FvH4_7g10050

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001944
Physical Location & Seq
Reverse (-)
9496896 .. 9502474
5579 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g10050.t1

Sequence Viewer

Length: 3807 bp
ATGAAAGGAGGAAAAGGAAAAGAAAAAGGAAAAGGAAAAGATGAAGATGGAGATGTAGAGAAGCAGAAGGTCCCATTGTACAAGCTGTTTCAGTTTGCAGACCAATATGACGTCGCGTTGATGGTCGTCGGGAGTGTCTCTGCCATCGGAAACGGGTTGTCGCAGCCGTTCATGACGCTAATCTTCGGCAATCTTATCAATACTTTCGGAGCTACCGACCGAGAACATATCGTCCCCACGATTTCTAAGGTGGCCTTAAAGTTCGTATACTTGGGCATTGGCACTGGAATTGCTGCATTTTTACAGGTCTCATGTTGGATGGTAACTGGCGAAAGACAAGCCACTCGAATTCGGAGCATGTACTTGAAAGCAATTTTAAGACAGGACATTGGATATTTTGACACCGAAACCAACACCGGAGAGATCATTGGCAGAATGTCAGGGGACACAATACTCATTCAAGATGCAATGGGAGAAAAGGTGGGGAAGTTCATTCAACTCCTTTCAACTTTTGTTGGTGGTTTTATAATTGCTTTCGTCAAGGGATGGCGTCTCACCCTGGTATTATTGGCATGTATTCCTGCTATTGTCTTTGCTGGTGGAATCATGGCCATAATTGTTTCTAAAATGTCAACTCGTGGCCAACAAGCTTATGCAGAAGCTGGCACTATAGTAGAACAGACAGTTGGAGCCATTCGAACGGTTGCATCGTTTACCGGAGAGAAGCAAGCGATAGAGAGATATAACAAGAAGCTAAAGATCGCCTACAATAGTACAGTTCAACAAGGACTAGCCACAGGAATTGGACTTGGTACATTCTTGCTTATTGTCTTTGGCACTTACGGACTTGCCATATGGTACGGGTCCAAAATGATCATCGAAAAAGGATACAACGGAGGCCAAGTTATCAACGTTATATTTGCAATCATGACTGGTGGAATGTGTCTGGGGCAGACATCACCATCTTTGAATGCATTTGCAGCAGGAACAGCTGCTGCATACAAAATGTTCGAGACCATCAACCGAACCCCGAAGATTGATCCTTATGATAATTCTGGAGTTGTATTGGATGATATTAAAGGAGAGGTGGAACTTAAAGATGTGTATTTTAGGTATCCTGCTAGGCCAGATGTGCAGATTTTTTCTGGATTTTCTTTGCATGTTCCGAGTGGCACAACGACTGCTTTGGTTGGGCAAAGTGGAAGTGGGAAATCTACAGTGATAGGCTTGGTAGAGAGGTTTTATGATCCAGAAGCTGGTGAAGTGCTTATAGATGGTGTCAACTTGAAGAAGTTGCATCTGAGATCGATAAGGGAGAAGATTGGACTTGTAAGTCAAGAGCCAAATCTTTTTACTACAACATTAAGGGAGAACATAGCATATGGTAAGGAAAATGCAACTGAAGAAGAGATTAGAAGAGCAACTGAACTCGCAAATGCAGCAAAGTTTATTGACAAGTTGCCTCAGGGGATCGACACAATGGTGGGTGAGCATGGAACATCATTATCGGGTGGACAAAAGCAAAGAATTGCGATTGCAAGAGCAATTTTGAAGAACCCTAGAATTCTACTTCTGGATGAAGCAACAAGCGCCTTGGATGCTGAGTCAGAGCGAATTGTTCAAGATGCACTGGTGAGATTGATGTCAAATAGGACGACTATAGTTGTTGCACATCGCTTAACAACGATTAGGAATGCTGATGCAATAGCTGTGGTGCATACAGGAAAAATTGTTGAAATAGGAACTCATGATGAATTAACCAGAAACCCAGAAGGAGCTTATAGCCAATTGATTCGCCTACAAGAAGGAGTTAAAGAAAAAGATGCACAGCCCTCTGATCCGGATAATGTTTTGGATATGGATAAGCCAATGAGTAGGTCTGAAAGCCTGAGACTTTCAACAACGAGATCTTTAAGTAAGGGTTCATCTGGTAGTCGGCGCTCATTCACTATTACTAGCTTTGGTGTTCCTGCTCCAATTAATTGTGAAGTGCAAGTAGAAGAAGAAGATGAGGAGAACCTTGCAAAAACTAAGGTTGATCCGGAGCAGCGCAAAAAAGTTTCCATCAAAAGGTTGGCCTACTTGAATAAACCAGAGCTTCCAGTTTTGCTGCTTGGATCTATTGCTGCAGCAGGTCATGGAGTGATTTTTCCTGTCTTCGGCTTACTACTCTCCAAAGCCATAAAGATGTTTTATGAACCGCATAATAAGCTACAAAAAGACTCTATTGTTTGGGCTGGAGTGTATGTTGGTATAGGTTTGTTCGGGTTTGCTATAATCCCAGGACAAAATTTCTTCTTTGGAATCGCAGGTGGAAAATTAATAGAGCGAATTCGTGCCTTGACATTCCAGAAGGTTGTGCACCAACAAATTAGCTGGTTTGATGATCCTGCAAATTCAAGTGGTGCTATTGGTGCAAGGTTGTCTTCTGATGCTTCAACCGTGAAGGCCCTTGTTGGTGATGCATTAGCCTTGATTGTCCAGAACATAGCAACAATCATAGCAGGGTTAACTATAGGTTTCACAGCTAACTGGAGACTAGCGCTTATAATTATAGCTGTGTCGCCGTTGTTGATCGGGCAAGGAATACTTCAAACAAAATTTCTTAAAGGGTTTAGTGCTGATGCCAAGCTTATGTATGAAGAAGCAAGTCAAGTGGCAAATGACGCAATTGGTAGTATCCGAACTGTTGCATCATTTGGTTCTGAAAAGAAGGTAATGGATGCATATGAGAAGAAGTGTGAAGGTCCAATGAAACAAGGAGTAAGATTGGGAGTTGTTAGTGGTACAGGCTTTGGCGCTTCATTTGTAGTAATGTTCTTCACAAACGCATTAATTTTCTATGTTGGAGCTCATTTAGTGAAAAGTGGGCAAGCCACATTTGAACAAGTATTCAAGGTTTTCTTTGCTTTAACAATTTCAGCGGTTGGGGTTTCACAATCTACTGGCATGGCCCCTGACTCCAACAAAGCCAAGGACTCAGCAGCTTCAATATTTCAAATTCTTGATAGCAAGCCTAAGATCGATTCAAGCAGCGATGCAGGCATAACACTACCGAGTATTACTGGAGAAATTGATGTTGAACATATTAGTTTCAGATACCCCACTAGGCCAGATGTGCAGATATTTAGGGACATTTGTTTGAAATTCCCTCCTGGAAAGACTGTTGCTTTGGCTGGAGAGAGTGGCAGTGGTAAATCAACAATCATAGGCCTTATAGAGCGATTCTATGATCCTGATACAGGTCGCATACTATTAGATGGAGTTGAAATCCACAAGTTCAAGATAAACTGGTTAAGGCAACAAATTGGTTTGGTTGGTCAAGAACCTATTCTCTTTAATGAATCAATACGTACCAACATTGCCTACGGCAAGCAAGGAGATGCAACCGAAGAAGAGATCATAGCTGCAACAAAAGCATCAAATGCGCACAATTTCATATCTTCATTACCTCAAGGATATGACACGTCGGTAGGGGAACGAGGTGTTCAATTGTCAGGTGGACAAAAACAAAGAATAGCTATTGCAAGGGCTATACTAAAGGACCCCAAAATCCTTTTACTTGATGAAGCTACTAGTGCGCTAGACGCAGAGTCAGAACGCATTGTGCAAGATGCTTTAGACACAGTTATGGTGAATAGAACAACTATTGTTGTGGCTCACCGCCTGACCACGATCAAAGGGGCAGATGTAATCGCTGTGGTGAAGAATGGTGTGATTGCTGAGAAGGGAAGCCATGATATTCTAATGAAGATTACTGATGGGGCTTATGCATCTTTGGTGGCACTTCACGCTAGCGCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1269

Amino Acids

136.82

Weight (kDa)

8.61

Isoelectric Point (pI)

33.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 43 - 312 9.6e-64 ABC transporter transmembrane region
ABC_tran PF00005 383 - 530 7.7e-33 ABC transporter
ABC_membrane PF00664 704 - 975 1.5e-59 ABC transporter transmembrane region
ABC_tran PF00005 1044 - 1193 1.4e-32 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 527, 2549
AarI CACCTGC 1 cut(s) 2300
AasI GACNNNNNNGTC 1 cut(s) 1332
AatII GACGTC 1 cut(s) 114
Acc16I TGCGCA 1 cut(s) 3429
Acc36I ACCTGC 2 cut(s) 2123, 2300
AccB7I CCANNNNNTGG 1 cut(s) 1256
