subtilisin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1251 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1627 |
| Acc16I | TGCGCA | 1 cut(s) | 1074 |
| Acc36I | ACCTGC | 1 cut(s) | 1031 |
| Acc65I | GGTACC | 1 cut(s) | 998 |
| AccB1I | GGYRCC | 3 cut(s) | 548, 998, 2095 |
| AccI | GTMKAC | 1 cut(s) | 692 |
| AccII | CGCG | 1 cut(s) | 1568 |
| AccIII | TCCGGA | 3 cut(s) | 407, 2040, 2128 |
| AciI | CCGC | 7 cut(s) | 177, 225, 699, 1335, 1568, 1655, 2210 |
| AclI | AACGTT | 1 cut(s) | 167 |
| AclWI | GGATC | 2 cut(s) | 908, 921 |
| AcoI | YGGCCR | 7 cut(s) | 416, 473, 696, 714, 780, 1397, 1569 |
| AcsI | RAATTY | 4 cut(s) | 649, 1058, 1123, 2036 |
| AcuI | CTGAAG | 1 cut(s) | 321 |
| AdeI | CACNNNGTG | 1 cut(s) | 1454 |
| AfaI | GTAC | 6 cut(s) | 158, 788, 1000, 1045, 1238, 2022 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 414, 995, 1101, 1358, 2065, 2105, 2275 |
| AflIII | ACRYGT | 1 cut(s) | 2254 |
| AhlI | ACTAGT | 1 cut(s) | 5 |
| AjiI | CACGTC | 1 cut(s) | 442 |
| AjnI | CCWGG | 4 cut(s) | 322, 742, 1215, 1525 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1314, 1346 |
| AleI | CACNNNNGTG | 1 cut(s) | 2150 |
| Alw21I | GWGCWC | 1 cut(s) | 1891 |
| Alw26I | GTCTC | 2 cut(s) | 76, 290 |
| Alw44I | GTGCAC | 1 cut(s) | 1887 |
| AlwI | GGATC | 2 cut(s) | 908, 921 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 749, 2164 |
| Ama87I | CYCGRG | 1 cut(s) | 1838 |
| Aor13HI | TCCGGA | 3 cut(s) | 407, 2040, 2128 |
| ApaLI | GTGCAC | 1 cut(s) | 1887 |
| ApeKI | GCWGC | 5 cut(s) | 833, 1037, 1260, 1544, 1694 |
| ApoI | RAATTY | 4 cut(s) | 649, 1058, 1123, 2036 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 272 |
| Asp718I | GGTACC | 1 cut(s) | 998 |
| AspA2I | CCTAGG | 1 cut(s) | 2059 |
| AspLEI | GCGC | 3 cut(s) | 775, 1075, 1660 |
| AspS9I | GGNCC | 9 cut(s) | 678, 986, 1104, 1437, 1478, 1500, 1522, 2069, 2189 |
| AsuC2I | CCSGG | 5 cut(s) | 718, 1119, 1498, 1839, 1840 |
| AsuHPI | GGTGA | 6 cut(s) | 137, 658, 1373, 1778, 2261, 2272 |
| AvaI | CYCGRG | 1 cut(s) | 1838 |
| AvaII | GGWCC | 5 cut(s) | 678, 986, 1104, 1500, 2189 |
| AvrII | CCTAGG | 1 cut(s) | 2059 |
| BaeGI | GKGCMC | 1 cut(s) | 1891 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 997, 1030 |
| BalI | TGGCCA | 3 cut(s) | 418, 475, 782 |
| BamHI | GGATCC | 1 cut(s) | 913 |
| BanI | GGYRCC | 3 cut(s) | 548, 998, 2095 |
| BanII | GRGCYC | 3 cut(s) | 615, 1691, 1845 |
| BbsI | GAAGAC | 1 cut(s) | 810 |
| Bbv12I | GWGCWC | 1 cut(s) | 1891 |
| BbvI | GCAGC | 5 cut(s) | 820, 1049, 1272, 1531, 1681 |
| BccI | CCATC | 7 cut(s) | 59, 494, 519, 887, 1805, 1928, 2080 |
