Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001268

Physical Location & Seq

Genomic Coordinates Forward (+)

5494390 .. 5496690

Gene Structure 2301 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1080600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2301 bp

ATGACTAGTTACGATCATGTTTCCTTGTATTTTTGGCTTTCCATTGCCACCATCTCACACCTTGCAATTGTCTCAACCTTTGCTCAATCCCAAAACTATATCATCCACATGGATTCATCAGTGATGCCTAAAGCCTTTTTTGATCACCATTCATGGTATTTGGCAACGTTAGATTCCACATTAGGCAAGTTTACCCCTAGCACCACTGCGACCTCCTCCTCCTCCACCCTGTCTTCCAGACTCATTTATAGTTATACTCATGCCATGAATGGTTTCAGTGCAAGTCTCACTGCTTCCGAGCTTGAGGCCTTAAAAAGCTCTCCAGGCTACATTTCTTCAGTGAAAGACTTGCCAGTTAAAAAAGACACAACTCACACCTCCCAGTTTCTTGGCCTCAACTCCAAATCCGGAGTGTGGCCGGTTGCGAATTACGGAAAAGATGTGATCATTGGTTTGGTGGACACCGGCGTTTGGCCAGAAAGTGAAAGCTTTAACGAAGATGGAATGTCAGAAATCCCACCAAGATGGAAAGGAGAATGTGAAAGTGGCACCCAATTCAACTCCTCATTGTGCAACAAAAAGCTTATTGGGGCAAAGTTCTTTAACAAAGGGCTCGTTGCCCAGAATCCCAATTTGACCATTGAGGTGAATTCAACGAGGGACACTGATGGGCATGGGACTCACACCTCGTCTACGGCCGCAGGAAATTATGTGGCCGGGGCATCATTTTTTGGTTACGCCCCAGGAACTGCCAGTGGCATGGCTCCTAGAGCGCATGTGGCCATGTACAAGGCTCTTTGGGGTGTGGCGGGAGCATTGTCGTCCGATATAATTGCTGCCATTGAGCAAGCAATTATAGACGGTGTGGACGTTTTGTCCTTGTCATTTGGGTTTGATGGTGTTCATTTGTATGAGGATCCTCTTGCCATAGCAACATTTTCTGCCTTGGAGAGAGGTGTTTTTGTGTCCACATCGGCAGGAAATGAGGGTCCTTATTTTGGTACCTTGCACAATGGTATCCCTTGGGTTCTCACGGTTGCAGCTGGTACAATTGATCGGGAATTCAAGGGAACTGCGCATCATGGAAATGGAAACTCCGTCACGGGGTCCACGCTCTATCCCGGAAATTCATCTTCAACCCAATTTCCAGTTGTTTTCTTGGATGCGTGTAACAATAGGAAAACATTGAAGCAGTTGGGGAAGAAAATCGTCGTGTGCCAGGACAGAAATGATACGTTGGACTATCAAGTTTATAATGTCAGCAGAGCAAATGTTGCCGGAGGCATCTTCATTACCAGCAACACAGATTTGGAGTTATTCATTCAAAGCATTTTTCCCGCAATCTTTCTGAGTCCAAAGGAAGGTGAAGTGATCAAAGATTACATCAACAGCAACTCACGGCCAAAAGCAAGCTTGGAGTTTCAAAAGACACTTCTTGGGGCCAAACCAGCACCAAGTGTTACCAGCTACTCGTCTAGAGGGCCTTCCTTTAGCTTCCCCTGGACCTTGAAGCCAGACATTCTGGCTCCAGGGTCGTTAGTCTTGGCTGCATGGCCTCCAAATATCTTCGCGGCCGTGGTGGGAAAGAAAGACTTGTTCAGTAATTTTAATCTACTGTCAGGGACATCCATGTCGTGCCCCCATGTAGCTGGAATAGCGGCGCTGCTGAAAGGTGCACACCCAGAATGGAGCCCTGCTGCTATTAGATCCGCCATGATGACGACATCAGACATATTGGATAACACGGGTAGCCCCATAAAAGACATCGGTGATGCTTACCAGCCAGCTAGTCCTCTAGCCATCGGAGCCGGGCATGTCAACCCTAACAAGGCCCTAGACCCCGGGCTCATCTACGATGCGAAAATTGAAGACTACGTTAACCTTCTTTGTGCACTCAACTACACCAATAAGCAAATCCAAACCATCACTAAATATGCCTCGAACAATTGTTCAACTCCTTCTTTAGATCTTAACTACCCTTCTTTCATTGCATTCTTCAACTCAAACAAGAAGCACTCGGACGTACAAACGACTCATGAATTCCGGAGGACGGTGACCAACCTAGGAAAGGGGCCATCGACCTACGTTGCCAGCTTGACACCGTTGAAAGGGTTTGTGGTTGGAGTGGTTCCGGAGAAGTTAGAGTTCACGGAGGTGGGTGAGAAGCTGAGCTTTGTGATGAGTATAAAAGGTCCAAGAGTAATGAAAGAGACAGTGGTTTTTGGTTCTCTGAGTTGGGTGGAGAGTGGAGGTGAACATGTGGTGAGAAGCCCCGTCGTGGCTTCAAGCCTCATGAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

767

Amino Acids

82.16

Weight (kDa)

6.47

Isoelectric Point (pI)

33.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	32 - 120	1.4e-15	Peptidase inhibitor I9
Peptidase_S8	PF00082	145 - 580	1.5e-41	Subtilase family
fn3_6	PF17766	656 - 760	1.8e-24	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001268)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67090
fragaria_vesca	FvH4_1g16180 FvH4_2g34170 FvH4_2g34170 FvH4_2g34230 FvH4_6g01060 FvH4_6g01061
malus_domestica	MD04G1236800.v1.1 MD04G1236900.v1.1 MD08G1098100.v1.1 MD15G1080600.v1.1 MD15G1285300.v1.1
prunus_persica	Prupe.1G433300_v2.0.a1 Prupe.6G168400_v2.0.a1 Prupe.6G356800_v2.0.a1 Prupe.7G131900_v2.0.a1
pyrus_communis	pycom04g21060 pycom04g21070 pycom08g07990 pycom12g23460 pycom15g07490 pycom15g24800
rosa_chinensis	RchiOBHm_Chr2g0105861 RchiOBHm_Chr2g0105881 RchiOBHm_Chr3g0448571 RchiOBHm_Chr6g0308191
rosa_laevigata	RLG00000010660 RLG00000017466 RLG00000017467 RLG00000025886 RLG00000025888 RLG00000025889 RLG00000025890
rosa_multiflora	Rmu_sc0003116.1_g000043 Rmu_sc0004367.1_g000007 Rmu_sc0004367.1_g000008 Rmu_sc0005706.1_g000016 Rmu_sc0010389.1_g000023
rosa_roxburghii	Rroxscaffold_2G00137780 Rroxscaffold_6G00426820 Rroxscaffold_6G00426860 Rroxscaffold_7G00160370 Rroxscaffold_7G00160460
rosa_rugosa	Rorug02G0131300 Rorug02G0131500 Rorug02G0613500 Rorug02G0613600 Rorug06G0364600
rosa_samantha	Rh2CG186700 Rh3DG013500 Rh6DG475800
rosa_wichuraiana	Rw2G014280 Rw2G014290 Rw3G000970 Rw6G041320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1254
AasI	GACNNNNNNGTC	1 cut(s)	1630
Acc16I	TGCGCA	1 cut(s)	1077
Acc65I	GGTACC	1 cut(s)	1001
AccB1I	GGYRCC	2 cut(s)	548, 1001
AccI	GTMKAC	1 cut(s)	692
AccII	CGCG	1 cut(s)	1571
AccIII	TCCGGA	3 cut(s)	407, 2043, 2131
AciI	CCGC	6 cut(s)	699, 809, 1338, 1571, 1658, 1710
