Rw5G008520

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001944
Physical Location & Seq
Reverse (-)
9197579 .. 9203006
5428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G008520.1

Sequence Viewer

Length: 3801 bp
ATGGAAGGAGGAGGAGGAGATGGAGATGGAAGAGCAAAAGTTAACGATCCAGACAAGCAGAAGGTCCCGTTCTACAAGCTGTTTCTGTTCGCAGACCAATATGACGTGGGGTTGATGATCGTCGGGAGTATCTGTGCCATCGGAAACGGGCTGTCGCAGCCGTTCATGACGCTCATCTTCGGCAGTCTTATCAATACTTTCGGACATACTGACCGGGCTCATATCGTCCCCATGATTTCTAAGGTGTCTCTAAAATTTGTGTACTTGGGCATTGGTACTGGCATTGCTGCATTTCTACAGGTATCATGTTGGATGGTAACTGGGGAAAGACAGGCCACTCGAATTCGAAGCTTGTACTTGAAAGCAATTTTAAGACAGGACATTGGATATTTTGACACCGAAACTAACACTGGAGAGATCATTGGCAGAATGTCAGGGGACACAATTCTCATTCAAGATGCCATGGGAGAAAAGGTGGGGAAGTTCATTCAACTCCTTTCAACTTTTGTTGGCGGTTTTGTAATTGCTTTGGTCAAAGGATGGCTTCTTACCCTGGTGTTACTGTCATGTATTCCCGCTATTGTCTTTGCTGGTGGAATCATGGCCATTATTGTTTCCAAAATGTCAACACGTGGACAGCGTGCTTATGCGGAAGCTGGCAGTATAGTAGAACAAACAGTTGGAGCCATTCGAACAGTTGCATCTTTTACCGGAGAGAAGCAAGCGATAGAAAAATATAACAAGAAGCTAAAGGTTGCCTACAATAGTACAGTTCAACAAGGACTTGCCACAGGAGTTGGACTTGGTACGTTCATACTTATTGTCTTTGGCACTTATGGACTTGCCATATGGTATGGATCCAAAATGATCATCGAAAAAGGATATAATGGAGGCCAAGTCATCAATGTTATATTTGCAATAATGACGGGTGGAATGTGCCTGGGCCAGACATCACCGTCTTTGAATGCATTTGCAGCAGGGACAGCTGCTGCATACAAAATGCTCGAGACTATCAAACGAACGCCGAAGATTGATCCTTATGATACCTCTGGAGTTGTATTGGACGATATTAAAGGAGAGGTGGAGCTCAAAGACGTCCATTTCAGGTACCCTGCCAGGCCGGATGTGCAGATTTTTGCGGGATTCTCGTTGCATGTTCCGAGTGGCACAACGACTGCTTTGGTTGGGCAGAGTGGAAGTGGGAAGTCAACAGTGATAGGCTTGGTAGAGAGGTTTTATGATCCAGAAGCTGGTGAAGTACTTATAGATGGTGTCAATTTGAAGAAGTTGCATCTTAGATCGATAAGGGAGAAGATTGGGCTTGTAAGTCAAGAGCCAAATCTTTTTACTACAACAATAAGGGAGAACATAGCATATGGGAAGGAAAATGCAACTCAAGAAGAGATTAGAAGAGCTATTGAGCTCGCAAATGCAGCAAAGTTTATTGACAAGTTGCCTCAGGGGCTCGACACAATGGTAGGTGAGCATGGAACATCGCTATCGGGTGGACAAAAGCAAAGAATTGCAATTGCAAGAGCCATTTTAAAGAACCCTAGAATTCTACTTCTGGACGAAGCAACAAGCGCCTTGGATGCTGAGTCGGAGCGAATTGTTCAAGATGCACTGGTGAGATTGATGTCAAATAGAACGACTATAGTTGTTGCACATCGCTTAACAACGATTAGGAATGCTGATGCAATAGCTGTGGTGCATAAAGGGAAAATTGTTGAAATAGGAACTCATGATGACTTGACCCAAAACCCAGAAGGAGCGTACAGCCAACTAATCCGCCTGCAAGAAAGAGCTAAAGAAAAAGGTGCAGAGCCCTCTGATCCAGATAATGGTTTGGATATAGATAGGACAATGAGTAGATCTGGGAGCCAGAGACTTTCAGCAGTGAGATCTTTAAGCAAAGGTTCATCAGGTAGTCGGCGCTCATTCACTATTACTAGCTTTGGTGTTCCTGCTCCAGTTAATTGTGAAGTACAAGTAGAAGAAGAAGATGAGGAGAACCGTGTGAAAACGAAGGTTGATCCGGAGCAACGCAAAAAAGTTTCCATCAAAAGGCTGGCCTATTTGAATAAACCTGAGCTTCCAGTTTTGCTGCTTGGATCTATTGCTGCAGCAGGTCATGGAGTGATTTTTCCTGTCTTCGGCTTACTACTCTCAAAAGCCATCAAGATGTTCTATGAGCCGCATAATGAGCTGCGAAAAGACTCCAAAGTTTGGGCAGGAGTGTATGTTGGTATAGGTTTCTTTGGGTTGTTTGTTATTCCAGGGCAAAATTTCTTCTTTGGAATTGCAGGTGGGAAGCTGATAGAGCGAATTCGTGCCTTGACATTCCAAAAAGTTGTACACCAGCAAATTAGTTGGTTTGATGATCCTGCAAATTCAAGTGGTGCTATTGGTGCAAGGTTGTCTTCTGATGCTTCAACTGTGAAGGCTCTTGTTGGTGACGCATTAGCCTTGATTGCTCAGAACATAGCAACAATCATAGCAGGACTGATTATAGGTTTCGTAGCTAACTGGAGACTAGCGCTTATAATTATAGCTGTGTCGCCATTGTTGATTGGGCAAGGAATACTCCAAACAAAATTTCTTAAAGGGTTTAGTGCCGATGCCAAGGTTATGTATGAAGAAGCAAGTCAAGTGGCAAATGATGCAATTGGTAGCATTCGAACTGTTGCATCATTTTGTTCTGAAAAGAAGGTGATGGATACGTATGAGAAGAAGTGTGAAGGTCCAATGAAACAAGGAGTACGATTGGGAGTAGTAAGTGGTACAGGTTTTGGCGCTTCATTTGTAGTAATGTTCTTCACAAATGCATTAATTTTCTATGTTGGAGCTCATCTTGTGAAAAATGGGCAAGCCACATTTGAACAAGTATTCAGGGTGTTCTTTGCTCTAACTATTTCAGCGGTGGGGGTTTCTCAATCTACTGGCATGGCTCCTGACTCCAATAAAGCCAAGGACTCAGCAGCTTCAATATTTCAAATTCTTGATAGCAAGCCTAAGATCGATTCAAGCAGCGATGATGGCATAACACTACCGCGCATTACTGGAGAAATTGATCTTGACCATGTCAGCTTCAAATACCCCACAAGACCAGATGTGCAGATATTCAGGGACATTTGTTTGAAAATCCCTTCTGGAAAGACTGTTGCTTTGGTTGGAGAGAGTGGCAGTGGGAAATCGACAGTAATAGGCCTTATAGAGCGATTCTATGACCCCGATACAGGTCGCGTACTGTTAGACGGAGTGGAAATCCACAAGTTCAAGCTAAACTGGTTGAGGCAACAGATTGGTTTGGTTGGTCAAGAACCTATTCTCTTTAATGAGTCAATTCGTACCAACATTGCCTATGGCAAGCAGGGAGATGCAACAGAAGAAGAGATCATAGCAGCAACAACTGCATCAAATGCGCACAATTTCATATCTTCATTGCCTCAAGGATATGACACCTCAGTGGGGGAACGAGGTGTTCAGTTATCAGGCGGACAAAAGCAAAGAATAGCCATTGCAAGGGCTATACTAAAGGACCCAAAAATCCTTTTGCTTGACGAAGCTACCAGTGCACTAGACGCAGAATCAGAACGTATAGTACAAGATGCTTTGGACAGAGTTATGGTGAATAGGACAACTGTTGTTGTTGCTCACCGCCTCACCACGATCAAAGGAGCAGATATAATCGCTGTGGTGAAGAATGGTGTGATTGCCGAGAAGGGAAACCATGATTTTCTGATGAAGATCACTGATGGGGCTTATGCATCTTTGGTGGCACTTCACGCTAGTCCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1266

Amino Acids

136.62

Weight (kDa)

8.78

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 39 - 310 3e-61 ABC transporter transmembrane region
ABC_tran PF00005 381 - 528 3.8e-33 ABC transporter
ABC_membrane PF00664 702 - 973 9.5e-60 ABC transporter transmembrane region
ABC_tran PF00005 1042 - 1191 1.7e-32 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2543
AarI CACCTGC 1 cut(s) 2294
AasI GACNNNNNNGTC 1 cut(s) 955
AatII GACGTC 1 cut(s) 1098
Acc16I TGCGCA 1 cut(s) 3423
Acc36I ACCTGC 2 cut(s) 2117, 2294
Acc65I GGTACC 1 cut(s) 1107
AccB1I GGYRCC 1 cut(s) 1107
