subtilisin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 349 |
| Acc36I | ACCTGC | 2 cut(s) | 330, 1772 |
| Acc65I | GGTACC | 1 cut(s) | 413 |
| AccB1I | GGYRCC | 3 cut(s) | 413, 736, 782 |
| AccI | GTMKAC | 1 cut(s) | 2051 |
| AccII | CGCG | 1 cut(s) | 1832 |
| AciI | CCGC | 2 cut(s) | 672, 729 |
| AcoI | YGGCCR | 7 cut(s) | 389, 446, 669, 687, 753, 1770, 2253 |
| AcsI | RAATTY | 1 cut(s) | 1577 |
| AcuI | CTGAAG | 2 cut(s) | 294, 2241 |
| AcvI | CACGTG | 1 cut(s) | 751 |
| AcyI | GRCGYC | 2 cut(s) | 737, 927 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 297, 444, 968, 1074, 1331, 1813, 2023, 2209, 2251 |
| AflIII | ACRYGT | 1 cut(s) | 2230 |
| AgsI | TTSAA | 8 cut(s) | 1037, 1110, 1153, 1232, 1394, 1582, 1972, 1986 |
| AhlI | ACTAGT | 1 cut(s) | 1433 |
| AjnI | CCWGG | 4 cut(s) | 295, 715, 1191, 1707 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 596, 628, 1367, 1399 |
| Alw21I | GWGCWC | 2 cut(s) | 1651, 1867 |
| Alw26I | GTCTC | 5 cut(s) | 79, 263, 312, 1408, 2177 |
| Alw44I | GTGCAC | 2 cut(s) | 1647, 1863 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 722 |
| Ama87I | CYCGRG | 2 cut(s) | 741, 1813 |
| ApaLI | GTGCAC | 2 cut(s) | 1647, 1863 |
| ApeKI | GCWGC | 6 cut(s) | 806, 1010, 1517, 1619, 1631, 1670 |
| ApoI | RAATTY | 1 cut(s) | 1577 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1548, 1580 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1534 |
| Asp718I | GGTACC | 1 cut(s) | 413 |
| AspLEI | GCGC | 3 cut(s) | 739, 877, 1636 |
| AspS9I | GGNCC | 4 cut(s) | 886, 959, 1451, 1804 |
| AsuC2I | CCSGG | 6 cut(s) | 439, 691, 1092, 1500, 1814, 1815 |
| AsuHPI | GGTGA | 7 cut(s) | 143, 607, 1346, 1754, 1891, 2038, 2248 |
| AvaI | CYCGRG | 2 cut(s) | 741, 1813 |
| AvaII | GGWCC | 3 cut(s) | 886, 959, 1451 |
| BaeGI | GKGCMC | 3 cut(s) | 1613, 1651, 1867 |
| BalI | TGGCCA | 4 cut(s) | 448, 755, 1772, 2255 |
| BanI | GGYRCC | 3 cut(s) | 413, 736, 782 |
| BanII | GRGCYC | 1 cut(s) | 748 |
| BbrPI | CACGTG | 1 cut(s) | 751 |
| BbsI | GAAGAC | 3 cut(s) | 922, 1098, 2176 |
| Bbv12I | GWGCWC | 2 cut(s) | 1651, 1867 |
| BbvI | GCAGC | 6 cut(s) | 793, 1022, 1504, 1618, 1631, 1682 |
| BccI | CCATC | 7 cut(s) | 24, 209, 492, 818, 860, 1462, 1904 |
| BceAI | ACGGC | 2 cut(s) | 421, 684 |
| BciT130I | CCWGG | 4 cut(s) | 297, 717, 1193, 1709 |
| BclI | TGATCA | 1 cut(s) | 148 |
| BcnI | CCSGG | 6 cut(s) | 439, 691, 1092, 1500, 1814, 1815 |
| BcoDI | GTCTC | 5 cut(s) | 79, 263, 312, 1408, 2177 |
| BcuI | ACTAGT | 1 cut(s) | 1433 |
| BfaI | CTAG | 5 cut(s) | 1014, 1434, 1446, 1761, 2296 |
