MD16G1281900.v1.1

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001699
Physical Location & Seq
Forward (+)
38428052 .. 38434511
6460 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1281900.v1.1.491

Sequence Viewer

Length: 1590 bp
ATGGCGGAATCACAACAACGAGAGAAGCAACAGGAGCAGCACCACCATCAACCGCCTCCTCCAACCCCTACTCCGCCGTCCGATCCTCCCAACCCAGTTATGTCGGAGAGCCCCATCTCAGACTCACCTCGATATCCATCATGGTTCACCCCCAAAAGGTTGCTCGTGATGTTCTGTGTGATTAACTTGATTAATTATGTCGATCGAGGAGCAATAGCCAGCAATGGTGTCAACGGTAGCATTGGGATTTGTGATCCTAGCGGCATTTGCACCGCGGGTACCGGAATTCAGGGAGATTTTAAATTGAGCAATTTTGAAGACGGCTCTCTGTCATCTGCATTTATGGTTGGGCTTCTTGTGGCCTCACCCATCTTTGCATCGTTGGCAAAGAGTCACAATCCCTTTAGGCTGATTGGAGTTGGATTATCTATTTGGACATTTGCCACAGCCGGGTGTGGTGGTTCAATTAACTTTTGGACCATTGCAATATGTCGCATGCTGGTTGGAGTTGGTGAGGCATCTTTCATTAGTCTTGCAGCACCATTCATCGATGACCATGCTCCTGCAACTCAGAAAACAGCATGGCTTGCTATGTTTTACATGTGTATACCAACTGGAATTGCCTTGGGCTATGTATACGGCGGATTTGTGGGATCCAGTTTCAGTTGGCGTTATGCATTCTGGGGAGAGGCATTAAGTATGCTTCCATTTTGTGTATTGGCTTTTGTGATGAAACCATTGGAGTTGAAAGGTCTTGCTCCTGCTGACACAAGAAAAACTTTGGAACCTATTGAAGCAACTAGTCCTTTAATTGAAGATTCAGATGTCACAGAAAAAGCTTCTTGGCCAAGCCCTGCTAAAAAGTCTTTGAGCCAACTCTCAAGATTCTCAAGAGATATGAAAGCGCTTTTGCTCGATAAAGTTTATGTTGCTAATGTCTTAGGTTATGTGTCATATAACTTTGTCATTGGAGCCTACTCGTACTGGGGACCAAAGGCTGGTTATGACATTTATCATATGAATAAGCCAGATTTGTGGTTCGGAGCTATTACAATTGTTTGTGGAATTCTGGGGACGTTAGCTGGAGGGCTCATCCTTGATGCAGTGACTGCCACAATTTATAACGCTTTTAAGCTTCTCTCTGTTGCAACATTTCTAGGCGCAATATTTTGCTTCAGTGCCTTCTGTGTAAGGAACTTGTATGGTTTTGTAGTTCTCTTCGCATTGGGGGAGCTCCTTGTGTTTGCAACCCAGGCTCCAGTTAATTATGTGTCTCTGCGGTGTGTGACACCAAGTCTTAGGCCAATGGCTATGGCTGTCTCAACTGTTTCAATCCACATCTTTGGTGATGTTCCTTCCTCGCCTCTTGCTGGAATACTCCAGGACCATGTTAAAAATTGGAGGATAACAACTCTTGCTGTGACATCTGTTCTGTTTCTTGCGTCTGGAATATGGTTCCTAGGGATCTTCGTTAGCAACAAGGGTAGTTCTGACGAAGATGGTAAGGAAGAAGTCTCCACGGTTGAGACAGTCAAAAAACCACTTGAAGAGAACAGAACTGAGGGAGCAAAAGACCCTGTTGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

530

Amino Acids

57.16

Weight (kDa)

5.94

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 95 - 444 8.8e-31 Major Facilitator Superfamily
MFS_4 PF06779 102 - 261 4.6e-06 Uncharacterised MFS-type transporter YbfB
OATP PF03137 159 - 396 3.3e-18 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 396 - 463 7.2e-08 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1122
Acc65I GGTACC 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 278
AccB7I CCANNNNNTGG 1 cut(s) 998
