Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0001268

Physical Location & Seq

Genomic Coordinates Reverse (-)

15943380 .. 15945911

Gene Structure 2532 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G014280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGGCTACTCTAGCTGTACTAGTTCTATGCTCAATTCTAATCTCAATCATGGCTTCTGCACAATCTCACACATACATTGTCCACATGGATTCTTCAGCCATGCCCAAAGCCTTCTTTGATCACCACAAATGGTATTTGGCAACCCTTTCATCTCTATCAGACGGTGTCAACACTGAGGCAACTACCAGTACTACCACAAAGCTCATTTATACCTATACTAATGCAATTCAAGGCTTCAGTGCAACTCTCACACTTTCTGAGCTTGAATCCCTCAAAAGCTCCCTTGGCTTCATTTCATATATTCGTGATCGCCCCCTTAAACTTCTCACCACCCGCTCTTCTCGGTTCCTTGGTCTAACACCCTTGTTTGGCGCTTGGCCTGCTTCGAATTATGGTGAGAATGTCACAATTGGTGTAATTGACACCGGAGTTTGGCCCGAGAGTGAGAGCTTCAATGATGAAGGAATGACTCAAATCCCCTCTAGATGGAAAGGAAAATGTGAATCCGGCACCCAATTTAGCTCTTCCTTGTGCAACAAGAAACTCATTGGGGCCCGGTTTTACTATAAAGGCTTTATTGCAGAGAATCCAGACTTAAATATATCAATGAAGTCAGCCCGCGATACAAATGGACATGGAACACACACTTCCTCTATTGCTGCTGGCAATTATGTAAAGGGCGCATCCTATTTTGGCTATGCTACTGGCACAGCTGTAGGCGTGGCACCAAGAGCTCGAATAGCAATGTACAAAGTGGGTTGGAATGGGGAGGTATCCGGATCTGATGTTATTGCTGCCGTAGACCAATCAATTCAAGATGGGGTGGACATATTATCTCTTTCCTTGGGTTATAGCTTGGATGAATATTTGTATCATGACATAATTGCAATAGCGACTTTTGCAGCCATGAATAAGGGAATTTTTGTTGCAGCTGCAGCCGGAAATGATGGCCCTGGCTATGGTACCTTAGTCAATGGAGCACCATGGGCAGTGATAGTTGGTGCAGGAACTATCGATCGCCAGCTCGGAGGAATTTTAACCTTGGGAAATGGTATGAAAATCACTTTCCCATCCTTGTATCCAAGGAACTTCTCTAGAGATACTCAGTTGCCACTTGTTTTTAATGGCTGTCAAAGTGTGATGGAACTGAGGAAACTCAAAGGCAAGATTGTTGTTTGCAAAGACAATATGAGCATAGATGTTCAAGTTGAGAATGCAAAGTCTGCAAAGGTTTCAGGAGCTGTTTTCATATCCAATACTACCTTGTCAGATTATGATACCATAACCATATTTCCAGCGGCAGTTATTGGTTACCAAGATGGCAAACTGGTAGTAGACTATATTAAGCAAAGTAGTAATCCTAAAGCAATTTTGGAATTCAGGAAAACAGTCATTGGTACAAAACCTGCACCAAGGGTTGACGATTACAGTTCCAGAGGGCCATTTCCAAGCTGCCCTACAATTCTGAAGCCAGACATTTTAGCCCCTGGATCATTAATCTTGGCATCATGGTCCCCAACTAGTTCAGTATCTGAAGATCAGTCAAGCCCTTTGTTTAGTAACTTCAATCTTGACACAGGGACTTCAATGTCAGCGCCTCATGTGGCCGGTGTGGCTGCGCTTATCAAATCTGTGCATCCTGATTGGAGCCCTGCGGCTATTAGATCAGCTCTTATGACCACGGCGAATCCATTAGACAACACTCACAGCCCTATCAAAGATGCTTACGGAAACCTCATTGCAAGCCCTTTAGCCATTGGAGCAGGACACATCAATCCCAACAAGGCTCTTGATCCAGGACTAGTCTATGATGCAGCAGCAGATGACTACATAAAGCTTCTATGTGCAATGAAATACACAGCAAAGCAGATACAAATTATCACAGGGTCCATTTATAAATGTGCCAATAGGTTCAACATTGATATTAATTACCCGTCTTTCATTGCTTACTTTAACAGTAACGGGTCAAATTCGGATGCAAAAGTTGTGCAAGAGTTCAGGAGGACACTAACAAATGTGGGAGAGGAACAGTCCAGCTACATTGTGAATCTTACAGCCTTGGCTGGTTTAAAATTGAAGGTGGAGCCACAGAGGTTGGTGTTCACAAAGAAGTATGAGAAGCTAAGGTACAAGCTGACTATGGTAGGTCCAAAATTGTTGAAAGAAGATTTGGTTCAGGGCTCACTTAGTTGGATTGATGATGGTGGAAAATATGTAGTGAGAAGTCCAATAGTGGCCACAAACATAGTGCCAGATTCTCTGATAAGGGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

