Belongs to the peptidase S8 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 158, 1625 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1418 |
| AccBSI | CCGCTC | 1 cut(s) | 1004 |
| AccII | CGCG | 5 cut(s) | 218, 662, 713, 737, 1361 |
| AciI | CCGC | 9 cut(s) | 59, 96, 218, 690, 735, 939, 1004, 1365, 1560 |
| AclI | AACGTT | 1 cut(s) | 1040 |
| AclWI | GGATC | 2 cut(s) | 872, 1209 |
| AcoI | YGGCCR | 4 cut(s) | 353, 580, 1511, 2184 |
| AcsI | RAATTY | 4 cut(s) | 334, 405, 479, 1280 |
| AcuI | CTGAAG | 1 cut(s) | 135 |
| AfaI | GTAC | 3 cut(s) | 349, 599, 1732 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 123, 351, 408, 1418 |
| AflIII | ACRYGT | 1 cut(s) | 660 |
| AgeI | ACCGGT | 1 cut(s) | 289 |
| AjiI | CACGTC | 1 cut(s) | 1917 |
| AjnI | CCWGG | 3 cut(s) | 1081, 1486, 1750 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 561, 593 |
| Alw21I | GWGCWC | 2 cut(s) | 240, 1546 |
| Alw26I | GTCTC | 3 cut(s) | 56, 274, 364 |
| AlwI | GGATC | 2 cut(s) | 872, 1209 |
| Ama87I | CYCGRG | 2 cut(s) | 260, 1539 |
| AoxI | GGCC | 7 cut(s) | 353, 410, 580, 950, 1511, 1753, 2184 |
| ApeKI | GCWGC | 9 cut(s) | 629, 638, 773, 902, 1131, 1226, 1229, 1592, 1616 |
| ApoI | RAATTY | 4 cut(s) | 334, 405, 479, 1280 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1669, 1701 |
| AsiGI | ACCGGT | 1 cut(s) | 289 |
| AspLEI | GCGC | 5 cut(s) | 218, 715, 982, 1411, 1621 |
| AspS9I | GGNCC | 3 cut(s) | 411, 950, 1439 |
| AsuC2I | CCSGG | 3 cut(s) | 261, 262, 1579 |
| AsuHPI | GGTGA | 5 cut(s) | 217, 268, 585, 1522, 2143 |
| AvaI | CYCGRG | 2 cut(s) | 260, 1539 |
| AvaII | GGWCC | 1 cut(s) | 1439 |
| AxyI | CCTNAGG | 1 cut(s) | 694 |
| BalI | TGGCCA | 2 cut(s) | 582, 2186 |
| BanII | GRGCYC | 1 cut(s) | 240 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2057, 2089 |
| BauI | CACGAG | 3 cut(s) | 1802, 1840, 2188 |
| Bbv12I | GWGCWC | 2 cut(s) | 240, 1546 |
| BbvI | GCAGC | 9 cut(s) | 641, 650, 785, 914, 1143, 1213, 1241, 1579, 1603 |
| BccI | CCATC | 7 cut(s) | 431, 791, 851, 1078, 1644, 1660, 1882 |
| BceAI | ACGGC | 6 cut(s) | 72, 631, 967, 1498, 1694, 1698 |
| BciT130I | CCWGG | 3 cut(s) | 1083, 1488, 1752 |
| BclI | TGATCA | 3 cut(s) | 781, 1827, 1921 |
| BcnI | CCSGG | 3 cut(s) | 261, 262, 1579 |
| BcoDI | GTCTC | 3 cut(s) | 56, 274, 364 |
| BfaI | CTAG | 4 cut(s) | 719, 824, 869, 1481 |
| BfmI | CTRYAG | 1 cut(s) | 1227 |
| BfoI | RGCGCY | 2 cut(s) | 983, 1412 |
