MD10G1268300.v1.1

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001944
Physical Location & Seq
Forward (+)
36101009 .. 36110081
9073 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1268300.v1.1.491

Sequence Viewer

Length: 3837 bp
ATGGAAGGAGGAAGCGGAGGAGGAGATGGAAAAGCAGAAGTTAATAATAATAGCGGGTCACAAGCGAAAGCAGATCAAGAAAAGCAGAAAGTGGCCTTCTATAAACTCTTTGCGTTTGCAGACCGTTTGGACATTGCGTTGATGATCGTTGGAACGATATCTGCGGTGGGGAATGGTCTGTCGCAGCCACTCATGACTCTTGTGTTTGGTAATCTTATCAACACCTTTGGTTCCACCGATCCTGGTCACATCGTTCCCTTGATCTCTAAGGTGTCTCTAAAATTTGTGTACTTGGCCATTGGCACTGGAGCTGCAGCACTTCTACAGGTGGCATGTTGGATGGTGACAGGAGAAAGGCAGGCTGCTAGAATTAGAGGCAAGTACATGAAGGCAATCCTAAGGCAGGACATTGGCTTCTTTGACACTGAAACTAGCACTGGAGAGATCATTGGCAGGATGTCTGGGGACACCATTCTTATTCAAGAAGCCATGGGGGAAAAGGTGGGGAAATTTATACAACTTTGTTCTACCTTTCTTGGTGGTTTTATGATTGCTTTTGTCAAAGGGTGGCGCCTAGCCATTGTATTGCTGGCTACCATTCCTGCCATTGTCATTGCTGGTGCAACCATGGCTACTATCATGGCGAGAATGTCATCCCGCGGACAGACTGCTTATGCAGAAGCTGGCAATATAGTAGAACAAACAGTTGGTTCCATTAGAACAGTTGCATCTTTCACTGGGGAGAAGCAAGCGATTGAAAAGTACAACCAGAAGCTAAAAGTTGCTTACAATACTATGGTTCAACAAGGGCTAGTCACAGGGCTTGGACTCGGAATATTTATGCTCGTCATCTTCTGCACGTACGCGCTTGCGGTATGGTATGGATCCAAAATGATCATCAGACATGGATACAATGGAGGCCAAGTTATCAATGTTATATTTGCACTCATGACTGGCGGAATGTCACTCGGTCAGGCATCACCATCGTTGAATGCATTCGCATCAGGGAAAGCTGCTGCATATAAAATGTTTGAGACCATCAGCCGAAATCCAACTATAGATCCCTACGATGCAGGTGGAATTGTATTGCAAGATGTTAAAGGTGATGTCGAACTCAAAGACGTGTATTTCAGGTACCCTGCCAGACCAGACGTGAAGATCTTTGTTGGGTTCTCATTATATGTTCCAAGTGGCACAACCACTGCTTTGGTTGGGCAGAGTGGAAGTGGGAAGTCGACAGTGATCGGGCTAGTAGAAAGGTTTTATGATCCAGAAGCCGGTGAAGTACTTATAGATGGCGTGGACTTGAAGAAGTTGCAGCTTAAAGCGATAAGAGAGAAGATTGGGCTGGTTAGTCAGGAACCGAATCTCTTTACAACAACCATAAGGGAGAACATAAAATATGGGAAGGATGATGCAACTGATGAGGAGATTAGAAGAGCAACTGAGCTCGCAAATGCAGCAAAATTCATTGATAAGCTACCCCAGGGGATCGACACAATGGTAGGTGAGCACGGGACATCTCTATCTGGTGGGCAAAAGCAGAGAATTGCAATTGCAAGAGCCATTCTAAAGAACCCGAGAATTCTTCTTCTTGATGAAGCAACAAGCGCGCTGGATGCTGAGTCCGAAAAGATTGTTCAAGATGCACTGGTGAACTTGATGTCAAATCGCACAACCATAGTTGTTGCGCATCGCTTGACAACTATTAGGAATGCTGATTGCATAGCAGTGGTGCACAGAGGCAAAATTGTTGAAAAAGGGACTCATAATGAGTTGACCAAAGATCCGGAAGGGGCTTACAGCCAATTAATCCGCCTGCAAGAAGGATCAAAAGTTGACAACGAAGCACAAACCTCTGATCCGAATAGAATGGACACCAGCCTAGATACAGATAGGACTTTGCTCAGCTCTGGGAGCCGGAGATTATCAATGAGGAGGTCTATAAGCAGAGGTTCATCAGGTAACCGGCACTCATTTACTATTGGCTTTGGCATTCCCGGTCCAGTTGATATCCAAGAGAGTGAAGTAGGACACGAGGATGATCATGACAGACCTAAAGTTGATCCTGAAAAGCGCAAAACAGTTTCTATCCGAAGGCTGGCTGCACTTAACAAACCTGAGGCTCCAGTTTTGCTTCTAGGAGCCATTGCTGCAGCAGGGCATGGAGTAATTTTCCCTATCTTTGCGTTACTACTCTCGAAAGCCATTAGAATGTTCTACGAACCGCCCAATGAGCTAAGGCATGATTCCAGAAAATGGGCGTTAGTTTATGTAGGTTTAGGTTGCGCTGCTCTGTTGGTGATTCCTGTGCAGAATTTCTTCTTTGGAGTTGCAGGTGGGAAATTAGTGGAGCGAATTCGTTCCTTGTCATTCCAGAAGGTTGTGCACCAGCAAGTCAGTTGGTTTGATGACCCTGCAAATTCAAGCGGTGCGATTGGTGCAAGGTTATCTACGGATGCTTCAACCATTAAGAGTCTTGTTGGTGACGCCTTAGCGTTGATTGTGCAGAATATAGCAACAATCATCGCAGGGCTGATTATAGGTTTCACAGCTAACTGGAAACTGACGCTGGTAGTTTTAGCCGTTTCGCCATTGATGCTTCTGCAAGGAACACTTCAGGCAAAATTTCTTAAAGGGTTTAGTGCAGATGCCAAGCTTATGTATGAGGAAGCCAGTCAAGTGGCAAACGATGCGATTGGTAGCATCCGAACGGTTGCATCTTTTTGTTCTGAGAAGAAGGTGATAGAGGCATACGAGAAGAAGTGTGAGGGTCCGTTGAAGCAAGGAGTACGGTTAGGAGTAGTAAGTGGTTCGGGTTTTGGCTTTTCGTTCTTCTTAATGTTCTGCACAAATGCATTGGTATTCTATGTTGGAGCTATTCTTGTGAAGCATGGTCAAGCTACATTTGAACAAGTATTCAAGGTTTTCTTCGCTGTAACAATCTCTGCAATGGGGGTCTCACAATCTACTAGCATGGCCCCTGACTCCAACAAAGCCAAGGACTCATCGGCTTCGATATTTCAAATTCTCGACAGCAAACCTAAGATTGACTCGAGCAGTAATGAAGGCGTAACATTATCACATTTAATTGGAGACATCGAGCTTGAGCATGTCAGCTTTAAGTATCCTATGCGCCCGGATGTGCAGATATTCAGAGACATTTGTTTGAAGATGCCCTCCGGAAAGACTGTTGCTTTGGTTGGAGAGAGTGGCAGCGGGAAATCAACGGTAATAGGCCTCATAGAGAGATTCTACGATCCTGATTCAGGTCGCGTACTACTAGACGGAGTAGAAATCCAAAAGTTCAAGCTAAACTGGCTGAGGCAGCAGATAGGGTTGGTTGGTCAAGAACCAGTTCTCTTCAACGAATCCATTCGTGCCAACATTGCATATGGCAAGCCAGGAGATGTTACCGAGGAAGAAATCATAGCAGCGACCACAGCAGCTAACGTGCACAATGTCATATCTTCATTGCCTCAAGGCTACGACACCTCAGTAGGTGAACGAGGGGTTCAACTGTCAGGAGGTCAGAAGCAAAGAATTGCCATTGCGAGGGCTATACTCAAGGACCCGAAAATCCTCCTACTTGATGAAGCTACAAGTGCACTGGATGCAGAGTCCGAACGCAAAGTACAAGACGCATTGGACAGGGTTATGGTGAACAGAACAACAGTTGTAGTTGCGCACCGCCTTAGCACGATAAAAGGAGCAGACATAATCGCGGTGGTGAAGAATGGCGTGATTGCGGAGAAGGGAAGCCATGATTTCCTAATGAAGATCACTGACGGGGCTTATGCTTCTTTGGTGGCACTTAATTCCAGCTCAAATACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1279

Amino Acids

137.63

Weight (kDa)

8.76

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 48 - 319 8.4e-62 ABC transporter transmembrane region
ABC_tran PF00005 391 - 537 2.4e-33 ABC transporter
ABC_membrane PF00664 712 - 985 1.8e-61 ABC transporter transmembrane region
ABC_tran PF00005 1053 - 1202 5.7e-33 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1064, 2327
Acc16I TGCGCA 2 cut(s) 1692, 3687
Acc36I ACCTGC 2 cut(s) 1064, 2327
