pycom04g21070

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001268
Physical Location & Seq
Forward (+)
22419747 .. 22422029
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g21070.1

Sequence Viewer

Length: 2283 bp
ATGGGGCATAACAATGGGGTTCCTCTTCCCTTCATTTTCTCTCTTGTTGCATTGTTTCTGTTGGCTCTTCAAGTCAACGTGTCTTTCGCAGATAGGTCGACATATATTGTCCACATGGACAAGTCCCTCATGCCCAAGTCCTACACTAGCCATGACCATTGGTACTCCTCCATCGTCGATTCATTCAAATCCGAGAACCCTACCTCGTTCGATGGCAACAAAATCTTGCCTTCCATTCTGTACACCTATGACAATGCCTTTCATGGGTTCAGTGCAGTGCTATCTGCTGATGAATTAGTGACTCTAAAAAAGTCCCCGGGTTTCGTCTCAGCTTACGCTGACAAGTCCATTACGCTCGACACCACCCACACCACTCAGTTCCTCAACCTCAATCCTTTCGCCGGACTGTGGCCTGCTTCGGATTACGGAGACGACATCATCATTGGTGTCATTGATACCGGTCTCTGGCCGGAGAGTGAAAGCTTCAGAGATGAAGGTATGACCAAAAGTCTTCCAGCTAGGTGGAAAGGAAAATGTGAGGTTGGACAAGACTTCAATGCCTCTCTTTGTAACTATAAATTGATTGGAGCTCGATACTTTAACAAGGGTGTCATGGCTGCAAACCCCGGTGTCACACTCAGCATGAACTCGGCTAGAGACAGTGCAGGGCATGGGACGCATACATCTTCCACAGCTGCGGGGAACTACGTGGACGGGGCATCTTATTTTGGTTACGCTAAAGGAACAGCCAGAGGCGTAGCACCGCGTGCCAGAGTTGCCATGTACAAGGTTATCTGGGATGAGGGGCGTTATGCCTCTGATGTTCTAGCCGGAATGGACCAAGCTATTGCTGATGGTGTTGATGTTATTTCAATTTCCTCTGGCTTTTACGACACACCATTGTACGAGGATCCCGTAGCGATTGCTTCATTTGCAGCCATGGAGAAGGGTGTGGTGGTTTCAGCTTCAGCCGGAAATGAAGGCCCCGAGCTTGGGAAATTGCACAATGGCATCCCTTGGGTCTTGACCGTTGCAGCCGGCACAATAGACCGCTCATTTGGAGGAGTATTAACCTTTGGTAATGGTTTAACCATTACTGGATTTACCTTGTTCCCAGCAAACGCCTTAGTAAAAAACTCGCCACTGGTTTACAACAAGACCTTCTCAGCATGCAACTCAACAGCAGCGCTAGCAACTGCACCAGATGCGATCATCATATGTGATGGCACGGAGCCTATTTATTATCAAATATCTCACATCATTCGGTCGGGGCTTCTTGGAGCTATCTTTATTACCAATAATCCTGAGATACGCGAATTAGGTTACGTTGCAACTCCCAGCGTTGTGGTGAATACGAAGGATGGGCGGACTGTGATCGAGTACGCACTAAAAAGTGAAAACCCTACTGTCAGCATAAATTTCCAACAAACGTTGCTCAATACAAAGCCTGCACCCGCTGCTGCTTCTTACACTTCGAGAGGTCCTTCAAAAAGTTATCCGGGCATTTTGAAGCCGGACATAATGGCCCCGGGGTCATTAGTTTTAGCTGCTTGGGTTCCCAAGGTTGCAGCGGGTAAGATCGGGTTCAATGTGAACTTACCTAGCGACTACAATTTGATATCCGGGACATCCATGGCATGCCCTCATGCTTCAGGTGTAGCTGCTCTACTGAAAGGTGCGCACCCGGAATGGAGTGCAGCAGCAATTAGATCTGCTTTGATGACCACAGCTAACCCATTGGACAATACTGGCAATCCAATTCTTGACGACGGTGACAATTTCAACTTTGCTTCACCATTAGCTATGGGAGCAGGCCACATCGATCCTAATAGAACACTCGATCCGGGCCTAATATATGATGCTACTCCACAAGAATATGTCAATCTCTTATGCTCCACGAACTTTACTCGTAACCAAATCTTATCCATCACTAGATCGCACAACTATGATTATTCCAAACCATCTTCTGATCTCAACTATCCATCTTTCATTGCGTTGTCCAACAATCATCACAAAACAAAGACAAGGGTTCAAACATTTCAGAGGACCGTAACAAATGTGGGAGATGGTGCGGCTAGGTACAGGGCTTCGGTGACTTCTCCAAAGGGTTCGCAGGTAACAGTCTCCCCAGAGATACTGATCTTTGCATACAAGTATGAGAAGCAAAGTTTTACGGTCACTTTAAATTACAAAGCGAAGAAGAAGGGGAAGGCTTCTTCTGGTGCACTTGTTTGGATCGAACAAAATGGAAAATACACTGTCAGGAGCCCGATTGTAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

761

Amino Acids

81.5

Weight (kDa)

7.25

Isoelectric Point (pI)

31.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 119 1.6e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 143 - 581 1.8e-43 Subtilase family
fn3_6 PF17766 657 - 759 3.9e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1680
Acc36I ACCTGC 1 cut(s) 2104
AccBSI CCGCTC 1 cut(s) 1053
AccI GTMKAC 1 cut(s) 98
AccII CGCG 2 cut(s) 766, 1314
AciI CCGC 7 cut(s) 698, 764, 1051, 1366, 1455, 1571, 2072
AclI AACGTT 1 cut(s) 1430
AclWI GGATC 5 cut(s) 905, 918, 1817, 1835, 2243
AcoI YGGCCR 1 cut(s) 467
AcsI RAATTY 1 cut(s) 1417
AcuI CTGAAG 3 cut(s) 469, 951, 1635
AdeI CACNNNGTG 1 cut(s) 767
AfaI GTAC 6 cut(s) 164, 242, 785, 905, 1382, 2081
AfeI AGCGCT 1 cut(s) 1188
AfiI CCNNNNNNNGG 6 cut(s) 264, 401, 408, 465, 992, 993
AflIII ACRYGT 1 cut(s) 78
AgeI ACCGGT 1 cut(s) 458
AgsI TTSAA 9 cut(s) 71, 187, 556, 873, 1488, 1510, 1588, 1783, 2033
AhdI GACNNNNNGTC 1 cut(s) 507
AloI GAACNNNNNNTCC 2 cut(s) 1825, 1857
Alw21I GWGCWC 2 cut(s) 592, 2227
Alw26I GTCTC 5 cut(s) 331, 423, 467, 651, 2128
Alw44I GTGCAC 1 cut(s) 2223
AlwI GGATC 5 cut(s) 905, 918, 1817, 1835, 2243
Ama87I CYCGRG 3 cut(s) 316, 986, 1528
Aor51HI AGCGCT 1 cut(s) 1188
AoxI GGCC 6 cut(s) 410, 467, 982, 1524, 1813, 1846
ApaLI GTGCAC 1 cut(s) 2223
ApoI RAATTY 1 cut(s) 1417
ArsI GACNNNNNNTTYG 2 cut(s) 1040, 1072
AsiGI ACCGGT 1 cut(s) 458
AspLEI GCGC 2 cut(s) 1189, 1681
AspS9I GGNCC 6 cut(s) 838, 983, 1481, 1525, 1846, 2046
AsuC2I CCSGG 9 cut(s) 317, 318, 627, 1500, 1529, 1530, 1624, 1685, 1845
AsuHPI GGTGA 4 cut(s) 1360, 1784, 1785, 2104
AsuNHI GCTAGC 1 cut(s) 1189
AvaI CYCGRG 3 cut(s) 316, 986, 1528
