Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0001268

Physical Location & Seq

Genomic Coordinates Forward (+)

75803621 .. 75807111

Gene Structure 3491 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00137780.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGGCTACTCTAGCTGTGCTAGTTCTATGCTCAATTCTAATCTCAATCATGGTTTCTGCACAATCTCACACGTACATTGTCCACATGGACTCTTCAGCCATGCCCAAAGCCTTCCTTGATCACCACAAATGGTATTTGGCAACTCTTTCATCTCTATCAGACGGTGTCAACGCTGAGGCAACTACCAGTACTACCACAAAGCTCATTTATACCTATACTAATGCCATTCAAGGCTTCAGTGCAACTCTCACACTTTCCGAGCTTGAATCCCTCAAAAGCTCCCTTGGCTTCATTTCATATATTCGTGATCGCCCCCTTAAACTTCTCACCACCCGCTCTCCTCGATTCCTTGGTCTAACACCCTTGTTTGGCGCTTGGCCTGCTTCGAATTATGGTGAGAATGTCACAATTGGTGTAATTGACACCGGAGTTTGGCCCGAGAGTGAGAGCTTCAATGATGAAGGAATGACTCAAATCCCCTCTAGATGGAAAGGAAAATGTGAATCCGGCACCCAATTTAGCTCTTCCTTGTGCAACAAGAAACTCATTGGGGCCCGGTTTTACTATAAAGGCTTTATTGCAGAGAATCCAGACTTAAATATATCAATGAAGTCAGCCCGCGATACAAGTGGACATGGAACACACACTTCCTCTATAGCTGCTGGCAATTATGTAAAGGGCGCGTCCTATTTTGGCTATGCTACTGGCACAGCTGTAGGCGTGGCACCAAGAGCTCGAATAGCAATGTACAAAGTGGGTTGGTATGAGGAGGCATCCGGATCTGATGTTATTGCTGCCGTAGACCAATCAATTCAAGATGGGGTGGACATATTATCTCTTTCCTTGGGTTATAGCTTGGATGCATATTTGTATCATGACATAATTGCAATAGCGACTTTTGCAGCCATGAATAAGGGAATTTTCGTTGCAGCTGCAGCCGGAAATGATGGCCCTGGCTACGGTACCTTAGTCAATGGAGCACCATGGGCAGTGATAGTTGGTGCAGGAACTATCGATCGCCAGCTCGGAGGAATTTTAACCTTGGGAAATGGTATAAAAATCACTTTCCCATCCTTGTATCCAAGGAACTTCTCTAGAGATACTCAGTTGCCACTTGTTTTTAATGGCTGTCAAAGTGTGATGGAACTGAAGAAACTCAAAGGCAAGATTGTTGTTTGCAAAGACAATATGAGCATAGATGTTCAAGTTGAGAATGCAAAGTCTGCAAAGGTTTCAGGAGTTGTTTTCATATCCAATACTACCTTGTCAGATTATGATACCATAACCATATTTCCAGCGGCAGTTATTGGTGACCAAGATGGCAAGCTGGTAGTAGACTATATTAAGCAAAGTAGTAATCCTAAAGCAATTTTGGAATTCAGGAAAACAGTCGTTGGTACAAAACCTGCACCAAAGGTTGACGATTACAGTTCCAGAGGGCCATTTCCAAGCTGCCCAACAATTCTGAAGCCAGACATTTTAGCCCCTGGATCATTAATATTGGCATCATGGTCCCCAACTAGTTCAGTATCTGAAGATCAGTCAAGCCCTTTGTTTAGTAACTTCAATCTTGACACAGGGACTTCAATGTCGGCACCTCATGTGGCCGGTGTGGCTGCGCTTATCAAATCTGTGCATCCTGATTGGAGTCCTGCGGCTATTAGATCAGCTCTTATGACCACAGCGAATCCATTAGACAACACTCACAGCCCTATCAAAGACGCTTACGGAAACCTCATTGCAAGCCCTTTAGCCATTGGAGCAGGACACATCAATCCCAACAAGGCTCTTGATCCAGGACTAGTCTATGATGCAGCAGCAGATGACTACATAAAGCTTCTATGTGCAATGAAATACACAGCAAAGCAGATACAAATTATCACAGGGTCCATTTATAAATGTGCCAATAGGTTCAACATTGATATTAATTACCCGTCTTTCATTGCTTACTTTAACAGTAACGGGTCAAATTCGGATGCAAAAGTTGTGCAAGAGTTCAGGAGGACACTAACAAATGTGGGAGAGGAACAGTCCAGTTACATTGTGAATCTTACAGCCGTAGCTGGTTTGAAATTGAAGGTGGAGCCACAGAGGTTGGTGTTCACAAAGAAGCATGAGAAGCTAAGGTACAAGCTGACTATGGTAGGTCCAAAATTGTTGAAAGAAGATTTGGTTCAGGGCTCACTTAGTTGGATTGATGATGGTGGAAAACATGTAGTGAGAAGTCCAATAGTGGCCACAAACATAGTGCCAGATTCTCTGATAAGGGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

757

Amino Acids

81.42

Weight (kDa)

8.35

Isoelectric Point (pI)

30.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	24 - 108	3.4e-14	Peptidase inhibitor I9
Peptidase_S8	PF00082	132 - 568	1.2e-47	Subtilase family
PA	PF02225	370 - 442	6.1e-06	PA domain
fn3_6	PF17766	641 - 745	7.3e-24	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001268)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67090
fragaria_vesca	FvH4_1g16180 FvH4_2g34170 FvH4_2g34170 FvH4_2g34230 FvH4_6g01060 FvH4_6g01061
malus_domestica	MD04G1236800.v1.1 MD04G1236900.v1.1 MD08G1098100.v1.1 MD15G1080600.v1.1 MD15G1285300.v1.1
prunus_persica	Prupe.1G433300_v2.0.a1 Prupe.6G168400_v2.0.a1 Prupe.6G356800_v2.0.a1 Prupe.7G131900_v2.0.a1
pyrus_communis	pycom04g21060 pycom04g21070 pycom08g07990 pycom12g23460 pycom15g07490 pycom15g24800
rosa_chinensis	RchiOBHm_Chr2g0105861 RchiOBHm_Chr2g0105881 RchiOBHm_Chr3g0448571 RchiOBHm_Chr6g0308191
rosa_laevigata	RLG00000010660 RLG00000017466 RLG00000017467 RLG00000025886 RLG00000025888 RLG00000025889 RLG00000025890
rosa_multiflora	Rmu_sc0003116.1_g000043 Rmu_sc0004367.1_g000007 Rmu_sc0004367.1_g000008 Rmu_sc0005706.1_g000016 Rmu_sc0010389.1_g000023
rosa_roxburghii	Rroxscaffold_2G00137780 Rroxscaffold_6G00426820 Rroxscaffold_6G00426860 Rroxscaffold_7G00160370 Rroxscaffold_7G00160460
rosa_rugosa	Rorug02G0131300 Rorug02G0131500 Rorug02G0613500 Rorug02G0613600 Rorug06G0364600
rosa_samantha	Rh2CG186700 Rh3DG013500 Rh6DG475800
rosa_wichuraiana	Rw2G014280 Rw2G014290 Rw3G000970 Rw6G041320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1896
AasI	GACNNNNNNGTC	1 cut(s)	1588
Acc36I	ACCTGC	1 cut(s)	1414
Acc65I	GGTACC	1 cut(s)	962
AccB1I	GGYRCC	4 cut(s)	509, 724, 962, 1594
