pycom04g21060

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001268
Physical Location & Seq
Forward (+)
22410145 .. 22412439
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g21060.1

Sequence Viewer

Length: 2295 bp
ATGGGGCATAACAATGGGGTTCCTCTTCCCTTCATTTTCTCTCTTGTTGCATTGTTTCTGTTGGCTCTGCATGTCAATGTCTCTTTCGCAGATAGGTCGACGTATATTGTCCACATGGACAAGTCCCTCATGCCCAAGTCCTACTCTAGCCATGACCATTGGTATTCCTCCATTGTTGATTCATTCAAATCCGAAAACCCTACCTCATTCGATGGCAACAAAATCTTGCCTTCCATTCTGTACACCTATGACACTGCCTTTCATGGGTTCAGTGCAGTTCTATCTGCTGATGAATTAGTGACTCTAAAAAAGTCCCCGGGTTTCGTCTCAGCTTACGCTGACAAGTCCATTACGCTCGACACCACACACACCACTCAGTTTCTCAACCTCAATCCTTTCGCCGGGCTGTGGCCTGCTTCGGATTACGGAGACGACATCATCATTGGTGTCATTGATACCGGTCTCTGGCCGGAGAGTGAAAGCTTCAGAGATGAAGGTATGACCAAAAGTCATCCAGCTAGGTGGAAAGGAAAATGCGAGGTTGGACAAGAGTTCAATGCCTCTCTTTGTAACTATAAATTGATTGGAGCTCGATACTTTAATAAGGGTGTCATGGCTGCAAACCCCGGTGTCACACTCAGCATGAACTCGGCTAGAGACAGTGCAGGGCATGGGACGCATACATCTTCCACAGCTGCGGGGAACTACGTGGACGGGGCATCTTATTTTGGTTACGCTAAAGGAACAGCCAGAGGCGTAGCACCGCGTGCCAGAGTTGCCATGTACAAGGTTAGCTGGGATGAGGGGCGTTATGCCTCTGATGTTCTAGCTGGTATGGACCAAGCTATTGCTGATGGTGTGGATGTTATTTCAATTTCCTCTGGCTTTGACGACACACCATTGTACGAGGATCCCTTAGCGATTGCTTCATTTGCAGCCATGGAGAAGGGTGTGGTGGTTTCAGCTTCAGCCGGAAATGAAGGCCCAGAGCTTGGGAAATTGCACAATGGCATCCCTTGGGTCTTGACCGTTGCAGCCGGCACAATAGACCGCTCATTTGGAGGAGCATTAACCTTTGGTAATGGTTTAACCATTACTGGATTTACCTTGTTCCCAGCAAACGCCTTAGTAAAAAACTCGCCACTGGTTTACAACAAGACCTTCTCAGCATGCAACTCAACAGCAGCGCTAGCAACTGCACCAGATGCGATCATCATATGTGATGACACGGAGCCTATTTATTATCAAATATCTCACATCATTCAGTCGGGGCTTCTTGGAGCTATCTTTATTACCAGTAATCCTGAGATACGCGGATTAGGTTACGTTGCAACTCCTAGCGTTGTGGTGAATACGAAGGATGGGCGGACTGTGATCGAGTACGCACTAAAAAGTGAAAACCCTACTGTCAGCATAAATTTCCAACAAACGTTGCTCAATACAAAGCCTGCACCCGCTGCTGCTTCTTACACTTCGAGAGGTCCTTCAAAAAGTTATCCGGGCATTTTGAAGCCGGACATAATGGCCCCGGGGTCATTAGTTTTAGCTGCTTGGGTTCCCAAGGTTGCAGCAGGTAAGATCGGGTTCAATGTGAACTTACCTAGCAACTACAATTTGATATCCGGGACATCCATGGCATGCCCTCATGCTTCAGGTGTAGCTGCTCTACTGAAAGGTGCGCACCCAGAATGGAGTGCAGCAGCAATTAGATCTGCTTTGATGACCACAGCTAACCCATTGGACAATACTGGCAATCCAATTCTTGACGACGGTGACAATTTCAACTTTGCTTCACCATTAGCTATGGGAGCAGGCCACATCGATCCTAATAGAGCACTTGATCCGGGCCTAATATACGATGCAACTCCACAAGAATATGTCAATCTCTTATGCTCCACGAACTTTACGCGTAACCAAATCTTATCCATCACTAGATCACATGCCTATGATTGTTCCAAACCATCTTCTGATCTCAACTATCCATCTTTCATTGCGTTGTCCAACAATCATCACAAAACAAAGACAAGGGTTCAAACATATCAGAGGACCGTAACAAATGTGGGAGATGGCGCGGCCAGGTACATGGCTTCGGTGATTGCTCCAAAGGGTTCTCAGGTAACAGTCTCCCCGGAGATACTGATCTTTGCATACAAGTATGAAAAGCAAAGTTTTACGGTCACTATAAATTTCAATGCGAAGAAGAAAGGGAACGCTTCTTCTGGCGCACTTGTTTGGATCGAACAAAATGGAAAATACACTGTCAGGAGCCCAATTGTAGTGTCACCTCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

