Rh2CG216300

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001699
Physical Location & Seq
Reverse (-)
21269697 .. 21274868
5172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG216300.1

Sequence Viewer

Length: 1263 bp
ATGGTTGGGCTTCTCGTGGCCTCACCCATCTTTGCATCCTTGGCAAAGAGCCACAATCCTTTTAGGCTGATTGGAGTTGGGTTATCTATTTGGACATTTGCCACAGCTGGGTGTGGTAGTTCATTTAATTTCTGGTCCATTGCAATATGTCGCATGCTGGTTGGAGTCGGTGAGGCATCTTTCATAAGTCTTGCAGCTCCGTTCATTGATGACCATGCCCCTGCAGATCAGAAATCAGCATGGCTGGCAATGTTTTACATGTGTATACCAACTGGAGTCGCAATAGGCTATGTTTACGGCGGATTTGTGGGAGAGAGCATCAATTGGCGTTGTGCATTCTGGGGAGAGGCCCTTCTAATGCTTCCCTTTGCTGTCTTGGGTTTTGCGGTGAAACCATTGCAGCTGAAAGGTTTACCTCCTACTGAATCGACAGAGCTTTTGGAATCTATTGAAACTAGTCCTTTAATAGAAGAATCAGAGATCCCAAATATAAATGGTCATGTGACAGTTGACTGCAATGAGACTTCTTGCTCATGCCCTACAACCAAATTTTTGGACCAAGTTTCAAGCTTCTCAAAGGATTTGAAAGTACTTCTGCTTGATAAAGTTTACATTGTTAATGTATTAGGTTATGTATCATACAACTTTGTCATTGGAACCTACTCATACTGGGGGCCAAAGGCTGGTTATAACATTTACCATATGAGTAATCCAGATCTGATGTTCGGAGGTATTACTATTCTCTGTGGAATTCTAGGAACTTTAACTGGAGGGCTCGTCCTTGATGCAGTGGGTGCTACAATTCACAATGCTTTTAAGCTTCTTGCTGGAGCGACAATGCTAGGTGCAATATTTTGCTTCAGTGCGTTTTGTGCAAAGAGCCTGCACATCTTTGTAGTTTTTTTCTCACTGGGGGAGCTACTTCTGTTTTCAACACAGGCTCCAGTAAATTATGTGTCTCTGCACTGCGTCAAACCAGGGCTAAGGCCATTGTCTATGGCTATCTCAACTCTTTCAATCCACATATTTGGTGATGTTCCTTCCTCGCCTCTTGTCGGAATCGTTCAGGATTATGTTCACAATTGGAGGGAAACGACTCTAATCTTTACCTCTGTCTACTTTGCTGCATCAGGAATATGGTTCATAGGGATCTTCCTTAATAGTGTGGACAAGTTTGAGGAAGATGATGAAGAAGAAGTCTCTACATTAGTTCAAGCCAACAAAGAACCATTGCTTGAAGAGCACCACTTAAGGAAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

420

Amino Acids

45.73

Weight (kDa)

