MD04G1236800.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001268
Physical Location & Seq
Forward (+)
31545560 .. 31547854
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1236800.v1.1.491

Sequence Viewer

Length: 2295 bp
ATGGGGCATAACAATGGGGTTCCTCTTCCCTTCATTTTCTCTCTTGTTGCATTGTTTTTGTTGGCTCTGCATGTCAAGGTGTCTTTCGCAGATAGGTCGACGTATATTGTCCACATGGACAAGTCCCTCATGCCCAAGTCCTACTCTAGCCATGGCCATTGGTATTCCTCCATTGTTGATTCATTCAAATCCGGAAACCCTACCTCATTCGATGGCAACAAAATCTTGCCTTCCATTCTGTACACCTATGACAATGCCTTTCATGGGTTCAGTGCAGTTCTATCTGCTGATGAATTAGTGACTCTAAAAAAGTCACCGGGTTTCATCTCAGCTTACGCTGACAAGTCCATTACGCTCGACACCACCCACACCACTCAGTTCCTCAACCTCAATCCTTTCGCCGGGCTGTGGCCTGCTTCAGATTACGGAGATGACATCATCATTGGTGTCATTGATACCGGTCTCTGGCCCGAGAGTGAAAGCTTCAGAGATGAAGGTATGACCAAAAGTCTTCCAGCTAGGTGGAAAGGAAAATGCGAGGTTGGACAAGAGTTCAATGCCTCTCTTTGTAACTATAAATTGATTGGAGCTCGATACTTTAATAAGGGTGTCATGGCTGCAAACCCCGATGTCACACTTAGCATGAACTCGGCTAGAGACAGTTTAGGGCATGGGACGCATACATCTTCCACAGCTGCGGGGAACTACGTAGACGGAGCATCTTATTTTGGTTACGCTAAAGGAACAGCCAGAGGCGTAGCACCGCGTGCCAGAGTTGCCATGTACAAGGTTAGCTGGGATGAGGGGCGTTATGCCTCTGATGTTCTAGCTGGTATGGACCAAGCTATTGCTGATGGTGTTGATGTTATTTCAATTTCCTCTGGCTTTGACGACACACCATTGTACGAGGATCCCGTAGCGATTGCTTCATTTGCAGCCATGGAGAAGGGTGTGGTGGTTTCAGCTTCAGCCGGAAATGAAGGCCCCGAGCTTGTGACATTGCACAATGGCATCCCTTGGGTCTTGACCGTTGCAGCCGGCACAATAGACCGCTCATTTGGAGGAGCATTAACCTTTGGTAATGGTTTAACCATTACTGGATTTACCTTGTTTCCAGCAAACTCCTTAGTCGAAAACTCGCCACTGGTTTACAACGAGACATTCTCAGCATGCAACTCAACCGCAGCGTTAGAAACTGCCCCAGATGCGATCATCATATGTGATGACACGGTGCCTATTCGTAATCAAATATCTTACATCATTCAATCGGGGCTTCTTGGAGCTATCTTTATTACCAATAATCCTGAGATACGCGAATTAGGTTACGTTGTAACTCCTAGCGTTGTGGTGAATACGAAGGATGGGCGGACTGTGATCGAGTACGCACTAAAAAGTGAAAACCCTACTGTCAGCATAAATTTCCAACAAACGTTGCTCAATACAAAGCCTGCCCCCGCTGCTGCTTTTTACACTTCGAGAGGTCCTTCAAAAAGTTATCCGGGCATTTTGAAGCCGGACATAATGGCCCCGGGGTCATTAGTTTTAGCTGCTTGGGTTCCTAAGGTTGCAGCAGGTAAAATCGGGTTCAATGTGAACTTACCTAGCAACTACAATCTGATATCCGGGACATCCATGGCATGCCCTCATGCTTCAGGTGTAGCTGCTCTACTGAAAGGTGCGCACCCAGAATGGAGTGCAGCAGCAATTAGATCTGCTTTGATGACCACAGCTAACCCATTGGACAATACCGGCAATCCAATTCTTGACGACGGTGACAATTTCAACTTTGCTTCACCATTAGCTATGGGAGCAGGTCACATCGATCCTAATGGAGCACTTGATCCGGGCCTAATATACGATGCAACTCCACAAGAATATGTCAATCTCTTATGCTCCACCAACTTTACGCGTAACCAAATCTTATCCATCACTAGATCACACGCCTATGATTGTTCCAAACCATCTTCTGATCTCAACTATCCATCTTTCATCGCGTTGTACAACAATCATCACAAAACAAAGAAAAGGGTTCAAACATTTCAGAGGACCGTAACGAATGTGGGAGATGGCGCGGCTAGGTACAGGGCTTCGGTGACTGCTCCAATGGGTTCTCAGGTAACAGTCTCTCCAGAGATACTGATCTTTGCATACAAGTATGAGAAGCAAAGTTTTACGGTCACTTTAAATTTCAATGCGAAGAAGAAAGGGAACGCTTCTTCTGGCGCACTTGTTTGGATCGAACAAAATGGGAAATACACTGTCAGGAGCCCAATTGTAGTGTCACCTCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

765

Amino Acids

81.79

Weight (kDa)

6.16

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 119 2.9e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 143 - 581 4.5e-45 Subtilase family
fn3_6 PF17766 657 - 760 2.9e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1680
Acc36I ACCTGC 2 cut(s) 1562, 1802
AccB1I GGYRCC 1 cut(s) 1231
AccBSI CCGCTC 1 cut(s) 1053
AccI GTMKAC 2 cut(s) 98, 711
AccII CGCG 5 cut(s) 766, 1314, 1909, 1994, 2072
AccIII TCCGGA 1 cut(s) 191
AciI CCGC 7 cut(s) 698, 764, 1051, 1182, 1366, 1455, 2072
AclI AACGTT 1 cut(s) 1430
AclWI GGATC 5 cut(s) 905, 918, 1817, 1835, 2243
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 2 cut(s) 1417, 2185
AcuI CTGAAG 4 cut(s) 402, 469, 951, 1635
AdeI CACNNNGTG 1 cut(s) 767
AfaI GTAC 6 cut(s) 242, 785, 905, 1382, 2000, 2081
AfiI CCNNNNNNNGG 4 cut(s) 264, 401, 408, 465
AflIII ACRYGT 1 cut(s) 1907
AgeI ACCGGT 1 cut(s) 458
AhdI GACNNNNNGTC 1 cut(s) 507
Alw21I GWGCWC 2 cut(s) 592, 1837
Alw26I GTCTC 4 cut(s) 467, 651, 1151, 2128
AlwI GGATC 5 cut(s) 905, 918, 1817, 1835, 2243
Ama87I CYCGRG 3 cut(s) 470, 986, 1528
Aor13HI TCCGGA 1 cut(s) 191
AoxI GGCC 6 cut(s) 154, 410, 467, 982, 1524, 1846
ApoI RAATTY 2 cut(s) 1417, 2185
ArsI GACNNNNNNTTYG 2 cut(s) 1040, 1072
AsiGI ACCGGT 1 cut(s) 458
AspLEI GCGC 3 cut(s) 1681, 2072, 2225
AspS9I GGNCC 7 cut(s) 468, 838, 983, 1481, 1525, 1846, 2046
AsuC2I CCSGG 7 cut(s) 318, 403, 1500, 1529, 1530, 1624, 1845
AsuHPI GGTGA 6 cut(s) 306, 1360, 1784, 1785, 2104, 2274
AvaI CYCGRG 3 cut(s) 470, 986, 1528
AvaII GGWCC 3 cut(s) 838, 1481, 2046
AxyI CCTNAGG 1 cut(s) 1560
BalI TGGCCA 1 cut(s) 156
BamHI GGATCC 1 cut(s) 910
BanI GGYRCC 1 cut(s) 1231
BanII GRGCYC 2 cut(s) 592, 2270
BbsI GAAGAC 1 cut(s) 503