765

Amino Acids

81.59

Weight (kDa)

6.53

Isoelectric Point (pI)

30.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 119 9.9e-16 Peptidase inhibitor I9
Peptidase_S8 PF00082 143 - 581 1.5e-44 Subtilase family
fn3_6 PF17766 657 - 760 2.9e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1680
Acc36I ACCTGC 1 cut(s) 1562
AccB7I CCANNNNNTGG 1 cut(s) 992
AccBSI CCGCTC 1 cut(s) 1053
AccI GTMKAC 1 cut(s) 98
AccII CGCG 4 cut(s) 766, 1314, 1909, 2072
AciI CCGC 7 cut(s) 698, 764, 1051, 1314, 1366, 1455, 2072
AclI AACGTT 1 cut(s) 1430
AclWI GGATC 5 cut(s) 905, 918, 1817, 1835, 2243
AcoI YGGCCR 2 cut(s) 467, 2073
AcsI RAATTY 2 cut(s) 1417, 2185
AcuI CTGAAG 3 cut(s) 469, 951, 1635
AdeI CACNNNGTG 1 cut(s) 767
AfaI GTAC 5 cut(s) 242, 785, 905, 1382, 2081
AfeI AGCGCT 1 cut(s) 1188
AfiI CCNNNNNNNGG 5 cut(s) 264, 401, 408, 465, 992
AflIII ACRYGT 1 cut(s) 1907
AgeI ACCGGT 1 cut(s) 458
AgsI TTSAA 9 cut(s) 187, 556, 873, 1488, 1510, 1588, 1783, 2033, 2191
AhdI GACNNNNNGTC 1 cut(s) 507
AjnI CCWGG 1 cut(s) 2075
Alw21I GWGCWC 2 cut(s) 592, 1837
Alw26I GTCTC 6 cut(s) 85, 331, 423, 467, 651, 2128
AlwI GGATC 5 cut(s) 905, 918, 1817, 1835, 2243
Ama87I CYCGRG 2 cut(s) 316, 1528
Aor51HI AGCGCT 1 cut(s) 1188
AoxI GGCC 7 cut(s) 410, 467, 982, 1524, 1813, 1846, 2073
ApoI RAATTY 2 cut(s) 1417, 2185
ArsI GACNNNNNNTTYG 2 cut(s) 1040, 1072
AsiGI ACCGGT 1 cut(s) 458
AspLEI GCGC 4 cut(s) 1189, 1681, 2072, 2225
AspS9I GGNCC 6 cut(s) 838, 983, 1481, 1525, 1846, 2046
AsuHPI GGTGA 5 cut(s) 1360, 1784, 1785, 2104, 2274
AsuNHI GCTAGC 1 cut(s) 1189
AvaI CYCGRG 2 cut(s) 316, 1528
AvaII GGWCC 3 cut(s) 838, 1481, 2046
BamHI GGATCC 1 cut(s) 910
BanII GRGCYC 2 cut(s) 592, 2270
Bbv12I GWGCWC 2 cut(s) 592, 1837
BccI CCATC 7 cut(s) 206, 848, 1355, 1934, 1969, 1990, 2060
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BciT130I CCWGG 1 cut(s) 2077
BcoDI GTCTC 6 cut(s) 85, 331, 423, 467, 651, 2128
BfaI CTAG 8 cut(s) 147, 519, 654, 827, 1190, 1338, 1602, 1932
BfoI RGCGCY 1 cut(s) 1190
BfuAI ACCTGC 1 cut(s) 1562
BglII AGATCT 1 cut(s) 1709
Bme18I GGWCC 3 cut(s) 838, 1481, 2046
BmeRI GACNNNNNGTC 1 cut(s) 507
BmeT110I CYCGRG 2 cut(s) 316, 1528
BmgT120I GGNCC 6 cut(s) 838, 983, 1481, 1525, 1846, 2046
BmiI GGNNCC 6 cut(s) 21, 912, 1233, 1527, 1557, 2267