AccI GTMKAC 2 cut(s) 607, 636
AccII CGCG 1 cut(s) 275
AciI CCGC 8 cut(s) 5, 53, 74, 261, 273, 275, 642, 1279
AclWI GGATC 5 cut(s) 77, 248, 648, 661, 1472
AcoI YGGCCR 1 cut(s) 845
AcsI RAATTY 2 cut(s) 285, 1065
AcuI CTGAAG 1 cut(s) 1159
AfaI GTAC 2 cut(s) 280, 983
AfeI AGCGCT 1 cut(s) 906
AfiI CCNNNNNNNGG 4 cut(s) 156, 450, 998, 1370
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 8 cut(s) 317, 465, 748, 794, 815, 1332, 1547, 1583
AhdI GACNNNNNGTC 1 cut(s) 1293
AhlI ACTAGT 1 cut(s) 800
AjnI CCWGG 2 cut(s) 1251, 1380
AluBI AGCT 5 cut(s) 839, 1046, 1082, 1135, 1234
AluI AGCT 5 cut(s) 839, 1046, 1082, 1135, 1234
Alw21I GWGCWC 1 cut(s) 1236
Alw26I GTCTC 4 cut(s) 1278, 1324, 1519, 1520
AlwI GGATC 5 cut(s) 77, 248, 648, 661, 1472
AlwNI CAGNNNCTG 1 cut(s) 1109
Aor51HI AGCGCT 1 cut(s) 906
AoxI GGCC 3 cut(s) 360, 845, 1301
ApeKI GCWGC 2 cut(s) 37, 536
ApoI RAATTY 2 cut(s) 285, 1065
AseI ATTAAT 1 cut(s) 192
Asp718I GGTACC 1 cut(s) 278
AspA2I CCTAGG 1 cut(s) 1459
AspLEI GCGC 2 cut(s) 907, 1163
AspS9I GGNCC 3 cut(s) 477, 989, 1384
AsuC2I CCSGG 1 cut(s) 451
AsuHPI GGTGA 5 cut(s) 117, 139, 357, 524, 1358
AvaII GGWCC 3 cut(s) 477, 989, 1384
AvrII CCTAGG 1 cut(s) 1459
BalI TGGCCA 1 cut(s) 847
BamHI GGATCC 1 cut(s) 653
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 3 cut(s) 113, 1092, 1236
BauI CACGAG 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 1236
BbvI GCAGC 2 cut(s) 49, 548
BccI CCATC 5 cut(s) 54, 122, 145, 377, 1493
BceAI ACGGC 3 cut(s) 61, 337, 655
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 2 cut(s) 1253, 1382
BcnI CCSGG 1 cut(s) 451
BcoDI GTCTC 4 cut(s) 1278, 1324, 1519, 1520
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 4 cut(s) 258, 801, 1157, 1460
BfoI RGCGCY 1 cut(s) 908
BisI GCNGC 3 cut(s) 38, 262, 537
BlnI CCTAGG 1 cut(s) 1459
BlsI GCNGC 3 cut(s) 39, 263, 538
Bme1390I CCNGG 3 cut(s) 451, 1253, 1382
Bme18I GGWCC 3 cut(s) 477, 989, 1384
BmeRI GACNNNNNGTC 1 cut(s) 1293
BmgT120I GGNCC 3 cut(s) 477, 989, 1384
BmiI GGNNCC 7 cut(s) 280, 655, 786, 973, 990, 1257, 1457
BmrFI CCNGG 3 cut(s) 451, 1253, 1382
BmrI ACTGGG 2 cut(s) 89, 994
BmsI GCATC 3 cut(s) 386, 527, 1090
BmuI ACTGGG 2 cut(s) 89, 994
BpiI GAAGAC 1 cut(s) 324
BpmI CTGGAG 3 cut(s) 1104, 1242, 1364
BpuEI CTTGAG 2 cut(s) 865, 874
BpuMI CCSGG 1 cut(s) 451
Bsa29I ATCGAT 1 cut(s) 549
BsaBI GATNNNNATC 1 cut(s) 136