Bbv12I GWGCWC 2 cut(s) 592, 1837
BccI CCATC 7 cut(s) 206, 848, 1355, 1934, 1969, 1990, 2060
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BcnI CCSGG 7 cut(s) 318, 403, 1500, 1529, 1530, 1624, 1845
BcoDI GTCTC 4 cut(s) 467, 651, 1151, 2128
BfaI CTAG 8 cut(s) 147, 519, 654, 827, 1338, 1602, 1932, 2076
BfuAI ACCTGC 2 cut(s) 1562, 1802
BglII AGATCT 1 cut(s) 1709
Bme1390I CCNGG 7 cut(s) 318, 403, 1500, 1529, 1530, 1624, 1845
Bme18I GGWCC 3 cut(s) 838, 1481, 2046
BmeRI GACNNNNNGTC 1 cut(s) 507
BmeT110I CYCGRG 3 cut(s) 470, 986, 1528
BmgT120I GGNCC 7 cut(s) 468, 838, 983, 1481, 1525, 1846, 2046
BmiI GGNNCC 7 cut(s) 21, 912, 985, 1233, 1527, 1557, 2267
BmrFI CCNGG 7 cut(s) 318, 403, 1500, 1529, 1530, 1624, 1845
BmsI GCATC 4 cut(s) 728, 1020, 1195, 1849
BpiI GAAGAC 1 cut(s) 503
BplI GAGNNNNNCTC 2 cut(s) 1148, 1180
BpmI CTGGAG 1 cut(s) 2112
BpuMI CCSGG 7 cut(s) 318, 403, 1500, 1529, 1530, 1624, 1845
Bsa29I ATCGAT 1 cut(s) 1821
BsaAI YACGTR 1 cut(s) 709
BsaBI GATNNNNATC 1 cut(s) 2138
BsaI GGTCTC 1 cut(s) 467
BsaJI CCNNGG 7 cut(s) 151, 939, 1016, 1527, 1528, 1529, 1632
BsaWI WCCGGW 2 cut(s) 191, 458
BsaXI ACNNNNNCTCC 2 cut(s) 2110, 2140
Bsc4I CCNNNNNNNGG 4 cut(s) 264, 401, 408, 465
Bse118I RCCGGY 3 cut(s) 458, 1037, 1748
Bse1I ACTGG 2 cut(s) 1102, 1149
Bse21I CCTNAGG 1 cut(s) 1560
Bse3DI GCAATG 1 cut(s) 998
Bse8I GATNNNNATC 1 cut(s) 2138
BseAI TCCGGA 1 cut(s) 191
BseCI ATCGAT 1 cut(s) 1821
BseDI CCNNGG 7 cut(s) 151, 939, 1016, 1527, 1528, 1529, 1632
BseGI GGATG 4 cut(s) 805, 1011, 1366, 1628
BseJI GATNNNNATC 1 cut(s) 2138
BseLI CCNNNNNNNGG 4 cut(s) 264, 401, 408, 465
BseMI GCAATG 1 cut(s) 998
BseMII CTCAG 5 cut(s) 342, 389, 1179, 1296, 2126
BseNI ACTGG 2 cut(s) 1102, 1149
BseRI GAGGAG 1 cut(s) 1077
BseYI CCCAGC 1 cut(s) 795
BsgI GTGCAG 2 cut(s) 294, 1716
Bsh1236I CGCG 5 cut(s) 766, 1314, 1909, 1994, 2072
BshFI GGCC 6 cut(s) 156, 412, 469, 984, 1526, 1848
BshNI GGYRCC 1 cut(s) 1231
BshTI ACCGGT 1 cut(s) 458
BshVI ATCGAT 1 cut(s) 1821
BsiHKAI GWGCWC 2 cut(s) 592, 1837
BsiHKCI CYCGRG 3 cut(s) 470, 986, 1528
BslFI GGGAC 3 cut(s) 109, 688, 1639
BslI CCNNNNNNNGG 4 cut(s) 264, 401, 408, 465
BsmAI GTCTC 4 cut(s) 467, 651, 1151, 2128