| BceAI | ACGGC | 3 cut(s) | 448, 711, 1412 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 812, 846 |
| BciT130I | CCWGG | 4 cut(s) | 324, 744, 1217, 1527 |
| BciVI | GTATCC | 1 cut(s) | 1025 |
| BclI | TGATCA | 3 cut(s) | 142, 444, 1368 |
| BcnI | CCSGG | 5 cut(s) | 718, 1119, 1498, 1839, 1840 |
| BcoDI | GTCTC | 2 cut(s) | 76, 290 |
| BcuI | ACTAGT | 1 cut(s) | 5 |
| BfaI | CTAG | 8 cut(s) | 6, 198, 768, 1473, 1785, 1793, 1832, 2060 |
| BfoI | RGCGCY | 1 cut(s) | 1661 |
| BfuAI | ACCTGC | 1 cut(s) | 1031 |
| BfuI | GTATCC | 1 cut(s) | 1025 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2093 |
| BglII | AGATCT | 2 cut(s) | 1703, 1963 |
| BisI | GCNGC | 8 cut(s) | 699, 834, 1038, 1261, 1545, 1569, 1656, 1695 |
| BlnI | CCTAGG | 1 cut(s) | 2059 |
| BlpI | GCTNAGC | 1 cut(s) | 2165 |
| BlsI | GCNGC | 8 cut(s) | 700, 835, 1039, 1262, 1546, 1570, 1657, 1696 |
| Bme1390I | CCNGG | 9 cut(s) | 324, 718, 744, 1119, 1217, 1498, 1527, 1839, 1840 |
| Bme18I | GGWCC | 5 cut(s) | 678, 986, 1104, 1500, 2189 |
| BmeT110I | CYCGRG | 1 cut(s) | 1838 |
| BmgBI | CACGTC | 1 cut(s) | 442 |
| BmgT120I | GGNCC | 9 cut(s) | 678, 986, 1104, 1437, 1478, 1500, 1522, 2069, 2189 |
| BmrFI | CCNGG | 9 cut(s) | 324, 718, 744, 1119, 1217, 1498, 1527, 1839, 1840 |
| BmrI | ACTGGG | 1 cut(s) | 376 |
| BmsI | GCATC | 6 cut(s) | 114, 731, 1084, 1150, 1290, 1843 |
| BmuI | ACTGGG | 1 cut(s) | 376 |
| BpiI | GAAGAC | 1 cut(s) | 810 |
| BpmI | CTGGAG | 1 cut(s) | 306 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2165 |
| BpuEI | CTTGAG | 1 cut(s) | 323 |
| BpuMI | CCSGG | 5 cut(s) | 718, 1119, 1498, 1839, 1840 |
| BsaWI | WCCGGW | 3 cut(s) | 407, 2040, 2128 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 414, 995, 1101, 1358, 2065, 2105, 2275 |
| Bse1I | ACTGG | 6 cut(s) | 353, 382, 419, 753, 1145, 1777 |
| Bse3DI | GCAATG | 2 cut(s) | 42, 1983 |
| BseAI | TCCGGA | 3 cut(s) | 407, 2040, 2128 |
| BseBI | CCWGG | 4 cut(s) | 324, 744, 1217, 1527 |
| BseGI | GGATG | 5 cut(s) | 102, 808, 871, 1317, 1622 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 414, 995, 1101, 1358, 2065, 2105, 2275 |
| BseMI | GCAATG | 2 cut(s) | 42, 1983 |
| BseMII | CTCAG | 4 cut(s) | 1337, 2156, 2168, 2219 |
| BseNI | ACTGG | 6 cut(s) | 353, 382, 419, 753, 1145, 1777 |
| BseRI | GAGGAG | 4 cut(s) | 205, 208, 211, 553 |
| BseSI | GKGCMC | 1 cut(s) | 1891 |
| BseX3I | CGGCCG | 1 cut(s) | 696 |
| BseXI | GCAGC | 5 cut(s) | 820, 1049, 1272, 1531, 1681 |
| BseYI | CCCAGC | 1 cut(s) | 1805 |
| Bsh1236I | CGCG | 1 cut(s) | 1568 |
| Bsh1285I | CGRYCG | 1 cut(s) | 699 |
| BshNI | GGYRCC | 3 cut(s) | 548, 998, 2095 |