AccI GTMKAC 1 cut(s) 267
AccII CGCG 1 cut(s) 116
AccIII TCCGGA 2 cut(s) 1840, 2041
AciI CCGC 3 cut(s) 2201, 2924, 3664
AclI AACGTT 1 cut(s) 912
AclWI GGATC 8 cut(s) 1034, 1241, 1478, 1832, 2033, 2125, 2381, 3227
AcoI YGGCCR 2 cut(s) 609, 640
AcsI RAATTY 8 cut(s) 348, 1563, 2290, 2331, 2395, 2600, 3000, 3146
AcuI CTGAAG 1 cut(s) 1422
AcyI GRCGYC 2 cut(s) 111, 550
AfaI GTAC 7 cut(s) 80, 362, 775, 814, 860, 2788, 3355
AfeI AGCGCT 1 cut(s) 2544
AfiI CCNNNNNNNGG 4 cut(s) 1256, 2070, 2159, 3242
AflIII ACRYGT 1 cut(s) 3465
AhdI GACNNNNNGTC 1 cut(s) 3592
AhlI ACTAGT 1 cut(s) 3575
AjiI CACGTC 1 cut(s) 3468
AjnI CCWGG 3 cut(s) 558, 2281, 3154
AjuI GAANNNNNNNTTGG 2 cut(s) 669, 701
AleI CACNNNNGTG 1 cut(s) 1481
AloI GAACNNNNNNTCC 2 cut(s) 216, 248
Alw21I GWGCWC 2 cut(s) 2364, 2854
Alw26I GTCTC 6 cut(s) 142, 313, 557, 1007, 1885, 2530
Alw44I GTGCAC 1 cut(s) 2360
AlwI GGATC 8 cut(s) 1034, 1241, 1478, 1832, 2033, 2125, 2381, 3227
AlwNI CAGNNNCTG 3 cut(s) 662, 995, 1256
Aor13HI TCCGGA 2 cut(s) 1840, 2041
Aor51HI AGCGCT 1 cut(s) 2544
ApaLI GTGCAC 1 cut(s) 2360
ApoI RAATTY 8 cut(s) 348, 1563, 2290, 2331, 2395, 2600, 3000, 3146
ArsI GACNNNNNNTTYG 2 cut(s) 3154, 3186
AseI ATTAAT 3 cut(s) 1980, 2321, 2834
Asp700I GAANNNNTTC 1 cut(s) 3330
AspLEI GCGC 8 cut(s) 1592, 1941, 2052, 2545, 2801, 3430, 3583, 3800
AspS9I GGNCC 6 cut(s) 70, 864, 2449, 2747, 2953, 3544
AsuHPI GGTGA 9 cut(s) 547, 951, 1271, 1499, 1645, 2471, 3646, 3653, 3715
AsuII TTCGAA 1 cut(s) 697
AsuNHI GCTAGC 1 cut(s) 3794
AvaII GGWCC 4 cut(s) 70, 864, 2747, 3544
AxyI CCTNAGG 1 cut(s) 1464
BaeGI GKGCMC 1 cut(s) 2364
BalI TGGCCA 2 cut(s) 611, 642
BanII GRGCYC 1 cut(s) 2854
BauI CACGAG 1 cut(s) 636
BbsI GAAGAC 2 cut(s) 2149, 2418
Bbv12I GWGCWC 2 cut(s) 2364, 2854
BceAI ACGGC 3 cut(s) 151, 2551, 3385
BciT130I CCWGG 3 cut(s) 560, 2283, 3156
BciVI GTATCC 3 cut(s) 881, 1125, 2690
BclI TGATCA 1 cut(s) 873
BcoDI GTCTC 6 cut(s) 142, 313, 557, 1007, 1885, 2530
BcuI ACTAGT 1 cut(s) 3575
BfaI CTAG 9 cut(s) 791, 1122, 1560, 1956, 2540, 3108, 3576, 3584, 3795
BfmI CTRYAG 5 cut(s) 669, 1215, 1659, 2127, 2514
BfoI RGCGCY 4 cut(s) 1593, 1942, 2546, 2802
BfuAI ACCTGC 2 cut(s) 2123, 2300
BfuI GTATCC 3 cut(s) 881, 1125, 2690
BglII AGATCT 1 cut(s) 1907
Bme1390I CCNGG 3 cut(s) 560, 2283, 3156
Bme18I GGWCC 4 cut(s) 70, 864, 2747, 3544
BmeRI GACNNNNNGTC 1 cut(s) 3592
BmgBI CACGTC 1 cut(s) 3468