| BfmI | CTRYAG | 1 cut(s) | 1668 |
| BfoI | RGCGCY | 1 cut(s) | 740 |
| BfuAI | ACCTGC | 2 cut(s) | 330, 1772 |
| BglII | AGATCT | 1 cut(s) | 1939 |
| BisI | GCNGC | 8 cut(s) | 672, 730, 807, 1011, 1518, 1620, 1632, 1671 |
| BlpI | GCTNAGC | 1 cut(s) | 2141 |
| BlsI | GCNGC | 8 cut(s) | 673, 731, 808, 1012, 1519, 1621, 1633, 1672 |
| Bme18I | GGWCC | 3 cut(s) | 886, 959, 1451 |
| BmeT110I | CYCGRG | 2 cut(s) | 741, 1813 |
| BmgT120I | GGNCC | 4 cut(s) | 886, 959, 1451, 1804 |
| BmiI | GGNNCC | 4 cut(s) | 415, 738, 784, 888 |
| BmrI | ACTGGG | 1 cut(s) | 349 |
| BmsI | GCATC | 2 cut(s) | 704, 1225 |
| BmuI | ACTGGG | 1 cut(s) | 349 |
| BpiI | GAAGAC | 3 cut(s) | 922, 1098, 2176 |
| BpmI | CTGGAG | 4 cut(s) | 61, 279, 296, 1277 |
| Bpu10I | CCTNAGC | 1 cut(s) | 270 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2141 |
| BpuEI | CTTGAG | 3 cut(s) | 1229, 1463, 1992 |
| BpuMI | CCSGG | 6 cut(s) | 439, 691, 1092, 1500, 1814, 1815 |
| BsaAI | YACGTR | 2 cut(s) | 751, 1849 |
| BsaHI | GRCGYC | 2 cut(s) | 737, 927 |
| BsaI | GGTCTC | 2 cut(s) | 312, 1408 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1053, 1083 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 297, 444, 968, 1074, 1331, 1813, 2023, 2209, 2251 |
| Bse118I | RCCGGY | 3 cut(s) | 325, 391, 1756 |
| Bse1I | ACTGG | 1 cut(s) | 355 |
| Bse3DI | GCAATG | 2 cut(s) | 1959, 2058 |
| BseBI | CCWGG | 4 cut(s) | 297, 717, 1193, 1709 |
| BseGI | GGATG | 8 cut(s) | 108, 844, 871, 1083, 1598, 1651, 1704, 2278 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 297, 444, 968, 1074, 1331, 1813, 2023, 2209, 2251 |
| BseMI | GCAATG | 2 cut(s) | 1959, 2058 |
| BseMII | CTCAG | 4 cut(s) | 261, 924, 2132, 2278 |
| BseNI | ACTGG | 1 cut(s) | 355 |
| BseSI | GKGCMC | 3 cut(s) | 1613, 1651, 1867 |
| BseX3I | CGGCCG | 1 cut(s) | 669 |
| BseXI | GCAGC | 6 cut(s) | 793, 1022, 1504, 1618, 1631, 1682 |
| BseYI | CCCAGC | 1 cut(s) | 1622 |
| BsgI | GTGCAG | 2 cut(s) | 1029, 1479 |
| Bsh1236I | CGCG | 1 cut(s) | 1832 |
| Bsh1285I | CGRYCG | 1 cut(s) | 672 |
| BshNI | GGYRCC | 3 cut(s) | 413, 736, 782 |
| BsiEI | CGRYCG | 1 cut(s) | 672 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1651, 1867 |
| BsiHKCI | CYCGRG | 2 cut(s) | 741, 1813 |
| BslFI | GGGAC | 6 cut(s) | 337, 647, 664, 788, 1609, 1830 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 297, 444, 968, 1074, 1331, 1813, 2023, 2209, 2251 |
| BsmAI | GTCTC | 5 cut(s) | 79, 263, 312, 1408, 2177 |
| BsmBI | CGTCTC | 1 cut(s) | 2177 |
| BsmFI | GGGAC | 6 cut(s) | 337, 647, 664, 788, 1609, 1830 |
