subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1848 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1540 |
| Acc36I | ACCTGC | 1 cut(s) | 1366 |
| Acc65I | GGTACC | 1 cut(s) | 914 |
| AccB1I | GGYRCC | 3 cut(s) | 461, 676, 914 |
| AccBSI | CCGCTC | 1 cut(s) | 288 |
| AccI | GTMKAC | 2 cut(s) | 753, 1287 |
| AccII | CGCG | 1 cut(s) | 573 |
| AccIII | TCCGGA | 1 cut(s) | 728 |
| AciI | CCGC | 4 cut(s) | 286, 571, 1250, 1607 |
| AclWI | GGATC | 3 cut(s) | 739, 1450, 1739 |
| AcoI | YGGCCR | 2 cut(s) | 1557, 2187 |
| AcsI | RAATTY | 4 cut(s) | 870, 984, 1328, 1921 |
| AcuI | CTGAAG | 5 cut(s) | 30, 172, 1121, 1439, 1506 |
| AfaI | GTAC | 6 cut(s) | 142, 661, 701, 916, 1351, 2081 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 320, 438, 911 |
| AhlI | ACTAGT | 2 cut(s) | 1472, 1753 |
| AjnI | CCWGG | 3 cut(s) | 904, 1438, 1747 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2105, 2137 |
| Alw21I | GWGCWC | 2 cut(s) | 688, 934 |
| Alw44I | GTGCAC | 1 cut(s) | 930 |
| AlwI | GGATC | 3 cut(s) | 739, 1450, 1739 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1193, 1484 |
| Ama87I | CYCGRG | 1 cut(s) | 389 |
| Aor13HI | TCCGGA | 1 cut(s) | 728 |
| AoxI | GGCC | 7 cut(s) | 329, 386, 504, 901, 1391, 1557, 2187 |
| ApaI | GGGCCC | 1 cut(s) | 508 |
| ApaLI | GTGCAC | 1 cut(s) | 930 |
| ApoI | RAATTY | 4 cut(s) | 870, 984, 1328, 1921 |
| AseI | ATTAAT | 2 cut(s) | 1448, 1878 |
| Asp718I | GGTACC | 1 cut(s) | 914 |
| AspLEI | GCGC | 4 cut(s) | 326, 635, 1549, 1573 |
| AspS9I | GGNCC | 8 cut(s) | 387, 504, 505, 902, 1391, 1464, 1839, 2099 |
| AsuC2I | CCSGG | 1 cut(s) | 508 |
| AsuHPI | GGTGA | 4 cut(s) | 65, 271, 359, 1274 |
| AsuII | TTCGAA | 1 cut(s) | 338 |
| AvaI | CYCGRG | 1 cut(s) | 389 |
| AvaII | GGWCC | 3 cut(s) | 1464, 1839, 2099 |
| BaeGI | GKGCMC | 2 cut(s) | 508, 934 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 651, 684, 906, 939 |
| BalI | TGGCCA | 1 cut(s) | 2189 |
| BanI | GGYRCC | 3 cut(s) | 461, 676, 914 |
| BanII | GRGCYC | 4 cut(s) | 508, 688, 1604, 2135 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2063, 2095 |
| Bbv12I | GWGCWC | 2 cut(s) | 688, 934 |
| BbvCI | CCTCAGC | 1 cut(s) | 126 |
| BccI | CCATC | 7 cut(s) | 432, 764, 893, 1030, 1087, 1265, 2147 |