BsmFI GGGAC 3 cut(s) 109, 688, 1639
BsnI GGCC 6 cut(s) 156, 412, 469, 984, 1526, 1848
Bso31I GGTCTC 1 cut(s) 467
BsoBI CYCGRG 3 cut(s) 470, 986, 1528
Bsp1286I GDGCHC 3 cut(s) 592, 1837, 2270
Bsp13I TCCGGA 1 cut(s) 191
Bsp1407I TGTACA 3 cut(s) 240, 783, 1998
Bsp19I CCATGG 3 cut(s) 151, 939, 1632
BspACI CCGC 7 cut(s) 698, 764, 1051, 1182, 1366, 1455, 2072
BspANI GGCC 6 cut(s) 156, 412, 469, 984, 1526, 1848
BspCNI CTCAG 5 cut(s) 341, 388, 1178, 1297, 2125
BspDI ATCGAT 1 cut(s) 1821
BspEI TCCGGA 1 cut(s) 191
BspFNI CGCG 5 cut(s) 766, 1314, 1909, 1994, 2072
BspLI GGNNCC 7 cut(s) 21, 912, 985, 1233, 1527, 1557, 2267
BspMI ACCTGC 2 cut(s) 1562, 1802
BspPI GGATC 5 cut(s) 905, 918, 1817, 1835, 2243
BspT107I GGYRCC 1 cut(s) 1231
BspTNI GGTCTC 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 1053
BsrDI GCAATG 1 cut(s) 998
BsrFI RCCGGY 3 cut(s) 458, 1037, 1748
BsrGI TGTACA 3 cut(s) 240, 783, 1998
BsrI ACTGG 2 cut(s) 1102, 1149
BssAI RCCGGY 3 cut(s) 458, 1037, 1748
BssECI CCNNGG 7 cut(s) 151, 939, 1016, 1527, 1528, 1529, 1632
BssT1I CCWWGG 4 cut(s) 151, 939, 1016, 1632
Bst6I CTCTTC 1 cut(s) 30
BstAPI GCANNNNNTGC 1 cut(s) 767
BstAUI TGTACA 3 cut(s) 240, 783, 1998
BstBAI YACGTR 1 cut(s) 709
BstC8I GCNNGC 6 cut(s) 414, 768, 1039, 1171, 1449, 1639
BstDEI CTNAG 8 cut(s) 328, 375, 638, 1126, 1165, 1305, 1560, 2112
BstDSI CCRYGG 3 cut(s) 151, 939, 1632
BstF5I GGATG 4 cut(s) 805, 1011, 1366, 1628
BstFNI CGCG 5 cut(s) 766, 1314, 1909, 1994, 2072
BstHHI GCGC 3 cut(s) 1681, 2072, 2225
BstMAI GTCTC 4 cut(s) 467, 651, 1151, 2128
BstMWI GCNNNNNNNGC 7 cut(s) 676, 767, 776, 932, 1205, 1457, 1808
BstNSI RCATGY 3 cut(s) 74, 1173, 1641
BstSCI CCNGG 7 cut(s) 316, 401, 1498, 1527, 1528, 1622, 1843
BstSNI TACGTA 1 cut(s) 709
BstUI CGCG 5 cut(s) 766, 1314, 1909, 1994, 2072
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 2 cut(s) 910, 1709
BstXI CCANNNNNNTGG 1 cut(s) 522
BstYI RGATCY 2 cut(s) 910, 1709
Bsu15I ATCGAT 1 cut(s) 1821
Bsu36I CCTNAGG 1 cut(s) 1560
BsuRI GGCC 6 cut(s) 156, 412, 469, 984, 1526, 1848
BsuTUI ATCGAT 1 cut(s) 1821
BtgI CCRYGG 3 cut(s) 151, 939, 1632
BtgZI GCGATG 1 cut(s) 1975
BtsCI GGATG 4 cut(s) 805, 1011, 1366, 1628
