Rmu_sc0003116.1_g000043

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003116.1
OG0001268
Physical Location & Seq
Forward (+)
235785 .. 238103
2319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003116.1_g000043.1.cds

Sequence Viewer

Length: 2319 bp
atggggatcaattatggtgttccttttccttgcattttctatgctcttcagctttttctgttggctctccatgttaacacttctttggcagagagatccacttatattgtccacatggacaagtctctcatgcccaagttcttcactagccatcaacattggtatacctccgttgttgattccttcaaaactgaatttcaaacctcatcagatagccacagcttctcaccatcccttttatacacctatgacaatgcattccatggtttttctgcagttctgtctctagatgaactcgagaccctaaaaaagtgcccgggttttgtttcagcttataatgacaaaactgttacacttgacaccacccataccagtgaattcctctccctcaacccttctactggcctcttgccagcctcaaattatggagaagacatcatcattggtctcattgataccggtgtctggccagagagcgagagcttcaaagatgatggtctaaccaaagacattccaactaggtggaagggaacatgtgaggttggacaagagttcaatgcctctttatgtaacaaaaagttgattggagctagatactttaacaagggtgtgctcgctgcaaaccccggcatcacacttagcatgaactctgctagagacactgaaggccatgggacacatacatcttccactgctgcagggaactttgtgaatggagcatcttattttggttacgctaaaggaacagccagaggtgtcgcaccacgctcaagggttgcaatgtacaaagtcctttgggatgtaggtaaagggcgttacgcgtctgatgttctagctggtatagaccaagccattgctgatggtgtggatgtaatttccatttctatggggtttgatgaaacgccactctatgaagatcctatagcaatagcatcttttgcaaccatggaaaagaaggtggtggtttcaacttcagccggaaacttcggccgaggtggccttggatggttgcacaatggtatcccttgggtcttgactgttgcagctggcacaatagaccgctcctttggcggaacattaactcttgataatggtgcaaccattactgggtttaccttgttaccagcaaatgccatagtagaaaaggttccactggtttacaacaagaccttctcagcttgcaactcgacaaaggtattatcaaatgctccagagggagtcatcatcatatgtgacgagagtcatgctatttggggtcagatatctaagttcattggatcacatgtgcttggggctgtctttattggaaaatatcctgaaatatttgagttgggaggtgtgacatttcctctcgtagtggtaagtccagaagatgcactgggtttaatcaagtatgttacaactagtgttaaaccttctgtgagcatgaaattccaacaaacatttgttggcacaaagcctgcaccggctgcttcattttacacttcaagaggtccttcatcaagttatccgggtatcttgaagcctgacataatggcaccggggtcattagttttggctgcatggcctccgaatgttgtagcaggccaaattggatcaaatgtgatgttaccaagtgactacaatttgatatctggcacatccatggcttgtcctcatgcttcaggtgtagcggctttattgaaaggtgcacacccggaatggagtgcagctgccattaggtctgctttggtcaccacagccaaccccttagacaatactcagaatctgattcgtgacaatggtgacaatttcaactttgcttcacctctagctatgggagcaggccagattgatcctaatagagcacttgaacccggtttgatatatgatgcaaccccacaagattatgtcaatctcttgtgctccacaaattttacccataaacaaatcttagccatcactagatcacgtgcctatgcttgttccaacccatcttctgatctgaactatccctccttcattgctttgtatgatcgtcatcatcacacaaggaaactacgggtccaaaattttcagagaatagtaacaaatgtgggagatggtgcagctaagtacaaggcttcagtgacagctccaaagggttctacagtaacagtgtcaccggagacattgttcttttcagagacatatgagaagcgaagctacactcttactataaattacaagggagaaaagaaaggaaatgtgtcatttggtgaacttgtttgggtggaagaaaatggaaagtacaatgtcaggagccccattgtagtgtcacctcttgtttcagtgtcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

