AT5G65687

lipid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001699
Physical Location & Seq
Reverse (-)
26269808 .. 26273000
3193 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65687.1

Sequence Viewer

Length: 1479 bp
ATGACGAGAGTTGGCCAGAGAGATTCTCCGGCTAAAGAAGAAGCTCCGCCGGCGACTAAAAAACGATTTTTGACTCCGGGAAGATTCGTGACAATCTTGTGTATCATTAACTTGATAAATTACGTTGATCGAGGAGTGATCGCGAGTAATGGTGTTAATGGAAGCTCAAAAGTATGTGATGCCAAAGGGGTTTGCTCTGCAGGAACTGGAATTCAGGGAGAGTTTAACTTAACTAACTTTGAAGATGGTCTTTTGTCATCTGCGTTTATGGTTGGACTTCTTGTGGCTTCTCCAATATTCGCTGGATTGTCAAAAAGATTCAACCCTTTTAAACTAATCGGTGTTGGATTAACTGTTTGGACTATTGCTGTTATCGGTTGCGGGTTTTCCTACAACTTCTGGATGATTGCTGTTTTCAGAATGTTTGTTGGGGTTGGTGAGGCTTCCTTTATTAGTCTTGCAGCACCTTATATCGATGATAGTGCTCCTGTTGCAAGGAAAAATTTCTGGCTTGGACTGTTCTACATGTGTATACCAGCAGGAGTTGCTCTAGGCTATGTGTTTGGTGGATACATTGGAAACCATTTAGGATGGCGTTGGGCGTTTTACATAGAGGCAATCGCAATGGCTGTCTTCGTCATTTTGTCCTTCTGTATCAAACCACCGCAGCAGCTAAAAGGTTTTGCTGATAAGGATTCTAAGAAACCCTCTACATCTATTGAAACGGTTGCTCCTACAGATGCAGAAGCTTCACAAATTAAAACCAAAACCCCCAAATCCAAGAACCTGGTTGTCCTATTTGGAAAAGACTTAAAGGCTCTTTTCAGCGAGAAAGTATTTATTGTAAATGTTCTTGGTTACATCACCTACAACTTTGTGATTGGAGCTTACTCATATTGGGGACCAAAAGCCGGTTTTGGTATCTACAAAATGAAAAACGCGGACATGATTTTTGGAGGACTTACAATCATATGCGGAATAATTGGAACATTAGGTGGAAGCTATGTGCTTGATCGCATAAACGCTACTCTTTCAAACACCTTCAAGTTACTAGCTGCATCAACTTTACTCGGCGCAGCGTTTTGTTTCACTGCTTTCTTGATGAAGAATATGTATGCCTTCATCGCTTTATTCGCAGTGGGAGAAATTCTAATCTTTGCACCACAGGCTCCGGTTAACTTTGTGTGTCTCCATTGCGTGAGACCAAACTTGAGACCATTGTCAATGGCTTCTTCAACTGTTTTGATCCATATCTTAGGCGATGTTCCTTCTTCTCCACTTTATGGGAAAATGCAGGATCATTTAAAAAATTGGAGAAAATCAACACTTATCATTACTTCAATCCTCTTCCTCGCAGCTATTATATGGGGAATTGGGATATTTATGAATAGTGTGGATAGGTCTAATGAAGTAAGTGAAGACGATGAGGTCGAAGAAGATAAATTGGAAAGCAAAACAGAGAACTCTACTCTCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

492

Amino Acids

53.51

Weight (kDa)

8.92

Isoelectric Point (pI)