| BceAI | ACGGC | 2 cut(s) | 734, 1650 |
| BciT130I | CCWGG | 3 cut(s) | 906, 1440, 1749 |
| BciVI | GTATCC | 2 cut(s) | 736, 1041 |
| BclI | TGATCA | 1 cut(s) | 70 |
| BcnI | CCSGG | 1 cut(s) | 508 |
| BcuI | ACTAGT | 2 cut(s) | 1472, 1753 |
| BfaI | CTAG | 4 cut(s) | 435, 1047, 1473, 1754 |
| BfmI | CTRYAG | 2 cut(s) | 666, 885 |
| BfoI | RGCGCY | 2 cut(s) | 327, 1550 |
| BfuAI | ACCTGC | 1 cut(s) | 1366 |
| BfuI | GTATCC | 2 cut(s) | 736, 1041 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1565 |
| BmcAI | AGTACT | 1 cut(s) | 142 |
| Bme1390I | CCNGG | 4 cut(s) | 508, 906, 1440, 1749 |
| Bme18I | GGWCC | 3 cut(s) | 1464, 1839, 2099 |
| BmeT110I | CYCGRG | 1 cut(s) | 389 |
| BmgT120I | GGNCC | 8 cut(s) | 387, 504, 505, 902, 1391, 1464, 1839, 2099 |
| BmrFI | CCNGG | 4 cut(s) | 508, 906, 1440, 1749 |
| BmsI | GCATC | 6 cut(s) | 644, 1466, 1597, 1663, 1753, 1918 |
| BpmI | CTGGAG | 1 cut(s) | 399 |
| Bpu10I | CCTNAGC | 2 cut(s) | 126, 2075 |
| Bpu14I | TTCGAA | 1 cut(s) | 338 |
| BpuMI | CCSGG | 1 cut(s) | 508 |
| Bsa29I | ATCGAT | 1 cut(s) | 966 |
| BsaWI | WCCGGW | 1 cut(s) | 728 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 320, 438, 911 |
| Bse118I | RCCGGY | 1 cut(s) | 1559 |
| Bse1I | ACTGG | 5 cut(s) | 138, 382, 661, 1284, 1986 |
| Bse3DI | GCAATG | 4 cut(s) | 702, 1689, 1806, 1893 |
| BseAI | TCCGGA | 1 cut(s) | 728 |
| BseBI | CCWGG | 3 cut(s) | 906, 1440, 1749 |
| BseCI | ATCGAT | 1 cut(s) | 966 |
| BseGI | GGATG | 5 cut(s) | 635, 817, 1022, 1588, 1933 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 320, 438, 911 |
| BseMI | GCAATG | 4 cut(s) | 702, 1689, 1806, 1893 |
| BseMII | CTCAG | 3 cut(s) | 117, 201, 1070 |
| BseNI | ACTGG | 5 cut(s) | 138, 382, 661, 1284, 1986 |
| BseSI | GKGCMC | 2 cut(s) | 508, 934 |
| BsgI | GTGCAG | 2 cut(s) | 975, 1344 |
| Bsh1236I | CGCG | 1 cut(s) | 573 |
| Bsh1285I | CGRYCG | 1 cut(s) | 970 |
| BshFI | GGCC | 7 cut(s) | 331, 388, 506, 903, 1393, 1559, 2189 |
| BshNI | GGYRCC | 3 cut(s) | 461, 676, 914 |
| BshVI | ATCGAT | 1 cut(s) | 966 |
| BsiEI | CGRYCG | 1 cut(s) | 970 |
| BsiHKAI | GWGCWC | 2 cut(s) | 688, 934 |
| BsiHKCI | CYCGRG | 1 cut(s) | 389 |
| BsiSI | CCGG | 5 cut(s) | 459, 508, 729, 891, 1560 |
