subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1530 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1075 |
| AccI | GTMKAC | 1 cut(s) | 616 |
| AccII | CGCG | 2 cut(s) | 139, 1290 |
| AciI | CCGC | 4 cut(s) | 87, 534, 746, 1682 |
| AclI | AACGTT | 1 cut(s) | 732 |
| AclWI | GGATC | 2 cut(s) | 781, 910 |
| AcoI | YGGCCR | 1 cut(s) | 1426 |
| AcsI | RAATTY | 3 cut(s) | 190, 307, 1055 |
| AcuI | CTGAAG | 1 cut(s) | 75 |
| AfaI | GTAC | 3 cut(s) | 410, 792, 1367 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 512, 1075 |
| AflIII | ACRYGT | 1 cut(s) | 158 |
| AgeI | ACCGGT | 1 cut(s) | 20 |
| AgsI | TTSAA | 8 cut(s) | 307, 331, 738, 1055, 1196, 1358, 1464, 1502 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 937, 969 |
| Alw26I | GTCTC | 2 cut(s) | 463, 1148 |
| AlwI | GGATC | 2 cut(s) | 781, 910 |
| Ama87I | CYCGRG | 1 cut(s) | 269 |
| AoxI | GGCC | 2 cut(s) | 1426, 1558 |
| ApeKI | GCWGC | 4 cut(s) | 265, 433, 714, 996 |
| ApoI | RAATTY | 3 cut(s) | 190, 307, 1055 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 39, 71 |
| AsiGI | ACCGGT | 1 cut(s) | 20 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 191, 733 |
| AspLEI | GCGC | 4 cut(s) | 141, 759, 768, 1531 |
| AspS9I | GGNCC | 2 cut(s) | 444, 1645 |
| AsuC2I | CCSGG | 1 cut(s) | 442 |
| AsuHPI | GGTGA | 5 cut(s) | 33, 356, 619, 833, 1412 |
| AvaI | CYCGRG | 1 cut(s) | 269 |
| AvaII | GGWCC | 2 cut(s) | 444, 1645 |
| BalI | TGGCCA | 1 cut(s) | 1428 |
| BbvI | GCAGC | 4 cut(s) | 252, 420, 701, 983 |
| BccI | CCATC | 6 cut(s) | 1094, 1232, 1433, 1437, 1615, 1637 |
| BciVI | GTATCC | 1 cut(s) | 220 |
| BcnI | CCSGG | 1 cut(s) | 442 |
| BcoDI | GTCTC | 2 cut(s) | 463, 1148 |
| BfaI | CTAG | 3 cut(s) | 420, 1010, 1205 |
| BfmI | CTRYAG | 2 cut(s) | 1245, 1591 |
| BfuI | GTATCC | 1 cut(s) | 220 |
| BisI | GCNGC | 4 cut(s) | 266, 434, 715, 997 |
| BlsI | GCNGC | 4 cut(s) | 267, 435, 716, 998 |
| Bme1390I | CCNGG | 1 cut(s) | 442 |
| Bme18I | GGWCC | 2 cut(s) | 444, 1645 |
| BmeT110I | CYCGRG | 1 cut(s) | 269 |
| BmgT120I | GGNCC | 2 cut(s) | 444, 1645 |
| BmiI | GGNNCC | 2 cut(s) | 787, 1647 |
| BmrFI | CCNGG | 1 cut(s) | 442 |
| BmsI | GCATC | 8 cut(s) | 256, 390, 445, 626, 1277, 1459, 1540, 1629 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 443, 475 |
| BpuEI | CTTGAG | 2 cut(s) | 242, 492 |
| BpuMI | CCSGG | 1 cut(s) | 442 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1443 |
| BsaI | GGTCTC | 1 cut(s) | 463 |
| BsaJI | CCNNGG | 5 cut(s) | 440, 673, 921, 1215, 1561 |
| BsaWI | WCCGGW | 1 cut(s) | 20 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 512, 1075 |
| Bse118I | RCCGGY | 1 cut(s) | 20 |
| Bse1I | ACTGG | 3 cut(s) | 526, 696, 868 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1443 |