31.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 70 - 410 4e-40 Major Facilitator Superfamily
Sugar_tr PF00083 78 - 436 4.5e-14 Sugar (and other) transporter
MFS_4 PF06779 83 - 239 6.5e-07 Uncharacterised MFS-type transporter YbfB
OATP PF03137 134 - 368 3.8e-18 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1427
AccB7I CCANNNNNTGG 1 cut(s) 1283
AccI GTMKAC 1 cut(s) 532
AccII CGCG 2 cut(s) 143, 941
AciI CCGC 5 cut(s) 47, 381, 665, 941, 975
AclWI GGATC 2 cut(s) 1240, 1305
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 3 cut(s) 210, 502, 1146
AfiI CCNNNNNNNGG 2 cut(s) 911, 1283
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 7 cut(s) 242, 322, 722, 1035, 1045, 1236, 1341
AjnI CCWGG 1 cut(s) 786
AluBI AGCT 8 cut(s) 44, 165, 673, 749, 887, 1002, 1055, 1358
AluI AGCT 8 cut(s) 44, 165, 673, 749, 887, 1002, 1055, 1358
Alw21I GWGCWC 1 cut(s) 487
Alw26I GTCTC 3 cut(s) 1193, 1195, 1207
AlwI GGATC 2 cut(s) 1240, 1305
AlwNI CAGNNNCTG 1 cut(s) 206
AoxI GGCC 1 cut(s) 13
ApeKI GCWGC 6 cut(s) 461, 667, 670, 1055, 1076, 1355
ApoI RAATTY 3 cut(s) 210, 502, 1146
ArsI GACNNNNNNTTYG 2 cut(s) 935, 967
Asp700I GAANNNNTTC 1 cut(s) 503
AspLEI GCGC 1 cut(s) 1076
AspS9I GGNCC 1 cut(s) 902
AsuC2I CCSGG 1 cut(s) 78
AsuHPI GGTGA 2 cut(s) 449, 856
AvaII GGWCC 1 cut(s) 902
BalI TGGCCA 1 cut(s) 15
BbsI GAAGAC 2 cut(s) 625, 1425
Bbv12I GWGCWC 1 cut(s) 487
BbvI GCAGC 6 cut(s) 473, 679, 682, 1042, 1088, 1367
BccI CCATC 2 cut(s) 239, 585
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BciT130I CCWGG 1 cut(s) 788
BciVI GTATCC 1 cut(s) 563
BcnI CCSGG 1 cut(s) 78
BcoDI GTCTC 3 cut(s) 1193, 1195, 1207
BfaI CTAG 2 cut(s) 551, 1052
BfmI CTRYAG 2 cut(s) 198, 735
BfuI GTATCC 1 cut(s) 563
BisI GCNGC 6 cut(s) 462, 668, 671, 1056, 1077, 1356
BlsI GCNGC 6 cut(s) 463, 669, 672, 1057, 1078, 1357
Bme1390I CCNGG 2 cut(s) 78, 788
Bme18I GGWCC 1 cut(s) 902
BmgT120I GGNCC 1 cut(s) 902
BmiI GGNNCC 2 cut(s) 903, 1170
BmrFI CCNGG 2 cut(s) 78, 788
BmsI GCATC 3 cut(s) 169, 730, 1067
BoxI GACNNNNGTC 1 cut(s) 1219
BpiI GAAGAC 2 cut(s) 625, 1425
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpuEI CTTGAG 1 cut(s) 1231
BpuMI CCSGG 1 cut(s) 78
Bsa29I ATCGAT 1 cut(s) 474
BsaBI GATNNNNATC 1 cut(s) 1250
BsaI GGTCTC 2 cut(s) 1195, 1207
BsaWI WCCGGW 1 cut(s) 1171
BsaXI ACNNNNNCTCC 2 cut(s) 715, 745
Bsc4I CCNNNNNNNGG 2 cut(s) 911, 1283
Bse118I RCCGGY 2 cut(s) 49, 911
Bse1I ACTGG 1 cut(s) 211
Bse3DI GCAATG 2 cut(s) 630, 1192
Bse8I GATNNNNATC 1 cut(s) 1250
BseBI CCWGG 1 cut(s) 788
BseCI ATCGAT 1 cut(s) 474