757

Amino Acids

81.61

Weight (kDa)

8.48

Isoelectric Point (pI)

30.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	24 - 108	3.3e-14	Peptidase inhibitor I9
Peptidase_S8	PF00082	132 - 568	4.9e-49	Subtilase family
PA	PF02225	370 - 442	3.9e-06	PA domain
fn3_6	PF17766	641 - 745	9.4e-23	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001268)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67090
fragaria_vesca	FvH4_1g16180 FvH4_2g34170 FvH4_2g34170 FvH4_2g34230 FvH4_6g01060 FvH4_6g01061
malus_domestica	MD04G1236800.v1.1 MD04G1236900.v1.1 MD08G1098100.v1.1 MD15G1080600.v1.1 MD15G1285300.v1.1
prunus_persica	Prupe.1G433300_v2.0.a1 Prupe.6G168400_v2.0.a1 Prupe.6G356800_v2.0.a1 Prupe.7G131900_v2.0.a1
pyrus_communis	pycom04g21060 pycom04g21070 pycom08g07990 pycom12g23460 pycom15g07490 pycom15g24800
rosa_chinensis	RchiOBHm_Chr2g0105861 RchiOBHm_Chr2g0105881 RchiOBHm_Chr3g0448571 RchiOBHm_Chr6g0308191
rosa_laevigata	RLG00000010660 RLG00000017466 RLG00000017467 RLG00000025886 RLG00000025888 RLG00000025889 RLG00000025890
rosa_multiflora	Rmu_sc0003116.1_g000043 Rmu_sc0004367.1_g000007 Rmu_sc0004367.1_g000008 Rmu_sc0005706.1_g000016 Rmu_sc0010389.1_g000023
rosa_roxburghii	Rroxscaffold_2G00137780 Rroxscaffold_6G00426820 Rroxscaffold_6G00426860 Rroxscaffold_7G00160370 Rroxscaffold_7G00160460
rosa_rugosa	Rorug02G0131300 Rorug02G0131500 Rorug02G0613500 Rorug02G0613600 Rorug06G0364600
rosa_samantha	Rh2CG186700 Rh3DG013500 Rh6DG475800
rosa_wichuraiana	Rw2G014280 Rw2G014290 Rw3G000970 Rw6G041320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1896
AasI	GACNNNNNNGTC	1 cut(s)	1588
Acc36I	ACCTGC	1 cut(s)	1414
Acc65I	GGTACC	1 cut(s)	962
AccB1I	GGYRCC	3 cut(s)	509, 724, 962
AccBSI	CCGCTC	1 cut(s)	336
AccI	GTMKAC	2 cut(s)	801, 1335
AccII	CGCG	1 cut(s)	621
AccIII	TCCGGA	1 cut(s)	776
AciI	CCGC	4 cut(s)	334, 619, 1298, 1655
AclWI	GGATC	3 cut(s)	787, 1498, 1787
AcoI	YGGCCR	2 cut(s)	1605, 2235
AcsI	RAATTY	4 cut(s)	918, 1032, 1376, 1969
AcuI	CTGAAG	4 cut(s)	78, 220, 1487, 1554
AfaI	GTAC	6 cut(s)	18, 190, 749, 964, 1399, 2129
AfiI	CCNNNNNNNGG	4 cut(s)	368, 432, 486, 959
