FvH4_6g01060

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001268
Physical Location & Seq
Reverse (-)
578612 .. 580921
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01060.t1

Sequence Viewer

Length: 2310 bp
ATGGGGATCAATTGTGGTGTTCCTCTTCCTTACATTCTCTATGTTCTCCAGACTTTTCTGTTAGCTCTCCATTTCAACACTTCTTTGGCAGAGAGATCCACTTATATTGTCCACATGGACAAGTCTCTGATGCCCAAGTTTTTCACTAGCCATCAACATTGGTATTCCTCCATTGTTGATTCCTTCAAAACTGAAACTCAAACAGATAGCCACAGCTTCTCACCATCCATTTTATACACCTATGACAATGCATTCCATGGTTTTTCTGCAGTTCTTTCTATAGATGAACTCGAGACCCTAAAAAAGTCCCCCGGTTTTGTTTCAGCTTACAATGACAAAACTTTCACACTTGACACAACCCATACCAGTGACTTCCTCTCCCTCAACCCTTCTGCCGGCCTTTTGCCGGCCTCGAATCATGAAGAAGACATCGTCATTGGTGTCATTGATACCGGTGTCTGGCCAGAAAGCGAGAGCTTCAAAGACGATGGAGTCATGACCAAAGACATTCCGGCTAGGTGGAAGGGAACATGTGAGGTTGGACAAGAGTTCAATGCCTCTTTGTGTAACAAAAAGTTGATTGGAGCTAGATACTTTAACAAGGGAATTATGGCTGCAAACCCCGGCGTCACACTTAGCATGAACTCTGCTAGGGACAGTGAAGGCCATGGGACTCATACATCCTCCACCGCTGCAGGGAACTTTGTCAATGGCGCATCTTATTTTGGCTACGCTAATGGAACAGCCAGAGGTGTCGCACCACGCTCAAGGGTTGCAATGTACAAGGTCATTTGGGATGAGGGGCGTTACGCGTCTGATGTTCTAGCTGGTATAGACCAAGCTATTGCTGACGGTGTGGATGTAATTTCCTTGTCCATGGGGTTTGATGGAACGCCACTCTATGAAGATCCTATAGCAATAGCATCTTTTGCAGCCATGGAGAAGAATGTGGTGGTTTCAACTTCAGCCGGAAACGATGGCCCTGGCCTTGGATGGTTGCACAATGGTATTCCTTGGGTCTTGACTGTTGCAGCTGGCACTATAGACCGCTCCTTTGGCGGAATACTAACTCTTGGGAATGGTTTAACCATTACTGGGTCTAGCTTGTTACTAGAAAATGCCATAGTAGAAAAGGTTCCACTGGTGTACAACAAGACCTTCTCAGTTTGCAACTCGACAAAGGTACTATCAAATGCCCCAGAGGGAGTCATCATCTTATGTGACGAGAGTGATGATATTCGATTTCAGATATCTCAGTTCATTGGAATACATGTGCTTGGGGCTATTTTTATTGGCAAAGATCCTGAAATATTTGATGAAGGAGGTGTGACATTTCCTCTCGTGGTGGTAAGTCCGGATGATGCATCAGCACTAATCAAGTATGCTACAAGTAGCGATGAACCTTCTGTGACCATGAAATTCCAACAAACATTTCTTGGCACCAAGCCTGCACCGGCTGTGGCATCTTACACTTCAAGAGGACCTTCACCAGCTTATCCAGGAATCATGAAGCCGGACATAATGGCACCGGGGTCATTAGTTTTGGCTGCATGGCCTCCGAAGGTTGCAGCAGCCCGAGTTGGGTCGGATGTGATCTTTTCAAGTGACTACAATATGATATCTGGCACATCCATGGCTTGCCCTCATGCCTCAGGCGTAGCTGCTCTATTGAAAGGTGCACACCCCGAATGGAGTGCAGCTGCAATTAGGTCTGCCATGGTGACCACAGCCAACCCACTGGATAACACTCTGAATCCAATTCGTGACAATGGTGATCATAACTTCAACCCTGCTTCACCTTTGGCAATGGGAGCAGGTCAGGTGGATCCTAATCGAGCACTTGAACCAGGCTTAATATACGATGCAACCCCTCAAGACTATGTCAATCTCTTGTGCTCCACAAATTTTACCGCTAAGCAAATCTTAGCCATCACTAGATCACCTGCCTATGATTGTTTCAATCCATCTTCTGATCTGAACTATCCCTCCTTCATTGCTTTGTATGATCGTCGTCATGACACAAAGAAGCTAAAGGTCCAAAAATTTCAGAGGACAGTAACAAATGTGGGACATGGTGCAGCTACGTACAAGGCTTCGGTGATAGCTCCACAGGGTTCTAAAGTGACAGTGTCACCGGATACATTGTTCTTTTCAGAGAAATATGAGAAGCAAAGCTACACTCTTACAATAAGTTACAAGGGGAAAAAGAAAGGAAATGTGTCATTTGGTGAAATTGTTTGGGCGGTAGTAGATTTAAAGTACATGGTCAGGAGCCCCATTGTAGTGTCTCCTCTTGTTTCAGTGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

