pycom08g07990

subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001268
Physical Location & Seq
Forward (+)
6434320 .. 6436822
2503 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g07990.3

Sequence Viewer

Length: 2376 bp
ATGGTGATGAGGAATATATCTATATATTTCATTGCTTCCCCCATTTGTATTCTAATTTTTTCGCCTCCTCCCTTGTGCTCAACATCAATGTCTAGTTACTACCACGTTTTGTATTTCTGGCTTTCCATTACCACCATCTCACACCTTGCAATTGTCTCAACCTTTGCTCAATCCCAAAACTATATCATCCACATGGATTCATCAATGATGCCTAAAGCCTTTTCTGATCATCATTCATGGTACGTGGCAACATTACACTCCACATTAGGCAAGTTTACGCCTATCACCACCGGGACCTCCTCCTCCTCTGCCCTCTCTTCCAAACTCATTTATAGTTATACTCATGTCATGAATGGTTTCAGTGCGAGTCTCACTGCTTCCGAGCTTGAGGCCTTAAAAAGCTCTCTAGGCTACATTTCTTCAGTGAAAGACTTGCCAATAATAAAAGACACAACTCACTCCTCTCAATTTCTTGGCCTCAACTCCAGATTCGGAGCGTGGCCGGTTTCGAATTACGGCAAAGATGTGATCATTGGGTTGGTGGACACGGGTGTTTGGCCAGAAAGTGAAAGTTTTAACGAAGATGGGATGTCAGAAATCCCACCAAGATGGAAAGGAGAATGTGAAAGTGGCACCCAATTCAATTCCTCATTGTGCAACAAAAAGTTGATTGGGGCAAAGTTCTTTAACAAAGGGCTCATTGCCCAAAATCCCAATCTGACCATTGGAGTGAATTCAACAAGGGATACTGAAGGGCATGGTACCCACACCTCGTCTACTGCCGCAGGAAATTACGTGGCAGGGGCATCTTTTTTTGGTTACGCCCCAGGAGTTGCCAGCGGCATGGCTCCTAGGGCGCATGTGGCCATGTACAAGGCGCTTTGGGATGAGGGAGCATTGTCGTCGGATATAATTGCTGCTATTGAGCAAGCAATTATAGACGGTGTGGATGTTTTGTCTTTGTCATTTGGGTTAGATGGTGTTGCTTTGTATGAGGATCCTGTTGCCATAGCAACATTTTCTGCCTTGGAGAGAGGTGTTTTTGTGTCCACGTCTGCAGGAAATGAAGGACCTTTCTTTGCTACCTTGCACAATGGTATCCCTTGGGTTCTCACGGTTGCAGCTGGTACAATTGACCGGGAATTCGAAGGAACTGAGCATCTTGGAAATGGAAACTCTGTCACGGGGTCTACTCTCTATCCCGGAAATTCATCTTCAACCCAATTTCCGGTTGTTTTCTTTGATGCATCGATCGTGTGCCAGGACAAAAATGATACGCTGAACGATCAAGTTTATAATGTACGCAGCGCAAATGTTGCCGGAGGTATCTTCATTACCAACAACACAGATTTGGAGTTGTTCATCCAAAGCTCTTATCCCGCAATCTTTCTTAGTCCAAAGGAAGGTGAAGTGATCAAAGGTTACATCAAGAGCAACTCACAGCCAAAAGCAAGCTTGGAGTTTCAAAAAACACTTCTTGGTGCTAAACCAGCACCGAGTGTTACTAGCTACTCGTCTAGAGGGCCGTCCCCTAGCTTCCCGTGGACCCTGAAGCCAGACATTGTGGCTCCAGGGTCGTTAGTCCTGGCTGCATGGCCTCAAAATACCTCCGCAGCCGTGGTGGGCAAGAAAGACTTGTTTAGTAATTTTAATCTACTGTCAGGGACATCCATGTCGTGCCCCCATGCAGCTGGAATAGCTGCGCTTCTGAAAGGTGCGCACCCCGAATGGAGCCCTGCTGCTATTAGATCCGCCATGATGACGACATCAGACATATTGGATAACACCGGTAGCCCCATAAAAGACATTGGCAACGCTAACCAACCAGCTAGTCCTCTAGCCATCGGAGCCGGGCATGTCAACCCTAACAAGGCCCTAGACCCCGGGCTCCTTTACGACGTGAATATTGACGACTACGTTAACCTTCTTTGTGCACTCAACTACACCAACAAGCAAATCCAAACCATCACTAAATCAGCCTCGAACAACTGTTCAACTCCTTCCTTAGATCTTAATTACCCCTCTTTCATAGCATTCTTCAATTCAAATGACTCGAATTCGAACGTACAATCTACTCATGAATTCCGGAGGACGGTGACCAACATAGGAAAGGGACTGTCGACCTACGTTGCGAGCGTGACACCATTGAAAGGGTTTGTGGTTGGAGTCGTTCCGAAGAAGTTGGAGTTCGAGGAGCAGGGTGAGAAGCTGAGCTTTGTGCTAAGTATAAAAGGACCAAGAGTAATGAAAGAGGCAGTGGTTTTTGGTTATCTGAGTTGGGTGGAGAGTGGTGGTCAACATGTGGTGAGAAGCCCCGTCGTGCTTGCCTGTTCTAGCAAGTATGTGATCATTCCCTTGCTAAATAAAAAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

792

Amino Acids

84.69

Weight (kDa)

6.14

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1296
AasI GACNNNNNNGTC 1 cut(s) 1672
Acc16I TGCGCA 1 cut(s) 1719
Acc65I GGTACC 1 cut(s) 761
AccB1I GGYRCC 2 cut(s) 632, 761
AccI GTMKAC 3 cut(s) 776, 1190, 2120
AccIII TCCGGA 1 cut(s) 2085
AciI CCGC 5 cut(s) 783, 840, 1380, 1611, 1752
AclWI GGATC 3 cut(s) 992, 1005, 1743
AcoI YGGCCR 3 cut(s) 500, 557, 864
AcsI RAATTY 5 cut(s) 733, 1142, 1207, 2056, 2081
AcuI CTGAAG 3 cut(s) 405, 771, 1571
AdeI CACNNNGTG 1 cut(s) 1499
AfaI GTAC 6 cut(s) 242, 763, 872, 1129, 1302, 2067
AfiI CCNNNNNNNGG 5 cut(s) 1228, 1403, 1870, 2092, 2150
AflIII ACRYGT 1 cut(s) 2299
AgeI ACCGGT 1 cut(s) 1787
AgsI TTSAA 8 cut(s) 643, 738, 1218, 1466, 1995, 2041, 2046, 2149
AjiI CACGTC 2 cut(s) 1053, 1900
AjnI CCWGG 4 cut(s) 826, 1260, 1570, 1584
Alw21I GWGCWC 2 cut(s) 80, 1936
Alw26I GTCTC 2 cut(s) 160, 374
Alw44I GTGCAC 1 cut(s) 1932
AlwI GGATC 3 cut(s) 992, 1005, 1743
Ama87I CYCGRG 1 cut(s) 1883
Aor13HI TCCGGA 1 cut(s) 2085
AoxI GGCC 8 cut(s) 390, 475, 500, 557, 864, 1523, 1595, 1872
ApaLI GTGCAC 1 cut(s) 1932
ApeKI GCWGC 8 cut(s) 917, 1121, 1305, 1589, 1613, 1688, 1700, 1739
ApoI RAATTY 5 cut(s) 733, 1142, 1207, 2056, 2081
ArsI GACNNNNNNTTYG 2 cut(s) 1127, 1159
AsiGI ACCGGT 1 cut(s) 1787
Asp700I GAANNNNTTC 1 cut(s) 356
Asp718I GGTACC 1 cut(s) 761
AspA2I CCTAGG 1 cut(s) 851
AspLEI GCGC 5 cut(s) 859, 880, 1310, 1705, 1720
AspS9I GGNCC 6 cut(s) 294, 1070, 1523, 1545, 1873, 2234
AsuC2I CCSGG 6 cut(s) 292, 1139, 1203, 1852, 1884, 1885