AclI	AACGTT	1 cut(s)	167
AclWI	GGATC	3 cut(s)	911, 924, 1701
AcoI	YGGCCR	7 cut(s)	416, 473, 696, 714, 780, 1400, 1572
AcsI	RAATTY	4 cut(s)	649, 1061, 1126, 2039
AcuI	CTGAAG	1 cut(s)	321
AdeI	CACNNNGTG	1 cut(s)	1457
AfaI	GTAC	4 cut(s)	788, 1003, 1048, 2025
AflIII	ACRYGT	1 cut(s)	2257
AhdI	GACNNNNNGTC	1 cut(s)	874
AhlI	ACTAGT	1 cut(s)	5
AjnI	CCWGG	5 cut(s)	322, 742, 1218, 1499, 1528
AleI	CACNNNNGTG	1 cut(s)	2153
Alw21I	GWGCWC	2 cut(s)	1678, 1894
Alw26I	GTCTC	3 cut(s)	76, 290, 2204
Alw44I	GTGCAC	2 cut(s)	1674, 1890
AlwI	GGATC	3 cut(s)	911, 924, 1701
AlwNI	CAGNNNCTG	1 cut(s)	749
Ama87I	CYCGRG	1 cut(s)	1841
Aor13HI	TCCGGA	3 cut(s)	407, 2043, 2131
ApaLI	GTGCAC	2 cut(s)	1674, 1890
ApeKI	GCWGC	5 cut(s)	836, 1040, 1547, 1663, 1697
ApoI	RAATTY	4 cut(s)	649, 1061, 1126, 2039
Asp700I	GAANNNNTTC	1 cut(s)	272
Asp718I	GGTACC	1 cut(s)	1001
AspA2I	CCTAGG	1 cut(s)	2062
AspLEI	GCGC	3 cut(s)	775, 1078, 1663
AspS9I	GGNCC	8 cut(s)	989, 1107, 1440, 1481, 1503, 1831, 2072, 2192
AsuC2I	CCSGG	5 cut(s)	718, 1122, 1810, 1842, 1843
AsuHPI	GGTGA	8 cut(s)	137, 658, 1376, 1781, 2065, 2171, 2264, 2275
AvaI	CYCGRG	1 cut(s)	1841
AvaII	GGWCC	4 cut(s)	989, 1107, 1503, 2192
AvrII	CCTAGG	1 cut(s)	2062
BaeGI	GKGCMC	3 cut(s)	1640, 1678, 1894
BaeI	ACNNNNGTAYC	2 cut(s)	1000, 1033
BalI	TGGCCA	2 cut(s)	475, 782
BamHI	GGATCC	1 cut(s)	916
BanI	GGYRCC	2 cut(s)	548, 1001
BanII	GRGCYC	3 cut(s)	615, 1694, 1848
BbsI	GAAGAC	2 cut(s)	225, 1875
Bbv12I	GWGCWC	2 cut(s)	1678, 1894
BbvI	GCAGC	5 cut(s)	823, 1052, 1534, 1650, 1684
BccI	CCATC	8 cut(s)	59, 494, 519, 662, 890, 1808, 1931, 2083
BceAI	ACGGC	3 cut(s)	711, 1415, 1559
BcgI	CGANNNNNNTGC	2 cut(s)	815, 849
BciT130I	CCWGG	5 cut(s)	324, 744, 1220, 1501, 1530
BciVI	GTATCC	1 cut(s)	1028
BclI	TGATCA	3 cut(s)	142, 444, 1371
BcnI	CCSGG	5 cut(s)	718, 1122, 1810, 1842, 1843
BcoDI	GTCTC	3 cut(s)	76, 290, 2204
BcuI	ACTAGT	1 cut(s)	5
BfaI	CTAG	8 cut(s)	6, 198, 768, 1476, 1788, 1796, 1835, 2063
BfoI	RGCGCY	1 cut(s)	1664
BfuI	GTATCC	1 cut(s)	1028
BglII	AGATCT	1 cut(s)	1966
BisI	GCNGC	8 cut(s)	699, 837, 1041, 1548, 1572, 1659, 1664, 1698
BlnI	CCTAGG	1 cut(s)	2062
BlpI	GCTNAGC	1 cut(s)	2168
BlsI	GCNGC	8 cut(s)	700, 838, 1042, 1549, 1573, 1660, 1665, 1699
