Rh2BG224300

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001699
Physical Location & Seq
Reverse (-)
21744350 .. 21750260
5911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG224300.1

Sequence Viewer

Length: 1506 bp
ATGATCATGGCACAACCACCACAATCACCGTCAAAATTCGCCCAATTCCCCAATCCTAATTCCCTTCCACCGATGACAAAGAACCCCAAGGCAGCAATCTCATCTCCAAAGCCTTCCTGGTTCTCCCCCGAAAGGTTGCTGTTAATCCTGTGTCTGGTTAACTTAATAAATTATATTGATCGAGGAGCAATAGCCAGCAATGGGGTCAATGGAAGTATTGGGATTTGCGATGAGAGCGGAATTTGCCATGCCGGTACCGGAATTCAGGGGGATTTTAAATTGAACAACTTTCAAGATGGTGTTCTATCATCTGCATTCATGGTTGGGCTTCTCGTGGCCTCACCCATCTTTGCATCCTTGGCAAAGAGCCACAATCCTTTTAGGCTGATTGGAGTTGGGTTATCTATTTGGACATTTGCCACAGCTGGGTGTGGTAGTTCATTTAATTTCTGGTCCATTGCAATATGTCGCATGCTGGTTGGAGTCGGTGAGGCATCTTTCATAAGTCTTGCAGCTCCGTTCATTGATGACCAAGCCCCTGCAGATCAGGTGGGAGAGAGCATCAATTGGCGTTGTGCATTCTGGGGAGAGGCCCTTCTAATGCTTCCCTTTGCTGTCTTGGGTTTTGCGGTGAAACCATTGCAGCTGAAAGGTTTACCTCCTACTGAATCGACAGAGCTTTTGGAATCTATTGAAACTAGTCCTTTAATAGAAGAATCAGAGATCCCAAATATAAATGGTCGTGTGACAGTTGACTGTGAAGAGACTTCTTGCTCATGCCCTACAACCAAATTTTTGGACCAAGTTTTAAGCTTCTCAAAGGATTTGAAAGTACTTCTGCTTGATAAAGTTTACATTGTTAATGTATTAGGTTATGCGTCATACAACTTTGTCATTGGAACCTACTCATATTGGGGGCCAAAGGCTGGTTATAACATTTACCATATGAGTAATCCAGATCTGATGTTCGGAGGTATTACTATTCTCTGTGGAATTCTAGGAACTTTAACTGGAGGGCTCGTCCTTGATGCAGTGGGTGCTACAATTCACAATGCTTTTAAGCTTCTTGCTGGAGCGACAATGCTAGGTGCAATATTTTGCTTCAGTGCGTTTTGTGCAAAGAGCCTGCACATCTTTGTAGTTTTTTTCTCACTGGGGGAGCTACTTCTGTTTTCAACACAGGCTCCAGTAAATTATGTGTCTCTGCACTGCGTCAAACCAGGGCTAAGGCCATTGTCTATGGCTATCTCAACTCTTTCAATCCACATATTTGGTGATGTTCCTTCCTCGCCTCTTGTCGGAATCATTCAGGATTATGTTCACAATTGGAGGGAAACGACTCTAATCTTTACCTCTGTCTACTTTGTTGCATCAGGAATATGGTTCATAGGGATCTTCCTTAATAGTGTGGACAAGTTTGAGGAAGATGAAGAAGAAGAAGTCTCTACATTAGTTCAAGCCAACAAAGAACCATTGCTTGAAGAGCACCACTTAAGGAAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

501

Amino Acids

54.34

Weight (kDa)