AhlI	ACTAGT	3 cut(s)	19, 1520, 1801
AjnI	CCWGG	3 cut(s)	952, 1486, 1795
AjuI	GAANNNNNNNTTGG	2 cut(s)	2153, 2185
Alw21I	GWGCWC	2 cut(s)	736, 982
AlwI	GGATC	3 cut(s)	787, 1498, 1787
AlwNI	CAGNNNCTG	2 cut(s)	1241, 1532
Ama87I	CYCGRG	1 cut(s)	437
Aor13HI	TCCGGA	1 cut(s)	776
AoxI	GGCC	7 cut(s)	377, 434, 552, 949, 1439, 1605, 2235
ApaI	GGGCCC	1 cut(s)	556
ApoI	RAATTY	4 cut(s)	918, 1032, 1376, 1969
AseI	ATTAAT	2 cut(s)	1496, 1926
Asp718I	GGTACC	1 cut(s)	962
AspLEI	GCGC	4 cut(s)	374, 683, 1597, 1621
AspS9I	GGNCC	8 cut(s)	435, 552, 553, 950, 1439, 1512, 1887, 2147
AsuC2I	CCSGG	1 cut(s)	556
AsuHPI	GGTGA	3 cut(s)	113, 319, 407
AsuII	TTCGAA	1 cut(s)	386
AvaI	CYCGRG	1 cut(s)	437
AvaII	GGWCC	3 cut(s)	1512, 1887, 2147
BaeGI	GKGCMC	1 cut(s)	556
BaeI	ACNNNNGTAYC	2 cut(s)	954, 987
BalI	TGGCCA	1 cut(s)	2237
BanI	GGYRCC	3 cut(s)	509, 724, 962
BanII	GRGCYC	4 cut(s)	556, 736, 1652, 2183
BarI	GAAGNNNNNNTAC	2 cut(s)	2111, 2143
Bbv12I	GWGCWC	2 cut(s)	736, 982
BccI	CCATC	7 cut(s)	480, 812, 941, 1078, 1135, 1313, 2195
BceAI	ACGGC	2 cut(s)	782, 1698
BciT130I	CCWGG	3 cut(s)	954, 1488, 1797
BciVI	GTATCC	2 cut(s)	784, 1089
BclI	TGATCA	1 cut(s)	118
BcnI	CCSGG	1 cut(s)	556
BcuI	ACTAGT	3 cut(s)	19, 1520, 1801
BfaI	CTAG	6 cut(s)	11, 20, 483, 1095, 1521, 1802
BfmI	CTRYAG	2 cut(s)	714, 933
BfoI	RGCGCY	2 cut(s)	375, 1598
BfuAI	ACCTGC	1 cut(s)	1414
BfuI	GTATCC	2 cut(s)	784, 1089
BglI	GCCNNNNNGGC	1 cut(s)	1613
BmcAI	AGTACT	1 cut(s)	190
Bme1390I	CCNGG	4 cut(s)	556, 954, 1488, 1797
Bme18I	GGWCC	3 cut(s)	1512, 1887, 2147
BmeT110I	CYCGRG	1 cut(s)	437
BmgT120I	GGNCC	8 cut(s)	435, 552, 553, 950, 1439, 1512, 1887, 2147
BmrFI	CCNGG	4 cut(s)	556, 954, 1488, 1797
BmsI	GCATC	6 cut(s)	692, 1514, 1645, 1711, 1801, 1966
Bpu10I	CCTNAGC	1 cut(s)	2123
Bpu14I	TTCGAA	1 cut(s)	386
BpuMI	CCSGG	1 cut(s)	556
Bsa29I	ATCGAT	1 cut(s)	1014
BsaWI	WCCGGW	2 cut(s)	425, 776
Bsc4I	CCNNNNNNNGG	4 cut(s)	368, 432, 486, 959
Bse118I	RCCGGY	1 cut(s)	1607
Bse1I	ACTGG	3 cut(s)	186, 709, 1332
Bse3DI	GCAATG	4 cut(s)	750, 1737, 1854, 1941
BseAI	TCCGGA	1 cut(s)	776
BseBI	CCWGG	3 cut(s)	954, 1488, 1797
BseCI	ATCGAT	1 cut(s)	1014
BseGI	GGATG	5 cut(s)	683, 865, 1070, 1636, 1981
BseLI	CCNNNNNNNGG	4 cut(s)	368, 432, 486, 959
BseMI	GCAATG	4 cut(s)	750, 1737, 1854, 1941
BseMII	CTCAG	4 cut(s)	165, 249, 1118, 1139
BseNI	ACTGG	3 cut(s)	186, 709, 1332
BseSI	GKGCMC	1 cut(s)	556