Acc65I GGTACC 1 cut(s) 1134
AccB1I GGYRCC 2 cut(s) 570, 1134
AccB7I CCANNNNNTGG 1 cut(s) 2259
AccI GTMKAC 1 cut(s) 1235
AccII CGCG 5 cut(s) 660, 866, 1613, 3276, 3725
AccIII TCCGGA 2 cut(s) 1789, 3182
AcoI YGGCCR 1 cut(s) 294
AcsI RAATTY 9 cut(s) 281, 509, 1466, 1584, 2317, 2358, 2422, 2627, 3027
AcuI CTGAAG 1 cut(s) 2603
AcyI GRCGYC 2 cut(s) 571, 2490
AfaI GTAC 9 cut(s) 290, 383, 764, 863, 1136, 1287, 2793, 3279, 3636
AfiI CCNNNNNNNGG 6 cut(s) 403, 1277, 2101, 2259, 3269, 3555
AflIII ACRYGT 1 cut(s) 1122
AjiI CACGTC 2 cut(s) 1123, 1153
AjnI CCWGG 3 cut(s) 241, 1485, 3403
AjuI GAANNNNNNNTTGG 2 cut(s) 690, 722
Alw21I GWGCWC 6 cut(s) 1452, 1515, 1740, 2391, 3459, 3610
Alw26I GTCTC 5 cut(s) 279, 1028, 2965, 3090, 3153
Alw44I GTGCAC 4 cut(s) 1736, 2387, 3455, 3606
AlwNI CAGNNNCTG 1 cut(s) 683
Ama87I CYCGRG 2 cut(s) 1579, 3055
Aor13HI TCCGGA 2 cut(s) 1789, 3182
AoxI GGCC 5 cut(s) 93, 294, 919, 2979, 3238
ApaLI GTGCAC 4 cut(s) 1736, 2387, 3455, 3606
ApoI RAATTY 9 cut(s) 281, 509, 1466, 1584, 2317, 2358, 2422, 2627, 3027
ArsI GACNNNNNNTTYG 2 cut(s) 3181, 3213
AseI ATTAAT 1 cut(s) 1811
Asp700I GAANNNNTTC 5 cut(s) 995, 2321, 2362, 3357, 3375
Asp718I GGTACC 1 cut(s) 1134
AspLEI GCGC 9 cut(s) 573, 868, 1613, 1615, 1693, 2079, 2291, 3138, 3688
AspS9I GGNCC 4 cut(s) 2003, 2774, 2980, 3571
AsuC2I CCSGG 2 cut(s) 2001, 3140
AvaI CYCGRG 2 cut(s) 1579, 3055
AvaII GGWCC 3 cut(s) 2003, 2774, 3571
AxyI CCTNAGG 2 cut(s) 398, 2121
BaeGI GKGCMC 4 cut(s) 1740, 2391, 3459, 3610
BalI TGGCCA 1 cut(s) 296
BamHI GGATCC 1 cut(s) 884
BanI GGYRCC 2 cut(s) 570, 1134
BanII GRGCYC 1 cut(s) 1452
BauI CACGAG 1 cut(s) 2036
Bbv12I GWGCWC 6 cut(s) 1452, 1515, 1740, 2391, 3459, 3610
BbvCI CCTCAGC 1 cut(s) 3323
BccI CCATC 5 cut(s) 20, 334, 991, 1046, 1289
BceAI ACGGC 1 cut(s) 2570
BcgI CGANNNNNNTGC 2 cut(s) 966, 1000
BciT130I CCWGG 3 cut(s) 243, 1487, 3405
BciVI GTATCC 2 cut(s) 902, 3138
BclI TGATCA 2 cut(s) 894, 2044
BcnI CCSGG 2 cut(s) 2001, 3140
BcoDI GTCTC 5 cut(s) 279, 1028, 2965, 3090, 3153
BfaI CTAG 9 cut(s) 366, 432, 575, 812, 1250, 1886, 2141, 2973, 3284
BfmI CTRYAG 4 cut(s) 312, 323, 1056, 2154
BfoI RGCGCY 1 cut(s) 574
BfuAI ACCTGC 2 cut(s) 1064, 2327
BfuI GTATCC 2 cut(s) 902, 3138
BglII AGATCT 1 cut(s) 1158
BlpI GCTNAGC 1 cut(s) 1907
BmcAI AGTACT 1 cut(s) 1287