BsaJI CCNNGG 5 cut(s) 273, 624, 1251, 1459, 1518
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 4 cut(s) 55, 85, 1240, 1270
Bsc4I CCNNNNNNNGG 4 cut(s) 156, 450, 998, 1370
Bse1I ACTGG 5 cut(s) 95, 619, 657, 989, 1259
Bse3DI GCAATG 2 cut(s) 229, 480
Bse8I GATNNNNATC 1 cut(s) 136
BseBI CCWGG 2 cut(s) 1253, 1382
BseCI ATCGAT 1 cut(s) 549
BseDI CCNNGG 5 cut(s) 273, 624, 1251, 1459, 1518
BseGI GGATG 1 cut(s) 1092
BseJI GATNNNNATC 1 cut(s) 136
BseLI CCNNNNNNNGG 4 cut(s) 156, 450, 998, 1370
BseMI GCAATG 2 cut(s) 229, 480
BseMII CTCAG 3 cut(s) 132, 584, 1551
BseNI ACTGG 5 cut(s) 95, 619, 657, 989, 1259
BseRI GAGGAG 2 cut(s) 48, 222
BseXI GCAGC 2 cut(s) 49, 548
Bsh1236I CGCG 1 cut(s) 275
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 3 cut(s) 362, 847, 1303
BshNI GGYRCC 1 cut(s) 278
BshVI ATCGAT 1 cut(s) 549
BsiEI CGRYCG 1 cut(s) 205
BsiHKAI GWGCWC 1 cut(s) 1236
BsiSI CCGG 2 cut(s) 282, 450
BslFI GGGAC 2 cut(s) 1002, 1087
BslI CCNNNNNNNGG 4 cut(s) 156, 450, 998, 1370
BsmAI GTCTC 4 cut(s) 1278, 1324, 1519, 1520
BsmFI GGGAC 2 cut(s) 1002, 1087
BsmI GAATGC 1 cut(s) 677
BsnI GGCC 3 cut(s) 362, 847, 1303
Bsp1286I GDGCHC 3 cut(s) 113, 1092, 1236
Bsp143I GATC 5 cut(s) 82, 202, 253, 653, 1464
BspACI CCGC 8 cut(s) 5, 53, 74, 261, 273, 275, 642, 1279
BspANI GGCC 3 cut(s) 362, 847, 1303
BspCNI CTCAG 3 cut(s) 131, 583, 1552
BspDI ATCGAT 1 cut(s) 549
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 7 cut(s) 280, 655, 786, 973, 990, 1257, 1457
BspPI GGATC 5 cut(s) 77, 248, 648, 661, 1472
BspT107I GGYRCC 1 cut(s) 278
BsrDI GCAATG 2 cut(s) 229, 480
BsrI ACTGG 5 cut(s) 95, 619, 657, 989, 1259
BssECI CCNNGG 5 cut(s) 273, 624, 1251, 1459, 1518
BssMI GATC 5 cut(s) 82, 202, 253, 653, 1464
BssNAI GTATAC 2 cut(s) 608, 637
BssSI CACGAG 1 cut(s) 164
BssT1I CCWWGG 2 cut(s) 624, 1459
Bst1107I GTATAC 2 cut(s) 608, 637
Bst2BI CACGAG 1 cut(s) 164
Bst2UI CCWGG 2 cut(s) 1253, 1382
Bst4CI ACNGT 4 cut(s) 236, 1327, 1522, 1531
Bst6I CTCTTC 2 cut(s) 1223, 1542
BstAPI GCANNNNNTGC 2 cut(s) 587, 1109
BstC8I GCNNGC 3 cut(s) 220, 497, 588
BstDEI CTNAG 5 cut(s) 118, 570, 940, 1298, 1560
BstDSI CCRYGG 2 cut(s) 273, 1518
BstF5I GGATG 1 cut(s) 1092
BstFNI CGCG 1 cut(s) 275
BstH2I RGCGCY 1 cut(s) 908
BstHHI GCGC 2 cut(s) 907, 1163
BstKTI GATC 5 cut(s) 85, 205, 256, 656, 1467
BstMAI GTCTC 4 cut(s) 1278, 1324, 1519, 1520
BstMBI GATC 5 cut(s) 82, 202, 253, 653, 1464
