Prupe.1G433300_v2.0.a1

subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001268
Physical Location & Seq
Forward (+)
37068979 .. 37071979
3001 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G433300.1

Sequence Viewer

Length: 2304 bp
ATGGCTATTGGTCATCATGTTCGCTTGTGTTTTTGGCTTGCCATTGCAATTATCTCCACCACTTTGGCTCAACCCAACAATTATATCATCCACATGGACTCAACCATGATGCCAAAAGCCTTTGCTGATCACCATTCATGGTATTTGGCCACAGTAAACTCTGCACTGAGCAAGTTTAGGCCAAACACCACCACAACCACCTCTTCTTCTGCCCTCTCTTCTAAACTTATTTACAGTTATACTCATGTCATAAATGGGTTCAGTGCAAGTCTCTCCCTTTCTGAGCTCGAGGCCTTAAAAACCTCTCCAGGCTACATTTCTTCAGTCAAAGACTTGCCAGTAAAACCAGACACAACTCACTCCTCTCAGTTTCTTGGCCTCAGTTCCAAATCTGGAGCTTGGCCGGTTGCAGATTATGGCAAAGATGTCATAATTGGATTGGTGGACACTGGTGTTTGGCCAGAAAGTGAGAGCTTTAGTGACGATGGAATGTCAGAAATCCCACCAAGATGGAAAGGTGAATGCGAGAGCGGCACCCAATTCAACTCCTCTTTGTGCAACAAAAAGTTGATTGGGGCAAGGTTCTTTAACAAAGGGCTCATTGCCCAAAACCCCAACTTGACATTTCCTGTGAATTCAACAAGGGACACAGATGGGCATGGGACCCACACCTCATCCACAGCAGCTGGAAATTATGTGCCCGGGGCATCTTATTTTGGTTATGCACCGGGCACAGCTAGTGGCATGGCTCCGAAGGCTCACGTGGCCATGTACAAGGCTCTTTGGGAAGAGGGCAATTTGTCTTCTGATATAATTGCTGCCATTGAGCAAGCGATTATAGACGGTGTGGATGTTTTGTCCTTGTCATTTGGGCTTGATGGGGTTGCTTTGTATGAGGACCCTGTTGCCATAGCCACATTTTCAGCCTTGGAGAAAGGTGTCTTTGTGTCCACATCTGCAGGAAATGAAGGCCCTTTCTTTGGCACCTTGCACAATGGCATCCCATGGGTTCTCACCGTTGCCGCCGCCATAATCGACCGTGATTTTGAAGGAACTGCGCATTTTGGCAACGGAGGCTCCGTCACGGGGTCTACTCTTTTTCCGGGGAATTCATCTTCAACCCAATTCCCAATTGTTTTCCTTGATGCATGTGACAGTTTGAAAAAATTGAAACAGGTGGGGAAGAAGATAGTTGTGTGCCAGGACAGAAATGATTCGTTAGGCAAACAAGTTTATAATGTTAACAACGCAACTGTTGCCGGAGGCCTTTTTATTACCGACAACACTGACTTGGAGCTGTTCCTCCAAAGCCCATTTCCCACAATCTTTCTCAGTCCAAAGGAAGGCGAAGCGATCAAAGATTACATCAACAGCAACTCTCAGCCAACAGCAAGCTTGGAATTTCAAAAGACACTTCTTGGTACCAAACCAGCACCAGTTACAACAAGCTACACCTCCAGAGGGCCATCCCCCAGCTTCCCATTTACCCTGAAGCCAGACATTCTGGCTCCAGGGTCATTGATCCTAGCTGCATGGCCTCAAAATATCGCGGTAGCCGTGGTGAACAATAAAGACTTGTTTAGTAATTTTAATCTCTTGTCCGGGACATCCATGGCGTGCCCGCATGCAGCCGGTCTAGCCGCTCTTCTAAAAGCTGCATATCCAAAATGGAGCCCTGCTGCTATTAGATCGGCCATGATGACGACATCAGACACAATGGATAACACTCTCAGCCCCATTAAAGACATTGGTGATGGTTACCAGCCAGCCAGTCCTCTAGCCATGGGAGCAGGTCATGTCAATCCCAACAAAGCCCTTGACCCGGGGCTCATATACGATGCGGAAATAGAAGATTACATAAATCTTCTTTGTGCACTGAACTACACCAACAAGCAAATCCAAACCATCACCAAATCAGCCTCGAACAATTGCTCAACTCCTTCCTTGGATCTTAACTATCCTTCTTTCATTGCATTCTTCAACGCAAACGATTCGAAGCCGAATGTACAAACTACTCAAGAATTCCGGAGGACAGTGACCTACATAGGAAAGGGACAGTCGACCTACGTTGCAAGCGTTACACCCTTAAAAGGGTTTGAGGTTGCAGTGGTTCCAAACACATTGAAGTTCAAGGAGGAGGGTGAGAAGCTGAGCTTTGTGCTGAGTATAAAAGGTCCAAGACGAACGAAGGAGACTCTGGCTTTTGGCTATCTAACTTGGGCGGAGAGTGGAGGTGAACATGTGGTGAGAAGCCCCGTCGTGGCCACAAACCTCAGCTCAGATACGGTGTCGCTGCAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