770

Amino Acids

82.58

Weight (kDa)

5.66

Isoelectric Point (pI)

31.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 117 4.7e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 143 - 588 2.5e-44 Subtilase family
fn3_6 PF17766 658 - 762 3.2e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1951
AasI GACNNNNNNGTC 1 cut(s) 491
Acc36I ACCTGC 2 cut(s) 1805, 1951
AccB1I GGYRCC 2 cut(s) 1439, 1525
AccBSI CCGCTC 1 cut(s) 1050
AccII CGCG 1 cut(s) 812
AccIII TCCGGA 1 cut(s) 1354
AciI CCGC 5 cut(s) 690, 1048, 1059, 1911, 2243
AclWI GGATC 6 cut(s) 14, 90, 902, 1295, 1820, 1833
AcoI YGGCCR 1 cut(s) 461
AcsI RAATTY 3 cut(s) 1418, 1903, 2042
AcuI CTGAAG 1 cut(s) 948
AcyI GRCGYC 1 cut(s) 627
AfaI GTAC 5 cut(s) 782, 1148, 1185, 2087, 2261
AfiI CCNNNNNNNGG 7 cut(s) 395, 406, 459, 696, 989, 1095, 1581
AflIII ACRYGT 3 cut(s) 530, 810, 1270
AgeI ACCGGT 1 cut(s) 452
AjnI CCWGG 3 cut(s) 982, 1498, 1846
AjuI GAANNNNNNNTTGG 2 cut(s) 1416, 1448
AloI GAACNNNNNNTCC 4 cut(s) 1970, 2002, 2129, 2161
Alw21I GWGCWC 3 cut(s) 1681, 1840, 1898
Alw26I GTCTC 3 cut(s) 129, 287, 2292
Alw44I GTGCAC 1 cut(s) 1677
AlwI GGATC 6 cut(s) 14, 90, 902, 1295, 1820, 1833
Ama87I CYCGRG 2 cut(s) 290, 1575
Aor13HI TCCGGA 1 cut(s) 1354
AoxI GGCC 7 cut(s) 397, 408, 461, 664, 979, 985, 1553
ApaLI GTGCAC 1 cut(s) 1677
ApoI RAATTY 3 cut(s) 1418, 1903, 2042
ArsI GACNNNNNNTTYG 2 cut(s) 1037, 1069
AsiGI ACCGGT 1 cut(s) 452
Asp700I GAANNNNTTC 1 cut(s) 1134
AspLEI GCGC 1 cut(s) 716
AspS9I GGNCC 3 cut(s) 980, 1481, 2035
AsuC2I CCSGG 3 cut(s) 312, 624, 1530
AsuHPI GGTGA 9 cut(s) 213, 1479, 1732, 1784, 1788, 1932, 2110, 2124, 2240
AvaI CYCGRG 2 cut(s) 290, 1575
AvaII GGWCC 2 cut(s) 1481, 2035
AxyI CCTNAGG 1 cut(s) 1651
BaeGI GKGCMC 1 cut(s) 1681
BaeI ACNNNNGTAYC 2 cut(s) 441, 474
BalI TGGCCA 1 cut(s) 463
BamHI GGATCC 1 cut(s) 1825
BanI GGYRCC 2 cut(s) 1439, 1525
BanII GRGCYC 1 cut(s) 2276
BarI GAAGNNNNNNTAC 2 cut(s) 2159, 2191
BauI CACGAG 1 cut(s) 1340
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 3 cut(s) 1681, 1840, 1898