| BglII | AGATCT | 1 cut(s) | 1938 |
| BlpI | GCTNAGC | 1 cut(s) | 2069 |
| Bme1390I | CCNGG | 6 cut(s) | 261, 262, 1083, 1488, 1579, 1752 |
| Bme18I | GGWCC | 1 cut(s) | 1439 |
| BmeT110I | CYCGRG | 2 cut(s) | 260, 1539 |
| BmgBI | CACGTC | 1 cut(s) | 1917 |
| BmgT120I | GGNCC | 3 cut(s) | 411, 950, 1439 |
| BmiI | GGNNCC | 4 cut(s) | 453, 688, 1033, 1440 |
| BmrFI | CCNGG | 6 cut(s) | 261, 262, 1083, 1488, 1579, 1752 |
| BmsI | GCATC | 3 cut(s) | 724, 902, 1349 |
| BpmI | CTGGAG | 4 cut(s) | 982, 1104, 1394, 1629 |
| Bpu10I | CCTNAGC | 1 cut(s) | 522 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2069 |
| BpuEI | CTTGAG | 2 cut(s) | 1280, 1417 |
| BpuMI | CCSGG | 3 cut(s) | 261, 262, 1579 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1832 |
| BsaI | GGTCTC | 2 cut(s) | 56, 364 |
| BsaJI | CCNNGG | 5 cut(s) | 260, 682, 983, 1487, 1750 |
| BsaWI | WCCGGW | 5 cut(s) | 289, 344, 401, 1220, 1532 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 298, 328, 792, 822 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 123, 351, 408, 1418 |
| Bse118I | RCCGGY | 2 cut(s) | 289, 355 |
| Bse1I | ACTGG | 4 cut(s) | 965, 1109, 1285, 1292 |
| Bse21I | CCTNAGG | 1 cut(s) | 694 |
| Bse3DI | GCAATG | 2 cut(s) | 633, 1902 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1832 |
| BseBI | CCWGG | 3 cut(s) | 1083, 1488, 1752 |
| BseDI | CCNNGG | 5 cut(s) | 260, 682, 983, 1487, 1750 |
| BseGI | GGATG | 5 cut(s) | 12, 78, 341, 847, 1636 |
| BseJI | GATNNNNATC | 1 cut(s) | 1832 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 123, 351, 408, 1418 |
| BseMI | GCAATG | 2 cut(s) | 633, 1902 |
| BseMII | CTCAG | 3 cut(s) | 225, 708, 2148 |
| BseNI | ACTGG | 4 cut(s) | 965, 1109, 1285, 1292 |
| BseRI | GAGGAG | 1 cut(s) | 1277 |
| BseXI | GCAGC | 9 cut(s) | 641, 650, 785, 914, 1143, 1213, 1241, 1579, 1603 |
| BseYI | CCCAGC | 2 cut(s) | 422, 641 |
| BsgI | GTGCAG | 1 cut(s) | 1578 |
| Bsh1236I | CGCG | 5 cut(s) | 218, 662, 713, 737, 1361 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1365 |
| BshFI | GGCC | 7 cut(s) | 355, 412, 582, 952, 1513, 1755, 2186 |
| BshTI | ACCGGT | 1 cut(s) | 289 |
| BsiEI | CGRYCG | 1 cut(s) | 1365 |
| BsiHKAI | GWGCWC | 2 cut(s) | 240, 1546 |
| BsiHKCI | CYCGRG | 2 cut(s) | 260, 1539 |
| BsiSI | CCGG | 9 cut(s) | 261, 290, 345, 356, 402, 936, 1221, 1533, 1578 |
| BsiWI | CGTACG | 1 cut(s) | 597 |
| BslFI | GGGAC | 5 cut(s) | 405, 1411, 1504, 1594, 1917 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 123, 351, 408, 1418 |