| BseDI | CCNNGG | 5 cut(s) | 440, 673, 921, 1215, 1561 |
| BseGI | GGATG | 5 cut(s) | 370, 405, 436, 641, 1105 |
| BseJI | GATNNNNATC | 1 cut(s) | 1443 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 512, 1075 |
| BseMII | CTCAG | 2 cut(s) | 46, 441 |
| BseNI | ACTGG | 3 cut(s) | 526, 696, 868 |
| BseRI | GAGGAG | 1 cut(s) | 467 |
| BseXI | GCAGC | 4 cut(s) | 252, 420, 701, 983 |
| BsgI | GTGCAG | 2 cut(s) | 299, 1569 |
| Bsh1236I | CGCG | 2 cut(s) | 139, 1290 |
| Bsh1285I | CGRYCG | 1 cut(s) | 25 |
| BshFI | GGCC | 2 cut(s) | 1428, 1560 |
| BshTI | ACCGGT | 1 cut(s) | 20 |
| BsiEI | CGRYCG | 1 cut(s) | 25 |
| BsiHKCI | CYCGRG | 1 cut(s) | 269 |
| BsiSI | CCGG | 3 cut(s) | 21, 66, 442 |
| BslFI | GGGAC | 2 cut(s) | 856, 1283 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 512, 1075 |
| BsmAI | GTCTC | 2 cut(s) | 463, 1148 |
| BsmFI | GGGAC | 2 cut(s) | 856, 1283 |
| BsmI | GAATGC | 2 cut(s) | 292, 1274 |
| BsnI | GGCC | 2 cut(s) | 1428, 1560 |
| Bso31I | GGTCTC | 1 cut(s) | 463 |
| BsoBI | CYCGRG | 1 cut(s) | 269 |
| Bsp143I | GATC | 3 cut(s) | 773, 902, 1369 |
| Bsp19I | CCATGG | 1 cut(s) | 1215 |
| BspACI | CCGC | 4 cut(s) | 87, 534, 746, 1682 |
| BspANI | GGCC | 2 cut(s) | 1428, 1560 |
| BspCNI | CTCAG | 2 cut(s) | 45, 442 |
| BspFNI | CGCG | 2 cut(s) | 139, 1290 |
| BspLI | GGNNCC | 2 cut(s) | 787, 1647 |
| BspPI | GGATC | 2 cut(s) | 781, 910 |
| BspTNI | GGTCTC | 1 cut(s) | 463 |
| BsrFI | RCCGGY | 1 cut(s) | 20 |
| BsrI | ACTGG | 3 cut(s) | 526, 696, 868 |
| BssAI | RCCGGY | 1 cut(s) | 20 |
| BssECI | CCNNGG | 5 cut(s) | 440, 673, 921, 1215, 1561 |
| BssMI | GATC | 3 cut(s) | 773, 902, 1369 |
| BssNAI | GTATAC | 1 cut(s) | 617 |
| BssT1I | CCWWGG | 4 cut(s) | 673, 921, 1215, 1561 |
| Bst1107I | GTATAC | 1 cut(s) | 617 |
| Bst4CI | ACNGT | 4 cut(s) | 479, 790, 1473, 1612 |
| Bst6I | CTCTTC | 1 cut(s) | 363 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 120 |
| BstC8I | GCNNGC | 3 cut(s) | 378, 979, 1598 |
| BstDEI | CTNAG | 4 cut(s) | 11, 32, 450, 697 |
| BstDSI | CCRYGG | 1 cut(s) | 1215 |
| BstF5I | GGATG | 5 cut(s) | 370, 405, 436, 641, 1105 |
| BstFNI | CGCG | 2 cut(s) | 139, 1290 |
| BstHHI | GCGC | 4 cut(s) | 141, 759, 768, 1531 |
| BstKTI | GATC | 3 cut(s) | 776, 905, 1372 |
| BstMAI | GTCTC | 2 cut(s) | 463, 1148 |
| BstMBI | GATC | 3 cut(s) | 773, 902, 1369 |
| BstMCI | CGRYCG | 1 cut(s) | 25 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 120, 433, 763, 765, 974 |
| BstNSI | RCATGY | 3 cut(s) | 162, 838, 981 |
| BstSCI | CCNGG | 1 cut(s) | 440 |
| BstSFI | CTRYAG | 2 cut(s) | 1245, 1591 |
| BstUI | CGCG | 2 cut(s) | 139, 1290 |
| BstV1I | GCAGC | 4 cut(s) | 252, 420, 701, 983 |
