Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001268

Physical Location & Seq

Genomic Coordinates Reverse (-)

25948102 .. 25950375

Gene Structure 2274 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1285300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGGCTACTCTAATGCTCCTGCAAATTGTATGCACAATTGTATCTGCAATCACTCACCTCAATTCAGTTTTTGCACAATCCCATGCTTACATTATCCACATGGACTCGTCCGCCATGCCCAAAGCCTTCTCCGGCCACGAAAGGTGGTACTTAGCTACCCTTTTATCCATCTCGGACAGTGCTAAATCCTCTAATAGTACCACAAAGATCATCCATACTTATACTAATTCTATCCAAGGCTTTAGTGCAGTTCTTACACTTTCAGAACTTGAAGCCCTCAAAAAGTGCCCTGGCTTCGTTTCTTTCGCTCATGATGGCCCTCTCAAACTCCAAACTACCCACACTTCTCAGTTCCTTGGTCTAACTTCCTCTTCTGGTGCCTGGCCTGCTTCGAATTATGGCGAAGGTGTCATAATTGGTTTGCTTGATACTGGAGTTTGGCCTGAGAGCGAGAGCTTCAAGGATGAAGGAATGACTGAAGTCCCTTCTAAATGGAAAGGCAAATGTGTGTCTGGCACCCAATTCAACTCTTCCTTGTGCAACAAAAAACTCATTGGAGCTAGGTTTTTCAACAAAGGCTACATTGCCAACAATCCTGAGGAGACCATCATGATGAACTCACCCCGTGACACAGATGGACATGGAACTCACACTGCCTCCACTGCCGCCGGTCATGATGTAAAAGGCGCATCCTATTTTGGCTATGCTACTGGCACAGCTAGAGGCGCCGCACCAAGAGCTCGAATAGCTATATACAAAGTGGTTTGGTTTAACAGTGCATATAGATCCGATATACTTGCTGCTGTAGACCAAGCAATATTAGATGGGGTGGATATATTGTCAATCTCCTTAGGTTATACATTAGATGACCATTTTTTGGATGATGACACAATTGCAATAGCAACTTTTGCAGCCATGAACAGGGGAATTTTTGTTGCAGCTTCAGCAGGAAATGATGGCCCTTCGTATGGGACATTACTCAATGGAGCACCGTGGGCAGCGATAATAGGCGCAGGCACTATAGACCGCGAATTTCAAGGAATTTTAACGTTGGGAAATGGTGTTCAAATCACCTTCACTACATTGTATCCAGGCAACTCCTCTGGAAGCAAACTGCCAATTGTTTTCGCGGACGGATGTCAAAGCGTAAGGGAACTGATGAAACTCAAGAACAAGATTGTTGTGTGTAAAGACAATTTGAGCATAAGTGATCAAGTTGAAAATGCTAACTCCGCAAAAGTTTCGGGAGCTGTCTTTGTTACGAATTTTACCCTGTCAGAGTACTACACTCGAAGCTCATTTCCAGCAGCATTCATCGGTCTGAAAGATGGTGAAAATGTCATAAACTATATCAAGAAGAGTAAAAATCCCACATCAATTTTGGAATTCAAACAGACAGTACTCAGCACGAAGCCAGCACCAAAAGTAGACGATTACAGCTCTAGAGGGCCATTTCCCAGCTGCCCGAGTGTCTTGAAGCCGGACATTCTAGCCCCAGGTTCATTAATCCTGGCGGCATGGACCTCAAATAATTCAGTGGCACAAGTTCAGTCAGGCTCCTTGTTCAGCAAATTTAATGTTGTATCAGGCACTTCAATGGCTGCTCCTCATGTGGCAGGTGTGGCTGCTCTCATCAAACAAGTACACCCTGATTGGAGCCCCGCCGCGATTCGATCAGCCCTCATGACCACAGCCAATCCATTAGACAATGAACAAAATCCCATCATAGATGCTCATAGTAACCTCCCCGCAAGTCCCCTAGACATTGGAGCAGGACACATTAGTCCGAACAAGGCAATTGACCCAGGACTAATCTACGATGCAACGGCACAAGATTACATAAACCTTCTCTGTGCAATGAATTACACATCAAAAGGAATACAAGTGATCACTGGGTCCAAAAGCAATTGCATTGACCGTTCAATCCATCTTAATTACCCTTCTTTCATTGCTTACTTTAACAGTGAGGGTTCGAATTCTAGTGCAAAAGTTATACAAGAATTTAGGAGGACAGTGACAAATGTGGGAGGGGAAAAGTCTAGTTATACTGCAAAATTGACGACGATGGCGGGTTTGAAGGTGAAGGTTGAGCCACAGAGATTGGTGTTCAAGAAAAAGTATGAAAAGCTAAGTTATAAGGTCACTTTGGAAGGTTTAAAATTGTTGAAAGAAGATGTGGTCCAAGGCTCATTGATTTGGATAGAGGATGGTGGAAAATATGTTGTGAGGAGTCCCATAGTGGTCACTAACATTGTGCCAAATGATCTATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

758

Amino Acids

81.19

Weight (kDa)

8.16

Isoelectric Point (pI)

27.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	30 - 111	1.5e-14	Peptidase inhibitor I9
Peptidase_S8	PF00082	134 - 568	6.3e-48	Subtilase family
fn3_6	PF17766	644 - 748	1.3e-26	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001268)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67090
fragaria_vesca	FvH4_1g16180 FvH4_2g34170 FvH4_2g34170 FvH4_2g34230 FvH4_6g01060 FvH4_6g01061
malus_domestica	MD04G1236800.v1.1 MD04G1236900.v1.1 MD08G1098100.v1.1 MD15G1080600.v1.1 MD15G1285300.v1.1
prunus_persica	Prupe.1G433300_v2.0.a1 Prupe.6G168400_v2.0.a1 Prupe.6G356800_v2.0.a1 Prupe.7G131900_v2.0.a1
pyrus_communis	pycom04g21060 pycom04g21070 pycom08g07990 pycom12g23460 pycom15g07490 pycom15g24800
rosa_chinensis	RchiOBHm_Chr2g0105861 RchiOBHm_Chr2g0105881 RchiOBHm_Chr3g0448571 RchiOBHm_Chr6g0308191
rosa_laevigata	RLG00000010660 RLG00000017466 RLG00000017467 RLG00000025886 RLG00000025888 RLG00000025889 RLG00000025890
rosa_multiflora	Rmu_sc0003116.1_g000043 Rmu_sc0004367.1_g000007 Rmu_sc0004367.1_g000008 Rmu_sc0005706.1_g000016 Rmu_sc0010389.1_g000023
rosa_roxburghii	Rroxscaffold_2G00137780 Rroxscaffold_6G00426820 Rroxscaffold_6G00426860 Rroxscaffold_7G00160370 Rroxscaffold_7G00160460
rosa_rugosa	Rorug02G0131300 Rorug02G0131500 Rorug02G0613500 Rorug02G0613600 Rorug06G0364600
rosa_samantha	Rh2CG186700 Rh3DG013500 Rh6DG475800
rosa_wichuraiana	Rw2G014280 Rw2G014290 Rw3G000970 Rw6G041320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2136