BccI CCATC 8 cut(s) 159, 232, 482, 881, 971, 987, 1937, 1972
BcgI CGANNNNNNTGC 2 cut(s) 1676, 1710
BciT130I CCWGG 3 cut(s) 984, 1500, 1848
BciVI GTATCC 1 cut(s) 2131
BclI TGATCA 1 cut(s) 1774
BcnI CCSGG 3 cut(s) 312, 624, 1530
BcoDI GTCTC 3 cut(s) 129, 287, 2292
BfaI CTAG 8 cut(s) 147, 516, 588, 651, 824, 1101, 1112, 1935
BfmI CTRYAG 5 cut(s) 267, 279, 693, 912, 1041
BfuAI ACCTGC 2 cut(s) 1805, 1951
BfuI GTATCC 1 cut(s) 2131
BlpI GCTNAGC 1 cut(s) 1914
Bme1390I CCNGG 6 cut(s) 312, 624, 984, 1500, 1530, 1848
Bme18I GGWCC 2 cut(s) 1481, 2035
BmeT110I CYCGRG 2 cut(s) 290, 1575
BmgT120I GGNCC 3 cut(s) 980, 1481, 2035
BmiI GGNNCC 5 cut(s) 1137, 1441, 1527, 1827, 2273
BmrFI CCNGG 6 cut(s) 312, 624, 984, 1500, 1530, 1848
BmrI ACTGGG 1 cut(s) 1104
BmsI GCATC 7 cut(s) 120, 725, 932, 1351, 1373, 1472, 1852
BmuI ACTGGG 1 cut(s) 1104
BpiI GAAGAC 1 cut(s) 432
BpmI CTGGAG 1 cut(s) 32
Bpu1102I GCTNAGC 1 cut(s) 1914
BpuEI CTTGAG 2 cut(s) 751, 1857
BpuMI CCSGG 3 cut(s) 312, 624, 1530
BsaAI YACGTR 1 cut(s) 2085
BsaBI GATNNNNATC 1 cut(s) 1830
BsaHI GRCGYC 1 cut(s) 627
BsaI GGTCTC 1 cut(s) 287
BsaWI WCCGGW 3 cut(s) 452, 1354, 2134
BsaXI ACNNNNNCTCC 4 cut(s) 362, 392, 1970, 2000
Bsc4I CCNNNNNNNGG 7 cut(s) 395, 406, 459, 696, 989, 1095, 1581
Bse118I RCCGGY 4 cut(s) 395, 406, 452, 1453
Bse1I ACTGG 4 cut(s) 366, 1099, 1146, 1743
Bse21I CCTNAGG 1 cut(s) 1651
Bse3DI GCAATG 3 cut(s) 783, 1812, 1992
Bse8I GATNNNNATC 1 cut(s) 1830
BseAI TCCGGA 1 cut(s) 1354
BseBI CCWGG 3 cut(s) 984, 1500, 1848
BseGI GGATG 8 cut(s) 224, 680, 802, 865, 998, 1363, 1594, 1628
BseJI GATNNNNATC 1 cut(s) 1830
BseLI CCNNNNNNNGG 7 cut(s) 395, 406, 459, 696, 989, 1095, 1581
BseMI GCAATG 3 cut(s) 783, 1812, 1992
BseMII CTCAG 3 cut(s) 1176, 1268, 1665
BseNI ACTGG 4 cut(s) 366, 1099, 1146, 1743
BseRI GAGGAG 1 cut(s) 2280
BseSI GKGCMC 1 cut(s) 1681
BsgI GTGCAG 3 cut(s) 1434, 1716, 2097
Bsh1236I CGCG 1 cut(s) 812
BshFI GGCC 7 cut(s) 399, 410, 463, 666, 981, 987, 1555
BshNI GGYRCC 2 cut(s) 1439, 1525
BshTI ACCGGT 1 cut(s) 452
BsiHKAI GWGCWC 3 cut(s) 1681, 1840, 1898
BsiHKCI CYCGRG 2 cut(s) 290, 1575
BslFI GGGAC 4 cut(s) 292, 668, 685, 2082
BslI CCNNNNNNNGG 7 cut(s) 395, 406, 459, 696, 989, 1095, 1581
BsmAI GTCTC 3 cut(s) 129, 287, 2292
BsmFI GGGAC 4 cut(s) 292, 668, 685, 2082