| BstX2I | RGATCY | 1 cut(s) | 902 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 493, 725 |
| BstYI | RGATCY | 1 cut(s) | 902 |
| BstZ17I | GTATAC | 1 cut(s) | 617 |
| BsuI | GTATCC | 1 cut(s) | 220 |
| BsuRI | GGCC | 2 cut(s) | 1428, 1560 |
| BtgI | CCRYGG | 1 cut(s) | 1215 |
| BtsCI | GGATG | 5 cut(s) | 370, 405, 436, 641, 1105 |
| BtsI | GCAGTG | 1 cut(s) | 1171 |
| BtsIMutI | CAGTG | 4 cut(s) | 447, 689, 963, 1171 |
| Cac8I | GCNNGC | 3 cut(s) | 378, 979, 1598 |
| CfoI | GCGC | 4 cut(s) | 141, 759, 768, 1531 |
| Cfr10I | RCCGGY | 1 cut(s) | 20 |
| Cfr13I | GGNCC | 2 cut(s) | 444, 1645 |
| Csp6I | GTAC | 3 cut(s) | 409, 791, 1366 |
| CspAI | ACCGGT | 1 cut(s) | 20 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 592, 627, 730, 765 |
| CviAII | CATG | 7 cut(s) | 159, 782, 835, 978, 1070, 1216, 1556 |
| CviQI | GTAC | 3 cut(s) | 409, 791, 1366 |
| DdeI | CTNAG | 4 cut(s) | 11, 32, 450, 697 |
| DpnI | GATC | 3 cut(s) | 775, 904, 1371 |
| DpnII | GATC | 3 cut(s) | 773, 902, 1369 |
| EaeI | YGGCCR | 1 cut(s) | 1426 |
| Eam1104I | CTCTTC | 1 cut(s) | 363 |
| EarI | CTCTTC | 1 cut(s) | 363 |
| EciI | GGCGGA | 1 cut(s) | 549 |
| Eco130I | CCWWGG | 4 cut(s) | 673, 921, 1215, 1561 |
| Eco31I | GGTCTC | 1 cut(s) | 463 |
| Eco32I | GATATC | 1 cut(s) | 179 |
| Eco47I | GGWCC | 2 cut(s) | 444, 1645 |
| Eco57I | CTGAAG | 1 cut(s) | 75 |
| Eco88I | CYCGRG | 1 cut(s) | 269 |
| EcoRI | GAATTC | 2 cut(s) | 307, 1055 |
| EcoRV | GATATC | 1 cut(s) | 179 |
| EcoT14I | CCWWGG | 4 cut(s) | 673, 921, 1215, 1561 |
| EcoT22I | ATGCAT | 1 cut(s) | 641 |
| ErhI | CCWWGG | 4 cut(s) | 673, 921, 1215, 1561 |
| FaeI | CATG | 7 cut(s) | 162, 785, 838, 981, 1073, 1219, 1559 |
| FaqI | GGGAC | 2 cut(s) | 856, 1283 |
| FatI | CATG | 7 cut(s) | 158, 781, 834, 977, 1069, 1215, 1555 |
| FauI | CCCGC | 1 cut(s) | 739 |
| FblI | GTMKAC | 1 cut(s) | 616 |
| Fnu4HI | GCNGC | 4 cut(s) | 266, 434, 715, 997 |
| FokI | GGATG | 5 cut(s) | 377, 412, 423, 648, 1112 |
| Fsp4HI | GCNGC | 4 cut(s) | 266, 434, 715, 997 |
| FspBI | CTAG | 3 cut(s) | 420, 1010, 1205 |
| FspI | TGCGCA | 1 cut(s) | 1530 |
| GlaI | GCGC | 4 cut(s) | 140, 758, 767, 1530 |
| GluI | GCNGC | 4 cut(s) | 266, 434, 715, 997 |
| HaeIII | GGCC | 2 cut(s) | 1428, 1560 |
| HapII | CCGG | 3 cut(s) | 21, 66, 442 |
| HhaI | GCGC | 4 cut(s) | 141, 759, 768, 1531 |
| Hin1II | CATG | 7 cut(s) | 162, 785, 838, 981, 1073, 1219, 1559 |
| Hin6I | GCGC | 4 cut(s) | 139, 757, 766, 1529 |
| HinP1I | GCGC | 4 cut(s) | 139, 757, 766, 1529 |
| HindIII | AAGCTT | 2 cut(s) | 425, 1190 |
| HinfI | GANTC | 7 cut(s) | 240, 466, 1039, 1090, 1296, 1403, 1694 |
| HpaII | CCGG | 3 cut(s) | 21, 66, 442 |
| HphI | GGTGA | 5 cut(s) | 33, 356, 619, 833, 1412 |
