Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001268

Physical Location & Seq

Genomic Coordinates Forward (+)

31554468 .. 31556762

Gene Structure 2295 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1236900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2295 bp

ATGCGGCATAACAATGGGGTTCCTCTTCCCTTCATTTTCTCTCTTGTTGCATTGTTTCTGTTGGCTCTTCATGTCAATGTGTCTTTCGCAGATAGGTCGACATATATTGTCCACATGGACAAGTCCCTCATGCCCAAGTCCTACACTAGCCATGACCATTGGTACTCCTCCATCGTCGATTCATTCAAATCCGAATACCCTACCTCATTCGATGGCAACAAAATCTTGCCTTCCATTCTGTACACCTATGACAATGCCTTTCATGGGTTCAGTGCAGTTCTATCTGCTGATGAATTAGTGACTCTAAAAAAGTCCCCGGGTTTCGTCTCAGCTTATGCTGACAAGTCCATTACGCTCGACACCACCCACACCACTCAATTCCTCAACCTCAATCCCTTCGCCGGGCTGTGGCCTGCTTCGGATTACGGAGACGACATCATCATTGGTGTCATTGATACCGGTCTCTGGCCGGAGAGTGAAAGCTTCAGAGATGAAGGTATGACCAAAAGTCTTCCAGCTAGGTGGAAAGGAAAATGTGAGGTTGGACAAGAGTTCAATGCCTCTCTTTGTAACTATAAATTGATTGGAGCTCGATACTTTAACAAGGGTGTCATGGCTGCAAACCCCGGTGTCACACTTAGCATGAACTCGGCTAGAGACAGTGCAGGGCATGGGACGCATACATCTTCCACAGCTGCAGGGAACTACGTAGACGGGGCATCTTATTTTGGTTACGCTAAAGGCACAGCCAGAGGCGTAGCACCGTGTGCCAGAGTTGCCATGTACAAGGTTATCTGGGATGAGGGGCGCTATGCCTCTGATGTTCTAGCTGGTATGGACCAAGCTATTGCTGATGGTGTTGATGTTATTTCAATTTCCTCTGGCTTTGACGACACACCATTGTACGAGGATCCTGTAGCGATTGCTTCATTTGCAGCCATGGAGAAGGGTGTGGTGGTTTCAGCTTCGGCCGGAAATGAAGGCCCCGAGCTTGGGAAATTGCACAATGGCATCCCTTGGGTCTTGACCGTTGCAGCCGGCACAATAGACCGCTCATTTGGAGGAGCATTAACCTTTGGTAATGGTTTAACCATTACTGGATTTACCTTGTTTCCAGCAAACTCCTTAGTCGAAAACTCGCAACTGGTTTACAACAAGACATTCTCAGCATGCAACTCAACCGCAGCGCTAGAAACTGCCCCAGATGCGATCATCATATGCGATGACACTGTGCCTATTCGTAATCAAATATCTCACATCATCCAATCGGGGCTTCTTGGAGCTATCTTTATTACCAACAATCCTGAGATACGCGAATTAGGTCACGTTGCAACTCCTAGCGTTGTGGTGAATACGAAGGATGGGCGGACTGTGATCGAGTACGCACTAAAAAGTGAAAACCCTACTGTCAGCATAAATTTCCAACAAACGTTGCTCAATACAAAGCCTGCCCCCGCTGCTGCTTTTTACACTTCGAGAGGTCCTTCAAAAAGTTATCCGGGCATTTTGAAGCCGGACATAATGGCCCCGGGGTCATTAGTTTTAGCTGCTTGGGTTCCTAAGGTTGCAGCGGGTAAAATCGGGTTCAATGTGAACTTACCTAGCAACTACAATTTGATATCCGGGACATCCATGGCATGCCCTCATGCTTCAGGTGTAGCTGCTCTACTGAAAGGTGCGCATCCAGAATGGAGTGCAGCAGCAATTAGATCTGCTTTGATGACCACAGCTAACCCATTGGACAATACCGGCAATCCAATTCTTGACGACGGTGACAATTTCAAATTTGCTTCACCATTAGCTATGGGAGCAGGTCACATCGATCCTAATAGAGCACTTGATCCGGGCCTAATATACGATGCAACTCCACAAGAATATGTCAATCTCTTATGCTCCACCAACTTTACGCGTAACCAAATCTTATCCATCACTAGATCACACGCCTATGATTGTTCCAAACCATCTTCTGATCTCAACTATCCATCTTTCATCGCGTTGTACAACAATCATCACAAAACAAAGAAAAGGGTTCAAACATTTCAGAGGACCGTAACGAATGTGGGAGATGCCGCGGCTAGGTACAGGGCTTCGGTAACTGCTCCGAAGGGTTCTCAGGTAACAGTCTCCCCAGAGATACTGATCTTTGCATACAAGTATGAGAAGCAAAGTTTTACGGTCACTTTGAATTACAAAGCGAAGAAGAAAGGGAAGGCTTCTTCTGGTGCACTTGTTTGGATCGAACAAAATGGAAAATACACTGTCAGGAGCCCGATTGTAGTGTCACCACTTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

765

Amino Acids

82.03

Weight (kDa)

7.01

Isoelectric Point (pI)

30.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	34 - 119	2.6e-15	Peptidase inhibitor I9
Peptidase_S8	PF00082	143 - 581	2.2e-43	Subtilase family
fn3_6	PF17766	657 - 760	4.2e-29	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001268)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67090
fragaria_vesca	FvH4_1g16180 FvH4_2g34170 FvH4_2g34170 FvH4_2g34230 FvH4_6g01060 FvH4_6g01061
malus_domestica	MD04G1236800.v1.1 MD04G1236900.v1.1 MD08G1098100.v1.1 MD15G1080600.v1.1 MD15G1285300.v1.1
prunus_persica	Prupe.1G433300_v2.0.a1 Prupe.6G168400_v2.0.a1 Prupe.6G356800_v2.0.a1 Prupe.7G131900_v2.0.a1
pyrus_communis	pycom04g21060 pycom04g21070 pycom08g07990 pycom12g23460 pycom15g07490 pycom15g24800
rosa_chinensis	RchiOBHm_Chr2g0105861 RchiOBHm_Chr2g0105881 RchiOBHm_Chr3g0448571 RchiOBHm_Chr6g0308191
rosa_laevigata	RLG00000010660 RLG00000017466 RLG00000017467 RLG00000025886 RLG00000025888 RLG00000025889 RLG00000025890
rosa_multiflora	Rmu_sc0003116.1_g000043 Rmu_sc0004367.1_g000007 Rmu_sc0004367.1_g000008 Rmu_sc0005706.1_g000016 Rmu_sc0010389.1_g000023
rosa_roxburghii	Rroxscaffold_2G00137780 Rroxscaffold_6G00426820 Rroxscaffold_6G00426860 Rroxscaffold_7G00160370 Rroxscaffold_7G00160460
rosa_rugosa	Rorug02G0131300 Rorug02G0131500 Rorug02G0613500 Rorug02G0613600 Rorug06G0364600
rosa_samantha	Rh2CG186700 Rh3DG013500 Rh6DG475800