BmsI GCATC 4 cut(s) 728, 1020, 1195, 1849
BmtI GCTAGC 1 cut(s) 1193
Bpu10I CCTNAGC 1 cut(s) 916
Bsa29I ATCGAT 1 cut(s) 1821
BsaAI YACGTR 1 cut(s) 709
BsaBI GATNNNNATC 1 cut(s) 2138
BsaI GGTCTC 1 cut(s) 467
BsaWI WCCGGW 1 cut(s) 458
Bsc4I CCNNNNNNNGG 5 cut(s) 264, 401, 408, 465, 992
Bse118I RCCGGY 2 cut(s) 458, 1037
Bse1I ACTGG 4 cut(s) 1102, 1149, 1296, 1753
Bse3DI GCAATG 1 cut(s) 1989
Bse8I GATNNNNATC 1 cut(s) 2138
BseBI CCWGG 1 cut(s) 2077
BseCI ATCGAT 1 cut(s) 1821
BseGI GGATG 6 cut(s) 511, 805, 868, 1011, 1366, 1628
BseJI GATNNNNATC 1 cut(s) 2138
BseLI CCNNNNNNNGG 5 cut(s) 264, 401, 408, 465, 992
BseMI GCAATG 1 cut(s) 1989
BseMII CTCAG 6 cut(s) 342, 389, 652, 1179, 1296, 2126
BseNI ACTGG 4 cut(s) 1102, 1149, 1296, 1753
BseRI GAGGAG 1 cut(s) 1077
BseYI CCCAGC 2 cut(s) 795, 1114
BsgI GTGCAG 5 cut(s) 294, 684, 1182, 1434, 1716
Bsh1236I CGCG 4 cut(s) 766, 1314, 1909, 2072
BshFI GGCC 7 cut(s) 412, 469, 984, 1526, 1815, 1848, 2075
BshTI ACCGGT 1 cut(s) 458
BshVI ATCGAT 1 cut(s) 1821
BsiHKAI GWGCWC 2 cut(s) 592, 1837
BsiHKCI CYCGRG 2 cut(s) 316, 1528
BslFI GGGAC 4 cut(s) 109, 298, 688, 1639
BslI CCNNNNNNNGG 5 cut(s) 264, 401, 408, 465, 992
BsmAI GTCTC 6 cut(s) 85, 331, 423, 467, 651, 2128
BsmBI CGTCTC 2 cut(s) 331, 423
BsmFI GGGAC 4 cut(s) 109, 298, 688, 1639
BsnI GGCC 7 cut(s) 412, 469, 984, 1526, 1815, 1848, 2075
Bso31I GGTCTC 1 cut(s) 467
BsoBI CYCGRG 2 cut(s) 316, 1528
Bsp1286I GDGCHC 3 cut(s) 592, 1837, 2270
Bsp1407I TGTACA 2 cut(s) 240, 783
Bsp19I CCATGG 2 cut(s) 939, 1632
BspACI CCGC 7 cut(s) 698, 764, 1051, 1314, 1366, 1455, 2072
BspANI GGCC 7 cut(s) 412, 469, 984, 1526, 1815, 1848, 2075
BspCNI CTCAG 6 cut(s) 341, 388, 651, 1178, 1297, 2125
BspDI ATCGAT 1 cut(s) 1821
BspFNI CGCG 4 cut(s) 766, 1314, 1909, 2072
BspLI GGNNCC 6 cut(s) 21, 912, 1233, 1527, 1557, 2267
BspMI ACCTGC 1 cut(s) 1562
BspOI GCTAGC 1 cut(s) 1193
BspPI GGATC 5 cut(s) 905, 918, 1817, 1835, 2243
BspTNI GGTCTC 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 1053
BsrDI GCAATG 1 cut(s) 1989
BsrFI RCCGGY 2 cut(s) 458, 1037
BsrGI TGTACA 2 cut(s) 240, 783
BsrI ACTGG 4 cut(s) 1102, 1149, 1296, 1753
BssAI RCCGGY 2 cut(s) 458, 1037
BssT1I CCWWGG 4 cut(s) 939, 1016, 1560, 1632
Bst2UI CCWGG 1 cut(s) 2077
Bst4CI ACNGT 9 cut(s) 662, 1030, 1372, 1408, 1772, 2050, 2122, 2176, 2260
Bst6I CTCTTC 1 cut(s) 30
BstAPI GCANNNNNTGC 3 cut(s) 767, 1205, 1457
