subtilisin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 349 |
| Acc36I | ACCTGC | 2 cut(s) | 330, 1733 |
| Acc65I | GGTACC | 1 cut(s) | 413 |
| AccB1I | GGYRCC | 2 cut(s) | 413, 736 |
| AccI | GTMKAC | 1 cut(s) | 2012 |
| AccII | CGCG | 2 cut(s) | 336, 1793 |
| AciI | CCGC | 4 cut(s) | 243, 672, 729, 1029 |
| AcoI | YGGCCR | 7 cut(s) | 389, 446, 669, 687, 753, 1731, 2214 |
| AcsI | RAATTY | 1 cut(s) | 1538 |
| AcuI | CTGAAG | 2 cut(s) | 294, 2202 |
| AcvI | CACGTG | 1 cut(s) | 751 |
| AcyI | GRCGYC | 2 cut(s) | 737, 888 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 297, 444, 929, 1035, 1292, 1774, 1984, 2170, 2212 |
| AflII | CTTAAG | 1 cut(s) | 238 |
| AgsI | TTSAA | 8 cut(s) | 998, 1071, 1114, 1193, 1355, 1543, 1933, 1947 |
| AhlI | ACTAGT | 1 cut(s) | 1394 |
| AjnI | CCWGG | 3 cut(s) | 295, 1152, 1668 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 596, 628, 1328, 1360 |
| Alw21I | GWGCWC | 2 cut(s) | 1612, 1828 |
| Alw26I | GTCTC | 5 cut(s) | 79, 263, 312, 1369, 2138 |
| Alw44I | GTGCAC | 2 cut(s) | 1608, 1824 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1528 |
| Ama87I | CYCGRG | 2 cut(s) | 741, 1774 |
| ApaLI | GTGCAC | 2 cut(s) | 1608, 1824 |
| ApeKI | GCWGC | 6 cut(s) | 590, 971, 1478, 1580, 1592, 1631 |
| ApoI | RAATTY | 1 cut(s) | 1538 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1509, 1541 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1495 |
| Asp718I | GGTACC | 1 cut(s) | 413 |
| AspLEI | GCGC | 4 cut(s) | 715, 739, 838, 1597 |
| AspS9I | GGNCC | 5 cut(s) | 847, 920, 1412, 1525, 1765 |
| AsuC2I | CCSGG | 7 cut(s) | 439, 691, 717, 1053, 1461, 1775, 1776 |
| AsuHPI | GGTGA | 7 cut(s) | 143, 607, 1307, 1715, 1852, 1999, 2209 |
| AvaI | CYCGRG | 2 cut(s) | 741, 1774 |
| AvaII | GGWCC | 4 cut(s) | 847, 920, 1412, 1525 |
| BaeGI | GKGCMC | 3 cut(s) | 1574, 1612, 1828 |
| BalI | TGGCCA | 4 cut(s) | 448, 755, 1733, 2216 |
| BanI | GGYRCC | 2 cut(s) | 413, 736 |
| BanII | GRGCYC | 1 cut(s) | 748 |
| BbrPI | CACGTG | 1 cut(s) | 751 |
| BbsI | GAAGAC | 3 cut(s) | 883, 1059, 2137 |
| Bbv12I | GWGCWC | 2 cut(s) | 1612, 1828 |
| BbvI | GCAGC | 6 cut(s) | 602, 983, 1465, 1579, 1592, 1643 |
| BccI | CCATC | 6 cut(s) | 24, 492, 821, 884, 1423, 1865 |
| BceAI | ACGGC | 2 cut(s) | 421, 684 |
| BciT130I | CCWGG | 3 cut(s) | 297, 1154, 1670 |
| BclI | TGATCA | 2 cut(s) | 148, 1705 |
| BcnI | CCSGG | 7 cut(s) | 439, 691, 717, 1053, 1461, 1775, 1776 |
| BcoDI | GTCTC | 5 cut(s) | 79, 263, 312, 1369, 2138 |
| BcuI | ACTAGT | 1 cut(s) | 1394 |