BsmI GAATGC 1 cut(s) 251
BsnI GGCC 7 cut(s) 399, 410, 463, 666, 981, 987, 1555
Bso31I GGTCTC 1 cut(s) 287
BsoBI CYCGRG 2 cut(s) 290, 1575
Bsp1286I GDGCHC 4 cut(s) 1681, 1840, 1898, 2276
Bsp13I TCCGGA 1 cut(s) 1354
Bsp1407I TGTACA 2 cut(s) 780, 1146
Bsp1720I GCTNAGC 1 cut(s) 1914
Bsp19I CCATGG 6 cut(s) 256, 667, 876, 936, 1632, 1716
BspACI CCGC 5 cut(s) 690, 1048, 1059, 1911, 2243
BspANI GGCC 7 cut(s) 399, 410, 463, 666, 981, 987, 1555
BspCNI CTCAG 3 cut(s) 1175, 1267, 1664
BspEI TCCGGA 1 cut(s) 1354
BspFNI CGCG 1 cut(s) 812
BspHI TCATGA 4 cut(s) 418, 495, 1506, 2014
BspLI GGNNCC 5 cut(s) 1137, 1441, 1527, 1827, 2273
BspMAI CTGCAG 2 cut(s) 271, 697
BspMI ACCTGC 2 cut(s) 1805, 1951
BspPI GGATC 6 cut(s) 14, 90, 902, 1295, 1820, 1833
BspT107I GGYRCC 2 cut(s) 1439, 1525
BspTNI GGTCTC 1 cut(s) 287
BsrBI CCGCTC 1 cut(s) 1050
BsrDI GCAATG 3 cut(s) 783, 1812, 1992
BsrFI RCCGGY 4 cut(s) 395, 406, 452, 1453
BsrGI TGTACA 2 cut(s) 780, 1146
BsrI ACTGG 4 cut(s) 366, 1099, 1146, 1743
BssAI RCCGGY 4 cut(s) 395, 406, 452, 1453
BssNI GRCGYC 1 cut(s) 627
BssSI CACGAG 1 cut(s) 1340
BssT1I CCWWGG 8 cut(s) 256, 667, 876, 936, 988, 1013, 1632, 1716
Bst2BI CACGAG 1 cut(s) 1340
Bst2UI CCWGG 3 cut(s) 984, 1500, 1848
Bst4CI ACNGT 5 cut(s) 659, 854, 1027, 2056, 2128
Bst6I CTCTTC 1 cut(s) 30
BstACI GRCGYC 1 cut(s) 627
BstAPI GCANNNNNTGC 1 cut(s) 929
BstAUI TGTACA 2 cut(s) 780, 1146
BstBAI YACGTR 1 cut(s) 2085
BstC8I GCNNGC 5 cut(s) 397, 408, 1036, 1449, 1639
BstDEI CTNAG 6 cut(s) 635, 1162, 1254, 1651, 1914, 1924
BstDSI CCRYGG 6 cut(s) 256, 667, 876, 936, 1632, 1716
BstEII GGTNACC 1 cut(s) 1720
BstF5I GGATG 8 cut(s) 224, 680, 802, 865, 998, 1363, 1594, 1628
BstFNI CGCG 1 cut(s) 812
BstHHI GCGC 1 cut(s) 716
BstMAI GTCTC 3 cut(s) 129, 287, 2292
BstMWI GCNNNNNNNGC 3 cut(s) 929, 1056, 1811
BstNI CCWGG 3 cut(s) 984, 1500, 1848
BstNSI RCATGY 2 cut(s) 534, 1274
BstPI GGTNACC 1 cut(s) 1720
BstSCI CCNGG 6 cut(s) 310, 622, 982, 1498, 1528, 1846
BstSFI CTRYAG 5 cut(s) 267, 279, 693, 912, 1041
BstSLI GKGCMC 1 cut(s) 1681
BstSNI TACGTA 1 cut(s) 2085
BstUI CGCG 1 cut(s) 812
BstV2I GAAGAC 1 cut(s) 432
BstX2I RGATCY 4 cut(s) 95, 907, 1300, 1825
BstXI CCANNNNNNTGG 1 cut(s) 1738
BstYI RGATCY 4 cut(s) 95, 907, 1300, 1825
Bsu36I CCTNAGG 1 cut(s) 1651
BsuI GTATCC 1 cut(s) 2131