AarI	CACCTGC	1 cut(s)	1607
AasI	GACNNNNNNGTC	1 cut(s)	1782
Acc36I	ACCTGC	1 cut(s)	1607
AccB1I	GGYRCC	3 cut(s)	377, 515, 725
AccB7I	CCANNNNNTGG	1 cut(s)	877
AccI	GTMKAC	2 cut(s)	807, 1428
AccII	CGCG	3 cut(s)	1029, 1130, 1667
AclI	AACGTT	1 cut(s)	1049
AclWI	GGATC	1 cut(s)	780
AcoI	YGGCCR	1 cut(s)	133
AcsI	RAATTY	8 cut(s)	927, 1031, 1041, 1264, 1385, 1571, 1975, 2000
AcuI	CTGAAG	2 cut(s)	498, 927
AcyI	GRCGYC	1 cut(s)	726
AdeI	CACNNNGTG	1 cut(s)	626
AfaI	GTAC	5 cut(s)	149, 199, 1283, 1401, 1644
AfiI	CCNNNNNNNGG	3 cut(s)	877, 921, 968
AjnI	CCWGG	6 cut(s)	289, 380, 1090, 1495, 1509, 1804
AloI	GAACNNNNNNTCC	2 cut(s)	1047, 1079
Alw21I	GWGCWC	2 cut(s)	742, 991
Alw26I	GTCTC	1 cut(s)	596
AlwI	GGATC	1 cut(s)	780
Ama87I	CYCGRG	1 cut(s)	1465
AoxI	GGCC	6 cut(s)	133, 316, 383, 440, 958, 1448
ApeKI	GCWGC	8 cut(s)	800, 911, 938, 998, 1307, 1461, 1601, 1625
ApoI	RAATTY	8 cut(s)	927, 1031, 1041, 1264, 1385, 1571, 1975, 2000
AseI	ATTAAT	1 cut(s)	1505
AspLEI	GCGC	3 cut(s)	689, 728, 1013
AspS9I	GGNCC	6 cut(s)	317, 959, 1448, 1521, 1896, 2179
AsuHPI	GGTGA	5 cut(s)	47, 612, 1063, 1343, 2092
AsuII	TTCGAA	2 cut(s)	392, 1973
AvaI	CYCGRG	1 cut(s)	1465
AvaII	GGWCC	3 cut(s)	1521, 1896, 2179
AxyI	CCTNAGG	2 cut(s)	597, 850
BaeGI	GKGCMC	1 cut(s)	290
BaeI	ACNNNNGTAYC	4 cut(s)	24, 57, 420, 453
BanI	GGYRCC	3 cut(s)	377, 515, 725
BanII	GRGCYC	2 cut(s)	742, 1661
Bbv12I	GWGCWC	2 cut(s)	742, 991
BbvI	GCAGC	8 cut(s)	787, 923, 950, 1010, 1319, 1448, 1588, 1612
BceAI	ACGGC	1 cut(s)	1842
BcgI	CGANNNNNNTGC	4 cut(s)	779, 813, 1224, 1258
BciT130I	CCWGG	6 cut(s)	291, 382, 1092, 1497, 1511, 1806
BciVI	GTATCC	1 cut(s)	1098
BclI	TGATCA	2 cut(s)	1210, 1887
BcoDI	GTCTC	1 cut(s)	596
BfaI	CTAG	7 cut(s)	561, 720, 1443, 1490, 1760, 1980, 2040
BfmI	CTRYAG	2 cut(s)	804, 1020
BfoI	RGCGCY	1 cut(s)	729
BfuAI	ACCTGC	1 cut(s)	1607
BfuI	GTATCC	1 cut(s)	1098
BmcAI	AGTACT	2 cut(s)	1283, 1401
Bme1390I	CCNGG	6 cut(s)	291, 382, 1092, 1497, 1511, 1806
Bme18I	GGWCC	3 cut(s)	1521, 1896, 2179
BmeT110I	CYCGRG	1 cut(s)	1465
BmgT120I	GGNCC	6 cut(s)	317, 959, 1448, 1521, 1896, 2179
