subtilisin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 349 |
| Acc36I | ACCTGC | 1 cut(s) | 330 |
| Acc65I | GGTACC | 1 cut(s) | 413 |
| AccB1I | GGYRCC | 3 cut(s) | 413, 736, 782 |
| AccBSI | CCGCTC | 1 cut(s) | 1778 |
| AccI | GTMKAC | 3 cut(s) | 170, 2048, 2164 |
| AccII | CGCG | 1 cut(s) | 1829 |
| AciI | CCGC | 3 cut(s) | 672, 729, 1778 |
| AclWI | GGATC | 1 cut(s) | 1221 |
| AcoI | YGGCCR | 7 cut(s) | 389, 446, 669, 687, 753, 1767, 2250 |
| AcsI | RAATTY | 2 cut(s) | 1283, 1574 |
| AcuI | CTGAAG | 2 cut(s) | 294, 2238 |
| AcvI | CACGTG | 1 cut(s) | 751 |
| AcyI | GRCGYC | 3 cut(s) | 695, 737, 927 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 297, 444, 968, 1074, 1331, 2020, 2079, 2206, 2248 |
| AflIII | ACRYGT | 1 cut(s) | 2227 |
| AgsI | TTSAA | 7 cut(s) | 1037, 1110, 1153, 1579, 1969, 1983, 2119 |
| AhlI | ACTAGT | 1 cut(s) | 1433 |
| AjnI | CCWGG | 3 cut(s) | 295, 1191, 1704 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 596, 628, 1367, 1386, 1399, 1418 |
| Alw21I | GWGCWC | 2 cut(s) | 1648, 1864 |
| Alw26I | GTCTC | 5 cut(s) | 79, 263, 312, 1408, 2174 |
| Alw44I | GTGCAC | 2 cut(s) | 1644, 1860 |
| AlwI | GGATC | 1 cut(s) | 1221 |
| Ama87I | CYCGRG | 2 cut(s) | 741, 1810 |
| ApaLI | GTGCAC | 2 cut(s) | 1644, 1860 |
| ApeKI | GCWGC | 7 cut(s) | 806, 1010, 1159, 1517, 1616, 1628, 1667 |
| ApoI | RAATTY | 2 cut(s) | 1283, 1574 |
| AseI | ATTAAT | 1 cut(s) | 1098 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1534 |
| Asp718I | GGTACC | 1 cut(s) | 413 |
| AspLEI | GCGC | 3 cut(s) | 739, 877, 1633 |
| AspS9I | GGNCC | 3 cut(s) | 886, 959, 1451 |
| AsuC2I | CCSGG | 7 cut(s) | 439, 691, 717, 1092, 1500, 1811, 1812 |
| AsuHPI | GGTGA | 6 cut(s) | 143, 1346, 1888, 2035, 2141, 2245 |
| AvaI | CYCGRG | 2 cut(s) | 741, 1810 |
| AvaII | GGWCC | 3 cut(s) | 886, 959, 1451 |
| BaeGI | GKGCMC | 3 cut(s) | 1610, 1648, 1864 |
| BalI | TGGCCA | 4 cut(s) | 448, 755, 1769, 2252 |
| BanI | GGYRCC | 3 cut(s) | 413, 736, 782 |
| BanII | GRGCYC | 1 cut(s) | 748 |
| BauI | CACGAG | 1 cut(s) | 1163 |
| BbrPI | CACGTG | 1 cut(s) | 751 |
| BbsI | GAAGAC | 2 cut(s) | 922, 1098 |
| Bbv12I | GWGCWC | 2 cut(s) | 1648, 1864 |
| BbvI | GCAGC | 7 cut(s) | 793, 1022, 1146, 1504, 1615, 1628, 1679 |
| BccI | CCATC | 6 cut(s) | 24, 492, 818, 860, 1462, 1901 |
