MD08G1098100.v1.1

subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001268
Physical Location & Seq
Forward (+)
8237213 .. 8239531
2319 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1098100.v1.1.491

Sequence Viewer

Length: 2319 bp
ATGTCTAGTTACTACCATGTTTTGTATTTCTGGCTTTCCATTACCACCATCTCACACCTCGCAATTGTCTCAACCTTTGCTCAATCCCAAAACTATATCATCCACATGGATTCATCAATGATGCCTAAAGCCTTTTCTGATCATCATTCATGGTACGTGGCAACATTACACTCCACATTAGGCAAGTTTACGCCTATCACCACCGGGACCTCCTCCTCCTCTGCCCTCTCTTCCAAACTCATTTATAGTTATACTCATGTCATGAATGGTTTCAGTGCGAGTCTCACTGCTTCCGAGCTTGAGGCCTTAAAAAGCTCTCTAGGCTACATTTCTTCAGTGAAAGACTTGCCGGTAATGAAAGACACAACTCACTCCTCTCAATTTCTTGGCCTCAACTCCAGATCCGGAGCATGGCCGGTTTCGAATTACGGCAAGGATGTGATCATTGGGTTGGTGGACACGGGTGTTTGGCCAGAAAGTGAAAGCTTTAACGAAGATGGGATGTCAGAAATCCCACCAAGATGGAAAGGAGAATGTGAAGGTGGCACCCAATTCAATTCCTCATTGTGCAACAAAAAGTTGATTGGGGCAAAGTTCTTTAACAAAGGGCTCATTGCCCAAAATCCCAATCTGACCATTGAAGTGAATTCAACAAGGGATACTGAAGGGCATGGTACCCACACCTCGTCTACTGCCGCAGGAAATTACGTGGCAGGGGCATCTTTTTTTGGTTACGCCCCAGGAGTTGCCAGCGGCATGGCTCCTAGGGCGCATGTGGCCATGTACAAGGCGCTTTGGGATGAGGGAGCATTGTCGTCGGATATAATTGCTGCCATTGAGCAAGCAATTATAGACGGAGTGGATGTTTTGTCTTTGTCATTTGGGTTAGATGGTGTTGCTTTGTATGAGGATCCTGTTGCCATAGCAACATTTTCTGCCTTGGAGAGAGGTGTTTTTGTGTCCACGTCTGCGGGCAATGAAGGACCTTTCTTTGCTACCTTGCACAATGGTATCCCTTGGGTTCTGACGGTTGCAGCTGGTACAATTGACCGGGAATTCGAAGGAACTGAGCATCTTGGAAATGGAAACTCTGCCACGGGGTCTACTCTCTATCCCGGAAATTCATCTTCAACCCAATTTCCGGTTGTTTTCTTTGATGCGTGTAACAATACGAAAACATTAAAGCAGGTGGGGAAGAAGATCGTCGTGTGCCAGGACAAAAATGATACGCTGAACGATCAAGTTTATAATGTACGCAGCGCAAATGTTGCCGGAGGTATCTTCATTACCAACAACACAGATTTGGAGTTGTTCATCCAAAGCTCTTTTCCCGCAATCTTTCTGAGTCCAAAGGAAGGTGAAGTGATCAAAGGTTACATCAAGAGCAACTCACAGCCAAAAGCAAGCTTGGAGTTTCAAAAAACACTTCTTGGTGCTAAACCAGCACCAAGTGTTACTAGCTACTCGTCTAGAGGGCCGTCCCCTAGCTTCCCGTGGACCCTGAAGCCAGATATTGTGGCTCCAGGGTCGTTAGTCCTGGCTGCATGGCCTCAAAATATCTCCGCAGCCGTGGTGGGCAAGAAAGACTTGTTTAGTAATTTTAATCTACTGTCAGGGACATCCATGTCGTGCCCCCATGCAGCTGGAATAGCTGCGCTTCTGAAAGGTGCGCACCCCGAATGGAGCCCTGCTGCTATTAGATCCGCCATGATGACCACATCAGACATATTGGATAACACCGGTAGCCCCATAAAAGACATTGGATACGCTAACCAACCAGCTAGTCCTCTAGCCATCGGAGCCGGGCATGTCAACCCTAACAAGGCCCTAGACCCCGGGCTCCTTTACGATGTGAATATTGACGACTACGTTAACCTTCTTTGTGCACTCAACTACACCAACAAGCAAATCCAAACCATCACTAAATCAGCCTCGAACAACTGTTCAACTCCTTCCTTAGATCTTAACTACCCCTCTTTCATAGCATTCTTCAATTCAAATGACTCAAATTCGGACGTACAATCTACTCATGAATTCCGGAGGACGGTGGCCAACATAGGAAAGGGACTGTCGACCTACGTTGCGAGCGTGACACCATTGAAAGGGTTTGTGGTTGGAGTCGTTCCGAAGAAGTTGGAGTTCAAGGAGGAGGGTGAGAAGCTGAGCTTTGTGCTGAGTATAAAAGGACCAAGAGTAATGAAAGAGGCAGTGGTTTTTGGTTATCTGAGTTGGGTGGAGAGTGGTGGTCAACATGTGGTGAGAAGCCCCGTCGTGGCTTCAAGCCTCATCAGTTCAGAGATAGCGTCGCAACAGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