AvaII GGWCC 3 cut(s) 838, 1481, 2046
BaeGI GKGCMC 1 cut(s) 2227
BamHI GGATCC 1 cut(s) 910
BanII GRGCYC 2 cut(s) 592, 2270
BbsI GAAGAC 1 cut(s) 503
Bbv12I GWGCWC 2 cut(s) 592, 2227
BccI CCATC 9 cut(s) 179, 206, 848, 1217, 1355, 1934, 1969, 1990, 2060
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BcnI CCSGG 9 cut(s) 317, 318, 627, 1500, 1529, 1530, 1624, 1685, 1845
BcoDI GTCTC 5 cut(s) 331, 423, 467, 651, 2128
BfaI CTAG 8 cut(s) 147, 519, 654, 827, 1190, 1602, 1932, 2076
BfoI RGCGCY 1 cut(s) 1190
BfuAI ACCTGC 1 cut(s) 2104
BglII AGATCT 1 cut(s) 1709
Bme1390I CCNGG 9 cut(s) 317, 318, 627, 1500, 1529, 1530, 1624, 1685, 1845
Bme18I GGWCC 3 cut(s) 838, 1481, 2046
BmeRI GACNNNNNGTC 1 cut(s) 507
BmeT110I CYCGRG 3 cut(s) 316, 986, 1528
BmgT120I GGNCC 6 cut(s) 838, 983, 1481, 1525, 1846, 2046
BmiI GGNNCC 7 cut(s) 21, 912, 985, 1233, 1527, 1557, 2267
BmrFI CCNGG 9 cut(s) 317, 318, 627, 1500, 1529, 1530, 1624, 1685, 1845
BmsI GCATC 4 cut(s) 728, 1020, 1195, 1849
BmtI GCTAGC 1 cut(s) 1193
BpiI GAAGAC 1 cut(s) 503
BpuMI CCSGG 9 cut(s) 317, 318, 627, 1500, 1529, 1530, 1624, 1685, 1845
Bsa29I ATCGAT 1 cut(s) 1821
BsaAI YACGTR 1 cut(s) 709
BsaBI GATNNNNATC 1 cut(s) 2138
BsaI GGTCTC 1 cut(s) 467
BsaWI WCCGGW 1 cut(s) 458
Bsc4I CCNNNNNNNGG 6 cut(s) 264, 401, 408, 465, 992, 993
Bse118I RCCGGY 2 cut(s) 458, 1037
Bse1I ACTGG 3 cut(s) 1102, 1149, 1753
Bse3DI GCAATG 1 cut(s) 1989
Bse8I GATNNNNATC 1 cut(s) 2138
BseCI ATCGAT 1 cut(s) 1821
BseGI GGATG 4 cut(s) 805, 1011, 1366, 1628
BseJI GATNNNNATC 1 cut(s) 2138
BseLI CCNNNNNNNGG 6 cut(s) 264, 401, 408, 465, 992, 993
BseMI GCAATG 1 cut(s) 1989
BseMII CTCAG 5 cut(s) 342, 389, 652, 1179, 1296
BseNI ACTGG 3 cut(s) 1102, 1149, 1753
BseRI GAGGAG 2 cut(s) 157, 1077
BseSI GKGCMC 1 cut(s) 2227
BseYI CCCAGC 2 cut(s) 1114, 1337
BsgI GTGCAG 5 cut(s) 294, 684, 1182, 1434, 1716
Bsh1236I CGCG 2 cut(s) 766, 1314
Bsh1285I CGRYCG 1 cut(s) 1268
BshFI GGCC 6 cut(s) 412, 469, 984, 1526, 1815, 1848
BshTI ACCGGT 1 cut(s) 458
BshVI ATCGAT 1 cut(s) 1821
BsiEI CGRYCG 1 cut(s) 1268
BsiHKAI GWGCWC 2 cut(s) 592, 2227
BsiHKCI CYCGRG 3 cut(s) 316, 986, 1528