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 6 cut(s) 34, 267, 383, 587, 1109, 1253
BstNI CCWGG 2 cut(s) 1253, 1382
BstNSI RCATGY 2 cut(s) 499, 604
BstSCI CCNGG 3 cut(s) 449, 1251, 1380
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 2 cut(s) 49, 548
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 2 cut(s) 653, 1464
BstXI CCANNNNNNTGG 2 cut(s) 1035, 1343
BstYI RGATCY 2 cut(s) 653, 1464
BstZ17I GTATAC 2 cut(s) 608, 637
Bsu15I ATCGAT 1 cut(s) 549
BsuRI GGCC 3 cut(s) 362, 847, 1303
BsuTUI ATCGAT 1 cut(s) 549
BtgI CCRYGG 2 cut(s) 273, 1518
BtsCI GGATG 1 cut(s) 1092
BtsI GCAGTG 1 cut(s) 1110
BtsIMutI CAGTG 2 cut(s) 1110, 1183
Cac8I GCNNGC 3 cut(s) 220, 497, 588
CaiI CAGNNNCTG 1 cut(s) 1109
CfoI GCGC 2 cut(s) 907, 1163
Cfr13I GGNCC 3 cut(s) 477, 989, 1384
Cfr42I CCGCGG 1 cut(s) 276
ClaI ATCGAT 1 cut(s) 549
CseI GACGC 1 cut(s) 1431
Csp6I GTAC 2 cut(s) 279, 982
CviAII CATG 6 cut(s) 141, 496, 557, 582, 601, 1388
CviQI GTAC 2 cut(s) 279, 982
DdeI CTNAG 5 cut(s) 118, 570, 940, 1298, 1560
DpnI GATC 5 cut(s) 84, 204, 255, 655, 1466
DpnII GATC 5 cut(s) 82, 202, 253, 653, 1464
DraI TTTAAA 1 cut(s) 301
DriI GACNNNNNGTC 1 cut(s) 1293
EaeI YGGCCR 1 cut(s) 845
Eam1104I CTCTTC 2 cut(s) 1223, 1542
Eam1105I GACNNNNNGTC 1 cut(s) 1293
EarI CTCTTC 2 cut(s) 1223, 1542
EciI GGCGGA 3 cut(s) 20, 63, 657
Ecl136II GAGCTC 1 cut(s) 1234
Eco130I CCWWGG 2 cut(s) 624, 1459
Eco24I GRGCYC 3 cut(s) 113, 1092, 1236
Eco32I GATATC 1 cut(s) 134
Eco47I GGWCC 3 cut(s) 477, 989, 1384
Eco47III AGCGCT 1 cut(s) 906
Eco53kI GAGCTC 1 cut(s) 1234
Eco57I CTGAAG 1 cut(s) 1159
EcoICRI GAGCTC 1 cut(s) 1234
EcoRI GAATTC 2 cut(s) 285, 1065
EcoRII CCWGG 2 cut(s) 1251, 1380
EcoRV GATATC 1 cut(s) 134
EcoT14I CCWWGG 2 cut(s) 624, 1459
EcoT22I ATGCAT 1 cut(s) 679
EcoT38I GRGCYC 3 cut(s) 113, 1092, 1236
ErhI CCWWGG 2 cut(s) 624, 1459
FaeI CATG 6 cut(s) 144, 499, 560, 585, 604, 1391
FalI AAGNNNNNCTT 2 cut(s) 763, 795
FaqI GGGAC 2 cut(s) 1002, 1087
FatI CATG 6 cut(s) 140, 495, 556, 581, 600, 1387
FauI CCCGC 1 cut(s) 268
FauNDI CATATG 1 cut(s) 1017
FblI GTMKAC 2 cut(s) 607, 636
Fnu4HI GCNGC 3 cut(s) 38, 262, 537
FokI GGATG 1 cut(s) 1079
FriOI GRGCYC 3 cut(s) 113, 1092, 1236
Fsp4HI GCNGC 3 cut(s) 38, 262, 537
FspBI CTAG 4 cut(s) 258, 801, 1157, 1460
GlaI GCGC 2 cut(s) 906, 1162
GluI GCNGC 3 cut(s) 38, 262, 537
GsuI CTGGAG 3 cut(s) 1104, 1242, 1364