773

Amino Acids

82.49

Weight (kDa)

6.07

Isoelectric Point (pI)

35.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 118 6.6e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 144 - 578 2.4e-43 Subtilase family
fn3_6 PF17766 654 - 758 7.5e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1248
AarI CACCTGC 1 cut(s) 1177
AasI GACNNNNNNGTC 1 cut(s) 1624
Acc16I TGCGCA 1 cut(s) 1671
Acc36I ACCTGC 1 cut(s) 1177
Acc65I GGTACC 1 cut(s) 674
AccB1I GGYRCC 2 cut(s) 545, 674
AccI GTMKAC 3 cut(s) 689, 1103, 2072
AccIII TCCGGA 2 cut(s) 404, 2037
AciI CCGC 6 cut(s) 696, 753, 971, 1332, 1563, 1704
AclWI GGATC 4 cut(s) 396, 905, 918, 1695
AcoI YGGCCR 4 cut(s) 413, 470, 777, 2049
AcsI RAATTY 5 cut(s) 646, 1055, 1120, 2008, 2033
AcuI CTGAAG 3 cut(s) 318, 684, 1523
AdeI CACNNNGTG 1 cut(s) 1451
AfaI GTAC 6 cut(s) 155, 676, 785, 1042, 1254, 2019
AfiI CCNNNNNNNGG 7 cut(s) 411, 1141, 1355, 1822, 2044, 2102, 2272
AflIII ACRYGT 1 cut(s) 2251
AgeI ACCGGT 1 cut(s) 1739
AjiI CACGTC 1 cut(s) 966
AjnI CCWGG 4 cut(s) 739, 1212, 1522, 1536
AjuI GAANNNNNNNTTGG 2 cut(s) 1311, 1343
Alw21I GWGCWC 1 cut(s) 1888
Alw26I GTCTC 2 cut(s) 73, 287
Alw44I GTGCAC 1 cut(s) 1884
AlwI GGATC 4 cut(s) 396, 905, 918, 1695
Ama87I CYCGRG 1 cut(s) 1835
Aor13HI TCCGGA 2 cut(s) 404, 2037
AoxI GGCC 9 cut(s) 303, 388, 413, 470, 777, 1475, 1547, 1824, 2049
ApaLI GTGCAC 1 cut(s) 1884
ApeKI GCWGC 8 cut(s) 830, 1034, 1257, 1541, 1565, 1640, 1652, 1691
ApoI RAATTY 5 cut(s) 646, 1055, 1120, 2008, 2033
ArsI GACNNNNNNTTYG 2 cut(s) 1040, 1072
AsiGI ACCGGT 1 cut(s) 1739
Asp700I GAANNNNTTC 1 cut(s) 269
Asp718I GGTACC 1 cut(s) 674
AspA2I CCTAGG 1 cut(s) 764
AspLEI GCGC 5 cut(s) 772, 793, 1262, 1657, 1672
AspS9I GGNCC 6 cut(s) 207, 983, 1475, 1497, 1825, 2186
AsuC2I CCSGG 6 cut(s) 205, 1052, 1116, 1804, 1836, 1837
AsuHPI GGTGA 4 cut(s) 190, 1370, 2165, 2269
AsuII TTCGAA 2 cut(s) 422, 1059
AvaI CYCGRG 1 cut(s) 1835
AvaII GGWCC 4 cut(s) 207, 983, 1497, 2186
AvrII CCTAGG 1 cut(s) 764
BaeGI GKGCMC 2 cut(s) 1634, 1888
BaeI ACNNNNGTAYC 2 cut(s) 994, 1027
BalI TGGCCA 3 cut(s) 472, 779, 2051
BamHI GGATCC 1 cut(s) 910