rosa_wichuraiana	Rw2G014280 Rw2G014290 Rw3G000970 Rw6G041320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1680
Acc36I	ACCTGC	1 cut(s)	1802
AccBSI	CCGCTC	1 cut(s)	1053
AccI	GTMKAC	2 cut(s)	98, 711
AccII	CGCG	4 cut(s)	1314, 1909, 1994, 2072
AciI	CCGC	8 cut(s)	4, 1051, 1182, 1366, 1455, 1571, 2070, 2072
AclI	AACGTT	1 cut(s)	1430
AclWI	GGATC	5 cut(s)	905, 918, 1817, 1835, 2243
AcoI	YGGCCR	2 cut(s)	467, 969
AcsI	RAATTY	2 cut(s)	1417, 1784
AcuI	CTGAAG	2 cut(s)	469, 1635
AdeI	CACNNNGTG	1 cut(s)	767
AfaI	GTAC	7 cut(s)	164, 242, 785, 905, 1382, 2000, 2081
AfeI	AGCGCT	1 cut(s)	1188
AfiI	CCNNNNNNNGG	8 cut(s)	264, 401, 402, 408, 465, 992, 993, 2076
AflIII	ACRYGT	1 cut(s)	1907
AgeI	ACCGGT	1 cut(s)	458
AgsI	TTSAA	9 cut(s)	187, 556, 873, 1488, 1510, 1588, 1783, 2033, 2185
AhdI	GACNNNNNGTC	1 cut(s)	507
AjuI	GAANNNNNNNTTGG	2 cut(s)	1257, 1289
Alw21I	GWGCWC	3 cut(s)	592, 1837, 2227
Alw26I	GTCTC	5 cut(s)	331, 423, 467, 651, 2128
Alw44I	GTGCAC	1 cut(s)	2223
AlwI	GGATC	5 cut(s)	905, 918, 1817, 1835, 2243
Ama87I	CYCGRG	3 cut(s)	316, 986, 1528
Aor51HI	AGCGCT	1 cut(s)	1188
AoxI	GGCC	6 cut(s)	410, 467, 969, 982, 1524, 1846
ApaLI	GTGCAC	1 cut(s)	2223
ApoI	RAATTY	2 cut(s)	1417, 1784
ArsI	GACNNNNNNTTYG	2 cut(s)	1040, 1072
AsiGI	ACCGGT	1 cut(s)	458
AspLEI	GCGC	3 cut(s)	810, 1189, 1681
AspS9I	GGNCC	6 cut(s)	838, 983, 1481, 1525, 1846, 2046
AsuC2I	CCSGG	9 cut(s)	317, 318, 403, 627, 1500, 1529, 1530, 1624, 1845
AsuHPI	GGTGA	4 cut(s)	1360, 1784, 1785, 2274
AvaI	CYCGRG	3 cut(s)	316, 986, 1528
AvaII	GGWCC	3 cut(s)	838, 1481, 2046
AxyI	CCTNAGG	1 cut(s)	1560
BaeGI	GKGCMC	1 cut(s)	2227
BamHI	GGATCC	1 cut(s)	910
BanII	GRGCYC	2 cut(s)	592, 2270
BbsI	GAAGAC	1 cut(s)	503
Bbv12I	GWGCWC	3 cut(s)	592, 1837, 2227
BccI	CCATC	7 cut(s)	179, 206, 848, 1355, 1934, 1969, 1990
BcgI	CGANNNNNNTGC	2 cut(s)	78, 112
BcnI	CCSGG	9 cut(s)	317, 318, 403, 627, 1500, 1529, 1530, 1624, 1845
BcoDI	GTCTC	5 cut(s)	331, 423, 467, 651, 2128
BfaI	CTAG	9 cut(s)	147, 519, 654, 827, 1190, 1338, 1602, 1932, 2076
BfmI	CTRYAG	2 cut(s)	696, 915
BfoI	RGCGCY	2 cut(s)	811, 1190
BfuAI	ACCTGC	1 cut(s)	1802
BglII	AGATCT	1 cut(s)	1709