5.1

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 2 - 339 8.9e-28 Major Facilitator Superfamily
OATP PF03137 44 - 290 2.5e-17 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 289 - 357 1.2e-07 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 690
AccB7I CCANNNNNTGG 1 cut(s) 683
AccI GTMKAC 2 cut(s) 265, 1116
AciI CCGC 2 cut(s) 300, 386
AclWI GGATC 2 cut(s) 475, 1157
AcsI RAATTY 2 cut(s) 548, 750
AcuI CTGAAG 1 cut(s) 844
AfaI GTAC 1 cut(s) 591
AfiI CCNNNNNNNGG 3 cut(s) 108, 683, 1055
AflII CTTAAG 1 cut(s) 1249
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 7 cut(s) 452, 567, 586, 933, 1017, 1214, 1238
AhlI ACTAGT 1 cut(s) 455
AjnI CCWGG 1 cut(s) 976
AluBI AGCT 7 cut(s) 107, 197, 403, 436, 570, 820, 919
AluI AGCT 7 cut(s) 107, 197, 403, 436, 570, 820, 919
Alw21I GWGCWC 1 cut(s) 1245
Alw26I GTCTC 3 cut(s) 515, 963, 1204
AlwI GGATC 2 cut(s) 475, 1157
AoxI GGCC 4 cut(s) 18, 348, 674, 986
ApeKI GCWGC 3 cut(s) 194, 400, 1124
ApoI RAATTY 2 cut(s) 548, 750
ArsI GACNNNNNNTTYG 2 cut(s) 421, 453
AspS9I GGNCC 4 cut(s) 135, 349, 556, 674
AsuHPI GGTGA 4 cut(s) 15, 182, 400, 1043
AvaII GGWCC 2 cut(s) 135, 556
BanII GRGCYC 1 cut(s) 777
BauI CACGAG 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 1245
BbvI GCAGC 3 cut(s) 206, 412, 1111
BccI CCATC 1 cut(s) 35
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 1 cut(s) 978
BcoDI GTCTC 3 cut(s) 515, 963, 1204
BcuI ACTAGT 1 cut(s) 455
BfaI CTAG 4 cut(s) 456, 755, 842, 1261
BfmI CTRYAG 1 cut(s) 222
BfrI CTTAAG 1 cut(s) 1249
BglII AGATCT 1 cut(s) 715
BisI GCNGC 3 cut(s) 195, 401, 1125
BlsI GCNGC 3 cut(s) 196, 402, 1126
BmcAI AGTACT 1 cut(s) 591
Bme1390I CCNGG 1 cut(s) 978
Bme18I GGWCC 2 cut(s) 135, 556
BmgT120I GGNCC 4 cut(s) 135, 349, 556, 674
BmiI GGNNCC 3 cut(s) 658, 675, 942
BmrFI CCNGG 1 cut(s) 978
BmrI ACTGGG 2 cut(s) 679, 920
BmsI GCATC 5 cut(s) 44, 185, 327, 775, 1136
BmuI ACTGGG 2 cut(s) 679, 920
BpmI CTGGAG 4 cut(s) 294, 789, 849, 927
Bpu10I CCTNAGC 1 cut(s) 983
BsaJI CCNNGG 2 cut(s) 39, 977
BsaXI ACNNNNNCTCC 4 cut(s) 762, 792, 925, 955
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 683, 1055
Bse1I ACTGG 5 cut(s) 277, 674, 772, 915, 944
Bse3DI GCAATG 5 cut(s) 138, 255, 395, 523, 1229
BseBI CCWGG 1 cut(s) 978
BseDI CCNNGG 2 cut(s) 39, 977
BseGI GGATG 1 cut(s) 35
BseLI CCNNNNNNNGG 3 cut(s) 108, 683, 1055
BseMI GCAATG 5 cut(s) 138, 255, 395, 523, 1229
BseNI ACTGG 5 cut(s) 277, 674, 772, 915, 944
BseXI GCAGC 3 cut(s) 206, 412, 1111
BseYI CCCAGC 1 cut(s) 107
BsgI GTGCAG 2 cut(s) 869, 947
BshFI GGCC 4 cut(s) 20, 350, 676, 988
BsiHKAI GWGCWC 1 cut(s) 1245
BslI CCNNNNNNNGG 3 cut(s) 108, 683, 1055
BsmAI GTCTC 3 cut(s) 515, 963, 1204
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 4 cut(s) 20, 350, 676, 988
Bsp1286I GDGCHC 2 cut(s) 777, 1245
Bsp143I GATC 4 cut(s) 226, 480, 715, 1149
BspACI CCGC 2 cut(s) 300, 386
BspANI GGCC 4 cut(s) 20, 350, 676, 988
BspLI GGNNCC 3 cut(s) 658, 675, 942
BspMAI CTGCAG 1 cut(s) 226
BspPI GGATC 2 cut(s) 475, 1157
BspQI GCTCTTC 1 cut(s) 1233
BspTI CTTAAG 1 cut(s) 1249
BsrDI GCAATG 5 cut(s) 138, 255, 395, 523, 1229
BsrI ACTGG 5 cut(s) 277, 674, 772, 915, 944