BtsIMutI CAGTG 3 cut(s) 277, 1142, 2256
BveI ACCTGC 2 cut(s) 1562, 1802
Cac8I GCNNGC 6 cut(s) 414, 768, 1039, 1171, 1449, 1639
CfoI GCGC 3 cut(s) 1681, 2072, 2225
Cfr10I RCCGGY 3 cut(s) 458, 1037, 1748
Cfr13I GGNCC 7 cut(s) 468, 838, 983, 1481, 1525, 1846, 2046
Cfr9I CCCGGG 1 cut(s) 1528
ClaI ATCGAT 1 cut(s) 1821
CseI GACGC 1 cut(s) 685
Csp6I GTAC 6 cut(s) 241, 784, 904, 1381, 1999, 2080
CspAI ACCGGT 1 cut(s) 458
CviQI GTAC 6 cut(s) 241, 784, 904, 1381, 1999, 2080
DdeI CTNAG 8 cut(s) 328, 375, 638, 1126, 1165, 1305, 1560, 2112
DraI TTTAAA 1 cut(s) 2184
DraIII CACNNNGTG 1 cut(s) 767
DriI GACNNNNNGTC 1 cut(s) 507
EaeI YGGCCR 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 30
Eam1105I GACNNNNNGTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 30
EciI GGCGGA 1 cut(s) 1381
Ecl136II GAGCTC 1 cut(s) 590
Eco105I TACGTA 1 cut(s) 709
Eco130I CCWWGG 4 cut(s) 151, 939, 1016, 1632
Eco24I GRGCYC 2 cut(s) 592, 2270
Eco31I GGTCTC 1 cut(s) 467
Eco32I GATATC 1 cut(s) 1620
Eco47I GGWCC 3 cut(s) 838, 1481, 2046
Eco53kI GAGCTC 1 cut(s) 590
Eco57I CTGAAG 4 cut(s) 402, 469, 951, 1635
Eco81I CCTNAGG 1 cut(s) 1560
Eco88I CYCGRG 3 cut(s) 470, 986, 1528
EcoICRI GAGCTC 1 cut(s) 590
EcoO109I RGGNCCY 2 cut(s) 983, 1481
EcoRV GATATC 1 cut(s) 1620
EcoT14I CCWWGG 4 cut(s) 151, 939, 1016, 1632
EcoT38I GRGCYC 2 cut(s) 592, 2270
ErhI CCWWGG 4 cut(s) 151, 939, 1016, 1632
FaqI GGGAC 3 cut(s) 109, 688, 1639
FauI CCCGC 2 cut(s) 691, 1462
FauNDI CATATG 1 cut(s) 1217
FblI GTMKAC 2 cut(s) 98, 711
FokI GGATG 4 cut(s) 812, 998, 1373, 1615
FriOI GRGCYC 2 cut(s) 592, 2270
FspAI RTGCGCAY 1 cut(s) 1680
FspBI CTAG 8 cut(s) 147, 519, 654, 827, 1338, 1602, 1932, 2076
FspI TGCGCA 1 cut(s) 1680
GlaI GCGC 3 cut(s) 1680, 2071, 2224
GsaI CCCAGC 1 cut(s) 799
GsuI CTGGAG 1 cut(s) 2112
HaeIII GGCC 6 cut(s) 156, 412, 469, 984, 1526, 1848
HgaI GACGC 1 cut(s) 685
HhaI GCGC 3 cut(s) 1681, 2072, 2225
Hin6I GCGC 3 cut(s) 1679, 2070, 2223
HinP1I GCGC 3 cut(s) 1679, 2070, 2223
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HindIII AAGCTT 1 cut(s) 481
HinfI GANTC 2 cut(s) 179, 301
HphI GGTGA 6 cut(s) 306, 1360, 1784, 1785, 2104, 2274