Bme1390I CCNGG 5 cut(s) 243, 1487, 2001, 3140, 3405
Bme18I GGWCC 3 cut(s) 2003, 2774, 3571
BmeT110I CYCGRG 2 cut(s) 1579, 3055
BmgBI CACGTC 2 cut(s) 1123, 1153
BmgT120I GGNCC 4 cut(s) 2003, 2774, 2980, 3571
BmrFI CCNGG 5 cut(s) 243, 1487, 2001, 3140, 3405
BmrI ACTGGG 1 cut(s) 747
BmuI ACTGGG 1 cut(s) 747
BpmI CTGGAG 3 cut(s) 327, 459, 2112
Bpu10I CCTNAGC 4 cut(s) 2240, 2494, 3323, 3695
Bpu1102I GCTNAGC 1 cut(s) 1907
BpuEI CTTGAG 3 cut(s) 3128, 3465, 3551
BpuMI CCSGG 2 cut(s) 2001, 3140
BsaAI YACGTR 1 cut(s) 861
BsaHI GRCGYC 2 cut(s) 571, 2490
BsaI GGTCTC 2 cut(s) 1028, 2965
BsaJI CCNNGG 7 cut(s) 489, 627, 658, 1485, 1486, 3000, 3417
BsaWI WCCGGW 2 cut(s) 1789, 3182
BsaXI ACNNNNNCTCC 2 cut(s) 909, 939
Bsc4I CCNNNNNNNGG 6 cut(s) 403, 1277, 2101, 2259, 3269, 3555
Bse118I RCCGGY 2 cut(s) 1277, 1968
Bse21I CCTNAGG 2 cut(s) 398, 2121
Bse3DI GCAATG 7 cut(s) 132, 612, 2148, 2958, 3387, 3473, 3549
BseAI TCCGGA 2 cut(s) 1789, 3182
BseBI CCWGG 3 cut(s) 243, 1487, 3405
BseDI CCNNGG 7 cut(s) 489, 627, 658, 1485, 1486, 3000, 3417
BseLI CCNNNNNNNGG 6 cut(s) 403, 1277, 2101, 2259, 3269, 3555
BseMI GCAATG 7 cut(s) 132, 612, 2148, 2958, 3387, 3473, 3549
BseMII CTCAG 7 cut(s) 1437, 1614, 1921, 2112, 2724, 3314, 3510
BsePI GCGCGC 1 cut(s) 1611
BseRI GAGGAG 4 cut(s) 33, 36, 1442, 1951
BseSI GKGCMC 4 cut(s) 1740, 2391, 3459, 3610
BsgI GTGCAG 7 cut(s) 841, 2091, 2333, 2528, 2667, 2833, 3167
Bsh1236I CGCG 5 cut(s) 660, 866, 1613, 3276, 3725
BshFI GGCC 5 cut(s) 95, 296, 921, 2981, 3240
BshNI GGYRCC 2 cut(s) 570, 1134
BsiHKAI GWGCWC 6 cut(s) 1452, 1515, 1740, 2391, 3459, 3610
BsiHKCI CYCGRG 2 cut(s) 1579, 3055
BsiSI CCGG 7 cut(s) 1278, 1790, 1921, 1969, 2001, 3140, 3183
BsiWI CGTACG 1 cut(s) 861
BslFI GGGAC 3 cut(s) 479, 1531, 1777
BslI CCNNNNNNNGG 6 cut(s) 403, 1277, 2101, 2259, 3269, 3555
BsmAI GTCTC 5 cut(s) 279, 1028, 2965, 3090, 3153
BsmFI GGGAC 3 cut(s) 479, 1531, 1777
BsmI GAATGC 4 cut(s) 995, 997, 1720, 1995
BsnI GGCC 5 cut(s) 95, 296, 921, 2981, 3240
Bso31I GGTCTC 2 cut(s) 1028, 2965
BsoBI CYCGRG 2 cut(s) 1579, 3055
Bsp1286I GDGCHC 6 cut(s) 1452, 1515, 1740, 2391, 3459, 3610
Bsp13I TCCGGA 2 cut(s) 1789, 3182
Bsp1720I GCTNAGC 1 cut(s) 1907
Bsp19I CCATGG 2 cut(s) 489, 627
BspANI GGCC 5 cut(s) 95, 296, 921, 2981, 3240
BspCNI CTCAG 7 cut(s) 1438, 1615, 1920, 2113, 2725, 3315, 3509