BmgT120I GGNCC 6 cut(s) 70, 864, 2449, 2747, 2953, 3544
BmiI GGNNCC 5 cut(s) 72, 691, 865, 2955, 3546
BmrFI CCNGG 3 cut(s) 560, 2283, 3156
BmtI GCTAGC 1 cut(s) 3798
BpiI GAAGAC 2 cut(s) 2149, 2418
BpmI CTGGAG 5 cut(s) 1077, 2259, 2554, 3087, 3198
Bpu14I TTCGAA 1 cut(s) 697
BpuEI CTTGAG 1 cut(s) 3438
Bsa29I ATCGAT 2 cut(s) 1307, 3024
BsaAI YACGTR 1 cut(s) 3353
BsaHI GRCGYC 2 cut(s) 111, 550
BsaI GGTCTC 2 cut(s) 313, 1007
BsaJI CCNNGG 4 cut(s) 558, 1593, 2281, 2973
BsaWI WCCGGW 4 cut(s) 416, 716, 1840, 2041
BsaXI ACNNNNNCTCC 6 cut(s) 465, 495, 888, 918, 2232, 2262
Bsc4I CCNNNNNNNGG 4 cut(s) 1256, 2070, 2159, 3242
Bse1I ACTGG 9 cut(s) 289, 331, 937, 1635, 2102, 2537, 2950, 3070, 3296
Bse21I CCTNAGG 1 cut(s) 1464
Bse3DI GCAATG 2 cut(s) 474, 3360
BseAI TCCGGA 2 cut(s) 1840, 2041
BseBI CCWGG 3 cut(s) 560, 2283, 3156
BseCI ATCGAT 2 cut(s) 1307, 3024
BseDI CCNNGG 4 cut(s) 558, 1593, 2281, 2973
BseGI GGATG 6 cut(s) 324, 551, 1075, 1582, 1603, 2728
BseLI CCNNNNNNNGG 4 cut(s) 1256, 2070, 2159, 3242
BseMI GCAATG 2 cut(s) 474, 3360
BseMII CTCAG 6 cut(s) 1292, 1478, 1593, 1880, 2994, 3714
BseNI ACTGG 9 cut(s) 289, 331, 937, 1635, 2102, 2537, 2950, 3070, 3296
BseRI GAGGAG 1 cut(s) 2027
BseSI GKGCMC 1 cut(s) 2364
BsgI GTGCAG 2 cut(s) 1154, 3140
Bsh1236I CGCG 1 cut(s) 116
Bsh1285I CGRYCG 1 cut(s) 220
BshVI ATCGAT 2 cut(s) 1307, 3024
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 2 cut(s) 2364, 2854
BsiSI CCGG 4 cut(s) 417, 717, 1841, 2042
BslFI GGGAC 4 cut(s) 56, 218, 458, 3146
BslI CCNNNNNNNGG 4 cut(s) 1256, 2070, 2159, 3242
BsmAI GTCTC 6 cut(s) 142, 313, 557, 1007, 1885, 2530
BsmBI CGTCTC 1 cut(s) 557
BsmFI GGGAC 4 cut(s) 56, 218, 458, 3146
BsmI GAATGC 2 cut(s) 976, 1699
Bso31I GGTCTC 2 cut(s) 313, 1007
Bsp119I TTCGAA 1 cut(s) 697
Bsp1286I GDGCHC 2 cut(s) 2364, 2854
Bsp13I TCCGGA 2 cut(s) 1840, 2041
Bsp1407I TGTACA 1 cut(s) 78
BspACI CCGC 3 cut(s) 2201, 2924, 3664
BspCNI CTCAG 6 cut(s) 1293, 1477, 1594, 1881, 2993, 3715
BspDI ATCGAT 2 cut(s) 1307, 3024
BspEI TCCGGA 2 cut(s) 1840, 2041
BspFNI CGCG 1 cut(s) 116
BspHI TCATGA 4 cut(s) 171, 927, 1747, 3803
BspLI GGNNCC 5 cut(s) 72, 691, 865, 2955, 3546
BspMAI CTGCAG 1 cut(s) 2131
BspMI ACCTGC 2 cut(s) 2123, 2300
BspOI GCTAGC 1 cut(s) 3798
BspPI GGATC 8 cut(s) 1034, 1241, 1478, 1832, 2033, 2125, 2381, 3227
BspQI GCTCTTC 1 cut(s) 1411
BspT104I TTCGAA 1 cut(s) 697
