RLG00000025889

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001268
Physical Location & Seq
Forward (+)
49002681 .. 49004021
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025889

Sequence Viewer

Length: 1341 bp
ATGGAGAAGAATGTAGTAGTTTCATCTTCAGCCGGAAATGAAGTCACAAAGGGCCTTGGGAAATTGCACAATGGCATCCCTTGGGCCCTGTCTGTTGCAGCTGGCACGATTGACCGCTCATTTGGTGGAACATTAAGTCTTGGTAATGGTTTGACCGTTCCTGGATTTACCTTGTTCCTTGGAAATGCTCTGATAAATAAGCTTCCACTGGTTTACAACCAGACATTATCAGCTTGCAACGACACAGAACTATTATATACTAGCCCCAATGCAATCCTTATATGTGATGACATTTCCGATATCCGTGGTCAAATATCTCATATCATTGCTGCAGAGGTGCTTGGAGCTGTCTTTGTTTCGAACGATCCTGATATTTATGCATTAGAAGGTTTTAGAACTCCTATTGTTGTGGTAAGCCCAGATGATGCACCAGCTCTATTCAAATATGCAAAGAGGGCTGACCCTATCGTTAGCATGACATTCCAACAAACATTCATTGGGACAAAGCCTGCACCGGCGGCTGCATTTTATTCTTCAAGAGGTCCTTCATCAAGTTATCTGGGTATCTTAAAGCCGGACATAATGACACCGGGGTCATTAGTTTTGGCTGCTTGGCCTCCGAATGTAGCAGCTGCCAGAATTGGTGGAAATGTGATGTTACCAAGTGACTATAATTTGATATCTGGAACATCCATGGCTTGCCCTCATGCTTCAGGCGTAGCTGCACTACTAAAAGGCGCACACCCTGAATGGAGTGCAGCTGCCATTAGGTCTGCTTTGGTCACCACAGCCAACCCATTAGACAATACTCAGAATTCGATTCGTGACAATGGTGACAATTTCAACTATGCTTCACCTCTAGCTATGGGAGCAGGCCAGATTGATCCTAATAGAGCACTTGAACCTGGTTTGATATATGATGCAACCCCACAAGACTATGTCAATCTCTTGTGCTCTACAAATTTTACCCGTAAGCAAATCTTAGCCATCACTAGATCACGTGCCTATGATTGTTCCAGCCCATCGTCTGATCTGAACTATCCCTCCTTCATTGCTTTGTATGATCCTCGTCATCACACAAGGAAACTAGGGGTCCAAAAATTTCAGAGGATAGTAACAACTGTGGGAGATGGTGCAGCTAAGTACAAGGCTTCGGTGACAGCTCCAAAGGGTTCTATAGTAACAGTGTCACCGGAGACATTGTTCTTTTCTGAGACATATGAGAAGCGAAGCTACACTCTTACTATAAACTACAAGGGAGACAAGAAAGGAAATGTGTCATTTGGGAACTTGTTTGAGTGGAAGAAAATGGAAAGTACAATGTCAGGAGCCCCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

447

Amino Acids

47.59

Weight (kDa)