| BceAI | ACGGC | 4 cut(s) | 421, 684, 1787, 2057 |
| BciT130I | CCWGG | 3 cut(s) | 297, 1193, 1706 |
| BclI | TGATCA | 1 cut(s) | 148 |
| BcnI | CCSGG | 7 cut(s) | 439, 691, 717, 1092, 1500, 1811, 1812 |
| BcoDI | GTCTC | 5 cut(s) | 79, 263, 312, 1408, 2174 |
| BcuI | ACTAGT | 1 cut(s) | 1433 |
| BfaI | CTAG | 5 cut(s) | 1014, 1434, 1446, 1758, 2293 |
| BfmI | CTRYAG | 2 cut(s) | 174, 1665 |
| BfoI | RGCGCY | 1 cut(s) | 740 |
| BfuAI | ACCTGC | 1 cut(s) | 330 |
| BglII | AGATCT | 1 cut(s) | 1936 |
| BisI | GCNGC | 9 cut(s) | 672, 730, 807, 1011, 1160, 1518, 1617, 1629, 1668 |
| BlpI | GCTNAGC | 1 cut(s) | 2138 |
| BlsI | GCNGC | 9 cut(s) | 673, 731, 808, 1012, 1161, 1519, 1618, 1630, 1669 |
| Bme18I | GGWCC | 3 cut(s) | 886, 959, 1451 |
| BmeT110I | CYCGRG | 2 cut(s) | 741, 1810 |
| BmgT120I | GGNCC | 3 cut(s) | 886, 959, 1451 |
| BmiI | GGNNCC | 4 cut(s) | 415, 738, 784, 888 |
| BmrI | ACTGGG | 1 cut(s) | 349 |
| BmsI | GCATC | 1 cut(s) | 1225 |
| BmuI | ACTGGG | 1 cut(s) | 349 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1737 |
| BpiI | GAAGAC | 2 cut(s) | 922, 1098 |
| BpmI | CTGGAG | 3 cut(s) | 279, 296, 1277 |
| Bpu10I | CCTNAGC | 1 cut(s) | 270 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2138 |
| BpuEI | CTTGAG | 3 cut(s) | 1229, 1463, 1989 |
| BpuMI | CCSGG | 7 cut(s) | 439, 691, 717, 1092, 1500, 1811, 1812 |
| BsaAI | YACGTR | 3 cut(s) | 685, 751, 1846 |
| BsaHI | GRCGYC | 3 cut(s) | 695, 737, 927 |
| BsaI | GGTCTC | 2 cut(s) | 312, 1408 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1053, 1083 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 297, 444, 968, 1074, 1331, 2020, 2079, 2206, 2248 |
| Bse118I | RCCGGY | 3 cut(s) | 325, 391, 1753 |
| Bse1I | ACTGG | 1 cut(s) | 355 |
| Bse3DI | GCAATG | 1 cut(s) | 1956 |
| BseBI | CCWGG | 3 cut(s) | 297, 1193, 1706 |
| BseGI | GGATG | 8 cut(s) | 108, 844, 1083, 1240, 1595, 1648, 1701, 2275 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 297, 444, 968, 1074, 1331, 2020, 2079, 2206, 2248 |
| BseMI | GCAATG | 1 cut(s) | 1956 |
| BseMII | CTCAG | 4 cut(s) | 261, 924, 2129, 2275 |
| BseNI | ACTGG | 1 cut(s) | 355 |
| BseSI | GKGCMC | 3 cut(s) | 1610, 1648, 1864 |
| BseX3I | CGGCCG | 1 cut(s) | 669 |
| BseXI | GCAGC | 7 cut(s) | 793, 1022, 1146, 1504, 1615, 1628, 1679 |
| BseYI | CCCAGC | 1 cut(s) | 1619 |