Bme1390I	CCNGG	9 cut(s)	317, 318, 403, 627, 1500, 1529, 1530, 1624, 1845
Bme18I	GGWCC	3 cut(s)	838, 1481, 2046
BmeRI	GACNNNNNGTC	1 cut(s)	507
BmeT110I	CYCGRG	3 cut(s)	316, 986, 1528
BmgT120I	GGNCC	6 cut(s)	838, 983, 1481, 1525, 1846, 2046
BmiI	GGNNCC	6 cut(s)	21, 912, 985, 1527, 1557, 2267
BmrFI	CCNGG	9 cut(s)	317, 318, 403, 627, 1500, 1529, 1530, 1624, 1845
BmsI	GCATC	6 cut(s)	728, 1020, 1195, 1690, 1849, 2056
BpiI	GAAGAC	1 cut(s)	503
BpuMI	CCSGG	9 cut(s)	317, 318, 403, 627, 1500, 1529, 1530, 1624, 1845
Bsa29I	ATCGAT	1 cut(s)	1821
BsaAI	YACGTR	1 cut(s)	709
BsaBI	GATNNNNATC	1 cut(s)	2138
BsaI	GGTCTC	1 cut(s)	467
BsaWI	WCCGGW	1 cut(s)	458
Bsc4I	CCNNNNNNNGG	8 cut(s)	264, 401, 402, 408, 465, 992, 993, 2076
Bse118I	RCCGGY	3 cut(s)	458, 1037, 1748
Bse1I	ACTGG	2 cut(s)	1102, 1149
Bse21I	CCTNAGG	1 cut(s)	1560
Bse8I	GATNNNNATC	1 cut(s)	2138
BseCI	ATCGAT	1 cut(s)	1821
BseGI	GGATG	6 cut(s)	805, 1011, 1260, 1366, 1628, 1681
BseJI	GATNNNNATC	1 cut(s)	2138
BseLI	CCNNNNNNNGG	8 cut(s)	264, 401, 402, 408, 465, 992, 993, 2076
BseMII	CTCAG	4 cut(s)	342, 1179, 1296, 2126
BseNI	ACTGG	2 cut(s)	1102, 1149
BseRI	GAGGAG	2 cut(s)	157, 1077
BseSI	GKGCMC	1 cut(s)	2227
BseX3I	CGGCCG	1 cut(s)	969
BsgI	GTGCAG	3 cut(s)	294, 684, 1716
Bsh1236I	CGCG	4 cut(s)	1314, 1909, 1994, 2072
Bsh1285I	CGRYCG	1 cut(s)	972
BshFI	GGCC	6 cut(s)	412, 469, 971, 984, 1526, 1848
BshTI	ACCGGT	1 cut(s)	458
BshVI	ATCGAT	1 cut(s)	1821
BsiEI	CGRYCG	1 cut(s)	972
BsiHKAI	GWGCWC	3 cut(s)	592, 1837, 2227
BsiHKCI	CYCGRG	3 cut(s)	316, 986, 1528
BslFI	GGGAC	4 cut(s)	109, 298, 688, 1639
BslI	CCNNNNNNNGG	8 cut(s)	264, 401, 402, 408, 465, 992, 993, 2076
BsmAI	GTCTC	5 cut(s)	331, 423, 467, 651, 2128
BsmBI	CGTCTC	2 cut(s)	331, 423
BsmFI	GGGAC	4 cut(s)	109, 298, 688, 1639
BsnI	GGCC	6 cut(s)	412, 469, 971, 984, 1526, 1848
Bso31I	GGTCTC	1 cut(s)	467
BsoBI	CYCGRG	3 cut(s)	316, 986, 1528
Bsp1286I	GDGCHC	4 cut(s)	592, 1837, 2227, 2270
Bsp1407I	TGTACA	3 cut(s)	240, 783, 1998
Bsp19I	CCATGG	2 cut(s)	939, 1632
BspACI	CCGC	8 cut(s)	4, 1051, 1182, 1366, 1455, 1571, 2070, 2072