772

Amino Acids

83.1

Weight (kDa)

6.53

Isoelectric Point (pI)

29.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
AccB1I GGYRCC 1 cut(s) 1537
AccBSI CCGCTC 1 cut(s) 1062
AccI GTMKAC 1 cut(s) 164
AccII CGCG 1 cut(s) 821
AciI CCGC 3 cut(s) 1060, 1071, 1673
AclWI GGATC 6 cut(s) 14, 90, 911, 1285, 1603, 1829
AcoI YGGCCR 2 cut(s) 467, 988
AcsI RAATTY 5 cut(s) 194, 377, 1430, 1912, 2050
AcuI CTGAAG 5 cut(s) 32, 684, 957, 1647, 2088
AcvI CACGTG 1 cut(s) 1952
AfaI GTAC 3 cut(s) 785, 2096, 2270
AfiI CCNNNNNNNGG 3 cut(s) 401, 465, 1107
AflIII ACRYGT 3 cut(s) 533, 819, 1282
AgeI ACCGGT 1 cut(s) 458
AhlI ACTAGT 1 cut(s) 1403
AloI GAACNNNNNNTCC 4 cut(s) 1979, 2011, 2138, 2170
Alw21I GWGCWC 4 cut(s) 615, 1693, 1849, 1907
Alw26I GTCTC 7 cut(s) 129, 288, 293, 452, 651, 2141, 2159
Alw44I GTGCAC 1 cut(s) 1689
AlwI GGATC 6 cut(s) 14, 90, 911, 1285, 1603, 1829
AlwNI CAGNNNCTG 1 cut(s) 1768
Ama87I CYCGRG 2 cut(s) 296, 316
AoxI GGCC 8 cut(s) 403, 467, 667, 988, 997, 1565, 1585, 1825
ApaLI GTGCAC 1 cut(s) 1689
ApeKI GCWGC 8 cut(s) 617, 695, 1043, 1469, 1559, 1709, 1712, 2087
ApoI RAATTY 5 cut(s) 194, 377, 1430, 1912, 2050
ArsI GACNNNNNNTTYG 2 cut(s) 1049, 1081
AsiGI ACCGGT 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 1146
AspS9I GGNCC 2 cut(s) 1493, 2044
AsuC2I CCSGG 7 cut(s) 317, 318, 627, 1512, 1542, 1697, 1857
AsuHPI GGTGA 7 cut(s) 219, 1726, 1796, 1797, 2133, 2249, 2289
AvaI CYCGRG 2 cut(s) 296, 316
AvaII GGWCC 2 cut(s) 1493, 2044
BaeGI GKGCMC 2 cut(s) 317, 1693
BaeI ACNNNNGTAYC 4 cut(s) 447, 480, 1003, 1036
BalI TGGCCA 1 cut(s) 469
BanI GGYRCC 1 cut(s) 1537
BanII GRGCYC 1 cut(s) 2285
BarI GAAGNNNNNNTAC 2 cut(s) 2168, 2200
BbrPI CACGTG 1 cut(s) 1952
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 4 cut(s) 615, 1693, 1849, 1907
BbvI GCAGC 8 cut(s) 604, 682, 1055, 1456, 1546, 1699, 1721, 2099
BccI CCATC 8 cut(s) 159, 238, 488, 854, 999, 1946, 1981, 2075
BcgI CGANNNNNNTGC 2 cut(s) 1226, 1260
BciVI GTATCC 1 cut(s) 1031
BcnI CCSGG 7 cut(s) 317, 318, 627, 1512, 1542, 1697, 1857
BcoDI GTCTC 7 cut(s) 129, 288, 293, 452, 651, 2141, 2159
BcuI ACTAGT 1 cut(s) 1403
BfaI CTAG 9 cut(s) 147, 287, 519, 591, 654, 833, 1404, 1811, 1944