Hpy166II GTNNAC 6 cut(s) 99, 112, 243, 712, 1150, 1594
Hpy188I TCNGA 6 cut(s) 421, 488, 820, 1617, 1969, 2043
Hpy188III TCNNGA 7 cut(s) 192, 1024, 1304, 1476, 1763, 2129, 2263
Hpy8I GTNNAC 6 cut(s) 99, 112, 243, 712, 1150, 1594
Hpy99I CGWCG 2 cut(s) 103, 1772
HpyAV CCTTC 7 cut(s) 40, 240, 488, 940, 974, 1351, 1494
HpyCH4IV ACGT 4 cut(s) 101, 708, 1326, 1430
HpyF10VI GCNNNNNNNGC 7 cut(s) 676, 767, 776, 932, 1205, 1457, 1808
HpyF3I CTNAG 8 cut(s) 328, 375, 638, 1126, 1165, 1305, 1560, 2112
HpySE526I ACGT 4 cut(s) 101, 708, 1326, 1430
HspAI GCGC 3 cut(s) 1679, 2070, 2223
Kpn2I TCCGGA 1 cut(s) 191
KroI GCCGGC 1 cut(s) 1037
KroNI GCCGGC 1 cut(s) 1039
LmnI GCTCC 9 cut(s) 587, 716, 1064, 1280, 1808, 1832, 1898, 2104, 2265
LweI GCATC 4 cut(s) 728, 1020, 1195, 1849
MaeI CTAG 8 cut(s) 147, 519, 654, 827, 1338, 1602, 1932, 2076
MaeII ACGT 4 cut(s) 101, 708, 1326, 1430
MbiI CCGCTC 1 cut(s) 1053
MboII GAAGA 7 cut(s) 17, 503, 678, 1956, 2208, 2209, 2212
MfeI CAATTG 1 cut(s) 2271
MflI RGATCY 2 cut(s) 910, 1709
MhlI GDGCHC 3 cut(s) 592, 1837, 2270
MlsI TGGCCA 1 cut(s) 156
MluI ACGCGT 1 cut(s) 1907
MluNI TGGCCA 1 cut(s) 156
MlyI GAGTC 1 cut(s) 295
MmeI TCCRAC 2 cut(s) 523, 1447
Mox20I TGGCCA 1 cut(s) 156
MroI TCCGGA 1 cut(s) 191
MroNI GCCGGC 1 cut(s) 1037
MscI TGGCCA 1 cut(s) 156
MseI TTAA 4 cut(s) 600, 1070, 1088, 2183
MslI CAYNNNNRTG 2 cut(s) 12, 1944
Msp20I TGGCCA 1 cut(s) 156
MspA1I CMGCKG 2 cut(s) 695, 1457
MspR9I CCNGG 7 cut(s) 318, 403, 1500, 1529, 1530, 1624, 1845
MunI CAATTG 1 cut(s) 2271
MvnI CGCG 5 cut(s) 766, 1314, 1909, 1994, 2072
MwoI GCNNNNNNNGC 7 cut(s) 676, 767, 776, 932, 1205, 1457, 1808
NaeI GCCGGC 1 cut(s) 1039
NciI CCSGG 7 cut(s) 318, 403, 1500, 1529, 1530, 1624, 1845
NcoI CCATGG 3 cut(s) 151, 939, 1632
NdeI CATATG 1 cut(s) 1217
NgoMIV GCCGGC 1 cut(s) 1037
NlaIV GGNNCC 7 cut(s) 21, 912, 985, 1233, 1527, 1557, 2267
NmeAIII GCCGAG 1 cut(s) 629
NmuCI GTSAC 9 cut(s) 298, 312, 631, 994, 1772, 1814, 2092, 2176, 2280
NsbI TGCGCA 1 cut(s) 1680
NspI RCATGY 3 cut(s) 74, 1173, 1641
PaeI GCATGC 2 cut(s) 1173, 1641
PcsI WCGNNNNNNNCGW 1 cut(s) 912
PdiI GCCGGC 1 cut(s) 1039
PfeI GAWTC 1 cut(s) 179
PfoI TCCNGGA 1 cut(s) 1622
PinAI ACCGGT 1 cut(s) 458