BspANI	GGCC	6 cut(s)	412, 469, 971, 984, 1526, 1848
BspCNI	CTCAG	4 cut(s)	341, 1178, 1297, 2125
BspDI	ATCGAT	1 cut(s)	1821
BspFNI	CGCG	4 cut(s)	1314, 1909, 1994, 2072
BspLI	GGNNCC	6 cut(s)	21, 912, 985, 1527, 1557, 2267
BspMAI	CTGCAG	1 cut(s)	700
BspMI	ACCTGC	1 cut(s)	1802
BspPI	GGATC	5 cut(s)	905, 918, 1817, 1835, 2243
BspQI	GCTCTTC	1 cut(s)	72
BspTNI	GGTCTC	1 cut(s)	467
BsrBI	CCGCTC	1 cut(s)	1053
BsrFI	RCCGGY	3 cut(s)	458, 1037, 1748
BsrGI	TGTACA	3 cut(s)	240, 783, 1998
BsrI	ACTGG	2 cut(s)	1102, 1149
BssAI	RCCGGY	3 cut(s)	458, 1037, 1748
BssT1I	CCWWGG	3 cut(s)	939, 1016, 1632
Bst6I	CTCTTC	2 cut(s)	30, 72
BstAPI	GCANNNNNTGC	1 cut(s)	767
BstAUI	TGTACA	3 cut(s)	240, 783, 1998
BstBAI	YACGTR	1 cut(s)	709
BstC8I	GCNNGC	5 cut(s)	414, 1039, 1171, 1449, 1639
BstDEI	CTNAG	7 cut(s)	328, 638, 1126, 1165, 1305, 1560, 2112
BstDSI	CCRYGG	3 cut(s)	939, 1632, 2070
BstF5I	GGATG	6 cut(s)	805, 1011, 1260, 1366, 1628, 1681
BstFNI	CGCG	4 cut(s)	1314, 1909, 1994, 2072
BstH2I	RGCGCY	2 cut(s)	811, 1190
BstHHI	GCGC	3 cut(s)	810, 1189, 1681
BstMAI	GTCTC	5 cut(s)	331, 423, 467, 651, 2128
BstMCI	CGRYCG	1 cut(s)	972
BstMWI	GCNNNNNNNGC	7 cut(s)	676, 767, 776, 932, 1205, 1457, 1808
BstNSI	RCATGY	2 cut(s)	1173, 1641
BstSCI	CCNGG	9 cut(s)	315, 316, 401, 625, 1498, 1527, 1528, 1622, 1843
BstSFI	CTRYAG	2 cut(s)	696, 915
BstSLI	GKGCMC	1 cut(s)	2227
BstSNI	TACGTA	1 cut(s)	709
BstUI	CGCG	4 cut(s)	1314, 1909, 1994, 2072
BstV2I	GAAGAC	1 cut(s)	503
BstX2I	RGATCY	2 cut(s)	910, 1709
BstXI	CCANNNNNNTGG	1 cut(s)	522
BstYI	RGATCY	2 cut(s)	910, 1709
BstZI	CGGCCG	1 cut(s)	969
Bsu15I	ATCGAT	1 cut(s)	1821
Bsu36I	CCTNAGG	1 cut(s)	1560
BsuRI	GGCC	6 cut(s)	412, 469, 971, 984, 1526, 1848
BsuTUI	ATCGAT	1 cut(s)	1821
BtgI	CCRYGG	3 cut(s)	939, 1632, 2070
BtgZI	GCGATG	2 cut(s)	1236, 1975
BtsCI	GGATG	6 cut(s)	805, 1011, 1260, 1366, 1628, 1681
BtsIMutI	CAGTG	4 cut(s)	277, 667, 1227, 2256
BveI	ACCTGC	1 cut(s)	1802
Cac8I	GCNNGC	5 cut(s)	414, 1039, 1171, 1449, 1639
CfoI	GCGC	3 cut(s)	810, 1189, 1681
Cfr10I	RCCGGY	3 cut(s)	458, 1037, 1748
Cfr13I	GGNCC	6 cut(s)	838, 983, 1481, 1525, 1846, 2046