| BsgI | GTGCAG | 3 cut(s) | 1029, 1145, 1479 |
| Bsh1236I | CGCG | 1 cut(s) | 1829 |
| Bsh1285I | CGRYCG | 1 cut(s) | 672 |
| BshNI | GGYRCC | 3 cut(s) | 413, 736, 782 |
| BsiEI | CGRYCG | 1 cut(s) | 672 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1648, 1864 |
| BsiHKCI | CYCGRG | 2 cut(s) | 741, 1810 |
| BslFI | GGGAC | 6 cut(s) | 337, 647, 664, 788, 1606, 1827 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 297, 444, 968, 1074, 1331, 2020, 2079, 2206, 2248 |
| BsmAI | GTCTC | 5 cut(s) | 79, 263, 312, 1408, 2174 |
| BsmBI | CGTCTC | 1 cut(s) | 2174 |
| BsmFI | GGGAC | 6 cut(s) | 337, 647, 664, 788, 1606, 1827 |
| BsmI | GAATGC | 1 cut(s) | 1141 |
| Bso31I | GGTCTC | 2 cut(s) | 312, 1408 |
| BsoBI | CYCGRG | 2 cut(s) | 741, 1810 |
| Bsp1286I | GDGCHC | 4 cut(s) | 748, 1610, 1648, 1864 |
| Bsp1407I | TGTACA | 1 cut(s) | 759 |
| Bsp143I | GATC | 5 cut(s) | 148, 1024, 1213, 1901, 1936 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2138 |
| Bsp19I | CCATGG | 3 cut(s) | 915, 1599, 1770 |
| BspACI | CCGC | 3 cut(s) | 672, 729, 1778 |
| BspCNI | CTCAG | 4 cut(s) | 262, 923, 2130, 2274 |
| BspFNI | CGCG | 1 cut(s) | 1829 |
| BspHI | TCATGA | 2 cut(s) | 88, 1131 |
| BspLI | GGNNCC | 4 cut(s) | 415, 738, 784, 888 |
| BspMAI | CTGCAG | 1 cut(s) | 1669 |
| BspMI | ACCTGC | 1 cut(s) | 330 |
| BspPI | GGATC | 1 cut(s) | 1221 |
| BspQI | GCTCTTC | 1 cut(s) | 541 |
| BspT107I | GGYRCC | 3 cut(s) | 413, 736, 782 |
| BspTNI | GGTCTC | 2 cut(s) | 312, 1408 |
| BsrBI | CCGCTC | 1 cut(s) | 1778 |
| BsrDI | GCAATG | 1 cut(s) | 1956 |
| BsrFI | RCCGGY | 3 cut(s) | 325, 391, 1753 |
| BsrGI | TGTACA | 1 cut(s) | 759 |
| BsrI | ACTGG | 1 cut(s) | 355 |
| BssAI | RCCGGY | 3 cut(s) | 325, 391, 1753 |
| BssMI | GATC | 5 cut(s) | 148, 1024, 1213, 1901, 1936 |
| BssNI | GRCGYC | 3 cut(s) | 695, 737, 927 |
| BssSI | CACGAG | 1 cut(s) | 1163 |
| BssT1I | CCWWGG | 7 cut(s) | 81, 915, 992, 1325, 1599, 1770, 1875 |
| Bst2BI | CACGAG | 1 cut(s) | 1163 |
| Bst2UI | CCWGG | 3 cut(s) | 297, 1193, 1706 |
| Bst4CI | ACNGT | 9 cut(s) | 175, 644, 833, 1006, 1228, 1429, 1549, 2023, 2185 |
| Bst6I | CTCTTC | 3 cut(s) | 211, 541, 667 |
| BstACI | GRCGYC | 3 cut(s) | 695, 737, 927 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1244 |
| BstAUI | TGTACA | 1 cut(s) | 759 |