| BslFI | GGGAC | 2 cut(s) | 1450, 1546 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 320, 438, 911 |
| BsmFI | GGGAC | 2 cut(s) | 1450, 1546 |
| BsmI | GAATGC | 1 cut(s) | 1171 |
| BsnI | GGCC | 7 cut(s) | 331, 388, 506, 903, 1393, 1559, 2189 |
| BsoBI | CYCGRG | 1 cut(s) | 389 |
| Bsp119I | TTCGAA | 1 cut(s) | 338 |
| Bsp120I | GGGCCC | 1 cut(s) | 504 |
| Bsp1286I | GDGCHC | 5 cut(s) | 508, 688, 934, 1604, 2135 |
| Bsp13I | TCCGGA | 1 cut(s) | 728 |
| Bsp1407I | TGTACA | 1 cut(s) | 699 |
| Bsp143I | GATC | 8 cut(s) | 70, 259, 731, 967, 1442, 1489, 1616, 1744 |
| Bsp19I | CCATGG | 1 cut(s) | 935 |
| BspACI | CCGC | 4 cut(s) | 286, 571, 1250, 1607 |
| BspANI | GGCC | 7 cut(s) | 331, 388, 506, 903, 1393, 1559, 2189 |
| BspCNI | CTCAG | 3 cut(s) | 118, 202, 1069 |
| BspDI | ATCGAT | 1 cut(s) | 966 |
| BspEI | TCCGGA | 1 cut(s) | 728 |
| BspFNI | CGCG | 1 cut(s) | 573 |
| BspHI | TCATGA | 1 cut(s) | 826 |
| BspMAI | CTGCAG | 1 cut(s) | 889 |
| BspMI | ACCTGC | 1 cut(s) | 1366 |
| BspPI | GGATC | 3 cut(s) | 739, 1450, 1739 |
| BspQI | GCTCTTC | 2 cut(s) | 295, 481 |
| BspT104I | TTCGAA | 1 cut(s) | 338 |
| BspT107I | GGYRCC | 3 cut(s) | 461, 676, 914 |
| BsrBI | CCGCTC | 1 cut(s) | 288 |
| BsrDI | GCAATG | 4 cut(s) | 702, 1689, 1806, 1893 |
| BsrFI | RCCGGY | 1 cut(s) | 1559 |
| BsrGI | TGTACA | 1 cut(s) | 699 |
| BsrI | ACTGG | 5 cut(s) | 138, 382, 661, 1284, 1986 |
| BssAI | RCCGGY | 1 cut(s) | 1559 |
| BssMI | GATC | 8 cut(s) | 70, 259, 731, 967, 1442, 1489, 1616, 1744 |
| BssT1I | CCWWGG | 8 cut(s) | 235, 301, 795, 935, 993, 1034, 1364, 2010 |
| Bst2UI | CCWGG | 3 cut(s) | 906, 1440, 1749 |
| Bst4CI | ACNGT | 6 cut(s) | 116, 361, 1342, 1382, 1910, 1983 |
| Bst6I | CTCTTC | 2 cut(s) | 295, 481 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1175 |
| BstAUI | TGTACA | 1 cut(s) | 699 |
| BstBI | TTCGAA | 1 cut(s) | 338 |
| BstC8I | GCNNGC | 5 cut(s) | 333, 571, 616, 974, 1696 |
| BstDEI | CTNAG | 6 cut(s) | 126, 210, 919, 1056, 2075, 2138 |
| BstDSI | CCRYGG | 2 cut(s) | 935, 1632 |
| BstEII | GGTNACC | 1 cut(s) | 1262 |
| BstF5I | GGATG | 5 cut(s) | 635, 817, 1022, 1588, 1933 |
| BstFNI | CGCG | 1 cut(s) | 573 |
| BstH2I | RGCGCY | 2 cut(s) | 327, 1550 |
| BstHHI | GCGC | 4 cut(s) | 326, 635, 1549, 1573 |
| BstKTI | GATC | 8 cut(s) | 73, 262, 734, 970, 1445, 1492, 1619, 1747 |