BfmI CTRYAG 4 cut(s) 273, 696, 921, 2127
BfuI GTATCC 1 cut(s) 1031
BglI GCCNNNNNGGC 1 cut(s) 996
BisI GCNGC 9 cut(s) 618, 696, 1044, 1470, 1560, 1674, 1710, 1713, 2088
BlsI GCNGC 9 cut(s) 619, 697, 1045, 1471, 1561, 1675, 1711, 1714, 2089
Bme1390I CCNGG 7 cut(s) 317, 318, 627, 1512, 1542, 1697, 1857
Bme18I GGWCC 2 cut(s) 1493, 2044
BmeT110I CYCGRG 2 cut(s) 296, 316
BmgT120I GGNCC 2 cut(s) 1493, 2044
BmiI GGNNCC 4 cut(s) 1149, 1539, 2045, 2282
BmrFI CCNGG 7 cut(s) 317, 318, 627, 1512, 1542, 1697, 1857
BmrI ACTGGG 2 cut(s) 1116, 1388
BmsI GCATC 5 cut(s) 639, 728, 941, 1363, 1861
BmuI ACTGGG 2 cut(s) 1116, 1388
BoxI GACNNNNGTC 1 cut(s) 1239
BpiI GAAGAC 1 cut(s) 438
BpmI CTGGAG 1 cut(s) 1194
BpuEI CTTGAG 1 cut(s) 754
BpuMI CCSGG 7 cut(s) 317, 318, 627, 1512, 1542, 1697, 1857
BsaAI YACGTR 1 cut(s) 1952
BsaBI GATNNNNATC 1 cut(s) 2019
BsaI GGTCTC 2 cut(s) 293, 452
BsaWI WCCGGW 2 cut(s) 458, 2143
BsaXI ACNNNNNCTCC 4 cut(s) 1979, 2009, 2138, 2168
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 465, 1107
Bse118I RCCGGY 2 cut(s) 458, 1465
Bse1I ACTGG 5 cut(s) 372, 406, 1111, 1158, 1383
Bse3DI GCAATG 3 cut(s) 786, 852, 2001
Bse8I GATNNNNATC 1 cut(s) 2019
BseGI GGATG 5 cut(s) 230, 805, 874, 1010, 1640
BseJI GATNNNNATC 1 cut(s) 2019
BseLI CCNNNNNNNGG 3 cut(s) 401, 465, 1107
BseMI GCAATG 3 cut(s) 786, 852, 2001
BseMII CTCAG 2 cut(s) 1188, 1775
BseNI ACTGG 5 cut(s) 372, 406, 1111, 1158, 1383
BseSI GKGCMC 2 cut(s) 317, 1693
BseX3I CGGCCG 1 cut(s) 988
BseXI GCAGC 8 cut(s) 604, 682, 1055, 1456, 1546, 1699, 1721, 2099
BsgI GTGCAG 3 cut(s) 1446, 1728, 2106
Bsh1236I CGCG 1 cut(s) 821
Bsh1285I CGRYCG 1 cut(s) 991
BshFI GGCC 8 cut(s) 405, 469, 669, 990, 999, 1567, 1587, 1827
BshNI GGYRCC 1 cut(s) 1537
BshTI ACCGGT 1 cut(s) 458
BsiEI CGRYCG 1 cut(s) 991
BsiHKAI GWGCWC 4 cut(s) 615, 1693, 1849, 1907
BsiHKCI CYCGRG 2 cut(s) 296, 316
BslFI GGGAC 1 cut(s) 688
BslI CCNNNNNNNGG 3 cut(s) 401, 465, 1107
BsmAI GTCTC 7 cut(s) 129, 288, 293, 452, 651, 2141, 2159
BsmFI GGGAC 1 cut(s) 688
BsmI GAATGC 1 cut(s) 257
BsnI GGCC 8 cut(s) 405, 469, 669, 990, 999, 1567, 1587, 1827
Bso31I GGTCTC 2 cut(s) 293, 452
BsoBI CYCGRG 2 cut(s) 296, 316
Bsp1286I GDGCHC 6 cut(s) 317, 615, 1693, 1849, 1907, 2285
Bsp1407I TGTACA 1 cut(s) 783
Bsp143I GATC 9 cut(s) 6, 95, 916, 1277, 1595, 1834, 1946, 1981, 2014
Bsp19I CCATGG 4 cut(s) 262, 670, 945, 1644
BspACI CCGC 3 cut(s) 1060, 1071, 1673