768

Amino Acids

82.0

Weight (kDa)

5.87

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1236
Acc16I TGCGCA 1 cut(s) 1059
Acc36I ACCTGC 1 cut(s) 1781
Acc65I GGTACC 1 cut(s) 1421
AccB1I GGYRCC 3 cut(s) 533, 983, 1421
AccBSI CCGCTC 2 cut(s) 531, 1643
AccI GTMKAC 2 cut(s) 1091, 2060
AccII CGCG 1 cut(s) 1550
AccIII TCCGGA 1 cut(s) 2025
AciI CCGC 8 cut(s) 531, 1023, 1026, 1550, 1622, 1641, 1841, 2222
AclWI GGATC 2 cut(s) 1516, 1956
AcoI YGGCCR 6 cut(s) 147, 401, 458, 765, 1692, 2262
AcsI RAATTY 4 cut(s) 634, 1108, 1400, 2021
AcuI CTGAAG 2 cut(s) 306, 1511
AcvI CACGTG 1 cut(s) 763
AfaI GTAC 3 cut(s) 773, 1423, 2007
AfiI CCNNNNNNNGG 8 cut(s) 980, 1086, 1343, 1461, 1822, 2090, 2091, 2260
AflIII ACRYGT 1 cut(s) 2239
AjnI CCWGG 3 cut(s) 307, 1200, 1510
AjuI GAANNNNNNNTTGG 2 cut(s) 1299, 1331
Alw21I GWGCWC 2 cut(s) 288, 1876
Alw26I GTCTC 2 cut(s) 275, 2186
Alw44I GTGCAC 1 cut(s) 1872
AlwI GGATC 2 cut(s) 1516, 1956
AlwNI CAGNNNCTG 1 cut(s) 686
Ama87I CYCGRG 3 cut(s) 287, 701, 1822
Aor13HI TCCGGA 1 cut(s) 2025
ApaLI GTGCAC 1 cut(s) 1872
ApeKI GCWGC 7 cut(s) 683, 818, 1529, 1628, 1655, 1679, 2293
ApoI RAATTY 4 cut(s) 634, 1108, 1400, 2021
ArsI GACNNNNNNTTYG 2 cut(s) 1028, 1060
Asp700I GAANNNNTTC 1 cut(s) 1213
Asp718I GGTACC 1 cut(s) 1421
AspLEI GCGC 1 cut(s) 1060
AspS9I GGNCC 5 cut(s) 663, 898, 971, 1463, 2174
AsuC2I CCSGG 7 cut(s) 702, 703, 729, 1104, 1603, 1823, 1824
AsuHPI GGTGA 9 cut(s) 122, 530, 1006, 1573, 1763, 1900, 2153, 2246, 2257
AsuII TTCGAA 1 cut(s) 1994
AvaI CYCGRG 3 cut(s) 287, 701, 1822
AvaII GGWCC 3 cut(s) 663, 898, 2174
BaeGI GKGCMC 4 cut(s) 702, 734, 1622, 1876
BalI TGGCCA 4 cut(s) 149, 460, 767, 2264
BanI GGYRCC 3 cut(s) 533, 983, 1421
BanII GRGCYC 4 cut(s) 288, 600, 1676, 1830
BbrPI CACGTG 1 cut(s) 763
BbsI GAAGAC 1 cut(s) 795
Bbv12I GWGCWC 2 cut(s) 288, 1876
BbvCI CCTCAGC 1 cut(s) 2273
BbvI GCAGC 7 cut(s) 695, 805, 1516, 1640, 1642, 1666, 2280
BccI CCATC 7 cut(s) 479, 504, 647, 872, 1474, 1748, 1913
BceAI ACGGC 1 cut(s) 1541
BcgI CGANNNNNNTGC 2 cut(s) 1974, 2008
BciT130I CCWGG 3 cut(s) 309, 1202, 1512