| BfaI | CTAG | 5 cut(s) | 975, 1395, 1407, 1722, 2257 |
| BfmI | CTRYAG | 2 cut(s) | 174, 1629 |
| BfoI | RGCGCY | 1 cut(s) | 740 |
| BfrI | CTTAAG | 1 cut(s) | 238 |
| BfuAI | ACCTGC | 2 cut(s) | 330, 1733 |
| BglII | AGATCT | 1 cut(s) | 1900 |
| BisI | GCNGC | 8 cut(s) | 591, 672, 730, 972, 1479, 1581, 1593, 1632 |
| BlpI | GCTNAGC | 1 cut(s) | 2102 |
| BlsI | GCNGC | 8 cut(s) | 592, 673, 731, 973, 1480, 1582, 1594, 1633 |
| Bme18I | GGWCC | 4 cut(s) | 847, 920, 1412, 1525 |
| BmeT110I | CYCGRG | 2 cut(s) | 741, 1774 |
| BmgT120I | GGNCC | 5 cut(s) | 847, 920, 1412, 1525, 1765 |
| BmiI | GGNNCC | 4 cut(s) | 415, 738, 849, 1687 |
| BmrI | ACTGGG | 1 cut(s) | 349 |
| BmsI | GCATC | 3 cut(s) | 704, 1107, 1186 |
| BmuI | ACTGGG | 1 cut(s) | 349 |
| BpiI | GAAGAC | 3 cut(s) | 883, 1059, 2137 |
| BpmI | CTGGAG | 3 cut(s) | 279, 296, 1238 |
| Bpu10I | CCTNAGC | 1 cut(s) | 270 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2102 |
| BpuEI | CTTGAG | 3 cut(s) | 1190, 1424, 1953 |
| BpuMI | CCSGG | 7 cut(s) | 439, 691, 717, 1053, 1461, 1775, 1776 |
| BsaAI | YACGTR | 2 cut(s) | 751, 1810 |
| BsaHI | GRCGYC | 2 cut(s) | 737, 888 |
| BsaI | GGTCTC | 2 cut(s) | 312, 1369 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 297, 444, 929, 1035, 1292, 1774, 1984, 2170, 2212 |
| Bse118I | RCCGGY | 3 cut(s) | 325, 391, 1717 |
| Bse1I | ACTGG | 1 cut(s) | 355 |
| Bse3DI | GCAATG | 2 cut(s) | 1920, 2019 |
| BseBI | CCWGG | 3 cut(s) | 297, 1154, 1670 |
| BseGI | GGATG | 7 cut(s) | 108, 805, 1044, 1559, 1612, 1665, 2239 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 297, 444, 929, 1035, 1292, 1774, 1984, 2170, 2212 |
| BseMI | GCAATG | 2 cut(s) | 1920, 2019 |
| BseMII | CTCAG | 4 cut(s) | 261, 885, 2093, 2239 |
| BseNI | ACTGG | 1 cut(s) | 355 |
| BseSI | GKGCMC | 3 cut(s) | 1574, 1612, 1828 |
| BseX3I | CGGCCG | 1 cut(s) | 669 |
| BseXI | GCAGC | 6 cut(s) | 602, 983, 1465, 1579, 1592, 1643 |
| BseYI | CCCAGC | 1 cut(s) | 1583 |
| BsgI | GTGCAG | 2 cut(s) | 990, 1440 |
| Bsh1236I | CGCG | 2 cut(s) | 336, 1793 |
| Bsh1285I | CGRYCG | 1 cut(s) | 672 |
| BshNI | GGYRCC | 2 cut(s) | 413, 736 |
| BsiEI | CGRYCG | 1 cut(s) | 672 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1612, 1828 |
| BsiHKCI | CYCGRG | 2 cut(s) | 741, 1774 |
| BslFI | GGGAC | 6 cut(s) | 337, 647, 664, 788, 1570, 1791 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 297, 444, 929, 1035, 1292, 1774, 1984, 2170, 2212 |
| BsmAI | GTCTC | 5 cut(s) | 79, 263, 312, 1369, 2138 |
| BsmBI | CGTCTC | 1 cut(s) | 2138 |