AccB7I CCANNNNNTGG 3 cut(s) 1250, 1841, 2226
AccII CGCG 2 cut(s) 3052, 3243
AccIII TCCGGA 1 cut(s) 2035
AclWI GGATC 9 cut(s) 41, 852, 865, 1028, 1235, 1826, 2027, 2119, 2375
AcoI YGGCCR 1 cut(s) 603
AcsI RAATTY 8 cut(s) 254, 342, 1557, 2284, 2325, 2389, 2594, 2994
AcvI CACGTG 1 cut(s) 632
AcyI GRCGYC 1 cut(s) 1095
AfeI AGCGCT 1 cut(s) 2538
AfiI CCNNNNNNNGG 8 cut(s) 1250, 1841, 2064, 2153, 2226, 3236, 3468, 3522
AflIII ACRYGT 1 cut(s) 629
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 4 cut(s) 552, 939, 1115, 2275
AjuI GAANNNNNNNTTGG 4 cut(s) 663, 695, 2580, 2612
AleI CACNNNNGTG 1 cut(s) 3464
Alw21I GWGCWC 4 cut(s) 1089, 1425, 2848, 3577
Alw26I GTCTC 4 cut(s) 252, 1001, 1879, 2524
Alw44I GTGCAC 1 cut(s) 3573
AlwI GGATC 9 cut(s) 41, 852, 865, 1028, 1235, 1826, 2027, 2119, 2375
AlwNI CAGNNNCTG 2 cut(s) 989, 1250
Ama87I CYCGRG 1 cut(s) 1004
Aor13HI TCCGGA 1 cut(s) 2035
Aor51HI AGCGCT 1 cut(s) 2538
AoxI GGCC 7 cut(s) 333, 603, 892, 943, 1118, 2070, 3205
ApaLI GTGCAC 1 cut(s) 3573
ApoI RAATTY 8 cut(s) 254, 342, 1557, 2284, 2325, 2389, 2594, 2994
ArsI GACNNNNNNTTYG 2 cut(s) 3148, 3180
AseI ATTAAT 1 cut(s) 2828
Asp700I GAANNNNTTC 1 cut(s) 3324
Asp718I GGTACC 1 cut(s) 1107
AspLEI GCGC 6 cut(s) 1586, 1935, 2539, 2795, 3054, 3424
AspS9I GGNCC 4 cut(s) 64, 943, 2741, 3538
AsuC2I CCSGG 1 cut(s) 215
AsuII TTCGAA 3 cut(s) 346, 691, 2677
AvaI CYCGRG 1 cut(s) 1004
AvaII GGWCC 3 cut(s) 64, 2741, 3538
AxyI CCTNAGG 1 cut(s) 1458
BaeGI GKGCMC 1 cut(s) 3577
BalI TGGCCA 1 cut(s) 605
BamHI GGATCC 1 cut(s) 857
BanI GGYRCC 1 cut(s) 1107
BanII GRGCYC 6 cut(s) 220, 1089, 1425, 1467, 1827, 2848
BbrPI CACGTG 1 cut(s) 632
BbsI GAAGAC 2 cut(s) 2143, 2412
Bbv12I GWGCWC 4 cut(s) 1089, 1425, 2848, 3577
BceAI ACGGC 1 cut(s) 145
BciT130I CCWGG 4 cut(s) 554, 941, 1117, 2277
BciVI GTATCC 1 cut(s) 2710
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 4 cut(s) 252, 1001, 1879, 2524
BfaI CTAG 5 cut(s) 1554, 1950, 2534, 3578, 3789
BfmI CTRYAG 3 cut(s) 296, 1653, 2121
BfoI RGCGCY 4 cut(s) 1587, 1936, 2540, 2796
BfuAI ACCTGC 2 cut(s) 2117, 2294
BfuI GTATCC 1 cut(s) 2710
BglI GCCNNNNNGGC 1 cut(s) 1462
BglII AGATCT 2 cut(s) 1871, 1901
BmcAI AGTACT 1 cut(s) 1260
Bme1390I CCNGG 5 cut(s) 215, 554, 941, 1117, 2277
Bme18I GGWCC 3 cut(s) 64, 2741, 3538
BmeT110I CYCGRG 1 cut(s) 1004
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 4 cut(s) 64, 943, 2741, 3538