| Hpy166II | GTNNAC | 6 cut(s) | 447, 617, 1330, 1513, 1521, 1615 |
| Hpy188I | TCNGA | 5 cut(s) | 35, 313, 654, 1089, 1237 |
| Hpy188III | TCNNGA | 6 cut(s) | 221, 486, 800, 830, 1076, 1633 |
| Hpy8I | GTNNAC | 6 cut(s) | 447, 617, 1330, 1513, 1521, 1615 |
| Hpy99I | CGWCG | 1 cut(s) | 304 |
| HpyAV | CCTTC | 4 cut(s) | 268, 893, 1210, 1214 |
| HpyCH4III | ACNGT | 4 cut(s) | 479, 790, 1473, 1612 |
| HpyCH4IV | ACGT | 3 cut(s) | 302, 588, 732 |
| HpyCH4V | TGCA | 7 cut(s) | 123, 280, 403, 436, 639, 1450, 1550 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 120, 433, 763, 765, 974 |
| HpyF3I | CTNAG | 4 cut(s) | 11, 32, 450, 697 |
| HpySE526I | ACGT | 3 cut(s) | 302, 588, 732 |
| Hsp92II | CATG | 7 cut(s) | 162, 785, 838, 981, 1073, 1219, 1559 |
| HspAI | GCGC | 4 cut(s) | 139, 757, 766, 1529 |
| Kzo9I | GATC | 3 cut(s) | 773, 902, 1369 |
| LmnI | GCTCC | 2 cut(s) | 89, 1241 |
| Lsp1109I | GCAGC | 4 cut(s) | 252, 420, 701, 983 |
| LweI | GCATC | 8 cut(s) | 256, 390, 445, 626, 1277, 1459, 1540, 1629 |
| MaeI | CTAG | 3 cut(s) | 420, 1010, 1205 |
| MaeII | ACGT | 3 cut(s) | 302, 588, 732 |
| MaeIII | GTNAC | 2 cut(s) | 133, 916 |
| MalI | GATC | 3 cut(s) | 775, 904, 1371 |
| MboI | GATC | 3 cut(s) | 773, 902, 1369 |
| MboII | GAAGA | 5 cut(s) | 249, 361, 380, 1648, 1670 |
| MfeI | CAATTG | 1 cut(s) | 1689 |
| MflI | RGATCY | 1 cut(s) | 902 |
| MlsI | TGGCCA | 1 cut(s) | 1428 |
| MluCI | AATT | 9 cut(s) | 61, 190, 307, 481, 912, 1055, 1128, 1497, 1689 |
| MluNI | TGGCCA | 1 cut(s) | 1428 |
| MlyI | GAGTC | 1 cut(s) | 1305 |
| MmeI | TCCRAC | 1 cut(s) | 1399 |
| Mox20I | TGGCCA | 1 cut(s) | 1428 |
| Mph1103I | ATGCAT | 1 cut(s) | 641 |
| MroXI | GAANNNNTTC | 2 cut(s) | 191, 733 |
| MscI | TGGCCA | 1 cut(s) | 1428 |
| MseI | TTAA | 3 cut(s) | 60, 471, 1455 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1098, 1455 |
| Msp20I | TGGCCA | 1 cut(s) | 1428 |
| MspA1I | CMGCKG | 1 cut(s) | 1704 |
| MspI | CCGG | 3 cut(s) | 21, 66, 442 |
| MspR9I | CCNGG | 1 cut(s) | 442 |
| MunI | CAATTG | 1 cut(s) | 1689 |
| Mva1269I | GAATGC | 2 cut(s) | 292, 1274 |
| MvnI | CGCG | 2 cut(s) | 139, 1290 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 120, 433, 763, 765, 974 |
| NciI | CCSGG | 1 cut(s) | 442 |
| NcoI | CCATGG | 1 cut(s) | 1215 |
| NdeII | GATC | 3 cut(s) | 773, 902, 1369 |
| NlaIII | CATG | 7 cut(s) | 162, 785, 838, 981, 1073, 1219, 1559 |
| NlaIV | GGNNCC | 2 cut(s) | 787, 1647 |
| NmuCI | GTSAC | 1 cut(s) | 916 |
| NsbI | TGCGCA | 1 cut(s) | 1530 |
| NsiI | ATGCAT | 1 cut(s) | 641 |
| NspI | RCATGY | 3 cut(s) | 162, 838, 981 |
| PaeI | GCATGC | 1 cut(s) | 981 |
| PciI | ACATGT | 1 cut(s) | 158 |
| PctI | GAATGC | 2 cut(s) | 292, 1274 |