Cfr42I	CCGCGG	1 cut(s)	2073
Cfr9I	CCCGGG	2 cut(s)	316, 1528
ClaI	ATCGAT	1 cut(s)	1821
CseI	GACGC	1 cut(s)	685
Csp6I	GTAC	7 cut(s)	163, 241, 784, 904, 1381, 1999, 2080
CspAI	ACCGGT	1 cut(s)	458
CviQI	GTAC	7 cut(s)	163, 241, 784, 904, 1381, 1999, 2080
DdeI	CTNAG	7 cut(s)	328, 638, 1126, 1165, 1305, 1560, 2112
DraIII	CACNNNGTG	1 cut(s)	767
DriI	GACNNNNNGTC	1 cut(s)	507
EaeI	YGGCCR	2 cut(s)	467, 969
EagI	CGGCCG	1 cut(s)	969
Eam1104I	CTCTTC	2 cut(s)	30, 72
Eam1105I	GACNNNNNGTC	1 cut(s)	507
EarI	CTCTTC	2 cut(s)	30, 72
EciI	GGCGGA	1 cut(s)	1381
Ecl136II	GAGCTC	1 cut(s)	590
EclXI	CGGCCG	1 cut(s)	969
Eco105I	TACGTA	1 cut(s)	709
Eco130I	CCWWGG	3 cut(s)	939, 1016, 1632
Eco24I	GRGCYC	2 cut(s)	592, 2270
Eco31I	GGTCTC	1 cut(s)	467
Eco32I	GATATC	1 cut(s)	1620
Eco47I	GGWCC	3 cut(s)	838, 1481, 2046
Eco47III	AGCGCT	1 cut(s)	1188
Eco52I	CGGCCG	1 cut(s)	969
Eco53kI	GAGCTC	1 cut(s)	590
Eco57I	CTGAAG	2 cut(s)	469, 1635
Eco81I	CCTNAGG	1 cut(s)	1560
Eco88I	CYCGRG	3 cut(s)	316, 986, 1528
EcoICRI	GAGCTC	1 cut(s)	590
EcoO109I	RGGNCCY	2 cut(s)	983, 1481
EcoRV	GATATC	1 cut(s)	1620
EcoT14I	CCWWGG	3 cut(s)	939, 1016, 1632
EcoT38I	GRGCYC	2 cut(s)	592, 2270
ErhI	CCWWGG	3 cut(s)	939, 1016, 1632
Esp3I	CGTCTC	2 cut(s)	331, 423
FaqI	GGGAC	4 cut(s)	109, 298, 688, 1639
FauI	CCCGC	2 cut(s)	1462, 1564
FauNDI	CATATG	1 cut(s)	1217
FblI	GTMKAC	2 cut(s)	98, 711
FokI	GGATG	6 cut(s)	812, 998, 1247, 1373, 1615, 1668
FriOI	GRGCYC	2 cut(s)	592, 2270
FspAI	RTGCGCAY	1 cut(s)	1680
FspBI	CTAG	9 cut(s)	147, 519, 654, 827, 1190, 1338, 1602, 1932, 2076
FspI	TGCGCA	1 cut(s)	1680
GlaI	GCGC	3 cut(s)	809, 1188, 1680
HaeII	RGCGCY	2 cut(s)	811, 1190
HaeIII	GGCC	6 cut(s)	412, 469, 971, 984, 1526, 1848
HgaI	GACGC	1 cut(s)	685
HhaI	GCGC	3 cut(s)	810, 1189, 1681
Hin6I	GCGC	3 cut(s)	808, 1187, 1679
HinP1I	GCGC	3 cut(s)	808, 1187, 1679
HincII	GTYRAC	1 cut(s)	99
HindII	GTYRAC	1 cut(s)	99
HindIII	AAGCTT	1 cut(s)	481
HinfI	GANTC	2 cut(s)	179, 301
HphI	GGTGA	4 cut(s)	1360, 1784, 1785, 2274
Hpy166II	GTNNAC	7 cut(s)	99, 112, 243, 712, 1150, 1594, 2225