BclI TGATCA 1 cut(s) 127
BcnI CCSGG 7 cut(s) 702, 703, 729, 1104, 1603, 1823, 1824
BcoDI GTCTC 2 cut(s) 275, 2186
BfaI CTAG 4 cut(s) 738, 1526, 1637, 1778
BfmI CTRYAG 2 cut(s) 957, 2294
BfuAI ACCTGC 1 cut(s) 1781
BlpI GCTNAGC 1 cut(s) 2150
Bme18I GGWCC 3 cut(s) 663, 898, 2174
BmeT110I CYCGRG 3 cut(s) 287, 701, 1822
BmgT120I GGNCC 5 cut(s) 663, 898, 971, 1463, 2174
BmsI GCATC 5 cut(s) 99, 716, 1008, 1135, 1828
BpiI GAAGAC 1 cut(s) 795
BpmI CTGGAG 4 cut(s) 291, 414, 1441, 1494
Bpu10I CCTNAGC 1 cut(s) 2273
Bpu1102I GCTNAGC 1 cut(s) 2150
Bpu14I TTCGAA 1 cut(s) 1994
BpuEI CTTGAG 1 cut(s) 2001
BpuMI CCSGG 7 cut(s) 702, 703, 729, 1104, 1603, 1823, 1824
BsaAI YACGTR 1 cut(s) 763
BsaWI WCCGGW 1 cut(s) 2025
BsaXI ACNNNNNCTCC 4 cut(s) 1061, 1065, 1091, 1095
Bsc4I CCNNNNNNNGG 8 cut(s) 980, 1086, 1343, 1461, 1822, 2090, 2091, 2260
Bse118I RCCGGY 2 cut(s) 403, 1631
Bse1I ACTGG 4 cut(s) 338, 454, 1436, 1770
Bse3DI GCAATG 3 cut(s) 42, 600, 1968
BseAI TCCGGA 1 cut(s) 2025
BseBI CCWGG 3 cut(s) 309, 1202, 1512
BseGI GGATG 6 cut(s) 87, 674, 856, 999, 1466, 1607
BseLI CCNNNNNNNGG 8 cut(s) 980, 1086, 1343, 1461, 1822, 2090, 2091, 2260
BseMI GCAATG 3 cut(s) 42, 600, 1968
BseNI ACTGG 4 cut(s) 338, 454, 1436, 1770
BseRI GAGGAG 3 cut(s) 352, 538, 2150
BseSI GKGCMC 4 cut(s) 702, 734, 1622, 1876
BseXI GCAGC 7 cut(s) 695, 805, 1516, 1640, 1642, 1666, 2280
BseYI CCCAGC 1 cut(s) 1472
BsgI GTGCAG 1 cut(s) 147
Bsh1236I CGCG 1 cut(s) 1550
Bsh1285I CGRYCG 1 cut(s) 1039
BshNI GGYRCC 3 cut(s) 533, 983, 1421
BsiEI CGRYCG 1 cut(s) 1039
BsiHKAI GWGCWC 2 cut(s) 288, 1876
BsiHKCI CYCGRG 3 cut(s) 287, 701, 1822
BsiSI CCGG 9 cut(s) 404, 702, 728, 1103, 1260, 1602, 1632, 1823, 2026
BslFI GGGAC 4 cut(s) 659, 676, 1618, 2067
BslI CCNNNNNNNGG 8 cut(s) 980, 1086, 1343, 1461, 1822, 2090, 2091, 2260
BsmAI GTCTC 2 cut(s) 275, 2186
BsmFI GGGAC 4 cut(s) 659, 676, 1618, 2067
BsmI GAATGC 2 cut(s) 527, 1973
BsoBI CYCGRG 3 cut(s) 287, 701, 1822
Bsp119I TTCGAA 1 cut(s) 1994
Bsp1286I GDGCHC 8 cut(s) 288, 600, 702, 734, 1622, 1676, 1830, 1876
Bsp13I TCCGGA 1 cut(s) 2025
Bsp1407I TGTACA 2 cut(s) 771, 2005
Bsp143I GATC 5 cut(s) 127, 1353, 1521, 1688, 1948
Bsp1720I GCTNAGC 1 cut(s) 2150
Bsp19I CCATGG 3 cut(s) 1004, 1611, 1782
BspACI CCGC 8 cut(s) 531, 1023, 1026, 1550, 1622, 1641, 1841, 2222
BspEI TCCGGA 1 cut(s) 2025