BseGI GGATG 2 cut(s) 408, 596
BseJI GATNNNNATC 1 cut(s) 1250
BseLI CCNNNNNNNGG 2 cut(s) 911, 1283
BseMI GCAATG 2 cut(s) 630, 1192
BseNI ACTGG 1 cut(s) 211
BseRI GAGGAG 1 cut(s) 147
BseXI GCAGC 6 cut(s) 473, 679, 682, 1042, 1088, 1367
Bsh1236I CGCG 2 cut(s) 143, 941
BshFI GGCC 1 cut(s) 15
BshVI ATCGAT 1 cut(s) 474
BsiHKAI GWGCWC 1 cut(s) 487
BsiSI CCGG 5 cut(s) 29, 50, 77, 912, 1172
BslFI GGGAC 1 cut(s) 915
BslI CCNNNNNNNGG 2 cut(s) 911, 1283
BsmAI GTCTC 3 cut(s) 1193, 1195, 1207
BsmFI GGGAC 1 cut(s) 915
BsnI GGCC 1 cut(s) 15
Bso31I GGTCTC 2 cut(s) 1195, 1207
Bsp1286I GDGCHC 1 cut(s) 487
Bsp143I GATC 5 cut(s) 127, 138, 1012, 1245, 1297
Bsp68I TCGCGA 1 cut(s) 143
BspACI CCGC 5 cut(s) 47, 381, 665, 941, 975
BspANI GGCC 1 cut(s) 15
BspDI ATCGAT 1 cut(s) 474
BspFNI CGCG 2 cut(s) 143, 941
BspLI GGNNCC 2 cut(s) 903, 1170
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 2 cut(s) 1240, 1305
BspTNI GGTCTC 2 cut(s) 1195, 1207
BsrDI GCAATG 2 cut(s) 630, 1192
BsrFI RCCGGY 2 cut(s) 49, 911
BsrI ACTGG 1 cut(s) 211
BssAI RCCGGY 2 cut(s) 49, 911
BssMI GATC 5 cut(s) 127, 138, 1012, 1245, 1297
BssNAI GTATAC 1 cut(s) 533
Bst1107I GTATAC 1 cut(s) 533
Bst2UI CCWGG 1 cut(s) 788
Bst4CI ACNGT 4 cut(s) 355, 519, 727, 1240
Bst6I CTCTTC 1 cut(s) 1352
BstAPI GCANNNNNTGC 1 cut(s) 545
BstC8I GCNNGC 1 cut(s) 51
BstDEI CTNAG 2 cut(s) 699, 1255
BstF5I GGATG 2 cut(s) 408, 596
BstFNI CGCG 2 cut(s) 143, 941
BstHHI GCGC 1 cut(s) 1076
BstKTI GATC 5 cut(s) 130, 141, 1015, 1248, 1300
BstMAI GTCTC 3 cut(s) 1193, 1195, 1207
BstMBI GATC 5 cut(s) 127, 138, 1012, 1245, 1297
BstMWI GCNNNNNNNGC 6 cut(s) 50, 491, 545, 1124, 1133, 1166
BstNI CCWGG 1 cut(s) 788
BstNSI RCATGY 1 cut(s) 529
BstPAI GACNNNNGTC 1 cut(s) 1219
BstSCI CCNGG 2 cut(s) 76, 786
BstSFI CTRYAG 2 cut(s) 198, 735
BstUI CGCG 2 cut(s) 143, 941
BstV1I GCAGC 6 cut(s) 473, 679, 682, 1042, 1088, 1367
BstV2I GAAGAC 2 cut(s) 625, 1425
BstXI CCANNNNNNTGG 1 cut(s) 787
BstZ17I GTATAC 1 cut(s) 533
Bsu15I ATCGAT 1 cut(s) 474
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 1 cut(s) 15
BsuTUI ATCGAT 1 cut(s) 474
BtgZI GCGATG 2 cut(s) 1108, 1275
BtsCI GGATG 2 cut(s) 408, 596
BtsI GCAGTG 2 cut(s) 1089, 1143
BtsIMutI CAGTG 2 cut(s) 1089, 1143
BtuMI TCGCGA 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 51
CaiI CAGNNNCTG 1 cut(s) 206
CfoI GCGC 1 cut(s) 1076
Cfr10I RCCGGY 2 cut(s) 49, 911
Cfr13I GGNCC 1 cut(s) 902
ClaI ATCGAT 1 cut(s) 474
CsiI ACCWGGT 1 cut(s) 786
CviAII CATG 2 cut(s) 526, 946
DdeI CTNAG 2 cut(s) 699, 1255
DpnI GATC 5 cut(s) 129, 140, 1014, 1247, 1299