BslFI GGGAC 4 cut(s) 109, 298, 688, 1639
BslI CCNNNNNNNGG 6 cut(s) 264, 401, 408, 465, 992, 993
BsmAI GTCTC 5 cut(s) 331, 423, 467, 651, 2128
BsmBI CGTCTC 2 cut(s) 331, 423
BsmFI GGGAC 4 cut(s) 109, 298, 688, 1639
BsnI GGCC 6 cut(s) 412, 469, 984, 1526, 1815, 1848
Bso31I GGTCTC 1 cut(s) 467
BsoBI CYCGRG 3 cut(s) 316, 986, 1528
Bsp1286I GDGCHC 3 cut(s) 592, 2227, 2270
Bsp1407I TGTACA 2 cut(s) 240, 783
Bsp19I CCATGG 2 cut(s) 939, 1632
BspACI CCGC 7 cut(s) 698, 764, 1051, 1366, 1455, 1571, 2072
BspANI GGCC 6 cut(s) 412, 469, 984, 1526, 1815, 1848
BspCNI CTCAG 5 cut(s) 341, 388, 651, 1178, 1297
BspDI ATCGAT 1 cut(s) 1821
BspFNI CGCG 2 cut(s) 766, 1314
BspLI GGNNCC 7 cut(s) 21, 912, 985, 1233, 1527, 1557, 2267
BspMI ACCTGC 1 cut(s) 2104
BspOI GCTAGC 1 cut(s) 1193
BspPI GGATC 5 cut(s) 905, 918, 1817, 1835, 2243
BspQI GCTCTTC 1 cut(s) 72
BspTNI GGTCTC 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 1053
BsrDI GCAATG 1 cut(s) 1989
BsrFI RCCGGY 2 cut(s) 458, 1037
BsrGI TGTACA 2 cut(s) 240, 783
BsrI ACTGG 3 cut(s) 1102, 1149, 1753
BssAI RCCGGY 2 cut(s) 458, 1037
BssT1I CCWWGG 4 cut(s) 939, 1016, 1560, 1632
Bst6I CTCTTC 2 cut(s) 30, 72
BstAPI GCANNNNNTGC 3 cut(s) 767, 1205, 1457
BstAUI TGTACA 2 cut(s) 240, 783
BstBAI YACGTR 1 cut(s) 709
BstC8I GCNNGC 8 cut(s) 414, 768, 1039, 1171, 1191, 1449, 1639, 1813
BstDEI CTNAG 6 cut(s) 328, 375, 638, 1126, 1165, 1305
BstDSI CCRYGG 2 cut(s) 939, 1632
BstF5I GGATG 4 cut(s) 805, 1011, 1366, 1628
BstFNI CGCG 2 cut(s) 766, 1314
BstH2I RGCGCY 1 cut(s) 1190
BstHHI GCGC 2 cut(s) 1189, 1681
BstMAI GTCTC 5 cut(s) 331, 423, 467, 651, 2128
BstMCI CGRYCG 1 cut(s) 1268
BstMWI GCNNNNNNNGC 8 cut(s) 676, 767, 776, 932, 1190, 1205, 1457, 1808
BstNSI RCATGY 2 cut(s) 1173, 1641
BstSCI CCNGG 9 cut(s) 315, 316, 625, 1498, 1527, 1528, 1622, 1683, 1843
BstSLI GKGCMC 1 cut(s) 2227
BstUI CGCG 2 cut(s) 766, 1314
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 2 cut(s) 910, 1709
BstXI CCANNNNNNTGG 2 cut(s) 522, 1345
BstYI RGATCY 2 cut(s) 910, 1709
Bsu15I ATCGAT 1 cut(s) 1821
BsuRI GGCC 6 cut(s) 412, 469, 984, 1526, 1815, 1848
BsuTUI ATCGAT 1 cut(s) 1821