BsgI	GTGCAG	3 cut(s)	42, 1023, 1392
Bsh1236I	CGCG	1 cut(s)	621
Bsh1285I	CGRYCG	1 cut(s)	1018
BshFI	GGCC	7 cut(s)	379, 436, 554, 951, 1441, 1607, 2237
BshNI	GGYRCC	3 cut(s)	509, 724, 962
BshVI	ATCGAT	1 cut(s)	1014
BsiEI	CGRYCG	1 cut(s)	1018
BsiHKAI	GWGCWC	2 cut(s)	736, 982
BsiHKCI	CYCGRG	1 cut(s)	437
BsiSI	CCGG	6 cut(s)	426, 507, 556, 777, 939, 1608
BslFI	GGGAC	2 cut(s)	1498, 1594
BslI	CCNNNNNNNGG	4 cut(s)	368, 432, 486, 959
BsmFI	GGGAC	2 cut(s)	1498, 1594
BsmI	GAATGC	1 cut(s)	1219
BsnI	GGCC	7 cut(s)	379, 436, 554, 951, 1441, 1607, 2237
BsoBI	CYCGRG	1 cut(s)	437
Bsp119I	TTCGAA	1 cut(s)	386
Bsp120I	GGGCCC	1 cut(s)	552
Bsp1286I	GDGCHC	5 cut(s)	556, 736, 982, 1652, 2183
Bsp13I	TCCGGA	1 cut(s)	776
Bsp1407I	TGTACA	1 cut(s)	747
Bsp143I	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
Bsp19I	CCATGG	1 cut(s)	983
BspACI	CCGC	4 cut(s)	334, 619, 1298, 1655
BspANI	GGCC	7 cut(s)	379, 436, 554, 951, 1441, 1607, 2237
BspCNI	CTCAG	4 cut(s)	166, 250, 1117, 1140
BspDI	ATCGAT	1 cut(s)	1014
BspEI	TCCGGA	1 cut(s)	776
BspFNI	CGCG	1 cut(s)	621
BspHI	TCATGA	1 cut(s)	874
BspMAI	CTGCAG	1 cut(s)	937
BspMI	ACCTGC	1 cut(s)	1414
BspPI	GGATC	3 cut(s)	787, 1498, 1787
BspQI	GCTCTTC	2 cut(s)	343, 529
BspT104I	TTCGAA	1 cut(s)	386
BspT107I	GGYRCC	3 cut(s)	509, 724, 962
BsrBI	CCGCTC	1 cut(s)	336
BsrDI	GCAATG	4 cut(s)	750, 1737, 1854, 1941
BsrFI	RCCGGY	1 cut(s)	1607
BsrGI	TGTACA	1 cut(s)	747
BsrI	ACTGG	3 cut(s)	186, 709, 1332
BssAI	RCCGGY	1 cut(s)	1607
BssMI	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
BssT1I	CCWWGG	8 cut(s)	283, 349, 843, 983, 1041, 1082, 1412, 2058
Bst2UI	CCWGG	3 cut(s)	954, 1488, 1797
Bst4CI	ACNGT	5 cut(s)	164, 1390, 1430, 1958, 2031
Bst6I	CTCTTC	2 cut(s)	343, 529
BstAPI	GCANNNNNTGC	1 cut(s)	1223
BstAUI	TGTACA	1 cut(s)	747
BstBI	TTCGAA	1 cut(s)	386
BstC8I	GCNNGC	5 cut(s)	381, 619, 664, 1022, 1744
BstDEI	CTNAG	7 cut(s)	174, 258, 967, 1104, 1148, 2123, 2186
BstDSI	CCRYGG	2 cut(s)	983, 1680
BstEII	GGTNACC	1 cut(s)	1310
BstF5I	GGATG	5 cut(s)	683, 865, 1070, 1636, 1981
BstFNI	CGCG	1 cut(s)	621
BstH2I	RGCGCY	2 cut(s)	375, 1598
BstHHI	GCGC	4 cut(s)	374, 683, 1597, 1621
BstKTI	GATC	8 cut(s)	121, 310, 782, 1018, 1493, 1540, 1667, 1795
BstMBI	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
BstMCI	CGRYCG	1 cut(s)	1018
BstNI	CCWGG	3 cut(s)	954, 1488, 1797
BstPI	GGTNACC	1 cut(s)	1310