BanI GGYRCC 2 cut(s) 545, 674
BanII GRGCYC 3 cut(s) 612, 1688, 1842
Bbv12I GWGCWC 1 cut(s) 1888
BbvI GCAGC 8 cut(s) 817, 1046, 1269, 1528, 1577, 1639, 1652, 1678
BccI CCATC 6 cut(s) 56, 491, 516, 884, 1802, 1925
BceAI ACGGC 3 cut(s) 445, 1462, 1553
BcgI CGANNNNNNTGC 2 cut(s) 798, 832
BciT130I CCWGG 4 cut(s) 741, 1214, 1524, 1538
BciVI GTATCC 3 cut(s) 652, 1022, 1757
BclI TGATCA 3 cut(s) 139, 441, 1365
BcnI CCSGG 6 cut(s) 205, 1052, 1116, 1804, 1836, 1837
BcoDI GTCTC 2 cut(s) 73, 287
BfaI CTAG 9 cut(s) 6, 320, 765, 1458, 1470, 1485, 1782, 1790, 1829
BfoI RGCGCY 1 cut(s) 794
BfuAI ACCTGC 1 cut(s) 1177
BfuI GTATCC 3 cut(s) 652, 1022, 1757
BglII AGATCT 1 cut(s) 1960
BlnI CCTAGG 1 cut(s) 764
BlpI GCTNAGC 1 cut(s) 2162
Bme18I GGWCC 4 cut(s) 207, 983, 1497, 2186
BmeT110I CYCGRG 1 cut(s) 1835
BmgBI CACGTC 1 cut(s) 966
BmgT120I GGNCC 6 cut(s) 207, 983, 1475, 1497, 1825, 2186
BmsI GCATC 4 cut(s) 111, 728, 1081, 1147
BpmI CTGGAG 2 cut(s) 382, 1506
Bpu1102I GCTNAGC 1 cut(s) 2162
Bpu14I TTCGAA 2 cut(s) 422, 1059
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 6 cut(s) 205, 1052, 1116, 1804, 1836, 1837
BsaAI YACGTR 2 cut(s) 157, 709
BsaWI WCCGGW 4 cut(s) 404, 1141, 1739, 2037
BsaXI ACNNNNNCTCC 2 cut(s) 849, 879
Bsc4I CCNNNNNNNGG 7 cut(s) 411, 1141, 1355, 1822, 2044, 2102, 2272
Bse118I RCCGGY 3 cut(s) 349, 415, 1739
Bse3DI GCAATG 2 cut(s) 612, 982
BseAI TCCGGA 2 cut(s) 404, 2037
BseBI CCWGG 4 cut(s) 741, 1214, 1524, 1538
BseGI GGATG 7 cut(s) 99, 442, 507, 805, 868, 1314, 1619
BseLI CCNNNNNNNGG 7 cut(s) 411, 1141, 1355, 1822, 2044, 2102, 2272
BseMI GCAATG 2 cut(s) 612, 982
BseMII CTCAG 5 cut(s) 1059, 1334, 2153, 2165, 2216
BseRI GAGGAG 5 cut(s) 202, 205, 208, 364, 2162
BseSI GKGCMC 2 cut(s) 1634, 1888
BseXI GCAGC 8 cut(s) 817, 1046, 1269, 1528, 1577, 1639, 1652, 1678
BshFI GGCC 9 cut(s) 305, 390, 415, 472, 779, 1477, 1549, 1826, 2051
BshNI GGYRCC 2 cut(s) 545, 674
BshTI ACCGGT 1 cut(s) 1739
BsiHKAI GWGCWC 1 cut(s) 1888
BsiHKCI CYCGRG 1 cut(s) 1835
BslFI GGGAC 4 cut(s) 220, 1465, 1630, 2079
BslI CCNNNNNNNGG 7 cut(s) 411, 1141, 1355, 1822, 2044, 2102, 2272
BsmAI GTCTC 2 cut(s) 73, 287
BsmFI GGGAC 4 cut(s) 220, 1465, 1630, 2079
BsmI GAATGC 1 cut(s) 1985
BsnI GGCC 9 cut(s) 305, 390, 415, 472, 779, 1477, 1549, 1826, 2051
BsoBI CYCGRG 1 cut(s) 1835
Bsp119I TTCGAA 2 cut(s) 422, 1059
Bsp1286I GDGCHC 5 cut(s) 612, 1634, 1688, 1842, 1888