| BsmFI | GGGAC | 6 cut(s) | 337, 647, 664, 788, 1570, 1791 |
| Bso31I | GGTCTC | 2 cut(s) | 312, 1369 |
| BsoBI | CYCGRG | 2 cut(s) | 741, 1774 |
| Bsp1286I | GDGCHC | 4 cut(s) | 748, 1574, 1612, 1828 |
| Bsp1407I | TGTACA | 1 cut(s) | 759 |
| Bsp143I | GATC | 5 cut(s) | 148, 985, 1705, 1865, 1900 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2102 |
| Bsp19I | CCATGG | 2 cut(s) | 1563, 1734 |
| BspACI | CCGC | 4 cut(s) | 243, 672, 729, 1029 |
| BspCNI | CTCAG | 4 cut(s) | 262, 884, 2094, 2238 |
| BspFNI | CGCG | 2 cut(s) | 336, 1793 |
| BspHI | TCATGA | 1 cut(s) | 88 |
| BspLI | GGNNCC | 4 cut(s) | 415, 738, 849, 1687 |
| BspMAI | CTGCAG | 1 cut(s) | 1633 |
| BspMI | ACCTGC | 2 cut(s) | 330, 1733 |
| BspQI | GCTCTTC | 1 cut(s) | 541 |
| BspT107I | GGYRCC | 2 cut(s) | 413, 736 |
| BspTI | CTTAAG | 1 cut(s) | 238 |
| BspTNI | GGTCTC | 2 cut(s) | 312, 1369 |
| BsrDI | GCAATG | 2 cut(s) | 1920, 2019 |
| BsrFI | RCCGGY | 3 cut(s) | 325, 391, 1717 |
| BsrGI | TGTACA | 1 cut(s) | 759 |
| BsrI | ACTGG | 1 cut(s) | 355 |
| BssAI | RCCGGY | 3 cut(s) | 325, 391, 1717 |
| BssMI | GATC | 5 cut(s) | 148, 985, 1705, 1865, 1900 |
| BssNI | GRCGYC | 2 cut(s) | 737, 888 |
| BssT1I | CCWWGG | 5 cut(s) | 81, 953, 1563, 1734, 1839 |
| Bst2UI | CCWGG | 3 cut(s) | 297, 1154, 1670 |
| Bst4CI | ACNGT | 7 cut(s) | 175, 644, 794, 967, 1390, 1987, 2149 |
| Bst6I | CTCTTC | 3 cut(s) | 211, 541, 667 |
| BstACI | GRCGYC | 2 cut(s) | 737, 888 |
| BstAFI | CTTAAG | 1 cut(s) | 238 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1205 |
| BstAUI | TGTACA | 1 cut(s) | 759 |
| BstBAI | YACGTR | 2 cut(s) | 751, 1810 |
| BstC8I | GCNNGC | 3 cut(s) | 727, 938, 1570 |
| BstDEI | CTNAG | 5 cut(s) | 210, 270, 871, 2102, 2225 |
| BstDSI | CCRYGG | 2 cut(s) | 1563, 1734 |
| BstEII | GGTNACC | 2 cut(s) | 1987, 2159 |
| BstF5I | GGATG | 7 cut(s) | 108, 805, 1044, 1559, 1612, 1665, 2239 |
| BstFNI | CGCG | 2 cut(s) | 336, 1793 |
| BstH2I | RGCGCY | 1 cut(s) | 740 |
| BstHHI | GCGC | 4 cut(s) | 715, 739, 838, 1597 |
| BstKTI | GATC | 5 cut(s) | 151, 988, 1708, 1868, 1903 |
| BstMAI | GTCTC | 5 cut(s) | 79, 263, 312, 1369, 2138 |
| BstMBI | GATC | 5 cut(s) | 148, 985, 1705, 1865, 1900 |
| BstMCI | CGRYCG | 1 cut(s) | 672 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 743, 752, 1205, 1589, 1739 |
| BstNI | CCWGG | 3 cut(s) | 297, 1154, 1670 |
| BstNSI | RCATGY | 2 cut(s) | 513, 1580 |
| BstPI | GGTNACC | 2 cut(s) | 1987, 2159 |
| BstSFI | CTRYAG | 2 cut(s) | 174, 1629 |
| BstSLI | GKGCMC | 3 cut(s) | 1574, 1612, 1828 |