AsuHPI GGTGA 6 cut(s) 16, 277, 1418, 2107, 2213, 2317
AsuII TTCGAA 3 cut(s) 509, 1146, 2060
AvaI CYCGRG 1 cut(s) 1883
AvaII GGWCC 4 cut(s) 294, 1070, 1545, 2234
AvrII CCTAGG 1 cut(s) 851
BaeGI GKGCMC 2 cut(s) 1682, 1936
BaeI ACNNNNGTAYC 2 cut(s) 1081, 1114
BalI TGGCCA 2 cut(s) 559, 866
BamHI GGATCC 1 cut(s) 997
BanI GGYRCC 2 cut(s) 632, 761
BanII GRGCYC 3 cut(s) 699, 1736, 1890
Bbv12I GWGCWC 2 cut(s) 80, 1936
BbvI GCAGC 8 cut(s) 904, 1133, 1317, 1576, 1625, 1687, 1700, 1726
BccI CCATC 6 cut(s) 143, 578, 603, 971, 1850, 1973
BceAI ACGGC 3 cut(s) 532, 1510, 1601
BcgI CGANNNNNNTGC 2 cut(s) 885, 919
BciT130I CCWGG 4 cut(s) 828, 1262, 1572, 1586
BciVI GTATCC 2 cut(s) 739, 1109
BclI TGATCA 4 cut(s) 226, 528, 1413, 2346
BcnI CCSGG 6 cut(s) 292, 1139, 1203, 1852, 1884, 1885
BcoDI GTCTC 2 cut(s) 160, 374
BfmI CTRYAG 1 cut(s) 1056
BfoI RGCGCY 1 cut(s) 881
BfuI GTATCC 2 cut(s) 739, 1109
BglII AGATCT 1 cut(s) 2008
BlnI CCTAGG 1 cut(s) 851
BlpI GCTNAGC 1 cut(s) 2210
Bme18I GGWCC 4 cut(s) 294, 1070, 1545, 2234
BmeT110I CYCGRG 1 cut(s) 1883
BmgBI CACGTC 2 cut(s) 1053, 1900
BmgT120I GGNCC 6 cut(s) 294, 1070, 1523, 1545, 1873, 2234
BmsI GCATC 5 cut(s) 198, 815, 1168, 1234, 1256
BpmI CTGGAG 2 cut(s) 469, 1554
Bpu1102I GCTNAGC 1 cut(s) 2210
Bpu14I TTCGAA 3 cut(s) 509, 1146, 2060
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 6 cut(s) 292, 1139, 1203, 1852, 1884, 1885
Bsa29I ATCGAT 1 cut(s) 1250
BsaAI YACGTR 2 cut(s) 244, 796
BsaJI CCNNGG 9 cut(s) 826, 851, 1026, 1103, 1541, 1571, 1617, 1882, 1883
BsaWI WCCGGW 3 cut(s) 1228, 1787, 2085
Bsc4I CCNNNNNNNGG 5 cut(s) 1228, 1403, 1870, 2092, 2150
Bse118I RCCGGY 2 cut(s) 502, 1787
Bse3DI GCAATG 2 cut(s) 30, 699
BseAI TCCGGA 1 cut(s) 2085
BseBI CCWGG 4 cut(s) 828, 1262, 1572, 1586
BseCI ATCGAT 1 cut(s) 1250
BseDI CCNNGG 9 cut(s) 826, 851, 1026, 1103, 1541, 1571, 1617, 1882, 1883
BseGI GGATG 6 cut(s) 186, 594, 892, 955, 1362, 1667
BseLI CCNNNNNNNGG 5 cut(s) 1228, 1403, 1870, 2092, 2150
BseMI GCAATG 2 cut(s) 30, 699
BseMII CTCAG 3 cut(s) 1146, 2201, 2264
BseRI GAGGAG 6 cut(s) 57, 289, 292, 295, 451, 2207