8.14

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 2 - 271 3.5e-20 Subtilase family
fn3_6 PF17766 344 - 436 2e-20 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 592
AccBSI CCGCTC 1 cut(s) 117
AciI CCGC 2 cut(s) 115, 518
AclWI GGATC 3 cut(s) 359, 878, 1058
AcsI RAATTY 3 cut(s) 814, 961, 1100
AcuI CTGAAG 2 cut(s) 12, 696
AcvI CACGTG 1 cut(s) 1001
AfaI GTAC 2 cut(s) 1145, 1318
AgsI TTSAA 4 cut(s) 442, 537, 844, 902
AjnI CCWGG 2 cut(s) 160, 904
AloI GAACNNNNNNTCC 4 cut(s) 1028, 1060, 1187, 1219
Alw21I GWGCWC 2 cut(s) 898, 956
Alw26I GTCTC 3 cut(s) 1190, 1208, 1254
AlwI GGATC 3 cut(s) 359, 878, 1058
AoxI GGCC 4 cut(s) 52, 84, 614, 874
ApaI GGGCCC 1 cut(s) 88
ApoI RAATTY 3 cut(s) 814, 961, 1100
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AspLEI GCGC 1 cut(s) 740
AspS9I GGNCC 5 cut(s) 52, 84, 85, 542, 1093
AsuC2I CCSGG 1 cut(s) 591
AsuHPI GGTGA 5 cut(s) 775, 845, 846, 1168, 1182
AsuII TTCGAA 1 cut(s) 359
AvaII GGWCC 2 cut(s) 542, 1093
BaeGI GKGCMC 1 cut(s) 88
BanII GRGCYC 2 cut(s) 88, 1333
BarI GAAGNNNNNNTAC 2 cut(s) 1217, 1249
BbrPI CACGTG 1 cut(s) 1001
Bbv12I GWGCWC 2 cut(s) 898, 956
BccI CCATC 3 cut(s) 995, 1030, 1124
BciT130I CCWGG 2 cut(s) 162, 906
BcnI CCSGG 1 cut(s) 591
BcoDI GTCTC 3 cut(s) 1190, 1208, 1254
BfaI CTAG 4 cut(s) 261, 860, 993, 1088
BfmI CTRYAG 2 cut(s) 330, 1176
Bme1390I CCNGG 3 cut(s) 162, 591, 906
Bme18I GGWCC 2 cut(s) 542, 1093
BmgT120I GGNCC 5 cut(s) 52, 84, 85, 542, 1093
BmiI GGNNCC 3 cut(s) 86, 1094, 1330
BmrFI CCNGG 3 cut(s) 162, 591, 906
BmsI GCATC 3 cut(s) 84, 415, 910
Bpu14I TTCGAA 1 cut(s) 359
BpuMI CCSGG 1 cut(s) 591
BsaAI YACGTR 1 cut(s) 1001
BsaJI CCNNGG 6 cut(s) 55, 80, 178, 304, 590, 693
BsaWI WCCGGW 1 cut(s) 1192
BsaXI ACNNNNNCTCC 4 cut(s) 1028, 1058, 1187, 1217
Bse118I RCCGGY 1 cut(s) 514
Bse1I ACTGG 1 cut(s) 213
Bse3DI GCAATG 2 cut(s) 324, 1050
BseBI CCWGG 2 cut(s) 162, 906
BseDI CCNNGG 6 cut(s) 55, 80, 178, 304, 590, 693
BseGI GGATG 2 cut(s) 75, 689
BseMI GCAATG 2 cut(s) 324, 1050
BseMII CTCAG 2 cut(s) 824, 1203
BseNI ACTGG 1 cut(s) 213
BseSI GKGCMC 1 cut(s) 88
BsgI GTGCAG 4 cut(s) 495, 708, 777, 1155
BshFI GGCC 4 cut(s) 54, 86, 616, 876
BsiHKAI GWGCWC 2 cut(s) 898, 956
BsiSI CCGG 5 cut(s) 33, 515, 575, 590, 1193
BslFI GGGAC 1 cut(s) 514