| BstSNI | TACGTA | 1 cut(s) | 1810 |
| BstUI | CGCG | 2 cut(s) | 336, 1793 |
| BstV1I | GCAGC | 6 cut(s) | 602, 983, 1465, 1579, 1592, 1643 |
| BstV2I | GAAGAC | 3 cut(s) | 883, 1059, 2137 |
| BstX2I | RGATCY | 1 cut(s) | 1900 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 362, 498, 733 |
| BstYI | RGATCY | 1 cut(s) | 1900 |
| BstZI | CGGCCG | 1 cut(s) | 669 |
| BtgI | CCRYGG | 2 cut(s) | 1563, 1734 |
| BtsCI | GGATG | 7 cut(s) | 108, 805, 1044, 1559, 1612, 1665, 2239 |
| BtsIMutI | CAGTG | 3 cut(s) | 171, 256, 2178 |
| BveI | ACCTGC | 2 cut(s) | 330, 1733 |
| Cac8I | GCNNGC | 3 cut(s) | 727, 938, 1570 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1528 |
| CciI | TCATGA | 1 cut(s) | 88 |
| CfoI | GCGC | 4 cut(s) | 715, 739, 838, 1597 |
| Cfr10I | RCCGGY | 3 cut(s) | 325, 391, 1717 |
| Cfr13I | GGNCC | 5 cut(s) | 847, 920, 1412, 1525, 1765 |
| Cfr9I | CCCGGG | 1 cut(s) | 1774 |
| CseI | GACGC | 4 cut(s) | 479, 661, 877, 1799 |
| DdeI | CTNAG | 5 cut(s) | 210, 270, 871, 2102, 2225 |
| DinI | GGCGCC | 1 cut(s) | 738 |
| DpnI | GATC | 5 cut(s) | 150, 987, 1707, 1867, 1902 |
| DpnII | GATC | 5 cut(s) | 148, 985, 1705, 1865, 1900 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 349 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 349 |
| EaeI | YGGCCR | 7 cut(s) | 389, 446, 669, 687, 753, 1731, 2214 |
| EagI | CGGCCG | 1 cut(s) | 669 |
| Eam1104I | CTCTTC | 3 cut(s) | 211, 541, 667 |
| EarI | CTCTTC | 3 cut(s) | 211, 541, 667 |
| EciI | GGCGGA | 1 cut(s) | 1018 |
| EclXI | CGGCCG | 1 cut(s) | 669 |
| Eco105I | TACGTA | 1 cut(s) | 1810 |
| Eco130I | CCWWGG | 5 cut(s) | 81, 953, 1563, 1734, 1839 |
| Eco147I | AGGCCT | 1 cut(s) | 281 |
| Eco24I | GRGCYC | 1 cut(s) | 748 |
| Eco31I | GGTCTC | 2 cut(s) | 312, 1369 |
| Eco47I | GGWCC | 4 cut(s) | 847, 920, 1412, 1525 |
| Eco52I | CGGCCG | 1 cut(s) | 669 |
| Eco57I | CTGAAG | 2 cut(s) | 294, 2202 |
| Eco72I | CACGTG | 1 cut(s) | 751 |
| Eco88I | CYCGRG | 2 cut(s) | 741, 1774 |
| Eco91I | GGTNACC | 2 cut(s) | 1987, 2159 |
| EcoO109I | RGGNCCY | 4 cut(s) | 847, 920, 1525, 1765 |
| EcoO65I | GGTNACC | 2 cut(s) | 1987, 2159 |
| EcoRI | GAATTC | 1 cut(s) | 1538 |
| EcoRII | CCWGG | 3 cut(s) | 295, 1152, 1668 |
| EcoT14I | CCWWGG | 5 cut(s) | 81, 953, 1563, 1734, 1839 |
| EcoT38I | GRGCYC | 1 cut(s) | 748 |
| EgeI | GGCGCC | 1 cut(s) | 738 |
| EheI | GGCGCC | 1 cut(s) | 738 |
| ErhI | CCWWGG | 5 cut(s) | 81, 953, 1563, 1734, 1839 |
| Esp3I | CGTCTC | 1 cut(s) | 2138 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 875, 907, 2090, 2122 |