BspEI TCCGGA 2 cut(s) 1789, 3182
BspFNI CGCG 5 cut(s) 660, 866, 1613, 3276, 3725
BspHI TCATGA 3 cut(s) 192, 948, 2047
BspMAI CTGCAG 2 cut(s) 316, 2158
BspMI ACCTGC 2 cut(s) 1064, 2327
BspQI GCTCTTC 1 cut(s) 1432
BspT107I GGYRCC 2 cut(s) 570, 1134
BspTNI GGTCTC 2 cut(s) 1028, 2965
BsrDI GCAATG 7 cut(s) 132, 612, 2148, 2958, 3387, 3473, 3549
BsrFI RCCGGY 2 cut(s) 1277, 1968
BssAI RCCGGY 2 cut(s) 1277, 1968
BssECI CCNNGG 7 cut(s) 489, 627, 658, 1485, 1486, 3000, 3417
BssHII GCGCGC 1 cut(s) 1611
BssNI GRCGYC 2 cut(s) 571, 2490
BssSI CACGAG 1 cut(s) 2036
BssT1I CCWWGG 3 cut(s) 489, 627, 3000
Bst2BI CACGAG 1 cut(s) 2036
Bst2UI CCWGG 3 cut(s) 243, 1487, 3405
Bst6I CTCTTC 2 cut(s) 1432, 3368
BstACI GRCGYC 2 cut(s) 571, 2490
BstAPI GCANNNNNTGC 2 cut(s) 2693, 3614
BstBAI YACGTR 1 cut(s) 861
BstDSI CCRYGG 3 cut(s) 489, 627, 658
BstEII GGTNACC 1 cut(s) 1964
BstENI CCTNNNNNAGG 2 cut(s) 401, 3267
BstFNI CGCG 5 cut(s) 660, 866, 1613, 3276, 3725
BstH2I RGCGCY 1 cut(s) 574
BstHHI GCGC 9 cut(s) 573, 868, 1613, 1615, 1693, 2079, 2291, 3138, 3688
BstMAI GTCTC 5 cut(s) 279, 1028, 2965, 3090, 3153
BstNI CCWGG 3 cut(s) 243, 1487, 3405
BstNSI RCATGY 2 cut(s) 336, 3116
BstPI GGTNACC 1 cut(s) 1964
BstSCI CCNGG 5 cut(s) 241, 1485, 1999, 3138, 3403
BstSFI CTRYAG 4 cut(s) 312, 323, 1056, 2154
BstSLI GKGCMC 4 cut(s) 1740, 2391, 3459, 3610
BstUI CGCG 5 cut(s) 660, 866, 1613, 3276, 3725
BstX2I RGATCY 4 cut(s) 884, 1060, 1158, 1786
BstXI CCANNNNNNTGG 2 cut(s) 1207, 2683
BstYI RGATCY 4 cut(s) 884, 1060, 1158, 1786
Bsu36I CCTNAGG 2 cut(s) 398, 2121
BsuI GTATCC 2 cut(s) 902, 3138
BsuRI GGCC 5 cut(s) 95, 296, 921, 2981, 3240
BtgI CCRYGG 3 cut(s) 489, 627, 658
BtgZI GCGATG 2 cut(s) 1679, 2512
BtrI CACGTC 2 cut(s) 1123, 1153
BtsI GCAGTG 2 cut(s) 1200, 1737
BveI ACCTGC 2 cut(s) 1064, 2327
CaiI CAGNNNCTG 1 cut(s) 683
CciI TCATGA 3 cut(s) 192, 948, 2047
CfoI GCGC 9 cut(s) 573, 868, 1613, 1615, 1693, 2079, 2291, 3138, 3688
Cfr10I RCCGGY 2 cut(s) 1277, 1968
Cfr13I GGNCC 4 cut(s) 2003, 2774, 2980, 3571
Cfr42I CCGCGG 1 cut(s) 661
CseI GACGC 3 cut(s) 2498, 2578, 3650
Csp6I GTAC 9 cut(s) 289, 382, 763, 862, 1135, 1286, 2792, 3278, 3635
CviQI GTAC 9 cut(s) 289, 382, 763, 862, 1135, 1286, 2792, 3278, 3635
DinI GGCGCC 1 cut(s) 572
EaeI YGGCCR 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 1432, 3368