| BsmAI | GTCTC | 3 cut(s) | 56, 274, 364 |
| BsmFI | GGGAC | 5 cut(s) | 405, 1411, 1504, 1594, 1917 |
| BsmI | GAATGC | 1 cut(s) | 1298 |
| BsnI | GGCC | 7 cut(s) | 355, 412, 582, 952, 1513, 1755, 2186 |
| Bso31I | GGTCTC | 2 cut(s) | 56, 364 |
| BsoBI | CYCGRG | 2 cut(s) | 260, 1539 |
| Bsp1286I | GDGCHC | 2 cut(s) | 240, 1546 |
| Bsp143I | GATC | 8 cut(s) | 118, 781, 877, 1214, 1827, 1844, 1921, 1938 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2069 |
| BspACI | CCGC | 9 cut(s) | 59, 96, 218, 690, 735, 939, 1004, 1365, 1560 |
| BspANI | GGCC | 7 cut(s) | 355, 412, 582, 952, 1513, 1755, 2186 |
| BspCNI | CTCAG | 3 cut(s) | 226, 707, 2149 |
| BspFNI | CGCG | 5 cut(s) | 218, 662, 713, 737, 1361 |
| BspLI | GGNNCC | 4 cut(s) | 453, 688, 1033, 1440 |
| BspMAI | CTGCAG | 1 cut(s) | 1231 |
| BspPI | GGATC | 2 cut(s) | 872, 1209 |
| BspTNI | GGTCTC | 2 cut(s) | 56, 364 |
| BsrBI | CCGCTC | 1 cut(s) | 1004 |
| BsrDI | GCAATG | 2 cut(s) | 633, 1902 |
| BsrFI | RCCGGY | 2 cut(s) | 289, 355 |
| BsrI | ACTGG | 4 cut(s) | 965, 1109, 1285, 1292 |
| BssAI | RCCGGY | 2 cut(s) | 289, 355 |
| BssECI | CCNNGG | 5 cut(s) | 260, 682, 983, 1487, 1750 |
| BssMI | GATC | 8 cut(s) | 118, 781, 877, 1214, 1827, 1844, 1921, 1938 |
| BssSI | CACGAG | 3 cut(s) | 1802, 1840, 2188 |
| BssT1I | CCWWGG | 1 cut(s) | 682 |
| Bst2BI | CACGAG | 3 cut(s) | 1802, 1840, 2188 |
| Bst2UI | CCWGG | 3 cut(s) | 1083, 1488, 1752 |
| Bst4CI | ACNGT | 7 cut(s) | 13, 105, 597, 997, 1390, 1551, 2080 |
| Bst6I | CTCTTC | 1 cut(s) | 595 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 635, 899 |
| BstDEI | CTNAG | 5 cut(s) | 234, 522, 694, 2069, 2157 |
| BstDSI | CCRYGG | 1 cut(s) | 983 |
| BstF5I | GGATG | 5 cut(s) | 12, 78, 341, 847, 1636 |
| BstFNI | CGCG | 5 cut(s) | 218, 662, 713, 737, 1361 |
| BstH2I | RGCGCY | 2 cut(s) | 983, 1412 |
| BstHHI | GCGC | 5 cut(s) | 218, 715, 982, 1411, 1621 |
| BstKTI | GATC | 8 cut(s) | 121, 784, 880, 1217, 1830, 1847, 1924, 1941 |
| BstMAI | GTCTC | 3 cut(s) | 56, 274, 364 |
| BstMBI | GATC | 8 cut(s) | 118, 781, 877, 1214, 1827, 1844, 1921, 1938 |
| BstMCI | CGRYCG | 1 cut(s) | 1365 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 635, 638, 710, 719, 899, 1428, 1613, 1627, 1715 |
| BstNI | CCWGG | 3 cut(s) | 1083, 1488, 1752 |
| BstNSI | RCATGY | 2 cut(s) | 477, 1727 |
| BstSCI | CCNGG | 6 cut(s) | 259, 260, 1081, 1486, 1577, 1750 |