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Ppu21I YACGTR 1 cut(s) 709
PpuMI RGGWCCY 1 cut(s) 1481
Psp124BI GAGCTC 1 cut(s) 592
Psp1406I AACGTT 1 cut(s) 1430
Psp5II RGGWCCY 1 cut(s) 1481
PspFI CCCAGC 1 cut(s) 795
PspN4I GGNNCC 7 cut(s) 21, 912, 985, 1233, 1527, 1557, 2267
PspPI GGNCC 7 cut(s) 468, 838, 983, 1481, 1525, 1846, 2046
PspPPI RGGWCCY 1 cut(s) 1481
PsuI RGATCY 2 cut(s) 910, 1709
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 6 cut(s) 242, 785, 905, 1382, 2000, 2081
RsaNI GTAC 6 cut(s) 241, 784, 904, 1381, 1999, 2080
RseI CAYNNNNRTG 2 cut(s) 12, 1944
SacI GAGCTC 1 cut(s) 592
SalI GTCGAC 1 cut(s) 97
SaqAI TTAA 4 cut(s) 600, 1070, 1088, 2183
Sau96I GGNCC 7 cut(s) 468, 838, 983, 1481, 1525, 1846, 2046
SchI GAGTC 1 cut(s) 295
ScrFI CCNGG 7 cut(s) 318, 403, 1500, 1529, 1530, 1624, 1845
SduI GDGCHC 3 cut(s) 592, 1837, 2270
SfaNI GCATC 4 cut(s) 728, 1020, 1195, 1849
SinI GGWCC 3 cut(s) 838, 1481, 2046
SmaI CCCGGG 1 cut(s) 1530
SmiMI CAYNNNNRTG 2 cut(s) 12, 1944
SnaBI TACGTA 1 cut(s) 709
SphI GCATGC 2 cut(s) 1173, 1641
SsiI CCGC 7 cut(s) 698, 764, 1051, 1182, 1366, 1455, 2072
SspMI CTAG 8 cut(s) 147, 519, 654, 827, 1338, 1602, 1932, 2076
SstI GAGCTC 1 cut(s) 592
StyD4I CCNGG 7 cut(s) 316, 401, 1498, 1527, 1528, 1622, 1843
StyI CCWWGG 4 cut(s) 151, 939, 1016, 1632
TaiI ACGT 4 cut(s) 104, 711, 1329, 1433
TaqI TCGA 9 cut(s) 98, 210, 357, 592, 1131, 1377, 1475, 1821, 2238
TatI WGTACW 3 cut(s) 240, 783, 1998
TauI GCSGC 1 cut(s) 2075
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 4 cut(s) 600, 1070, 1088, 2183
Tru9I TTAA 4 cut(s) 600, 1070, 1088, 2183
TscAI CASTG 3 cut(s) 277, 1149, 2263
TseFI GTSAC 9 cut(s) 298, 312, 631, 994, 1772, 1814, 2092, 2176, 2280
Tsp45I GTSAC 9 cut(s) 298, 312, 631, 994, 1772, 1814, 2092, 2176, 2280
TspGWI ACGGA 2 cut(s) 441, 729
TspMI CCCGGG 1 cut(s) 1528
TspRI CASTG 3 cut(s) 277, 1149, 2263
VpaK11BI GGWCC 3 cut(s) 838, 1481, 2046
XapI RAATTY 2 cut(s) 1417, 2185
XceI RCATGY 3 cut(s) 74, 1173, 1641
XmaI CCCGGG 1 cut(s) 1528
XmiI GTMKAC 2 cut(s) 98, 711
XspI CTAG 8 cut(s) 147, 519, 654, 827, 1338, 1602, 1932, 2076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.