BstAUI TGTACA 2 cut(s) 240, 783
BstBAI YACGTR 1 cut(s) 709
BstC8I GCNNGC 8 cut(s) 414, 768, 1039, 1171, 1191, 1449, 1639, 1813
BstDEI CTNAG 8 cut(s) 328, 375, 638, 916, 1126, 1165, 1305, 2112
BstDSI CCRYGG 2 cut(s) 939, 1632
BstF5I GGATG 6 cut(s) 511, 805, 868, 1011, 1366, 1628
BstFNI CGCG 4 cut(s) 766, 1314, 1909, 2072
BstH2I RGCGCY 1 cut(s) 1190
BstHHI GCGC 4 cut(s) 1189, 1681, 2072, 2225
BstMAI GTCTC 6 cut(s) 85, 331, 423, 467, 651, 2128
BstMWI GCNNNNNNNGC 8 cut(s) 676, 767, 776, 932, 1190, 1205, 1457, 1808
BstNI CCWGG 1 cut(s) 2077
BstNSI RCATGY 4 cut(s) 74, 1173, 1641, 1943
BstUI CGCG 4 cut(s) 766, 1314, 1909, 2072
BstX2I RGATCY 2 cut(s) 910, 1709
BstXI CCANNNNNNTGG 2 cut(s) 522, 2083
BstYI RGATCY 2 cut(s) 910, 1709
Bsu15I ATCGAT 1 cut(s) 1821
BsuRI GGCC 7 cut(s) 412, 469, 984, 1526, 1815, 1848, 2075
BsuTUI ATCGAT 1 cut(s) 1821
BtgI CCRYGG 2 cut(s) 939, 1632
BtsCI GGATG 6 cut(s) 511, 805, 868, 1011, 1366, 1628
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 5 cut(s) 252, 277, 667, 1142, 2256
BveI ACCTGC 1 cut(s) 1562
Cac8I GCNNGC 8 cut(s) 414, 768, 1039, 1171, 1191, 1449, 1639, 1813
CfoI GCGC 4 cut(s) 1189, 1681, 2072, 2225
Cfr10I RCCGGY 2 cut(s) 458, 1037
Cfr13I GGNCC 6 cut(s) 838, 983, 1481, 1525, 1846, 2046
Cfr9I CCCGGG 2 cut(s) 316, 1528
ClaI ATCGAT 1 cut(s) 1821
CseI GACGC 1 cut(s) 685
Csp6I GTAC 5 cut(s) 241, 784, 904, 1381, 2080
CspAI ACCGGT 1 cut(s) 458
CviQI GTAC 5 cut(s) 241, 784, 904, 1381, 2080
DdeI CTNAG 8 cut(s) 328, 375, 638, 916, 1126, 1165, 1305, 2112
DraIII CACNNNGTG 1 cut(s) 767
DriI GACNNNNNGTC 1 cut(s) 507
EaeI YGGCCR 2 cut(s) 467, 2073
Eam1104I CTCTTC 1 cut(s) 30
Eam1105I GACNNNNNGTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 30
EciI GGCGGA 1 cut(s) 1381
Ecl136II GAGCTC 1 cut(s) 590
Eco130I CCWWGG 4 cut(s) 939, 1016, 1560, 1632
Eco24I GRGCYC 2 cut(s) 592, 2270
Eco31I GGTCTC 1 cut(s) 467
Eco32I GATATC 1 cut(s) 1620
Eco47I GGWCC 3 cut(s) 838, 1481, 2046
Eco47III AGCGCT 1 cut(s) 1188
Eco53kI GAGCTC 1 cut(s) 590
Eco57I CTGAAG 3 cut(s) 469, 951, 1635
Eco88I CYCGRG 2 cut(s) 316, 1528
EcoICRI GAGCTC 1 cut(s) 590
EcoO109I RGGNCCY 1 cut(s) 1481
EcoRII CCWGG 1 cut(s) 2075
EcoRV GATATC 1 cut(s) 1620
EcoT14I CCWWGG 4 cut(s) 939, 1016, 1560, 1632
EcoT38I GRGCYC 2 cut(s) 592, 2270
ErhI CCWWGG 4 cut(s) 939, 1016, 1560, 1632
Esp3I CGTCTC 2 cut(s) 331, 423
FaqI GGGAC 4 cut(s) 109, 298, 688, 1639
FauI CCCGC 2 cut(s) 691, 1462
FauNDI CATATG 1 cut(s) 1217