HaeII RGCGCY 1 cut(s) 908
HaeIII GGCC 3 cut(s) 362, 847, 1303
HapII CCGG 2 cut(s) 282, 450
HgaI GACGC 1 cut(s) 1431
HhaI GCGC 2 cut(s) 907, 1163
Hin1II CATG 6 cut(s) 144, 499, 560, 585, 604, 1391
Hin6I GCGC 2 cut(s) 905, 1161
HinP1I GCGC 2 cut(s) 905, 1161
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HindIII AAGCTT 2 cut(s) 837, 1133
HinfI GANTC 5 cut(s) 8, 122, 391, 818, 885
HpaII CCGG 2 cut(s) 282, 450
HphI GGTGA 5 cut(s) 117, 139, 357, 524, 1358
Hpy166II GTNNAC 4 cut(s) 147, 232, 608, 637
Hpy188I TCNGA 7 cut(s) 82, 106, 121, 573, 823, 1043, 1492
Hpy188III TCNNGA 4 cut(s) 166, 882, 891, 1446
Hpy8I GTNNAC 4 cut(s) 147, 232, 608, 637
HpyAV CCTTC 2 cut(s) 1192, 1365
HpyCH4III ACNGT 4 cut(s) 236, 1327, 1522, 1531
HpyCH4IV ACGT 1 cut(s) 1076
HpyF10VI GCNNNNNNNGC 6 cut(s) 34, 267, 383, 587, 1109, 1253
HpyF3I CTNAG 5 cut(s) 118, 570, 940, 1298, 1560
HpySE526I ACGT 1 cut(s) 1076
Hsp92II CATG 6 cut(s) 144, 499, 560, 585, 604, 1391
HspAI GCGC 2 cut(s) 905, 1161
KpnI GGTACC 1 cut(s) 282
KspI CCGCGG 1 cut(s) 276
Kzo9I GATC 5 cut(s) 82, 202, 253, 653, 1464
Lsp1109I GCAGC 2 cut(s) 49, 548
LweI GCATC 3 cut(s) 386, 527, 1090
MaeI CTAG 4 cut(s) 258, 801, 1157, 1460
MaeII ACGT 1 cut(s) 1076
MaeIII GTNAC 5 cut(s) 392, 826, 1105, 1285, 1420
MalI GATC 5 cut(s) 84, 204, 255, 655, 1466
MboI GATC 5 cut(s) 82, 202, 253, 653, 1464
MboII GAAGA 7 cut(s) 329, 827, 1210, 1459, 1508, 1520, 1559
MfeI CAATTG 1 cut(s) 1053
MflI RGATCY 2 cut(s) 653, 1464
MhlI GDGCHC 3 cut(s) 113, 1092, 1236
MlsI TGGCCA 1 cut(s) 847
MluNI TGGCCA 1 cut(s) 847
MlyI GAGTC 2 cut(s) 116, 400
MmeI TCCRAC 4 cut(s) 84, 86, 400, 484
Mox20I TGGCCA 1 cut(s) 847
Mph1103I ATGCAT 1 cut(s) 679
MscI TGGCCA 1 cut(s) 847
MseI TTAA 9 cut(s) 183, 192, 300, 468, 695, 809, 1131, 1263, 1392
Msp20I TGGCCA 1 cut(s) 847
MspA1I CMGCKG 1 cut(s) 275
MspI CCGG 2 cut(s) 282, 450
MspR9I CCNGG 3 cut(s) 451, 1253, 1382
MunI CAATTG 1 cut(s) 1053
Mva1269I GAATGC 1 cut(s) 677
MvaI CCWGG 2 cut(s) 1253, 1382
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 6 cut(s) 34, 267, 383, 587, 1109, 1253
NciI CCSGG 1 cut(s) 451
NdeI CATATG 1 cut(s) 1017
NdeII GATC 5 cut(s) 82, 202, 253, 653, 1464
NlaIII CATG 6 cut(s) 144, 499, 560, 585, 604, 1391
NlaIV GGNNCC 7 cut(s) 280, 655, 786, 973, 990, 1257, 1457
NmuCI GTSAC 5 cut(s) 392, 826, 1105, 1285, 1420
NsiI ATGCAT 1 cut(s) 679