BspANI GGCC 8 cut(s) 405, 469, 669, 990, 999, 1567, 1587, 1827
BspCNI CTCAG 2 cut(s) 1187, 1774
BspFNI CGCG 1 cut(s) 821
BspLI GGNNCC 4 cut(s) 1149, 1539, 2045, 2282
BspMAI CTGCAG 2 cut(s) 277, 700
BspPI GGATC 6 cut(s) 14, 90, 911, 1285, 1603, 1829
BspQI GCTCTTC 1 cut(s) 51
BspT107I GGYRCC 1 cut(s) 1537
BspTNI GGTCTC 2 cut(s) 293, 452
BsrBI CCGCTC 1 cut(s) 1062
BsrDI GCAATG 3 cut(s) 786, 852, 2001
BsrFI RCCGGY 2 cut(s) 458, 1465
BsrGI TGTACA 1 cut(s) 783
BsrI ACTGG 5 cut(s) 372, 406, 1111, 1158, 1383
BssAI RCCGGY 2 cut(s) 458, 1465
BssMI GATC 9 cut(s) 6, 95, 916, 1277, 1595, 1834, 1946, 1981, 2014
BssNAI GTATAC 1 cut(s) 165
BssT1I CCWWGG 6 cut(s) 262, 670, 945, 1000, 1025, 1644
Bst1107I GTATAC 1 cut(s) 165
Bst4CI ACNGT 4 cut(s) 349, 1039, 2131, 2137
Bst6I CTCTTC 1 cut(s) 51
BstAPI GCANNNNNTGC 2 cut(s) 938, 1469
BstAUI TGTACA 1 cut(s) 783
BstBAI YACGTR 1 cut(s) 1952
BstC8I GCNNGC 7 cut(s) 414, 615, 1048, 1180, 1461, 1585, 1825
BstDEI CTNAG 7 cut(s) 638, 1174, 1266, 1750, 1761, 1933, 2091
BstDSI CCRYGG 4 cut(s) 262, 670, 945, 1644
BstEII GGTNACC 1 cut(s) 1732
BstF5I GGATG 5 cut(s) 230, 805, 874, 1010, 1640
BstFNI CGCG 1 cut(s) 821
BstKTI GATC 9 cut(s) 9, 98, 919, 1280, 1598, 1837, 1949, 1984, 2017
BstMAI GTCTC 7 cut(s) 129, 288, 293, 452, 651, 2141, 2159
BstMBI GATC 9 cut(s) 6, 95, 916, 1277, 1595, 1834, 1946, 1981, 2014
BstMCI CGRYCG 1 cut(s) 991
BstMWI GCNNNNNNNGC 5 cut(s) 938, 996, 1068, 1469, 1820
BstNSI RCATGY 2 cut(s) 537, 1286
BstPAI GACNNNNGTC 1 cut(s) 1239
BstPI GGTNACC 1 cut(s) 1732
BstSCI CCNGG 7 cut(s) 315, 316, 625, 1510, 1540, 1695, 1855
BstSFI CTRYAG 4 cut(s) 273, 696, 921, 2127
BstSLI GKGCMC 2 cut(s) 317, 1693
BstUI CGCG 1 cut(s) 821
BstV1I GCAGC 8 cut(s) 604, 682, 1055, 1456, 1546, 1699, 1721, 2099
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 2 cut(s) 95, 916
BstXI CCANNNNNNTGG 2 cut(s) 522, 886
BstYI RGATCY 2 cut(s) 95, 916
BstZ17I GTATAC 1 cut(s) 165
BstZI CGGCCG 1 cut(s) 988
BsuI GTATCC 1 cut(s) 1031
BsuRI GGCC 8 cut(s) 405, 469, 669, 990, 999, 1567, 1587, 1827
BtgI CCRYGG 4 cut(s) 262, 670, 945, 1644
BtsCI GGATG 5 cut(s) 230, 805, 874, 1010, 1640
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 8 cut(s) 379, 660, 690, 1151, 1376, 2112, 2142, 2316
Cac8I GCNNGC 7 cut(s) 414, 615, 1048, 1180, 1461, 1585, 1825
CaiI CAGNNNCTG 1 cut(s) 1768
Cfr10I RCCGGY 2 cut(s) 458, 1465
Cfr13I GGNCC 2 cut(s) 1493, 2044
Cfr9I CCCGGG 1 cut(s) 316