BseSI GKGCMC 2 cut(s) 1682, 1936
BseXI GCAGC 8 cut(s) 904, 1133, 1317, 1576, 1625, 1687, 1700, 1726
Bsh1285I CGRYCG 1 cut(s) 1254
BshFI GGCC 8 cut(s) 392, 477, 502, 559, 866, 1525, 1597, 1874
BshNI GGYRCC 2 cut(s) 632, 761
BshTI ACCGGT 1 cut(s) 1787
BshVI ATCGAT 1 cut(s) 1250
BsiEI CGRYCG 1 cut(s) 1254
BsiHKAI GWGCWC 2 cut(s) 80, 1936
BsiHKCI CYCGRG 1 cut(s) 1883
BslFI GGGAC 4 cut(s) 307, 1513, 1678, 2127
BslI CCNNNNNNNGG 5 cut(s) 1228, 1403, 1870, 2092, 2150
BsmAI GTCTC 2 cut(s) 160, 374
BsmFI GGGAC 4 cut(s) 307, 1513, 1678, 2127
BsmI GAATGC 1 cut(s) 2033
BsnI GGCC 8 cut(s) 392, 477, 502, 559, 866, 1525, 1597, 1874
BsoBI CYCGRG 1 cut(s) 1883
Bsp119I TTCGAA 3 cut(s) 509, 1146, 2060
Bsp1286I GDGCHC 6 cut(s) 80, 699, 1682, 1736, 1890, 1936
Bsp13I TCCGGA 1 cut(s) 2085
Bsp1407I TGTACA 1 cut(s) 870
Bsp143I GATC 9 cut(s) 226, 528, 997, 1251, 1285, 1413, 1748, 2008, 2346
Bsp1720I GCTNAGC 1 cut(s) 2210
BspACI CCGC 5 cut(s) 783, 840, 1380, 1611, 1752
BspANI GGCC 8 cut(s) 392, 477, 502, 559, 866, 1525, 1597, 1874
BspCNI CTCAG 3 cut(s) 1147, 2202, 2265
BspDI ATCGAT 1 cut(s) 1250
BspEI TCCGGA 1 cut(s) 2085
BspHI TCATGA 2 cut(s) 348, 2077
BspMAI CTGCAG 1 cut(s) 1060
BspPI GGATC 3 cut(s) 992, 1005, 1743
BspT104I TTCGAA 3 cut(s) 509, 1146, 2060
BspT107I GGYRCC 2 cut(s) 632, 761
BsrDI GCAATG 2 cut(s) 30, 699
BsrFI RCCGGY 2 cut(s) 502, 1787
BsrGI TGTACA 1 cut(s) 870
BssAI RCCGGY 2 cut(s) 502, 1787
BssECI CCNNGG 9 cut(s) 826, 851, 1026, 1103, 1541, 1571, 1617, 1882, 1883
BssMI GATC 9 cut(s) 226, 528, 997, 1251, 1285, 1413, 1748, 2008, 2346
BssT1I CCWWGG 3 cut(s) 851, 1026, 1103
Bst2UI CCWGG 4 cut(s) 828, 1262, 1572, 1586
Bst4CI ACNGT 6 cut(s) 944, 1117, 1659, 1991, 2095, 2118
Bst6I CTCTTC 1 cut(s) 322
BstAPI GCANNNNNTGC 1 cut(s) 1316
BstAUI TGTACA 1 cut(s) 870
BstBAI YACGTR 2 cut(s) 244, 796
BstBI TTCGAA 3 cut(s) 509, 1146, 2060
BstC8I GCNNGC 5 cut(s) 838, 930, 1453, 2134, 2325
BstDEI CTNAG 6 cut(s) 1155, 1391, 2005, 2210, 2222, 2273
BstDSI CCRYGG 2 cut(s) 1541, 1617
BstEII GGTNACC 1 cut(s) 2095
BstF5I GGATG 6 cut(s) 186, 594, 892, 955, 1362, 1667