BmiI	GGNNCC	6 cut(s)	379, 517, 727, 1558, 1658, 1897
BmrFI	CCNGG	6 cut(s)	291, 382, 1092, 1497, 1511, 1806
BmrI	ACTGGG	1 cut(s)	1902
BmsI	GCATC	3 cut(s)	698, 1720, 1810
BmuI	ACTGGG	1 cut(s)	1902
BoxI	GACNNNNGTC	2 cut(s)	479, 1137
BpmI	CTGGAG	1 cut(s)	453
Bpu14I	TTCGAA	2 cut(s)	392, 1973
BpuEI	CTTGAG	1 cut(s)	1151
BsaHI	GRCGYC	1 cut(s)	726
BsaI	GGTCTC	1 cut(s)	596
BsaJI	CCNNGG	7 cut(s)	235, 289, 355, 992, 1495, 1804, 2182
BsaXI	ACNNNNNCTCC	2 cut(s)	641, 671
Bsc4I	CCNNNNNNNGG	3 cut(s)	877, 921, 968
Bse118I	RCCGGY	1 cut(s)	668
Bse1I	ACTGG	3 cut(s)	436, 715, 1897
Bse21I	CCTNAGG	2 cut(s)	597, 850
Bse3DI	GCAATG	3 cut(s)	582, 1863, 1947
BseBI	CCWGG	6 cut(s)	291, 382, 1092, 1497, 1511, 1806
BseDI	CCNNGG	7 cut(s)	235, 289, 355, 992, 1495, 1804, 2182
BseGI	GGATG	6 cut(s)	210, 469, 689, 886, 1142, 2212
BseLI	CCNNNNNNNGG	3 cut(s)	877, 921, 968
BseMI	GCAATG	3 cut(s)	582, 1863, 1947
BseMII	CTCAG	4 cut(s)	362, 435, 588, 1417
BseNI	ACTGG	3 cut(s)	436, 715, 1897
BseRI	GAGGAG	4 cut(s)	614, 1090, 1596, 2242
BseSI	GKGCMC	1 cut(s)	290
BseXI	GCAGC	8 cut(s)	787, 923, 950, 1010, 1319, 1448, 1588, 1612
BseYI	CCCAGC	1 cut(s)	1457
BsgI	GTGCAG	1 cut(s)	267
Bsh1236I	CGCG	3 cut(s)	1029, 1130, 1667
BshFI	GGCC	6 cut(s)	135, 318, 385, 442, 960, 1450
BshNI	GGYRCC	3 cut(s)	377, 515, 725
BsiHKAI	GWGCWC	2 cut(s)	742, 991
BsiHKCI	CYCGRG	1 cut(s)	1465
BsiSI	CCGG	3 cut(s)	132, 669, 1481
BslFI	GGGAC	4 cut(s)	467, 985, 1740, 2217
BslI	CCNNNNNNNGG	3 cut(s)	877, 921, 968
BsmAI	GTCTC	1 cut(s)	596
BsmFI	GGGAC	4 cut(s)	467, 985, 1740, 2217
BsmI	GAATGC	1 cut(s)	1310
BsnI	GGCC	6 cut(s)	135, 318, 385, 442, 960, 1450
Bso31I	GGTCTC	1 cut(s)	596
BsoBI	CYCGRG	1 cut(s)	1465
Bsp119I	TTCGAA	2 cut(s)	392, 1973
Bsp1286I	GDGCHC	4 cut(s)	290, 742, 991, 1661
Bsp143I	GATC	6 cut(s)	207, 785, 1210, 1673, 1887, 2263
BspANI	GGCC	6 cut(s)	135, 318, 385, 442, 960, 1450
BspCNI	CTCAG	4 cut(s)	361, 436, 589, 1416
BspFNI	CGCG	3 cut(s)	1029, 1130, 1667
BspHI	TCATGA	4 cut(s)	310, 609, 673, 1683
BspLI	GGNNCC	6 cut(s)	379, 517, 727, 1558, 1658, 1897
BspMI	ACCTGC	1 cut(s)	1607
BspPI	GGATC	1 cut(s)	780