Bme18I	GGWCC	4 cut(s)	989, 1107, 1503, 2192
BmeRI	GACNNNNNGTC	1 cut(s)	874
BmeT110I	CYCGRG	1 cut(s)	1841
BmgT120I	GGNCC	8 cut(s)	989, 1107, 1440, 1481, 1503, 1831, 2072, 2192
BmrI	ACTGGG	1 cut(s)	376
BmsI	GCATC	7 cut(s)	114, 731, 1087, 1153, 1293, 1762, 1846
BmuI	ACTGGG	1 cut(s)	376
BpiI	GAAGAC	2 cut(s)	225, 1875
BpmI	CTGGAG	2 cut(s)	306, 1512
Bpu1102I	GCTNAGC	1 cut(s)	2168
BpuEI	CTTGAG	1 cut(s)	323
BpuMI	CCSGG	5 cut(s)	718, 1122, 1810, 1842, 1843
BsaWI	WCCGGW	3 cut(s)	407, 2043, 2131
Bse118I	RCCGGY	2 cut(s)	418, 464
Bse1I	ACTGG	4 cut(s)	353, 382, 753, 1148
Bse3DI	GCAATG	2 cut(s)	42, 1986
BseAI	TCCGGA	3 cut(s)	407, 2043, 2131
BseBI	CCWGG	5 cut(s)	324, 744, 1220, 1501, 1530
BseGI	GGATG	3 cut(s)	102, 1168, 1625
BseMI	GCAATG	2 cut(s)	42, 1986
BseMII	CTCAG	3 cut(s)	1340, 2159, 2222
BseNI	ACTGG	4 cut(s)	353, 382, 753, 1148
BseRI	GAGGAG	4 cut(s)	205, 208, 211, 553
BseSI	GKGCMC	3 cut(s)	1640, 1678, 1894
BseX3I	CGGCCG	2 cut(s)	696, 1572
BseXI	GCAGC	5 cut(s)	823, 1052, 1534, 1650, 1684
Bsh1236I	CGCG	1 cut(s)	1571
Bsh1285I	CGRYCG	2 cut(s)	699, 1575
BshNI	GGYRCC	2 cut(s)	548, 1001
BsiEI	CGRYCG	2 cut(s)	699, 1575
BsiHKAI	GWGCWC	2 cut(s)	1678, 1894
BsiHKCI	CYCGRG	1 cut(s)	1841
BslFI	GGGAC	3 cut(s)	674, 691, 1636
BsmAI	GTCTC	3 cut(s)	76, 290, 2204
BsmFI	GGGAC	3 cut(s)	674, 691, 1636
BsmI	GAATGC	1 cut(s)	1991
BsoBI	CYCGRG	1 cut(s)	1841
Bsp1286I	GDGCHC	6 cut(s)	615, 1640, 1678, 1694, 1848, 1894
Bsp13I	TCCGGA	3 cut(s)	407, 2043, 2131
Bsp1407I	TGTACA	1 cut(s)	786
Bsp143I	GATC	8 cut(s)	13, 142, 444, 916, 1054, 1371, 1706, 1966
Bsp1720I	GCTNAGC	1 cut(s)	2168
BspACI	CCGC	6 cut(s)	699, 809, 1338, 1571, 1658, 1710
BspCNI	CTCAG	3 cut(s)	1341, 2160, 2223
BspEI	TCCGGA	3 cut(s)	407, 2043, 2131
BspFNI	CGCG	1 cut(s)	1571
BspHI	TCATGA	2 cut(s)	2035, 2292
BspPI	GGATC	3 cut(s)	911, 924, 1701
BspT107I	GGYRCC	2 cut(s)	548, 1001
BsrDI	GCAATG	2 cut(s)	42, 1986
BsrFI	RCCGGY	2 cut(s)	418, 464
BsrGI	TGTACA	1 cut(s)	786
BsrI	ACTGG	4 cut(s)	353, 382, 753, 1148
BssAI	RCCGGY	2 cut(s)	418, 464
BssMI	GATC	8 cut(s)	13, 142, 444, 916, 1054, 1371, 1706, 1966
BssT1I	CCWWGG	3 cut(s)	945, 1022, 2062
Bst2UI	CCWGG	5 cut(s)	324, 744, 1220, 1501, 1530
Bst4CI	ACNGT	6 cut(s)	863, 1036, 1617, 2053, 2103, 2215
BstAPI	GCANNNNNTGC	1 cut(s)	1274