| BstBAI | YACGTR | 3 cut(s) | 685, 751, 1846 |
| BstC8I | GCNNGC | 4 cut(s) | 727, 819, 1606, 2062 |
| BstDEI | CTNAG | 6 cut(s) | 210, 270, 910, 1718, 2138, 2261 |
| BstDSI | CCRYGG | 3 cut(s) | 915, 1599, 1770 |
| BstEII | GGTNACC | 2 cut(s) | 2023, 2195 |
| BstF5I | GGATG | 8 cut(s) | 108, 844, 1083, 1240, 1595, 1648, 1701, 2275 |
| BstFNI | CGCG | 1 cut(s) | 1829 |
| BstH2I | RGCGCY | 1 cut(s) | 740 |
| BstHHI | GCGC | 3 cut(s) | 739, 877, 1633 |
| BstKTI | GATC | 5 cut(s) | 151, 1027, 1216, 1904, 1939 |
| BstMAI | GTCTC | 5 cut(s) | 79, 263, 312, 1408, 2174 |
| BstMBI | GATC | 5 cut(s) | 148, 1024, 1213, 1901, 1936 |
| BstMCI | CGRYCG | 1 cut(s) | 672 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 743, 752, 1244, 1625, 1775 |
| BstNI | CCWGG | 3 cut(s) | 297, 1193, 1706 |
| BstNSI | RCATGY | 3 cut(s) | 1143, 1616, 2231 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1737 |
| BstPI | GGTNACC | 2 cut(s) | 2023, 2195 |
| BstSFI | CTRYAG | 2 cut(s) | 174, 1665 |
| BstSLI | GKGCMC | 3 cut(s) | 1610, 1648, 1864 |
| BstSNI | TACGTA | 1 cut(s) | 1846 |
| BstUI | CGCG | 1 cut(s) | 1829 |
| BstV1I | GCAGC | 7 cut(s) | 793, 1022, 1146, 1504, 1615, 1628, 1679 |
| BstV2I | GAAGAC | 2 cut(s) | 922, 1098 |
| BstX2I | RGATCY | 1 cut(s) | 1936 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 362, 498, 733, 2110 |
| BstYI | RGATCY | 1 cut(s) | 1936 |
| BstZI | CGGCCG | 1 cut(s) | 669 |
| BtgI | CCRYGG | 3 cut(s) | 915, 1599, 1770 |
| BtsCI | GGATG | 8 cut(s) | 108, 844, 1083, 1240, 1595, 1648, 1701, 2275 |
| BtsIMutI | CAGTG | 2 cut(s) | 256, 2214 |
| BveI | ACCTGC | 1 cut(s) | 330 |
| Cac8I | GCNNGC | 4 cut(s) | 727, 819, 1606, 2062 |
| CciI | TCATGA | 2 cut(s) | 88, 1131 |
| CfoI | GCGC | 3 cut(s) | 739, 877, 1633 |
| Cfr10I | RCCGGY | 3 cut(s) | 325, 391, 1753 |
| Cfr13I | GGNCC | 3 cut(s) | 886, 959, 1451 |
| Cfr9I | CCCGGG | 1 cut(s) | 1810 |
| CseI | GACGC | 5 cut(s) | 479, 661, 684, 916, 1835 |
| DdeI | CTNAG | 6 cut(s) | 210, 270, 910, 1718, 2138, 2261 |
| DinI | GGCGCC | 1 cut(s) | 738 |
| DpnI | GATC | 5 cut(s) | 150, 1026, 1215, 1903, 1938 |
| DpnII | GATC | 5 cut(s) | 148, 1024, 1213, 1901, 1936 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 349 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 349 |
| EaeI | YGGCCR | 7 cut(s) | 389, 446, 669, 687, 753, 1767, 2250 |