BstH2I RGCGCY 1 cut(s) 881
BstHHI GCGC 5 cut(s) 859, 880, 1310, 1705, 1720
BstKTI GATC 9 cut(s) 229, 531, 1000, 1254, 1288, 1416, 1751, 2011, 2349
BstMAI GTCTC 2 cut(s) 160, 374
BstMBI GATC 9 cut(s) 226, 528, 997, 1251, 1285, 1413, 1748, 2008, 2346
BstMCI CGRYCG 1 cut(s) 1254
BstMWI GCNNNNNNNGC 7 cut(s) 408, 854, 863, 1316, 1490, 1697, 1847
BstNI CCWGG 4 cut(s) 828, 1262, 1572, 1586
BstNSI RCATGY 3 cut(s) 863, 1859, 2303
BstPI GGTNACC 1 cut(s) 2095
BstSFI CTRYAG 1 cut(s) 1056
BstSLI GKGCMC 2 cut(s) 1682, 1936
BstV1I GCAGC 8 cut(s) 904, 1133, 1317, 1576, 1625, 1687, 1700, 1726
BstX2I RGATCY 3 cut(s) 997, 1748, 2008
BstXI CCANNNNNNTGG 3 cut(s) 609, 844, 1691
BstYI RGATCY 3 cut(s) 997, 1748, 2008
Bsu15I ATCGAT 1 cut(s) 1250
BsuI GTATCC 2 cut(s) 739, 1109
BsuRI GGCC 8 cut(s) 392, 477, 502, 559, 866, 1525, 1597, 1874
BsuTUI ATCGAT 1 cut(s) 1250
BtgI CCRYGG 2 cut(s) 1541, 1617
BtrI CACGTC 2 cut(s) 1053, 1900
BtsCI GGATG 6 cut(s) 186, 594, 892, 955, 1362, 1667
BtsI GCAGTG 2 cut(s) 372, 2262
BtsIMutI CAGTG 4 cut(s) 367, 372, 429, 2262
Cac8I GCNNGC 5 cut(s) 838, 930, 1453, 2134, 2325
CciI TCATGA 2 cut(s) 348, 2077
CfoI GCGC 5 cut(s) 859, 880, 1310, 1705, 1720
Cfr10I RCCGGY 2 cut(s) 502, 1787
Cfr13I GGNCC 6 cut(s) 294, 1070, 1523, 1545, 1873, 2234
Cfr9I CCCGGG 1 cut(s) 1883
ClaI ATCGAT 1 cut(s) 1250
Csp6I GTAC 6 cut(s) 241, 762, 871, 1128, 1301, 2066
CspAI ACCGGT 1 cut(s) 1787
CviQI GTAC 6 cut(s) 241, 762, 871, 1128, 1301, 2066
DdeI CTNAG 6 cut(s) 1155, 1391, 2005, 2210, 2222, 2273
DpnI GATC 9 cut(s) 228, 530, 999, 1253, 1287, 1415, 1750, 2010, 2348
DpnII GATC 9 cut(s) 226, 528, 997, 1251, 1285, 1413, 1748, 2008, 2346
DraIII CACNNNGTG 1 cut(s) 1499
DrdI GACNNNNNNGTC 1 cut(s) 1672
DseDI GACNNNNNNGTC 1 cut(s) 1672
EaeI YGGCCR 3 cut(s) 500, 557, 864
Eam1104I CTCTTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 322
EciI GGCGGA 1 cut(s) 1741
Eco130I CCWWGG 3 cut(s) 851, 1026, 1103
Eco147I AGGCCT 1 cut(s) 392
Eco24I GRGCYC 3 cut(s) 699, 1736, 1890
Eco47I GGWCC 4 cut(s) 294, 1070, 1545, 2234
Eco57I CTGAAG 3 cut(s) 405, 771, 1571
Eco88I CYCGRG 1 cut(s) 1883