BtgI CCRYGG 2 cut(s) 939, 1632
BtsCI GGATG 4 cut(s) 805, 1011, 1366, 1628
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 5 cut(s) 277, 282, 667, 1142, 2256
BveI ACCTGC 1 cut(s) 2104
Cac8I GCNNGC 8 cut(s) 414, 768, 1039, 1171, 1191, 1449, 1639, 1813
CfoI GCGC 2 cut(s) 1189, 1681
Cfr10I RCCGGY 2 cut(s) 458, 1037
Cfr13I GGNCC 6 cut(s) 838, 983, 1481, 1525, 1846, 2046
Cfr9I CCCGGG 2 cut(s) 316, 1528
ClaI ATCGAT 1 cut(s) 1821
CseI GACGC 1 cut(s) 685
Csp6I GTAC 6 cut(s) 163, 241, 784, 904, 1381, 2080
CspAI ACCGGT 1 cut(s) 458
CviQI GTAC 6 cut(s) 163, 241, 784, 904, 1381, 2080
DdeI CTNAG 6 cut(s) 328, 375, 638, 1126, 1165, 1305
DraI TTTAAA 1 cut(s) 2184
DraIII CACNNNGTG 1 cut(s) 767
DriI GACNNNNNGTC 1 cut(s) 507
EaeI YGGCCR 1 cut(s) 467
Eam1104I CTCTTC 2 cut(s) 30, 72
Eam1105I GACNNNNNGTC 1 cut(s) 507
EarI CTCTTC 2 cut(s) 30, 72
EciI GGCGGA 1 cut(s) 1381
Ecl136II GAGCTC 1 cut(s) 590
Eco130I CCWWGG 4 cut(s) 939, 1016, 1560, 1632
Eco24I GRGCYC 2 cut(s) 592, 2270
Eco31I GGTCTC 1 cut(s) 467
Eco32I GATATC 1 cut(s) 1620
Eco47I GGWCC 3 cut(s) 838, 1481, 2046
Eco47III AGCGCT 1 cut(s) 1188
Eco53kI GAGCTC 1 cut(s) 590
Eco57I CTGAAG 3 cut(s) 469, 951, 1635
Eco88I CYCGRG 3 cut(s) 316, 986, 1528
EcoICRI GAGCTC 1 cut(s) 590
EcoO109I RGGNCCY 2 cut(s) 983, 1481
EcoRV GATATC 1 cut(s) 1620
EcoT14I CCWWGG 4 cut(s) 939, 1016, 1560, 1632
EcoT38I GRGCYC 2 cut(s) 592, 2270
ErhI CCWWGG 4 cut(s) 939, 1016, 1560, 1632
Esp3I CGTCTC 2 cut(s) 331, 423
FaqI GGGAC 4 cut(s) 109, 298, 688, 1639
FauI CCCGC 3 cut(s) 691, 1462, 1564
FauNDI CATATG 1 cut(s) 1217
FblI GTMKAC 1 cut(s) 98
FokI GGATG 4 cut(s) 812, 998, 1373, 1615
FriOI GRGCYC 2 cut(s) 592, 2270
FspAI RTGCGCAY 1 cut(s) 1680
FspBI CTAG 8 cut(s) 147, 519, 654, 827, 1190, 1602, 1932, 2076
FspI TGCGCA 1 cut(s) 1680
GlaI GCGC 2 cut(s) 1188, 1680
GsaI CCCAGC 2 cut(s) 1118, 1341
HaeII RGCGCY 1 cut(s) 1190
HaeIII GGCC 6 cut(s) 412, 469, 984, 1526, 1815, 1848
HgaI GACGC 1 cut(s) 685
HhaI GCGC 2 cut(s) 1189, 1681
Hin6I GCGC 2 cut(s) 1187, 1679
HinP1I GCGC 2 cut(s) 1187, 1679
HincII GTYRAC 2 cut(s) 76, 99
HindII GTYRAC 2 cut(s) 76, 99