BstAUI	TGTACA	1 cut(s)	786
BstC8I	GCNNGC	4 cut(s)	849, 1411, 1785, 2092
BstDEI	CTNAG	3 cut(s)	1349, 2168, 2231
BstDSI	CCRYGG	1 cut(s)	1575
BstEII	GGTNACC	1 cut(s)	2053
BstENI	CCTNNNNNAGG	1 cut(s)	2066
BstF5I	GGATG	3 cut(s)	102, 1168, 1625
BstFNI	CGCG	1 cut(s)	1571
BstH2I	RGCGCY	1 cut(s)	1664
BstHHI	GCGC	3 cut(s)	775, 1078, 1663
BstKTI	GATC	8 cut(s)	16, 145, 447, 919, 1057, 1374, 1709, 1969
BstMAI	GTCTC	3 cut(s)	76, 290, 2204
BstMBI	GATC	8 cut(s)	13, 142, 444, 916, 1054, 1371, 1706, 1966
BstMCI	CGRYCG	2 cut(s)	699, 1575
BstMWI	GCNNNNNNNGC	8 cut(s)	324, 770, 779, 1274, 1448, 1655, 1805, 2295
BstNI	CCWGG	5 cut(s)	324, 744, 1220, 1501, 1530
BstNSI	RCATGY	3 cut(s)	779, 1817, 2261
BstPI	GGTNACC	1 cut(s)	2053
BstSLI	GKGCMC	3 cut(s)	1640, 1678, 1894
BstUI	CGCG	1 cut(s)	1571
BstV1I	GCAGC	5 cut(s)	823, 1052, 1534, 1650, 1684
BstV2I	GAAGAC	2 cut(s)	225, 1875
BstX2I	RGATCY	3 cut(s)	916, 1706, 1966
BstXI	CCANNNNNNTGG	4 cut(s)	389, 525, 760, 1649
BstYI	RGATCY	3 cut(s)	916, 1706, 1966
BstZI	CGGCCG	2 cut(s)	696, 1572
BsuI	GTATCC	1 cut(s)	1028
BtgI	CCRYGG	1 cut(s)	1575
BtsCI	GGATG	3 cut(s)	102, 1168, 1625
BtsI	GCAGTG	2 cut(s)	204, 288
BtsIMutI	CAGTG	8 cut(s)	126, 204, 283, 288, 345, 663, 760, 2220
Cac8I	GCNNGC	4 cut(s)	849, 1411, 1785, 2092
CaiI	CAGNNNCTG	1 cut(s)	749
CciI	TCATGA	2 cut(s)	2035, 2292
CfoI	GCGC	3 cut(s)	775, 1078, 1663
Cfr10I	RCCGGY	2 cut(s)	418, 464
Cfr13I	GGNCC	8 cut(s)	989, 1107, 1440, 1481, 1503, 1831, 2072, 2192
Cfr9I	CCCGGG	1 cut(s)	1841
Csp6I	GTAC	4 cut(s)	787, 1002, 1047, 2024
CviQI	GTAC	4 cut(s)	787, 1002, 1047, 2024
DdeI	CTNAG	3 cut(s)	1349, 2168, 2231
DpnI	GATC	8 cut(s)	15, 144, 446, 918, 1056, 1373, 1708, 1968
DpnII	GATC	8 cut(s)	13, 142, 444, 916, 1054, 1371, 1706, 1966
DraIII	CACNNNGTG	1 cut(s)	1457
DrdI	GACNNNNNNGTC	1 cut(s)	1630
DriI	GACNNNNNGTC	1 cut(s)	874
DseDI	GACNNNNNNGTC	1 cut(s)	1630
EaeI	YGGCCR	7 cut(s)	416, 473, 696, 714, 780, 1400, 1572
EagI	CGGCCG	2 cut(s)	696, 1572
Eam1105I	GACNNNNNGTC	1 cut(s)	874
EciI	GGCGGA	1 cut(s)	1699
EclXI	CGGCCG	2 cut(s)	696, 1572
Eco130I	CCWWGG	3 cut(s)	945, 1022, 2062
Eco147I	AGGCCT	1 cut(s)	308
Eco24I	GRGCYC	3 cut(s)	615, 1694, 1848
Eco47I	GGWCC	4 cut(s)	989, 1107, 1503, 2192
Eco52I	CGGCCG	2 cut(s)	696, 1572