Bsp13I TCCGGA 2 cut(s) 404, 2037
Bsp1407I TGTACA 1 cut(s) 783
Bsp143I GATC 9 cut(s) 139, 401, 441, 910, 1200, 1237, 1365, 1700, 1960
Bsp1720I GCTNAGC 1 cut(s) 2162
BspACI CCGC 6 cut(s) 696, 753, 971, 1332, 1563, 1704
BspANI GGCC 9 cut(s) 305, 390, 415, 472, 779, 1477, 1549, 1826, 2051
BspCNI CTCAG 5 cut(s) 1060, 1335, 2154, 2166, 2217
BspEI TCCGGA 2 cut(s) 404, 2037
BspHI TCATGA 2 cut(s) 261, 2029
BspMI ACCTGC 1 cut(s) 1177
BspPI GGATC 4 cut(s) 396, 905, 918, 1695
BspT104I TTCGAA 2 cut(s) 422, 1059
BspT107I GGYRCC 2 cut(s) 545, 674
BsrDI GCAATG 2 cut(s) 612, 982
BsrFI RCCGGY 3 cut(s) 349, 415, 1739
BsrGI TGTACA 1 cut(s) 783
BssAI RCCGGY 3 cut(s) 349, 415, 1739
BssMI GATC 9 cut(s) 139, 401, 441, 910, 1200, 1237, 1365, 1700, 1960
BssT1I CCWWGG 3 cut(s) 764, 939, 1016
Bst2UI CCWGG 4 cut(s) 741, 1214, 1524, 1538
Bst4CI ACNGT 5 cut(s) 1030, 1611, 1943, 2047, 2070
Bst6I CTCTTC 1 cut(s) 235
BstAPI GCANNNNNTGC 1 cut(s) 1268
BstAUI TGTACA 1 cut(s) 783
BstBAI YACGTR 2 cut(s) 157, 709
BstBI TTCGAA 2 cut(s) 422, 1059
BstC8I GCNNGC 5 cut(s) 751, 843, 973, 1405, 2086
BstDEI CTNAG 6 cut(s) 1068, 1343, 1957, 2162, 2174, 2225
BstDSI CCRYGG 3 cut(s) 1095, 1493, 1569
BstF5I GGATG 7 cut(s) 99, 442, 507, 805, 868, 1314, 1619
BstH2I RGCGCY 1 cut(s) 794
BstHHI GCGC 5 cut(s) 772, 793, 1262, 1657, 1672
BstKTI GATC 9 cut(s) 142, 404, 444, 913, 1203, 1240, 1368, 1703, 1963
BstMAI GTCTC 2 cut(s) 73, 287
BstMBI GATC 9 cut(s) 139, 401, 441, 910, 1200, 1237, 1365, 1700, 1960
BstMWI GCNNNNNNNGC 7 cut(s) 321, 767, 776, 1268, 1442, 1649, 1799
BstNI CCWGG 4 cut(s) 741, 1214, 1524, 1538
BstNSI RCATGY 3 cut(s) 776, 1811, 2255
BstSLI GKGCMC 2 cut(s) 1634, 1888
BstV1I GCAGC 8 cut(s) 817, 1046, 1269, 1528, 1577, 1639, 1652, 1678
BstX2I RGATCY 4 cut(s) 401, 910, 1700, 1960
BstXI CCANNNNNNTGG 3 cut(s) 522, 757, 1643
BstYI RGATCY 4 cut(s) 401, 910, 1700, 1960
BsuI GTATCC 3 cut(s) 652, 1022, 1757
BsuRI GGCC 9 cut(s) 305, 390, 415, 472, 779, 1477, 1549, 1826, 2051
BtgI CCRYGG 3 cut(s) 1095, 1493, 1569
BtrI CACGTC 1 cut(s) 966
BtsCI GGATG 7 cut(s) 99, 442, 507, 805, 868, 1314, 1619
BtsI GCAGTG 2 cut(s) 285, 2214
BtsIMutI CAGTG 4 cut(s) 280, 285, 342, 2214
BveI ACCTGC 1 cut(s) 1177
Cac8I GCNNGC 5 cut(s) 751, 843, 973, 1405, 2086
CciI TCATGA 2 cut(s) 261, 2029
CfoI GCGC 5 cut(s) 772, 793, 1262, 1657, 1672
Cfr10I RCCGGY 3 cut(s) 349, 415, 1739