| BsiEI | CGRYCG | 1 cut(s) | 699 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1891 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1838 |
| BsiSI | CCGG | 8 cut(s) | 408, 717, 1119, 1275, 1498, 1839, 2041, 2129 |
| BslFI | GGGAC | 3 cut(s) | 674, 691, 1633 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 414, 995, 1101, 1358, 2065, 2105, 2275 |
| BsmAI | GTCTC | 2 cut(s) | 76, 290 |
| BsmFI | GGGAC | 3 cut(s) | 674, 691, 1633 |
| BsmI | GAATGC | 1 cut(s) | 1988 |
| BsoBI | CYCGRG | 1 cut(s) | 1838 |
| Bsp1286I | GDGCHC | 4 cut(s) | 615, 1691, 1845, 1891 |
| Bsp13I | TCCGGA | 3 cut(s) | 407, 2040, 2128 |
| Bsp1407I | TGTACA | 1 cut(s) | 786 |
| Bsp143I | GATC | 8 cut(s) | 142, 444, 913, 1051, 1203, 1368, 1703, 1963 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2165 |
| Bsp19I | CCATGG | 1 cut(s) | 1572 |
| BspACI | CCGC | 7 cut(s) | 177, 225, 699, 1335, 1568, 1655, 2210 |
| BspCNI | CTCAG | 4 cut(s) | 1338, 2157, 2169, 2220 |
| BspEI | TCCGGA | 3 cut(s) | 407, 2040, 2128 |
| BspFNI | CGCG | 1 cut(s) | 1568 |
| BspHI | TCATGA | 1 cut(s) | 2032 |
| BspMI | ACCTGC | 1 cut(s) | 1031 |
| BspPI | GGATC | 2 cut(s) | 908, 921 |
| BspT107I | GGYRCC | 3 cut(s) | 548, 998, 2095 |
| BsrDI | GCAATG | 2 cut(s) | 42, 1983 |
| BsrGI | TGTACA | 1 cut(s) | 786 |
| BsrI | ACTGG | 6 cut(s) | 353, 382, 419, 753, 1145, 1777 |
| BssMI | GATC | 8 cut(s) | 142, 444, 913, 1051, 1203, 1368, 1703, 1963 |
| BssT1I | CCWWGG | 4 cut(s) | 942, 1019, 1572, 2059 |
| Bst2UI | CCWGG | 4 cut(s) | 324, 744, 1217, 1527 |
| Bst4CI | ACNGT | 5 cut(s) | 860, 1033, 1946, 2050, 2100 |
| Bst6I | CTCTTC | 1 cut(s) | 238 |
| BstAUI | TGTACA | 1 cut(s) | 786 |
| BstC8I | GCNNGC | 3 cut(s) | 846, 1408, 2089 |
| BstDEI | CTNAG | 5 cut(s) | 1346, 1960, 2165, 2177, 2228 |
| BstDSI | CCRYGG | 1 cut(s) | 1572 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2063 |
| BstF5I | GGATG | 5 cut(s) | 102, 808, 871, 1317, 1622 |
| BstFNI | CGCG | 1 cut(s) | 1568 |
| BstH2I | RGCGCY | 1 cut(s) | 1661 |
| BstHHI | GCGC | 3 cut(s) | 775, 1075, 1660 |
| BstKTI | GATC | 8 cut(s) | 145, 447, 916, 1054, 1206, 1371, 1706, 1966 |
| BstMAI | GTCTC | 2 cut(s) | 76, 290 |
| BstMBI | GATC | 8 cut(s) | 142, 444, 913, 1051, 1203, 1368, 1703, 1963 |
| BstMCI | CGRYCG | 1 cut(s) | 699 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 324, 770, 779, 1445, 1652, 1802, 2093 |
| BstNI | CCWGG | 4 cut(s) | 324, 744, 1217, 1527 |
| BstNSI | RCATGY | 3 cut(s) | 779, 1814, 2258 |
| BstSCI | CCNGG | 9 cut(s) | 322, 716, 742, 1117, 1215, 1496, 1525, 1837, 1838 |
| BstSLI | GKGCMC | 1 cut(s) | 1891 |