EarI CTCTTC 2 cut(s) 1432, 3368
EciI GGCGGA 2 cut(s) 972, 1805
Ecl136II GAGCTC 1 cut(s) 1450
Eco130I CCWWGG 3 cut(s) 489, 627, 3000
Eco147I AGGCCT 1 cut(s) 3240
Eco24I GRGCYC 1 cut(s) 1452
Eco31I GGTCTC 2 cut(s) 1028, 2965
Eco32I GATATC 2 cut(s) 159, 2014
Eco47I GGWCC 3 cut(s) 2003, 2774, 3571
Eco53kI GAGCTC 1 cut(s) 1450
Eco57I CTGAAG 1 cut(s) 2603
Eco81I CCTNAGG 2 cut(s) 398, 2121
Eco88I CYCGRG 2 cut(s) 1579, 3055
Eco91I GGTNACC 1 cut(s) 1964
EcoICRI GAGCTC 1 cut(s) 1450
EcoNI CCTNNNNNAGG 2 cut(s) 401, 3267
EcoO109I RGGNCCY 1 cut(s) 3571
EcoO65I GGTNACC 1 cut(s) 1964
EcoRI GAATTC 2 cut(s) 1584, 2358
EcoRII CCWGG 3 cut(s) 241, 1485, 3403
EcoRV GATATC 2 cut(s) 159, 2014
EcoT14I CCWWGG 3 cut(s) 489, 627, 3000
EcoT22I ATGCAT 2 cut(s) 997, 2860
EcoT38I GRGCYC 1 cut(s) 1452
EgeI GGCGCC 1 cut(s) 572
EheI GGCGCC 1 cut(s) 572
ErhI CCWWGG 3 cut(s) 489, 627, 3000
FalI AAGNNNNNCTT 4 cut(s) 2601, 2633, 2915, 2947
FaqI GGGAC 3 cut(s) 479, 1531, 1777
FauI CCCGC 3 cut(s) 47, 665, 3212
FauNDI CATATG 1 cut(s) 3394
FbaI TGATCA 2 cut(s) 894, 2044
FblI GTMKAC 1 cut(s) 1235
FriOI GRGCYC 1 cut(s) 1452
FspBI CTAG 9 cut(s) 366, 432, 575, 812, 1250, 1886, 2141, 2973, 3284
FspI TGCGCA 2 cut(s) 1692, 3687
GlaI GCGC 9 cut(s) 572, 867, 1612, 1614, 1692, 2078, 2290, 3137, 3687
GsuI CTGGAG 3 cut(s) 327, 459, 2112
HaeII RGCGCY 1 cut(s) 574
HaeIII GGCC 5 cut(s) 95, 296, 921, 2981, 3240
HapII CCGG 7 cut(s) 1278, 1790, 1921, 1969, 2001, 3140, 3183
HgaI GACGC 3 cut(s) 2498, 2578, 3650
HhaI GCGC 9 cut(s) 573, 868, 1613, 1615, 1693, 2079, 2291, 3138, 3688
Hin1I GRCGYC 2 cut(s) 571, 2490
Hin6I GCGC 9 cut(s) 571, 866, 1611, 1613, 1691, 2077, 2289, 3136, 3686
HinP1I GCGC 9 cut(s) 571, 866, 1611, 1613, 1691, 2077, 2289, 3136, 3686
HincII GTYRAC 3 cut(s) 1236, 1779, 1840
HindII GTYRAC 3 cut(s) 1236, 1779, 1840
HindIII AAGCTT 1 cut(s) 2657
HpaII CCGG 7 cut(s) 1278, 1790, 1921, 1969, 2001, 3140, 3183
HpyCH4IV ACGT 4 cut(s) 860, 1122, 1152, 3453
HpySE526I ACGT 4 cut(s) 860, 1122, 1152, 3453
Hsp92I GRCGYC 2 cut(s) 571, 2490
HspAI GCGC 9 cut(s) 571, 866, 1611, 1613, 1691, 2077, 2289, 3136, 3686
KasI GGCGCC 1 cut(s) 570
Kpn2I TCCGGA 2 cut(s) 1789, 3182
KpnI GGTACC 1 cut(s) 1138
Ksp22I TGATCA 2 cut(s) 894, 2044
KspI CCGCGG 1 cut(s) 661
LguI GCTCTTC 1 cut(s) 1432
LmnI GCTCC 7 cut(s) 308, 1917, 2131, 2144, 2353, 2876, 3710