BsuRI GGCC 7 cut(s) 399, 410, 463, 666, 981, 987, 1555
BtgI CCRYGG 6 cut(s) 256, 667, 876, 936, 1632, 1716
BtgZI GCGATG 1 cut(s) 1410
BtsCI GGATG 8 cut(s) 224, 680, 802, 865, 998, 1363, 1594, 1628
BtsIMutI CAGTG 6 cut(s) 373, 664, 1139, 1736, 2133, 2307
BveI ACCTGC 2 cut(s) 1805, 1951
Cac8I GCNNGC 5 cut(s) 397, 408, 1036, 1449, 1639
CciI TCATGA 4 cut(s) 418, 495, 1506, 2014
CfoI GCGC 1 cut(s) 716
Cfr10I RCCGGY 4 cut(s) 395, 406, 452, 1453
Cfr13I GGNCC 3 cut(s) 980, 1481, 2035
CseI GACGC 2 cut(s) 616, 801
Csp6I GTAC 5 cut(s) 781, 1147, 1184, 2086, 2260
CspAI ACCGGT 1 cut(s) 452
CspCI CAANNNNNGTGG 2 cut(s) 88, 123
CviQI GTAC 5 cut(s) 781, 1147, 1184, 2086, 2260
DdeI CTNAG 6 cut(s) 635, 1162, 1254, 1651, 1914, 1924
DraI TTTAAA 1 cut(s) 2256
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
EaeI YGGCCR 1 cut(s) 461
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
EciI GGCGGA 1 cut(s) 1074
Eco105I TACGTA 1 cut(s) 2085
Eco130I CCWWGG 8 cut(s) 256, 667, 876, 936, 988, 1013, 1632, 1716
Eco24I GRGCYC 1 cut(s) 2276
Eco31I GGTCTC 1 cut(s) 287
Eco32I GATATC 2 cut(s) 1251, 1620
Eco47I GGWCC 2 cut(s) 1481, 2035
Eco57I CTGAAG 1 cut(s) 948
Eco81I CCTNAGG 1 cut(s) 1651
Eco88I CYCGRG 2 cut(s) 290, 1575
Eco91I GGTNACC 1 cut(s) 1720
EcoO109I RGGNCCY 1 cut(s) 1481
EcoO65I GGTNACC 1 cut(s) 1720
EcoRII CCWGG 3 cut(s) 982, 1498, 1846
EcoRV GATATC 2 cut(s) 1251, 1620
EcoT14I CCWWGG 8 cut(s) 256, 667, 876, 936, 988, 1013, 1632, 1716
EcoT22I ATGCAT 2 cut(s) 253, 1366
EcoT38I GRGCYC 1 cut(s) 2276
ErhI CCWWGG 8 cut(s) 256, 667, 876, 936, 988, 1013, 1632, 1716
FalI AAGNNNNNCTT 4 cut(s) 1468, 1500, 1907, 1939
FaqI GGGAC 4 cut(s) 292, 668, 685, 2082
FbaI TGATCA 1 cut(s) 1774
FokI GGATG 8 cut(s) 211, 667, 809, 872, 1005, 1370, 1601, 1615
FriOI GRGCYC 1 cut(s) 2276
FspBI CTAG 8 cut(s) 147, 516, 588, 651, 824, 1101, 1112, 1935
GlaI GCGC 1 cut(s) 715
GsuI CTGGAG 1 cut(s) 32
HaeIII GGCC 7 cut(s) 399, 410, 463, 666, 981, 987, 1555
HgaI GACGC 2 cut(s) 616, 801
HhaI GCGC 1 cut(s) 716
Hin1I GRCGYC 1 cut(s) 627
Hin6I GCGC 1 cut(s) 714
HinP1I GCGC 1 cut(s) 714
HinfI GANTC 7 cut(s) 179, 415, 492, 673, 1206, 1503, 1753
HphI GGTGA 9 cut(s) 213, 1479, 1732, 1784, 1788, 1932, 2110, 2124, 2240
Hpy166II GTNNAC 3 cut(s) 112, 1147, 1679
Hpy8I GTNNAC 3 cut(s) 112, 1147, 1679
Hpy99I CGWCG 1 cut(s) 2013