Cfr13I GGNCC 6 cut(s) 207, 983, 1475, 1497, 1825, 2186
Cfr9I CCCGGG 1 cut(s) 1835
CseI GACGC 1 cut(s) 2292
Csp6I GTAC 6 cut(s) 154, 675, 784, 1041, 1253, 2018
CspAI ACCGGT 1 cut(s) 1739
CviQI GTAC 6 cut(s) 154, 675, 784, 1041, 1253, 2018
DdeI CTNAG 6 cut(s) 1068, 1343, 1957, 2162, 2174, 2225
DpnI GATC 9 cut(s) 141, 403, 443, 912, 1202, 1239, 1367, 1702, 1962
DpnII GATC 9 cut(s) 139, 401, 441, 910, 1200, 1237, 1365, 1700, 1960
DraIII CACNNNGTG 1 cut(s) 1451
DrdI GACNNNNNNGTC 1 cut(s) 1624
DseDI GACNNNNNNGTC 1 cut(s) 1624
EaeI YGGCCR 4 cut(s) 413, 470, 777, 2049
Eam1104I CTCTTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 235
EciI GGCGGA 1 cut(s) 1693
Eco130I CCWWGG 3 cut(s) 764, 939, 1016
Eco147I AGGCCT 1 cut(s) 305
Eco24I GRGCYC 3 cut(s) 612, 1688, 1842
Eco47I GGWCC 4 cut(s) 207, 983, 1497, 2186
Eco57I CTGAAG 3 cut(s) 318, 684, 1523
Eco88I CYCGRG 1 cut(s) 1835
EcoO109I RGGNCCY 3 cut(s) 207, 983, 1825
EcoRI GAATTC 3 cut(s) 646, 1055, 2033
EcoRII CCWGG 4 cut(s) 739, 1212, 1522, 1536
EcoT14I CCWWGG 3 cut(s) 764, 939, 1016
EcoT38I GRGCYC 3 cut(s) 612, 1688, 1842
ErhI CCWWGG 3 cut(s) 764, 939, 1016
FalI AAGNNNNNCTT 4 cut(s) 1571, 1603, 2150, 2182
FaqI GGGAC 4 cut(s) 220, 1465, 1630, 2079
FauI CCCGC 2 cut(s) 964, 1339
FbaI TGATCA 3 cut(s) 139, 441, 1365
FblI GTMKAC 3 cut(s) 689, 1103, 2072
FokI GGATG 7 cut(s) 86, 449, 514, 812, 875, 1301, 1606
FriOI GRGCYC 3 cut(s) 612, 1688, 1842
FspAI RTGCGCAY 1 cut(s) 1671
FspBI CTAG 9 cut(s) 6, 320, 765, 1458, 1470, 1485, 1782, 1790, 1829
FspI TGCGCA 1 cut(s) 1671
GlaI GCGC 5 cut(s) 771, 792, 1261, 1656, 1671
GsuI CTGGAG 2 cut(s) 382, 1506
HaeII RGCGCY 1 cut(s) 794
HaeIII GGCC 9 cut(s) 305, 390, 415, 472, 779, 1477, 1549, 1826, 2051
HgaI GACGC 1 cut(s) 2292
HhaI GCGC 5 cut(s) 772, 793, 1262, 1657, 1672
Hin6I GCGC 5 cut(s) 770, 791, 1260, 1655, 1670
HinP1I GCGC 5 cut(s) 770, 791, 1260, 1655, 1670
HincII GTYRAC 4 cut(s) 1813, 1873, 2073, 2249
HindII GTYRAC 4 cut(s) 1813, 1873, 2073, 2249
HindIII AAGCTT 2 cut(s) 484, 1405
HinfI GANTC 5 cut(s) 110, 280, 1345, 2003, 2118
HpaI GTTAAC 1 cut(s) 1873
HphI GGTGA 4 cut(s) 190, 1370, 2165, 2269
Hpy188III TCNNGA 7 cut(s) 262, 399, 405, 1381, 1470, 2030, 2038
Hpy99I CGWCG 4 cut(s) 820, 1208, 2273, 2308
HpyAV CCTTC 7 cut(s) 533, 659, 974, 1055, 1349, 1886, 1962
HpyCH4III ACNGT 5 cut(s) 1030, 1611, 1943, 2047, 2070
HpyCH4IV ACGT 6 cut(s) 156, 708, 965, 1869, 2016, 2079