BspTNI GGTCTC 2 cut(s) 313, 1007
BsrDI GCAATG 2 cut(s) 474, 3360
BsrGI TGTACA 1 cut(s) 78
BsrI ACTGG 9 cut(s) 289, 331, 937, 1635, 2102, 2537, 2950, 3070, 3296
BssECI CCNNGG 4 cut(s) 558, 1593, 2281, 2973
BssNAI GTATAC 1 cut(s) 268
BssNI GRCGYC 2 cut(s) 111, 550
BssSI CACGAG 1 cut(s) 636
BssT1I CCWWGG 2 cut(s) 1593, 2973
Bst1107I GTATAC 1 cut(s) 268
Bst2BI CACGAG 1 cut(s) 636
Bst2UI CCWGG 3 cut(s) 560, 2283, 3156
Bst4CI ACNGT 8 cut(s) 685, 703, 778, 1219, 2443, 2689, 3166, 3628
Bst6I CTCTTC 3 cut(s) 1401, 1411, 3390
BstACI GRCGYC 2 cut(s) 111, 550
BstAPI GCANNNNNTGC 1 cut(s) 3425
BstAUI TGTACA 1 cut(s) 78
BstBAI YACGTR 1 cut(s) 3353
BstBI TTCGAA 1 cut(s) 697
BstC8I GCNNGC 7 cut(s) 664, 729, 2874, 3014, 3043, 3374, 3796
BstDEI CTNAG 9 cut(s) 246, 1301, 1464, 1602, 1889, 2031, 2980, 3018, 3723
BstENI CCTNNNNNAGG 1 cut(s) 3240
BstF5I GGATG 6 cut(s) 324, 551, 1075, 1582, 1603, 2728
BstFNI CGCG 1 cut(s) 116
BstH2I RGCGCY 4 cut(s) 1593, 1942, 2546, 2802
BstHHI GCGC 8 cut(s) 1592, 1941, 2052, 2545, 2801, 3430, 3583, 3800
BstMAI GTCTC 6 cut(s) 142, 313, 557, 1007, 1885, 2530
BstMCI CGRYCG 1 cut(s) 220
BstNI CCWGG 3 cut(s) 560, 2283, 3156
BstNSI RCATGY 3 cut(s) 361, 576, 1163
BstSCI CCNGG 3 cut(s) 558, 2281, 3154
BstSFI CTRYAG 5 cut(s) 669, 1215, 1659, 2127, 2514
BstSLI GKGCMC 1 cut(s) 2364
BstSNI TACGTA 1 cut(s) 3353
BstUI CGCG 1 cut(s) 116
BstV2I GAAGAC 2 cut(s) 2149, 2418
BstX2I RGATCY 2 cut(s) 1907, 2117
BstYI RGATCY 2 cut(s) 1907, 2117
BstZ17I GTATAC 1 cut(s) 268
Bsu15I ATCGAT 2 cut(s) 1307, 3024
Bsu36I CCTNAGG 1 cut(s) 1464
BsuI GTATCC 3 cut(s) 881, 1125, 2690
BsuTUI ATCGAT 2 cut(s) 1307, 3024
BtgZI GCGATG 2 cut(s) 1658, 3051
BtrI CACGTC 1 cut(s) 3468
BtsCI GGATG 6 cut(s) 324, 551, 1075, 1582, 1603, 2728
BtsI GCAGTG 1 cut(s) 3196
BtsIMutI CAGTG 4 cut(s) 282, 1224, 1628, 3196
BveI ACCTGC 2 cut(s) 2123, 2300
Cac8I GCNNGC 7 cut(s) 664, 729, 2874, 3014, 3043, 3374, 3796
CaiI CAGNNNCTG 3 cut(s) 662, 995, 1256
CciI TCATGA 4 cut(s) 171, 927, 1747, 3803
CfoI GCGC 8 cut(s) 1592, 1941, 2052, 2545, 2801, 3430, 3583, 3800
Cfr13I GGNCC 6 cut(s) 70, 864, 2449, 2747, 2953, 3544
ClaI ATCGAT 2 cut(s) 1307, 3024
CseI GACGC 4 cut(s) 184, 539, 2675, 3596
Csp6I GTAC 7 cut(s) 79, 361, 774, 813, 859, 2787, 3354
CspCI CAANNNNNGTGG 6 cut(s) 784, 819, 1692, 1727, 2637, 2672
CviQI GTAC 7 cut(s) 79, 361, 774, 813, 859, 2787, 3354