| FaqI | GGGAC | 6 cut(s) | 337, 647, 664, 788, 1570, 1791 |
| FauI | CCCGC | 1 cut(s) | 236 |
| FauNDI | CATATG | 1 cut(s) | 2193 |
| FbaI | TGATCA | 2 cut(s) | 148, 1705 |
| FblI | GTMKAC | 1 cut(s) | 2012 |
| Fnu4HI | GCNGC | 8 cut(s) | 591, 672, 730, 972, 1479, 1581, 1593, 1632 |
| FokI | GGATG | 7 cut(s) | 95, 812, 1051, 1546, 1599, 1652, 2246 |
| FriOI | GRGCYC | 1 cut(s) | 748 |
| Fsp4HI | GCNGC | 8 cut(s) | 591, 672, 730, 972, 1479, 1581, 1593, 1632 |
| FspBI | CTAG | 5 cut(s) | 975, 1395, 1407, 1722, 2257 |
| GlaI | GCGC | 4 cut(s) | 714, 738, 837, 1596 |
| GluI | GCNGC | 8 cut(s) | 591, 672, 730, 972, 1479, 1581, 1593, 1632 |
| GsaI | CCCAGC | 1 cut(s) | 1587 |
| GsuI | CTGGAG | 3 cut(s) | 279, 296, 1238 |
| HaeII | RGCGCY | 1 cut(s) | 740 |
| HgaI | GACGC | 4 cut(s) | 479, 661, 877, 1799 |
| HhaI | GCGC | 4 cut(s) | 715, 739, 838, 1597 |
| Hin1I | GRCGYC | 2 cut(s) | 737, 888 |
| Hin6I | GCGC | 4 cut(s) | 713, 737, 836, 1595 |
| HinP1I | GCGC | 4 cut(s) | 713, 737, 836, 1595 |
| HincII | GTYRAC | 2 cut(s) | 2013, 2189 |
| HindII | GTYRAC | 2 cut(s) | 2013, 2189 |
| HindIII | AAGCTT | 2 cut(s) | 460, 1342 |
| HphI | GGTGA | 7 cut(s) | 143, 607, 1307, 1715, 1852, 1999, 2209 |
| Hpy188I | TCNGA | 6 cut(s) | 484, 821, 1663, 1979, 2084, 2233 |
| Hpy99I | CGWCG | 3 cut(s) | 644, 1390, 1663 |
| HpyAV | CCTTC | 8 cut(s) | 877, 911, 933, 1286, 1923, 1974, 2079, 2177 |
| HpyCH4III | ACNGT | 7 cut(s) | 175, 644, 794, 967, 1390, 1987, 2149 |
| HpyCH4IV | ACGT | 3 cut(s) | 750, 1658, 1809 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 743, 752, 1205, 1589, 1739 |
| HpyF3I | CTNAG | 5 cut(s) | 210, 270, 871, 2102, 2225 |
| HpySE526I | ACGT | 3 cut(s) | 750, 1658, 1809 |
| Hsp92I | GRCGYC | 2 cut(s) | 737, 888 |
| HspAI | GCGC | 4 cut(s) | 713, 737, 836, 1595 |
| KasI | GGCGCC | 1 cut(s) | 736 |
| KpnI | GGTACC | 1 cut(s) | 417 |
| Ksp22I | TGATCA | 2 cut(s) | 148, 1705 |
| Kzo9I | GATC | 5 cut(s) | 148, 985, 1705, 1865, 1900 |
| LguI | GCTCTTC | 1 cut(s) | 541 |
| LmnI | GCTCC | 3 cut(s) | 383, 506, 1739 |
| Lsp1109I | GCAGC | 6 cut(s) | 602, 983, 1465, 1579, 1592, 1643 |
| LweI | GCATC | 3 cut(s) | 704, 1107, 1186 |
| MaeI | CTAG | 5 cut(s) | 975, 1395, 1407, 1722, 2257 |
| MaeII | ACGT | 3 cut(s) | 750, 1658, 1809 |
| MaeIII | GTNAC | 9 cut(s) | 49, 607, 989, 1103, 1390, 1397, 1987, 2029, 2159 |
| MalI | GATC | 5 cut(s) | 150, 987, 1707, 1867, 1902 |
| MboI | GATC | 5 cut(s) | 148, 985, 1705, 1865, 1900 |
| MfeI | CAATTG | 2 cut(s) | 1083, 1879 |
| MflI | RGATCY | 1 cut(s) | 1900 |