| EagI | CGGCCG | 1 cut(s) | 669 |
| Eam1104I | CTCTTC | 3 cut(s) | 211, 541, 667 |
| EarI | CTCTTC | 3 cut(s) | 211, 541, 667 |
| EclXI | CGGCCG | 1 cut(s) | 669 |
| Eco105I | TACGTA | 1 cut(s) | 1846 |
| Eco130I | CCWWGG | 7 cut(s) | 81, 915, 992, 1325, 1599, 1770, 1875 |
| Eco147I | AGGCCT | 1 cut(s) | 281 |
| Eco24I | GRGCYC | 1 cut(s) | 748 |
| Eco31I | GGTCTC | 2 cut(s) | 312, 1408 |
| Eco47I | GGWCC | 3 cut(s) | 886, 959, 1451 |
| Eco52I | CGGCCG | 1 cut(s) | 669 |
| Eco57I | CTGAAG | 2 cut(s) | 294, 2238 |
| Eco72I | CACGTG | 1 cut(s) | 751 |
| Eco88I | CYCGRG | 2 cut(s) | 741, 1810 |
| Eco91I | GGTNACC | 2 cut(s) | 2023, 2195 |
| EcoO109I | RGGNCCY | 2 cut(s) | 886, 959 |
| EcoO65I | GGTNACC | 2 cut(s) | 2023, 2195 |
| EcoRI | GAATTC | 2 cut(s) | 1283, 1574 |
| EcoRII | CCWGG | 3 cut(s) | 295, 1191, 1704 |
| EcoT14I | CCWWGG | 7 cut(s) | 81, 915, 992, 1325, 1599, 1770, 1875 |
| EcoT22I | ATGCAT | 1 cut(s) | 1141 |
| EcoT38I | GRGCYC | 1 cut(s) | 748 |
| EgeI | GGCGCC | 1 cut(s) | 738 |
| EheI | GGCGCC | 1 cut(s) | 738 |
| ErhI | CCWWGG | 7 cut(s) | 81, 915, 992, 1325, 1599, 1770, 1875 |
| Esp3I | CGTCTC | 1 cut(s) | 2174 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 914, 946, 2126, 2158 |
| FaqI | GGGAC | 6 cut(s) | 337, 647, 664, 788, 1606, 1827 |
| FauI | CCCGC | 1 cut(s) | 1771 |
| FbaI | TGATCA | 1 cut(s) | 148 |
| FblI | GTMKAC | 3 cut(s) | 170, 2048, 2164 |
| Fnu4HI | GCNGC | 9 cut(s) | 672, 730, 807, 1011, 1160, 1518, 1617, 1629, 1668 |
| FokI | GGATG | 8 cut(s) | 95, 851, 1090, 1247, 1582, 1635, 1688, 2282 |
| FriOI | GRGCYC | 1 cut(s) | 748 |
| Fsp4HI | GCNGC | 9 cut(s) | 672, 730, 807, 1011, 1160, 1518, 1617, 1629, 1668 |
| FspBI | CTAG | 5 cut(s) | 1014, 1434, 1446, 1758, 2293 |
| GlaI | GCGC | 3 cut(s) | 738, 876, 1632 |
| GluI | GCNGC | 9 cut(s) | 672, 730, 807, 1011, 1160, 1518, 1617, 1629, 1668 |
| GsaI | CCCAGC | 1 cut(s) | 1623 |
| GsuI | CTGGAG | 3 cut(s) | 279, 296, 1277 |
| HaeII | RGCGCY | 1 cut(s) | 740 |
| HgaI | GACGC | 5 cut(s) | 479, 661, 684, 916, 1835 |
| HhaI | GCGC | 3 cut(s) | 739, 877, 1633 |
| Hin1I | GRCGYC | 3 cut(s) | 695, 737, 927 |
| Hin6I | GCGC | 3 cut(s) | 737, 875, 1631 |
| HinP1I | GCGC | 3 cut(s) | 737, 875, 1631 |
| HincII | GTYRAC | 5 cut(s) | 1552, 1807, 2049, 2165, 2225 |