BmiI GGNNCC 7 cut(s) 66, 685, 859, 1109, 1880, 2949, 3540
BmrFI CCNGG 5 cut(s) 215, 554, 941, 1117, 2277
BmrI ACTGGG 1 cut(s) 330
BmuI ACTGGG 1 cut(s) 330
BpiI GAAGAC 2 cut(s) 2143, 2412
BpmI CTGGAG 5 cut(s) 432, 1071, 1953, 2548, 3081
Bpu10I CCTNAGC 1 cut(s) 2088
Bpu14I TTCGAA 3 cut(s) 346, 691, 2677
BpuEI CTTGAG 2 cut(s) 1380, 3432
BpuMI CCSGG 1 cut(s) 215
Bsa29I ATCGAT 2 cut(s) 1301, 3018
BsaAI YACGTR 2 cut(s) 632, 2721
BsaBI GATNNNNATC 1 cut(s) 3746
BsaHI GRCGYC 1 cut(s) 1095
BsaJI CCNNGG 7 cut(s) 462, 552, 940, 1587, 2276, 2622, 2967
BsaWI WCCGGW 2 cut(s) 710, 2035
BsaXI ACNNNNNCTCC 8 cut(s) 118, 148, 459, 489, 882, 912, 2226, 2256
Bsc4I CCNNNNNNNGG 8 cut(s) 1250, 1841, 2064, 2153, 2226, 3236, 3468, 3522
Bse21I CCTNAGG 1 cut(s) 1458
Bse3DI GCAATG 4 cut(s) 282, 3354, 3440, 3516
Bse8I GATNNNNATC 1 cut(s) 3746
BseAI TCCGGA 1 cut(s) 2035
BseBI CCWGG 4 cut(s) 554, 941, 1117, 2277
BseCI ATCGAT 2 cut(s) 1301, 3018
BseDI CCNNGG 7 cut(s) 462, 552, 940, 1587, 2276, 2622, 2967
BseGI GGATG 4 cut(s) 318, 545, 1129, 1597
BseJI GATNNNNATC 1 cut(s) 3746
BseLI CCNNNNNNNGG 8 cut(s) 1250, 1841, 2064, 2153, 2226, 3236, 3468, 3522
BseMI GCAATG 4 cut(s) 282, 3354, 3440, 3516
BseMII CTCAG 6 cut(s) 1472, 1587, 2079, 2489, 2988, 3477
BseRI GAGGAG 4 cut(s) 24, 27, 30, 2021
BseSI GKGCMC 1 cut(s) 3577
BsgI GTGCAG 3 cut(s) 1148, 1839, 3134
Bsh1236I CGCG 2 cut(s) 3052, 3243
BshFI GGCC 7 cut(s) 335, 605, 894, 945, 1120, 2072, 3207
BshNI GGYRCC 1 cut(s) 1107
BshVI ATCGAT 2 cut(s) 1301, 3018
BsiHKAI GWGCWC 4 cut(s) 1089, 1425, 2848, 3577
BsiHKCI CYCGRG 1 cut(s) 1004
BsiSI CCGG 4 cut(s) 214, 711, 1121, 2036
BslFI GGGAC 5 cut(s) 50, 212, 452, 994, 3140
BslI CCNNNNNNNGG 8 cut(s) 1250, 1841, 2064, 2153, 2226, 3236, 3468, 3522
BsmAI GTCTC 4 cut(s) 252, 1001, 1879, 2524
BsmFI GGGAC 5 cut(s) 50, 212, 452, 994, 3140
BsmI GAATGC 3 cut(s) 970, 1693, 2673
BsnI GGCC 7 cut(s) 335, 605, 894, 945, 1120, 2072, 3207
BsoBI CYCGRG 1 cut(s) 1004
Bsp119I TTCGAA 3 cut(s) 346, 691, 2677
Bsp1286I GDGCHC 7 cut(s) 220, 1089, 1425, 1467, 1827, 2848, 3577
Bsp13I TCCGGA 1 cut(s) 2035
Bsp1407I TGTACA 1 cut(s) 2353
Bsp19I CCATGG 1 cut(s) 462
BspANI GGCC 7 cut(s) 335, 605, 894, 945, 1120, 2072, 3207
BspCNI CTCAG 6 cut(s) 1471, 1588, 2080, 2488, 2987, 3476
BspDI ATCGAT 2 cut(s) 1301, 3018
BspEI TCCGGA 1 cut(s) 2035
BspFNI CGCG 2 cut(s) 3052, 3243
