RchiOBHm_Chr6g0308191

subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001268
Physical Location & Seq
Reverse (-)
66300228 .. 66302774
2547 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27709

Sequence Viewer

Length: 2286 bp
ATGGCTACTCATAACCATCATGTTCCCTTGTACTTGTGGCTTTCCATTGTCACAATCTCAAACCTTGCCAAAGTCTCCACCTTGGCTCATGAATCCAACAATTATATCATCCACATGGATTCATCATTTATGCCCAAAGCCTTTGCAGATCACCATTCTTGGTACTTGTCCACTGTAGATTCTGTACTATCCACTTCTTCTATCTCTTCTAAGCTCATTTATAGCTACACTCATGTCTTAAACGGGTTCAGTGCAAGTCTCTCTGTTCCTGAGCTGGAGGCCTTAAAAACCTCTCCAGGGTACATATCTTCAGTTAGAGACCTGCCGGTAAAACGCGACACGACCCACTCGTCCCAGTTTCTTGGCCTTAACTCCAAGACGGGAGCATGGCCGGTGTCCAATTACGGCAAAGGTACCATTATTGGTTTGGTGGACACCGGGGTTTGGCCAGAAAACGAAAGCTTTAACGACGCTGGAATGTCAGAAATCCCACCAAGATGGAAAGGAGAATGTGAAAGTGGTACACAATTCAGCTCTTCATTGTGCAACAAAAAGCTCATTGGGGCAAGGTTCTTTAACAAAGGAATCGTAGCCCGACAGCCCAATGTGACATTCACCATGAACTCCACAAGGGACACCGACGGTCATGGGACGCACACCTCTTCTACGGCCGCTGGAAACTATGTGGCCGGGGCATCGTGCTTTAATTATGCTCCGGGAACTGCCAGCGGCATGGCGCCTCGGGCTCACGTGGCCATGTACAAGGCTCTGTGGGACGAGGGTGCCTTATCGTCTGACATAATTGCTGCCATCGAGCAAGCAGTTGTGGACGGTGTGGATGTCTTATCCCTGTCGTTCGGATTAGATGGCGTTGCGCTGTACGAGGACCCTGTTGCCATAGCCACATTCTCAGCCATCGAGAAGGGCGTCTTCGTTTCCACTTCGGCAGGAAATGAAGGACCTGTCTATGGTAGCTTGCATAATGGAATCCCTTGGGTTCTAACAGTTGCAGCTAGTACCATTGATCGTGACTTTCAGGGAACTGCACATCTTGGAAATGGAGAATCCGTCACAGGGATGACTCTTTTTCCGGGGGTTAATTCGTCTTCAACTCCAATCCCAATTGTTTTCATTAATGCATGTGACAATTTGAAGATGCTGAACAAGGTTGGGAATAAAATCGTGTTGTGCCAGGACAACAATTCACTTGAGGAACAATATCCTACTGTTCAAGATGCAAATGTTGCCGGAGGTATCTTCATTACCAACAACACCGACTTGGAATTGTTCCTCCAGAGCCAATTTCCCACACTTTTTTTGAGTCCAAAGGAAGGTGAAACTATCAAAGACTACATCAAGAGCAATTCTAACCCGAAAGTAAGCTTGGAGTTTCAAAAGACACTGCTTGGTGTGAGACCAGCACCGACGGTTACTAGTTACACATCTAGAGGTCCATCTTCCAGCTTCCCCTTTACTCTCAAGCCGGACATTACTGCACCGGGTTCACTAATCCTCGCTGCATGGCCTCGAAACATTTCGGTGGCAACGATTAACAAAAAACAGGTTCTGTATAGTGAATTCAATTTGTTGTCGGGGACATCCATGGCGTGCCCACATGCAGCTGGGTTAGCTGCGCTTGTGAAAGGTGCACATCCTGAATGGAGTCCTGCAGCTATCAGGTCGGCTATGATGACTACGTCGGACATCCTGGATAACACACTTAGTTCCATTAAGGACATTGGTGATCATTTGCTACCGGCTAGTCCTCTGGCCATGGGAGCAGGTCATGTCAATCCCAACAAGGCCCTTAACCCGGGACTCATATATGACGCGACCATAGATGATTACGTAAATCTTCTTTGTGCACTAAACTACACCAAGGAACAAATCCAAACCATCACCAGATCAGCCTCGAACAATTGTTCATCTCCGTCATTAGATCTTAATTACCCTTCGTTCATTGCATTTTTCAATGTCAATGATTCAAAGTCGGGAGTACAAACCACTCAAGAGTTCCGAAGGACGGTGACCAATGTAGGAAAGGGGCAGTCGACATACATTGCAAGCGTGACAGCTGTGAAAGGGTTTGAGGTTGGAGTCGTTCCTAACAAATTGGTTTTCAGAAGGGAAGGGGAGCAGCTGAGCTTTAAGTTGAGTATAAGAGGTCGAAGACTGATGAAAGAGACGGTGGCTTTTGGTTACCTGACTTGGGTGGACACTGAAGGTCAACATGTGGTGAGAAGCCCCATTGTGGCCACAAAACTCAGCTCGGATGTAGTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

761

Amino Acids

81.78

Weight (kDa)

6.26

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 111 8.5e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 568 5.3e-44 Subtilase family
fn3_6 PF17766 647 - 751 9.8e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 349
Acc36I ACCTGC 2 cut(s) 330, 1772
Acc65I GGTACC 1 cut(s) 413
AccB1I GGYRCC 3 cut(s) 413, 736, 782
AccI GTMKAC 1 cut(s) 2051
AccII CGCG 2 cut(s) 336, 1832
AciI CCGC 2 cut(s) 672, 729
AcoI YGGCCR 7 cut(s) 389, 446, 669, 687, 753, 1770, 2253
AcsI RAATTY 1 cut(s) 1577
AcuI CTGAAG 2 cut(s) 294, 2241
AcvI CACGTG 1 cut(s) 751
AcyI GRCGYC 2 cut(s) 737, 927
AfiI CCNNNNNNNGG 9 cut(s) 297, 444, 968, 1074, 1331, 1813, 2023, 2209, 2251
AflIII ACRYGT 1 cut(s) 2230
AgsI TTSAA 7 cut(s) 1110, 1153, 1232, 1394, 1582, 1972, 1986
AhlI ACTAGT 1 cut(s) 1433
AjnI CCWGG 3 cut(s) 295, 1191, 1707
AjuI GAANNNNNNNTTGG 4 cut(s) 596, 628, 1367, 1399
Alw21I GWGCWC 2 cut(s) 1651, 1867
Alw26I GTCTC 5 cut(s) 79, 263, 312, 1408, 2177
Alw44I GTGCAC 2 cut(s) 1647, 1863
AlwNI CAGNNNCTG 1 cut(s) 1567
Ama87I CYCGRG 2 cut(s) 741, 1813
ApaLI GTGCAC 2 cut(s) 1647, 1863
ApeKI GCWGC 7 cut(s) 806, 1010, 1517, 1619, 1631, 1670, 2137
ApoI RAATTY 1 cut(s) 1577
AseI ATTAAT 1 cut(s) 1134
Asp700I GAANNNNTTC 1 cut(s) 1534
Asp718I GGTACC 1 cut(s) 413
AspLEI GCGC 3 cut(s) 739, 877, 1636
AspS9I GGNCC 4 cut(s) 886, 959, 1451, 1804
AsuC2I CCSGG 7 cut(s) 439, 691, 717, 1092, 1500, 1814, 1815
AsuHPI GGTGA 7 cut(s) 143, 607, 1346, 1754, 1891, 2038, 2248
AvaI CYCGRG 2 cut(s) 741, 1813
AvaII GGWCC 3 cut(s) 886, 959, 1451