AccBSI	CCGCTC	1 cut(s)	336
AccI	GTMKAC	2 cut(s)	801, 1335
AccII	CGCG	2 cut(s)	621, 683
AccIII	TCCGGA	1 cut(s)	776
AciI	CCGC	4 cut(s)	334, 619, 1298, 1655
AclWI	GGATC	3 cut(s)	787, 1498, 1787
AcoI	YGGCCR	2 cut(s)	1605, 2235
AcsI	RAATTY	4 cut(s)	918, 1032, 1376, 1969
AcuI	CTGAAG	5 cut(s)	78, 220, 1169, 1487, 1554
AfaI	GTAC	6 cut(s)	74, 190, 749, 964, 1399, 2129
AfiI	CCNNNNNNNGG	4 cut(s)	368, 432, 486, 959
AflIII	ACRYGT	2 cut(s)	69, 2212
AhlI	ACTAGT	2 cut(s)	1520, 1801
AjnI	CCWGG	3 cut(s)	952, 1486, 1795
AjuI	GAANNNNNNNTTGG	2 cut(s)	2153, 2185
Alw21I	GWGCWC	2 cut(s)	736, 982
AlwI	GGATC	3 cut(s)	787, 1498, 1787
AlwNI	CAGNNNCTG	1 cut(s)	1532
Ama87I	CYCGRG	1 cut(s)	437
Aor13HI	TCCGGA	1 cut(s)	776
AoxI	GGCC	7 cut(s)	377, 434, 552, 949, 1439, 1605, 2235
ApaI	GGGCCC	1 cut(s)	556
ApoI	RAATTY	4 cut(s)	918, 1032, 1376, 1969
AseI	ATTAAT	2 cut(s)	1496, 1926
Asp718I	GGTACC	1 cut(s)	962
AspLEI	GCGC	3 cut(s)	374, 683, 1621
AspS9I	GGNCC	8 cut(s)	435, 552, 553, 950, 1439, 1512, 1887, 2147
AsuC2I	CCSGG	1 cut(s)	556
AsuHPI	GGTGA	4 cut(s)	113, 319, 407, 1322
AsuII	TTCGAA	1 cut(s)	386
AvaI	CYCGRG	1 cut(s)	437
AvaII	GGWCC	3 cut(s)	1512, 1887, 2147
BaeGI	GKGCMC	1 cut(s)	556
BaeI	ACNNNNGTAYC	2 cut(s)	954, 987
BalI	TGGCCA	1 cut(s)	2237
BanI	GGYRCC	4 cut(s)	509, 724, 962, 1594
BanII	GRGCYC	3 cut(s)	556, 736, 2183
BarI	GAAGNNNNNNTAC	2 cut(s)	2111, 2143
Bbv12I	GWGCWC	2 cut(s)	736, 982
BbvCI	CCTCAGC	1 cut(s)	174
BccI	CCATC	7 cut(s)	480, 812, 941, 1078, 1135, 1313, 2195
BceAI	ACGGC	2 cut(s)	782, 2042
BciT130I	CCWGG	3 cut(s)	954, 1488, 1797
BciVI	GTATCC	1 cut(s)	1089
BclI	TGATCA	1 cut(s)	118
BcnI	CCSGG	1 cut(s)	556
BcuI	ACTAGT	2 cut(s)	1520, 1801
BfaI	CTAG	6 cut(s)	11, 20, 483, 1095, 1521, 1802
BfmI	CTRYAG	3 cut(s)	654, 714, 933
BfoI	RGCGCY	1 cut(s)	375
BfuAI	ACCTGC	1 cut(s)	1414
BfuI	GTATCC	1 cut(s)	1089
BglI	GCCNNNNNGGC	1 cut(s)	1613
BmcAI	AGTACT	1 cut(s)	190
Bme1390I	CCNGG	4 cut(s)	556, 954, 1488, 1797
Bme18I	GGWCC	3 cut(s)	1512, 1887, 2147
BmeT110I	CYCGRG	1 cut(s)	437
BmgT120I	GGNCC	8 cut(s)	435, 552, 553, 950, 1439, 1512, 1887, 2147
BmiI	GGNNCC	9 cut(s)	511, 553, 554, 726, 964, 1514, 1596, 1888, 2085
BmrFI	CCNGG	4 cut(s)	556, 954, 1488, 1797