BssECI CCNNGG 2 cut(s) 39, 977
BssMI GATC 4 cut(s) 226, 480, 715, 1149
BssNAI GTATAC 1 cut(s) 266
BssSI CACGAG 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 39
Bst1107I GTATAC 1 cut(s) 266
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 1 cut(s) 978
Bst4CI ACNGT 1 cut(s) 508
Bst6I CTCTTC 1 cut(s) 1233
BstAFI CTTAAG 1 cut(s) 1249
BstAPI GCANNNNNTGC 1 cut(s) 794
BstC8I GCNNGC 3 cut(s) 155, 246, 884
BstDEI CTNAG 1 cut(s) 983
BstF5I GGATG 1 cut(s) 35
BstKTI GATC 4 cut(s) 229, 483, 718, 1152
BstMAI GTCTC 3 cut(s) 515, 963, 1204
BstMBI GATC 4 cut(s) 226, 480, 715, 1149
BstMWI GCNNNNNNNGC 5 cut(s) 41, 245, 794, 872, 1240
BstNI CCWGG 1 cut(s) 978
BstNSI RCATGY 2 cut(s) 157, 262
BstSCI CCNGG 1 cut(s) 976
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 3 cut(s) 206, 412, 1111
BstX2I RGATCY 3 cut(s) 480, 715, 1149
BstXI CCANNNNNNTGG 2 cut(s) 553, 1028
BstYI RGATCY 3 cut(s) 480, 715, 1149
BstZ17I GTATAC 1 cut(s) 266
BsuRI GGCC 4 cut(s) 20, 350, 676, 988
BtsCI GGATG 1 cut(s) 35
BtsI GCAGTG 2 cut(s) 795, 964
BtsIMutI CAGTG 4 cut(s) 795, 868, 908, 964
Cac8I GCNNGC 3 cut(s) 155, 246, 884
Cfr13I GGNCC 4 cut(s) 135, 349, 556, 674
CseI GACGC 1 cut(s) 958
Csp6I GTAC 1 cut(s) 590
CviAII CATG 6 cut(s) 154, 215, 240, 259, 500, 534
CviQI GTAC 1 cut(s) 590
DdeI CTNAG 1 cut(s) 983
DpnI GATC 4 cut(s) 228, 482, 717, 1151
DpnII GATC 4 cut(s) 226, 480, 715, 1149
Eam1104I CTCTTC 1 cut(s) 1233
EarI CTCTTC 1 cut(s) 1233
EciI GGCGGA 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 777
Eco47I GGWCC 2 cut(s) 135, 556
Eco57I CTGAAG 1 cut(s) 844
EcoO109I RGGNCCY 1 cut(s) 349
EcoRI GAATTC 1 cut(s) 750
EcoRII CCWGG 1 cut(s) 976
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 777
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 6 cut(s) 157, 218, 243, 262, 503, 537
FatI CATG 6 cut(s) 153, 214, 239, 258, 499, 533
FauNDI CATATG 1 cut(s) 702
FblI GTMKAC 2 cut(s) 265, 1116
Fnu4HI GCNGC 3 cut(s) 195, 401, 1125
FokI GGATG 1 cut(s) 22
FriOI GRGCYC 1 cut(s) 777
Fsp4HI GCNGC 3 cut(s) 195, 401, 1125
FspBI CTAG 4 cut(s) 456, 755, 842, 1261
GluI GCNGC 3 cut(s) 195, 401, 1125
GsaI CCCAGC 1 cut(s) 111
GsuI CTGGAG 4 cut(s) 294, 789, 849, 927
HaeIII GGCC 4 cut(s) 20, 350, 676, 988
HgaI GACGC 1 cut(s) 958
Hin1II CATG 6 cut(s) 157, 218, 243, 262, 503, 537
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 2 cut(s) 568, 818
HinfI GANTC 7 cut(s) 165, 276, 425, 443, 473, 1059, 1096
HphI GGTGA 4 cut(s) 15, 182, 400, 1043
Hpy166II GTNNAC 8 cut(s) 266, 295, 413, 511, 610, 1078, 1117, 1168
Hpy188I TCNGA 5 cut(s) 231, 478, 720, 728, 1058
Hpy188III TCNNGA 3 cut(s) 713, 1067, 1131
Hpy8I GTNNAC 8 cut(s) 266, 295, 413, 511, 610, 1078, 1117, 1168
HpyAV CCTTC 3 cut(s) 362, 1050, 1249
HpyCH4III ACNGT 1 cut(s) 508
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 245, 794, 872, 1240
HpyF3I CTNAG 1 cut(s) 983
Hsp92II CATG 6 cut(s) 157, 218, 243, 262, 503, 537
Kzo9I GATC 4 cut(s) 226, 480, 715, 1149
LguI GCTCTTC 1 cut(s) 1233
LmnI GCTCC 4 cut(s) 202, 830, 916, 946
Lsp1109I GCAGC 3 cut(s) 206, 412, 1111
LweI GCATC 5 cut(s) 44, 185, 327, 775, 1136
MaeI CTAG 4 cut(s) 456, 755, 842, 1261
MaeIII GTNAC 1 cut(s) 502