| BsmI | GAATGC | 1 cut(s) | 1141 |
| Bso31I | GGTCTC | 2 cut(s) | 312, 1408 |
| BsoBI | CYCGRG | 2 cut(s) | 741, 1813 |
| Bsp1286I | GDGCHC | 4 cut(s) | 748, 1613, 1651, 1867 |
| Bsp1407I | TGTACA | 2 cut(s) | 175, 759 |
| Bsp143I | GATC | 4 cut(s) | 148, 1024, 1904, 1939 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2141 |
| Bsp19I | CCATGG | 3 cut(s) | 915, 1602, 1773 |
| BspACI | CCGC | 2 cut(s) | 672, 729 |
| BspCNI | CTCAG | 4 cut(s) | 262, 923, 2133, 2277 |
| BspFNI | CGCG | 1 cut(s) | 1832 |
| BspHI | TCATGA | 2 cut(s) | 88, 1131 |
| BspLI | GGNNCC | 4 cut(s) | 415, 738, 784, 888 |
| BspMAI | CTGCAG | 1 cut(s) | 1672 |
| BspMI | ACCTGC | 2 cut(s) | 330, 1772 |
| BspQI | GCTCTTC | 1 cut(s) | 541 |
| BspT107I | GGYRCC | 3 cut(s) | 413, 736, 782 |
| BspTNI | GGTCTC | 2 cut(s) | 312, 1408 |
| BsrDI | GCAATG | 2 cut(s) | 1959, 2058 |
| BsrFI | RCCGGY | 3 cut(s) | 325, 391, 1756 |
| BsrGI | TGTACA | 2 cut(s) | 175, 759 |
| BsrI | ACTGG | 1 cut(s) | 355 |
| BssAI | RCCGGY | 3 cut(s) | 325, 391, 1756 |
| BssMI | GATC | 4 cut(s) | 148, 1024, 1904, 1939 |
| BssNI | GRCGYC | 2 cut(s) | 737, 927 |
| BssT1I | CCWWGG | 5 cut(s) | 915, 992, 1602, 1773, 1878 |
| Bst2UI | CCWGG | 4 cut(s) | 297, 717, 1193, 1709 |
| Bst4CI | ACNGT | 9 cut(s) | 175, 644, 833, 1006, 1228, 1429, 1549, 2026, 2188 |
| Bst6I | CTCTTC | 3 cut(s) | 211, 541, 667 |
| BstACI | GRCGYC | 2 cut(s) | 737, 927 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1244 |
| BstAUI | TGTACA | 2 cut(s) | 175, 759 |
| BstBAI | YACGTR | 2 cut(s) | 751, 1849 |
| BstC8I | GCNNGC | 4 cut(s) | 727, 819, 1609, 2065 |
| BstDEI | CTNAG | 6 cut(s) | 210, 270, 910, 1721, 2141, 2264 |
| BstDSI | CCRYGG | 3 cut(s) | 915, 1602, 1773 |
| BstEII | GGTNACC | 2 cut(s) | 2026, 2198 |
| BstF5I | GGATG | 8 cut(s) | 108, 844, 871, 1083, 1598, 1651, 1704, 2278 |
| BstFNI | CGCG | 1 cut(s) | 1832 |
| BstH2I | RGCGCY | 1 cut(s) | 740 |
| BstHHI | GCGC | 3 cut(s) | 739, 877, 1636 |
| BstKTI | GATC | 4 cut(s) | 151, 1027, 1907, 1942 |
| BstMAI | GTCTC | 5 cut(s) | 79, 263, 312, 1408, 2177 |
| BstMBI | GATC | 4 cut(s) | 148, 1024, 1904, 1939 |
| BstMCI | CGRYCG | 1 cut(s) | 672 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 743, 752, 1244, 1628, 1778 |
| BstNI | CCWGG | 4 cut(s) | 297, 717, 1193, 1709 |
| BstNSI | RCATGY | 3 cut(s) | 1143, 1619, 2234 |
| BstPI | GGTNACC | 2 cut(s) | 2026, 2198 |
| BstSFI | CTRYAG | 1 cut(s) | 1668 |
| BstSLI | GKGCMC | 3 cut(s) | 1613, 1651, 1867 |
| BstSNI | TACGTA | 1 cut(s) | 1849 |
| BstUI | CGCG | 1 cut(s) | 1832 |