| BstSFI | CTRYAG | 1 cut(s) | 1227 |
| BstUI | CGCG | 5 cut(s) | 218, 662, 713, 737, 1361 |
| BstV1I | GCAGC | 9 cut(s) | 641, 650, 785, 914, 1143, 1213, 1241, 1579, 1603 |
| BstX2I | RGATCY | 2 cut(s) | 1214, 1938 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 960 |
| BstYI | RGATCY | 2 cut(s) | 1214, 1938 |
| Bsu36I | CCTNAGG | 1 cut(s) | 694 |
| BsuRI | GGCC | 7 cut(s) | 355, 412, 582, 952, 1513, 1755, 2186 |
| BtgI | CCRYGG | 1 cut(s) | 983 |
| BtrI | CACGTC | 1 cut(s) | 1917 |
| BtsCI | GGATG | 5 cut(s) | 12, 78, 341, 847, 1636 |
| BtsIMutI | CAGTG | 1 cut(s) | 2076 |
| CfoI | GCGC | 5 cut(s) | 218, 715, 982, 1411, 1621 |
| Cfr10I | RCCGGY | 2 cut(s) | 289, 355 |
| Cfr13I | GGNCC | 3 cut(s) | 411, 950, 1439 |
| Cfr9I | CCCGGG | 1 cut(s) | 260 |
| CseI | GACGC | 2 cut(s) | 651, 1868 |
| Csp6I | GTAC | 3 cut(s) | 348, 598, 1731 |
| CspAI | ACCGGT | 1 cut(s) | 289 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2176, 2211 |
| CviAII | CATG | 8 cut(s) | 85, 351, 474, 623, 650, 806, 1601, 1724 |
| CviQI | GTAC | 3 cut(s) | 348, 598, 1731 |
| DdeI | CTNAG | 5 cut(s) | 234, 522, 694, 2069, 2157 |
| DpnI | GATC | 8 cut(s) | 120, 783, 879, 1216, 1829, 1846, 1923, 1940 |
| DpnII | GATC | 8 cut(s) | 118, 781, 877, 1214, 1827, 1844, 1921, 1938 |
| DraI | TTTAAA | 1 cut(s) | 333 |
| EaeI | YGGCCR | 4 cut(s) | 353, 580, 1511, 2184 |
| Eam1104I | CTCTTC | 1 cut(s) | 595 |
| EarI | CTCTTC | 1 cut(s) | 595 |
| EciI | GGCGGA | 1 cut(s) | 74 |
| Ecl136II | GAGCTC | 1 cut(s) | 238 |
| Eco130I | CCWWGG | 1 cut(s) | 682 |
| Eco24I | GRGCYC | 1 cut(s) | 240 |
| Eco31I | GGTCTC | 2 cut(s) | 56, 364 |
| Eco32I | GATATC | 1 cut(s) | 813 |
| Eco47I | GGWCC | 1 cut(s) | 1439 |
| Eco53kI | GAGCTC | 1 cut(s) | 238 |
| Eco57I | CTGAAG | 1 cut(s) | 135 |
| Eco81I | CCTNAGG | 1 cut(s) | 694 |
| Eco88I | CYCGRG | 2 cut(s) | 260, 1539 |
| EcoICRI | GAGCTC | 1 cut(s) | 238 |
| EcoRI | GAATTC | 1 cut(s) | 479 |
| EcoRII | CCWGG | 3 cut(s) | 1081, 1486, 1750 |
| EcoRV | GATATC | 1 cut(s) | 813 |
| EcoT14I | CCWWGG | 1 cut(s) | 682 |
| EcoT38I | GRGCYC | 1 cut(s) | 240 |
| ErhI | CCWWGG | 1 cut(s) | 682 |
| FaeI | CATG | 8 cut(s) | 88, 354, 477, 626, 653, 809, 1604, 1727 |
| FaqI | GGGAC | 5 cut(s) | 405, 1411, 1504, 1594, 1917 |
| FatI | CATG | 8 cut(s) | 84, 350, 473, 622, 649, 805, 1600, 1723 |
| FauI | CCCGC | 1 cut(s) | 997 |
| FbaI | TGATCA | 3 cut(s) | 781, 1827, 1921 |