HpyCH4III ACNGT 5 cut(s) 659, 854, 1027, 2056, 2128
HpyCH4IV ACGT 1 cut(s) 2084
HpyF10VI GCNNNNNNNGC 3 cut(s) 929, 1056, 1811
HpyF3I CTNAG 6 cut(s) 635, 1162, 1254, 1651, 1914, 1924
HpySE526I ACGT 1 cut(s) 2084
Hsp92I GRCGYC 1 cut(s) 627
HspAI GCGC 1 cut(s) 714
Kpn2I TCCGGA 1 cut(s) 1354
KroI GCCGGC 2 cut(s) 395, 406
KroNI GCCGGC 2 cut(s) 397, 408
Ksp22I TGATCA 1 cut(s) 1774
LmnI GCTCC 6 cut(s) 584, 1055, 1811, 1901, 2110, 2271
LweI GCATC 7 cut(s) 120, 725, 932, 1351, 1373, 1472, 1852
MaeI CTAG 8 cut(s) 147, 516, 588, 651, 824, 1101, 1112, 1935
MaeII ACGT 1 cut(s) 2084
MbiI CCGCTC 1 cut(s) 1050
MboII GAAGA 6 cut(s) 17, 434, 437, 917, 955, 1959
MfeI CAATTG 1 cut(s) 10
MflI RGATCY 4 cut(s) 95, 907, 1300, 1825
MhlI GDGCHC 4 cut(s) 1681, 1840, 1898, 2276
MlsI TGGCCA 1 cut(s) 463
MluCI AATT 9 cut(s) 10, 606, 864, 1418, 1704, 1758, 1903, 2042, 2232
MluI ACGCGT 1 cut(s) 810
MluNI TGGCCA 1 cut(s) 463
MlyI GAGTC 3 cut(s) 501, 667, 1215
MmeI TCCRAC 3 cut(s) 520, 1447, 1566
Mox20I TGGCCA 1 cut(s) 463
Mph1103I ATGCAT 2 cut(s) 253, 1366
MroI TCCGGA 1 cut(s) 1354
MroNI GCCGGC 2 cut(s) 395, 406
MroXI GAANNNNTTC 1 cut(s) 1134
MscI TGGCCA 1 cut(s) 463
MseI TTAA 5 cut(s) 597, 1085, 1853, 2255, 2308
MslI CAYNNNNRTG 3 cut(s) 366, 1631, 2282
Msp20I TGGCCA 1 cut(s) 463
MspA1I CMGCKG 3 cut(s) 692, 1034, 1700
MspR9I CCNGG 6 cut(s) 312, 624, 984, 1500, 1530, 1848
MunI CAATTG 1 cut(s) 10
Mva1269I GAATGC 1 cut(s) 251
MvaI CCWGG 3 cut(s) 984, 1500, 1848
MvnI CGCG 1 cut(s) 812
MwoI GCNNNNNNNGC 3 cut(s) 929, 1056, 1811
NaeI GCCGGC 2 cut(s) 397, 408
NciI CCSGG 3 cut(s) 312, 624, 1530
NcoI CCATGG 6 cut(s) 256, 667, 876, 936, 1632, 1716
NgoMIV GCCGGC 2 cut(s) 395, 406
NlaIV GGNNCC 5 cut(s) 1137, 1441, 1527, 1827, 2273
NsiI ATGCAT 2 cut(s) 253, 1366
NspI RCATGY 2 cut(s) 534, 1274
PaeR7I CTCGAG 1 cut(s) 290
PagI TCATGA 4 cut(s) 418, 495, 1506, 2014
PaqCI CACCTGC 1 cut(s) 1951
PciI ACATGT 2 cut(s) 530, 1270
PctI GAATGC 1 cut(s) 251
PdiI GCCGGC 2 cut(s) 397, 408
PdmI GAANNNNTTC 1 cut(s) 1134
PfeI GAWTC 4 cut(s) 179, 415, 1503, 1753
PflFI GACNNNGTC 3 cut(s) 431, 1880, 2128
PfoI TCCNGGA 1 cut(s) 1498
PinAI ACCGGT 1 cut(s) 452
PleI GAGTC 3 cut(s) 500, 667, 1214
PpsI GAGTC 3 cut(s) 500, 667, 1214
Ppu21I YACGTR 1 cut(s) 2085
PpuMI RGGWCCY 1 cut(s) 1481
PscI ACATGT 2 cut(s) 530, 1270