5.11

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 87 - 185 1.3e-10 Major Facilitator Superfamily
MFS_1 PF07690 186 - 420 8.3e-10 Major Facilitator Superfamily
OATP PF03137 259 - 371 1.1e-08 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 371 - 438 1.5e-07 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 933
Acc65I GGTACC 1 cut(s) 254
AccB1I GGYRCC 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 926
AccBSI CCGCTC 1 cut(s) 237
AccI GTMKAC 1 cut(s) 1359
AciI CCGC 2 cut(s) 237, 629
AclWI GGATC 2 cut(s) 718, 1400
AcsI RAATTY 5 cut(s) 35, 240, 261, 791, 993
AcuI CTGAAG 1 cut(s) 1087
AfaI GTAC 2 cut(s) 256, 834
AfiI CCNNNNNNNGG 6 cut(s) 132, 154, 201, 426, 926, 1298
AflII CTTAAG 1 cut(s) 1492
AgsI TTSAA 8 cut(s) 283, 293, 695, 829, 1176, 1260, 1457, 1481
AhlI ACTAGT 1 cut(s) 698
AjnI CCWGG 2 cut(s) 116, 1219
AluBI AGCT 7 cut(s) 425, 515, 646, 679, 813, 1063, 1162
AluI AGCT 7 cut(s) 425, 515, 646, 679, 813, 1063, 1162
Alw21I GWGCWC 1 cut(s) 1488
Alw26I GTCTC 3 cut(s) 758, 1206, 1447
AlwI GGATC 2 cut(s) 718, 1400
AoxI GGCC 4 cut(s) 336, 591, 917, 1229
ApeKI GCWGC 3 cut(s) 92, 512, 643
ApoI RAATTY 5 cut(s) 35, 240, 261, 791, 993
ArsI GACNNNNNNTTYG 2 cut(s) 664, 696
Asp718I GGTACC 1 cut(s) 254
AspS9I GGNCC 4 cut(s) 453, 592, 799, 917
AsuHPI GGTGA 5 cut(s) 18, 333, 500, 643, 1286
AvaII GGWCC 2 cut(s) 453, 799
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 1 cut(s) 1020
BauI CACGAG 1 cut(s) 332
Bbv12I GWGCWC 1 cut(s) 1488
BbvI GCAGC 3 cut(s) 104, 524, 655
BccI CCATC 2 cut(s) 290, 353
BciT130I CCWGG 2 cut(s) 118, 1221
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 3 cut(s) 758, 1206, 1447
BcuI ACTAGT 1 cut(s) 698
BfaI CTAG 4 cut(s) 699, 998, 1085, 1504
BfmI CTRYAG 1 cut(s) 540
BfrI CTTAAG 1 cut(s) 1492
BglII AGATCT 1 cut(s) 958
BisI GCNGC 3 cut(s) 93, 513, 644
BlsI GCNGC 3 cut(s) 94, 514, 645
BmcAI AGTACT 1 cut(s) 834
Bme1390I CCNGG 2 cut(s) 118, 1221
Bme18I GGWCC 2 cut(s) 453, 799
BmgT120I GGNCC 4 cut(s) 453, 592, 799, 917
BmiI GGNNCC 4 cut(s) 256, 901, 918, 1185
BmrFI CCNGG 2 cut(s) 118, 1221
BmrI ACTGGG 1 cut(s) 1163
BmsI GCATC 5 cut(s) 362, 503, 570, 1018, 1379
BmuI ACTGGG 1 cut(s) 1163
BpmI CTGGAG 3 cut(s) 1032, 1092, 1170
Bpu10I CCTNAGC 1 cut(s) 1226
BsaJI CCNNGG 3 cut(s) 87, 357, 1220
BsaWI WCCGGW 1 cut(s) 257
BsaXI ACNNNNNCTCC 4 cut(s) 1005, 1035, 1168, 1198
Bsc4I CCNNNNNNNGG 6 cut(s) 132, 154, 201, 426, 926, 1298
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 3 cut(s) 1015, 1158, 1187