Eco57I	CTGAAG	1 cut(s)	321
Eco88I	CYCGRG	1 cut(s)	1841
Eco91I	GGTNACC	1 cut(s)	2053
EcoNI	CCTNNNNNAGG	1 cut(s)	2066
EcoO109I	RGGNCCY	3 cut(s)	989, 1481, 1831
EcoO65I	GGTNACC	1 cut(s)	2053
EcoRI	GAATTC	3 cut(s)	649, 1061, 2039
EcoRII	CCWGG	5 cut(s)	322, 742, 1218, 1499, 1528
EcoT14I	CCWWGG	3 cut(s)	945, 1022, 2062
EcoT38I	GRGCYC	3 cut(s)	615, 1694, 1848
ErhI	CCWWGG	3 cut(s)	945, 1022, 2062
FalI	AAGNNNNNCTT	4 cut(s)	1577, 1609, 2156, 2188
FaqI	GGGAC	3 cut(s)	674, 691, 1636
FauI	CCCGC	2 cut(s)	802, 1345
FbaI	TGATCA	3 cut(s)	142, 444, 1371
FblI	GTMKAC	1 cut(s)	692
Fnu4HI	GCNGC	8 cut(s)	699, 837, 1041, 1548, 1572, 1659, 1664, 1698
FokI	GGATG	3 cut(s)	89, 1175, 1612
FriOI	GRGCYC	3 cut(s)	615, 1694, 1848
Fsp4HI	GCNGC	8 cut(s)	699, 837, 1041, 1548, 1572, 1659, 1664, 1698
FspBI	CTAG	8 cut(s)	6, 198, 768, 1476, 1788, 1796, 1835, 2063
FspI	TGCGCA	1 cut(s)	1077
GlaI	GCGC	3 cut(s)	774, 1077, 1662
GluI	GCNGC	8 cut(s)	699, 837, 1041, 1548, 1572, 1659, 1664, 1698
GsuI	CTGGAG	2 cut(s)	306, 1512
HaeII	RGCGCY	1 cut(s)	1664
HhaI	GCGC	3 cut(s)	775, 1078, 1663
Hin6I	GCGC	3 cut(s)	773, 1076, 1661
HinP1I	GCGC	3 cut(s)	773, 1076, 1661
HincII	GTYRAC	2 cut(s)	1819, 1879
HindII	GTYRAC	2 cut(s)	1819, 1879
HindIII	AAGCTT	3 cut(s)	487, 581, 1411
HinfI	GANTC	7 cut(s)	113, 173, 240, 625, 679, 1351, 2032
HpaI	GTTAAC	1 cut(s)	1879
HphI	GGTGA	8 cut(s)	137, 658, 1376, 1781, 2065, 2171, 2264, 2275
Hpy188I	TCNGA	8 cut(s)	298, 511, 826, 1350, 1729, 1805, 2020, 2232
Hpy188III	TCNNGA	8 cut(s)	237, 408, 1058, 1476, 2036, 2044, 2132, 2293
Hpy99I	CGWCG	2 cut(s)	1214, 2279
HpyAV	CCTTC	5 cut(s)	1355, 1494, 1892, 1968, 1989
HpyCH4III	ACNGT	6 cut(s)	863, 1036, 1617, 2053, 2103, 2215
HpyCH4IV	ACGT	6 cut(s)	167, 870, 1235, 1875, 2022, 2085
HpyCH4V	TGCA	9 cut(s)	65, 281, 573, 1009, 1040, 1550, 1676, 1892, 1991
HpyF10VI	GCNNNNNNNGC	8 cut(s)	324, 770, 779, 1274, 1448, 1655, 1805, 2295
HpyF3I	CTNAG	3 cut(s)	1349, 2168, 2231
HpySE526I	ACGT	6 cut(s)	167, 870, 1235, 1875, 2022, 2085
HspAI	GCGC	3 cut(s)	773, 1076, 1661
Kpn2I	TCCGGA	3 cut(s)	407, 2043, 2131
KpnI	GGTACC	1 cut(s)	1005
Ksp22I	TGATCA	3 cut(s)	142, 444, 1371
KspAI	GTTAAC	1 cut(s)	1879
Kzo9I	GATC	8 cut(s)	13, 142, 444, 916, 1054, 1371, 1706, 1966