DpnII GATC 5 cut(s) 127, 138, 1012, 1245, 1297
DraI TTTAAA 2 cut(s) 331, 1305
DrdI GACNNNNNNGTC 1 cut(s) 1427
DseDI GACNNNNNNGTC 1 cut(s) 1427
EaeI YGGCCR 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 1352
EarI CTCTTC 1 cut(s) 1352
EciI GGCGGA 1 cut(s) 36
Eco31I GGTCTC 2 cut(s) 1195, 1207
Eco47I GGWCC 1 cut(s) 902
EcoRI GAATTC 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 786
FaeI CATG 2 cut(s) 529, 949
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FaqI GGGAC 1 cut(s) 915
FatI CATG 2 cut(s) 525, 945
FauI CCCGC 1 cut(s) 374
FauNDI CATATG 1 cut(s) 971
FblI GTMKAC 1 cut(s) 532
Fnu4HI GCNGC 6 cut(s) 462, 668, 671, 1056, 1077, 1356
FokI GGATG 2 cut(s) 415, 603
Fsp4HI GCNGC 6 cut(s) 462, 668, 671, 1056, 1077, 1356
FspBI CTAG 2 cut(s) 551, 1052
GlaI GCGC 1 cut(s) 1075
GluI GCNGC 6 cut(s) 462, 668, 671, 1056, 1077, 1356
HaeIII GGCC 1 cut(s) 15
HapII CCGG 5 cut(s) 29, 50, 77, 912, 1172
HhaI GCGC 1 cut(s) 1076
Hin1II CATG 2 cut(s) 529, 949
Hin6I GCGC 1 cut(s) 1074
HinP1I GCGC 1 cut(s) 1074
HincII GTYRAC 1 cut(s) 1177
HindII GTYRAC 1 cut(s) 1177
HindIII AAGCTT 1 cut(s) 747
HinfI GANTC 5 cut(s) 23, 73, 84, 318, 695
HpaI GTTAAC 1 cut(s) 1177
HpaII CCGG 5 cut(s) 29, 50, 77, 912, 1172
HphI GGTGA 2 cut(s) 449, 856
Hpy166II GTNNAC 2 cut(s) 533, 1177
Hpy188I TCNGA 1 cut(s) 419
Hpy188III TCNNGA 4 cut(s) 88, 142, 400, 1099
Hpy8I GTNNAC 2 cut(s) 533, 1177
HpyAV CCTTC 4 cut(s) 658, 1051, 1129, 1278
HpyCH4III ACNGT 4 cut(s) 355, 519, 727, 1240
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 7 cut(s) 200, 461, 494, 743, 1058, 1160, 1294
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 491, 545, 1124, 1133, 1166
HpyF3I CTNAG 2 cut(s) 699, 1255
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 2 cut(s) 529, 949
HspAI GCGC 1 cut(s) 1074
KroI GCCGGC 1 cut(s) 49
KroNI GCCGGC 1 cut(s) 51
KspAI GTTAAC 1 cut(s) 1177
Kzo9I GATC 5 cut(s) 127, 138, 1012, 1245, 1297
LmnI GCTCC 5 cut(s) 49, 490, 736, 884, 1174
Lsp1109I GCAGC 6 cut(s) 473, 679, 682, 1042, 1088, 1367
LweI GCATC 3 cut(s) 169, 730, 1067
MabI ACCWGGT 1 cut(s) 786
MaeI CTAG 2 cut(s) 551, 1052
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 3 cut(s) 88, 857, 1047
MalI GATC 5 cut(s) 129, 140, 1014, 1247, 1299
MboI GATC 5 cut(s) 127, 138, 1012, 1245, 1297
MhlI GDGCHC 1 cut(s) 487
MlsI TGGCCA 1 cut(s) 15
MluCI AATT 9 cut(s) 118, 210, 502, 756, 981, 1146, 1309, 1371, 1442
MluNI TGGCCA 1 cut(s) 15
MlyI GAGTC 1 cut(s) 67
MmeI TCCRAC 2 cut(s) 253, 325
MnlI CCTC 8 cut(s) 125, 433, 607, 718, 950, 1355, 1361, 1420