| BstV1I | GCAGC | 6 cut(s) | 793, 1022, 1504, 1618, 1631, 1682 |
| BstV2I | GAAGAC | 3 cut(s) | 922, 1098, 2176 |
| BstX2I | RGATCY | 1 cut(s) | 1939 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 362, 498, 733, 2113 |
| BstYI | RGATCY | 1 cut(s) | 1939 |
| BstZI | CGGCCG | 1 cut(s) | 669 |
| BtgI | CCRYGG | 3 cut(s) | 915, 1602, 1773 |
| BtsCI | GGATG | 8 cut(s) | 108, 844, 871, 1083, 1598, 1651, 1704, 2278 |
| BtsI | GCAGTG | 1 cut(s) | 828 |
| BtsIMutI | CAGTG | 4 cut(s) | 171, 256, 828, 2217 |
| BveI | ACCTGC | 2 cut(s) | 330, 1772 |
| Cac8I | GCNNGC | 4 cut(s) | 727, 819, 1609, 2065 |
| CaiI | CAGNNNCTG | 1 cut(s) | 722 |
| CciI | TCATGA | 2 cut(s) | 88, 1131 |
| CfoI | GCGC | 3 cut(s) | 739, 877, 1636 |
| Cfr10I | RCCGGY | 3 cut(s) | 325, 391, 1756 |
| Cfr13I | GGNCC | 4 cut(s) | 886, 959, 1451, 1804 |
| Cfr9I | CCCGGG | 1 cut(s) | 1813 |
| CseI | GACGC | 4 cut(s) | 479, 661, 916, 1838 |
| DdeI | CTNAG | 6 cut(s) | 210, 270, 910, 1721, 2141, 2264 |
| DinI | GGCGCC | 1 cut(s) | 738 |
| DpnI | GATC | 4 cut(s) | 150, 1026, 1906, 1941 |
| DpnII | GATC | 4 cut(s) | 148, 1024, 1904, 1939 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 349 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 349 |
| EaeI | YGGCCR | 7 cut(s) | 389, 446, 669, 687, 753, 1770, 2253 |
| EagI | CGGCCG | 1 cut(s) | 669 |
| Eam1104I | CTCTTC | 3 cut(s) | 211, 541, 667 |
| EarI | CTCTTC | 3 cut(s) | 211, 541, 667 |
| EclXI | CGGCCG | 1 cut(s) | 669 |
| Eco105I | TACGTA | 1 cut(s) | 1849 |
| Eco130I | CCWWGG | 5 cut(s) | 915, 992, 1602, 1773, 1878 |
| Eco147I | AGGCCT | 1 cut(s) | 281 |
| Eco24I | GRGCYC | 1 cut(s) | 748 |
| Eco31I | GGTCTC | 2 cut(s) | 312, 1408 |
| Eco47I | GGWCC | 3 cut(s) | 886, 959, 1451 |
| Eco52I | CGGCCG | 1 cut(s) | 669 |
| Eco57I | CTGAAG | 2 cut(s) | 294, 2241 |
| Eco72I | CACGTG | 1 cut(s) | 751 |
| Eco88I | CYCGRG | 2 cut(s) | 741, 1813 |
| Eco91I | GGTNACC | 2 cut(s) | 2026, 2198 |
| EcoO109I | RGGNCCY | 3 cut(s) | 886, 959, 1804 |
| EcoO65I | GGTNACC | 2 cut(s) | 2026, 2198 |
| EcoRI | GAATTC | 1 cut(s) | 1577 |
| EcoRII | CCWGG | 4 cut(s) | 295, 715, 1191, 1707 |
| EcoT14I | CCWWGG | 5 cut(s) | 915, 992, 1602, 1773, 1878 |
| EcoT22I | ATGCAT | 1 cut(s) | 1141 |
| EcoT38I | GRGCYC | 1 cut(s) | 748 |
| EgeI | GGCGCC | 1 cut(s) | 738 |
| EheI | GGCGCC | 1 cut(s) | 738 |
| ErhI | CCWWGG | 5 cut(s) | 915, 992, 1602, 1773, 1878 |
| Esp3I | CGTCTC | 1 cut(s) | 2177 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 914, 946, 2129, 2161 |
| FaqI | GGGAC | 6 cut(s) | 337, 647, 664, 788, 1609, 1830 |