| FokI | GGATG | 5 cut(s) | 19, 65, 328, 854, 1623 |
| FriOI | GRGCYC | 1 cut(s) | 240 |
| FspBI | CTAG | 4 cut(s) | 719, 824, 869, 1481 |
| GlaI | GCGC | 5 cut(s) | 217, 714, 981, 1410, 1620 |
| GsaI | CCCAGC | 2 cut(s) | 426, 645 |
| GsuI | CTGGAG | 4 cut(s) | 982, 1104, 1394, 1629 |
| HaeII | RGCGCY | 2 cut(s) | 983, 1412 |
| HaeIII | GGCC | 7 cut(s) | 355, 412, 582, 952, 1513, 1755, 2186 |
| HapII | CCGG | 9 cut(s) | 261, 290, 345, 356, 402, 936, 1221, 1533, 1578 |
| HgaI | GACGC | 2 cut(s) | 651, 1868 |
| HhaI | GCGC | 5 cut(s) | 218, 715, 982, 1411, 1621 |
| Hin1II | CATG | 8 cut(s) | 88, 354, 477, 626, 653, 809, 1604, 1727 |
| Hin6I | GCGC | 5 cut(s) | 216, 713, 980, 1409, 1619 |
| HinP1I | GCGC | 5 cut(s) | 216, 713, 980, 1409, 1619 |
| HindIII | AAGCTT | 2 cut(s) | 296, 1450 |
| HpaII | CCGG | 9 cut(s) | 261, 290, 345, 356, 402, 936, 1221, 1533, 1578 |
| HphI | GGTGA | 5 cut(s) | 217, 268, 585, 1522, 2143 |
| Hpy166II | GTNNAC | 2 cut(s) | 205, 276 |
| Hpy188I | TCNGA | 8 cut(s) | 118, 126, 235, 611, 763, 1214, 1858, 1921 |
| Hpy8I | GTNNAC | 2 cut(s) | 205, 276 |
| Hpy99I | CGWCG | 7 cut(s) | 17, 437, 950, 1519, 1862, 2024, 2027 |
| HpyAV | CCTTC | 4 cut(s) | 740, 1104, 1905, 2002 |
| HpyCH4III | ACNGT | 7 cut(s) | 13, 105, 597, 997, 1390, 1551, 2080 |
| HpyCH4IV | ACGT | 6 cut(s) | 244, 1040, 1916, 1962, 2019, 2115 |
| HpyCH4V | TGCA | 7 cut(s) | 501, 629, 638, 902, 1229, 1595, 1670 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 635, 638, 710, 719, 899, 1428, 1613, 1627, 1715 |
| HpyF3I | CTNAG | 5 cut(s) | 234, 522, 694, 2069, 2157 |
| HpySE526I | ACGT | 6 cut(s) | 244, 1040, 1916, 1962, 2019, 2115 |
| Hsp92II | CATG | 8 cut(s) | 88, 354, 477, 626, 653, 809, 1604, 1727 |
| HspAI | GCGC | 5 cut(s) | 216, 713, 980, 1409, 1619 |
| Ksp22I | TGATCA | 3 cut(s) | 781, 1827, 1921 |
| Kzo9I | GATC | 8 cut(s) | 118, 781, 877, 1214, 1827, 1844, 1921, 1938 |
| Lsp1109I | GCAGC | 9 cut(s) | 641, 650, 785, 914, 1143, 1213, 1241, 1579, 1603 |
| LweI | GCATC | 3 cut(s) | 724, 902, 1349 |
| MaeI | CTAG | 4 cut(s) | 719, 824, 869, 1481 |
| MaeII | ACGT | 6 cut(s) | 244, 1040, 1916, 1962, 2019, 2115 |
| MaeIII | GTNAC | 8 cut(s) | 52, 928, 941, 1111, 1172, 1254, 1799, 2103 |
| MalI | GATC | 8 cut(s) | 120, 783, 879, 1216, 1829, 1846, 1923, 1940 |
| MbiI | CCGCTC | 1 cut(s) | 1004 |
| MboI | GATC | 8 cut(s) | 118, 781, 877, 1214, 1827, 1844, 1921, 1938 |
| MboII | GAAGA | 5 cut(s) | 480, 483, 582, 755, 2159 |