MalI GATC 4 cut(s) 228, 482, 717, 1151
MboI GATC 4 cut(s) 226, 480, 715, 1149
MboII GAAGA 6 cut(s) 482, 1144, 1193, 1202, 1205, 1250
MfeI CAATTG 2 cut(s) 322, 1081
MflI RGATCY 3 cut(s) 480, 715, 1149
MhlI GDGCHC 2 cut(s) 777, 1245
MluCI AATT 7 cut(s) 127, 322, 548, 750, 801, 949, 1081
MlyI GAGTC 3 cut(s) 174, 285, 1090
MmeI TCCRAC 2 cut(s) 142, 1036
MseI TTAA 7 cut(s) 126, 464, 618, 764, 816, 1158, 1250
MspA1I CMGCKG 2 cut(s) 107, 403
MspCI CTTAAG 1 cut(s) 1249
MspR9I CCNGG 1 cut(s) 978
MunI CAATTG 2 cut(s) 322, 1081
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 1 cut(s) 978
MwoI GCNNNNNNNGC 5 cut(s) 41, 245, 794, 872, 1240
NdeI CATATG 1 cut(s) 702
NdeII GATC 4 cut(s) 226, 480, 715, 1149
NlaIII CATG 6 cut(s) 157, 218, 243, 262, 503, 537
NlaIV GGNNCC 3 cut(s) 658, 675, 942
NmuCI GTSAC 1 cut(s) 502
NspI RCATGY 2 cut(s) 157, 262
PaeI GCATGC 1 cut(s) 157
PciI ACATGT 1 cut(s) 258
PciSI GCTCTTC 1 cut(s) 1233
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 4 cut(s) 425, 443, 473, 1059
PflMI CCANNNNNTGG 1 cut(s) 683
PkrI GCNGC 3 cut(s) 196, 402, 1126
PleI GAGTC 3 cut(s) 173, 284, 1090
PpsI GAGTC 3 cut(s) 173, 284, 1090
PscI ACATGT 1 cut(s) 258
PsiI TTATAA 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 976
PspFI CCCAGC 1 cut(s) 107
PspGI CCWGG 1 cut(s) 976
PspN4I GGNNCC 3 cut(s) 658, 675, 942
PspPI GGNCC 4 cut(s) 135, 349, 556, 674
PstI CTGCAG 1 cut(s) 226
PsuI RGATCY 3 cut(s) 480, 715, 1149
PvuII CAGCTG 2 cut(s) 107, 403
RsaI GTAC 1 cut(s) 591
RsaNI GTAC 1 cut(s) 590
SapI GCTCTTC 1 cut(s) 1233
SaqAI TTAA 7 cut(s) 126, 464, 618, 764, 816, 1158, 1250
SatI GCNGC 3 cut(s) 195, 401, 1125
Sau3AI GATC 4 cut(s) 226, 480, 715, 1149
Sau96I GGNCC 4 cut(s) 135, 349, 556, 674
ScaI AGTACT 1 cut(s) 591
SchI GAGTC 3 cut(s) 174, 285, 1090
ScrFI CCNGG 1 cut(s) 978
SduI GDGCHC 2 cut(s) 777, 1245
SfaNI GCATC 5 cut(s) 44, 185, 327, 775, 1136
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 2 cut(s) 135, 556
SmlI CTYRAG 1 cut(s) 1249
SmoI CTYRAG 1 cut(s) 1249
SpeI ACTAGT 1 cut(s) 455
SphI GCATGC 1 cut(s) 157
Sse9I AATT 7 cut(s) 127, 322, 548, 750, 801, 949, 1081
SsiI CCGC 2 cut(s) 300, 386
SspI AATATT 1 cut(s) 852
SspMI CTAG 4 cut(s) 456, 755, 842, 1261
StyD4I CCNGG 1 cut(s) 976
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 1 cut(s) 508
TaqI TCGA 1 cut(s) 428
TasI AATT 7 cut(s) 127, 322, 548, 750, 801, 949, 1081
TatI WGTACW 1 cut(s) 589
TfiI GAWTC 4 cut(s) 425, 443, 473, 1059
Tru1I TTAA 7 cut(s) 126, 464, 618, 764, 816, 1158, 1250
Tru9I TTAA 7 cut(s) 126, 464, 618, 764, 816, 1158, 1250
TscAI CASTG 4 cut(s) 795, 868, 915, 971
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 3 cut(s) 194, 400, 1124
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 5 cut(s) 111, 172, 193, 1132, 1203
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 4 cut(s) 795, 868, 915, 971
Van91I CCANNNNNTGG 1 cut(s) 683
Vha464I CTTAAG 1 cut(s) 1249
VpaK11BI GGWCC 2 cut(s) 135, 556
XapI RAATTY 2 cut(s) 548, 750
XceI RCATGY 2 cut(s) 157, 262
XmiI GTMKAC 2 cut(s) 265, 1116
XspI CTAG 4 cut(s) 456, 755, 842, 1261
ZrmI AGTACT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.