MaeI CTAG 9 cut(s) 366, 432, 575, 812, 1250, 1886, 2141, 2973, 3284
MaeII ACGT 4 cut(s) 860, 1122, 1152, 3453
MfeI CAATTG 1 cut(s) 1554
MflI RGATCY 4 cut(s) 884, 1060, 1158, 1786
MhlI GDGCHC 6 cut(s) 1452, 1515, 1740, 2391, 3459, 3610
MlsI TGGCCA 1 cut(s) 296
MluNI TGGCCA 1 cut(s) 296
Mly113I GGCGCC 1 cut(s) 571
MlyI GAGTC 9 cut(s) 190, 822, 1634, 1759, 2485, 2981, 2999, 3047, 3629
MmeI TCCRAC 6 cut(s) 130, 317, 1076, 2854, 3015, 3184
Mox20I TGGCCA 1 cut(s) 296
Mph1103I ATGCAT 2 cut(s) 997, 2860
MroI TCCGGA 2 cut(s) 1789, 3182
MroXI GAANNNNTTC 5 cut(s) 995, 2321, 2362, 3357, 3375
MscI TGGCCA 1 cut(s) 296
MslI CAYNNNNRTG 3 cut(s) 1730, 2040, 2213
Msp20I TGGCCA 1 cut(s) 296
MspA1I CMGCKG 2 cut(s) 660, 3219
MspI CCGG 7 cut(s) 1278, 1790, 1921, 1969, 2001, 3140, 3183
MspR9I CCNGG 5 cut(s) 243, 1487, 2001, 3140, 3405
MunI CAATTG 1 cut(s) 1554
Mva1269I GAATGC 4 cut(s) 995, 997, 1720, 1995
MvaI CCWGG 3 cut(s) 243, 1487, 3405
MvnI CGCG 5 cut(s) 660, 866, 1613, 3276, 3725
NarI GGCGCC 1 cut(s) 571
NciI CCSGG 2 cut(s) 2001, 3140
NcoI CCATGG 2 cut(s) 489, 627
NdeI CATATG 1 cut(s) 3394
NmuCI GTSAC 6 cut(s) 57, 245, 343, 814, 963, 2486
NsbI TGCGCA 2 cut(s) 1692, 3687
NsiI ATGCAT 2 cut(s) 997, 2860
NspI RCATGY 2 cut(s) 336, 3116
PaeR7I CTCGAG 1 cut(s) 3055
PagI TCATGA 3 cut(s) 192, 948, 2047
PaqCI CACCTGC 2 cut(s) 1064, 2327
PasI CCCWGGG 1 cut(s) 1486
PauI GCGCGC 1 cut(s) 1611
PceI AGGCCT 1 cut(s) 3240
PciSI GCTCTTC 1 cut(s) 1432
PctI GAATGC 4 cut(s) 995, 997, 1720, 1995
PdmI GAANNNNTTC 5 cut(s) 995, 2321, 2362, 3357, 3375
PfeI GAWTC 6 cut(s) 1366, 2249, 2305, 3252, 3266, 3371
Pfl23II CGTACG 1 cut(s) 861
PflMI CCANNNNNTGG 1 cut(s) 2259
PleI GAGTC 9 cut(s) 190, 822, 1633, 1759, 2484, 2981, 2999, 3047, 3628
PluTI GGCGCC 1 cut(s) 574
PpsI GAGTC 9 cut(s) 190, 822, 1633, 1759, 2484, 2981, 2999, 3047, 3628
Ppu21I YACGTR 1 cut(s) 861
PpuMI RGGWCCY 1 cut(s) 3571
PshBI ATTAAT 1 cut(s) 1811
Psp124BI GAGCTC 1 cut(s) 1452
Psp5II RGGWCCY 1 cut(s) 3571
Psp6I CCWGG 3 cut(s) 241, 1485, 3403
PspEI GGTNACC 1 cut(s) 1964
PspGI CCWGG 3 cut(s) 241, 1485, 3403
PspLI CGTACG 1 cut(s) 861
PspPI GGNCC 4 cut(s) 2003, 2774, 2980, 3571
PspPPI RGGWCCY 1 cut(s) 3571
PspXI VCTCGAGB 1 cut(s) 3055
PsrI GAACNNNNNNTAC 2 cut(s) 3618, 3650
PstI CTGCAG 2 cut(s) 316, 2158