BsmAI GTCTC 3 cut(s) 1190, 1208, 1254
BsmFI GGGAC 1 cut(s) 514
BsnI GGCC 4 cut(s) 54, 86, 616, 876
Bsp119I TTCGAA 1 cut(s) 359
Bsp120I GGGCCC 1 cut(s) 84
Bsp1286I GDGCHC 4 cut(s) 88, 898, 956, 1333
Bsp143I GATC 5 cut(s) 364, 883, 995, 1030, 1063
Bsp19I CCATGG 1 cut(s) 693
BspACI CCGC 2 cut(s) 115, 518
BspANI GGCC 4 cut(s) 54, 86, 616, 876
BspCNI CTCAG 2 cut(s) 823, 1204
BspLI GGNNCC 3 cut(s) 86, 1094, 1330
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 3 cut(s) 359, 878, 1058
BspT104I TTCGAA 1 cut(s) 359
BsrBI CCGCTC 1 cut(s) 117
BsrDI GCAATG 2 cut(s) 324, 1050
BsrFI RCCGGY 1 cut(s) 514
BsrI ACTGG 1 cut(s) 213
BssAI RCCGGY 1 cut(s) 514
BssECI CCNNGG 6 cut(s) 55, 80, 178, 304, 590, 693
BssMI GATC 5 cut(s) 364, 883, 995, 1030, 1063
BssT1I CCWWGG 4 cut(s) 55, 80, 178, 693
Bst2UI CCWGG 2 cut(s) 162, 906
Bst4CI ACNGT 3 cut(s) 157, 1123, 1186
BstBAI YACGTR 1 cut(s) 1001
BstBI TTCGAA 1 cut(s) 359
BstC8I GCNNGC 5 cut(s) 103, 235, 510, 700, 874
BstDEI CTNAG 4 cut(s) 810, 982, 1140, 1212
BstDSI CCRYGG 2 cut(s) 304, 693
BstEII GGTNACC 1 cut(s) 781
BstF5I GGATG 2 cut(s) 75, 689
BstHHI GCGC 1 cut(s) 740
BstKTI GATC 5 cut(s) 367, 886, 998, 1033, 1066
BstMAI GTCTC 3 cut(s) 1190, 1208, 1254
BstMBI GATC 5 cut(s) 364, 883, 995, 1030, 1063
BstMWI GCNNNNNNNGC 3 cut(s) 455, 518, 869
BstNI CCWGG 2 cut(s) 162, 906
BstPI GGTNACC 1 cut(s) 781
BstSCI CCNGG 3 cut(s) 160, 589, 904
BstSFI CTRYAG 2 cut(s) 330, 1176
BstSLI GKGCMC 1 cut(s) 88
BsuRI GGCC 4 cut(s) 54, 86, 616, 876
BtgI CCRYGG 2 cut(s) 304, 693
BtsCI GGATG 2 cut(s) 75, 689
BtsIMutI CAGTG 2 cut(s) 206, 1191
Cac8I GCNNGC 5 cut(s) 103, 235, 510, 700, 874
CfoI GCGC 1 cut(s) 740
Cfr10I RCCGGY 1 cut(s) 514
Cfr13I GGNCC 5 cut(s) 52, 84, 85, 542, 1093
CsiI ACCWGGT 1 cut(s) 904
Csp6I GTAC 2 cut(s) 1144, 1317
CviAII CATG 3 cut(s) 475, 694, 707
CviQI GTAC 2 cut(s) 1144, 1317
DdeI CTNAG 4 cut(s) 810, 982, 1140, 1212
DpnI GATC 5 cut(s) 366, 885, 997, 1032, 1065
DpnII GATC 5 cut(s) 364, 883, 995, 1030, 1063
DrdI GACNNNNNNGTC 1 cut(s) 592
DseDI GACNNNNNNGTC 1 cut(s) 592
Eco130I CCWWGG 4 cut(s) 55, 80, 178, 693
Eco24I GRGCYC 2 cut(s) 88, 1333
Eco32I GATATC 2 cut(s) 301, 681
Eco47I GGWCC 2 cut(s) 542, 1093
Eco57I CTGAAG 2 cut(s) 12, 696
Eco72I CACGTG 1 cut(s) 1001