Mox20I TGGCCA 1 cut(s) 15
MreI CGCCGGCG 1 cut(s) 49
MroNI GCCGGC 1 cut(s) 49
MroXI GAANNNNTTC 1 cut(s) 503
MscI TGGCCA 1 cut(s) 15
Msp20I TGGCCA 1 cut(s) 15
MspI CCGG 5 cut(s) 29, 50, 77, 912, 1172
MspR9I CCNGG 2 cut(s) 78, 788
MvaI CCWGG 1 cut(s) 788
MvnI CGCG 2 cut(s) 143, 941
MwoI GCNNNNNNNGC 6 cut(s) 50, 491, 545, 1124, 1133, 1166
NaeI GCCGGC 1 cut(s) 51
NciI CCSGG 1 cut(s) 78
NdeI CATATG 1 cut(s) 971
NdeII GATC 5 cut(s) 127, 138, 1012, 1245, 1297
NgoMIV GCCGGC 1 cut(s) 49
NlaIII CATG 2 cut(s) 529, 949
NlaIV GGNNCC 2 cut(s) 903, 1170
NmeAIII GCCGAG 1 cut(s) 1050
NmuCI GTSAC 1 cut(s) 88
NruI TCGCGA 1 cut(s) 143
NspI RCATGY 1 cut(s) 529
PciI ACATGT 1 cut(s) 525
PcsI WCGNNNNNNNCGW 1 cut(s) 1428
PdiI GCCGGC 1 cut(s) 51
PdmI GAANNNNTTC 1 cut(s) 503
PfeI GAWTC 4 cut(s) 23, 84, 318, 695
PflMI CCANNNNNTGG 1 cut(s) 1283
PfoI TCCNGGA 1 cut(s) 76
PkrI GCNGC 6 cut(s) 463, 669, 672, 1057, 1078, 1357
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
PscI ACATGT 1 cut(s) 525
PshAI GACNNNNGTC 1 cut(s) 1219
Psp6I CCWGG 1 cut(s) 786
PspGI CCWGG 1 cut(s) 786
PspN4I GGNNCC 2 cut(s) 903, 1170
PspPI GGNCC 1 cut(s) 902
PstI CTGCAG 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 206
RruI TCGCGA 1 cut(s) 143
SatI GCNGC 6 cut(s) 462, 668, 671, 1056, 1077, 1356
Sau3AI GATC 5 cut(s) 127, 138, 1012, 1245, 1297
Sau96I GGNCC 1 cut(s) 902
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 2 cut(s) 78, 788
SduI GDGCHC 1 cut(s) 487
SexAI ACCWGGT 1 cut(s) 786
SfaNI GCATC 3 cut(s) 169, 730, 1067
SfcI CTRYAG 2 cut(s) 198, 735
SgrAI CRCCGGYG 1 cut(s) 49
SinI GGWCC 1 cut(s) 902
SmlI CTYRAG 1 cut(s) 1210
SmoI CTYRAG 1 cut(s) 1210
Sse9I AATT 9 cut(s) 118, 210, 502, 756, 981, 1146, 1309, 1371, 1442
SsiI CCGC 5 cut(s) 47, 381, 665, 941, 975
SspI AATATT 1 cut(s) 297
SspMI CTAG 2 cut(s) 551, 1052
StyD4I CCNGG 2 cut(s) 76, 786
TaaI ACNGT 4 cut(s) 355, 519, 727, 1240
TaiI ACGT 1 cut(s) 126
TaqI TCGA 3 cut(s) 130, 474, 1431
TasI AATT 9 cut(s) 118, 210, 502, 756, 981, 1146, 1309, 1371, 1442
TfiI GAWTC 4 cut(s) 23, 84, 318, 695
TscAI CASTG 2 cut(s) 1096, 1143
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 6 cut(s) 461, 667, 670, 1055, 1076, 1355
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 5 cut(s) 947, 1111, 1118, 1400, 1422
TspRI CASTG 2 cut(s) 1096, 1143
Van91I CCANNNNNTGG 1 cut(s) 1283
VpaK11BI GGWCC 1 cut(s) 902
XapI RAATTY 3 cut(s) 210, 502, 1146
XceI RCATGY 1 cut(s) 529
XmiI GTMKAC 1 cut(s) 532
XmnI GAANNNNTTC 1 cut(s) 503
XspI CTAG 2 cut(s) 551, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.