PstNI CAGNNNCTG 1 cut(s) 683
PsuI RGATCY 4 cut(s) 884, 1060, 1158, 1786
PteI GCGCGC 1 cut(s) 1611
RsaI GTAC 9 cut(s) 290, 383, 764, 863, 1136, 1287, 2793, 3279, 3636
RsaNI GTAC 9 cut(s) 289, 382, 763, 862, 1135, 1286, 2792, 3278, 3635
RseI CAYNNNNRTG 3 cut(s) 1730, 2040, 2213
SacI GAGCTC 1 cut(s) 1452
SacII CCGCGG 1 cut(s) 661
SalI GTCGAC 1 cut(s) 1234
SapI GCTCTTC 1 cut(s) 1432
Sau96I GGNCC 4 cut(s) 2003, 2774, 2980, 3571
ScaI AGTACT 1 cut(s) 1287
SchI GAGTC 9 cut(s) 190, 822, 1634, 1759, 2485, 2981, 2999, 3047, 3629
ScrFI CCNGG 5 cut(s) 243, 1487, 2001, 3140, 3405
SduI GDGCHC 6 cut(s) 1452, 1515, 1740, 2391, 3459, 3610
SfcI CTRYAG 4 cut(s) 312, 323, 1056, 2154
SfoI GGCGCC 1 cut(s) 572
Sfr274I CTCGAG 1 cut(s) 3055
Sfr303I CCGCGG 1 cut(s) 661
SgrBI CCGCGG 1 cut(s) 661
SinI GGWCC 3 cut(s) 2003, 2774, 3571
SlaI CTCGAG 1 cut(s) 3055
SmiMI CAYNNNNRTG 3 cut(s) 1730, 2040, 2213
SmlI CTYRAG 4 cut(s) 3055, 3107, 3480, 3566
SmoI CTYRAG 4 cut(s) 3055, 3107, 3480, 3566
SseBI AGGCCT 1 cut(s) 3240
SspDI GGCGCC 1 cut(s) 570
SspI AATATT 1 cut(s) 837
SspMI CTAG 9 cut(s) 366, 432, 575, 812, 1250, 1886, 2141, 2973, 3284
SstI GAGCTC 1 cut(s) 1452
StuI AGGCCT 1 cut(s) 3240
StyD4I CCNGG 5 cut(s) 241, 1485, 1999, 3138, 3403
StyI CCWWGG 3 cut(s) 489, 627, 3000
TaiI ACGT 4 cut(s) 863, 1125, 1155, 3456
TaqI TCGA 8 cut(s) 1110, 1235, 1494, 2201, 3017, 3033, 3056, 3102
TaqII GACCGA 1 cut(s) 959
TatI WGTACW 5 cut(s) 288, 381, 762, 1285, 3634
TfiI GAWTC 6 cut(s) 1366, 2249, 2305, 3252, 3266, 3371
TseFI GTSAC 6 cut(s) 57, 245, 343, 814, 963, 2486
Tsp45I GTSAC 6 cut(s) 57, 245, 343, 814, 963, 2486
TspDTI ATGAA 8 cut(s) 401, 1459, 1614, 1947, 3081, 3462, 3609, 3791
TspGWI ACGGA 3 cut(s) 2471, 2766, 3303
Van91I CCANNNNNTGG 1 cut(s) 2259
VneI GTGCAC 4 cut(s) 1736, 2387, 3455, 3606
VpaK11BI GGWCC 3 cut(s) 2003, 2774, 3571
VspI ATTAAT 1 cut(s) 1811
XagI CCTNNNNNAGG 2 cut(s) 401, 3267
XapI RAATTY 9 cut(s) 281, 509, 1466, 1584, 2317, 2358, 2422, 2627, 3027
XceI RCATGY 2 cut(s) 336, 3116
XcmI CCANNNNNNNNNTGG 1 cut(s) 586
XhoI CTCGAG 1 cut(s) 3055
XmiI GTMKAC 1 cut(s) 1235
XmnI GAANNNNTTC 5 cut(s) 995, 2321, 2362, 3357, 3375
XspI CTAG 9 cut(s) 366, 432, 575, 812, 1250, 1886, 2141, 2973, 3284
ZrmI AGTACT 1 cut(s) 1287
Zsp2I ATGCAT 2 cut(s) 997, 2860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.