Eco91I GGTNACC 1 cut(s) 2095
EcoO109I RGGNCCY 3 cut(s) 294, 1070, 1873
EcoO65I GGTNACC 1 cut(s) 2095
EcoRI GAATTC 4 cut(s) 733, 1142, 2056, 2081
EcoRII CCWGG 4 cut(s) 826, 1260, 1570, 1584
EcoT14I CCWWGG 3 cut(s) 851, 1026, 1103
EcoT22I ATGCAT 1 cut(s) 1249
EcoT38I GRGCYC 3 cut(s) 699, 1736, 1890
ErhI CCWWGG 3 cut(s) 851, 1026, 1103
FalI AAGNNNNNCTT 4 cut(s) 1619, 1651, 2198, 2230
FaqI GGGAC 4 cut(s) 307, 1513, 1678, 2127
FauI CCCGC 1 cut(s) 1387
FbaI TGATCA 4 cut(s) 226, 528, 1413, 2346
FblI GTMKAC 3 cut(s) 776, 1190, 2120
FokI GGATG 6 cut(s) 173, 601, 899, 962, 1349, 1654
FriOI GRGCYC 3 cut(s) 699, 1736, 1890
FspAI RTGCGCAY 1 cut(s) 1719
FspI TGCGCA 1 cut(s) 1719
GlaI GCGC 5 cut(s) 858, 879, 1309, 1704, 1719
GsuI CTGGAG 2 cut(s) 469, 1554
HaeII RGCGCY 1 cut(s) 881
HaeIII GGCC 8 cut(s) 392, 477, 502, 559, 866, 1525, 1597, 1874
HhaI GCGC 5 cut(s) 859, 880, 1310, 1705, 1720
Hin6I GCGC 5 cut(s) 857, 878, 1308, 1703, 1718
HinP1I GCGC 5 cut(s) 857, 878, 1308, 1703, 1718
HincII GTYRAC 4 cut(s) 1861, 1921, 2121, 2297
HindII GTYRAC 4 cut(s) 1861, 1921, 2121, 2297
HindIII AAGCTT 1 cut(s) 1453
HinfI GANTC 5 cut(s) 197, 367, 489, 2051, 2166
HpaI GTTAAC 1 cut(s) 1921
HphI GGTGA 6 cut(s) 16, 277, 1418, 2107, 2213, 2317
Hpy188III TCNNGA 6 cut(s) 349, 486, 1429, 1518, 2078, 2086
Hpy99I CGWCG 3 cut(s) 907, 1901, 2321
HpyAV CCTTC 6 cut(s) 746, 1061, 1142, 1397, 1934, 2010
HpyCH4III ACNGT 6 cut(s) 944, 1117, 1659, 1991, 2095, 2118
HpyCH4IV ACGT 8 cut(s) 105, 243, 795, 1052, 1899, 1917, 2064, 2127
HpyCH4V TGCA 9 cut(s) 149, 657, 1058, 1090, 1121, 1247, 1592, 1688, 1934
HpyF10VI GCNNNNNNNGC 7 cut(s) 408, 854, 863, 1316, 1490, 1697, 1847
HpyF3I CTNAG 6 cut(s) 1155, 1391, 2005, 2210, 2222, 2273
HpySE526I ACGT 8 cut(s) 105, 243, 795, 1052, 1899, 1917, 2064, 2127
HspAI GCGC 5 cut(s) 857, 878, 1308, 1703, 1718
Kpn2I TCCGGA 1 cut(s) 2085
KpnI GGTACC 1 cut(s) 765
Ksp22I TGATCA 4 cut(s) 226, 528, 1413, 2346
KspAI GTTAAC 1 cut(s) 1921
Kzo9I GATC 9 cut(s) 226, 528, 997, 1251, 1285, 1413, 1748, 2008, 2346
LmnI GCTCC 8 cut(s) 494, 853, 893, 1573, 1731, 1847, 1893, 2194