| HindII | GTYRAC | 5 cut(s) | 1552, 1807, 2049, 2165, 2225 |
| HindIII | AAGCTT | 2 cut(s) | 460, 1381 |
| HpaI | GTTAAC | 2 cut(s) | 1552, 1807 |
| HphI | GGTGA | 6 cut(s) | 143, 1346, 1888, 2035, 2141, 2245 |
| Hpy188I | TCNGA | 6 cut(s) | 484, 796, 860, 1699, 2015, 2269 |
| Hpy99I | CGWCG | 6 cut(s) | 473, 644, 700, 1429, 1699, 2169 |
| HpyAV | CCTTC | 7 cut(s) | 916, 950, 1325, 1959, 2010, 2120, 2213 |
| HpyCH4III | ACNGT | 9 cut(s) | 175, 644, 833, 1006, 1228, 1429, 1549, 2023, 2185 |
| HpyCH4IV | ACGT | 6 cut(s) | 684, 750, 1694, 1845, 2055, 2147 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 743, 752, 1244, 1625, 1775 |
| HpyF3I | CTNAG | 6 cut(s) | 210, 270, 910, 1718, 2138, 2261 |
| HpySE526I | ACGT | 6 cut(s) | 684, 750, 1694, 1845, 2055, 2147 |
| Hsp92I | GRCGYC | 3 cut(s) | 695, 737, 927 |
| HspAI | GCGC | 3 cut(s) | 737, 875, 1631 |
| KasI | GGCGCC | 1 cut(s) | 736 |
| KpnI | GGTACC | 1 cut(s) | 417 |
| Ksp22I | TGATCA | 1 cut(s) | 148 |
| KspAI | GTTAAC | 2 cut(s) | 1552, 1807 |
| Kzo9I | GATC | 5 cut(s) | 148, 1024, 1213, 1901, 1936 |
| LguI | GCTCTTC | 1 cut(s) | 541 |
| LmnI | GCTCC | 3 cut(s) | 383, 718, 1775 |
| Lsp1109I | GCAGC | 7 cut(s) | 793, 1022, 1146, 1504, 1615, 1628, 1679 |
| LweI | GCATC | 1 cut(s) | 1225 |
| MaeI | CTAG | 5 cut(s) | 1014, 1434, 1446, 1758, 2293 |
| MaeII | ACGT | 6 cut(s) | 684, 750, 1694, 1845, 2055, 2147 |
| MalI | GATC | 5 cut(s) | 150, 1026, 1215, 1903, 1938 |
| MbiI | CCGCTC | 1 cut(s) | 1778 |
| MboI | GATC | 5 cut(s) | 148, 1024, 1213, 1901, 1936 |
| MfeI | CAATTG | 2 cut(s) | 1122, 1915 |
| MflI | RGATCY | 1 cut(s) | 1936 |
| MhlI | GDGCHC | 4 cut(s) | 748, 1610, 1648, 1864 |
| MlsI | TGGCCA | 4 cut(s) | 448, 755, 1769, 2252 |
| MluNI | TGGCCA | 4 cut(s) | 448, 755, 1769, 2252 |
| Mly113I | GGCGCC | 1 cut(s) | 737 |
| MlyI | GAGTC | 7 cut(s) | 365, 1075, 1330, 1669, 1809, 2103, 2253 |
| MmeI | TCCRAC | 3 cut(s) | 120, 1677, 2071 |
| Mox20I | TGGCCA | 4 cut(s) | 448, 755, 1769, 2252 |
| Mph1103I | ATGCAT | 1 cut(s) | 1141 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1534 |
| MscI | TGGCCA | 4 cut(s) | 448, 755, 1769, 2252 |
| MslI | CAYNNNNRTG | 3 cut(s) | 113, 496, 1538 |
| Msp20I | TGGCCA | 4 cut(s) | 448, 755, 1769, 2252 |
| MspA1I | CMGCKG | 3 cut(s) | 674, 729, 1619 |
| MunI | CAATTG | 2 cut(s) | 1122, 1915 |