| MfeI | CAATTG | 1 cut(s) | 1011 |
| MflI | RGATCY | 2 cut(s) | 1214, 1938 |
| MhlI | GDGCHC | 2 cut(s) | 240, 1546 |
| MlsI | TGGCCA | 2 cut(s) | 582, 2186 |
| MluI | ACGCGT | 1 cut(s) | 660 |
| MluNI | TGGCCA | 2 cut(s) | 582, 2186 |
| MlyI | GAGTC | 5 cut(s) | 810, 1141, 1620, 1657, 2101 |
| MmeI | TCCRAC | 4 cut(s) | 265, 979, 1693, 1881 |
| Mox20I | TGGCCA | 2 cut(s) | 582, 2186 |
| MscI | TGGCCA | 2 cut(s) | 582, 2186 |
| MseI | TTAA | 9 cut(s) | 249, 294, 332, 654, 972, 1241, 1574, 1745, 1944 |
| MslI | CAYNNNNRTG | 2 cut(s) | 83, 435 |
| Msp20I | TGGCCA | 2 cut(s) | 582, 2186 |
| MspA1I | CMGCKG | 2 cut(s) | 641, 1560 |
| MspI | CCGG | 9 cut(s) | 261, 290, 345, 356, 402, 936, 1221, 1533, 1578 |
| MspR9I | CCNGG | 6 cut(s) | 261, 262, 1083, 1488, 1579, 1752 |
| MunI | CAATTG | 1 cut(s) | 1011 |
| Mva1269I | GAATGC | 1 cut(s) | 1298 |
| MvaI | CCWGG | 3 cut(s) | 1083, 1488, 1752 |
| MvnI | CGCG | 5 cut(s) | 218, 662, 713, 737, 1361 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 635, 638, 710, 719, 899, 1428, 1613, 1627, 1715 |
| NciI | CCSGG | 3 cut(s) | 261, 262, 1579 |
| NdeII | GATC | 8 cut(s) | 118, 781, 877, 1214, 1827, 1844, 1921, 1938 |
| NlaIII | CATG | 8 cut(s) | 88, 354, 477, 626, 653, 809, 1604, 1727 |
| NlaIV | GGNNCC | 4 cut(s) | 453, 688, 1033, 1440 |
| NmuCI | GTSAC | 1 cut(s) | 1799 |
| NspI | RCATGY | 2 cut(s) | 477, 1727 |
| PaeR7I | CTCGAG | 1 cut(s) | 1539 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 192 |
| PctI | GAATGC | 1 cut(s) | 1298 |
| PfeI | GAWTC | 7 cut(s) | 197, 646, 699, 790, 907, 1124, 1563 |
| Pfl23II | CGTACG | 1 cut(s) | 597 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1418 |
| PfoI | TCCNGGA | 1 cut(s) | 1081 |
| PinAI | ACCGGT | 1 cut(s) | 289 |
| PleI | GAGTC | 5 cut(s) | 809, 1141, 1619, 1656, 2100 |
| PpsI | GAGTC | 5 cut(s) | 809, 1141, 1619, 1656, 2100 |
| PsiI | TTATAA | 2 cut(s) | 158, 1625 |
| Psp124BI | GAGCTC | 1 cut(s) | 240 |
| Psp1406I | AACGTT | 1 cut(s) | 1040 |
| Psp6I | CCWGG | 3 cut(s) | 1081, 1486, 1750 |
| PspFI | CCCAGC | 2 cut(s) | 422, 641 |
| PspGI | CCWGG | 3 cut(s) | 1081, 1486, 1750 |
| PspLI | CGTACG | 1 cut(s) | 597 |
| PspN4I | GGNNCC | 4 cut(s) | 453, 688, 1033, 1440 |
| PspPI | GGNCC | 3 cut(s) | 411, 950, 1439 |
| PspXI | VCTCGAGB | 1 cut(s) | 1539 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 257, 289, 650, 682 |
| PstI | CTGCAG | 1 cut(s) | 1231 |