BaeGI GKGCMC 3 cut(s) 1613, 1651, 1867
BalI TGGCCA 4 cut(s) 448, 755, 1772, 2255
BanI GGYRCC 3 cut(s) 413, 736, 782
BanII GRGCYC 1 cut(s) 748
BbrPI CACGTG 1 cut(s) 751
BbsI GAAGAC 3 cut(s) 922, 1098, 2176
Bbv12I GWGCWC 2 cut(s) 1651, 1867
BbvI GCAGC 7 cut(s) 793, 1022, 1504, 1618, 1631, 1682, 2149
BccI CCATC 7 cut(s) 24, 492, 818, 860, 923, 1462, 1904
BceAI ACGGC 2 cut(s) 421, 684
BciT130I CCWGG 3 cut(s) 297, 1193, 1709
BclI TGATCA 1 cut(s) 1744
BcnI CCSGG 7 cut(s) 439, 691, 717, 1092, 1500, 1814, 1815
BcoDI GTCTC 5 cut(s) 79, 263, 312, 1408, 2177
BcuI ACTAGT 1 cut(s) 1433
BfaI CTAG 4 cut(s) 1014, 1434, 1446, 1761
BfmI CTRYAG 2 cut(s) 174, 1668
BfoI RGCGCY 1 cut(s) 740
BfuAI ACCTGC 2 cut(s) 330, 1772
BglII AGATCT 1 cut(s) 1939
BisI GCNGC 9 cut(s) 672, 730, 807, 1011, 1518, 1620, 1632, 1671, 2138
BlpI GCTNAGC 1 cut(s) 2141
BlsI GCNGC 9 cut(s) 673, 731, 808, 1012, 1519, 1621, 1633, 1672, 2139
Bme18I GGWCC 3 cut(s) 886, 959, 1451
BmeT110I CYCGRG 2 cut(s) 741, 1813
BmgT120I GGNCC 4 cut(s) 886, 959, 1451, 1804
BmiI GGNNCC 4 cut(s) 415, 738, 784, 888
BmrI ACTGGG 1 cut(s) 349
BmsI GCATC 3 cut(s) 704, 1146, 1225
BmuI ACTGGG 1 cut(s) 349
BpiI GAAGAC 3 cut(s) 922, 1098, 2176
BpmI CTGGAG 3 cut(s) 279, 296, 1277
Bpu10I CCTNAGC 1 cut(s) 270
Bpu1102I GCTNAGC 1 cut(s) 2141
BpuEI CTTGAG 3 cut(s) 1229, 1463, 1992
BpuMI CCSGG 7 cut(s) 439, 691, 717, 1092, 1500, 1814, 1815
BsaAI YACGTR 2 cut(s) 751, 1849
BsaHI GRCGYC 2 cut(s) 737, 927
BsaI GGTCTC 2 cut(s) 312, 1408
BsaXI ACNNNNNCTCC 2 cut(s) 1053, 1083
Bsc4I CCNNNNNNNGG 9 cut(s) 297, 444, 968, 1074, 1331, 1813, 2023, 2209, 2251
Bse118I RCCGGY 3 cut(s) 325, 391, 1756
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 2 cut(s) 1959, 2058
BseBI CCWGG 3 cut(s) 297, 1193, 1709
BseGI GGATG 7 cut(s) 108, 844, 1083, 1598, 1651, 1704, 2278
BseLI CCNNNNNNNGG 9 cut(s) 297, 444, 968, 1074, 1331, 1813, 2023, 2209, 2251
BseMI GCAATG 2 cut(s) 1959, 2058
BseMII CTCAG 4 cut(s) 261, 924, 2132, 2278
BseNI ACTGG 1 cut(s) 355
BseSI GKGCMC 3 cut(s) 1613, 1651, 1867
BseX3I CGGCCG 1 cut(s) 669