| MhlI | GDGCHC | 4 cut(s) | 748, 1574, 1612, 1828 |
| MlsI | TGGCCA | 4 cut(s) | 448, 755, 1733, 2216 |
| MluNI | TGGCCA | 4 cut(s) | 448, 755, 1733, 2216 |
| Mly113I | GGCGCC | 1 cut(s) | 737 |
| MlyI | GAGTC | 5 cut(s) | 1036, 1291, 1633, 1773, 2067 |
| MmeI | TCCRAC | 3 cut(s) | 120, 1641, 2035 |
| Mox20I | TGGCCA | 4 cut(s) | 448, 755, 1733, 2216 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1495 |
| MscI | TGGCCA | 4 cut(s) | 448, 755, 1733, 2216 |
| MslI | CAYNNNNRTG | 4 cut(s) | 113, 496, 1037, 1499 |
| Msp20I | TGGCCA | 4 cut(s) | 448, 755, 1733, 2216 |
| MspA1I | CMGCKG | 4 cut(s) | 674, 729, 1583, 2036 |
| MspCI | CTTAAG | 1 cut(s) | 238 |
| MunI | CAATTG | 2 cut(s) | 1083, 1879 |
| MvaI | CCWGG | 3 cut(s) | 297, 1154, 1670 |
| MvnI | CGCG | 2 cut(s) | 336, 1793 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 743, 752, 1205, 1589, 1739 |
| NarI | GGCGCC | 1 cut(s) | 737 |
| NciI | CCSGG | 7 cut(s) | 439, 691, 717, 1053, 1461, 1775, 1776 |
| NcoI | CCATGG | 2 cut(s) | 1563, 1734 |
| NdeI | CATATG | 1 cut(s) | 2193 |
| NdeII | GATC | 5 cut(s) | 148, 985, 1705, 1865, 1900 |
| NlaIV | GGNNCC | 4 cut(s) | 415, 738, 849, 1687 |
| NmuCI | GTSAC | 6 cut(s) | 49, 607, 989, 1103, 1987, 2029 |
| NspI | RCATGY | 2 cut(s) | 513, 1580 |
| PagI | TCATGA | 1 cut(s) | 88 |
| PceI | AGGCCT | 1 cut(s) | 281 |
| PciSI | GCTCTTC | 1 cut(s) | 541 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 347, 885, 1505 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1495 |
| PfeI | GAWTC | 7 cut(s) | 92, 119, 179, 585, 948, 1025, 1943 |
| PflFI | GACNNNGTC | 1 cut(s) | 1657 |
| PfoI | TCCNGGA | 1 cut(s) | 1668 |
| PkrI | GCNGC | 8 cut(s) | 592, 673, 731, 973, 1480, 1582, 1594, 1633 |
| PleI | GAGTC | 5 cut(s) | 1036, 1290, 1632, 1773, 2066 |
| PluTI | GGCGCC | 1 cut(s) | 740 |
| PmaCI | CACGTG | 1 cut(s) | 751 |
| PmlI | CACGTG | 1 cut(s) | 751 |
| PpsI | GAGTC | 5 cut(s) | 1036, 1290, 1632, 1773, 2066 |
| Ppu21I | YACGTR | 2 cut(s) | 751, 1810 |
| PpuMI | RGGWCCY | 3 cut(s) | 847, 920, 1525 |
| Psp5II | RGGWCCY | 3 cut(s) | 847, 920, 1525 |
| Psp6I | CCWGG | 3 cut(s) | 295, 1152, 1668 |
| PspCI | CACGTG | 1 cut(s) | 751 |
| PspEI | GGTNACC | 2 cut(s) | 1987, 2159 |
| PspFI | CCCAGC | 1 cut(s) | 1583 |
| PspGI | CCWGG | 3 cut(s) | 295, 1152, 1668 |
| PspN4I | GGNNCC | 4 cut(s) | 415, 738, 849, 1687 |
| PspPI | GGNCC | 5 cut(s) | 847, 920, 1412, 1525, 1765 |
| PspPPI | RGGWCCY | 3 cut(s) | 847, 920, 1525 |
| PstI | CTGCAG | 1 cut(s) | 1633 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1528 |