DdeI CTNAG 9 cut(s) 246, 1301, 1464, 1602, 1889, 2031, 2980, 3018, 3723
DrdI GACNNNNNNGTC 1 cut(s) 1332
DriI GACNNNNNGTC 1 cut(s) 3592
DseDI GACNNNNNNGTC 1 cut(s) 1332
EaeI YGGCCR 2 cut(s) 609, 640
Eam1104I CTCTTC 3 cut(s) 1401, 1411, 3390
Eam1105I GACNNNNNGTC 1 cut(s) 3592
EarI CTCTTC 3 cut(s) 1401, 1411, 3390
Ecl136II GAGCTC 1 cut(s) 2852
Eco105I TACGTA 1 cut(s) 3353
Eco130I CCWWGG 2 cut(s) 1593, 2973
Eco147I AGGCCT 1 cut(s) 3213
Eco24I GRGCYC 1 cut(s) 2854
Eco31I GGTCTC 2 cut(s) 313, 1007
Eco47I GGWCC 4 cut(s) 70, 864, 2747, 3544
Eco47III AGCGCT 1 cut(s) 2544
Eco53kI GAGCTC 1 cut(s) 2852
Eco57I CTGAAG 1 cut(s) 1422
Eco81I CCTNAGG 1 cut(s) 1464
EcoICRI GAGCTC 1 cut(s) 2852
EcoNI CCTNNNNNAGG 1 cut(s) 3240
EcoO109I RGGNCCY 3 cut(s) 70, 2449, 3544
EcoRI GAATTC 3 cut(s) 348, 1563, 2331
EcoRII CCWGG 3 cut(s) 558, 2281, 3154
EcoT14I CCWWGG 2 cut(s) 1593, 2973
EcoT22I ATGCAT 4 cut(s) 976, 2467, 2728, 3775
EcoT38I GRGCYC 1 cut(s) 2854
ErhI CCWWGG 2 cut(s) 1593, 2973
Esp3I CGTCTC 1 cut(s) 557
FalI AAGNNNNNCTT 8 cut(s) 239, 271, 2410, 2442, 2574, 2606, 2888, 2920
FaqI GGGAC 4 cut(s) 56, 218, 458, 3146
FauNDI CATATG 3 cut(s) 854, 1381, 2728
FbaI TGATCA 1 cut(s) 873
FblI GTMKAC 1 cut(s) 267
FokI GGATG 6 cut(s) 331, 558, 1082, 1589, 1610, 2735
FriOI GRGCYC 1 cut(s) 2854
FspAI RTGCGCAY 1 cut(s) 3429
FspBI CTAG 9 cut(s) 791, 1122, 1560, 1956, 2540, 3108, 3576, 3584, 3795
FspI TGCGCA 1 cut(s) 3429
GlaI GCGC 8 cut(s) 1591, 1940, 2051, 2544, 2800, 3429, 3582, 3799
GsuI CTGGAG 5 cut(s) 1077, 2259, 2554, 3087, 3198
HaeII RGCGCY 4 cut(s) 1593, 1942, 2546, 2802
HapII CCGG 4 cut(s) 417, 717, 1841, 2042
HgaI GACGC 4 cut(s) 184, 539, 2675, 3596
HhaI GCGC 8 cut(s) 1592, 1941, 2052, 2545, 2801, 3430, 3583, 3800
Hin1I GRCGYC 2 cut(s) 111, 550
Hin6I GCGC 8 cut(s) 1590, 1939, 2050, 2543, 2799, 3428, 3581, 3798
HinP1I GCGC 8 cut(s) 1590, 1939, 2050, 2543, 2799, 3428, 3581, 3798
HincII GTYRAC 3 cut(s) 633, 1282, 2511
HindII GTYRAC 3 cut(s) 633, 1282, 2511
HindIII AAGCTT 2 cut(s) 648, 2630
HpaI GTTAAC 1 cut(s) 2511
HpaII CCGG 4 cut(s) 417, 717, 1841, 2042
HphI GGTGA 9 cut(s) 547, 951, 1271, 1499, 1645, 2471, 3646, 3653, 3715
Hpy166II GTNNAC 8 cut(s) 268, 633, 714, 1282, 1514, 2362, 2511, 3503
Hpy8I GTNNAC 8 cut(s) 268, 633, 714, 1282, 1514, 2362, 2511, 3503
Hpy99I CGWCG 3 cut(s) 116, 131, 3472