BspFNI CGCG 1 cut(s) 1550
BspMAI CTGCAG 2 cut(s) 961, 2298
BspMI ACCTGC 1 cut(s) 1781
BspPI GGATC 2 cut(s) 1516, 1956
BspQI GCTCTTC 1 cut(s) 1650
BspT104I TTCGAA 1 cut(s) 1994
BspT107I GGYRCC 3 cut(s) 533, 983, 1421
BsrBI CCGCTC 2 cut(s) 531, 1643
BsrDI GCAATG 3 cut(s) 42, 600, 1968
BsrFI RCCGGY 2 cut(s) 403, 1631
BsrGI TGTACA 2 cut(s) 771, 2005
BsrI ACTGG 4 cut(s) 338, 454, 1436, 1770
BssAI RCCGGY 2 cut(s) 403, 1631
BssMI GATC 5 cut(s) 127, 1353, 1521, 1688, 1948
BssT1I CCWWGG 5 cut(s) 927, 1004, 1611, 1782, 1944
Bst2UI CCWGG 3 cut(s) 309, 1202, 1512
Bst6I CTCTTC 4 cut(s) 208, 223, 783, 1650
BstAPI GCANNNNNTGC 1 cut(s) 1256
BstAUI TGTACA 2 cut(s) 771, 2005
BstBAI YACGTR 1 cut(s) 763
BstBI TTCGAA 1 cut(s) 1994
BstC8I GCNNGC 8 cut(s) 39, 831, 1393, 1618, 1622, 1626, 1767, 2074
BstDSI CCRYGG 4 cut(s) 1004, 1557, 1611, 1782
BstEII GGTNACC 1 cut(s) 1757
BstF5I GGATG 6 cut(s) 87, 674, 856, 999, 1466, 1607
BstFNI CGCG 1 cut(s) 1550
BstHHI GCGC 1 cut(s) 1060
BstKTI GATC 5 cut(s) 130, 1356, 1524, 1691, 1951
BstMAI GTCTC 2 cut(s) 275, 2186
BstMBI GATC 5 cut(s) 127, 1353, 1521, 1688, 1948
BstMCI CGRYCG 1 cut(s) 1039
BstMWI GCNNNNNNNGC 7 cut(s) 531, 755, 764, 1074, 1256, 1637, 1787
BstNI CCWGG 3 cut(s) 309, 1202, 1512
BstNSI RCATGY 3 cut(s) 1152, 1628, 2243
BstPI GGTNACC 1 cut(s) 1757
BstSFI CTRYAG 2 cut(s) 957, 2294
BstSLI GKGCMC 4 cut(s) 702, 734, 1622, 1876
BstUI CGCG 1 cut(s) 1550
BstV1I GCAGC 7 cut(s) 695, 805, 1516, 1640, 1642, 1666, 2280
BstV2I GAAGAC 1 cut(s) 795
BstX2I RGATCY 1 cut(s) 1948
BstXI CCANNNNNNTGG 2 cut(s) 64, 510
BstYI RGATCY 1 cut(s) 1948
BtgI CCRYGG 4 cut(s) 1004, 1557, 1611, 1782
BtsCI GGATG 6 cut(s) 87, 674, 856, 999, 1466, 1607
BtsI GCAGTG 1 cut(s) 2112
BtsIMutI CAGTG 7 cut(s) 164, 268, 447, 1284, 1874, 2040, 2112
BveI ACCTGC 1 cut(s) 1781
Cac8I GCNNGC 8 cut(s) 39, 831, 1393, 1618, 1622, 1626, 1767, 2074
CaiI CAGNNNCTG 1 cut(s) 686
CfoI GCGC 1 cut(s) 1060
Cfr10I RCCGGY 2 cut(s) 403, 1631
Cfr13I GGNCC 5 cut(s) 663, 898, 971, 1463, 2174
Cfr9I CCCGGG 2 cut(s) 701, 1822
Csp6I GTAC 3 cut(s) 772, 1422, 2006
CviQI GTAC 3 cut(s) 772, 1422, 2006
DpnI GATC 5 cut(s) 129, 1355, 1523, 1690, 1950
DpnII GATC 5 cut(s) 127, 1353, 1521, 1688, 1948
EaeI YGGCCR 6 cut(s) 147, 401, 458, 765, 1692, 2262
Eam1104I CTCTTC 4 cut(s) 208, 223, 783, 1650
EarI CTCTTC 4 cut(s) 208, 223, 783, 1650