BspT104I	TTCGAA	2 cut(s)	392, 1973
BspT107I	GGYRCC	3 cut(s)	377, 515, 725
BspTNI	GGTCTC	1 cut(s)	596
BsrDI	GCAATG	3 cut(s)	582, 1863, 1947
BsrFI	RCCGGY	1 cut(s)	668
BsrI	ACTGG	3 cut(s)	436, 715, 1897
BssAI	RCCGGY	1 cut(s)	668
BssECI	CCNNGG	7 cut(s)	235, 289, 355, 992, 1495, 1804, 2182
BssMI	GATC	6 cut(s)	207, 785, 1210, 1673, 1887, 2263
BssNI	GRCGYC	1 cut(s)	726
BssT1I	CCWWGG	3 cut(s)	235, 355, 2182
Bst2UI	CCWGG	6 cut(s)	291, 382, 1092, 1497, 1511, 1806
Bst4CI	ACNGT	7 cut(s)	179, 776, 993, 1399, 1919, 1964, 2014
Bst6I	CTCTTC	3 cut(s)	376, 535, 1352
BstACI	GRCGYC	1 cut(s)	726
BstAPI	GCANNNNNTGC	1 cut(s)	908
BstBI	TTCGAA	2 cut(s)	392, 1973
BstC8I	GCNNGC	3 cut(s)	387, 1015, 1416
BstDEI	CTNAG	7 cut(s)	151, 348, 444, 597, 850, 1403, 2129
BstDSI	CCRYGG	1 cut(s)	992
BstF5I	GGATG	6 cut(s)	210, 469, 689, 886, 1142, 2212
BstFNI	CGCG	3 cut(s)	1029, 1130, 1667
BstH2I	RGCGCY	1 cut(s)	729
BstHHI	GCGC	3 cut(s)	689, 728, 1013
BstKTI	GATC	6 cut(s)	210, 788, 1213, 1676, 1890, 2266
BstMAI	GTCTC	1 cut(s)	596
BstMBI	GATC	6 cut(s)	207, 785, 1210, 1673, 1887, 2263
BstNI	CCWGG	6 cut(s)	291, 382, 1092, 1497, 1511, 1806
BstPAI	GACNNNNGTC	2 cut(s)	479, 1137
BstSCI	CCNGG	6 cut(s)	289, 380, 1090, 1495, 1509, 1804
BstSFI	CTRYAG	2 cut(s)	804, 1020
BstSLI	GKGCMC	1 cut(s)	290
BstUI	CGCG	3 cut(s)	1029, 1130, 1667
BstV1I	GCAGC	8 cut(s)	787, 923, 950, 1010, 1319, 1448, 1588, 1612
BstX2I	RGATCY	1 cut(s)	785
BstYI	RGATCY	1 cut(s)	785
Bsu36I	CCTNAGG	2 cut(s)	597, 850
BsuI	GTATCC	1 cut(s)	1098
BsuRI	GGCC	6 cut(s)	135, 318, 385, 442, 960, 1450
BtgI	CCRYGG	1 cut(s)	992
BtsCI	GGATG	6 cut(s)	210, 469, 689, 886, 1142, 2212
BtsI	GCAGTG	2 cut(s)	651, 660
BtsIMutI	CAGTG	8 cut(s)	184, 651, 660, 781, 1542, 1890, 1969, 2019
BveI	ACCTGC	1 cut(s)	1607
Cac8I	GCNNGC	3 cut(s)	387, 1015, 1416
CciI	TCATGA	4 cut(s)	310, 609, 673, 1683
CfoI	GCGC	3 cut(s)	689, 728, 1013
Cfr10I	RCCGGY	1 cut(s)	668
Cfr13I	GGNCC	6 cut(s)	317, 959, 1448, 1521, 1896, 2179
Csp6I	GTAC	5 cut(s)	148, 198, 1282, 1400, 1643
CspCI	CAANNNNNGTGG	2 cut(s)	2082, 2117
CviQI	GTAC	5 cut(s)	148, 198, 1282, 1400, 1643