BmsI	GCATC	6 cut(s)	782, 850, 1514, 1645, 1801, 1966
Bpu10I	CCTNAGC	2 cut(s)	174, 2123
Bpu14I	TTCGAA	1 cut(s)	386
BpuMI	CCSGG	1 cut(s)	556
Bsa29I	ATCGAT	1 cut(s)	1014
BsaAI	YACGTR	1 cut(s)	72
BsaJI	CCNNGG	8 cut(s)	283, 349, 843, 952, 983, 1041, 1082, 1486
BsaWI	WCCGGW	2 cut(s)	425, 776
BsaXI	ACNNNNNCTCC	2 cut(s)	322, 352
Bsc4I	CCNNNNNNNGG	4 cut(s)	368, 432, 486, 959
Bse118I	RCCGGY	1 cut(s)	1607
Bse1I	ACTGG	3 cut(s)	186, 709, 2034
Bse3DI	GCAATG	4 cut(s)	750, 1737, 1854, 1941
BseAI	TCCGGA	1 cut(s)	776
BseBI	CCWGG	3 cut(s)	954, 1488, 1797
BseCI	ATCGAT	1 cut(s)	1014
BseDI	CCNNGG	8 cut(s)	283, 349, 843, 952, 983, 1041, 1082, 1486
BseGI	GGATG	5 cut(s)	773, 865, 1070, 1636, 1981
BseLI	CCNNNNNNNGG	4 cut(s)	368, 432, 486, 959
BseMI	GCAATG	4 cut(s)	750, 1737, 1854, 1941
BseMII	CTCAG	2 cut(s)	165, 1118
BseNI	ACTGG	3 cut(s)	186, 709, 2034
BseRI	GAGGAG	2 cut(s)	330, 782
BseSI	GKGCMC	1 cut(s)	556
BsgI	GTGCAG	3 cut(s)	42, 1023, 1392
Bsh1236I	CGCG	2 cut(s)	621, 683
Bsh1285I	CGRYCG	1 cut(s)	1018
BshFI	GGCC	7 cut(s)	379, 436, 554, 951, 1441, 1607, 2237
BshNI	GGYRCC	4 cut(s)	509, 724, 962, 1594
BshVI	ATCGAT	1 cut(s)	1014
BsiEI	CGRYCG	1 cut(s)	1018
BsiHKAI	GWGCWC	2 cut(s)	736, 982
BsiHKCI	CYCGRG	1 cut(s)	437
BsiSI	CCGG	6 cut(s)	426, 507, 556, 777, 939, 1608
BslFI	GGGAC	2 cut(s)	1498, 1594
BslI	CCNNNNNNNGG	4 cut(s)	368, 432, 486, 959
BsmFI	GGGAC	2 cut(s)	1498, 1594
BsmI	GAATGC	1 cut(s)	1219
BsnI	GGCC	7 cut(s)	379, 436, 554, 951, 1441, 1607, 2237
BsoBI	CYCGRG	1 cut(s)	437
Bsp119I	TTCGAA	1 cut(s)	386
Bsp120I	GGGCCC	1 cut(s)	552
Bsp1286I	GDGCHC	4 cut(s)	556, 736, 982, 2183
Bsp13I	TCCGGA	1 cut(s)	776
Bsp1407I	TGTACA	1 cut(s)	747
Bsp143I	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
Bsp19I	CCATGG	1 cut(s)	983
BspACI	CCGC	4 cut(s)	334, 619, 1298, 1655
BspANI	GGCC	7 cut(s)	379, 436, 554, 951, 1441, 1607, 2237
BspCNI	CTCAG	2 cut(s)	166, 1117
BspDI	ATCGAT	1 cut(s)	1014
BspEI	TCCGGA	1 cut(s)	776
BspFNI	CGCG	2 cut(s)	621, 683
BspHI	TCATGA	1 cut(s)	874
BspLI	GGNNCC	9 cut(s)	511, 553, 554, 726, 964, 1514, 1596, 1888, 2085
BspMAI	CTGCAG	1 cut(s)	937
BspMI	ACCTGC	1 cut(s)	1414
BspPI	GGATC	3 cut(s)	787, 1498, 1787
BspQI	GCTCTTC	1 cut(s)	529