BstSCI	CCNGG	4 cut(s)	554, 952, 1486, 1795
BstSFI	CTRYAG	2 cut(s)	714, 933
BstSLI	GKGCMC	1 cut(s)	556
BstUI	CGCG	1 cut(s)	621
BstX2I	RGATCY	1 cut(s)	779
BstYI	RGATCY	1 cut(s)	779
Bsu15I	ATCGAT	1 cut(s)	1014
BsuI	GTATCC	2 cut(s)	784, 1089
BsuRI	GGCC	7 cut(s)	379, 436, 554, 951, 1441, 1607, 2237
BsuTUI	ATCGAT	1 cut(s)	1014
BtgI	CCRYGG	2 cut(s)	983, 1680
BtsCI	GGATG	5 cut(s)	683, 865, 1070, 1636, 1981
BtsI	GCAGTG	1 cut(s)	996
BtsIMutI	CAGTG	3 cut(s)	171, 244, 996
BveI	ACCTGC	1 cut(s)	1414
Cac8I	GCNNGC	5 cut(s)	381, 619, 664, 1022, 1744
CaiI	CAGNNNCTG	2 cut(s)	1241, 1532
CciI	TCATGA	1 cut(s)	874
CfoI	GCGC	4 cut(s)	374, 683, 1597, 1621
Cfr10I	RCCGGY	1 cut(s)	1607
Cfr13I	GGNCC	8 cut(s)	435, 552, 553, 950, 1439, 1512, 1887, 2147
ClaI	ATCGAT	1 cut(s)	1014
Csp6I	GTAC	6 cut(s)	17, 189, 748, 963, 1398, 2128
CspCI	CAANNNNNGTGG	2 cut(s)	2076, 2111
CviAII	CATG	9 cut(s)	49, 85, 100, 635, 875, 907, 984, 1509, 1601
CviQI	GTAC	6 cut(s)	17, 189, 748, 963, 1398, 2128
DdeI	CTNAG	7 cut(s)	174, 258, 967, 1104, 1148, 2123, 2186
DpnI	GATC	8 cut(s)	120, 309, 781, 1017, 1492, 1539, 1666, 1794
DpnII	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
DraI	TTTAAA	1 cut(s)	2070
DrdI	GACNNNNNNGTC	1 cut(s)	1588
DseDI	GACNNNNNNGTC	1 cut(s)	1588
EaeI	YGGCCR	2 cut(s)	1605, 2235
Eam1104I	CTCTTC	2 cut(s)	343, 529
EarI	CTCTTC	2 cut(s)	343, 529
Ecl136II	GAGCTC	1 cut(s)	734
Eco130I	CCWWGG	8 cut(s)	283, 349, 843, 983, 1041, 1082, 1412, 2058
Eco24I	GRGCYC	4 cut(s)	556, 736, 1652, 2183
Eco47I	GGWCC	3 cut(s)	1512, 1887, 2147
Eco53kI	GAGCTC	1 cut(s)	734
Eco57I	CTGAAG	4 cut(s)	78, 220, 1487, 1554
Eco88I	CYCGRG	1 cut(s)	437
Eco91I	GGTNACC	1 cut(s)	1310
EcoICRI	GAGCTC	1 cut(s)	734
EcoO109I	RGGNCCY	1 cut(s)	552
EcoO65I	GGTNACC	1 cut(s)	1310
EcoRI	GAATTC	1 cut(s)	1376
EcoRII	CCWGG	3 cut(s)	952, 1486, 1795
EcoT14I	CCWWGG	8 cut(s)	283, 349, 843, 983, 1041, 1082, 1412, 2058
EcoT38I	GRGCYC	4 cut(s)	556, 736, 1652, 2183
ErhI	CCWWGG	8 cut(s)	283, 349, 843, 983, 1041, 1082, 1412, 2058
FaeI	CATG	9 cut(s)	52, 88, 103, 638, 878, 910, 987, 1512, 1604
FaqI	GGGAC	2 cut(s)	1498, 1594
FatI	CATG	9 cut(s)	48, 84, 99, 634, 874, 906, 983, 1508, 1600
FauI	CCCGC	2 cut(s)	341, 626
FbaI	TGATCA	1 cut(s)	118
FblI	GTMKAC	2 cut(s)	801, 1335
FokI	GGATG	5 cut(s)	670, 872, 1057, 1623, 1988
FriOI	GRGCYC	4 cut(s)	556, 736, 1652, 2183
FspBI	CTAG	6 cut(s)	11, 20, 483, 1095, 1521, 1802