HindIII AAGCTT 1 cut(s) 481
HinfI GANTC 2 cut(s) 179, 301
HphI GGTGA 4 cut(s) 1360, 1784, 1785, 2104
Hpy166II GTNNAC 8 cut(s) 76, 99, 112, 243, 712, 1150, 1594, 2225
Hpy188I TCNGA 6 cut(s) 193, 421, 488, 820, 1969, 2043
Hpy188III TCNNGA 5 cut(s) 1024, 1304, 1476, 1763, 2263
Hpy8I GTNNAC 8 cut(s) 76, 99, 112, 243, 712, 1150, 1594, 2225
Hpy99I CGWCG 2 cut(s) 179, 1772
HpyCH4IV ACGT 4 cut(s) 78, 708, 1326, 1430
HpyF10VI GCNNNNNNNGC 8 cut(s) 676, 767, 776, 932, 1190, 1205, 1457, 1808
HpyF3I CTNAG 6 cut(s) 328, 375, 638, 1126, 1165, 1305
HpySE526I ACGT 4 cut(s) 78, 708, 1326, 1430
HspAI GCGC 2 cut(s) 1187, 1679
KroI GCCGGC 1 cut(s) 1037
KroNI GCCGGC 1 cut(s) 1039
LguI GCTCTTC 1 cut(s) 72
LmnI GCTCC 6 cut(s) 587, 1231, 1280, 1808, 1898, 2265
LweI GCATC 4 cut(s) 728, 1020, 1195, 1849
MaeI CTAG 8 cut(s) 147, 519, 654, 827, 1190, 1602, 1932, 2076
MaeII ACGT 4 cut(s) 78, 708, 1326, 1430
MbiI CCGCTC 1 cut(s) 1053
MboII GAAGA 8 cut(s) 17, 59, 503, 678, 1956, 2208, 2209, 2212
MflI RGATCY 2 cut(s) 910, 1709
MhlI GDGCHC 3 cut(s) 592, 2227, 2270
MlyI GAGTC 1 cut(s) 295
MmeI TCCRAC 3 cut(s) 523, 1447, 2025
MroNI GCCGGC 1 cut(s) 1037
MseI TTAA 4 cut(s) 600, 1070, 1088, 2183
MslI CAYNNNNRTG 2 cut(s) 12, 1944
MspA1I CMGCKG 3 cut(s) 695, 1457, 1571
MspR9I CCNGG 9 cut(s) 317, 318, 627, 1500, 1529, 1530, 1624, 1685, 1845
MvnI CGCG 2 cut(s) 766, 1314
MwoI GCNNNNNNNGC 8 cut(s) 676, 767, 776, 932, 1190, 1205, 1457, 1808
NaeI GCCGGC 1 cut(s) 1039
NciI CCSGG 9 cut(s) 317, 318, 627, 1500, 1529, 1530, 1624, 1685, 1845
NcoI CCATGG 2 cut(s) 939, 1632
NdeI CATATG 1 cut(s) 1217
NgoMIV GCCGGC 1 cut(s) 1037
NheI GCTAGC 1 cut(s) 1189
NlaIV GGNNCC 7 cut(s) 21, 912, 985, 1233, 1527, 1557, 2267
NmeAIII GCCGAG 1 cut(s) 629
NmuCI GTSAC 5 cut(s) 298, 631, 1772, 2092, 2176
NsbI TGCGCA 1 cut(s) 1680
NspI RCATGY 2 cut(s) 1173, 1641
PaeI GCATGC 2 cut(s) 1173, 1641
PciSI GCTCTTC 1 cut(s) 72
PcsI WCGNNNNNNNCGW 1 cut(s) 912
PdiI GCCGGC 1 cut(s) 1039
PfeI GAWTC 1 cut(s) 179
PfoI TCCNGGA 1 cut(s) 1622
PinAI ACCGGT 1 cut(s) 458
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Ppu21I YACGTR 1 cut(s) 709