Psp5II RGGWCCY 1 cut(s) 1481
Psp6I CCWGG 3 cut(s) 982, 1498, 1846
PspEI GGTNACC 1 cut(s) 1720
PspGI CCWGG 3 cut(s) 982, 1498, 1846
PspN4I GGNNCC 5 cut(s) 1137, 1441, 1527, 1827, 2273
PspPI GGNCC 3 cut(s) 980, 1481, 2035
PspPPI RGGWCCY 1 cut(s) 1481
PsrI GAACNNNNNNTAC 2 cut(s) 1119, 1151
PstI CTGCAG 2 cut(s) 271, 697
PsuI RGATCY 4 cut(s) 95, 907, 1300, 1825
PsyI GACNNNGTC 3 cut(s) 431, 1880, 2128
PvuII CAGCTG 2 cut(s) 1034, 1700
RsaI GTAC 5 cut(s) 782, 1148, 1185, 2087, 2261
RsaNI GTAC 5 cut(s) 781, 1147, 1184, 2086, 2260
RseI CAYNNNNRTG 3 cut(s) 366, 1631, 2282
SaqAI TTAA 5 cut(s) 597, 1085, 1853, 2255, 2308
Sau96I GGNCC 3 cut(s) 980, 1481, 2035
SchI GAGTC 3 cut(s) 501, 667, 1215
ScrFI CCNGG 6 cut(s) 312, 624, 984, 1500, 1530, 1848
SduI GDGCHC 4 cut(s) 1681, 1840, 1898, 2276
SfaNI GCATC 7 cut(s) 120, 725, 932, 1351, 1373, 1472, 1852
SfcI CTRYAG 5 cut(s) 267, 279, 693, 912, 1041
Sfr274I CTCGAG 1 cut(s) 290
SinI GGWCC 2 cut(s) 1481, 2035
SlaI CTCGAG 1 cut(s) 290
SmiMI CAYNNNNRTG 3 cut(s) 366, 1631, 2282
SmlI CTYRAG 3 cut(s) 290, 766, 1872
SmoI CTYRAG 3 cut(s) 290, 766, 1872
SnaBI TACGTA 1 cut(s) 2085
Sse9I AATT 9 cut(s) 10, 606, 864, 1418, 1704, 1758, 1903, 2042, 2232
SsiI CCGC 5 cut(s) 690, 1048, 1059, 1911, 2243
SspI AATATT 1 cut(s) 1311
SspMI CTAG 8 cut(s) 147, 516, 588, 651, 824, 1101, 1112, 1935
StyD4I CCNGG 6 cut(s) 310, 622, 982, 1498, 1528, 1846
StyI CCWWGG 8 cut(s) 256, 667, 876, 936, 988, 1013, 1632, 1716
TaaI ACNGT 5 cut(s) 659, 854, 1027, 2056, 2128
TaiI ACGT 1 cut(s) 2087
TaqI TCGA 5 cut(s) 291, 413, 1175, 1240, 1834
TasI AATT 9 cut(s) 10, 606, 864, 1418, 1704, 1758, 1903, 2042, 2232
TatI WGTACW 3 cut(s) 780, 1146, 2259
TfiI GAWTC 4 cut(s) 179, 415, 1503, 1753
Tru1I TTAA 5 cut(s) 597, 1085, 1853, 2255, 2308
Tru9I TTAA 5 cut(s) 597, 1085, 1853, 2255, 2308
TscAI CASTG 6 cut(s) 373, 664, 1146, 1743, 2133, 2307
TspRI CASTG 6 cut(s) 373, 664, 1146, 1743, 2133, 2307
Tth111I GACNNNGTC 3 cut(s) 431, 1880, 2128
VneI GTGCAC 1 cut(s) 1677
VpaK11BI GGWCC 2 cut(s) 1481, 2035
XapI RAATTY 3 cut(s) 1418, 1903, 2042
XceI RCATGY 2 cut(s) 534, 1274
XhoI CTCGAG 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 1134
XspI CTAG 8 cut(s) 147, 516, 588, 651, 824, 1101, 1112, 1935
Zsp2I ATGCAT 2 cut(s) 253, 1366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.