| Mva1269I | GAATGC | 1 cut(s) | 1141 |
| MvaI | CCWGG | 3 cut(s) | 297, 1193, 1706 |
| MvnI | CGCG | 1 cut(s) | 1829 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 743, 752, 1244, 1625, 1775 |
| NarI | GGCGCC | 1 cut(s) | 737 |
| NciI | CCSGG | 7 cut(s) | 439, 691, 717, 1092, 1500, 1811, 1812 |
| NcoI | CCATGG | 3 cut(s) | 915, 1599, 1770 |
| NdeII | GATC | 5 cut(s) | 148, 1024, 1213, 1901, 1936 |
| NlaIV | GGNNCC | 4 cut(s) | 415, 738, 784, 888 |
| NmuCI | GTSAC | 6 cut(s) | 49, 607, 1028, 1142, 2023, 2065 |
| NsiI | ATGCAT | 1 cut(s) | 1141 |
| NspI | RCATGY | 3 cut(s) | 1143, 1616, 2231 |
| PagI | TCATGA | 2 cut(s) | 88, 1131 |
| PceI | AGGCCT | 1 cut(s) | 281 |
| PciI | ACATGT | 1 cut(s) | 2227 |
| PciSI | GCTCTTC | 1 cut(s) | 541 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 347, 2061 |
| PctI | GAATGC | 1 cut(s) | 1141 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1534 |
| PfeI | GAWTC | 7 cut(s) | 92, 119, 179, 585, 987, 1064, 1979 |
| PflFI | GACNNNGTC | 1 cut(s) | 1693 |
| PfoI | TCCNGGA | 2 cut(s) | 715, 1704 |
| PkrI | GCNGC | 9 cut(s) | 673, 731, 808, 1012, 1161, 1519, 1618, 1630, 1669 |
| PleI | GAGTC | 7 cut(s) | 365, 1075, 1329, 1668, 1809, 2102, 2253 |
| PluTI | GGCGCC | 1 cut(s) | 740 |
| PmaCI | CACGTG | 1 cut(s) | 751 |
| PmlI | CACGTG | 1 cut(s) | 751 |
| PpsI | GAGTC | 7 cut(s) | 365, 1075, 1329, 1668, 1809, 2102, 2253 |
| Ppu21I | YACGTR | 3 cut(s) | 685, 751, 1846 |
| PpuMI | RGGWCCY | 2 cut(s) | 886, 959 |
| PscI | ACATGT | 1 cut(s) | 2227 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1737 |
| PshBI | ATTAAT | 1 cut(s) | 1098 |
| Psp5II | RGGWCCY | 2 cut(s) | 886, 959 |
| Psp6I | CCWGG | 3 cut(s) | 295, 1191, 1704 |
| PspCI | CACGTG | 1 cut(s) | 751 |
| PspEI | GGTNACC | 2 cut(s) | 2023, 2195 |
| PspFI | CCCAGC | 1 cut(s) | 1619 |
| PspGI | CCWGG | 3 cut(s) | 295, 1191, 1704 |
| PspN4I | GGNNCC | 4 cut(s) | 415, 738, 784, 888 |
| PspPI | GGNCC | 3 cut(s) | 886, 959, 1451 |
| PspPPI | RGGWCCY | 2 cut(s) | 886, 959 |
| PstI | CTGCAG | 1 cut(s) | 1669 |
| PsuI | RGATCY | 1 cut(s) | 1936 |
| PsyI | GACNNNGTC | 1 cut(s) | 1693 |
| PvuII | CAGCTG | 1 cut(s) | 1619 |
| RseI | CAYNNNNRTG | 3 cut(s) | 113, 496, 1538 |
| SalI | GTCGAC | 2 cut(s) | 2047, 2163 |
| SapI | GCTCTTC | 1 cut(s) | 541 |