Hpy188I	TCNGA	7 cut(s)	193, 421, 488, 820, 1969, 2043, 2103
Hpy188III	TCNNGA	6 cut(s)	1024, 1304, 1476, 1685, 1763, 2263
Hpy8I	GTNNAC	7 cut(s)	99, 112, 243, 712, 1150, 1594, 2225
Hpy99I	CGWCG	2 cut(s)	179, 1772
HpyCH4IV	ACGT	3 cut(s)	708, 1326, 1430
HpyF10VI	GCNNNNNNNGC	7 cut(s)	676, 767, 776, 932, 1205, 1457, 1808
HpyF3I	CTNAG	7 cut(s)	328, 638, 1126, 1165, 1305, 1560, 2112
HpySE526I	ACGT	3 cut(s)	708, 1326, 1430
HspAI	GCGC	3 cut(s)	808, 1187, 1679
KroI	GCCGGC	1 cut(s)	1037
KroNI	GCCGGC	1 cut(s)	1039
KspI	CCGCGG	1 cut(s)	2073
LguI	GCTCTTC	1 cut(s)	72
LmnI	GCTCC	7 cut(s)	587, 1064, 1280, 1808, 1898, 2104, 2265
LweI	GCATC	6 cut(s)	728, 1020, 1195, 1690, 1849, 2056
MaeI	CTAG	9 cut(s)	147, 519, 654, 827, 1190, 1338, 1602, 1932, 2076
MaeII	ACGT	3 cut(s)	708, 1326, 1430
MbiI	CCGCTC	1 cut(s)	1053
MboII	GAAGA	8 cut(s)	17, 59, 503, 678, 1956, 2208, 2209, 2212
MflI	RGATCY	2 cut(s)	910, 1709
MhlI	GDGCHC	4 cut(s)	592, 1837, 2227, 2270
MluI	ACGCGT	1 cut(s)	1907
MlyI	GAGTC	1 cut(s)	295
MmeI	TCCRAC	2 cut(s)	523, 1447
MroNI	GCCGGC	1 cut(s)	1037
MseI	TTAA	3 cut(s)	600, 1070, 1088
MslI	CAYNNNNRTG	3 cut(s)	12, 75, 1944
MspA1I	CMGCKG	4 cut(s)	695, 1457, 1571, 2072
MspR9I	CCNGG	9 cut(s)	317, 318, 403, 627, 1500, 1529, 1530, 1624, 1845
MvnI	CGCG	4 cut(s)	1314, 1909, 1994, 2072
MwoI	GCNNNNNNNGC	7 cut(s)	676, 767, 776, 932, 1205, 1457, 1808
NaeI	GCCGGC	1 cut(s)	1039
NciI	CCSGG	9 cut(s)	317, 318, 403, 627, 1500, 1529, 1530, 1624, 1845
NcoI	CCATGG	2 cut(s)	939, 1632
NdeI	CATATG	1 cut(s)	1217
NgoMIV	GCCGGC	1 cut(s)	1037
NlaIV	GGNNCC	6 cut(s)	21, 912, 985, 1527, 1557, 2267
NmeAIII	GCCGAG	1 cut(s)	629
NmuCI	GTSAC	7 cut(s)	298, 631, 1322, 1772, 1814, 2176, 2280
NsbI	TGCGCA	1 cut(s)	1680
NspI	RCATGY	2 cut(s)	1173, 1641
PaeI	GCATGC	2 cut(s)	1173, 1641
PciSI	GCTCTTC	1 cut(s)	72
PdiI	GCCGGC	1 cut(s)	1039
PfeI	GAWTC	1 cut(s)	179
PfoI	TCCNGGA	1 cut(s)	1622
PinAI	ACCGGT	1 cut(s)	458
PleI	GAGTC	1 cut(s)	295
PpsI	GAGTC	1 cut(s)	295
Ppu21I	YACGTR	1 cut(s)	709
PpuMI	RGGWCCY	1 cut(s)	1481
Psp124BI	GAGCTC	1 cut(s)	592
Psp1406I	AACGTT	1 cut(s)	1430
Psp5II	RGGWCCY	1 cut(s)	1481