BspT104I	TTCGAA	1 cut(s)	386
BspT107I	GGYRCC	4 cut(s)	509, 724, 962, 1594
BsrBI	CCGCTC	1 cut(s)	336
BsrDI	GCAATG	4 cut(s)	750, 1737, 1854, 1941
BsrFI	RCCGGY	1 cut(s)	1607
BsrGI	TGTACA	1 cut(s)	747
BsrI	ACTGG	3 cut(s)	186, 709, 2034
BssAI	RCCGGY	1 cut(s)	1607
BssECI	CCNNGG	8 cut(s)	283, 349, 843, 952, 983, 1041, 1082, 1486
BssMI	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
BssT1I	CCWWGG	6 cut(s)	283, 349, 843, 983, 1041, 1082
Bst2UI	CCWGG	3 cut(s)	954, 1488, 1797
Bst4CI	ACNGT	6 cut(s)	164, 962, 1390, 1430, 1958, 2031
Bst6I	CTCTTC	2 cut(s)	97, 529
BstAPI	GCANNNNNTGC	1 cut(s)	1223
BstAUI	TGTACA	1 cut(s)	747
BstBAI	YACGTR	1 cut(s)	72
BstBI	TTCGAA	1 cut(s)	386
BstC8I	GCNNGC	6 cut(s)	381, 619, 664, 1022, 1325, 1744
BstDEI	CTNAG	5 cut(s)	174, 967, 1104, 2123, 2186
BstDSI	CCRYGG	1 cut(s)	983
BstEII	GGTNACC	1 cut(s)	1310
BstF5I	GGATG	5 cut(s)	773, 865, 1070, 1636, 1981
BstFNI	CGCG	2 cut(s)	621, 683
BstH2I	RGCGCY	1 cut(s)	375
BstHHI	GCGC	3 cut(s)	374, 683, 1621
BstKTI	GATC	8 cut(s)	121, 310, 782, 1018, 1493, 1540, 1667, 1795
BstMBI	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
BstMCI	CGRYCG	1 cut(s)	1018
BstNI	CCWGG	3 cut(s)	954, 1488, 1797
BstNSI	RCATGY	1 cut(s)	2216
BstPI	GGTNACC	1 cut(s)	1310
BstSCI	CCNGG	4 cut(s)	554, 952, 1486, 1795
BstSFI	CTRYAG	3 cut(s)	654, 714, 933
BstSLI	GKGCMC	1 cut(s)	556
BstUI	CGCG	2 cut(s)	621, 683
BstX2I	RGATCY	1 cut(s)	779
BstYI	RGATCY	1 cut(s)	779
Bsu15I	ATCGAT	1 cut(s)	1014
BsuI	GTATCC	1 cut(s)	1089
BsuRI	GGCC	7 cut(s)	379, 436, 554, 951, 1441, 1607, 2237
BsuTUI	ATCGAT	1 cut(s)	1014
BtgI	CCRYGG	1 cut(s)	983
BtsCI	GGATG	5 cut(s)	773, 865, 1070, 1636, 1981
BtsI	GCAGTG	1 cut(s)	996
BtsIMutI	CAGTG	2 cut(s)	244, 996
BveI	ACCTGC	1 cut(s)	1414
Cac8I	GCNNGC	6 cut(s)	381, 619, 664, 1022, 1325, 1744
CaiI	CAGNNNCTG	1 cut(s)	1532
CciI	TCATGA	1 cut(s)	874
CfoI	GCGC	3 cut(s)	374, 683, 1621
Cfr10I	RCCGGY	1 cut(s)	1607
Cfr13I	GGNCC	8 cut(s)	435, 552, 553, 950, 1439, 1512, 1887, 2147
ClaI	ATCGAT	1 cut(s)	1014
CseI	GACGC	2 cut(s)	672, 1730
Csp6I	GTAC	6 cut(s)	73, 189, 748, 963, 1398, 2128
CspCI	CAANNNNNGTGG	2 cut(s)	2076, 2111
CviQI	GTAC	6 cut(s)	73, 189, 748, 963, 1398, 2128