LmnI	GCTCC	5 cut(s)	769, 812, 1531, 1689, 1805
Lsp1109I	GCAGC	5 cut(s)	823, 1052, 1534, 1650, 1684
LweI	GCATC	7 cut(s)	114, 731, 1087, 1153, 1293, 1762, 1846
MaeI	CTAG	8 cut(s)	6, 198, 768, 1476, 1788, 1796, 1835, 2063
MaeII	ACGT	6 cut(s)	167, 870, 1235, 1875, 2022, 2085
MaeIII	GTNAC	6 cut(s)	8, 734, 1099, 1169, 1459, 2053
MalI	GATC	8 cut(s)	15, 144, 446, 918, 1056, 1373, 1708, 1968
MboI	GATC	8 cut(s)	13, 142, 444, 916, 1054, 1371, 1706, 1966
MboII	GAAGA	9 cut(s)	225, 327, 509, 1125, 1213, 1279, 1558, 1880, 1987
MfeI	CAATTG	3 cut(s)	66, 1050, 1945
MflI	RGATCY	3 cut(s)	916, 1706, 1966
MhlI	GDGCHC	6 cut(s)	615, 1640, 1678, 1694, 1848, 1894
MlsI	TGGCCA	2 cut(s)	475, 782
MluNI	TGGCCA	2 cut(s)	475, 782
MlyI	GAGTC	4 cut(s)	234, 673, 1360, 2026
MmeI	TCCRAC	2 cut(s)	1218, 2101
Mox20I	TGGCCA	2 cut(s)	475, 782
MroI	TCCGGA	3 cut(s)	407, 2043, 2131
MroXI	GAANNNNTTC	1 cut(s)	272
MscI	TGGCCA	2 cut(s)	475, 782
MseI	TTAA	7 cut(s)	311, 357, 492, 603, 1608, 1878, 1971
MslI	CAYNNNNRTG	6 cut(s)	107, 523, 644, 909, 1086, 2153
Msp20I	TGGCCA	2 cut(s)	475, 782
MspA1I	CMGCKG	1 cut(s)	1043
MunI	CAATTG	3 cut(s)	66, 1050, 1945
Mva1269I	GAATGC	1 cut(s)	1991
MvaI	CCWGG	5 cut(s)	324, 744, 1220, 1501, 1530
MvnI	CGCG	1 cut(s)	1571
MwoI	GCNNNNNNNGC	8 cut(s)	324, 770, 779, 1274, 1448, 1655, 1805, 2295
NciI	CCSGG	5 cut(s)	718, 1122, 1810, 1842, 1843
NdeII	GATC	8 cut(s)	13, 142, 444, 916, 1054, 1371, 1706, 1966
NmuCI	GTSAC	2 cut(s)	1099, 2053
NsbI	TGCGCA	1 cut(s)	1077
NspI	RCATGY	3 cut(s)	779, 1817, 2261
OliI	CACNNNNGTG	1 cut(s)	2153
PagI	TCATGA	2 cut(s)	2035, 2292
PceI	AGGCCT	1 cut(s)	308
PciI	ACATGT	1 cut(s)	2257
PcsI	WCGNNNNNNNCGW	1 cut(s)	867
PctI	GAATGC	1 cut(s)	1991
PdmI	GAANNNNTTC	1 cut(s)	272
PfeI	GAWTC	3 cut(s)	113, 173, 625
PfoI	TCCNGGA	1 cut(s)	1120
PkrI	GCNGC	8 cut(s)	700, 838, 1042, 1549, 1573, 1660, 1665, 1699
PleI	GAGTC	4 cut(s)	234, 673, 1359, 2026
PpsI	GAGTC	4 cut(s)	234, 673, 1359, 2026
PpuMI	RGGWCCY	1 cut(s)	989
PscI	ACATGT	1 cut(s)	2257
PsiI	TTATAA	1 cut(s)	1254
Psp1406I	AACGTT	1 cut(s)	167
Psp5II	RGGWCCY	1 cut(s)	989
Psp6I	CCWGG	5 cut(s)	322, 742, 1218, 1499, 1528
PspEI	GGTNACC	1 cut(s)	2053
PspGI	CCWGG	5 cut(s)	322, 742, 1218, 1499, 1528
PspPI	GGNCC	8 cut(s)	989, 1107, 1440, 1481, 1503, 1831, 2072, 2192