BseXI GCAGC 7 cut(s) 793, 1022, 1504, 1618, 1631, 1682, 2149
BseYI CCCAGC 1 cut(s) 1622
BsgI GTGCAG 2 cut(s) 1029, 1479
Bsh1236I CGCG 2 cut(s) 336, 1832
Bsh1285I CGRYCG 1 cut(s) 672
BshNI GGYRCC 3 cut(s) 413, 736, 782
BsiEI CGRYCG 1 cut(s) 672
BsiHKAI GWGCWC 2 cut(s) 1651, 1867
BsiHKCI CYCGRG 2 cut(s) 741, 1813
BslFI GGGAC 6 cut(s) 337, 647, 664, 788, 1609, 1830
BslI CCNNNNNNNGG 9 cut(s) 297, 444, 968, 1074, 1331, 1813, 2023, 2209, 2251
BsmAI GTCTC 5 cut(s) 79, 263, 312, 1408, 2177
BsmBI CGTCTC 1 cut(s) 2177
BsmFI GGGAC 6 cut(s) 337, 647, 664, 788, 1609, 1830
Bso31I GGTCTC 2 cut(s) 312, 1408
BsoBI CYCGRG 2 cut(s) 741, 1813
Bsp1286I GDGCHC 4 cut(s) 748, 1613, 1651, 1867
Bsp1407I TGTACA 1 cut(s) 759
Bsp143I GATC 5 cut(s) 148, 1024, 1744, 1904, 1939
Bsp1720I GCTNAGC 1 cut(s) 2141
Bsp19I CCATGG 2 cut(s) 1602, 1773
BspACI CCGC 2 cut(s) 672, 729
BspCNI CTCAG 4 cut(s) 262, 923, 2133, 2277
BspFNI CGCG 2 cut(s) 336, 1832
BspHI TCATGA 1 cut(s) 88
BspLI GGNNCC 4 cut(s) 415, 738, 784, 888
BspMAI CTGCAG 1 cut(s) 1672
BspMI ACCTGC 2 cut(s) 330, 1772
BspQI GCTCTTC 1 cut(s) 541
BspT107I GGYRCC 3 cut(s) 413, 736, 782
BspTNI GGTCTC 2 cut(s) 312, 1408
BsrDI GCAATG 2 cut(s) 1959, 2058
BsrFI RCCGGY 3 cut(s) 325, 391, 1756
BsrGI TGTACA 1 cut(s) 759
BsrI ACTGG 1 cut(s) 355
BssAI RCCGGY 3 cut(s) 325, 391, 1756
BssMI GATC 5 cut(s) 148, 1024, 1744, 1904, 1939
BssNI GRCGYC 2 cut(s) 737, 927
BssT1I CCWWGG 5 cut(s) 81, 992, 1602, 1773, 1878
Bst2UI CCWGG 3 cut(s) 297, 1193, 1709
Bst4CI ACNGT 8 cut(s) 175, 644, 833, 1006, 1228, 1429, 2026, 2188
Bst6I CTCTTC 3 cut(s) 211, 541, 667
BstACI GRCGYC 2 cut(s) 737, 927
BstAPI GCANNNNNTGC 1 cut(s) 1244
BstAUI TGTACA 1 cut(s) 759
BstBAI YACGTR 2 cut(s) 751, 1849
BstC8I GCNNGC 5 cut(s) 727, 819, 977, 1609, 2065
BstDEI CTNAG 6 cut(s) 210, 270, 910, 1721, 2141, 2264
BstDSI CCRYGG 2 cut(s) 1602, 1773
BstEII GGTNACC 2 cut(s) 2026, 2198
BstF5I GGATG 7 cut(s) 108, 844, 1083, 1598, 1651, 1704, 2278
BstFNI CGCG 2 cut(s) 336, 1832
BstH2I RGCGCY 1 cut(s) 740
BstHHI GCGC 3 cut(s) 739, 877, 1636
BstKTI GATC 5 cut(s) 151, 1027, 1747, 1907, 1942