HpyCH4V TGCA 6 cut(s) 570, 1003, 1034, 1544, 1640, 1886
HpyF10VI GCNNNNNNNGC 7 cut(s) 321, 767, 776, 1268, 1442, 1649, 1799
HpyF3I CTNAG 6 cut(s) 1068, 1343, 1957, 2162, 2174, 2225
HpySE526I ACGT 6 cut(s) 156, 708, 965, 1869, 2016, 2079
HspAI GCGC 5 cut(s) 770, 791, 1260, 1655, 1670
Kpn2I TCCGGA 2 cut(s) 404, 2037
KpnI GGTACC 1 cut(s) 678
Ksp22I TGATCA 3 cut(s) 139, 441, 1365
KspAI GTTAAC 1 cut(s) 1873
Kzo9I GATC 9 cut(s) 139, 401, 441, 910, 1200, 1237, 1365, 1700, 1960
LmnI GCTCC 7 cut(s) 407, 766, 806, 1525, 1683, 1799, 1845
Lsp1109I GCAGC 8 cut(s) 817, 1046, 1269, 1528, 1577, 1639, 1652, 1678
LweI GCATC 4 cut(s) 111, 728, 1081, 1147
MaeI CTAG 9 cut(s) 6, 320, 765, 1458, 1470, 1485, 1782, 1790, 1829
MaeII ACGT 6 cut(s) 156, 708, 965, 1869, 2016, 2079
MaeIII GTNAC 6 cut(s) 8, 731, 1163, 1373, 1453, 2089
MalI GATC 9 cut(s) 141, 403, 443, 912, 1202, 1239, 1367, 1702, 1962
MboI GATC 9 cut(s) 139, 401, 441, 910, 1200, 1237, 1365, 1700, 1960
MboII GAAGA 9 cut(s) 222, 324, 506, 1119, 1207, 1210, 1273, 1981, 2140
MfeI CAATTG 2 cut(s) 63, 1044
MflI RGATCY 4 cut(s) 401, 910, 1700, 1960
MhlI GDGCHC 5 cut(s) 612, 1634, 1688, 1842, 1888
MlsI TGGCCA 3 cut(s) 472, 779, 2051
MluNI TGGCCA 3 cut(s) 472, 779, 2051
MlyI GAGTC 4 cut(s) 289, 1354, 1997, 2127
MmeI TCCRAC 3 cut(s) 798, 2095, 2115
Mox20I TGGCCA 3 cut(s) 472, 779, 2051
MroI TCCGGA 2 cut(s) 404, 2037
MroXI GAANNNNTTC 1 cut(s) 269
MscI TGGCCA 3 cut(s) 472, 779, 2051
MseI TTAA 7 cut(s) 308, 489, 600, 1181, 1602, 1872, 1965
MslI CAYNNNNRTG 3 cut(s) 104, 520, 641
Msp20I TGGCCA 3 cut(s) 472, 779, 2051
MspA1I CMGCKG 3 cut(s) 753, 1037, 1643
MunI CAATTG 2 cut(s) 63, 1044
Mva1269I GAATGC 1 cut(s) 1985
MvaI CCWGG 4 cut(s) 741, 1214, 1524, 1538
MwoI GCNNNNNNNGC 7 cut(s) 321, 767, 776, 1268, 1442, 1649, 1799
NciI CCSGG 6 cut(s) 205, 1052, 1116, 1804, 1836, 1837
NdeII GATC 9 cut(s) 139, 401, 441, 910, 1200, 1237, 1365, 1700, 1960
NmuCI GTSAC 1 cut(s) 2089
NsbI TGCGCA 1 cut(s) 1671
NspI RCATGY 3 cut(s) 776, 1811, 2255
NspV TTCGAA 2 cut(s) 422, 1059
PagI TCATGA 2 cut(s) 261, 2029
PaqCI CACCTGC 1 cut(s) 1177
PceI AGGCCT 1 cut(s) 305
PciI ACATGT 1 cut(s) 2251
PcsI WCGNNNNNNNCGW 1 cut(s) 2085
PctI GAATGC 1 cut(s) 1985
PdmI GAANNNNTTC 1 cut(s) 269
PfeI GAWTC 1 cut(s) 110
PfoI TCCNGGA 1 cut(s) 1114
PinAI ACCGGT 1 cut(s) 1739
PleI GAGTC 4 cut(s) 288, 1353, 1997, 2126