PpuMI RGGWCCY 1 cut(s) 1481
Psp124BI GAGCTC 1 cut(s) 592
Psp1406I AACGTT 1 cut(s) 1430
Psp5II RGGWCCY 1 cut(s) 1481
PspFI CCCAGC 2 cut(s) 1114, 1337
PspN4I GGNNCC 7 cut(s) 21, 912, 985, 1233, 1527, 1557, 2267
PspPI GGNCC 6 cut(s) 838, 983, 1481, 1525, 1846, 2046
PspPPI RGGWCCY 1 cut(s) 1481
PsuI RGATCY 2 cut(s) 910, 1709
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 6 cut(s) 164, 242, 785, 905, 1382, 2081
RsaNI GTAC 6 cut(s) 163, 241, 784, 904, 1381, 2080
RseI CAYNNNNRTG 2 cut(s) 12, 1944
SacI GAGCTC 1 cut(s) 592
SalI GTCGAC 1 cut(s) 97
SapI GCTCTTC 1 cut(s) 72
SaqAI TTAA 4 cut(s) 600, 1070, 1088, 2183
Sau96I GGNCC 6 cut(s) 838, 983, 1481, 1525, 1846, 2046
SchI GAGTC 1 cut(s) 295
ScrFI CCNGG 9 cut(s) 317, 318, 627, 1500, 1529, 1530, 1624, 1685, 1845
SduI GDGCHC 3 cut(s) 592, 2227, 2270
SfaNI GCATC 4 cut(s) 728, 1020, 1195, 1849
SinI GGWCC 3 cut(s) 838, 1481, 2046
SmaI CCCGGG 2 cut(s) 318, 1530
SmiMI CAYNNNNRTG 2 cut(s) 12, 1944
SphI GCATGC 2 cut(s) 1173, 1641
SsiI CCGC 7 cut(s) 698, 764, 1051, 1366, 1455, 1571, 2072
SspMI CTAG 8 cut(s) 147, 519, 654, 827, 1190, 1602, 1932, 2076
SstI GAGCTC 1 cut(s) 592
StyD4I CCNGG 9 cut(s) 315, 316, 625, 1498, 1527, 1528, 1622, 1683, 1843
StyI CCWWGG 4 cut(s) 939, 1016, 1560, 1632
TaiI ACGT 4 cut(s) 81, 711, 1329, 1433
TaqII GACCGA 1 cut(s) 1254
TatI WGTACW 2 cut(s) 240, 783
TauI GCSGC 1 cut(s) 2075
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 4 cut(s) 600, 1070, 1088, 2183
Tru9I TTAA 4 cut(s) 600, 1070, 1088, 2183
TscAI CASTG 5 cut(s) 277, 282, 667, 1149, 2263
TseFI GTSAC 5 cut(s) 298, 631, 1772, 2092, 2176
Tsp45I GTSAC 5 cut(s) 298, 631, 1772, 2092, 2176
TspDTI ATGAA 9 cut(s) 22, 171, 251, 306, 507, 659, 918, 993, 1978
TspGWI ACGGA 2 cut(s) 441, 1244
TspMI CCCGGG 2 cut(s) 316, 1528
TspRI CASTG 5 cut(s) 277, 282, 667, 1149, 2263
VneI GTGCAC 1 cut(s) 2223
VpaK11BI GGWCC 3 cut(s) 838, 1481, 2046
XapI RAATTY 1 cut(s) 1417
XceI RCATGY 2 cut(s) 1173, 1641
XmaI CCCGGG 2 cut(s) 316, 1528
XmiI GTMKAC 1 cut(s) 98
XspI CTAG 8 cut(s) 147, 519, 654, 827, 1190, 1602, 1932, 2076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.