BstMAI GTCTC 5 cut(s) 79, 263, 312, 1408, 2177
BstMBI GATC 5 cut(s) 148, 1024, 1744, 1904, 1939
BstMCI CGRYCG 1 cut(s) 672
BstMWI GCNNNNNNNGC 5 cut(s) 743, 752, 1244, 1628, 1778
BstNI CCWGG 3 cut(s) 297, 1193, 1709
BstNSI RCATGY 3 cut(s) 1143, 1619, 2234
BstPI GGTNACC 2 cut(s) 2026, 2198
BstSFI CTRYAG 2 cut(s) 174, 1668
BstSLI GKGCMC 3 cut(s) 1613, 1651, 1867
BstSNI TACGTA 1 cut(s) 1849
BstUI CGCG 2 cut(s) 336, 1832
BstV1I GCAGC 7 cut(s) 793, 1022, 1504, 1618, 1631, 1682, 2149
BstV2I GAAGAC 3 cut(s) 922, 1098, 2176
BstX2I RGATCY 1 cut(s) 1939
BstXI CCANNNNNNTGG 3 cut(s) 362, 498, 733
BstYI RGATCY 1 cut(s) 1939
BstZI CGGCCG 1 cut(s) 669
BtgI CCRYGG 2 cut(s) 1602, 1773
BtsCI GGATG 7 cut(s) 108, 844, 1083, 1598, 1651, 1704, 2278
BtsI GCAGTG 1 cut(s) 1400
BtsIMutI CAGTG 4 cut(s) 171, 256, 1400, 2217
BveI ACCTGC 2 cut(s) 330, 1772
Cac8I GCNNGC 5 cut(s) 727, 819, 977, 1609, 2065
CaiI CAGNNNCTG 1 cut(s) 1567
CciI TCATGA 1 cut(s) 88
CfoI GCGC 3 cut(s) 739, 877, 1636
Cfr10I RCCGGY 3 cut(s) 325, 391, 1756
Cfr13I GGNCC 4 cut(s) 886, 959, 1451, 1804
Cfr9I CCCGGG 1 cut(s) 1813
CseI GACGC 4 cut(s) 479, 661, 916, 1838
DdeI CTNAG 6 cut(s) 210, 270, 910, 1721, 2141, 2264
DinI GGCGCC 1 cut(s) 738
DpnI GATC 5 cut(s) 150, 1026, 1746, 1906, 1941
DpnII GATC 5 cut(s) 148, 1024, 1744, 1904, 1939
DrdI GACNNNNNNGTC 1 cut(s) 349
DseDI GACNNNNNNGTC 1 cut(s) 349
EaeI YGGCCR 7 cut(s) 389, 446, 669, 687, 753, 1770, 2253
EagI CGGCCG 1 cut(s) 669
Eam1104I CTCTTC 3 cut(s) 211, 541, 667
EarI CTCTTC 3 cut(s) 211, 541, 667
EclXI CGGCCG 1 cut(s) 669
Eco105I TACGTA 1 cut(s) 1849
Eco130I CCWWGG 5 cut(s) 81, 992, 1602, 1773, 1878
Eco147I AGGCCT 1 cut(s) 281
Eco24I GRGCYC 1 cut(s) 748
Eco31I GGTCTC 2 cut(s) 312, 1408
Eco47I GGWCC 3 cut(s) 886, 959, 1451
Eco52I CGGCCG 1 cut(s) 669
Eco57I CTGAAG 2 cut(s) 294, 2241
Eco72I CACGTG 1 cut(s) 751
Eco88I CYCGRG 2 cut(s) 741, 1813
Eco91I GGTNACC 2 cut(s) 2026, 2198
EcoO109I RGGNCCY 3 cut(s) 886, 959, 1804
EcoO65I GGTNACC 2 cut(s) 2026, 2198
EcoRI GAATTC 1 cut(s) 1577
EcoRII CCWGG 3 cut(s) 295, 1191, 1707
EcoT14I CCWWGG 5 cut(s) 81, 992, 1602, 1773, 1878