PpsI GAGTC 4 cut(s) 288, 1353, 1997, 2126
Ppu21I YACGTR 2 cut(s) 157, 709
PpuMI RGGWCCY 2 cut(s) 207, 983
PscI ACATGT 1 cut(s) 2251
PsiI TTATAA 1 cut(s) 1248
Psp5II RGGWCCY 2 cut(s) 207, 983
Psp6I CCWGG 4 cut(s) 739, 1212, 1522, 1536
PspGI CCWGG 4 cut(s) 739, 1212, 1522, 1536
PspPI GGNCC 6 cut(s) 207, 983, 1475, 1497, 1825, 2186
PspPPI RGGWCCY 2 cut(s) 207, 983
PsuI RGATCY 4 cut(s) 401, 910, 1700, 1960
PvuII CAGCTG 2 cut(s) 1037, 1643
RsaI GTAC 6 cut(s) 155, 676, 785, 1042, 1254, 2019
RsaNI GTAC 6 cut(s) 154, 675, 784, 1041, 1253, 2018
RseI CAYNNNNRTG 3 cut(s) 104, 520, 641
SalI GTCGAC 1 cut(s) 2071
SaqAI TTAA 7 cut(s) 308, 489, 600, 1181, 1602, 1872, 1965
Sau3AI GATC 9 cut(s) 139, 401, 441, 910, 1200, 1237, 1365, 1700, 1960
Sau96I GGNCC 6 cut(s) 207, 983, 1475, 1497, 1825, 2186
SchI GAGTC 4 cut(s) 289, 1354, 1997, 2127
SduI GDGCHC 5 cut(s) 612, 1634, 1688, 1842, 1888
SfaNI GCATC 4 cut(s) 111, 728, 1081, 1147
SfuI TTCGAA 2 cut(s) 422, 1059
SinI GGWCC 4 cut(s) 207, 983, 1497, 2186
SmaI CCCGGG 1 cut(s) 1837
SmiMI CAYNNNNRTG 3 cut(s) 104, 520, 641
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
SseBI AGGCCT 1 cut(s) 305
SsiI CCGC 6 cut(s) 696, 753, 971, 1332, 1563, 1704
SspI AATATT 1 cut(s) 1858
SspMI CTAG 9 cut(s) 6, 320, 765, 1458, 1470, 1485, 1782, 1790, 1829
StuI AGGCCT 1 cut(s) 305
StyI CCWWGG 3 cut(s) 764, 939, 1016
TaaI ACNGT 5 cut(s) 1030, 1611, 1943, 2047, 2070
TaiI ACGT 6 cut(s) 159, 711, 968, 1872, 2019, 2082
TaqI TCGA 4 cut(s) 422, 1059, 1934, 2072
TatI WGTACW 1 cut(s) 783
TauI GCSGC 2 cut(s) 698, 756
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 7 cut(s) 308, 489, 600, 1181, 1602, 1872, 1965
Tru9I TTAA 7 cut(s) 308, 489, 600, 1181, 1602, 1872, 1965
TscAI CASTG 4 cut(s) 280, 292, 342, 2214
TseFI GTSAC 1 cut(s) 2089
TseI GCWGC 8 cut(s) 830, 1034, 1257, 1541, 1565, 1640, 1652, 1691
Tsp45I GTSAC 1 cut(s) 2089
TspGWI ACGGA 1 cut(s) 870
TspMI CCCGGG 1 cut(s) 1835
TspRI CASTG 4 cut(s) 280, 292, 342, 2214
VneI GTGCAC 1 cut(s) 1884
VpaK11BI GGWCC 4 cut(s) 207, 983, 1497, 2186
XapI RAATTY 5 cut(s) 646, 1055, 1120, 2008, 2033
XbaI TCTAGA 1 cut(s) 1469
XceI RCATGY 3 cut(s) 776, 1811, 2255
XmaI CCCGGG 1 cut(s) 1835
XmaJI CCTAGG 1 cut(s) 764
XmiI GTMKAC 3 cut(s) 689, 1103, 2072
XmnI GAANNNNTTC 1 cut(s) 269
XspI CTAG 9 cut(s) 6, 320, 765, 1458, 1470, 1485, 1782, 1790, 1829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.