Lsp1109I GCAGC 8 cut(s) 904, 1133, 1317, 1576, 1625, 1687, 1700, 1726
LweI GCATC 5 cut(s) 198, 815, 1168, 1234, 1256
MaeII ACGT 8 cut(s) 105, 243, 795, 1052, 1899, 1917, 2064, 2127
MaeIII GTNAC 7 cut(s) 95, 818, 1180, 1421, 1501, 2095, 2137
MalI GATC 9 cut(s) 228, 530, 999, 1253, 1287, 1415, 1750, 2010, 2348
MboI GATC 9 cut(s) 226, 528, 997, 1251, 1285, 1413, 1748, 2008, 2346
MboII GAAGA 7 cut(s) 309, 411, 593, 1206, 1321, 2029, 2188
MfeI CAATTG 2 cut(s) 150, 1131
MflI RGATCY 3 cut(s) 997, 1748, 2008
MhlI GDGCHC 6 cut(s) 80, 699, 1682, 1736, 1890, 1936
MlsI TGGCCA 2 cut(s) 559, 866
MluNI TGGCCA 2 cut(s) 559, 866
MlyI GAGTC 3 cut(s) 376, 2045, 2175
MmeI TCCRAC 3 cut(s) 885, 2143, 2163
Mox20I TGGCCA 2 cut(s) 559, 866
Mph1103I ATGCAT 1 cut(s) 1249
MroI TCCGGA 1 cut(s) 2085
MroXI GAANNNNTTC 1 cut(s) 356
MscI TGGCCA 2 cut(s) 559, 866
MseI TTAA 6 cut(s) 395, 576, 687, 1650, 1920, 2013
MslI CAYNNNNRTG 3 cut(s) 191, 607, 728
Msp20I TGGCCA 2 cut(s) 559, 866
MspA1I CMGCKG 3 cut(s) 840, 1124, 1691
MunI CAATTG 2 cut(s) 150, 1131
Mva1269I GAATGC 1 cut(s) 2033
MvaI CCWGG 4 cut(s) 828, 1262, 1572, 1586
MwoI GCNNNNNNNGC 7 cut(s) 408, 854, 863, 1316, 1490, 1697, 1847
NciI CCSGG 6 cut(s) 292, 1139, 1203, 1852, 1884, 1885
NdeII GATC 9 cut(s) 226, 528, 997, 1251, 1285, 1413, 1748, 2008, 2346
NmuCI GTSAC 3 cut(s) 1180, 2095, 2137
NsbI TGCGCA 1 cut(s) 1719
NsiI ATGCAT 1 cut(s) 1249
NspI RCATGY 3 cut(s) 863, 1859, 2303
NspV TTCGAA 3 cut(s) 509, 1146, 2060
PagI TCATGA 2 cut(s) 348, 2077
PceI AGGCCT 1 cut(s) 392
PciI ACATGT 1 cut(s) 2299
PcsI WCGNNNNNNNCGW 1 cut(s) 2133
PctI GAATGC 1 cut(s) 2033
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 2 cut(s) 197, 489
PfoI TCCNGGA 1 cut(s) 1201
PinAI ACCGGT 1 cut(s) 1787
Ple19I CGATCG 1 cut(s) 1254
PleI GAGTC 3 cut(s) 375, 2045, 2174
PpsI GAGTC 3 cut(s) 375, 2045, 2174
Ppu21I YACGTR 2 cut(s) 244, 796
PpuMI RGGWCCY 2 cut(s) 294, 1070
PscI ACATGT 1 cut(s) 2299
PsiI TTATAA 1 cut(s) 1296
Psp5II RGGWCCY 2 cut(s) 294, 1070
Psp6I CCWGG 4 cut(s) 826, 1260, 1570, 1584
PspEI GGTNACC 1 cut(s) 2095
PspGI CCWGG 4 cut(s) 826, 1260, 1570, 1584