| BstMBI | GATC | 8 cut(s) | 70, 259, 731, 967, 1442, 1489, 1616, 1744 |
| BstMCI | CGRYCG | 1 cut(s) | 970 |
| BstNI | CCWGG | 3 cut(s) | 906, 1440, 1749 |
| BstPI | GGTNACC | 1 cut(s) | 1262 |
| BstSCI | CCNGG | 4 cut(s) | 506, 904, 1438, 1747 |
| BstSFI | CTRYAG | 2 cut(s) | 666, 885 |
| BstSLI | GKGCMC | 2 cut(s) | 508, 934 |
| BstUI | CGCG | 1 cut(s) | 573 |
| BstX2I | RGATCY | 1 cut(s) | 731 |
| BstYI | RGATCY | 1 cut(s) | 731 |
| Bsu15I | ATCGAT | 1 cut(s) | 966 |
| BsuI | GTATCC | 2 cut(s) | 736, 1041 |
| BsuRI | GGCC | 7 cut(s) | 331, 388, 506, 903, 1393, 1559, 2189 |
| BsuTUI | ATCGAT | 1 cut(s) | 966 |
| BtgI | CCRYGG | 2 cut(s) | 935, 1632 |
| BtsCI | GGATG | 5 cut(s) | 635, 817, 1022, 1588, 1933 |
| BtsI | GCAGTG | 1 cut(s) | 948 |
| BtsIMutI | CAGTG | 3 cut(s) | 196, 375, 948 |
| BveI | ACCTGC | 1 cut(s) | 1366 |
| Cac8I | GCNNGC | 5 cut(s) | 333, 571, 616, 974, 1696 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1193, 1484 |
| CciI | TCATGA | 1 cut(s) | 826 |
| CfoI | GCGC | 4 cut(s) | 326, 635, 1549, 1573 |
| Cfr10I | RCCGGY | 1 cut(s) | 1559 |
| Cfr13I | GGNCC | 8 cut(s) | 387, 504, 505, 902, 1391, 1464, 1839, 2099 |
| ClaI | ATCGAT | 1 cut(s) | 966 |
| Csp6I | GTAC | 6 cut(s) | 141, 660, 700, 915, 1350, 2080 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2028, 2063 |
| CviAII | CATG | 8 cut(s) | 37, 52, 587, 827, 859, 936, 1461, 1553 |
| CviQI | GTAC | 6 cut(s) | 141, 660, 700, 915, 1350, 2080 |
| DdeI | CTNAG | 6 cut(s) | 126, 210, 919, 1056, 2075, 2138 |
| DpnI | GATC | 8 cut(s) | 72, 261, 733, 969, 1444, 1491, 1618, 1746 |
| DpnII | GATC | 8 cut(s) | 70, 259, 731, 967, 1442, 1489, 1616, 1744 |
| DraI | TTTAAA | 1 cut(s) | 2022 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1540 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1540 |
| EaeI | YGGCCR | 2 cut(s) | 1557, 2187 |
| Eam1104I | CTCTTC | 2 cut(s) | 295, 481 |
| EarI | CTCTTC | 2 cut(s) | 295, 481 |
| Ecl136II | GAGCTC | 1 cut(s) | 686 |
| Eco130I | CCWWGG | 8 cut(s) | 235, 301, 795, 935, 993, 1034, 1364, 2010 |
| Eco24I | GRGCYC | 4 cut(s) | 508, 688, 1604, 2135 |
| Eco47I | GGWCC | 3 cut(s) | 1464, 1839, 2099 |
| Eco53kI | GAGCTC | 1 cut(s) | 686 |
| Eco57I | CTGAAG | 5 cut(s) | 30, 172, 1121, 1439, 1506 |