DdeI	CTNAG	5 cut(s)	174, 967, 1104, 2123, 2186
DpnI	GATC	8 cut(s)	120, 309, 781, 1017, 1492, 1539, 1666, 1794
DpnII	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
DrdI	GACNNNNNNGTC	1 cut(s)	1588
DseDI	GACNNNNNNGTC	1 cut(s)	1588
EaeI	YGGCCR	2 cut(s)	1605, 2235
Eam1104I	CTCTTC	2 cut(s)	97, 529
EarI	CTCTTC	2 cut(s)	97, 529
Ecl136II	GAGCTC	1 cut(s)	734
Eco130I	CCWWGG	6 cut(s)	283, 349, 843, 983, 1041, 1082
Eco24I	GRGCYC	3 cut(s)	556, 736, 2183
Eco47I	GGWCC	3 cut(s)	1512, 1887, 2147
Eco53kI	GAGCTC	1 cut(s)	734
Eco57I	CTGAAG	5 cut(s)	78, 220, 1169, 1487, 1554
Eco88I	CYCGRG	1 cut(s)	437
Eco91I	GGTNACC	1 cut(s)	1310
EcoICRI	GAGCTC	1 cut(s)	734
EcoO109I	RGGNCCY	1 cut(s)	552
EcoO65I	GGTNACC	1 cut(s)	1310
EcoRI	GAATTC	1 cut(s)	1376
EcoRII	CCWGG	3 cut(s)	952, 1486, 1795
EcoT14I	CCWWGG	6 cut(s)	283, 349, 843, 983, 1041, 1082
EcoT22I	ATGCAT	1 cut(s)	865
EcoT38I	GRGCYC	3 cut(s)	556, 736, 2183
ErhI	CCWWGG	6 cut(s)	283, 349, 843, 983, 1041, 1082
FalI	AAGNNNNNCTT	2 cut(s)	99, 131
FaqI	GGGAC	2 cut(s)	1498, 1594
FauI	CCCGC	2 cut(s)	341, 626
FbaI	TGATCA	1 cut(s)	118
FblI	GTMKAC	2 cut(s)	801, 1335
FokI	GGATG	5 cut(s)	760, 872, 1057, 1623, 1988
FriOI	GRGCYC	3 cut(s)	556, 736, 2183
FspBI	CTAG	6 cut(s)	11, 20, 483, 1095, 1521, 1802
GlaI	GCGC	3 cut(s)	373, 682, 1620
HaeII	RGCGCY	1 cut(s)	375
HaeIII	GGCC	7 cut(s)	379, 436, 554, 951, 1441, 1607, 2237
HapII	CCGG	6 cut(s)	426, 507, 556, 777, 939, 1608
HgaI	GACGC	2 cut(s)	672, 1730
HhaI	GCGC	3 cut(s)	374, 683, 1621
Hin6I	GCGC	3 cut(s)	372, 681, 1619
HinP1I	GCGC	3 cut(s)	372, 681, 1619
HincII	GTYRAC	2 cut(s)	169, 1420
HindII	GTYRAC	2 cut(s)	169, 1420
HindIII	AAGCTT	1 cut(s)	1835
HpaII	CCGG	6 cut(s)	426, 507, 556, 777, 939, 1608
HphI	GGTGA	4 cut(s)	113, 319, 407, 1322
Hpy166II	GTNNAC	8 cut(s)	82, 169, 632, 802, 826, 1336, 1420, 2103
Hpy188I	TCNGA	9 cut(s)	160, 259, 784, 1028, 1270, 1467, 1534, 1975, 2262
Hpy8I	GTNNAC	8 cut(s)	82, 169, 632, 802, 826, 1336, 1420, 2103
HpyAV	CCTTC	3 cut(s)	121, 455, 2071
HpyCH4III	ACNGT	6 cut(s)	164, 962, 1390, 1430, 1958, 2031
HpyCH4IV	ACGT	1 cut(s)	71
HpyF3I	CTNAG	5 cut(s)	174, 967, 1104, 2123, 2186
HpySE526I	ACGT	1 cut(s)	71
HspAI	GCGC	3 cut(s)	372, 681, 1619