| FbaI | TGATCA | 1 cut(s) | 148 |
| FblI | GTMKAC | 1 cut(s) | 2051 |
| Fnu4HI | GCNGC | 8 cut(s) | 672, 730, 807, 1011, 1518, 1620, 1632, 1671 |
| FokI | GGATG | 8 cut(s) | 95, 851, 878, 1090, 1585, 1638, 1691, 2285 |
| FriOI | GRGCYC | 1 cut(s) | 748 |
| Fsp4HI | GCNGC | 8 cut(s) | 672, 730, 807, 1011, 1518, 1620, 1632, 1671 |
| FspBI | CTAG | 5 cut(s) | 1014, 1434, 1446, 1761, 2296 |
| GlaI | GCGC | 3 cut(s) | 738, 876, 1635 |
| GluI | GCNGC | 8 cut(s) | 672, 730, 807, 1011, 1518, 1620, 1632, 1671 |
| GsaI | CCCAGC | 1 cut(s) | 1626 |
| GsuI | CTGGAG | 4 cut(s) | 61, 279, 296, 1277 |
| HaeII | RGCGCY | 1 cut(s) | 740 |
| HgaI | GACGC | 4 cut(s) | 479, 661, 916, 1838 |
| HhaI | GCGC | 3 cut(s) | 739, 877, 1636 |
| Hin1I | GRCGYC | 2 cut(s) | 737, 927 |
| Hin6I | GCGC | 3 cut(s) | 737, 875, 1634 |
| HinP1I | GCGC | 3 cut(s) | 737, 875, 1634 |
| HincII | GTYRAC | 3 cut(s) | 1552, 2052, 2228 |
| HindII | GTYRAC | 3 cut(s) | 1552, 2052, 2228 |
| HindIII | AAGCTT | 2 cut(s) | 460, 1381 |
| HpaI | GTTAAC | 1 cut(s) | 1552 |
| HphI | GGTGA | 7 cut(s) | 143, 607, 1346, 1754, 1891, 2038, 2248 |
| Hpy188I | TCNGA | 6 cut(s) | 484, 796, 860, 1702, 2018, 2272 |
| Hpy99I | CGWCG | 5 cut(s) | 473, 644, 1429, 1702, 1832 |
| HpyAV | CCTTC | 7 cut(s) | 916, 950, 1325, 1962, 2013, 2123, 2216 |
| HpyCH4III | ACNGT | 9 cut(s) | 175, 644, 833, 1006, 1228, 1429, 1549, 2026, 2188 |
| HpyCH4IV | ACGT | 5 cut(s) | 334, 750, 1221, 1697, 1848 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 743, 752, 1244, 1628, 1778 |
| HpyF3I | CTNAG | 6 cut(s) | 210, 270, 910, 1721, 2141, 2264 |
| HpySE526I | ACGT | 5 cut(s) | 334, 750, 1221, 1697, 1848 |
| Hsp92I | GRCGYC | 2 cut(s) | 737, 927 |
| HspAI | GCGC | 3 cut(s) | 737, 875, 1634 |
| KasI | GGCGCC | 1 cut(s) | 736 |
| KpnI | GGTACC | 1 cut(s) | 417 |
| Ksp22I | TGATCA | 1 cut(s) | 148 |
| KspAI | GTTAAC | 1 cut(s) | 1552 |
| Kzo9I | GATC | 4 cut(s) | 148, 1024, 1904, 1939 |
| LguI | GCTCTTC | 1 cut(s) | 541 |
| LmnI | GCTCC | 2 cut(s) | 383, 1778 |
| Lsp1109I | GCAGC | 6 cut(s) | 793, 1022, 1504, 1618, 1631, 1682 |
| LweI | GCATC | 2 cut(s) | 704, 1225 |
| MaeI | CTAG | 5 cut(s) | 1014, 1434, 1446, 1761, 2296 |
| MaeII | ACGT | 5 cut(s) | 334, 750, 1221, 1697, 1848 |
| MalI | GATC | 4 cut(s) | 150, 1026, 1906, 1941 |
| MboI | GATC | 4 cut(s) | 148, 1024, 1904, 1939 |
| MfeI | CAATTG | 2 cut(s) | 1122, 1918 |
| MflI | RGATCY | 1 cut(s) | 1939 |
| MhlI | GDGCHC | 4 cut(s) | 748, 1613, 1651, 1867 |
| MlsI | TGGCCA | 4 cut(s) | 448, 755, 1772, 2255 |