PspN4I	GGNNCC	6 cut(s)	21, 912, 985, 1527, 1557, 2267
PspPI	GGNCC	6 cut(s)	838, 983, 1481, 1525, 1846, 2046
PspPPI	RGGWCCY	1 cut(s)	1481
PstI	CTGCAG	1 cut(s)	700
PsuI	RGATCY	2 cut(s)	910, 1709
PvuII	CAGCTG	1 cut(s)	695
RsaI	GTAC	7 cut(s)	164, 242, 785, 905, 1382, 2000, 2081
RsaNI	GTAC	7 cut(s)	163, 241, 784, 904, 1381, 1999, 2080
RseI	CAYNNNNRTG	3 cut(s)	12, 75, 1944
SacI	GAGCTC	1 cut(s)	592
SacII	CCGCGG	1 cut(s)	2073
SalI	GTCGAC	1 cut(s)	97
SapI	GCTCTTC	1 cut(s)	72
SaqAI	TTAA	3 cut(s)	600, 1070, 1088
Sau96I	GGNCC	6 cut(s)	838, 983, 1481, 1525, 1846, 2046
SchI	GAGTC	1 cut(s)	295
ScrFI	CCNGG	9 cut(s)	317, 318, 403, 627, 1500, 1529, 1530, 1624, 1845
SduI	GDGCHC	4 cut(s)	592, 1837, 2227, 2270
SfaNI	GCATC	6 cut(s)	728, 1020, 1195, 1690, 1849, 2056
SfcI	CTRYAG	2 cut(s)	696, 915
Sfr303I	CCGCGG	1 cut(s)	2073
SgrBI	CCGCGG	1 cut(s)	2073
SinI	GGWCC	3 cut(s)	838, 1481, 2046
SmaI	CCCGGG	2 cut(s)	318, 1530
SmiMI	CAYNNNNRTG	3 cut(s)	12, 75, 1944
SnaBI	TACGTA	1 cut(s)	709
SphI	GCATGC	2 cut(s)	1173, 1641
SsiI	CCGC	8 cut(s)	4, 1051, 1182, 1366, 1455, 1571, 2070, 2072
SspMI	CTAG	9 cut(s)	147, 519, 654, 827, 1190, 1338, 1602, 1932, 2076
SstI	GAGCTC	1 cut(s)	592
StyD4I	CCNGG	9 cut(s)	315, 316, 401, 625, 1498, 1527, 1528, 1622, 1843
StyI	CCWWGG	3 cut(s)	939, 1016, 1632
TaiI	ACGT	3 cut(s)	711, 1329, 1433
TatI	WGTACW	3 cut(s)	240, 783, 1998
TauI	GCSGC	3 cut(s)	7, 2072, 2075
TfiI	GAWTC	1 cut(s)	179
Tru1I	TTAA	3 cut(s)	600, 1070, 1088
Tru9I	TTAA	3 cut(s)	600, 1070, 1088
TscAI	CASTG	4 cut(s)	277, 667, 1234, 2263
TseFI	GTSAC	7 cut(s)	298, 631, 1322, 1772, 1814, 2176, 2280
Tsp45I	GTSAC	7 cut(s)	298, 631, 1322, 1772, 1814, 2176, 2280
TspGWI	ACGGA	1 cut(s)	441
TspMI	CCCGGG	2 cut(s)	316, 1528
TspRI	CASTG	4 cut(s)	277, 667, 1234, 2263
VneI	GTGCAC	1 cut(s)	2223
VpaK11BI	GGWCC	3 cut(s)	838, 1481, 2046
XapI	RAATTY	2 cut(s)	1417, 1784
XceI	RCATGY	2 cut(s)	1173, 1641
XmaI	CCCGGG	2 cut(s)	316, 1528
XmiI	GTMKAC	2 cut(s)	98, 711
XspI	CTAG	9 cut(s)	147, 519, 654, 827, 1190, 1338, 1602, 1932, 2076

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.