BspHI TCATGA 2 cut(s) 165, 1741
BspLI GGNNCC 7 cut(s) 66, 685, 859, 1109, 1880, 2949, 3540
BspMAI CTGCAG 1 cut(s) 2125
BspMI ACCTGC 2 cut(s) 2117, 2294
BspPI GGATC 9 cut(s) 41, 852, 865, 1028, 1235, 1826, 2027, 2119, 2375
BspQI GCTCTTC 2 cut(s) 25, 1405
BspT104I TTCGAA 3 cut(s) 346, 691, 2677
BspT107I GGYRCC 1 cut(s) 1107
BsrDI GCAATG 4 cut(s) 282, 3354, 3440, 3516
BsrGI TGTACA 1 cut(s) 2353
BssECI CCNNGG 7 cut(s) 462, 552, 940, 1587, 2276, 2622, 2967
BssNI GRCGYC 1 cut(s) 1095
BssT1I CCWWGG 4 cut(s) 462, 1587, 2622, 2967
Bst2UI CCWGG 4 cut(s) 554, 941, 1117, 2277
Bst6I CTCTTC 4 cut(s) 25, 1395, 1405, 3384
BstACI GRCGYC 1 cut(s) 1095
BstAPI GCANNNNNTGC 3 cut(s) 2660, 3410, 3419
BstAUI TGTACA 1 cut(s) 2353
BstBAI YACGTR 2 cut(s) 632, 2721
BstBI TTCGAA 3 cut(s) 346, 691, 2677
BstC8I GCNNGC 9 cut(s) 642, 658, 723, 1425, 1793, 2070, 2868, 3008, 3368
BstDEI CTNAG 9 cut(s) 240, 1295, 1458, 1596, 2088, 2475, 2974, 3012, 3463
BstDSI CCRYGG 1 cut(s) 462
BstF5I GGATG 4 cut(s) 318, 545, 1129, 1597
BstFNI CGCG 2 cut(s) 3052, 3243
BstH2I RGCGCY 4 cut(s) 1587, 1936, 2540, 2796
BstHHI GCGC 6 cut(s) 1586, 1935, 2539, 2795, 3054, 3424
BstMAI GTCTC 4 cut(s) 252, 1001, 1879, 2524
BstNI CCWGG 4 cut(s) 554, 941, 1117, 2277
BstNSI RCATGY 1 cut(s) 1157
BstSCI CCNGG 5 cut(s) 213, 552, 939, 1115, 2275
BstSFI CTRYAG 3 cut(s) 296, 1653, 2121
BstSLI GKGCMC 1 cut(s) 3577
BstSNI TACGTA 1 cut(s) 2721
BstUI CGCG 2 cut(s) 3052, 3243
BstV2I GAAGAC 2 cut(s) 2143, 2412
BstX2I RGATCY 4 cut(s) 857, 1871, 1901, 2111
BstYI RGATCY 4 cut(s) 857, 1871, 1901, 2111
Bsu15I ATCGAT 2 cut(s) 1301, 3018
Bsu36I CCTNAGG 1 cut(s) 1458
BsuI GTATCC 1 cut(s) 2710
BsuRI GGCC 7 cut(s) 335, 605, 894, 945, 1120, 2072, 3207
BsuTUI ATCGAT 2 cut(s) 1301, 3018
BtgI CCRYGG 1 cut(s) 462
BtgZI GCGATG 3 cut(s) 1479, 1652, 3045
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 4 cut(s) 318, 545, 1129, 1597
BtsI GCAGTG 2 cut(s) 1902, 3190
BtsIMutI CAGTG 8 cut(s) 408, 1218, 1622, 1902, 3190, 3471, 3577, 3750
BveI ACCTGC 2 cut(s) 2117, 2294
Cac8I GCNNGC 9 cut(s) 642, 658, 723, 1425, 1793, 2070, 2868, 3008, 3368
CaiI CAGNNNCTG 2 cut(s) 989, 1250
CciI TCATGA 2 cut(s) 165, 1741
CfoI GCGC 6 cut(s) 1586, 1935, 2539, 2795, 3054, 3424
Cfr13I GGNCC 4 cut(s) 64, 943, 2741, 3538
ClaI ATCGAT 2 cut(s) 1301, 3018
CseI GACGC 3 cut(s) 178, 2465, 3590