| PdmI | GAANNNNTTC | 2 cut(s) | 191, 733 |
| PfeI | GAWTC | 6 cut(s) | 240, 466, 1039, 1090, 1403, 1694 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1075 |
| PinAI | ACCGGT | 1 cut(s) | 20 |
| PkrI | GCNGC | 4 cut(s) | 267, 435, 716, 998 |
| PleI | GAGTC | 1 cut(s) | 1304 |
| PpsI | GAGTC | 1 cut(s) | 1304 |
| PscI | ACATGT | 1 cut(s) | 158 |
| Psp1406I | AACGTT | 1 cut(s) | 732 |
| PspN4I | GGNNCC | 2 cut(s) | 787, 1647 |
| PspPI | GGNCC | 2 cut(s) | 444, 1645 |
| PsuI | RGATCY | 1 cut(s) | 902 |
| PvuII | CAGCTG | 1 cut(s) | 1704 |
| RsaI | GTAC | 3 cut(s) | 410, 792, 1367 |
| RsaNI | GTAC | 3 cut(s) | 409, 791, 1366 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1098, 1455 |
| SaqAI | TTAA | 3 cut(s) | 60, 471, 1455 |
| SatI | GCNGC | 4 cut(s) | 266, 434, 715, 997 |
| Sau3AI | GATC | 3 cut(s) | 773, 902, 1369 |
| Sau96I | GGNCC | 2 cut(s) | 444, 1645 |
| SchI | GAGTC | 1 cut(s) | 1305 |
| ScrFI | CCNGG | 1 cut(s) | 442 |
| SfaNI | GCATC | 8 cut(s) | 256, 390, 445, 626, 1277, 1459, 1540, 1629 |
| SfcI | CTRYAG | 2 cut(s) | 1245, 1591 |
| SinI | GGWCC | 2 cut(s) | 444, 1645 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1098, 1455 |
| SmlI | CTYRAG | 2 cut(s) | 221, 507 |
| SmoI | CTYRAG | 2 cut(s) | 221, 507 |
| SphI | GCATGC | 1 cut(s) | 981 |
| Sse9I | AATT | 9 cut(s) | 61, 190, 307, 481, 912, 1055, 1128, 1497, 1689 |
| SsiI | CCGC | 4 cut(s) | 87, 534, 746, 1682 |
| SspMI | CTAG | 3 cut(s) | 420, 1010, 1205 |
| StyD4I | CCNGG | 1 cut(s) | 440 |
| StyI | CCWWGG | 4 cut(s) | 673, 921, 1215, 1561 |
| TaaI | ACNGT | 4 cut(s) | 479, 790, 1473, 1612 |
| TaiI | ACGT | 3 cut(s) | 305, 591, 735 |
| TaqI | TCGA | 2 cut(s) | 250, 299 |
| TasI | AATT | 9 cut(s) | 61, 190, 307, 481, 912, 1055, 1128, 1497, 1689 |
| TatI | WGTACW | 1 cut(s) | 408 |
| TfiI | GAWTC | 6 cut(s) | 240, 466, 1039, 1090, 1403, 1694 |
| Tru1I | TTAA | 3 cut(s) | 60, 471, 1455 |
| Tru9I | TTAA | 3 cut(s) | 60, 471, 1455 |
| TscAI | CASTG | 4 cut(s) | 454, 696, 963, 1171 |
| TseFI | GTSAC | 1 cut(s) | 916 |
| TseI | GCWGC | 4 cut(s) | 265, 433, 714, 996 |
| Tsp45I | GTSAC | 1 cut(s) | 916 |
| TspDTI | ATGAA | 9 cut(s) | 381, 570, 596, 770, 877, 1185, 1328, 1395, 1586 |
| TspGWI | ACGGA | 1 cut(s) | 865 |
| TspRI | CASTG | 4 cut(s) | 454, 696, 963, 1171 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1075 |
| VpaK11BI | GGWCC | 2 cut(s) | 444, 1645 |
| XapI | RAATTY | 3 cut(s) | 190, 307, 1055 |
| XceI | RCATGY | 3 cut(s) | 162, 838, 981 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1436 |
| XmiI | GTMKAC | 1 cut(s) | 616 |
| XmnI | GAANNNNTTC | 2 cut(s) | 191, 733 |
| XspI | CTAG | 3 cut(s) | 420, 1010, 1205 |
| Zsp2I | ATGCAT | 1 cut(s) | 641 |