| PsuI | RGATCY | 1 cut(s) | 1900 |
| PsyI | GACNNNGTC | 1 cut(s) | 1657 |
| PvuII | CAGCTG | 2 cut(s) | 1583, 2036 |
| RseI | CAYNNNNRTG | 4 cut(s) | 113, 496, 1037, 1499 |
| SalI | GTCGAC | 1 cut(s) | 2011 |
| SapI | GCTCTTC | 1 cut(s) | 541 |
| SatI | GCNGC | 8 cut(s) | 591, 672, 730, 972, 1479, 1581, 1593, 1632 |
| Sau3AI | GATC | 5 cut(s) | 148, 985, 1705, 1865, 1900 |
| Sau96I | GGNCC | 5 cut(s) | 847, 920, 1412, 1525, 1765 |
| SchI | GAGTC | 5 cut(s) | 1036, 1291, 1633, 1773, 2067 |
| SduI | GDGCHC | 4 cut(s) | 748, 1574, 1612, 1828 |
| SfaNI | GCATC | 3 cut(s) | 704, 1107, 1186 |
| SfcI | CTRYAG | 2 cut(s) | 174, 1629 |
| SfoI | GGCGCC | 1 cut(s) | 738 |
| SinI | GGWCC | 4 cut(s) | 847, 920, 1412, 1525 |
| SmaI | CCCGGG | 1 cut(s) | 1776 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 113, 496, 1037, 1499 |
| SmlI | CTYRAG | 4 cut(s) | 238, 1169, 1439, 1968 |
| SmoI | CTYRAG | 4 cut(s) | 238, 1169, 1439, 1968 |
| SnaBI | TACGTA | 1 cut(s) | 1810 |
| SpeI | ACTAGT | 1 cut(s) | 1394 |
| SseBI | AGGCCT | 1 cut(s) | 281 |
| SsiI | CCGC | 4 cut(s) | 243, 672, 729, 1029 |
| SspDI | GGCGCC | 1 cut(s) | 736 |
| SspMI | CTAG | 5 cut(s) | 975, 1395, 1407, 1722, 2257 |
| StuI | AGGCCT | 1 cut(s) | 281 |
| StyI | CCWWGG | 5 cut(s) | 81, 953, 1563, 1734, 1839 |
| TaaI | ACNGT | 7 cut(s) | 175, 644, 794, 967, 1390, 1987, 2149 |
| TaiI | ACGT | 3 cut(s) | 753, 1661, 1812 |
| TaqI | TCGA | 4 cut(s) | 879, 1489, 1874, 2012 |
| TatI | WGTACW | 5 cut(s) | 30, 184, 759, 1957, 2236 |
| TauI | GCSGC | 2 cut(s) | 674, 732 |
| TfiI | GAWTC | 7 cut(s) | 92, 119, 179, 585, 948, 1025, 1943 |
| TscAI | CASTG | 3 cut(s) | 178, 256, 2185 |
| TseFI | GTSAC | 6 cut(s) | 49, 607, 989, 1103, 1987, 2029 |
| TseI | GCWGC | 6 cut(s) | 590, 971, 1478, 1580, 1592, 1631 |
| Tsp45I | GTSAC | 6 cut(s) | 49, 607, 989, 1103, 1987, 2029 |
| TspGWI | ACGGA | 1 cut(s) | 1881 |
| TspMI | CCCGGG | 1 cut(s) | 1774 |
| TspRI | CASTG | 3 cut(s) | 178, 256, 2185 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1657 |
| Vha464I | CTTAAG | 1 cut(s) | 238 |
| VneI | GTGCAC | 2 cut(s) | 1608, 1824 |
| VpaK11BI | GGWCC | 4 cut(s) | 847, 920, 1412, 1525 |
| XapI | RAATTY | 1 cut(s) | 1538 |
| XbaI | TCTAGA | 1 cut(s) | 1406 |
| XceI | RCATGY | 2 cut(s) | 513, 1580 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 424 |
| XmaI | CCCGGG | 1 cut(s) | 1774 |
| XmiI | GTMKAC | 1 cut(s) | 2012 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1495 |
| XspI | CTAG | 5 cut(s) | 975, 1395, 1407, 1722, 2257 |