HpyAV CCTTC 8 cut(s) 61, 1766, 1799, 2347, 2440, 2707, 2738, 3721
HpyCH4III ACNGT 8 cut(s) 685, 703, 778, 1219, 2443, 2689, 3166, 3628
HpyCH4IV ACGT 4 cut(s) 111, 912, 3352, 3467
HpyF3I CTNAG 9 cut(s) 246, 1301, 1464, 1602, 1889, 2031, 2980, 3018, 3723
HpySE526I ACGT 4 cut(s) 111, 912, 3352, 3467
Hsp92I GRCGYC 2 cut(s) 111, 550
HspAI GCGC 8 cut(s) 1590, 1939, 2050, 2543, 2799, 3428, 3581, 3798
Kpn2I TCCGGA 2 cut(s) 1840, 2041
Ksp22I TGATCA 1 cut(s) 873
KspAI GTTAAC 1 cut(s) 2511
LguI GCTCTTC 1 cut(s) 1411
LmnI GCTCC 7 cut(s) 209, 354, 689, 1775, 1978, 2044, 2849
MaeI CTAG 9 cut(s) 791, 1122, 1560, 1956, 2540, 3108, 3576, 3584, 3795
MaeII ACGT 4 cut(s) 111, 912, 3352, 3467
MaeIII GTNAC 1 cut(s) 322
MfeI CAATTG 3 cut(s) 1787, 2670, 3491
MflI RGATCY 2 cut(s) 1907, 2117
MhlI GDGCHC 2 cut(s) 2364, 2854
MlsI TGGCCA 2 cut(s) 611, 642
MluNI TGGCCA 2 cut(s) 611, 642
MlyI GAGTC 5 cut(s) 1613, 2216, 2954, 2972, 3602
MmeI TCCRAC 4 cut(s) 296, 667, 2827, 2988
MnlI CCTC 9 cut(s) 890, 1078, 1230, 1473, 1843, 2005, 3162, 3462, 3476
Mox20I TGGCCA 2 cut(s) 611, 642
Mph1103I ATGCAT 4 cut(s) 976, 2467, 2728, 3775
MroI TCCGGA 2 cut(s) 1840, 2041
MroXI GAANNNNTTC 1 cut(s) 3330
MscI TGGCCA 2 cut(s) 611, 642
MslI CAYNNNNRTG 3 cut(s) 1481, 2186, 3629
Msp20I TGGCCA 2 cut(s) 611, 642
MspA1I CMGCKG 2 cut(s) 992, 2924
MspI CCGG 4 cut(s) 417, 717, 1841, 2042
MspR9I CCNGG 3 cut(s) 560, 2283, 3156
MunI CAATTG 3 cut(s) 1787, 2670, 3491
Mva1269I GAATGC 2 cut(s) 976, 1699
MvaI CCWGG 3 cut(s) 560, 2283, 3156
MvnI CGCG 1 cut(s) 116
NdeI CATATG 3 cut(s) 854, 1381, 2728
NheI GCTAGC 1 cut(s) 3794
NlaIV GGNNCC 5 cut(s) 72, 691, 865, 2955, 3546
NsbI TGCGCA 1 cut(s) 3429
NsiI ATGCAT 4 cut(s) 976, 2467, 2728, 3775
NspI RCATGY 3 cut(s) 361, 576, 1163
NspV TTCGAA 1 cut(s) 697
OliI CACNNNNGTG 1 cut(s) 1481
PagI TCATGA 4 cut(s) 171, 927, 1747, 3803
PaqCI CACCTGC 1 cut(s) 2300
PceI AGGCCT 1 cut(s) 3213
PciSI GCTCTTC 1 cut(s) 1411
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 707
PctI GAATGC 2 cut(s) 976, 1699
PdmI GAANNNNTTC 1 cut(s) 3330
PfeI GAWTC 6 cut(s) 603, 1792, 2304, 3026, 3225, 3344
PflMI CCANNNNNTGG 1 cut(s) 1256
PfoI TCCNGGA 1 cut(s) 3154
PleI GAGTC 5 cut(s) 1612, 2216, 2954, 2972, 3601
PpsI GAGTC 5 cut(s) 1612, 2216, 2954, 2972, 3601
Ppu21I YACGTR 1 cut(s) 3353
PpuMI RGGWCCY 2 cut(s) 70, 3544
PshBI ATTAAT 3 cut(s) 1980, 2321, 2834