FblI GTMKAC 1 cut(s) 98
FokI GGATG 6 cut(s) 498, 812, 875, 998, 1373, 1615
FriOI GRGCYC 2 cut(s) 592, 2270
FspAI RTGCGCAY 1 cut(s) 1680
FspBI CTAG 8 cut(s) 147, 519, 654, 827, 1190, 1338, 1602, 1932
FspI TGCGCA 1 cut(s) 1680
GlaI GCGC 4 cut(s) 1188, 1680, 2071, 2224
GsaI CCCAGC 2 cut(s) 799, 1118
HaeII RGCGCY 1 cut(s) 1190
HaeIII GGCC 7 cut(s) 412, 469, 984, 1526, 1815, 1848, 2075
HgaI GACGC 1 cut(s) 685
HhaI GCGC 4 cut(s) 1189, 1681, 2072, 2225
Hin6I GCGC 4 cut(s) 1187, 1679, 2070, 2223
HinP1I GCGC 4 cut(s) 1187, 1679, 2070, 2223
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HindIII AAGCTT 1 cut(s) 481
HinfI GANTC 2 cut(s) 179, 301
HphI GGTGA 5 cut(s) 1360, 1784, 1785, 2104, 2274
Hpy166II GTNNAC 6 cut(s) 99, 112, 243, 712, 1150, 1594
Hpy188I TCNGA 6 cut(s) 193, 421, 488, 820, 1969, 2043
Hpy188III TCNNGA 5 cut(s) 1024, 1304, 1476, 1763, 2263
Hpy8I GTNNAC 6 cut(s) 99, 112, 243, 712, 1150, 1594
Hpy99I CGWCG 2 cut(s) 103, 1772
HpyAV CCTTC 8 cut(s) 40, 240, 488, 940, 974, 1171, 1351, 1494
HpyCH4III ACNGT 9 cut(s) 662, 1030, 1372, 1408, 1772, 2050, 2122, 2176, 2260
HpyCH4IV ACGT 4 cut(s) 101, 708, 1326, 1430
HpyF10VI GCNNNNNNNGC 8 cut(s) 676, 767, 776, 932, 1190, 1205, 1457, 1808
HpyF3I CTNAG 8 cut(s) 328, 375, 638, 916, 1126, 1165, 1305, 2112
HpySE526I ACGT 4 cut(s) 101, 708, 1326, 1430
HspAI GCGC 4 cut(s) 1187, 1679, 2070, 2223
KroI GCCGGC 1 cut(s) 1037
KroNI GCCGGC 1 cut(s) 1039
LmnI GCTCC 8 cut(s) 587, 1064, 1231, 1280, 1808, 1898, 2104, 2265
LweI GCATC 4 cut(s) 728, 1020, 1195, 1849
MaeI CTAG 8 cut(s) 147, 519, 654, 827, 1190, 1338, 1602, 1932
MaeII ACGT 4 cut(s) 101, 708, 1326, 1430
MbiI CCGCTC 1 cut(s) 1053
MboII GAAGA 6 cut(s) 17, 678, 1956, 2208, 2209, 2212
MfeI CAATTG 1 cut(s) 2271
MflI RGATCY 2 cut(s) 910, 1709
MhlI GDGCHC 3 cut(s) 592, 1837, 2270
MluI ACGCGT 1 cut(s) 1907
MlyI GAGTC 1 cut(s) 295
MmeI TCCRAC 3 cut(s) 523, 1447, 2025
MroNI GCCGGC 1 cut(s) 1037
MseI TTAA 3 cut(s) 600, 1070, 1088
MslI CAYNNNNRTG 3 cut(s) 12, 75, 1944
MspA1I CMGCKG 2 cut(s) 695, 1457
MunI CAATTG 1 cut(s) 2271
MvaI CCWGG 1 cut(s) 2077
MvnI CGCG 4 cut(s) 766, 1314, 1909, 2072
MwoI GCNNNNNNNGC 8 cut(s) 676, 767, 776, 932, 1190, 1205, 1457, 1808
NaeI GCCGGC 1 cut(s) 1039
NcoI CCATGG 2 cut(s) 939, 1632
NdeI CATATG 1 cut(s) 1217
NgoMIV GCCGGC 1 cut(s) 1037
NheI GCTAGC 1 cut(s) 1189
NlaIV GGNNCC 6 cut(s) 21, 912, 1233, 1527, 1557, 2267
NmeAIII GCCGAG 1 cut(s) 629