CseI GACGC 1 cut(s) 810
Csp6I GTAC 3 cut(s) 784, 2095, 2269
CspAI ACCGGT 1 cut(s) 458
CspCI CAANNNNNGTGG 2 cut(s) 88, 123
CviQI GTAC 3 cut(s) 784, 2095, 2269
DdeI CTNAG 7 cut(s) 638, 1174, 1266, 1750, 1761, 1933, 2091
DpnI GATC 9 cut(s) 8, 97, 918, 1279, 1597, 1836, 1948, 1983, 2016
DpnII GATC 9 cut(s) 6, 95, 916, 1277, 1595, 1834, 1946, 1981, 2014
EaeI YGGCCR 2 cut(s) 467, 988
EagI CGGCCG 1 cut(s) 988
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
EciI GGCGGA 1 cut(s) 1086
EclXI CGGCCG 1 cut(s) 988
Eco130I CCWWGG 6 cut(s) 262, 670, 945, 1000, 1025, 1644
Eco24I GRGCYC 1 cut(s) 2285
Eco31I GGTCTC 2 cut(s) 293, 452
Eco32I GATATC 2 cut(s) 1263, 1632
Eco47I GGWCC 2 cut(s) 1493, 2044
Eco52I CGGCCG 1 cut(s) 988
Eco57I CTGAAG 5 cut(s) 32, 684, 957, 1647, 2088
Eco72I CACGTG 1 cut(s) 1952
Eco88I CYCGRG 2 cut(s) 296, 316
Eco91I GGTNACC 1 cut(s) 1732
EcoO109I RGGNCCY 1 cut(s) 1493
EcoO65I GGTNACC 1 cut(s) 1732
EcoRI GAATTC 1 cut(s) 377
EcoRV GATATC 2 cut(s) 1263, 1632
EcoT14I CCWWGG 6 cut(s) 262, 670, 945, 1000, 1025, 1644
EcoT22I ATGCAT 1 cut(s) 259
EcoT38I GRGCYC 1 cut(s) 2285
ErhI CCWWGG 6 cut(s) 262, 670, 945, 1000, 1025, 1644
FalI AAGNNNNNCTT 2 cut(s) 1480, 1512
FaqI GGGAC 1 cut(s) 688
FauNDI CATATG 2 cut(s) 1229, 2170
FblI GTMKAC 1 cut(s) 164
Fnu4HI GCNGC 9 cut(s) 618, 696, 1044, 1470, 1560, 1674, 1710, 1713, 2088
FokI GGATG 5 cut(s) 217, 812, 881, 1017, 1627
FriOI GRGCYC 1 cut(s) 2285
Fsp4HI GCNGC 9 cut(s) 618, 696, 1044, 1470, 1560, 1674, 1710, 1713, 2088
FspBI CTAG 9 cut(s) 147, 287, 519, 591, 654, 833, 1404, 1811, 1944
GluI GCNGC 9 cut(s) 618, 696, 1044, 1470, 1560, 1674, 1710, 1713, 2088
GsuI CTGGAG 1 cut(s) 1194
HaeIII GGCC 8 cut(s) 405, 469, 669, 990, 999, 1567, 1587, 1827
HgaI GACGC 1 cut(s) 810
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 5 cut(s) 179, 1218, 1240, 1765, 1771
HpaI GTTAAC 1 cut(s) 76
HphI GGTGA 7 cut(s) 219, 1726, 1796, 1797, 2133, 2249, 2289
Hpy166II GTNNAC 7 cut(s) 76, 112, 165, 1113, 1159, 1691, 2240
Hpy8I GTNNAC 7 cut(s) 76, 112, 165, 1113, 1159, 1691, 2240
HpyAV CCTTC 9 cut(s) 193, 405, 520, 659, 949, 1180, 1425, 1506, 2008
HpyCH4III ACNGT 4 cut(s) 349, 1039, 2131, 2137
HpyCH4IV ACGT 1 cut(s) 1951
HpyF10VI GCNNNNNNNGC 5 cut(s) 938, 996, 1068, 1469, 1820
HpyF3I CTNAG 7 cut(s) 638, 1174, 1266, 1750, 1761, 1933, 2091
HpySE526I ACGT 1 cut(s) 1951
KspAI GTTAAC 1 cut(s) 76
Kzo9I GATC 9 cut(s) 6, 95, 916, 1277, 1595, 1834, 1946, 1981, 2014