NspI RCATGY 2 cut(s) 499, 604
PaeI GCATGC 1 cut(s) 499
PciI ACATGT 1 cut(s) 600
PctI GAATGC 1 cut(s) 677
PfeI GAWTC 3 cut(s) 8, 818, 885
PflMI CCANNNNNTGG 1 cut(s) 998
PfoI TCCNGGA 1 cut(s) 1380
PkrI GCNGC 3 cut(s) 39, 263, 538
Ple19I CGATCG 1 cut(s) 205
PleI GAGTC 2 cut(s) 116, 399
PpsI GAGTC 2 cut(s) 116, 399
PscI ACATGT 1 cut(s) 600
PshBI ATTAAT 1 cut(s) 192
PsiI TTATAA 1 cut(s) 1122
Psp124BI GAGCTC 1 cut(s) 1236
Psp6I CCWGG 2 cut(s) 1251, 1380
PspGI CCWGG 2 cut(s) 1251, 1380
PspN4I GGNNCC 7 cut(s) 280, 655, 786, 973, 990, 1257, 1457
PspPI GGNCC 3 cut(s) 477, 989, 1384
PstNI CAGNNNCTG 1 cut(s) 1109
PsuI RGATCY 2 cut(s) 653, 1464
PvuI CGATCG 1 cut(s) 205
RsaI GTAC 2 cut(s) 280, 983
RsaNI GTAC 2 cut(s) 279, 982
SacI GAGCTC 1 cut(s) 1236
SacII CCGCGG 1 cut(s) 276
SaqAI TTAA 9 cut(s) 183, 192, 300, 468, 695, 809, 1131, 1263, 1392
SatI GCNGC 3 cut(s) 38, 262, 537
Sau3AI GATC 5 cut(s) 82, 202, 253, 653, 1464
Sau96I GGNCC 3 cut(s) 477, 989, 1384
SchI GAGTC 2 cut(s) 116, 400
ScrFI CCNGG 3 cut(s) 451, 1253, 1382
SduI GDGCHC 3 cut(s) 113, 1092, 1236
SfaNI GCATC 3 cut(s) 386, 527, 1090
Sfr303I CCGCGG 1 cut(s) 276
SgrBI CCGCGG 1 cut(s) 276
SinI GGWCC 3 cut(s) 477, 989, 1384
SmlI CTYRAG 2 cut(s) 880, 889
SmoI CTYRAG 2 cut(s) 880, 889
SpeI ACTAGT 1 cut(s) 800
SphI GCATGC 1 cut(s) 499
SsiI CCGC 8 cut(s) 5, 53, 74, 261, 273, 275, 642, 1279
SspI AATATT 1 cut(s) 1167
SspMI CTAG 4 cut(s) 258, 801, 1157, 1460
SstI GAGCTC 1 cut(s) 1236
StyD4I CCNGG 3 cut(s) 449, 1251, 1380
StyI CCWWGG 2 cut(s) 624, 1459
TaaI ACNGT 4 cut(s) 236, 1327, 1522, 1531
TaiI ACGT 1 cut(s) 1079
TaqI TCGA 5 cut(s) 130, 201, 205, 549, 915
TauI GCSGC 1 cut(s) 264
TfiI GAWTC 3 cut(s) 8, 818, 885
Tru1I TTAA 9 cut(s) 183, 192, 300, 468, 695, 809, 1131, 1263, 1392
Tru9I TTAA 9 cut(s) 183, 192, 300, 468, 695, 809, 1131, 1263, 1392
TscAI CASTG 2 cut(s) 1110, 1183
TseFI GTSAC 5 cut(s) 392, 826, 1105, 1285, 1420
TseI GCWGC 2 cut(s) 37, 536
Tsp45I GTSAC 5 cut(s) 392, 826, 1105, 1285, 1420
TspDTI ATGAA 5 cut(s) 514, 535, 746, 914, 1034
TspRI CASTG 2 cut(s) 1110, 1183
Van91I CCANNNNNTGG 1 cut(s) 998
VpaK11BI GGWCC 3 cut(s) 477, 989, 1384
VspI ATTAAT 1 cut(s) 192
XapI RAATTY 2 cut(s) 285, 1065
XceI RCATGY 2 cut(s) 499, 604
XmaJI CCTAGG 1 cut(s) 1459
XmiI GTMKAC 2 cut(s) 607, 636
XspI CTAG 4 cut(s) 258, 801, 1157, 1460
Zsp2I ATGCAT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.