EcoT22I ATGCAT 1 cut(s) 1141
EcoT38I GRGCYC 1 cut(s) 748
EgeI GGCGCC 1 cut(s) 738
EheI GGCGCC 1 cut(s) 738
ErhI CCWWGG 5 cut(s) 81, 992, 1602, 1773, 1878
Esp3I CGTCTC 1 cut(s) 2177
FalI AAGNNNNNCTT 2 cut(s) 914, 946
FaqI GGGAC 6 cut(s) 337, 647, 664, 788, 1609, 1830
FbaI TGATCA 1 cut(s) 1744
FblI GTMKAC 1 cut(s) 2051
Fnu4HI GCNGC 9 cut(s) 672, 730, 807, 1011, 1518, 1620, 1632, 1671, 2138
FokI GGATG 6 cut(s) 95, 851, 1090, 1585, 1638, 1691
FriOI GRGCYC 1 cut(s) 748
Fsp4HI GCNGC 9 cut(s) 672, 730, 807, 1011, 1518, 1620, 1632, 1671, 2138
FspBI CTAG 4 cut(s) 1014, 1434, 1446, 1761
GlaI GCGC 3 cut(s) 738, 876, 1635
GluI GCNGC 9 cut(s) 672, 730, 807, 1011, 1518, 1620, 1632, 1671, 2138
GsaI CCCAGC 1 cut(s) 1626
GsuI CTGGAG 3 cut(s) 279, 296, 1277
HaeII RGCGCY 1 cut(s) 740
HgaI GACGC 4 cut(s) 479, 661, 916, 1838
HhaI GCGC 3 cut(s) 739, 877, 1636
Hin1I GRCGYC 2 cut(s) 737, 927
Hin6I GCGC 3 cut(s) 737, 875, 1634
HinP1I GCGC 3 cut(s) 737, 875, 1634
HincII GTYRAC 2 cut(s) 2052, 2228
HindII GTYRAC 2 cut(s) 2052, 2228
HindIII AAGCTT 2 cut(s) 460, 1381
HphI GGTGA 7 cut(s) 143, 607, 1346, 1754, 1891, 2038, 2248
Hpy188I TCNGA 7 cut(s) 484, 796, 860, 1702, 2018, 2123, 2272
Hpy99I CGWCG 4 cut(s) 473, 644, 1429, 1702
HpyAV CCTTC 8 cut(s) 916, 950, 1325, 1962, 2013, 2118, 2123, 2216
HpyCH4III ACNGT 8 cut(s) 175, 644, 833, 1006, 1228, 1429, 2026, 2188
HpyCH4IV ACGT 3 cut(s) 750, 1697, 1848
HpyF10VI GCNNNNNNNGC 5 cut(s) 743, 752, 1244, 1628, 1778
HpyF3I CTNAG 6 cut(s) 210, 270, 910, 1721, 2141, 2264
HpySE526I ACGT 3 cut(s) 750, 1697, 1848
Hsp92I GRCGYC 2 cut(s) 737, 927
HspAI GCGC 3 cut(s) 737, 875, 1634
KasI GGCGCC 1 cut(s) 736
KpnI GGTACC 1 cut(s) 417
Ksp22I TGATCA 1 cut(s) 1744
Kzo9I GATC 5 cut(s) 148, 1024, 1744, 1904, 1939
LguI GCTCTTC 1 cut(s) 541
LmnI GCTCC 4 cut(s) 383, 718, 1778, 2134
Lsp1109I GCAGC 7 cut(s) 793, 1022, 1504, 1618, 1631, 1682, 2149
LweI GCATC 3 cut(s) 704, 1146, 1225
MaeI CTAG 4 cut(s) 1014, 1434, 1446, 1761
MaeII ACGT 3 cut(s) 750, 1697, 1848
MalI GATC 5 cut(s) 150, 1026, 1746, 1906, 1941
MboI GATC 5 cut(s) 148, 1024, 1744, 1904, 1939