| MluNI | TGGCCA | 4 cut(s) | 448, 755, 1772, 2255 |
| Mly113I | GGCGCC | 1 cut(s) | 737 |
| MlyI | GAGTC | 4 cut(s) | 1075, 1672, 1812, 2106 |
| MmeI | TCCRAC | 3 cut(s) | 120, 1680, 2074 |
| Mox20I | TGGCCA | 4 cut(s) | 448, 755, 1772, 2255 |
| Mph1103I | ATGCAT | 1 cut(s) | 1141 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1534 |
| MscI | TGGCCA | 4 cut(s) | 448, 755, 1772, 2255 |
| MslI | CAYNNNNRTG | 4 cut(s) | 113, 496, 1076, 1538 |
| Msp20I | TGGCCA | 4 cut(s) | 448, 755, 1772, 2255 |
| MspA1I | CMGCKG | 4 cut(s) | 674, 729, 1622, 2075 |
| MunI | CAATTG | 2 cut(s) | 1122, 1918 |
| Mva1269I | GAATGC | 1 cut(s) | 1141 |
| MvaI | CCWGG | 4 cut(s) | 297, 717, 1193, 1709 |
| MvnI | CGCG | 1 cut(s) | 1832 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 743, 752, 1244, 1628, 1778 |
| NarI | GGCGCC | 1 cut(s) | 737 |
| NciI | CCSGG | 6 cut(s) | 439, 691, 1092, 1500, 1814, 1815 |
| NcoI | CCATGG | 3 cut(s) | 915, 1602, 1773 |
| NdeII | GATC | 4 cut(s) | 148, 1024, 1904, 1939 |
| NlaIV | GGNNCC | 4 cut(s) | 415, 738, 784, 888 |
| NmuCI | GTSAC | 9 cut(s) | 49, 335, 596, 607, 1028, 1069, 1142, 2026, 2068 |
| NsiI | ATGCAT | 1 cut(s) | 1141 |
| NspI | RCATGY | 3 cut(s) | 1143, 1619, 2234 |
| PagI | TCATGA | 2 cut(s) | 88, 1131 |
| PceI | AGGCCT | 1 cut(s) | 281 |
| PciI | ACATGT | 1 cut(s) | 2230 |
| PciSI | GCTCTTC | 1 cut(s) | 541 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 347 |
| PctI | GAATGC | 1 cut(s) | 1141 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1534 |
| PfeI | GAWTC | 6 cut(s) | 92, 119, 585, 987, 1064, 1982 |
| PflFI | GACNNNGTC | 1 cut(s) | 1696 |
| PfoI | TCCNGGA | 1 cut(s) | 1707 |
| PkrI | GCNGC | 8 cut(s) | 673, 731, 808, 1012, 1519, 1621, 1633, 1672 |
| PleI | GAGTC | 4 cut(s) | 1075, 1671, 1812, 2105 |
| PluTI | GGCGCC | 1 cut(s) | 740 |
| PmaCI | CACGTG | 1 cut(s) | 751 |
| PmlI | CACGTG | 1 cut(s) | 751 |
| PpsI | GAGTC | 4 cut(s) | 1075, 1671, 1812, 2105 |
| Ppu21I | YACGTR | 2 cut(s) | 751, 1849 |
| PpuMI | RGGWCCY | 2 cut(s) | 886, 959 |
| PscI | ACATGT | 1 cut(s) | 2230 |
| Psp5II | RGGWCCY | 2 cut(s) | 886, 959 |
| Psp6I | CCWGG | 4 cut(s) | 295, 715, 1191, 1707 |
| PspCI | CACGTG | 1 cut(s) | 751 |
| PspEI | GGTNACC | 2 cut(s) | 2026, 2198 |
| PspFI | CCCAGC | 1 cut(s) | 1622 |
| PspGI | CCWGG | 4 cut(s) | 295, 715, 1191, 1707 |
| PspN4I | GGNNCC | 4 cut(s) | 415, 738, 784, 888 |
| PspPI | GGNCC | 4 cut(s) | 886, 959, 1451, 1804 |
| PspPPI | RGGWCCY | 2 cut(s) | 886, 959 |
| PstI | CTGCAG | 1 cut(s) | 1672 |
| PstNI | CAGNNNCTG | 1 cut(s) | 722 |