NmuCI GTSAC 5 cut(s) 298, 631, 1772, 2176, 2280
NsbI TGCGCA 1 cut(s) 1680
NspI RCATGY 4 cut(s) 74, 1173, 1641, 1943
PaeI GCATGC 2 cut(s) 1173, 1641
PdiI GCCGGC 1 cut(s) 1039
PfeI GAWTC 1 cut(s) 179
PflMI CCANNNNNTGG 1 cut(s) 992
PfoI TCCNGGA 1 cut(s) 1622
PinAI ACCGGT 1 cut(s) 458
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Ppu21I YACGTR 1 cut(s) 709
PpuMI RGGWCCY 1 cut(s) 1481
Psp124BI GAGCTC 1 cut(s) 592
Psp1406I AACGTT 1 cut(s) 1430
Psp5II RGGWCCY 1 cut(s) 1481
Psp6I CCWGG 1 cut(s) 2075
PspFI CCCAGC 2 cut(s) 795, 1114
PspGI CCWGG 1 cut(s) 2075
PspN4I GGNNCC 6 cut(s) 21, 912, 1233, 1527, 1557, 2267
PspPI GGNCC 6 cut(s) 838, 983, 1481, 1525, 1846, 2046
PspPPI RGGWCCY 1 cut(s) 1481
PsuI RGATCY 2 cut(s) 910, 1709
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 5 cut(s) 242, 785, 905, 1382, 2081
RsaNI GTAC 5 cut(s) 241, 784, 904, 1381, 2080
RseI CAYNNNNRTG 3 cut(s) 12, 75, 1944
SacI GAGCTC 1 cut(s) 592
SalI GTCGAC 1 cut(s) 97
SaqAI TTAA 3 cut(s) 600, 1070, 1088
Sau96I GGNCC 6 cut(s) 838, 983, 1481, 1525, 1846, 2046
SchI GAGTC 1 cut(s) 295
SduI GDGCHC 3 cut(s) 592, 1837, 2270
SfaNI GCATC 4 cut(s) 728, 1020, 1195, 1849
SinI GGWCC 3 cut(s) 838, 1481, 2046
SmaI CCCGGG 2 cut(s) 318, 1530
SmiMI CAYNNNNRTG 3 cut(s) 12, 75, 1944
SphI GCATGC 2 cut(s) 1173, 1641
SsiI CCGC 7 cut(s) 698, 764, 1051, 1314, 1366, 1455, 2072
SspMI CTAG 8 cut(s) 147, 519, 654, 827, 1190, 1338, 1602, 1932
SstI GAGCTC 1 cut(s) 592
StyI CCWWGG 4 cut(s) 939, 1016, 1560, 1632
TaaI ACNGT 9 cut(s) 662, 1030, 1372, 1408, 1772, 2050, 2122, 2176, 2260
TaiI ACGT 4 cut(s) 104, 711, 1329, 1433
TaqI TCGA 8 cut(s) 98, 210, 357, 592, 1377, 1475, 1821, 2238
TatI WGTACW 2 cut(s) 240, 783
TauI GCSGC 1 cut(s) 2075
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 3 cut(s) 600, 1070, 1088
Tru9I TTAA 3 cut(s) 600, 1070, 1088
TscAI CASTG 5 cut(s) 259, 277, 667, 1149, 2263
TseFI GTSAC 5 cut(s) 298, 631, 1772, 2176, 2280
Tsp45I GTSAC 5 cut(s) 298, 631, 1772, 2176, 2280
TspGWI ACGGA 2 cut(s) 441, 1244
TspMI CCCGGG 2 cut(s) 316, 1528
TspRI CASTG 5 cut(s) 259, 277, 667, 1149, 2263
Van91I CCANNNNNTGG 1 cut(s) 992
VpaK11BI GGWCC 3 cut(s) 838, 1481, 2046
XapI RAATTY 2 cut(s) 1417, 2185
XceI RCATGY 4 cut(s) 74, 1173, 1641, 1943
XmaI CCCGGG 2 cut(s) 316, 1528
XmiI GTMKAC 1 cut(s) 98
XspI CTAG 8 cut(s) 147, 519, 654, 827, 1190, 1338, 1602, 1932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.