MfeI CAATTG 2 cut(s) 1122, 1918
MflI RGATCY 1 cut(s) 1939
MhlI GDGCHC 4 cut(s) 748, 1613, 1651, 1867
MlsI TGGCCA 4 cut(s) 448, 755, 1772, 2255
MluNI TGGCCA 4 cut(s) 448, 755, 1772, 2255
Mly113I GGCGCC 1 cut(s) 737
MlyI GAGTC 5 cut(s) 1075, 1330, 1672, 1812, 2106
MmeI TCCRAC 3 cut(s) 120, 1680, 2074
Mox20I TGGCCA 4 cut(s) 448, 755, 1772, 2255
Mph1103I ATGCAT 1 cut(s) 1141
MroXI GAANNNNTTC 1 cut(s) 1534
MscI TGGCCA 4 cut(s) 448, 755, 1772, 2255
MslI CAYNNNNRTG 4 cut(s) 113, 496, 1076, 1538
Msp20I TGGCCA 4 cut(s) 448, 755, 1772, 2255
MspA1I CMGCKG 5 cut(s) 674, 729, 1622, 2075, 2140
MunI CAATTG 2 cut(s) 1122, 1918
MvaI CCWGG 3 cut(s) 297, 1193, 1709
MvnI CGCG 2 cut(s) 336, 1832
MwoI GCNNNNNNNGC 5 cut(s) 743, 752, 1244, 1628, 1778
NarI GGCGCC 1 cut(s) 737
NciI CCSGG 7 cut(s) 439, 691, 717, 1092, 1500, 1814, 1815
NcoI CCATGG 2 cut(s) 1602, 1773
NdeII GATC 5 cut(s) 148, 1024, 1744, 1904, 1939
NlaIV GGNNCC 4 cut(s) 415, 738, 784, 888
NmuCI GTSAC 7 cut(s) 49, 607, 1028, 1069, 1142, 2026, 2068
NsiI ATGCAT 1 cut(s) 1141
NspI RCATGY 3 cut(s) 1143, 1619, 2234
PagI TCATGA 1 cut(s) 88
PceI AGGCCT 1 cut(s) 281
PciI ACATGT 1 cut(s) 2230
PciSI GCTCTTC 1 cut(s) 541
PcsI WCGNNNNNNNCGW 3 cut(s) 347, 924, 1544
PdmI GAANNNNTTC 1 cut(s) 1534
PfeI GAWTC 7 cut(s) 92, 119, 179, 585, 987, 1064, 1982
PflFI GACNNNGTC 1 cut(s) 1696
PfoI TCCNGGA 2 cut(s) 715, 1707
PkrI GCNGC 9 cut(s) 673, 731, 808, 1012, 1519, 1621, 1633, 1672, 2139
PleI GAGTC 5 cut(s) 1075, 1329, 1671, 1812, 2105
PluTI GGCGCC 1 cut(s) 740
PmaCI CACGTG 1 cut(s) 751
PmlI CACGTG 1 cut(s) 751
PpsI GAGTC 5 cut(s) 1075, 1329, 1671, 1812, 2105
Ppu21I YACGTR 2 cut(s) 751, 1849
PpuMI RGGWCCY 2 cut(s) 886, 959
PscI ACATGT 1 cut(s) 2230
PshBI ATTAAT 1 cut(s) 1134
Psp5II RGGWCCY 2 cut(s) 886, 959
Psp6I CCWGG 3 cut(s) 295, 1191, 1707
PspCI CACGTG 1 cut(s) 751
PspEI GGTNACC 2 cut(s) 2026, 2198
PspFI CCCAGC 1 cut(s) 1622
PspGI CCWGG 3 cut(s) 295, 1191, 1707
PspN4I GGNNCC 4 cut(s) 415, 738, 784, 888
PspPI GGNCC 4 cut(s) 886, 959, 1451, 1804
PspPPI RGGWCCY 2 cut(s) 886, 959
PstI CTGCAG 1 cut(s) 1672