| BstUI | CGCG | 1 cut(s) | 1568 |
| BstV1I | GCAGC | 5 cut(s) | 820, 1049, 1272, 1531, 1681 |
| BstV2I | GAAGAC | 1 cut(s) | 810 |
| BstX2I | RGATCY | 3 cut(s) | 913, 1703, 1963 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 389, 525, 760, 1646 |
| BstYI | RGATCY | 3 cut(s) | 913, 1703, 1963 |
| BstZI | CGGCCG | 1 cut(s) | 696 |
| BsuI | GTATCC | 1 cut(s) | 1025 |
| BtgI | CCRYGG | 1 cut(s) | 1572 |
| BtrI | CACGTC | 1 cut(s) | 442 |
| BtsCI | GGATG | 5 cut(s) | 102, 808, 871, 1317, 1622 |
| BtsI | GCAGTG | 2 cut(s) | 204, 288 |
| BtsIMutI | CAGTG | 8 cut(s) | 126, 204, 283, 288, 345, 663, 760, 2055 |
| BveI | ACCTGC | 1 cut(s) | 1031 |
| Cac8I | GCNNGC | 3 cut(s) | 846, 1408, 2089 |
| CaiI | CAGNNNCTG | 2 cut(s) | 749, 2164 |
| CciI | TCATGA | 1 cut(s) | 2032 |
| CfoI | GCGC | 3 cut(s) | 775, 1075, 1660 |
| Cfr13I | GGNCC | 9 cut(s) | 678, 986, 1104, 1437, 1478, 1500, 1522, 2069, 2189 |
| Cfr9I | CCCGGG | 1 cut(s) | 1838 |
| CseI | GACGC | 1 cut(s) | 1778 |
| Csp6I | GTAC | 6 cut(s) | 157, 787, 999, 1044, 1237, 2021 |
| CviQI | GTAC | 6 cut(s) | 157, 787, 999, 1044, 1237, 2021 |
| DdeI | CTNAG | 5 cut(s) | 1346, 1960, 2165, 2177, 2228 |
| DpnI | GATC | 8 cut(s) | 144, 446, 915, 1053, 1205, 1370, 1705, 1965 |
| DpnII | GATC | 8 cut(s) | 142, 444, 913, 1051, 1203, 1368, 1703, 1963 |
| DraIII | CACNNNGTG | 1 cut(s) | 1454 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1627 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1627 |
| EaeI | YGGCCR | 7 cut(s) | 416, 473, 696, 714, 780, 1397, 1569 |
| EagI | CGGCCG | 1 cut(s) | 696 |
| Eam1104I | CTCTTC | 1 cut(s) | 238 |
| EarI | CTCTTC | 1 cut(s) | 238 |
| EciI | GGCGGA | 1 cut(s) | 214 |
| EclXI | CGGCCG | 1 cut(s) | 696 |
| Eco130I | CCWWGG | 4 cut(s) | 942, 1019, 1572, 2059 |
| Eco147I | AGGCCT | 1 cut(s) | 308 |
| Eco24I | GRGCYC | 3 cut(s) | 615, 1691, 1845 |
| Eco47I | GGWCC | 5 cut(s) | 678, 986, 1104, 1500, 2189 |
| Eco52I | CGGCCG | 1 cut(s) | 696 |
| Eco57I | CTGAAG | 1 cut(s) | 321 |
| Eco88I | CYCGRG | 1 cut(s) | 1838 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2063 |
| EcoO109I | RGGNCCY | 3 cut(s) | 678, 986, 1478 |
| EcoRI | GAATTC | 3 cut(s) | 649, 1058, 2036 |
| EcoRII | CCWGG | 4 cut(s) | 322, 742, 1215, 1525 |
| EcoT14I | CCWWGG | 4 cut(s) | 942, 1019, 1572, 2059 |
| EcoT38I | GRGCYC | 3 cut(s) | 615, 1691, 1845 |
| ErhI | CCWWGG | 4 cut(s) | 942, 1019, 1572, 2059 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1574, 1606, 2153, 2185 |
| FaqI | GGGAC | 3 cut(s) | 674, 691, 1633 |
| FauI | CCCGC | 1 cut(s) | 1342 |
| FbaI | TGATCA | 3 cut(s) | 142, 444, 1368 |
| FblI | GTMKAC | 1 cut(s) | 692 |