| Eco88I | CYCGRG | 1 cut(s) | 389 |
| Eco91I | GGTNACC | 1 cut(s) | 1262 |
| EcoICRI | GAGCTC | 1 cut(s) | 686 |
| EcoO109I | RGGNCCY | 1 cut(s) | 504 |
| EcoO65I | GGTNACC | 1 cut(s) | 1262 |
| EcoRI | GAATTC | 1 cut(s) | 1328 |
| EcoRII | CCWGG | 3 cut(s) | 904, 1438, 1747 |
| EcoT14I | CCWWGG | 8 cut(s) | 235, 301, 795, 935, 993, 1034, 1364, 2010 |
| EcoT38I | GRGCYC | 4 cut(s) | 508, 688, 1604, 2135 |
| ErhI | CCWWGG | 8 cut(s) | 235, 301, 795, 935, 993, 1034, 1364, 2010 |
| FaeI | CATG | 8 cut(s) | 40, 55, 590, 830, 862, 939, 1464, 1556 |
| FaqI | GGGAC | 2 cut(s) | 1450, 1546 |
| FatI | CATG | 8 cut(s) | 36, 51, 586, 826, 858, 935, 1460, 1552 |
| FauI | CCCGC | 2 cut(s) | 293, 578 |
| FbaI | TGATCA | 1 cut(s) | 70 |
| FblI | GTMKAC | 2 cut(s) | 753, 1287 |
| FokI | GGATG | 5 cut(s) | 622, 824, 1009, 1575, 1940 |
| FriOI | GRGCYC | 4 cut(s) | 508, 688, 1604, 2135 |
| FspBI | CTAG | 4 cut(s) | 435, 1047, 1473, 1754 |
| GlaI | GCGC | 4 cut(s) | 325, 634, 1548, 1572 |
| GsuI | CTGGAG | 1 cut(s) | 399 |
| HaeII | RGCGCY | 2 cut(s) | 327, 1550 |
| HaeIII | GGCC | 7 cut(s) | 331, 388, 506, 903, 1393, 1559, 2189 |
| HapII | CCGG | 5 cut(s) | 459, 508, 729, 891, 1560 |
| HhaI | GCGC | 4 cut(s) | 326, 635, 1549, 1573 |
| Hin1II | CATG | 8 cut(s) | 40, 55, 590, 830, 862, 939, 1464, 1556 |
| Hin6I | GCGC | 4 cut(s) | 324, 633, 1547, 1571 |
| HinP1I | GCGC | 4 cut(s) | 324, 633, 1547, 1571 |
| HincII | GTYRAC | 2 cut(s) | 121, 1372 |
| HindII | GTYRAC | 2 cut(s) | 121, 1372 |
| HindIII | AAGCTT | 1 cut(s) | 1787 |
| HinfI | GANTC | 8 cut(s) | 41, 218, 421, 455, 538, 1639, 1999, 2207 |
| HpaII | CCGG | 5 cut(s) | 459, 508, 729, 891, 1560 |
| HphI | GGTGA | 4 cut(s) | 65, 271, 359, 1274 |
| Hpy166II | GTNNAC | 8 cut(s) | 34, 121, 754, 778, 932, 1288, 1372, 2055 |
| Hpy188I | TCNGA | 9 cut(s) | 112, 211, 736, 980, 1222, 1419, 1486, 1927, 2214 |
| Hpy8I | GTNNAC | 8 cut(s) | 34, 121, 754, 778, 932, 1288, 1372, 2055 |
| HpyAV | CCTTC | 3 cut(s) | 73, 407, 2023 |
| HpyCH4III | ACNGT | 6 cut(s) | 116, 361, 1342, 1382, 1910, 1983 |
| HpyF3I | CTNAG | 6 cut(s) | 126, 210, 919, 1056, 2075, 2138 |
| Hsp92II | CATG | 8 cut(s) | 40, 55, 590, 830, 862, 939, 1464, 1556 |
| HspAI | GCGC | 4 cut(s) | 324, 633, 1547, 1571 |