CspCI CAANNNNNGTGG 6 cut(s) 778, 813, 1686, 1721, 2631, 2666
DdeI CTNAG 9 cut(s) 240, 1295, 1458, 1596, 2088, 2475, 2974, 3012, 3463
DraI TTTAAA 1 cut(s) 1545
DrdI GACNNNNNNGTC 1 cut(s) 955
DseDI GACNNNNNNGTC 1 cut(s) 955
EaeI YGGCCR 1 cut(s) 603
Eam1104I CTCTTC 4 cut(s) 25, 1395, 1405, 3384
EarI CTCTTC 4 cut(s) 25, 1395, 1405, 3384
EciI GGCGGA 2 cut(s) 1778, 3510
Ecl136II GAGCTC 3 cut(s) 1087, 1423, 2846
Eco105I TACGTA 1 cut(s) 2721
Eco130I CCWWGG 4 cut(s) 462, 1587, 2622, 2967
Eco147I AGGCCT 1 cut(s) 3207
Eco24I GRGCYC 6 cut(s) 220, 1089, 1425, 1467, 1827, 2848
Eco47I GGWCC 3 cut(s) 64, 2741, 3538
Eco47III AGCGCT 1 cut(s) 2538
Eco53kI GAGCTC 3 cut(s) 1087, 1423, 2846
Eco72I CACGTG 1 cut(s) 632
Eco81I CCTNAGG 1 cut(s) 1458
Eco88I CYCGRG 1 cut(s) 1004
EcoICRI GAGCTC 3 cut(s) 1087, 1423, 2846
EcoO109I RGGNCCY 2 cut(s) 64, 3538
EcoRI GAATTC 3 cut(s) 342, 1557, 2325
EcoRII CCWGG 4 cut(s) 552, 939, 1115, 2275
EcoT14I CCWWGG 4 cut(s) 462, 1587, 2622, 2967
EcoT22I ATGCAT 3 cut(s) 970, 2827, 3769
EcoT38I GRGCYC 6 cut(s) 220, 1089, 1425, 1467, 1827, 2848
ErhI CCWWGG 4 cut(s) 462, 1587, 2622, 2967
FalI AAGNNNNNCTT 4 cut(s) 528, 560, 2404, 2436
FaqI GGGAC 5 cut(s) 50, 212, 452, 994, 3140
FauI CCCGC 2 cut(s) 583, 1132
FauNDI CATATG 2 cut(s) 848, 1375
FbaI TGATCA 1 cut(s) 867
FokI GGATG 4 cut(s) 325, 552, 1136, 1604
FriOI GRGCYC 6 cut(s) 220, 1089, 1425, 1467, 1827, 2848
FspAI RTGCGCAY 1 cut(s) 3423
FspBI CTAG 5 cut(s) 1554, 1950, 2534, 3578, 3789
FspI TGCGCA 1 cut(s) 3423
GlaI GCGC 6 cut(s) 1585, 1934, 2538, 2794, 3053, 3423
GsuI CTGGAG 5 cut(s) 432, 1071, 1953, 2548, 3081
HaeII RGCGCY 4 cut(s) 1587, 1936, 2540, 2796
HaeIII GGCC 7 cut(s) 335, 605, 894, 945, 1120, 2072, 3207
HapII CCGG 4 cut(s) 214, 711, 1121, 2036
HgaI GACGC 3 cut(s) 178, 2465, 3590
HhaI GCGC 6 cut(s) 1586, 1935, 2539, 2795, 3054, 3424
Hin1I GRCGYC 1 cut(s) 1095
Hin6I GCGC 6 cut(s) 1584, 1933, 2537, 2793, 3052, 3422
HinP1I GCGC 6 cut(s) 1584, 1933, 2537, 2793, 3052, 3422
HincII GTYRAC 3 cut(s) 43, 627, 1209
HindII GTYRAC 3 cut(s) 43, 627, 1209
HindIII AAGCTT 1 cut(s) 349
HpaI GTTAAC 1 cut(s) 43
HpaII CCGG 4 cut(s) 214, 711, 1121, 2036
Hpy166II GTNNAC 8 cut(s) 43, 262, 627, 635, 1209, 1508, 2356, 3575
Hpy8I GTNNAC 8 cut(s) 43, 262, 627, 635, 1209, 1508, 2356, 3575
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 9 cut(s) 55, 1375, 1760, 2020, 2434, 2701, 2732, 3156, 3715