| Fnu4HI | GCNGC | 8 cut(s) | 699, 834, 1038, 1261, 1545, 1569, 1656, 1695 |
| FokI | GGATG | 5 cut(s) | 89, 815, 878, 1304, 1609 |
| FriOI | GRGCYC | 3 cut(s) | 615, 1691, 1845 |
| Fsp4HI | GCNGC | 8 cut(s) | 699, 834, 1038, 1261, 1545, 1569, 1656, 1695 |
| FspBI | CTAG | 8 cut(s) | 6, 198, 768, 1473, 1785, 1793, 1832, 2060 |
| FspI | TGCGCA | 1 cut(s) | 1074 |
| GlaI | GCGC | 3 cut(s) | 774, 1074, 1659 |
| GluI | GCNGC | 8 cut(s) | 699, 834, 1038, 1261, 1545, 1569, 1656, 1695 |
| GsaI | CCCAGC | 1 cut(s) | 1809 |
| GsuI | CTGGAG | 1 cut(s) | 306 |
| HaeII | RGCGCY | 1 cut(s) | 1661 |
| HapII | CCGG | 8 cut(s) | 408, 717, 1119, 1275, 1498, 1839, 2041, 2129 |
| HgaI | GACGC | 1 cut(s) | 1778 |
| HhaI | GCGC | 3 cut(s) | 775, 1075, 1660 |
| Hin6I | GCGC | 3 cut(s) | 773, 1073, 1658 |
| HinP1I | GCGC | 3 cut(s) | 773, 1073, 1658 |
| HincII | GTYRAC | 2 cut(s) | 1816, 1876 |
| HindII | GTYRAC | 2 cut(s) | 1816, 1876 |
| HindIII | AAGCTT | 3 cut(s) | 487, 581, 1408 |
| HinfI | GANTC | 5 cut(s) | 113, 173, 625, 1348, 2029 |
| HpaI | GTTAAC | 1 cut(s) | 1876 |
| HpaII | CCGG | 8 cut(s) | 408, 717, 1119, 1275, 1498, 1839, 2041, 2129 |
| HphI | GGTGA | 6 cut(s) | 137, 658, 1373, 1778, 2261, 2272 |
| Hpy188III | TCNNGA | 6 cut(s) | 408, 1055, 1473, 2033, 2041, 2129 |
| Hpy99I | CGWCG | 2 cut(s) | 1211, 2276 |
| HpyAV | CCTTC | 6 cut(s) | 1352, 1491, 1889, 1965, 1986, 2240 |
| HpyCH4III | ACNGT | 5 cut(s) | 860, 1033, 1946, 2050, 2100 |
| HpyCH4IV | ACGT | 6 cut(s) | 167, 441, 1232, 1872, 2019, 2082 |
| HpyCH4V | TGCA | 8 cut(s) | 281, 573, 1006, 1037, 1547, 1673, 1889, 1988 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 324, 770, 779, 1445, 1652, 1802, 2093 |
| HpyF3I | CTNAG | 5 cut(s) | 1346, 1960, 2165, 2177, 2228 |
| HpySE526I | ACGT | 6 cut(s) | 167, 441, 1232, 1872, 2019, 2082 |
| HspAI | GCGC | 3 cut(s) | 773, 1073, 1658 |
| KflI | GGGWCCC | 1 cut(s) | 678 |
| Kpn2I | TCCGGA | 3 cut(s) | 407, 2040, 2128 |
| KpnI | GGTACC | 1 cut(s) | 1002 |
| Ksp22I | TGATCA | 3 cut(s) | 142, 444, 1368 |
| KspAI | GTTAAC | 1 cut(s) | 1876 |
| Kzo9I | GATC | 8 cut(s) | 142, 444, 913, 1051, 1203, 1368, 1703, 1963 |
| LmnI | GCTCC | 5 cut(s) | 410, 769, 809, 1686, 1802 |
| Lsp1109I | GCAGC | 5 cut(s) | 820, 1049, 1272, 1531, 1681 |
| LweI | GCATC | 6 cut(s) | 114, 731, 1084, 1150, 1290, 1843 |
| MaeI | CTAG | 8 cut(s) | 6, 198, 768, 1473, 1785, 1793, 1832, 2060 |
| MaeII | ACGT | 6 cut(s) | 167, 441, 1232, 1872, 2019, 2082 |
| MaeIII | GTNAC | 7 cut(s) | 8, 734, 1096, 1166, 1456, 1766, 2050 |
| MalI | GATC | 8 cut(s) | 144, 446, 915, 1053, 1205, 1370, 1705, 1965 |