EciI GGCGGA 1 cut(s) 2237
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 5 cut(s) 927, 1004, 1611, 1782, 1944
Eco147I AGGCCT 2 cut(s) 293, 1266
Eco24I GRGCYC 4 cut(s) 288, 600, 1676, 1830
Eco47I GGWCC 3 cut(s) 663, 898, 2174
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 2 cut(s) 306, 1511
Eco72I CACGTG 1 cut(s) 763
Eco88I CYCGRG 3 cut(s) 287, 701, 1822
Eco91I GGTNACC 1 cut(s) 1757
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 3 cut(s) 663, 898, 971
EcoO65I GGTNACC 1 cut(s) 1757
EcoRI GAATTC 3 cut(s) 634, 1108, 2021
EcoRII CCWGG 3 cut(s) 307, 1200, 1510
EcoT14I CCWWGG 5 cut(s) 927, 1004, 1611, 1782, 1944
EcoT22I ATGCAT 1 cut(s) 1150
EcoT38I GRGCYC 4 cut(s) 288, 600, 1676, 1830
ErhI CCWWGG 5 cut(s) 927, 1004, 1611, 1782, 1944
FalI AAGNNNNNCTT 2 cut(s) 2138, 2170
FaqI GGGAC 4 cut(s) 659, 676, 1618, 2067
FauI CCCGC 1 cut(s) 1629
FbaI TGATCA 1 cut(s) 127
FblI GTMKAC 2 cut(s) 1091, 2060
FokI GGATG 6 cut(s) 74, 661, 863, 986, 1453, 1594
FriOI GRGCYC 4 cut(s) 288, 600, 1676, 1830
FspBI CTAG 4 cut(s) 738, 1526, 1637, 1778
FspI TGCGCA 1 cut(s) 1059
GlaI GCGC 1 cut(s) 1059
GsaI CCCAGC 1 cut(s) 1476
GsuI CTGGAG 4 cut(s) 291, 414, 1441, 1494
HapII CCGG 9 cut(s) 404, 702, 728, 1103, 1260, 1602, 1632, 1823, 2026
HhaI GCGC 1 cut(s) 1060
Hin6I GCGC 1 cut(s) 1058
HinP1I GCGC 1 cut(s) 1058
HincII GTYRAC 2 cut(s) 1243, 2061
HindII GTYRAC 2 cut(s) 1243, 2061
HindIII AAGCTT 1 cut(s) 1393
HinfI GANTC 4 cut(s) 98, 1214, 1991, 2194
HpaI GTTAAC 1 cut(s) 1243
HpaII CCGG 9 cut(s) 404, 702, 728, 1103, 1260, 1602, 1632, 1823, 2026
HphI GGTGA 9 cut(s) 122, 530, 1006, 1573, 1763, 1900, 2153, 2246, 2257
Hpy166II GTNNAC 9 cut(s) 157, 445, 951, 1092, 1243, 1564, 1874, 2061, 2237
Hpy188I TCNGA 6 cut(s) 283, 496, 753, 808, 1711, 2281
Hpy188III TCNNGA 4 cut(s) 393, 1458, 2018, 2026
Hpy8I GTNNAC 9 cut(s) 157, 445, 951, 1092, 1243, 1564, 1874, 2061, 2237
Hpy99I CGWCG 1 cut(s) 2261
HpyAV CCTTC 7 cut(s) 748, 962, 1043, 1337, 1950, 1971, 2182
HpyCH4IV ACGT 2 cut(s) 762, 2067
HpyF10VI GCNNNNNNNGC 7 cut(s) 531, 755, 764, 1074, 1256, 1637, 1787
HpySE526I ACGT 2 cut(s) 762, 2067
HspAI GCGC 1 cut(s) 1058
KflI GGGWCCC 1 cut(s) 663
Kpn2I TCCGGA 1 cut(s) 2025
KpnI GGTACC 1 cut(s) 1425
Ksp22I TGATCA 1 cut(s) 127
KspAI GTTAAC 1 cut(s) 1243
Kzo9I GATC 5 cut(s) 127, 1353, 1521, 1688, 1948
LguI GCTCTTC 1 cut(s) 1650