Kpn2I	TCCGGA	1 cut(s)	776
KpnI	GGTACC	1 cut(s)	966
Ksp22I	TGATCA	1 cut(s)	118
Kzo9I	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
LguI	GCTCTTC	1 cut(s)	529
LmnI	GCTCC	4 cut(s)	284, 977, 1760, 2083
LweI	GCATC	6 cut(s)	782, 850, 1514, 1645, 1801, 1966
MaeI	CTAG	6 cut(s)	11, 20, 483, 1095, 1521, 1802
MaeII	ACGT	1 cut(s)	71
MaeIII	GTNAC	5 cut(s)	403, 1310, 1559, 1958, 2036
MalI	GATC	8 cut(s)	120, 309, 781, 1017, 1492, 1539, 1666, 1794
MbiI	CCGCTC	1 cut(s)	336
MboI	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
MboII	GAAGA	5 cut(s)	84, 516, 1162, 1547, 2177
MfeI	CAATTG	1 cut(s)	408
MflI	RGATCY	1 cut(s)	779
MhlI	GDGCHC	4 cut(s)	556, 736, 982, 2183
MlsI	TGGCCA	1 cut(s)	2237
MluNI	TGGCCA	1 cut(s)	2237
MlyI	GAGTC	3 cut(s)	83, 463, 1657
MmeI	TCCRAC	1 cut(s)	2171
Mox20I	TGGCCA	1 cut(s)	2237
Mph1103I	ATGCAT	1 cut(s)	865
MroI	TCCGGA	1 cut(s)	776
MscI	TGGCCA	1 cut(s)	2237
MseI	TTAA	9 cut(s)	318, 596, 1037, 1122, 1344, 1496, 1926, 1953, 2272
Msp20I	TGGCCA	1 cut(s)	2237
MspA1I	CMGCKG	3 cut(s)	713, 932, 1298
MspI	CCGG	6 cut(s)	426, 507, 556, 777, 939, 1608
MspR9I	CCNGG	4 cut(s)	556, 954, 1488, 1797
MunI	CAATTG	1 cut(s)	408
Mva1269I	GAATGC	1 cut(s)	1219
MvaI	CCWGG	3 cut(s)	954, 1488, 1797
MvnI	CGCG	2 cut(s)	621, 683
NciI	CCSGG	1 cut(s)	556
NcoI	CCATGG	1 cut(s)	983
NdeII	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
NlaIV	GGNNCC	9 cut(s)	511, 553, 554, 726, 964, 1514, 1596, 1888, 2085
NmuCI	GTSAC	2 cut(s)	403, 1310
NsiI	ATGCAT	1 cut(s)	865
NspI	RCATGY	1 cut(s)	2216
NspV	TTCGAA	1 cut(s)	386
PagI	TCATGA	1 cut(s)	874
PciI	ACATGT	1 cut(s)	2212
PciSI	GCTCTTC	1 cut(s)	529
PctI	GAATGC	1 cut(s)	1219
PfeI	GAWTC	7 cut(s)	266, 345, 503, 586, 1687, 2047, 2255
PflFI	GACNNNGTC	1 cut(s)	164
PfoI	TCCNGGA	1 cut(s)	1795
Ple19I	CGATCG	1 cut(s)	1018
PleI	GAGTC	3 cut(s)	83, 463, 1656
PpsI	GAGTC	3 cut(s)	83, 463, 1656
Ppu21I	YACGTR	1 cut(s)	72
PscI	ACATGT	1 cut(s)	2212
PshBI	ATTAAT	2 cut(s)	1496, 1926
PsiI	TTATAA	1 cut(s)	1896
Psp124BI	GAGCTC	1 cut(s)	736
Psp6I	CCWGG	3 cut(s)	952, 1486, 1795
PspEI	GGTNACC	1 cut(s)	1310
PspGI	CCWGG	3 cut(s)	952, 1486, 1795
PspN4I	GGNNCC	9 cut(s)	511, 553, 554, 726, 964, 1514, 1596, 1888, 2085