PsiI TTATAA 2 cut(s) 527, 2549
Psp124BI GAGCTC 1 cut(s) 2854
Psp1406I AACGTT 1 cut(s) 912
Psp5II RGGWCCY 2 cut(s) 70, 3544
Psp6I CCWGG 3 cut(s) 558, 2281, 3154
PspGI CCWGG 3 cut(s) 558, 2281, 3154
PspN4I GGNNCC 5 cut(s) 72, 691, 865, 2955, 3546
PspPI GGNCC 6 cut(s) 70, 864, 2449, 2747, 2953, 3544
PspPPI RGGWCCY 2 cut(s) 70, 3544
PstI CTGCAG 1 cut(s) 2131
PstNI CAGNNNCTG 3 cut(s) 662, 995, 1256
PsuI RGATCY 2 cut(s) 1907, 2117
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 7 cut(s) 80, 362, 775, 814, 860, 2788, 3355
RsaNI GTAC 7 cut(s) 79, 361, 774, 813, 859, 2787, 3354
RseI CAYNNNNRTG 3 cut(s) 1481, 2186, 3629
SacI GAGCTC 1 cut(s) 2854
SapI GCTCTTC 1 cut(s) 1411
Sau96I GGNCC 6 cut(s) 70, 864, 2449, 2747, 2953, 3544
SchI GAGTC 5 cut(s) 1613, 2216, 2954, 2972, 3602
ScrFI CCNGG 3 cut(s) 560, 2283, 3156
SduI GDGCHC 2 cut(s) 2364, 2854
SfcI CTRYAG 5 cut(s) 669, 1215, 1659, 2127, 2514
SfuI TTCGAA 1 cut(s) 697
SinI GGWCC 4 cut(s) 70, 864, 2747, 3544
SmiMI CAYNNNNRTG 3 cut(s) 1481, 2186, 3629
SmlI CTYRAG 1 cut(s) 3453
SmoI CTYRAG 1 cut(s) 3453
SnaBI TACGTA 1 cut(s) 3353
SpeI ACTAGT 1 cut(s) 3575
SseBI AGGCCT 1 cut(s) 3213
SsiI CCGC 3 cut(s) 2201, 2924, 3664
SspI AATATT 1 cut(s) 2994
SspMI CTAG 9 cut(s) 791, 1122, 1560, 1956, 2540, 3108, 3576, 3584, 3795
SstI GAGCTC 1 cut(s) 2854
StuI AGGCCT 1 cut(s) 3213
StyD4I CCNGG 3 cut(s) 558, 2281, 3154
StyI CCWWGG 2 cut(s) 1593, 2973
TaaI ACNGT 8 cut(s) 685, 703, 778, 1219, 2443, 2689, 3166, 3628
TaiI ACGT 4 cut(s) 114, 915, 3355, 3470
TaqI TCGA 7 cut(s) 346, 697, 879, 1011, 1307, 1473, 3024
TaqII GACCGA 1 cut(s) 234
TatI WGTACW 3 cut(s) 78, 360, 773
TfiI GAWTC 6 cut(s) 603, 1792, 2304, 3026, 3225, 3344
TscAI CASTG 4 cut(s) 289, 1224, 1635, 3196
TspGWI ACGGA 2 cut(s) 858, 909
TspRI CASTG 4 cut(s) 289, 1224, 1635, 3196
Van91I CCANNNNNTGG 1 cut(s) 1256
VneI GTGCAC 1 cut(s) 2360
VpaK11BI GGWCC 4 cut(s) 70, 864, 2747, 3544
VspI ATTAAT 3 cut(s) 1980, 2321, 2834
XagI CCTNNNNNAGG 1 cut(s) 3240
XapI RAATTY 8 cut(s) 348, 1563, 2290, 2331, 2395, 2600, 3000, 3146
XceI RCATGY 3 cut(s) 361, 576, 1163
XcmI CCANNNNNNNNNTGG 1 cut(s) 2071
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 3330
XspI CTAG 9 cut(s) 791, 1122, 1560, 1956, 2540, 3108, 3576, 3584, 3795
ZraI GACGTC 1 cut(s) 112
Zsp2I ATGCAT 4 cut(s) 976, 2467, 2728, 3775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.