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 8 cut(s) 587, 716, 1067, 1213, 1820, 1910, 2119, 2280
Lsp1109I GCAGC 8 cut(s) 604, 682, 1055, 1456, 1546, 1699, 1721, 2099
LweI GCATC 5 cut(s) 639, 728, 941, 1363, 1861
MaeI CTAG 9 cut(s) 147, 287, 519, 591, 654, 833, 1404, 1811, 1944
MaeII ACGT 1 cut(s) 1951
MalI GATC 9 cut(s) 8, 97, 918, 1279, 1597, 1836, 1948, 1983, 2016
MbiI CCGCTC 1 cut(s) 1062
MboI GATC 9 cut(s) 6, 95, 916, 1277, 1595, 1834, 1946, 1981, 2014
MboII GAAGA 8 cut(s) 38, 133, 443, 678, 926, 1382, 1968, 2267
MflI RGATCY 2 cut(s) 95, 916
MhlI GDGCHC 6 cut(s) 317, 615, 1693, 1849, 1907, 2285
MlsI TGGCCA 1 cut(s) 469
MluI ACGCGT 1 cut(s) 819
MluNI TGGCCA 1 cut(s) 469
MlyI GAGTC 2 cut(s) 1227, 1249
MmeI TCCRAC 4 cut(s) 523, 539, 1459, 1992
Mox20I TGGCCA 1 cut(s) 469
Mph1103I ATGCAT 1 cut(s) 259
MroXI GAANNNNTTC 1 cut(s) 1146
MscI TGGCCA 1 cut(s) 469
MseI TTAA 6 cut(s) 75, 600, 1079, 1385, 1410, 2317
MslI CAYNNNNRTG 4 cut(s) 372, 884, 1643, 2291
Msp20I TGGCCA 1 cut(s) 469
MspA1I CMGCKG 2 cut(s) 1046, 1712
MspR9I CCNGG 7 cut(s) 317, 318, 627, 1512, 1542, 1697, 1857
Mva1269I GAATGC 1 cut(s) 257
MvnI CGCG 1 cut(s) 821
MwoI GCNNNNNNNGC 5 cut(s) 938, 996, 1068, 1469, 1820
NciI CCSGG 7 cut(s) 317, 318, 627, 1512, 1542, 1697, 1857
NcoI CCATGG 4 cut(s) 262, 670, 945, 1644
NdeI CATATG 2 cut(s) 1229, 2170
NdeII GATC 9 cut(s) 6, 95, 916, 1277, 1595, 1834, 1946, 1981, 2014
NlaIV GGNNCC 4 cut(s) 1149, 1539, 2045, 2282
NmeAIII GCCGAG 1 cut(s) 1016
NmuCI GTSAC 9 cut(s) 1232, 1339, 1616, 1732, 1775, 1784, 2107, 2139, 2295
NsiI ATGCAT 1 cut(s) 259
NspI RCATGY 2 cut(s) 537, 1286
PaeR7I CTCGAG 1 cut(s) 296
PciI ACATGT 2 cut(s) 533, 1282
PciSI GCTCTTC 1 cut(s) 51
PctI GAATGC 1 cut(s) 257
PdmI GAANNNNTTC 1 cut(s) 1146
PfeI GAWTC 3 cut(s) 179, 1765, 1771
PinAI ACCGGT 1 cut(s) 458
PkrI GCNGC 9 cut(s) 619, 697, 1045, 1471, 1561, 1675, 1711, 1714, 2089
PleI GAGTC 2 cut(s) 1226, 1248
PmaCI CACGTG 1 cut(s) 1952
PmlI CACGTG 1 cut(s) 1952
PpsI GAGTC 2 cut(s) 1226, 1248
Ppu21I YACGTR 1 cut(s) 1952
PpuMI RGGWCCY 1 cut(s) 1493
PscI ACATGT 2 cut(s) 533, 1282
PshAI GACNNNNGTC 1 cut(s) 1239
PsiI TTATAA 1 cut(s) 336
Psp5II RGGWCCY 1 cut(s) 1493
PspCI CACGTG 1 cut(s) 1952
PspEI GGTNACC 1 cut(s) 1732
PspN4I GGNNCC 4 cut(s) 1149, 1539, 2045, 2282
PspPI GGNCC 2 cut(s) 1493, 2044
PspPPI RGGWCCY 1 cut(s) 1493
PsrI GAACNNNNNNTAC 2 cut(s) 1131, 1163