Bse3DI GCAATG 4 cut(s) 205, 456, 638, 1472
BseBI CCWGG 2 cut(s) 118, 1221
BseDI CCNNGG 3 cut(s) 87, 357, 1220
BseGI GGATG 1 cut(s) 353
BseLI CCNNNNNNNGG 6 cut(s) 132, 154, 201, 426, 926, 1298
BseMI GCAATG 4 cut(s) 205, 456, 638, 1472
BseNI ACTGG 3 cut(s) 1015, 1158, 1187
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 3 cut(s) 104, 524, 655
BseYI CCCAGC 1 cut(s) 425
BsgI GTGCAG 2 cut(s) 1112, 1190
BshFI GGCC 4 cut(s) 338, 593, 919, 1231
BshNI GGYRCC 1 cut(s) 254
BsiHKAI GWGCWC 1 cut(s) 1488
BsiSI CCGG 2 cut(s) 252, 258
BslI CCNNNNNNNGG 6 cut(s) 132, 154, 201, 426, 926, 1298
BsmAI GTCTC 3 cut(s) 758, 1206, 1447
BsmI GAATGC 2 cut(s) 314, 578
BsnI GGCC 4 cut(s) 338, 593, 919, 1231
Bsp1286I GDGCHC 2 cut(s) 1020, 1488
Bsp143I GATC 6 cut(s) 3, 178, 544, 723, 958, 1392
BspACI CCGC 2 cut(s) 237, 629
BspANI GGCC 4 cut(s) 338, 593, 919, 1231
BspLI GGNNCC 4 cut(s) 256, 901, 918, 1185
BspMAI CTGCAG 1 cut(s) 544
BspPI GGATC 2 cut(s) 718, 1400
BspQI GCTCTTC 1 cut(s) 1476
BspT107I GGYRCC 1 cut(s) 254
BspTI CTTAAG 1 cut(s) 1492
BsrBI CCGCTC 1 cut(s) 237
BsrDI GCAATG 4 cut(s) 205, 456, 638, 1472
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 3 cut(s) 1015, 1158, 1187
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 3 cut(s) 87, 357, 1220
BssMI GATC 6 cut(s) 3, 178, 544, 723, 958, 1392
BssSI CACGAG 1 cut(s) 332
BssT1I CCWWGG 2 cut(s) 87, 357
Bst2BI CACGAG 1 cut(s) 332
Bst2UI CCWGG 2 cut(s) 118, 1221
Bst4CI ACNGT 3 cut(s) 30, 751, 758
Bst6I CTCTTC 2 cut(s) 756, 1476
BstAFI CTTAAG 1 cut(s) 1492
BstAPI GCANNNNNTGC 1 cut(s) 1037
BstC8I GCNNGC 3 cut(s) 196, 473, 1127
BstDEI CTNAG 1 cut(s) 1226
BstF5I GGATG 1 cut(s) 353
BstKTI GATC 6 cut(s) 6, 181, 547, 726, 961, 1395
BstMAI GTCTC 3 cut(s) 758, 1206, 1447
BstMBI GATC 6 cut(s) 3, 178, 544, 723, 958, 1392
BstMWI GCNNNNNNNGC 6 cut(s) 234, 243, 359, 1037, 1115, 1483
BstNI CCWGG 2 cut(s) 118, 1221
BstNSI RCATGY 1 cut(s) 475
BstSCI CCNGG 2 cut(s) 116, 1219
BstSFI CTRYAG 1 cut(s) 540
BstV1I GCAGC 3 cut(s) 104, 524, 655
BstX2I RGATCY 3 cut(s) 723, 958, 1392
BstXI CCANNNNNNTGG 2 cut(s) 796, 1271
BstYI RGATCY 3 cut(s) 723, 958, 1392
BsuRI GGCC 4 cut(s) 338, 593, 919, 1231
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 1 cut(s) 353
BtsI GCAGTG 2 cut(s) 1038, 1207
BtsIMutI CAGTG 4 cut(s) 1038, 1111, 1151, 1207
Cac8I GCNNGC 3 cut(s) 196, 473, 1127
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 4 cut(s) 453, 592, 799, 917
CseI GACGC 2 cut(s) 867, 1201
Csp6I GTAC 2 cut(s) 255, 833