| MboI | GATC | 8 cut(s) | 142, 444, 913, 1051, 1203, 1368, 1703, 1963 |
| MfeI | CAATTG | 2 cut(s) | 66, 1047 |
| MflI | RGATCY | 3 cut(s) | 913, 1703, 1963 |
| MhlI | GDGCHC | 4 cut(s) | 615, 1691, 1845, 1891 |
| MlsI | TGGCCA | 3 cut(s) | 418, 475, 782 |
| MluNI | TGGCCA | 3 cut(s) | 418, 475, 782 |
| MlyI | GAGTC | 3 cut(s) | 167, 1357, 2023 |
| MmeI | TCCRAC | 2 cut(s) | 2098, 2118 |
| Mox20I | TGGCCA | 3 cut(s) | 418, 475, 782 |
| MroI | TCCGGA | 3 cut(s) | 407, 2040, 2128 |
| MroXI | GAANNNNTTC | 1 cut(s) | 272 |
| MscI | TGGCCA | 3 cut(s) | 418, 475, 782 |
| MseI | TTAA | 6 cut(s) | 311, 492, 603, 1605, 1875, 1968 |
| MslI | CAYNNNNRTG | 6 cut(s) | 107, 523, 644, 906, 1083, 2150 |
| Msp20I | TGGCCA | 3 cut(s) | 418, 475, 782 |
| MspI | CCGG | 8 cut(s) | 408, 717, 1119, 1275, 1498, 1839, 2041, 2129 |
| MspR9I | CCNGG | 9 cut(s) | 324, 718, 744, 1119, 1217, 1498, 1527, 1839, 1840 |
| MunI | CAATTG | 2 cut(s) | 66, 1047 |
| Mva1269I | GAATGC | 1 cut(s) | 1988 |
| MvaI | CCWGG | 4 cut(s) | 324, 744, 1217, 1527 |
| MvnI | CGCG | 1 cut(s) | 1568 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 324, 770, 779, 1445, 1652, 1802, 2093 |
| NciI | CCSGG | 5 cut(s) | 718, 1119, 1498, 1839, 1840 |
| NcoI | CCATGG | 1 cut(s) | 1572 |
| NdeII | GATC | 8 cut(s) | 142, 444, 913, 1051, 1203, 1368, 1703, 1963 |
| NmuCI | GTSAC | 3 cut(s) | 1096, 1766, 2050 |
| NsbI | TGCGCA | 1 cut(s) | 1074 |
| NspI | RCATGY | 3 cut(s) | 779, 1814, 2258 |
| OliI | CACNNNNGTG | 1 cut(s) | 2150 |
| PagI | TCATGA | 1 cut(s) | 2032 |
| PasI | CCCWGGG | 1 cut(s) | 1526 |
| PceI | AGGCCT | 1 cut(s) | 308 |
| PciI | ACATGT | 1 cut(s) | 2254 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 438, 2088 |
| PctI | GAATGC | 1 cut(s) | 1988 |
| PdmI | GAANNNNTTC | 1 cut(s) | 272 |
| PfeI | GAWTC | 2 cut(s) | 113, 625 |
| PfoI | TCCNGGA | 1 cut(s) | 1117 |
| PkrI | GCNGC | 8 cut(s) | 700, 835, 1039, 1262, 1546, 1570, 1657, 1696 |
| PleI | GAGTC | 3 cut(s) | 167, 1356, 2023 |
| PpsI | GAGTC | 3 cut(s) | 167, 1356, 2023 |
| PpuMI | RGGWCCY | 2 cut(s) | 678, 986 |
| PscI | ACATGT | 1 cut(s) | 2254 |
| PsiI | TTATAA | 1 cut(s) | 1251 |
| Psp1406I | AACGTT | 1 cut(s) | 167 |
| Psp5II | RGGWCCY | 2 cut(s) | 678, 986 |
| Psp6I | CCWGG | 4 cut(s) | 322, 742, 1215, 1525 |
| PspFI | CCCAGC | 1 cut(s) | 1805 |
| PspGI | CCWGG | 4 cut(s) | 322, 742, 1215, 1525 |
| PspPI | GGNCC | 9 cut(s) | 678, 986, 1104, 1437, 1478, 1500, 1522, 2069, 2189 |
| PspPPI | RGGWCCY | 2 cut(s) | 678, 986 |
| PstNI | CAGNNNCTG | 2 cut(s) | 749, 2164 |
| PsuI | RGATCY | 3 cut(s) | 913, 1703, 1963 |
| RsaI | GTAC | 6 cut(s) | 158, 788, 1000, 1045, 1238, 2022 |