LmnI GCTCC 7 cut(s) 395, 754, 1082, 1294, 1513, 1671, 1787
Lsp1109I GCAGC 7 cut(s) 695, 805, 1516, 1640, 1642, 1666, 2280
LweI GCATC 5 cut(s) 99, 716, 1008, 1135, 1828
MaeI CTAG 4 cut(s) 738, 1526, 1637, 1778
MaeII ACGT 2 cut(s) 762, 2067
MaeIII GTNAC 7 cut(s) 479, 1081, 1151, 1438, 1757, 2035, 2077
MalI GATC 5 cut(s) 129, 1355, 1523, 1690, 1950
MbiI CCGCTC 2 cut(s) 531, 1643
MboI GATC 5 cut(s) 127, 1353, 1521, 1688, 1948
MfeI CAATTG 2 cut(s) 1131, 1927
MflI RGATCY 1 cut(s) 1948
MhlI GDGCHC 8 cut(s) 288, 600, 702, 734, 1622, 1676, 1830, 1876
MlsI TGGCCA 4 cut(s) 149, 460, 767, 2264
MluNI TGGCCA 4 cut(s) 149, 460, 767, 2264
MlyI GAGTC 2 cut(s) 92, 2188
Mox20I TGGCCA 4 cut(s) 149, 460, 767, 2264
Mph1103I ATGCAT 1 cut(s) 1150
MroI TCCGGA 1 cut(s) 2025
MroXI GAANNNNTTC 1 cut(s) 1213
MscI TGGCCA 4 cut(s) 149, 460, 767, 2264
MseI TTAA 7 cut(s) 296, 588, 1242, 1590, 1740, 1953, 2087
MslI CAYNNNNRTG 2 cut(s) 92, 508
Msp20I TGGCCA 4 cut(s) 149, 460, 767, 2264
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 9 cut(s) 404, 702, 728, 1103, 1260, 1602, 1632, 1823, 2026
MunI CAATTG 2 cut(s) 1131, 1927
Mva1269I GAATGC 2 cut(s) 527, 1973
MvaI CCWGG 3 cut(s) 309, 1202, 1512
MvnI CGCG 1 cut(s) 1550
MwoI GCNNNNNNNGC 7 cut(s) 531, 755, 764, 1074, 1256, 1637, 1787
NciI CCSGG 7 cut(s) 702, 703, 729, 1104, 1603, 1823, 1824
NcoI CCATGG 3 cut(s) 1004, 1611, 1782
NdeII GATC 5 cut(s) 127, 1353, 1521, 1688, 1948
NmuCI GTSAC 4 cut(s) 479, 1081, 1151, 2035
NsbI TGCGCA 1 cut(s) 1059
NsiI ATGCAT 1 cut(s) 1150
NspI RCATGY 3 cut(s) 1152, 1628, 2243
NspV TTCGAA 1 cut(s) 1994
PaeI GCATGC 1 cut(s) 1628
PaeR7I CTCGAG 1 cut(s) 287
PceI AGGCCT 2 cut(s) 293, 1266
PciI ACATGT 1 cut(s) 2239
PciSI GCTCTTC 1 cut(s) 1650
PcsI WCGNNNNNNNCGW 3 cut(s) 1077, 1554, 2073
PctI GAATGC 2 cut(s) 527, 1973
PdmI GAANNNNTTC 1 cut(s) 1213
PfeI GAWTC 2 cut(s) 1214, 1991
PfoI TCCNGGA 1 cut(s) 1601
PleI GAGTC 2 cut(s) 92, 2188
PmaCI CACGTG 1 cut(s) 763
PmlI CACGTG 1 cut(s) 763
PpsI GAGTC 2 cut(s) 92, 2188
Ppu21I YACGTR 1 cut(s) 763
PpuMI RGGWCCY 2 cut(s) 663, 898
PscI ACATGT 1 cut(s) 2239
PsiI TTATAA 1 cut(s) 1236
Psp124BI GAGCTC 1 cut(s) 288
Psp5II RGGWCCY 2 cut(s) 663, 898
Psp6I CCWGG 3 cut(s) 307, 1200, 1510
PspCI CACGTG 1 cut(s) 763
PspEI GGTNACC 1 cut(s) 1757
PspFI CCCAGC 1 cut(s) 1472
PspGI CCWGG 3 cut(s) 307, 1200, 1510
