II GAGCTC 2 cut(s) 1102, 2861
Eco130I CCWWGG 1 cut(s) 34
Eco147I AGGCCT 1 cut(s) 3222
Eco24I GRGCYC 3 cut(s) 1104, 1482, 2863
Eco31I GGTCTC 3 cut(s) 322, 1016, 2947
Eco47I GGWCC 4 cut(s) 79, 873, 2756, 3553
Eco47III AGCGCT 1 cut(s) 2553
Eco53kI GAGCTC 2 cut(s) 1102, 2861
Eco81I CCTNAGG 1 cut(s) 1473
EcoICRI GAGCTC 2 cut(s) 1102, 2861
EcoO109I RGGNCCY 3 cut(s) 79, 2458, 3553
EcoRI GAATTC 3 cut(s) 357, 1572, 2340
EcoRII CCWGG 3 cut(s) 567, 2290, 3163
EcoT14I CCWWGG 1 cut(s) 34
EcoT22I ATGCAT 5 cut(s) 587, 985, 1639, 2476, 3784
EcoT38I GRGCYC 3 cut(s) 1104, 1482, 2863
ErhI CCWWGG 1 cut(s) 34
Esp3I CGTCTC 2 cut(s) 151, 566
FalI AAGNNNNNCTT 8 cut(s) 248, 280, 2419, 2451, 2583, 2615, 2897, 2929
FaqI GGGAC 4 cut(s) 65, 227, 467, 3155
FauI CCCGC 2 cut(s) 598, 2870
FauNDI CATATG 2 cut(s) 863, 1390
FbaI TGATCA 1 cut(s) 882
FblI GTMKAC 1 cut(s) 276
FokI GGATG 4 cut(s) 340, 567, 2675, 2744
FriOI GRGCYC 3 cut(s) 1104, 1482, 2863
FspAI RTGCGCAY 1 cut(s) 3438
FspBI CTAG 8 cut(s) 800, 1131, 1569, 1583, 1965, 2549, 3593, 3804
FspI TGCGCA 1 cut(s) 3438
GlaI GCGC 5 cut(s) 1600, 1949, 2553, 3438, 3808
GsuI CTGGAG 6 cut(s) 747, 1968, 2218, 2268, 2563, 3096
HaeII RGCGCY 3 cut(s) 1602, 1951, 2555
HaeIII GGCC 9 cut(s) 33, 263, 350, 620, 1135, 2087, 2459, 3121, 3222
HapII CCGG 3 cut(s) 426, 2051, 2576
HgaI GACGC 3 cut(s) 133, 548, 2559
HhaI GCGC 5 cut(s) 1601, 1950, 2554, 3439, 3809
Hin1I GRCGYC 1 cut(s) 559
Hin6I GCGC 5 cut(s) 1599, 1948, 2552, 3437, 3807
HinP1I GCGC 5 cut(s) 1599, 1948, 2552, 3437, 3807
HincII GTYRAC 3 cut(s) 642, 653, 2520
HindII GTYRAC 3 cut(s) 642, 653, 2520
HindIII AAGCTT 2 cut(s) 657, 2639
HpaI GTTAAC 1 cut(s) 2520
HpaII CCGG 3 cut(s) 426, 2051, 2576
HphI GGTGA 7 cut(s) 556, 1280, 1508, 1654, 3655, 3662, 3724
Hpy99I CGWCG 2 cut(s) 140, 2575
HpyAV CCTTC 8 cut(s) 70, 1808, 1851, 2356, 2449, 2716, 2747, 3730
HpyCH4III ACNGT 8 cut(s) 230, 694, 712, 787, 1228, 2452, 2698, 3175
HpyCH4IV ACGT 3 cut(s) 120, 921, 3476
HpySE526I ACGT 3 cut(s) 120, 921, 3476
Hsp92I GRCGYC 1 cut(s) 559
HspAI GCGC 5 cut(s) 1599, 1948, 2552, 3437, 3807
Kpn2I TCCGGA 1 cut(s) 2050
Ksp22I TGATCA 1 cut(s) 882
KspAI GTTAAC 1 cut(s) 2520
LguI GCTCTTC 1 cut(s) 1420
MaeI CTAG 8 cut(s) 800, 1131, 1569, 1583, 1965, 2549, 3593, 3804
MaeII ACGT 3 cut(s) 120, 921, 3476
MaeIII GTNAC 1 cut(s) 331
MfeI CAATTG 4 cut(s) 381, 1431, 2679, 3500
MflI RGATCY 2 cut(s) 1916, 2126
MhlI GDGCHC 6 cut(s) 235, 1104, 1482, 2373, 2863, 3592
MlsI TGGCCA 2 cut(s) 33, 620
MluNI TGGCCA 2 cut(s) 33, 620
MlyI GAGTC 6 cut(s) 178, 1622, 2225, 2963, 2981, 3611
MmeI TCCRAC 4 cut(s) 305, 676, 2997, 3166
Mox20I TGGCCA 2 cut(s) 33, 620
Mph1103I ATGCAT 5 cut(s) 587, 985, 1639, 2476, 3784
MroI TCCGGA 1 cut(s) 2050
MroXI GAANNNNTTC 3 cut(s) 1358, 3339, 3357
MscI TGGCCA 2 cut(s) 33, 620
MslI CAYNNNNRTG 2 cut(s) 39, 2195
Msp20I TGGCCA 2 cut(s) 33, 620
MspA1I CMGCKG 2 cut(s) 1001, 2933
MspI CCGG 3 cut(s) 426, 2051, 2576
MspR9I CCNGG 3 cut(s) 569, 2292, 3165
MunI CAATTG 4 cut(s) 381, 1431, 2679, 3500
Mva1269I GAATGC 3 cut(s) 585, 985, 1708
MvaI CCWGG 3 cut(s) 569, 2292, 3165
NcoI CCATGG 1 cut(s) 34
NdeI CATATG 2 cut(s) 863, 1390
NheI GCTAGC 2 cut(s) 799, 3803
NlaIV GGNNCC 6 cut(s) 81, 700, 874, 1839, 2964, 3555
NsbI TGCGCA 1 cut(s) 3438
NsiI ATGCAT 5 cut(s) 587, 985, 1639, 2476, 3784
NspI RCATGY 6 cut(s) 370, 585, 1172, 2885, 3098, 3626
NspV TTCGAA 1 cut(s) 706
PaeI GCATGC 2 cut(s) 585, 2885
PagI TCATGA 3 cut(s) 180, 1756, 3812
PaqCI CACCTGC 1 cut(s) 2309
PasI CCCWGGG 1 cut(s) 2291
PceI AGGCCT 1 cut(s) 3222
PciI ACATGT 1 cut(s) 3094
PciSI GCTCTTC 1 cut(s) 1420
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 222
PctI GAATGC 3 cut(s) 585, 985, 1708
PdmI GAANNNNTTC 3 cut(s) 1358, 3339, 3357
PfeI GAWTC 6 cut(s) 612, 1158, 1354, 3035, 3234, 3353
PflMI CCANNNNNTGG 2 cut(s) 1265, 2241
PfoI TCCNGGA 1 cut(s) 3163
PleI GAGTC 6 cut(s) 178, 1621, 2225, 2963, 2981, 3610
PpsI GAGTC 6 cut(s) 178, 1621, 2225, 2963, 2981, 3610
PpuMI RGGWCCY 2 cut(s) 79, 3553
PscI ACATGT 1 cut(s) 3094
PsiI TTATAA 2 cut(s) 536, 2558
Psp124BI GAGCTC 2 cut(s) 1104, 2863
Psp1406I AACGTT 1 cut(s) 921
Psp5II RGGWCCY 2 cut(s) 79, 3553
Psp6I CCWGG 3 cut(s) 567, 2290, 3163
PspGI CCWGG 3 cut(s) 567, 2290, 3163