PspOMI	GGGCCC	1 cut(s)	552
PspPI	GGNCC	8 cut(s)	435, 552, 553, 950, 1439, 1512, 1887, 2147
PstI	CTGCAG	1 cut(s)	937
PstNI	CAGNNNCTG	1 cut(s)	1532
PsuI	RGATCY	1 cut(s)	779
PsyI	GACNNNGTC	1 cut(s)	164
PvuI	CGATCG	1 cut(s)	1018
PvuII	CAGCTG	2 cut(s)	713, 932
RsaI	GTAC	6 cut(s)	74, 190, 749, 964, 1399, 2129
RsaNI	GTAC	6 cut(s)	73, 189, 748, 963, 1398, 2128
SacI	GAGCTC	1 cut(s)	736
SapI	GCTCTTC	1 cut(s)	529
SaqAI	TTAA	9 cut(s)	318, 596, 1037, 1122, 1344, 1496, 1926, 1953, 2272
Sau3AI	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
Sau96I	GGNCC	8 cut(s)	435, 552, 553, 950, 1439, 1512, 1887, 2147
ScaI	AGTACT	1 cut(s)	190
SchI	GAGTC	3 cut(s)	83, 463, 1657
ScrFI	CCNGG	4 cut(s)	556, 954, 1488, 1797
SduI	GDGCHC	4 cut(s)	556, 736, 982, 2183
SfaNI	GCATC	6 cut(s)	782, 850, 1514, 1645, 1801, 1966
SfcI	CTRYAG	3 cut(s)	654, 714, 933
SfuI	TTCGAA	1 cut(s)	386
SinI	GGWCC	3 cut(s)	1512, 1887, 2147
SpeI	ACTAGT	2 cut(s)	1520, 1801
SsiI	CCGC	4 cut(s)	334, 619, 1298, 1655
SspI	AATATT	1 cut(s)	1500
SspMI	CTAG	6 cut(s)	11, 20, 483, 1095, 1521, 1802
SstI	GAGCTC	1 cut(s)	736
StyD4I	CCNGG	4 cut(s)	554, 952, 1486, 1795
StyI	CCWWGG	6 cut(s)	283, 349, 843, 983, 1041, 1082
TaaI	ACNGT	6 cut(s)	164, 962, 1390, 1430, 1958, 2031
TaiI	ACGT	1 cut(s)	74
TaqI	TCGA	4 cut(s)	343, 386, 736, 1014
TatI	WGTACW	2 cut(s)	188, 747
TauI	GCSGC	2 cut(s)	1301, 1658
TfiI	GAWTC	7 cut(s)	266, 345, 503, 586, 1687, 2047, 2255
Tru1I	TTAA	9 cut(s)	318, 596, 1037, 1122, 1344, 1496, 1926, 1953, 2272
Tru9I	TTAA	9 cut(s)	318, 596, 1037, 1122, 1344, 1496, 1926, 1953, 2272
TscAI	CASTG	2 cut(s)	244, 996
TseFI	GTSAC	2 cut(s)	403, 1310
Tsp45I	GTSAC	2 cut(s)	403, 1310
TspDTI	ATGAA	9 cut(s)	138, 280, 285, 474, 623, 923, 1237, 1865, 1930
TspGWI	ACGGA	1 cut(s)	1743
TspRI	CASTG	2 cut(s)	244, 996
Tth111I	GACNNNGTC	1 cut(s)	164
VpaK11BI	GGWCC	3 cut(s)	1512, 1887, 2147
VspI	ATTAAT	2 cut(s)	1496, 1926
XapI	RAATTY	4 cut(s)	918, 1032, 1376, 1969
XbaI	TCTAGA	2 cut(s)	482, 1094
XceI	RCATGY	1 cut(s)	2216
XmiI	GTMKAC	2 cut(s)	801, 1335
XspI	CTAG	6 cut(s)	11, 20, 483, 1095, 1521, 1802
ZrmI	AGTACT	1 cut(s)	190
Zsp2I	ATGCAT	1 cut(s)	865

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.