PspPI GGNCC 6 cut(s) 294, 1070, 1523, 1545, 1873, 2234
PspPPI RGGWCCY 2 cut(s) 294, 1070
PstI CTGCAG 1 cut(s) 1060
PsuI RGATCY 3 cut(s) 997, 1748, 2008
PvuI CGATCG 1 cut(s) 1254
PvuII CAGCTG 2 cut(s) 1124, 1691
RsaI GTAC 6 cut(s) 242, 763, 872, 1129, 1302, 2067
RsaNI GTAC 6 cut(s) 241, 762, 871, 1128, 1301, 2066
RseI CAYNNNNRTG 3 cut(s) 191, 607, 728
SalI GTCGAC 1 cut(s) 2119
SaqAI TTAA 6 cut(s) 395, 576, 687, 1650, 1920, 2013
Sau3AI GATC 9 cut(s) 226, 528, 997, 1251, 1285, 1413, 1748, 2008, 2346
Sau96I GGNCC 6 cut(s) 294, 1070, 1523, 1545, 1873, 2234
SchI GAGTC 3 cut(s) 376, 2045, 2175
SduI GDGCHC 6 cut(s) 80, 699, 1682, 1736, 1890, 1936
SfaNI GCATC 5 cut(s) 198, 815, 1168, 1234, 1256
SfcI CTRYAG 1 cut(s) 1056
SfuI TTCGAA 3 cut(s) 509, 1146, 2060
SinI GGWCC 4 cut(s) 294, 1070, 1545, 2234
SmaI CCCGGG 1 cut(s) 1885
SmiMI CAYNNNNRTG 3 cut(s) 191, 607, 728
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
SseBI AGGCCT 1 cut(s) 392
SsiI CCGC 5 cut(s) 783, 840, 1380, 1611, 1752
SspI AATATT 1 cut(s) 1906
StuI AGGCCT 1 cut(s) 392
StyI CCWWGG 3 cut(s) 851, 1026, 1103
TaaI ACNGT 6 cut(s) 944, 1117, 1659, 1991, 2095, 2118
TaiI ACGT 8 cut(s) 108, 246, 798, 1055, 1902, 1920, 2067, 2130
TaqI TCGA 8 cut(s) 509, 1146, 1250, 1982, 2054, 2060, 2120, 2190
TatI WGTACW 1 cut(s) 870
TauI GCSGC 2 cut(s) 785, 843
TfiI GAWTC 2 cut(s) 197, 489
Tru1I TTAA 6 cut(s) 395, 576, 687, 1650, 1920, 2013
Tru9I TTAA 6 cut(s) 395, 576, 687, 1650, 1920, 2013
TscAI CASTG 4 cut(s) 367, 379, 429, 2262
TseFI GTSAC 3 cut(s) 1180, 2095, 2137
TseI GCWGC 8 cut(s) 917, 1121, 1305, 1589, 1613, 1688, 1700, 1739
Tsp45I GTSAC 3 cut(s) 1180, 2095, 2137
TspMI CCCGGG 1 cut(s) 1883
TspRI CASTG 4 cut(s) 367, 379, 429, 2262
VneI GTGCAC 1 cut(s) 1932
VpaK11BI GGWCC 4 cut(s) 294, 1070, 1545, 2234
XapI RAATTY 5 cut(s) 733, 1142, 1207, 2056, 2081
XbaI TCTAGA 1 cut(s) 1517
XceI RCATGY 3 cut(s) 863, 1859, 2303
XmaI CCCGGG 1 cut(s) 1883
XmaJI CCTAGG 1 cut(s) 851
XmiI GTMKAC 3 cut(s) 776, 1190, 2120
XmnI GAANNNNTTC 1 cut(s) 356
Zsp2I ATGCAT 1 cut(s) 1249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.