HpyCH4IV ACGT 6 cut(s) 105, 631, 809, 1095, 2720, 3595
HpyF3I CTNAG 9 cut(s) 240, 1295, 1458, 1596, 2088, 2475, 2974, 3012, 3463
HpySE526I ACGT 6 cut(s) 105, 631, 809, 1095, 2720, 3595
Hsp92I GRCGYC 1 cut(s) 1095
HspAI GCGC 6 cut(s) 1584, 1933, 2537, 2793, 3052, 3422
Kpn2I TCCGGA 1 cut(s) 2035
KpnI GGTACC 1 cut(s) 1111
Ksp22I TGATCA 1 cut(s) 867
KspAI GTTAAC 1 cut(s) 43
LguI GCTCTTC 2 cut(s) 25, 1405
MaeI CTAG 5 cut(s) 1554, 1950, 2534, 3578, 3789
MaeII ACGT 6 cut(s) 105, 631, 809, 1095, 2720, 3595
MaeIII GTNAC 3 cut(s) 316, 558, 2453
MfeI CAATTG 2 cut(s) 1527, 2664
MflI RGATCY 4 cut(s) 857, 1871, 1901, 2111
MhlI GDGCHC 7 cut(s) 220, 1089, 1425, 1467, 1827, 2848, 3577
MlsI TGGCCA 1 cut(s) 605
MluNI TGGCCA 1 cut(s) 605
MlyI GAGTC 5 cut(s) 1607, 2210, 2948, 2966, 3347
MmeI TCCRAC 6 cut(s) 290, 661, 778, 1581, 2821, 3151
Mox20I TGGCCA 1 cut(s) 605
Mph1103I ATGCAT 3 cut(s) 970, 2827, 3769
MroI TCCGGA 1 cut(s) 2035
MroXI GAANNNNTTC 1 cut(s) 3324
MscI TGGCCA 1 cut(s) 605
MslI CAYNNNNRTG 2 cut(s) 2180, 3464
Msp20I TGGCCA 1 cut(s) 605
MspA1I CMGCKG 2 cut(s) 986, 2918
MspI CCGG 4 cut(s) 214, 711, 1121, 2036
MspR9I CCNGG 5 cut(s) 215, 554, 941, 1117, 2277
MunI CAATTG 2 cut(s) 1527, 2664
Mva1269I GAATGC 3 cut(s) 970, 1693, 2673
MvaI CCWGG 4 cut(s) 554, 941, 1117, 2277
MvnI CGCG 2 cut(s) 3052, 3243
NciI CCSGG 1 cut(s) 215
NcoI CCATGG 1 cut(s) 462
NdeI CATATG 2 cut(s) 848, 1375
NlaIV GGNNCC 7 cut(s) 66, 685, 859, 1109, 1880, 2949, 3540
NmeAIII GCCGAG 1 cut(s) 3742
NmuCI GTSAC 1 cut(s) 2453
NsbI TGCGCA 1 cut(s) 3423
NsiI ATGCAT 3 cut(s) 970, 2827, 3769
NspI RCATGY 1 cut(s) 1157
NspV TTCGAA 3 cut(s) 346, 691, 2677
OliI CACNNNNGTG 1 cut(s) 3464
PaeR7I CTCGAG 1 cut(s) 1004
PagI TCATGA 2 cut(s) 165, 1741
PaqCI CACCTGC 1 cut(s) 2294
PceI AGGCCT 1 cut(s) 3207
PciSI GCTCTTC 2 cut(s) 25, 1405
PcsI WCGNNNNNNNCGW 1 cut(s) 637
PctI GAATGC 3 cut(s) 970, 1693, 2673
PdmI GAANNNNTTC 1 cut(s) 3324
PfeI GAWTC 5 cut(s) 597, 1143, 3020, 3219, 3587
PflFI GACNNNGTC 1 cut(s) 3080
PflMI CCANNNNNTGG 3 cut(s) 1250, 1841, 2226
PleI GAGTC 5 cut(s) 1606, 2210, 2948, 2966, 3346
PmaCI CACGTG 1 cut(s) 632
PmlI CACGTG 1 cut(s) 632
PpsI GAGTC 5 cut(s) 1606, 2210, 2948, 2966, 3346
Ppu21I YACGTR 2 cut(s) 632, 2721
PpuMI RGGWCCY 2 cut(s) 64, 3538
PshBI ATTAAT 1 cut(s) 2828
PsiI TTATAA 1 cut(s) 2543