DdeI	CTNAG	7 cut(s)	151, 348, 444, 597, 850, 1403, 2129
DinI	GGCGCC	1 cut(s)	727
DpnI	GATC	6 cut(s)	209, 787, 1212, 1675, 1889, 2265
DpnII	GATC	6 cut(s)	207, 785, 1210, 1673, 1887, 2263
DraI	TTTAAA	1 cut(s)	2157
DraIII	CACNNNGTG	1 cut(s)	626
DrdI	GACNNNNNNGTC	1 cut(s)	1782
DseDI	GACNNNNNNGTC	1 cut(s)	1782
EaeI	YGGCCR	1 cut(s)	133
Eam1104I	CTCTTC	3 cut(s)	376, 535, 1352
EarI	CTCTTC	3 cut(s)	376, 535, 1352
EciI	GGCGGA	1 cut(s)	100
Ecl136II	GAGCTC	1 cut(s)	740
Eco130I	CCWWGG	3 cut(s)	235, 355, 2182
Eco24I	GRGCYC	2 cut(s)	742, 1661
Eco31I	GGTCTC	1 cut(s)	596
Eco47I	GGWCC	3 cut(s)	1521, 1896, 2179
Eco53kI	GAGCTC	1 cut(s)	740
Eco57I	CTGAAG	2 cut(s)	498, 927
Eco81I	CCTNAGG	2 cut(s)	597, 850
Eco88I	CYCGRG	1 cut(s)	1465
EcoICRI	GAGCTC	1 cut(s)	740
EcoRI	GAATTC	2 cut(s)	1385, 1975
EcoRII	CCWGG	6 cut(s)	289, 380, 1090, 1495, 1509, 1804
EcoT14I	CCWWGG	3 cut(s)	235, 355, 2182
EcoT38I	GRGCYC	2 cut(s)	742, 1661
EgeI	GGCGCC	1 cut(s)	727
EheI	GGCGCC	1 cut(s)	727
ErhI	CCWWGG	3 cut(s)	235, 355, 2182
FaqI	GGGAC	4 cut(s)	467, 985, 1740, 2217
FauI	CCCGC	3 cut(s)	1669, 1756, 2062
FbaI	TGATCA	2 cut(s)	1210, 1887
FblI	GTMKAC	2 cut(s)	807, 1428
FokI	GGATG	6 cut(s)	197, 476, 676, 893, 1149, 2219
FriOI	GRGCYC	2 cut(s)	742, 1661
FspBI	CTAG	7 cut(s)	561, 720, 1443, 1490, 1760, 1980, 2040
GlaI	GCGC	3 cut(s)	688, 727, 1012
GsaI	CCCAGC	1 cut(s)	1461
GsuI	CTGGAG	1 cut(s)	453
HaeII	RGCGCY	1 cut(s)	729
HaeIII	GGCC	6 cut(s)	135, 318, 385, 442, 960, 1450
HapII	CCGG	3 cut(s)	132, 669, 1481
HhaI	GCGC	3 cut(s)	689, 728, 1013
Hin1I	GRCGYC	1 cut(s)	726
Hin6I	GCGC	3 cut(s)	687, 726, 1011
HinP1I	GCGC	3 cut(s)	687, 726, 1011
HinfI	GANTC	3 cut(s)	104, 1669, 2230
HpaII	CCGG	3 cut(s)	132, 669, 1481
HphI	GGTGA	5 cut(s)	47, 612, 1063, 1343, 2092
Hpy166II	GTNNAC	3 cut(s)	808, 1429, 1645
Hpy188I	TCNGA	6 cut(s)	175, 265, 790, 1279, 1323, 1788
Hpy8I	GTNNAC	3 cut(s)	808, 1429, 1645
Hpy99I	CGWCG	1 cut(s)	2065
HpyCH4III	ACNGT	7 cut(s)	179, 776, 993, 1399, 1919, 1964, 2014
HpyCH4IV	ACGT	1 cut(s)	1049
HpyF3I	CTNAG	7 cut(s)	151, 348, 444, 597, 850, 1403, 2129
HpySE526I	ACGT	1 cut(s)	1049