Eco91I GGTNACC 1 cut(s) 781
EcoO109I RGGNCCY 3 cut(s) 52, 85, 542
EcoO65I GGTNACC 1 cut(s) 781
EcoRI GAATTC 1 cut(s) 814
EcoRII CCWGG 2 cut(s) 160, 904
EcoRV GATATC 2 cut(s) 301, 681
EcoT14I CCWWGG 4 cut(s) 55, 80, 178, 693
EcoT22I ATGCAT 1 cut(s) 382
EcoT38I GRGCYC 2 cut(s) 88, 1333
ErhI CCWWGG 4 cut(s) 55, 80, 178, 693
FaeI CATG 3 cut(s) 478, 697, 710
FalI AAGNNNNNCTT 4 cut(s) 529, 561, 965, 997
FaqI GGGAC 1 cut(s) 514
FatI CATG 3 cut(s) 474, 693, 706
FauNDI CATATG 1 cut(s) 1219
FokI GGATG 2 cut(s) 62, 676
FriOI GRGCYC 2 cut(s) 88, 1333
FspBI CTAG 4 cut(s) 261, 860, 993, 1088
GlaI GCGC 1 cut(s) 739
HaeIII GGCC 4 cut(s) 54, 86, 616, 876
HapII CCGG 5 cut(s) 33, 515, 575, 590, 1193
HhaI GCGC 1 cut(s) 740
Hin1II CATG 3 cut(s) 478, 697, 710
Hin6I GCGC 1 cut(s) 738
HinP1I GCGC 1 cut(s) 738
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 1 cut(s) 820
HpaII CCGG 5 cut(s) 33, 515, 575, 590, 1193
HphI GGTGA 5 cut(s) 775, 845, 846, 1168, 1182
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 8 cut(s) 192, 298, 621, 813, 1030, 1035, 1107, 1213
Hpy188III TCNNGA 5 cut(s) 368, 537, 684, 824, 1326
Hpy8I GTNNAC 1 cut(s) 214
HpyAV CCTTC 3 cut(s) 380, 555, 1057
HpyCH4III ACNGT 3 cut(s) 157, 1123, 1186
HpyCH4IV ACGT 1 cut(s) 1000
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 518, 869
HpyF3I CTNAG 4 cut(s) 810, 982, 1140, 1212
HpySE526I ACGT 1 cut(s) 1000
Hsp92II CATG 3 cut(s) 478, 697, 710
HspAI GCGC 1 cut(s) 738
Kzo9I GATC 5 cut(s) 364, 883, 995, 1030, 1063
LmnI GCTCC 4 cut(s) 344, 869, 1168, 1328
LweI GCATC 3 cut(s) 84, 415, 910
MabI ACCWGGT 1 cut(s) 904
MaeI CTAG 4 cut(s) 261, 860, 993, 1088
MaeII ACGT 1 cut(s) 1000
MalI GATC 5 cut(s) 366, 885, 997, 1032, 1065
MbiI CCGCTC 1 cut(s) 117
MboI GATC 5 cut(s) 364, 883, 995, 1030, 1063
MboII GAAGA 4 cut(s) 18, 19, 525, 1315
MhlI GDGCHC 4 cut(s) 88, 898, 956, 1333
MluCI AATT 7 cut(s) 62, 639, 673, 814, 838, 961, 1100
MmeI TCCRAC 1 cut(s) 508
MnlI CCTC 9 cut(s) 328, 447, 533, 627, 714, 867, 1054, 1077, 1101
Mph1103I ATGCAT 1 cut(s) 382
MseI TTAA 2 cut(s) 134, 569
MspA1I CMGCKG 3 cut(s) 101, 632, 761
MspI CCGG 5 cut(s) 33, 515, 575, 590, 1193
MspR9I CCNGG 3 cut(s) 162, 591, 906
MvaI CCWGG 2 cut(s) 162, 906
MwoI GCNNNNNNNGC 3 cut(s) 455, 518, 869