PspPPI	RGGWCCY	1 cut(s)	989
PstNI	CAGNNNCTG	1 cut(s)	749
PsuI	RGATCY	3 cut(s)	916, 1706, 1966
PvuII	CAGCTG	1 cut(s)	1043
RsaI	GTAC	4 cut(s)	788, 1003, 1048, 2025
RsaNI	GTAC	4 cut(s)	787, 1002, 1047, 2024
RseI	CAYNNNNRTG	6 cut(s)	107, 523, 644, 909, 1086, 2153
SaqAI	TTAA	7 cut(s)	311, 357, 492, 603, 1608, 1878, 1971
SatI	GCNGC	8 cut(s)	699, 837, 1041, 1548, 1572, 1659, 1664, 1698
Sau3AI	GATC	8 cut(s)	13, 142, 444, 916, 1054, 1371, 1706, 1966
Sau96I	GGNCC	8 cut(s)	989, 1107, 1440, 1481, 1503, 1831, 2072, 2192
SchI	GAGTC	4 cut(s)	234, 673, 1360, 2026
SduI	GDGCHC	6 cut(s)	615, 1640, 1678, 1694, 1848, 1894
SfaNI	GCATC	7 cut(s)	114, 731, 1087, 1153, 1293, 1762, 1846
SgrAI	CRCCGGYG	1 cut(s)	464
SinI	GGWCC	4 cut(s)	989, 1107, 1503, 2192
SmaI	CCCGGG	1 cut(s)	1843
SmiMI	CAYNNNNRTG	6 cut(s)	107, 523, 644, 909, 1086, 2153
SmlI	CTYRAG	1 cut(s)	302
SmoI	CTYRAG	1 cut(s)	302
SpeI	ACTAGT	1 cut(s)	5
SseBI	AGGCCT	1 cut(s)	308
SsiI	CCGC	6 cut(s)	699, 809, 1338, 1571, 1658, 1710
SspMI	CTAG	8 cut(s)	6, 198, 768, 1476, 1788, 1796, 1835, 2063
StuI	AGGCCT	1 cut(s)	308
StyI	CCWWGG	3 cut(s)	945, 1022, 2062
TaaI	ACNGT	6 cut(s)	863, 1036, 1617, 2053, 2103, 2215
TaiI	ACGT	6 cut(s)	170, 873, 1238, 1878, 2025, 2088
TaqI	TCGA	2 cut(s)	1940, 2078
TatI	WGTACW	1 cut(s)	786
TauI	GCSGC	3 cut(s)	701, 1574, 1661
TfiI	GAWTC	3 cut(s)	113, 173, 625
Tru1I	TTAA	7 cut(s)	311, 357, 492, 603, 1608, 1878, 1971
Tru9I	TTAA	7 cut(s)	311, 357, 492, 603, 1608, 1878, 1971
TscAI	CASTG	8 cut(s)	126, 211, 283, 295, 345, 670, 760, 2220
TseFI	GTSAC	2 cut(s)	1099, 2053
TseI	GCWGC	5 cut(s)	836, 1040, 1547, 1663, 1697
Tsp45I	GTSAC	2 cut(s)	1099, 2053
TspGWI	ACGGA	3 cut(s)	447, 1087, 2165
TspMI	CCCGGG	1 cut(s)	1841
TspRI	CASTG	8 cut(s)	126, 211, 283, 295, 345, 670, 760, 2220
VneI	GTGCAC	2 cut(s)	1674, 1890
VpaK11BI	GGWCC	4 cut(s)	989, 1107, 1503, 2192
XagI	CCTNNNNNAGG	1 cut(s)	2066
XapI	RAATTY	4 cut(s)	649, 1061, 1126, 2039
XbaI	TCTAGA	1 cut(s)	1475
XceI	RCATGY	3 cut(s)	779, 1817, 2261
XmaI	CCCGGG	1 cut(s)	1841
XmaJI	CCTAGG	1 cut(s)	2062
XmiI	GTMKAC	1 cut(s)	692
XmnI	GAANNNNTTC	1 cut(s)	272
XspI	CTAG	8 cut(s)	6, 198, 768, 1476, 1788, 1796, 1835, 2063

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.