CviAII CATG 5 cut(s) 7, 248, 319, 472, 777
CviQI GTAC 2 cut(s) 255, 833
DdeI CTNAG 1 cut(s) 1226
DpnI GATC 6 cut(s) 5, 180, 546, 725, 960, 1394
DpnII GATC 6 cut(s) 3, 178, 544, 723, 958, 1392
DraI TTTAAA 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 756, 1476
EarI CTCTTC 2 cut(s) 756, 1476
Eco130I CCWWGG 2 cut(s) 87, 357
Eco24I GRGCYC 1 cut(s) 1020
Eco47I GGWCC 2 cut(s) 453, 799
Eco57I CTGAAG 1 cut(s) 1087
EcoO109I RGGNCCY 1 cut(s) 592
EcoRI GAATTC 2 cut(s) 261, 993
EcoRII CCWGG 2 cut(s) 116, 1219
EcoT14I CCWWGG 2 cut(s) 87, 357
EcoT38I GRGCYC 1 cut(s) 1020
ErhI CCWWGG 2 cut(s) 87, 357
FaeI CATG 5 cut(s) 10, 251, 322, 475, 780
FatI CATG 5 cut(s) 6, 247, 318, 471, 776
FauNDI CATATG 1 cut(s) 945
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 1359
Fnu4HI GCNGC 3 cut(s) 93, 513, 644
FokI GGATG 1 cut(s) 340
FriOI GRGCYC 1 cut(s) 1020
Fsp4HI GCNGC 3 cut(s) 93, 513, 644
FspBI CTAG 4 cut(s) 699, 998, 1085, 1504
GluI GCNGC 3 cut(s) 93, 513, 644
GsaI CCCAGC 1 cut(s) 429
GsuI CTGGAG 3 cut(s) 1032, 1092, 1170
HaeIII GGCC 4 cut(s) 338, 593, 919, 1231
HapII CCGG 2 cut(s) 252, 258
HgaI GACGC 2 cut(s) 867, 1201
Hin1II CATG 5 cut(s) 10, 251, 322, 475, 780
HincII GTYRAC 2 cut(s) 160, 754
HindII GTYRAC 2 cut(s) 160, 754
HindIII AAGCTT 2 cut(s) 811, 1061
HinfI GANTC 6 cut(s) 483, 668, 686, 716, 1302, 1339
HpaI GTTAAC 1 cut(s) 160
HpaII CCGG 2 cut(s) 252, 258
HphI GGTGA 5 cut(s) 18, 333, 500, 643, 1286
Hpy166II GTNNAC 7 cut(s) 160, 656, 754, 853, 1321, 1360, 1411
Hpy188I TCNGA 4 cut(s) 721, 963, 971, 1301
Hpy188III TCNNGA 4 cut(s) 293, 956, 1310, 1374
Hpy8I GTNNAC 7 cut(s) 160, 656, 754, 853, 1321, 1360, 1411
HpyAV CCTTC 5 cut(s) 74, 123, 605, 1293, 1492
HpyCH4III ACNGT 3 cut(s) 30, 751, 758
HpyF10VI GCNNNNNNNGC 6 cut(s) 234, 243, 359, 1037, 1115, 1483
HpyF3I CTNAG 1 cut(s) 1226
Hsp92II CATG 5 cut(s) 10, 251, 322, 475, 780
KpnI GGTACC 1 cut(s) 258
Ksp22I TGATCA 1 cut(s) 3
KspAI GTTAAC 1 cut(s) 160
Kzo9I GATC 6 cut(s) 3, 178, 544, 723, 958, 1392
LguI GCTCTTC 1 cut(s) 1476
LmnI GCTCC 5 cut(s) 185, 520, 1073, 1159, 1189
Lsp1109I GCAGC 3 cut(s) 104, 524, 655
LweI GCATC 5 cut(s) 362, 503, 570, 1018, 1379
MaeI CTAG 4 cut(s) 699, 998, 1085, 1504
MaeIII GTNAC 1 cut(s) 745
MalI GATC 6 cut(s) 5, 180, 546, 725, 960, 1394
MbiI CCGCTC 1 cut(s) 237
MboI GATC 6 cut(s) 3, 178, 544, 723, 958, 1392
MboII GAAGA 8 cut(s) 725, 773, 1387, 1436, 1442, 1445, 1448, 1493
MfeI CAATTG 2 cut(s) 565, 1324
MflI RGATCY 3 cut(s) 723, 958, 1392
MhlI GDGCHC 2 cut(s) 1020, 1488