PspN4I GGNNCC 6 cut(s) 81, 700, 874, 1839, 2964, 3555
PspPI GGNCC 5 cut(s) 79, 873, 2458, 2756, 3553
PspPPI RGGWCCY 2 cut(s) 79, 3553
PstI CTGCAG 1 cut(s) 2140
PstNI CAGNNNCTG 3 cut(s) 671, 1004, 1265
PsuI RGATCY 2 cut(s) 1916, 2126
PvuII CAGCTG 1 cut(s) 1001
RsaI GTAC 5 cut(s) 371, 784, 823, 2775, 3364
RsaNI GTAC 5 cut(s) 370, 783, 822, 2774, 3363
RseI CAYNNNNRTG 2 cut(s) 39, 2195
SacI GAGCTC 2 cut(s) 1104, 2863
SapI GCTCTTC 1 cut(s) 1420
Sau96I GGNCC 5 cut(s) 79, 873, 2458, 2756, 3553
SchI GAGTC 6 cut(s) 178, 1622, 2225, 2963, 2981, 3611
ScrFI CCNGG 3 cut(s) 569, 2292, 3165
SduI GDGCHC 6 cut(s) 235, 1104, 1482, 2373, 2863, 3592
SfcI CTRYAG 7 cut(s) 311, 678, 1224, 1668, 2136, 2523, 3480
SfuI TTCGAA 1 cut(s) 706
SgrAI CRCCGGYG 1 cut(s) 2575
SinI GGWCC 4 cut(s) 79, 873, 2756, 3553
SmiMI CAYNNNNRTG 2 cut(s) 39, 2195
SmlI CTYRAG 2 cut(s) 1410, 3462
SmoI CTYRAG 2 cut(s) 1410, 3462
SphI GCATGC 2 cut(s) 585, 2885
SseBI AGGCCT 1 cut(s) 3222
SsiI CCGC 6 cut(s) 591, 1835, 2210, 2877, 2933, 3673
SspI AATATT 2 cut(s) 1149, 3003
SspMI CTAG 8 cut(s) 800, 1131, 1569, 1583, 1965, 2549, 3593, 3804
SstI GAGCTC 2 cut(s) 1104, 2863
StuI AGGCCT 1 cut(s) 3222
StyD4I CCNGG 3 cut(s) 567, 2290, 3163
StyI CCWWGG 1 cut(s) 34
TaaI ACNGT 8 cut(s) 230, 694, 712, 787, 1228, 2452, 2698, 3175
TaiI ACGT 3 cut(s) 123, 924, 3479
TaqI TCGA 7 cut(s) 355, 515, 706, 888, 1316, 1482, 3033
TatI WGTACW 2 cut(s) 369, 782
TfiI GAWTC 6 cut(s) 612, 1158, 1354, 3035, 3234, 3353
TscAI CASTG 4 cut(s) 298, 2242, 3205, 3592
TspGWI ACGGA 1 cut(s) 918
TspRI CASTG 4 cut(s) 298, 2242, 3205, 3592
Van91I CCANNNNNTGG 2 cut(s) 1265, 2241
VneI GTGCAC 3 cut(s) 231, 2369, 3588
VpaK11BI GGWCC 4 cut(s) 79, 873, 2756, 3553
XapI RAATTY 7 cut(s) 357, 1572, 2340, 2404, 3009, 3155, 3561
XbaI TCTAGA 1 cut(s) 1582
XceI RCATGY 6 cut(s) 370, 585, 1172, 2885, 3098, 3626
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 3 cut(s) 1358, 3339, 3357
XspI CTAG 8 cut(s) 800, 1131, 1569, 1583, 1965, 2549, 3593, 3804
Zsp2I ATGCAT 5 cut(s) 587, 985, 1639, 2476, 3784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.