Psp124BI GAGCTC 3 cut(s) 1089, 1425, 2848
Psp5II RGGWCCY 2 cut(s) 64, 3538
Psp6I CCWGG 4 cut(s) 552, 939, 1115, 2275
PspCI CACGTG 1 cut(s) 632
PspGI CCWGG 4 cut(s) 552, 939, 1115, 2275
PspN4I GGNNCC 7 cut(s) 66, 685, 859, 1109, 1880, 2949, 3540
PspPI GGNCC 4 cut(s) 64, 943, 2741, 3538
PspPPI RGGWCCY 2 cut(s) 64, 3538
PsrI GAACNNNNNNTAC 2 cut(s) 3585, 3617
PstI CTGCAG 1 cut(s) 2125
PstNI CAGNNNCTG 2 cut(s) 989, 1250
PsuI RGATCY 4 cut(s) 857, 1871, 1901, 2111
PsyI GACNNNGTC 1 cut(s) 3080
PvuII CAGCTG 1 cut(s) 986
RseI CAYNNNNRTG 2 cut(s) 2180, 3464
SacI GAGCTC 3 cut(s) 1089, 1425, 2848
SapI GCTCTTC 2 cut(s) 25, 1405
Sau96I GGNCC 4 cut(s) 64, 943, 2741, 3538
ScaI AGTACT 1 cut(s) 1260
SchI GAGTC 5 cut(s) 1607, 2210, 2948, 2966, 3347
ScrFI CCNGG 5 cut(s) 215, 554, 941, 1117, 2277
SduI GDGCHC 7 cut(s) 220, 1089, 1425, 1467, 1827, 2848, 3577
SfcI CTRYAG 3 cut(s) 296, 1653, 2121
Sfr274I CTCGAG 1 cut(s) 1004
SfuI TTCGAA 3 cut(s) 346, 691, 2677
SinI GGWCC 3 cut(s) 64, 2741, 3538
SlaI CTCGAG 1 cut(s) 1004
SmiMI CAYNNNNRTG 2 cut(s) 2180, 3464
SmlI CTYRAG 3 cut(s) 1004, 1395, 3447
SmoI CTYRAG 3 cut(s) 1004, 1395, 3447
SnaBI TACGTA 1 cut(s) 2721
SseBI AGGCCT 1 cut(s) 3207
SspI AATATT 1 cut(s) 2988
SspMI CTAG 5 cut(s) 1554, 1950, 2534, 3578, 3789
SstI GAGCTC 3 cut(s) 1089, 1425, 2848
StuI AGGCCT 1 cut(s) 3207
StyD4I CCNGG 5 cut(s) 213, 552, 939, 1115, 2275
StyI CCWWGG 4 cut(s) 462, 1587, 2622, 2967
TaiI ACGT 6 cut(s) 108, 634, 812, 1098, 2723, 3598
TatI WGTACW 7 cut(s) 261, 354, 767, 1258, 1984, 2353, 3601
TauI GCSGC 1 cut(s) 2197
TfiI GAWTC 5 cut(s) 597, 1143, 3020, 3219, 3587
TscAI CASTG 8 cut(s) 415, 1218, 1629, 1902, 3190, 3471, 3577, 3757
TseFI GTSAC 1 cut(s) 2453
Tsp45I GTSAC 1 cut(s) 2453
TspGWI ACGGA 1 cut(s) 3270
TspRI CASTG 8 cut(s) 415, 1218, 1629, 1902, 3190, 3471, 3577, 3757
Tth111I GACNNNGTC 1 cut(s) 3080
Van91I CCANNNNNTGG 3 cut(s) 1250, 1841, 2226
VneI GTGCAC 1 cut(s) 3573
VpaK11BI GGWCC 3 cut(s) 64, 2741, 3538
VspI ATTAAT 1 cut(s) 2828
XapI RAATTY 8 cut(s) 254, 342, 1557, 2284, 2325, 2389, 2594, 2994
XceI RCATGY 1 cut(s) 1157
XcmI CCANNNNNNNNNTGG 1 cut(s) 2065
XhoI CTCGAG 1 cut(s) 1004
XmnI GAANNNNTTC 1 cut(s) 3324
XspI CTAG 5 cut(s) 1554, 1950, 2534, 3578, 3789
ZraI GACGTC 1 cut(s) 1096
ZrmI AGTACT 1 cut(s) 1260
Zsp2I ATGCAT 3 cut(s) 970, 2827, 3769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.