GlaI	GCGC	4 cut(s)	373, 682, 1596, 1620
HaeII	RGCGCY	2 cut(s)	375, 1598
HaeIII	GGCC	7 cut(s)	379, 436, 554, 951, 1441, 1607, 2237
HapII	CCGG	6 cut(s)	426, 507, 556, 777, 939, 1608
HhaI	GCGC	4 cut(s)	374, 683, 1597, 1621
Hin1II	CATG	9 cut(s)	52, 88, 103, 638, 878, 910, 987, 1512, 1604
Hin6I	GCGC	4 cut(s)	372, 681, 1595, 1619
HinP1I	GCGC	4 cut(s)	372, 681, 1595, 1619
HincII	GTYRAC	2 cut(s)	169, 1420
HindII	GTYRAC	2 cut(s)	169, 1420
HindIII	AAGCTT	1 cut(s)	1835
HinfI	GANTC	8 cut(s)	89, 266, 469, 503, 586, 1687, 2047, 2255
HpaII	CCGG	6 cut(s)	426, 507, 556, 777, 939, 1608
HphI	GGTGA	3 cut(s)	113, 319, 407
Hpy166II	GTNNAC	7 cut(s)	82, 169, 802, 826, 1336, 1420, 2103
Hpy188I	TCNGA	9 cut(s)	160, 259, 784, 1028, 1270, 1467, 1534, 1975, 2262
Hpy8I	GTNNAC	7 cut(s)	82, 169, 802, 826, 1336, 1420, 2103
HpyAV	CCTTC	3 cut(s)	121, 455, 2071
HpyCH4III	ACNGT	5 cut(s)	164, 1390, 1430, 1958, 2031
HpyF3I	CTNAG	7 cut(s)	174, 258, 967, 1104, 1148, 2123, 2186
Hsp92II	CATG	9 cut(s)	52, 88, 103, 638, 878, 910, 987, 1512, 1604
HspAI	GCGC	4 cut(s)	372, 681, 1595, 1619
Kpn2I	TCCGGA	1 cut(s)	776
KpnI	GGTACC	1 cut(s)	966
Ksp22I	TGATCA	1 cut(s)	118
Kzo9I	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
LguI	GCTCTTC	2 cut(s)	343, 529
LmnI	GCTCC	6 cut(s)	284, 977, 1238, 1647, 1760, 2083
LweI	GCATC	6 cut(s)	692, 1514, 1645, 1711, 1801, 1966
MaeI	CTAG	6 cut(s)	11, 20, 483, 1095, 1521, 1802
MaeIII	GTNAC	4 cut(s)	403, 1310, 1559, 1958
MalI	GATC	8 cut(s)	120, 309, 781, 1017, 1492, 1539, 1666, 1794
MbiI	CCGCTC	1 cut(s)	336
MboI	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
MboII	GAAGA	5 cut(s)	84, 330, 516, 1547, 2177
MfeI	CAATTG	1 cut(s)	408
MflI	RGATCY	1 cut(s)	779
MhlI	GDGCHC	5 cut(s)	556, 736, 982, 1652, 2183
MlsI	TGGCCA	1 cut(s)	2237
MluNI	TGGCCA	1 cut(s)	2237
MlyI	GAGTC	1 cut(s)	463
MmeI	TCCRAC	2 cut(s)	740, 2171
Mox20I	TGGCCA	1 cut(s)	2237
MroI	TCCGGA	1 cut(s)	776
MscI	TGGCCA	1 cut(s)	2237
Msp20I	TGGCCA	1 cut(s)	2237
MspA1I	CMGCKG	3 cut(s)	713, 932, 1298
MspI	CCGG	6 cut(s)	426, 507, 556, 777, 939, 1608
MspR9I	CCNGG	4 cut(s)	556, 954, 1488, 1797
MunI	CAATTG	1 cut(s)	408
Mva1269I	GAATGC	1 cut(s)	1219
MvaI	CCWGG	3 cut(s)	954, 1488, 1797
MvnI	CGCG	1 cut(s)	621
NciI	CCSGG	1 cut(s)	556
NcoI	CCATGG	1 cut(s)	983
NdeII	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
NlaIII	CATG	9 cut(s)	52, 88, 103, 638, 878, 910, 987, 1512, 1604