| Kpn2I | TCCGGA | 1 cut(s) | 728 |
| KpnI | GGTACC | 1 cut(s) | 918 |
| Ksp22I | TGATCA | 1 cut(s) | 70 |
| Kzo9I | GATC | 8 cut(s) | 70, 259, 731, 967, 1442, 1489, 1616, 1744 |
| LguI | GCTCTTC | 2 cut(s) | 295, 481 |
| LmnI | GCTCC | 5 cut(s) | 236, 1190, 1599, 1712, 2035 |
| LweI | GCATC | 6 cut(s) | 644, 1466, 1597, 1663, 1753, 1918 |
| MaeI | CTAG | 4 cut(s) | 435, 1047, 1473, 1754 |
| MaeIII | GTNAC | 5 cut(s) | 355, 1262, 1511, 1910, 1988 |
| MalI | GATC | 8 cut(s) | 72, 261, 733, 969, 1444, 1491, 1618, 1746 |
| MbiI | CCGCTC | 1 cut(s) | 288 |
| MboI | GATC | 8 cut(s) | 70, 259, 731, 967, 1442, 1489, 1616, 1744 |
| MboII | GAAGA | 6 cut(s) | 36, 282, 468, 1114, 1499, 2129 |
| MflI | RGATCY | 1 cut(s) | 731 |
| MhlI | GDGCHC | 5 cut(s) | 508, 688, 934, 1604, 2135 |
| MlsI | TGGCCA | 1 cut(s) | 2189 |
| MluNI | TGGCCA | 1 cut(s) | 2189 |
| MlyI | GAGTC | 1 cut(s) | 415 |
| MmeI | TCCRAC | 1 cut(s) | 2123 |
| Mox20I | TGGCCA | 1 cut(s) | 2189 |
| MroI | TCCGGA | 1 cut(s) | 728 |
| MscI | TGGCCA | 1 cut(s) | 2189 |
| Msp20I | TGGCCA | 1 cut(s) | 2189 |
| MspA1I | CMGCKG | 3 cut(s) | 665, 884, 1250 |
| MspI | CCGG | 5 cut(s) | 459, 508, 729, 891, 1560 |
| MspR9I | CCNGG | 4 cut(s) | 508, 906, 1440, 1749 |
| Mva1269I | GAATGC | 1 cut(s) | 1171 |
| MvaI | CCWGG | 3 cut(s) | 906, 1440, 1749 |
| MvnI | CGCG | 1 cut(s) | 573 |
| NciI | CCSGG | 1 cut(s) | 508 |
| NcoI | CCATGG | 1 cut(s) | 935 |
| NdeII | GATC | 8 cut(s) | 70, 259, 731, 967, 1442, 1489, 1616, 1744 |
| NlaIII | CATG | 8 cut(s) | 40, 55, 590, 830, 862, 939, 1464, 1556 |
| NmuCI | GTSAC | 2 cut(s) | 355, 1262 |
| NspV | TTCGAA | 1 cut(s) | 338 |
| PagI | TCATGA | 1 cut(s) | 826 |
| PciSI | GCTCTTC | 2 cut(s) | 295, 481 |
| PctI | GAATGC | 1 cut(s) | 1171 |
| PfeI | GAWTC | 7 cut(s) | 41, 218, 455, 538, 1639, 1999, 2207 |
| PflFI | GACNNNGTC | 1 cut(s) | 116 |
| PfoI | TCCNGGA | 1 cut(s) | 1747 |
| Ple19I | CGATCG | 1 cut(s) | 970 |
| PleI | GAGTC | 1 cut(s) | 415 |
| PpsI | GAGTC | 1 cut(s) | 415 |
| PshBI | ATTAAT | 2 cut(s) | 1448, 1878 |
| PsiI | TTATAA | 1 cut(s) | 1848 |
| Psp124BI | GAGCTC | 1 cut(s) | 688 |
| Psp6I | CCWGG | 3 cut(s) | 904, 1438, 1747 |
| PspEI | GGTNACC | 1 cut(s) | 1262 |
| PspGI | CCWGG | 3 cut(s) | 904, 1438, 1747 |