Hsp92I	GRCGYC	1 cut(s)	726
HspAI	GCGC	3 cut(s)	687, 726, 1011
KasI	GGCGCC	1 cut(s)	725
Ksp22I	TGATCA	2 cut(s)	1210, 1887
Kzo9I	GATC	6 cut(s)	207, 785, 1210, 1673, 1887, 2263
LmnI	GCTCC	8 cut(s)	21, 557, 986, 1247, 1562, 1609, 1656, 1769
Lsp1109I	GCAGC	8 cut(s)	787, 923, 950, 1010, 1319, 1448, 1588, 1612
LweI	GCATC	3 cut(s)	698, 1720, 1810
MaeI	CTAG	7 cut(s)	561, 720, 1443, 1490, 1760, 1980, 2040
MaeII	ACGT	1 cut(s)	1049
MaeIII	GTNAC	6 cut(s)	626, 1258, 1739, 2014, 2140, 2242
MalI	GATC	6 cut(s)	209, 787, 1212, 1675, 1889, 2265
MboI	GATC	6 cut(s)	207, 785, 1210, 1673, 1887, 2263
MboII	GAAGA	4 cut(s)	363, 522, 1369, 2183
MfeI	CAATTG	5 cut(s)	36, 891, 1119, 1797, 1906
MflI	RGATCY	1 cut(s)	785
MhlI	GDGCHC	4 cut(s)	290, 742, 991, 1661
Mly113I	GGCGCC	1 cut(s)	726
MlyI	GAGTC	2 cut(s)	98, 2239
MseI	TTAA	7 cut(s)	771, 1046, 1505, 1575, 1932, 1959, 2156
MslI	CAYNNNNRTG	2 cut(s)	611, 1595
MspA1I	CMGCKG	1 cut(s)	1461
MspI	CCGG	3 cut(s)	132, 669, 1481
MspR9I	CCNGG	6 cut(s)	291, 382, 1092, 1497, 1511, 1806
MunI	CAATTG	5 cut(s)	36, 891, 1119, 1797, 1906
Mva1269I	GAATGC	1 cut(s)	1310
MvaI	CCWGG	6 cut(s)	291, 382, 1092, 1497, 1511, 1806
MvnI	CGCG	3 cut(s)	1029, 1130, 1667
NarI	GGCGCC	1 cut(s)	726
NdeII	GATC	6 cut(s)	207, 785, 1210, 1673, 1887, 2263
NlaIV	GGNNCC	6 cut(s)	379, 517, 727, 1558, 1658, 1897
NmuCI	GTSAC	4 cut(s)	626, 2014, 2140, 2242
NspV	TTCGAA	2 cut(s)	392, 1973
PagI	TCATGA	4 cut(s)	310, 609, 673, 1683
PaqCI	CACCTGC	1 cut(s)	1607
PctI	GAATGC	1 cut(s)	1310
PfeI	GAWTC	1 cut(s)	1669
PflMI	CCANNNNNTGG	1 cut(s)	877
PleI	GAGTC	2 cut(s)	98, 2238
PluTI	GGCGCC	1 cut(s)	729
PpsI	GAGTC	2 cut(s)	98, 2238
PshAI	GACNNNNGTC	2 cut(s)	479, 1137
PshBI	ATTAAT	1 cut(s)	1505
PsiI	TTATAA	1 cut(s)	2136
Psp124BI	GAGCTC	1 cut(s)	742
Psp1406I	AACGTT	1 cut(s)	1049
Psp6I	CCWGG	6 cut(s)	289, 380, 1090, 1495, 1509, 1804
PspFI	CCCAGC	1 cut(s)	1457
PspGI	CCWGG	6 cut(s)	289, 380, 1090, 1495, 1509, 1804
PspN4I	GGNNCC	6 cut(s)	379, 517, 727, 1558, 1658, 1897
PspPI	GGNCC	6 cut(s)	317, 959, 1448, 1521, 1896, 2179
PsuI	RGATCY	1 cut(s)	785
PvuII	CAGCTG	1 cut(s)	1461