PstNI CAGNNNCTG 1 cut(s) 1567
PsuI RGATCY 1 cut(s) 1939
PsyI GACNNNGTC 1 cut(s) 1696
PvuII CAGCTG 3 cut(s) 1622, 2075, 2140
RseI CAYNNNNRTG 4 cut(s) 113, 496, 1076, 1538
SalI GTCGAC 1 cut(s) 2050
SapI GCTCTTC 1 cut(s) 541
SatI GCNGC 9 cut(s) 672, 730, 807, 1011, 1518, 1620, 1632, 1671, 2138
Sau3AI GATC 5 cut(s) 148, 1024, 1744, 1904, 1939
Sau96I GGNCC 4 cut(s) 886, 959, 1451, 1804
SchI GAGTC 5 cut(s) 1075, 1330, 1672, 1812, 2106
SduI GDGCHC 4 cut(s) 748, 1613, 1651, 1867
SfaNI GCATC 3 cut(s) 704, 1146, 1225
SfcI CTRYAG 2 cut(s) 174, 1668
SfoI GGCGCC 1 cut(s) 738
SinI GGWCC 3 cut(s) 886, 959, 1451
SmaI CCCGGG 1 cut(s) 1815
SmiMI CAYNNNNRTG 4 cut(s) 113, 496, 1076, 1538
SmlI CTYRAG 3 cut(s) 1208, 1478, 2007
SmoI CTYRAG 3 cut(s) 1208, 1478, 2007
SnaBI TACGTA 1 cut(s) 1849
SpeI ACTAGT 1 cut(s) 1433
SseBI AGGCCT 1 cut(s) 281
SsiI CCGC 2 cut(s) 672, 729
SspDI GGCGCC 1 cut(s) 736
SspMI CTAG 4 cut(s) 1014, 1434, 1446, 1761
StuI AGGCCT 1 cut(s) 281
StyI CCWWGG 5 cut(s) 81, 992, 1602, 1773, 1878
TaaI ACNGT 8 cut(s) 175, 644, 833, 1006, 1228, 1429, 2026, 2188
TaiI ACGT 3 cut(s) 753, 1700, 1851
TaqI TCGA 6 cut(s) 813, 918, 1528, 1913, 2051, 2167
TatI WGTACW 4 cut(s) 30, 184, 759, 1996
TauI GCSGC 2 cut(s) 674, 732
TfiI GAWTC 7 cut(s) 92, 119, 179, 585, 987, 1064, 1982
TscAI CASTG 4 cut(s) 178, 256, 1407, 2224
TseFI GTSAC 7 cut(s) 49, 607, 1028, 1069, 1142, 2026, 2068
TseI GCWGC 7 cut(s) 806, 1010, 1517, 1619, 1631, 1670, 2137
Tsp45I GTSAC 7 cut(s) 49, 607, 1028, 1069, 1142, 2026, 2068
TspGWI ACGGA 2 cut(s) 1057, 1920
TspMI CCCGGG 1 cut(s) 1813
TspRI CASTG 4 cut(s) 178, 256, 1407, 2224
Tth111I GACNNNGTC 1 cut(s) 1696
VneI GTGCAC 2 cut(s) 1647, 1863
VpaK11BI GGWCC 3 cut(s) 886, 959, 1451
VspI ATTAAT 1 cut(s) 1134
XapI RAATTY 1 cut(s) 1577
XbaI TCTAGA 1 cut(s) 1445
XceI RCATGY 3 cut(s) 1143, 1619, 2234
XcmI CCANNNNNNNNNTGG 1 cut(s) 424
XmaI CCCGGG 1 cut(s) 1813
XmiI GTMKAC 1 cut(s) 2051
XmnI GAANNNNTTC 1 cut(s) 1534
XspI CTAG 4 cut(s) 1014, 1434, 1446, 1761
Zsp2I ATGCAT 1 cut(s) 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.