| PsuI | RGATCY | 1 cut(s) | 1939 |
| PsyI | GACNNNGTC | 1 cut(s) | 1696 |
| PvuII | CAGCTG | 2 cut(s) | 1622, 2075 |
| RseI | CAYNNNNRTG | 4 cut(s) | 113, 496, 1076, 1538 |
| SalI | GTCGAC | 1 cut(s) | 2050 |
| SapI | GCTCTTC | 1 cut(s) | 541 |
| SatI | GCNGC | 8 cut(s) | 672, 730, 807, 1011, 1518, 1620, 1632, 1671 |
| Sau3AI | GATC | 4 cut(s) | 148, 1024, 1904, 1939 |
| Sau96I | GGNCC | 4 cut(s) | 886, 959, 1451, 1804 |
| SchI | GAGTC | 4 cut(s) | 1075, 1672, 1812, 2106 |
| SduI | GDGCHC | 4 cut(s) | 748, 1613, 1651, 1867 |
| SfaNI | GCATC | 2 cut(s) | 704, 1225 |
| SfcI | CTRYAG | 1 cut(s) | 1668 |
| SfoI | GGCGCC | 1 cut(s) | 738 |
| SinI | GGWCC | 3 cut(s) | 886, 959, 1451 |
| SmaI | CCCGGG | 1 cut(s) | 1815 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 113, 496, 1076, 1538 |
| SmlI | CTYRAG | 3 cut(s) | 1208, 1478, 2007 |
| SmoI | CTYRAG | 3 cut(s) | 1208, 1478, 2007 |
| SnaBI | TACGTA | 1 cut(s) | 1849 |
| SpeI | ACTAGT | 1 cut(s) | 1433 |
| SseBI | AGGCCT | 1 cut(s) | 281 |
| SsiI | CCGC | 2 cut(s) | 672, 729 |
| SspDI | GGCGCC | 1 cut(s) | 736 |
| SspMI | CTAG | 5 cut(s) | 1014, 1434, 1446, 1761, 2296 |
| StuI | AGGCCT | 1 cut(s) | 281 |
| StyI | CCWWGG | 5 cut(s) | 915, 992, 1602, 1773, 1878 |
| TaaI | ACNGT | 9 cut(s) | 175, 644, 833, 1006, 1228, 1429, 1549, 2026, 2188 |
| TaiI | ACGT | 5 cut(s) | 337, 753, 1224, 1700, 1851 |
| TaqI | TCGA | 6 cut(s) | 639, 813, 1528, 1913, 2051, 2167 |
| TatI | WGTACW | 5 cut(s) | 30, 175, 184, 759, 1996 |
| TauI | GCSGC | 2 cut(s) | 674, 732 |
| TfiI | GAWTC | 6 cut(s) | 92, 119, 585, 987, 1064, 1982 |
| TscAI | CASTG | 4 cut(s) | 178, 256, 828, 2224 |
| TseFI | GTSAC | 9 cut(s) | 49, 335, 596, 607, 1028, 1069, 1142, 2026, 2068 |
| TseI | GCWGC | 6 cut(s) | 806, 1010, 1517, 1619, 1631, 1670 |
| Tsp45I | GTSAC | 9 cut(s) | 49, 335, 596, 607, 1028, 1069, 1142, 2026, 2068 |
| TspGWI | ACGGA | 2 cut(s) | 1057, 1920 |
| TspMI | CCCGGG | 1 cut(s) | 1813 |
| TspRI | CASTG | 4 cut(s) | 178, 256, 828, 2224 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1696 |
| VneI | GTGCAC | 2 cut(s) | 1647, 1863 |
| VpaK11BI | GGWCC | 3 cut(s) | 886, 959, 1451 |
| XapI | RAATTY | 1 cut(s) | 1577 |
| XbaI | TCTAGA | 1 cut(s) | 1445 |
| XceI | RCATGY | 3 cut(s) | 1143, 1619, 2234 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 424 |
| XmaI | CCCGGG | 1 cut(s) | 1813 |
| XmiI | GTMKAC | 1 cut(s) | 2051 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1534 |
| XspI | CTAG | 5 cut(s) | 1014, 1434, 1446, 1761, 2296 |
| Zsp2I | ATGCAT | 1 cut(s) | 1141 |