NciI CCSGG 1 cut(s) 591
NcoI CCATGG 1 cut(s) 693
NdeI CATATG 1 cut(s) 1219
NdeII GATC 5 cut(s) 364, 883, 995, 1030, 1063
NlaIII CATG 3 cut(s) 478, 697, 710
NlaIV GGNNCC 3 cut(s) 86, 1094, 1330
NmuCI GTSAC 7 cut(s) 43, 665, 781, 824, 833, 1156, 1188
NsiI ATGCAT 1 cut(s) 382
NspV TTCGAA 1 cut(s) 359
PfeI GAWTC 1 cut(s) 820
PflFI GACNNNGTC 1 cut(s) 938
PfoI TCCNGGA 1 cut(s) 160
PmaCI CACGTG 1 cut(s) 1001
PmlI CACGTG 1 cut(s) 1001
Ppu21I YACGTR 1 cut(s) 1001
PpuMI RGGWCCY 1 cut(s) 542
Psp5II RGGWCCY 1 cut(s) 542
Psp6I CCWGG 2 cut(s) 160, 904
PspCI CACGTG 1 cut(s) 1001
PspEI GGTNACC 1 cut(s) 781
PspGI CCWGG 2 cut(s) 160, 904
PspN4I GGNNCC 3 cut(s) 86, 1094, 1330
PspOMI GGGCCC 1 cut(s) 84
PspPI GGNCC 5 cut(s) 52, 84, 85, 542, 1093
PspPPI RGGWCCY 1 cut(s) 542
PstI CTGCAG 1 cut(s) 334
PsyI GACNNNGTC 1 cut(s) 938
PvuII CAGCTG 3 cut(s) 101, 632, 761
RsaI GTAC 2 cut(s) 1145, 1318
RsaNI GTAC 2 cut(s) 1144, 1317
SaqAI TTAA 2 cut(s) 134, 569
Sau3AI GATC 5 cut(s) 364, 883, 995, 1030, 1063
Sau96I GGNCC 5 cut(s) 52, 84, 85, 542, 1093
ScrFI CCNGG 3 cut(s) 162, 591, 906
SduI GDGCHC 4 cut(s) 88, 898, 956, 1333
SexAI ACCWGGT 1 cut(s) 904
SfaNI GCATC 3 cut(s) 84, 415, 910
SfcI CTRYAG 2 cut(s) 330, 1176
SfuI TTCGAA 1 cut(s) 359
SgrAI CRCCGGYG 1 cut(s) 514
SinI GGWCC 2 cut(s) 542, 1093
Sse9I AATT 7 cut(s) 62, 639, 673, 814, 838, 961, 1100
SsiI CCGC 2 cut(s) 115, 518
SspMI CTAG 4 cut(s) 261, 860, 993, 1088
StyD4I CCNGG 3 cut(s) 160, 589, 904
StyI CCWWGG 4 cut(s) 55, 80, 178, 693
TaaI ACNGT 3 cut(s) 157, 1123, 1186
TaiI ACGT 1 cut(s) 1003
TaqI TCGA 2 cut(s) 359, 818
TasI AATT 7 cut(s) 62, 639, 673, 814, 838, 961, 1100
TatI WGTACW 2 cut(s) 1143, 1316
TauI GCSGC 1 cut(s) 521
TfiI GAWTC 1 cut(s) 820
Tru1I TTAA 2 cut(s) 134, 569
Tru9I TTAA 2 cut(s) 134, 569
TscAI CASTG 2 cut(s) 213, 1191
TseFI GTSAC 7 cut(s) 43, 665, 781, 824, 833, 1156, 1188
Tsp45I GTSAC 7 cut(s) 43, 665, 781, 824, 833, 1156, 1188
TspDTI ATGAA 5 cut(s) 12, 54, 484, 537, 1039
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 2 cut(s) 213, 1191
Tth111I GACNNNGTC 1 cut(s) 938
VpaK11BI GGWCC 2 cut(s) 542, 1093
XapI RAATTY 3 cut(s) 814, 961, 1100
XspI CTAG 4 cut(s) 261, 860, 993, 1088
Zsp2I ATGCAT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.