MlyI GAGTC 2 cut(s) 492, 1333
MmeI TCCRAC 2 cut(s) 460, 1279
MspA1I CMGCKG 2 cut(s) 425, 646
MspCI CTTAAG 1 cut(s) 1492
MspI CCGG 2 cut(s) 252, 258
MspR9I CCNGG 2 cut(s) 118, 1221
MunI CAATTG 2 cut(s) 565, 1324
Mva1269I GAATGC 2 cut(s) 314, 578
MvaI CCWGG 2 cut(s) 118, 1221
MwoI GCNNNNNNNGC 6 cut(s) 234, 243, 359, 1037, 1115, 1483
NdeI CATATG 1 cut(s) 945
NdeII GATC 6 cut(s) 3, 178, 544, 723, 958, 1392
NlaIII CATG 5 cut(s) 10, 251, 322, 475, 780
NlaIV GGNNCC 4 cut(s) 256, 901, 918, 1185
NmuCI GTSAC 1 cut(s) 745
NspI RCATGY 1 cut(s) 475
PaeI GCATGC 1 cut(s) 475
PciSI GCTCTTC 1 cut(s) 1476
PctI GAATGC 2 cut(s) 314, 578
PfeI GAWTC 4 cut(s) 668, 686, 716, 1302
PflMI CCANNNNNTGG 1 cut(s) 926
PkrI GCNGC 3 cut(s) 94, 514, 645
PleI GAGTC 2 cut(s) 491, 1333
PpsI GAGTC 2 cut(s) 491, 1333
PsiI TTATAA 1 cut(s) 933
Psp6I CCWGG 2 cut(s) 116, 1219
PspFI CCCAGC 1 cut(s) 425
PspGI CCWGG 2 cut(s) 116, 1219
PspN4I GGNNCC 4 cut(s) 256, 901, 918, 1185
PspPI GGNCC 4 cut(s) 453, 592, 799, 917
PstI CTGCAG 1 cut(s) 544
PsuI RGATCY 3 cut(s) 723, 958, 1392
PvuII CAGCTG 2 cut(s) 425, 646
RsaI GTAC 2 cut(s) 256, 834
RsaNI GTAC 2 cut(s) 255, 833
SapI GCTCTTC 1 cut(s) 1476
SatI GCNGC 3 cut(s) 93, 513, 644
Sau3AI GATC 6 cut(s) 3, 178, 544, 723, 958, 1392
Sau96I GGNCC 4 cut(s) 453, 592, 799, 917
ScaI AGTACT 1 cut(s) 834
SchI GAGTC 2 cut(s) 492, 1333
ScrFI CCNGG 2 cut(s) 118, 1221
SduI GDGCHC 2 cut(s) 1020, 1488
SfaNI GCATC 5 cut(s) 362, 503, 570, 1018, 1379
SfcI CTRYAG 1 cut(s) 540
SinI GGWCC 2 cut(s) 453, 799
SmlI CTYRAG 1 cut(s) 1492
SmoI CTYRAG 1 cut(s) 1492
SpeI ACTAGT 1 cut(s) 698
SphI GCATGC 1 cut(s) 475
SsiI CCGC 2 cut(s) 237, 629
SspI AATATT 1 cut(s) 1095
SspMI CTAG 4 cut(s) 699, 998, 1085, 1504
StyD4I CCNGG 2 cut(s) 116, 1219
StyI CCWWGG 2 cut(s) 87, 357
TaaI ACNGT 3 cut(s) 30, 751, 758
TaqI TCGA 2 cut(s) 181, 671
TatI WGTACW 1 cut(s) 832
TfiI GAWTC 4 cut(s) 668, 686, 716, 1302
TscAI CASTG 4 cut(s) 1038, 1111, 1158, 1214
TseFI GTSAC 1 cut(s) 745
TseI GCWGC 3 cut(s) 92, 512, 643
Tsp45I GTSAC 1 cut(s) 745
TspDTI ATGAA 6 cut(s) 307, 429, 490, 511, 1375, 1443
TspGWI ACGGA 1 cut(s) 507
TspRI CASTG 4 cut(s) 1038, 1111, 1158, 1214
Van91I CCANNNNNTGG 1 cut(s) 926
Vha464I CTTAAG 1 cut(s) 1492
VpaK11BI GGWCC 2 cut(s) 453, 799
XapI RAATTY 5 cut(s) 35, 240, 261, 791, 993
XceI RCATGY 1 cut(s) 475
XcmI CCANNNNNNNNNTGG 1 cut(s) 114
XmiI GTMKAC 1 cut(s) 1359
XspI CTAG 4 cut(s) 699, 998, 1085, 1504
ZrmI AGTACT 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.