RsaI	GTAC	5 cut(s)	149, 199, 1283, 1401, 1644
RsaNI	GTAC	5 cut(s)	148, 198, 1282, 1400, 1643
RseI	CAYNNNNRTG	2 cut(s)	611, 1595
SacI	GAGCTC	1 cut(s)	742
SaqAI	TTAA	7 cut(s)	771, 1046, 1505, 1575, 1932, 1959, 2156
Sau3AI	GATC	6 cut(s)	207, 785, 1210, 1673, 1887, 2263
Sau96I	GGNCC	6 cut(s)	317, 959, 1448, 1521, 1896, 2179
ScaI	AGTACT	2 cut(s)	1283, 1401
SchI	GAGTC	2 cut(s)	98, 2239
ScrFI	CCNGG	6 cut(s)	291, 382, 1092, 1497, 1511, 1806
SduI	GDGCHC	4 cut(s)	290, 742, 991, 1661
SfaNI	GCATC	3 cut(s)	698, 1720, 1810
SfcI	CTRYAG	2 cut(s)	804, 1020
SfoI	GGCGCC	1 cut(s)	727
SfuI	TTCGAA	2 cut(s)	392, 1973
SinI	GGWCC	3 cut(s)	1521, 1896, 2179
SmiMI	CAYNNNNRTG	2 cut(s)	611, 1595
SmlI	CTYRAG	1 cut(s)	1166
SmoI	CTYRAG	1 cut(s)	1166
SspDI	GGCGCC	1 cut(s)	725
SspI	AATATT	1 cut(s)	819
SspMI	CTAG	7 cut(s)	561, 720, 1443, 1490, 1760, 1980, 2040
SstI	GAGCTC	1 cut(s)	742
StyD4I	CCNGG	6 cut(s)	289, 380, 1090, 1495, 1509, 1804
StyI	CCWWGG	3 cut(s)	235, 355, 2182
TaaI	ACNGT	7 cut(s)	179, 776, 993, 1399, 1919, 1964, 2014
TaiI	ACGT	1 cut(s)	1052
TaqI	TCGA	5 cut(s)	392, 742, 1291, 1672, 1973
TaqII	GACCGA	1 cut(s)	1307
TatI	WGTACW	3 cut(s)	1281, 1399, 1642
TauI	GCSGC	4 cut(s)	668, 731, 1517, 1667
TfiI	GAWTC	1 cut(s)	1669
Tru1I	TTAA	7 cut(s)	771, 1046, 1505, 1575, 1932, 1959, 2156
Tru9I	TTAA	7 cut(s)	771, 1046, 1505, 1575, 1932, 1959, 2156
TscAI	CASTG	8 cut(s)	184, 658, 667, 781, 1542, 1897, 1969, 2019
TseFI	GTSAC	4 cut(s)	626, 2014, 2140, 2242
TseI	GCWGC	8 cut(s)	800, 911, 938, 998, 1307, 1461, 1601, 1625
Tsp45I	GTSAC	4 cut(s)	626, 2014, 2140, 2242
TspGWI	ACGGA	1 cut(s)	1149
TspRI	CASTG	8 cut(s)	184, 658, 667, 781, 1542, 1897, 1969, 2019
Van91I	CCANNNNNTGG	1 cut(s)	877
VpaK11BI	GGWCC	3 cut(s)	1521, 1896, 2179
VspI	ATTAAT	1 cut(s)	1505
XapI	RAATTY	8 cut(s)	927, 1031, 1041, 1264, 1385, 1571, 1975, 2000
XbaI	TCTAGA	1 cut(s)	1442
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1378
XmiI	GTMKAC	2 cut(s)	807, 1428
XspI	CTAG	7 cut(s)	561, 720, 1443, 1490, 1760, 1980, 2040
ZrmI	AGTACT	2 cut(s)	1283, 1401

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.