PspPI GGNCC 5 cut(s) 663, 898, 971, 1463, 2174
PspPPI RGGWCCY 2 cut(s) 663, 898
PspXI VCTCGAGB 1 cut(s) 287
PstI CTGCAG 2 cut(s) 961, 2298
PstNI CAGNNNCTG 1 cut(s) 686
PsuI RGATCY 1 cut(s) 1948
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 3 cut(s) 773, 1423, 2007
RsaNI GTAC 3 cut(s) 772, 1422, 2006
RseI CAYNNNNRTG 2 cut(s) 92, 508
SacI GAGCTC 1 cut(s) 288
SalI GTCGAC 1 cut(s) 2059
SapI GCTCTTC 1 cut(s) 1650
SaqAI TTAA 7 cut(s) 296, 588, 1242, 1590, 1740, 1953, 2087
Sau3AI GATC 5 cut(s) 127, 1353, 1521, 1688, 1948
Sau96I GGNCC 5 cut(s) 663, 898, 971, 1463, 2174
SchI GAGTC 2 cut(s) 92, 2188
SduI GDGCHC 8 cut(s) 288, 600, 702, 734, 1622, 1676, 1830, 1876
SfaNI GCATC 5 cut(s) 99, 716, 1008, 1135, 1828
SfcI CTRYAG 2 cut(s) 957, 2294
Sfr274I CTCGAG 1 cut(s) 287
SfuI TTCGAA 1 cut(s) 1994
SinI GGWCC 3 cut(s) 663, 898, 2174
SlaI CTCGAG 1 cut(s) 287
SmaI CCCGGG 2 cut(s) 703, 1824
SmiMI CAYNNNNRTG 2 cut(s) 92, 508
SmlI CTYRAG 2 cut(s) 287, 2016
SmoI CTYRAG 2 cut(s) 287, 2016
SphI GCATGC 1 cut(s) 1628
SseBI AGGCCT 2 cut(s) 293, 1266
SsiI CCGC 8 cut(s) 531, 1023, 1026, 1550, 1622, 1641, 1841, 2222
SspMI CTAG 4 cut(s) 738, 1526, 1637, 1778
SstI GAGCTC 1 cut(s) 288
StuI AGGCCT 2 cut(s) 293, 1266
StyI CCWWGG 5 cut(s) 927, 1004, 1611, 1782, 1944
TaiI ACGT 2 cut(s) 765, 2070
TaqI TCGA 5 cut(s) 288, 1035, 1922, 1994, 2060
TatI WGTACW 2 cut(s) 771, 2005
TauI GCSGC 4 cut(s) 534, 1025, 1028, 1643
TfiI GAWTC 2 cut(s) 1214, 1991
Tru1I TTAA 7 cut(s) 296, 588, 1242, 1590, 1740, 1953, 2087
Tru9I TTAA 7 cut(s) 296, 588, 1242, 1590, 1740, 1953, 2087
TscAI CASTG 7 cut(s) 171, 268, 454, 1291, 1881, 2040, 2112
TseFI GTSAC 4 cut(s) 479, 1081, 1151, 2035
TseI GCWGC 7 cut(s) 683, 818, 1529, 1628, 1655, 1679, 2293
Tsp45I GTSAC 4 cut(s) 479, 1081, 1151, 2035
TspDTI ATGAA 4 cut(s) 126, 981, 1101, 1957
TspGWI ACGGA 2 cut(s) 1069, 1086
TspMI CCCGGG 2 cut(s) 701, 1822
TspRI CASTG 7 cut(s) 171, 268, 454, 1291, 1881, 2040, 2112
VneI GTGCAC 1 cut(s) 1872
VpaK11BI GGWCC 3 cut(s) 663, 898, 2174
XapI RAATTY 4 cut(s) 634, 1108, 1400, 2021
XceI RCATGY 3 cut(s) 1152, 1628, 2243
XhoI CTCGAG 1 cut(s) 287
XmaI CCCGGG 2 cut(s) 701, 1822
XmiI GTMKAC 2 cut(s) 1091, 2060
XmnI GAANNNNTTC 1 cut(s) 1213
XspI CTAG 4 cut(s) 738, 1526, 1637, 1778
Zsp2I ATGCAT 1 cut(s) 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.