| SatI | GCNGC | 9 cut(s) | 672, 730, 807, 1011, 1160, 1518, 1617, 1629, 1668 |
| Sau3AI | GATC | 5 cut(s) | 148, 1024, 1213, 1901, 1936 |
| Sau96I | GGNCC | 3 cut(s) | 886, 959, 1451 |
| SchI | GAGTC | 7 cut(s) | 365, 1075, 1330, 1669, 1809, 2103, 2253 |
| SduI | GDGCHC | 4 cut(s) | 748, 1610, 1648, 1864 |
| SfaNI | GCATC | 1 cut(s) | 1225 |
| SfcI | CTRYAG | 2 cut(s) | 174, 1665 |
| SfoI | GGCGCC | 1 cut(s) | 738 |
| SinI | GGWCC | 3 cut(s) | 886, 959, 1451 |
| SmaI | CCCGGG | 1 cut(s) | 1812 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 113, 496, 1538 |
| SmlI | CTYRAG | 3 cut(s) | 1208, 1478, 2004 |
| SmoI | CTYRAG | 3 cut(s) | 1208, 1478, 2004 |
| SnaBI | TACGTA | 1 cut(s) | 1846 |
| SpeI | ACTAGT | 1 cut(s) | 1433 |
| SseBI | AGGCCT | 1 cut(s) | 281 |
| SsiI | CCGC | 3 cut(s) | 672, 729, 1778 |
| SspDI | GGCGCC | 1 cut(s) | 736 |
| SspMI | CTAG | 5 cut(s) | 1014, 1434, 1446, 1758, 2293 |
| StuI | AGGCCT | 1 cut(s) | 281 |
| StyI | CCWWGG | 7 cut(s) | 81, 915, 992, 1325, 1599, 1770, 1875 |
| TaaI | ACNGT | 9 cut(s) | 175, 644, 833, 1006, 1228, 1429, 1549, 2023, 2185 |
| TaiI | ACGT | 6 cut(s) | 687, 753, 1697, 1848, 2058, 2150 |
| TaqI | TCGA | 5 cut(s) | 813, 1528, 1910, 2048, 2164 |
| TatI | WGTACW | 3 cut(s) | 30, 184, 759 |
| TauI | GCSGC | 2 cut(s) | 674, 732 |
| TfiI | GAWTC | 7 cut(s) | 92, 119, 179, 585, 987, 1064, 1979 |
| TscAI | CASTG | 2 cut(s) | 256, 2221 |
| TseFI | GTSAC | 6 cut(s) | 49, 607, 1028, 1142, 2023, 2065 |
| TseI | GCWGC | 7 cut(s) | 806, 1010, 1159, 1517, 1616, 1628, 1667 |
| Tsp45I | GTSAC | 6 cut(s) | 49, 607, 1028, 1142, 2023, 2065 |
| TspGWI | ACGGA | 2 cut(s) | 1057, 1917 |
| TspMI | CCCGGG | 1 cut(s) | 1810 |
| TspRI | CASTG | 2 cut(s) | 256, 2221 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1693 |
| VneI | GTGCAC | 2 cut(s) | 1644, 1860 |
| VpaK11BI | GGWCC | 3 cut(s) | 886, 959, 1451 |
| VspI | ATTAAT | 1 cut(s) | 1098 |
| XapI | RAATTY | 2 cut(s) | 1283, 1574 |
| XbaI | TCTAGA | 1 cut(s) | 1445 |
| XceI | RCATGY | 3 cut(s) | 1143, 1616, 2231 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 424 |
| XmaI | CCCGGG | 1 cut(s) | 1810 |
| XmiI | GTMKAC | 3 cut(s) | 170, 2048, 2164 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1534 |
| XspI | CTAG | 5 cut(s) | 1014, 1434, 1446, 1758, 2293 |
| Zsp2I | ATGCAT | 1 cut(s) | 1141 |