NmuCI	GTSAC	1 cut(s)	403
NspV	TTCGAA	1 cut(s)	386
PagI	TCATGA	1 cut(s)	874
PciSI	GCTCTTC	2 cut(s)	343, 529
PctI	GAATGC	1 cut(s)	1219
PfeI	GAWTC	7 cut(s)	89, 266, 503, 586, 1687, 2047, 2255
PflFI	GACNNNGTC	1 cut(s)	164
PfoI	TCCNGGA	1 cut(s)	1795
Ple19I	CGATCG	1 cut(s)	1018
PleI	GAGTC	1 cut(s)	463
PpsI	GAGTC	1 cut(s)	463
PshBI	ATTAAT	2 cut(s)	1496, 1926
PsiI	TTATAA	1 cut(s)	1896
Psp124BI	GAGCTC	1 cut(s)	736
Psp6I	CCWGG	3 cut(s)	952, 1486, 1795
PspEI	GGTNACC	1 cut(s)	1310
PspGI	CCWGG	3 cut(s)	952, 1486, 1795
PspOMI	GGGCCC	1 cut(s)	552
PspPI	GGNCC	8 cut(s)	435, 552, 553, 950, 1439, 1512, 1887, 2147
PstI	CTGCAG	1 cut(s)	937
PstNI	CAGNNNCTG	2 cut(s)	1241, 1532
PsuI	RGATCY	1 cut(s)	779
PsyI	GACNNNGTC	1 cut(s)	164
PvuI	CGATCG	1 cut(s)	1018
PvuII	CAGCTG	2 cut(s)	713, 932
RsaI	GTAC	6 cut(s)	18, 190, 749, 964, 1399, 2129
RsaNI	GTAC	6 cut(s)	17, 189, 748, 963, 1398, 2128
SacI	GAGCTC	1 cut(s)	736
SapI	GCTCTTC	2 cut(s)	343, 529
Sau3AI	GATC	8 cut(s)	118, 307, 779, 1015, 1490, 1537, 1664, 1792
Sau96I	GGNCC	8 cut(s)	435, 552, 553, 950, 1439, 1512, 1887, 2147
ScaI	AGTACT	1 cut(s)	190
SchI	GAGTC	1 cut(s)	463
ScrFI	CCNGG	4 cut(s)	556, 954, 1488, 1797
SduI	GDGCHC	5 cut(s)	556, 736, 982, 1652, 2183
SfaNI	GCATC	6 cut(s)	692, 1514, 1645, 1711, 1801, 1966
SfcI	CTRYAG	2 cut(s)	714, 933
SfuI	TTCGAA	1 cut(s)	386
SinI	GGWCC	3 cut(s)	1512, 1887, 2147
SpeI	ACTAGT	3 cut(s)	19, 1520, 1801
SsiI	CCGC	4 cut(s)	334, 619, 1298, 1655
SspI	AATATT	1 cut(s)	866
SspMI	CTAG	6 cut(s)	11, 20, 483, 1095, 1521, 1802
SstI	GAGCTC	1 cut(s)	736
StyD4I	CCNGG	4 cut(s)	554, 952, 1486, 1795
StyI	CCWWGG	8 cut(s)	283, 349, 843, 983, 1041, 1082, 1412, 2058
TaaI	ACNGT	5 cut(s)	164, 1390, 1430, 1958, 2031
TaqI	TCGA	3 cut(s)	386, 736, 1014
TatI	WGTACW	3 cut(s)	16, 188, 747
TauI	GCSGC	2 cut(s)	1301, 1658
TfiI	GAWTC	7 cut(s)	89, 266, 503, 586, 1687, 2047, 2255
TscAI	CASTG	3 cut(s)	178, 244, 996
TseFI	GTSAC	1 cut(s)	403
Tsp45I	GTSAC	1 cut(s)	403
TspGWI	ACGGA	1 cut(s)	1743
TspRI	CASTG	3 cut(s)	178, 244, 996
Tth111I	GACNNNGTC	1 cut(s)	164
VpaK11BI	GGWCC	3 cut(s)	1512, 1887, 2147
VspI	ATTAAT	2 cut(s)	1496, 1926
XapI	RAATTY	4 cut(s)	918, 1032, 1376, 1969
XbaI	TCTAGA	2 cut(s)	482, 1094
XmiI	GTMKAC	2 cut(s)	801, 1335
XspI	CTAG	6 cut(s)	11, 20, 483, 1095, 1521, 1802
ZrmI	AGTACT	1 cut(s)	190

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.