| PsuI | RGATCY | 2 cut(s) | 1214, 1938 |
| PvuII | CAGCTG | 1 cut(s) | 641 |
| RsaI | GTAC | 3 cut(s) | 349, 599, 1732 |
| RsaNI | GTAC | 3 cut(s) | 348, 598, 1731 |
| RseI | CAYNNNNRTG | 2 cut(s) | 83, 435 |
| SacI | GAGCTC | 1 cut(s) | 240 |
| SaqAI | TTAA | 9 cut(s) | 249, 294, 332, 654, 972, 1241, 1574, 1745, 1944 |
| Sau3AI | GATC | 8 cut(s) | 118, 781, 877, 1214, 1827, 1844, 1921, 1938 |
| Sau96I | GGNCC | 3 cut(s) | 411, 950, 1439 |
| SchI | GAGTC | 5 cut(s) | 810, 1141, 1620, 1657, 2101 |
| ScrFI | CCNGG | 6 cut(s) | 261, 262, 1083, 1488, 1579, 1752 |
| SduI | GDGCHC | 2 cut(s) | 240, 1546 |
| SfaNI | GCATC | 3 cut(s) | 724, 902, 1349 |
| SfcI | CTRYAG | 1 cut(s) | 1227 |
| Sfr274I | CTCGAG | 1 cut(s) | 1539 |
| SinI | GGWCC | 1 cut(s) | 1439 |
| SlaI | CTCGAG | 1 cut(s) | 1539 |
| SmaI | CCCGGG | 1 cut(s) | 262 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 83, 435 |
| SmlI | CTYRAG | 3 cut(s) | 1259, 1432, 1539 |
| SmoI | CTYRAG | 3 cut(s) | 1259, 1432, 1539 |
| SsiI | CCGC | 9 cut(s) | 59, 96, 218, 690, 735, 939, 1004, 1365, 1560 |
| SspMI | CTAG | 4 cut(s) | 719, 824, 869, 1481 |
| SstI | GAGCTC | 1 cut(s) | 240 |
| StyD4I | CCNGG | 6 cut(s) | 259, 260, 1081, 1486, 1577, 1750 |
| StyI | CCWWGG | 1 cut(s) | 682 |
| TaaI | ACNGT | 7 cut(s) | 13, 105, 597, 997, 1390, 1551, 2080 |
| TaiI | ACGT | 6 cut(s) | 247, 1043, 1919, 1965, 2022, 2118 |
| TaqI | TCGA | 6 cut(s) | 338, 362, 793, 1122, 1540, 1847 |
| TaqII | GACCGA | 1 cut(s) | 854 |
| TauI | GCSGC | 1 cut(s) | 737 |
| TfiI | GAWTC | 7 cut(s) | 197, 646, 699, 790, 907, 1124, 1563 |
| Tru1I | TTAA | 9 cut(s) | 249, 294, 332, 654, 972, 1241, 1574, 1745, 1944 |
| Tru9I | TTAA | 9 cut(s) | 249, 294, 332, 654, 972, 1241, 1574, 1745, 1944 |
| TscAI | CASTG | 1 cut(s) | 2083 |
| TseFI | GTSAC | 1 cut(s) | 1799 |
| TseI | GCWGC | 9 cut(s) | 629, 638, 773, 902, 1131, 1226, 1229, 1592, 1616 |
| Tsp45I | GTSAC | 1 cut(s) | 1799 |
| TspDTI | ATGAA | 6 cut(s) | 290, 1193, 1291, 1774, 2160, 2166 |
| TspGWI | ACGGA | 2 cut(s) | 444, 990 |
| TspMI | CCCGGG | 1 cut(s) | 260 |
| TspRI | CASTG | 1 cut(s) | 2083 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1418 |
| VpaK11BI | GGWCC | 1 cut(s) | 1439 |
| XapI | RAATTY | 4 cut(s) | 334, 405, 479, 1280 |
| XbaI | TCTAGA | 1 cut(s) | 868 |
| XceI | RCATGY | 2 cut(s) | 477, 1727 |
| XhoI | CTCGAG | 1 cut(s) | 1539 |
| XmaI | CCCGGG | 1 cut(s) | 260 |
| XspI | CTAG | 4 cut(s) | 719, 824, 869, 1481 |