| PspOMI | GGGCCC | 1 cut(s) | 504 |
| PspPI | GGNCC | 8 cut(s) | 387, 504, 505, 902, 1391, 1464, 1839, 2099 |
| PstI | CTGCAG | 1 cut(s) | 889 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1193, 1484 |
| PsuI | RGATCY | 1 cut(s) | 731 |
| PsyI | GACNNNGTC | 1 cut(s) | 116 |
| PvuI | CGATCG | 1 cut(s) | 970 |
| PvuII | CAGCTG | 2 cut(s) | 665, 884 |
| RsaI | GTAC | 6 cut(s) | 142, 661, 701, 916, 1351, 2081 |
| RsaNI | GTAC | 6 cut(s) | 141, 660, 700, 915, 1350, 2080 |
| SacI | GAGCTC | 1 cut(s) | 688 |
| SapI | GCTCTTC | 2 cut(s) | 295, 481 |
| Sau3AI | GATC | 8 cut(s) | 70, 259, 731, 967, 1442, 1489, 1616, 1744 |
| Sau96I | GGNCC | 8 cut(s) | 387, 504, 505, 902, 1391, 1464, 1839, 2099 |
| ScaI | AGTACT | 1 cut(s) | 142 |
| SchI | GAGTC | 1 cut(s) | 415 |
| ScrFI | CCNGG | 4 cut(s) | 508, 906, 1440, 1749 |
| SduI | GDGCHC | 5 cut(s) | 508, 688, 934, 1604, 2135 |
| SfaNI | GCATC | 6 cut(s) | 644, 1466, 1597, 1663, 1753, 1918 |
| SfcI | CTRYAG | 2 cut(s) | 666, 885 |
| SfuI | TTCGAA | 1 cut(s) | 338 |
| SinI | GGWCC | 3 cut(s) | 1464, 1839, 2099 |
| SpeI | ACTAGT | 2 cut(s) | 1472, 1753 |
| SsiI | CCGC | 4 cut(s) | 286, 571, 1250, 1607 |
| SspI | AATATT | 1 cut(s) | 818 |
| SspMI | CTAG | 4 cut(s) | 435, 1047, 1473, 1754 |
| SstI | GAGCTC | 1 cut(s) | 688 |
| StyD4I | CCNGG | 4 cut(s) | 506, 904, 1438, 1747 |
| StyI | CCWWGG | 8 cut(s) | 235, 301, 795, 935, 993, 1034, 1364, 2010 |
| TaaI | ACNGT | 6 cut(s) | 116, 361, 1342, 1382, 1910, 1983 |
| TaqI | TCGA | 3 cut(s) | 338, 688, 966 |
| TatI | WGTACW | 2 cut(s) | 140, 699 |
| TauI | GCSGC | 2 cut(s) | 1253, 1610 |
| TfiI | GAWTC | 7 cut(s) | 41, 218, 455, 538, 1639, 1999, 2207 |
| TscAI | CASTG | 3 cut(s) | 196, 382, 948 |
| TseFI | GTSAC | 2 cut(s) | 355, 1262 |
| Tsp45I | GTSAC | 2 cut(s) | 355, 1262 |
| TspGWI | ACGGA | 1 cut(s) | 1695 |
| TspRI | CASTG | 3 cut(s) | 196, 382, 948 |
| Tth111I | GACNNNGTC | 1 cut(s) | 116 |
| VneI | GTGCAC | 1 cut(s) | 930 |
| VpaK11BI | GGWCC | 3 cut(s) | 1464, 1839, 2099 |
| VspI | ATTAAT | 2 cut(s) | 1448, 1878 |
| XapI | RAATTY | 4 cut(s) | 870, 984, 1328, 1921 |
| XbaI | TCTAGA | 2 cut(s) | 434, 1046 |
| XmiI | GTMKAC | 2 cut(s) | 753, 1287 |
| XspI | CTAG | 4 cut(s) | 435, 1047, 1473, 1754 |
| ZrmI | AGTACT | 1 cut(s) | 142 |