| RsaNI | GTAC | 6 cut(s) | 157, 787, 999, 1044, 1237, 2021 |
| RseI | CAYNNNNRTG | 6 cut(s) | 107, 523, 644, 906, 1083, 2150 |
| SaqAI | TTAA | 6 cut(s) | 311, 492, 603, 1605, 1875, 1968 |
| SatI | GCNGC | 8 cut(s) | 699, 834, 1038, 1261, 1545, 1569, 1656, 1695 |
| Sau3AI | GATC | 8 cut(s) | 142, 444, 913, 1051, 1203, 1368, 1703, 1963 |
| Sau96I | GGNCC | 9 cut(s) | 678, 986, 1104, 1437, 1478, 1500, 1522, 2069, 2189 |
| SchI | GAGTC | 3 cut(s) | 167, 1357, 2023 |
| ScrFI | CCNGG | 9 cut(s) | 324, 718, 744, 1119, 1217, 1498, 1527, 1839, 1840 |
| SduI | GDGCHC | 4 cut(s) | 615, 1691, 1845, 1891 |
| SfaNI | GCATC | 6 cut(s) | 114, 731, 1084, 1150, 1290, 1843 |
| SinI | GGWCC | 5 cut(s) | 678, 986, 1104, 1500, 2189 |
| SmaI | CCCGGG | 1 cut(s) | 1840 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 107, 523, 644, 906, 1083, 2150 |
| SmlI | CTYRAG | 1 cut(s) | 302 |
| SmoI | CTYRAG | 1 cut(s) | 302 |
| SpeI | ACTAGT | 1 cut(s) | 5 |
| SseBI | AGGCCT | 1 cut(s) | 308 |
| SsiI | CCGC | 7 cut(s) | 177, 225, 699, 1335, 1568, 1655, 2210 |
| SspMI | CTAG | 8 cut(s) | 6, 198, 768, 1473, 1785, 1793, 1832, 2060 |
| StuI | AGGCCT | 1 cut(s) | 308 |
| StyD4I | CCNGG | 9 cut(s) | 322, 716, 742, 1117, 1215, 1496, 1525, 1837, 1838 |
| StyI | CCWWGG | 4 cut(s) | 942, 1019, 1572, 2059 |
| TaaI | ACNGT | 5 cut(s) | 860, 1033, 1946, 2050, 2100 |
| TaiI | ACGT | 6 cut(s) | 170, 444, 1235, 1875, 2022, 2085 |
| TaqI | TCGA | 2 cut(s) | 1937, 2075 |
| TatI | WGTACW | 2 cut(s) | 786, 1236 |
| TauI | GCSGC | 3 cut(s) | 701, 1571, 1658 |
| TfiI | GAWTC | 2 cut(s) | 113, 625 |
| Tru1I | TTAA | 6 cut(s) | 311, 492, 603, 1605, 1875, 1968 |
| Tru9I | TTAA | 6 cut(s) | 311, 492, 603, 1605, 1875, 1968 |
| TscAI | CASTG | 8 cut(s) | 126, 211, 283, 295, 345, 670, 760, 2055 |
| TseFI | GTSAC | 3 cut(s) | 1096, 1766, 2050 |
| TseI | GCWGC | 5 cut(s) | 833, 1037, 1260, 1544, 1694 |
| Tsp45I | GTSAC | 3 cut(s) | 1096, 1766, 2050 |
| TspGWI | ACGGA | 2 cut(s) | 1084, 2162 |
| TspMI | CCCGGG | 1 cut(s) | 1838 |
| TspRI | CASTG | 8 cut(s) | 126, 211, 283, 295, 345, 670, 760, 2055 |
| VneI | GTGCAC | 1 cut(s) | 1887 |
| VpaK11BI | GGWCC | 5 cut(s) | 678, 986, 1104, 1500, 2189 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2063 |
| XapI | RAATTY | 4 cut(s) | 649, 1058, 1123, 2036 |
| XbaI | TCTAGA | 1 cut(s) | 1472 |
| XceI | RCATGY | 3 cut(s) | 779, 1814, 2258 |
| XmaI | CCCGGG | 1 cut(s) | 1838 |
| XmaJI | CCTAGG | 1 cut(s) | 2059 |
| XmiI | GTMKAC | 1 cut(s) | 692 |
| XmnI | GAANNNNTTC | 1 cut(s) | 272 |
| XspI | CTAG | 8 cut(s) | 6, 198, 768, 1473, 1785, 1793, 1832, 2060 |