PstI CTGCAG 2 cut(s) 277, 700
PstNI CAGNNNCTG 1 cut(s) 1768
PsuI RGATCY 2 cut(s) 95, 916
PvuII CAGCTG 2 cut(s) 1046, 1712
RsaI GTAC 3 cut(s) 785, 2096, 2270
RsaNI GTAC 3 cut(s) 784, 2095, 2269
RseI CAYNNNNRTG 4 cut(s) 372, 884, 1643, 2291
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 6 cut(s) 75, 600, 1079, 1385, 1410, 2317
SatI GCNGC 9 cut(s) 618, 696, 1044, 1470, 1560, 1674, 1710, 1713, 2088
Sau3AI GATC 9 cut(s) 6, 95, 916, 1277, 1595, 1834, 1946, 1981, 2014
Sau96I GGNCC 2 cut(s) 1493, 2044
SchI GAGTC 2 cut(s) 1227, 1249
ScrFI CCNGG 7 cut(s) 317, 318, 627, 1512, 1542, 1697, 1857
SduI GDGCHC 6 cut(s) 317, 615, 1693, 1849, 1907, 2285
SfaNI GCATC 5 cut(s) 639, 728, 941, 1363, 1861
SfcI CTRYAG 4 cut(s) 273, 696, 921, 2127
SfiI GGCCNNNNNGGCC 1 cut(s) 996
Sfr274I CTCGAG 1 cut(s) 296
SinI GGWCC 2 cut(s) 1493, 2044
SlaI CTCGAG 1 cut(s) 296
SmaI CCCGGG 1 cut(s) 318
SmiMI CAYNNNNRTG 4 cut(s) 372, 884, 1643, 2291
SmlI CTYRAG 2 cut(s) 296, 769
SmoI CTYRAG 2 cut(s) 296, 769
SpeI ACTAGT 1 cut(s) 1403
SsiI CCGC 3 cut(s) 1060, 1071, 1673
SspI AATATT 1 cut(s) 1323
SspMI CTAG 9 cut(s) 147, 287, 519, 591, 654, 833, 1404, 1811, 1944
StyD4I CCNGG 7 cut(s) 315, 316, 625, 1510, 1540, 1695, 1855
StyI CCWWGG 6 cut(s) 262, 670, 945, 1000, 1025, 1644
TaaI ACNGT 4 cut(s) 349, 1039, 2131, 2137
TaiI ACGT 1 cut(s) 1954
TaqI TCGA 2 cut(s) 297, 1187
TatI WGTACW 3 cut(s) 783, 2094, 2268
TauI GCSGC 1 cut(s) 1676
TfiI GAWTC 3 cut(s) 179, 1765, 1771
Tru1I TTAA 6 cut(s) 75, 600, 1079, 1385, 1410, 2317
Tru9I TTAA 6 cut(s) 75, 600, 1079, 1385, 1410, 2317
TscAI CASTG 8 cut(s) 379, 667, 697, 1158, 1383, 2112, 2142, 2316
TseFI GTSAC 9 cut(s) 1232, 1339, 1616, 1732, 1775, 1784, 2107, 2139, 2295
TseI GCWGC 8 cut(s) 617, 695, 1043, 1469, 1559, 1709, 1712, 2087
Tsp45I GTSAC 9 cut(s) 1232, 1339, 1616, 1732, 1775, 1784, 2107, 2139, 2295
TspDTI ATGAA 9 cut(s) 306, 659, 912, 927, 1261, 1442, 1464, 1488, 1990
TspGWI ACGGA 1 cut(s) 160
TspMI CCCGGG 1 cut(s) 316
TspRI CASTG 8 cut(s) 379, 667, 697, 1158, 1383, 2112, 2142, 2316
VneI GTGCAC 1 cut(s) 1689
VpaK11BI GGWCC 2 cut(s) 1493, 2044
XapI RAATTY 5 cut(s) 194, 377, 1430, 1912, 2050
XbaI TCTAGA 1 cut(s) 286
XceI RCATGY 2 cut(s) 537, 1286
XhoI CTCGAG 1 cut(s) 296
XmaI CCCGGG 1 cut(s) 316
XmiI GTMKAC 1 cut(s) 164
XmnI GAANNNNTTC 1 cut(s) 1146
XspI CTAG 9 cut(s) 147, 287, 519, 591, 654, 833, 1404, 1811, 1944
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.