Rorug06G0364600

subtilisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001268
Physical Location & Seq
Forward (+)
51195275 .. 51196980
1706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0364600.1

Sequence Viewer

Length: 426 bp
ATGATGAAAGGTGTGGGAGGACCGCTTCTATGCATAGGCGATCTGCTGAGCGATGTGGGAGAATCAGATGCTTCACCTCCACCGCAACAACATGAGCCCTCGGTTTCTGCTCCTATTTCCACCTCTACTAGTCAAAACCCGGACCTTACCAAGTTGTTCCAGGAGAACTATGAGCAGCTGAATGAGGCGCTTGCCGGTACAGACCACTCATGGGCAGGTCTTACTCTGAAGCTATGCGGTGCTCTGGAAACTGCAAACAAGTTGGTTAAGACAACCAACTCAAATGTCACATTGTTGTCGGAGAAGGTTGGAGATCTTGGGGAAGTTATCAAGAGGGCAGATTCTGCCATAGCAGCAGCAAGGGTTGTCCATGCCTCACTTAATGAAAAAGAGGGTTCCCTCATTGGCAATCAAAATATCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

141

Amino Acids

14.76

Weight (kDa)

4.8

Isoelectric Point (pI)

24.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 206
AciI CCGC 3 cut(s) 23, 83, 237
AcuI CTGAAG 1 cut(s) 248
AfaI GTAC 1 cut(s) 199
AfiI CCNNNNNNNGG 1 cut(s) 211
AhlI ACTAGT 1 cut(s) 128
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 2 cut(s) 178, 232
AluI AGCT 2 cut(s) 178, 232
Alw21I GWGCWC 1 cut(s) 244
AlwNI CAGNNNCTG 1 cut(s) 344
ApeKI GCWGC 3 cut(s) 175, 353, 356
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 2 cut(s) 20, 142
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 66
AvaII GGWCC 2 cut(s) 20, 142
BanII GRGCYC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 244
BbvI GCAGC 3 cut(s) 187, 365, 368
BciT130I CCWGG 1 cut(s) 161
BcnI CCSGG 1 cut(s) 140
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 1 cut(s) 129
BfoI RGCGCY 1 cut(s) 191
BfuAI ACCTGC 1 cut(s) 206
BglII AGATCT 1 cut(s) 313
BisI GCNGC 3 cut(s) 176, 354, 357
BlpI GCTNAGC 1 cut(s) 47
BlsI GCNGC 3 cut(s) 177, 355, 358
Bme1390I CCNGG 2 cut(s) 140, 161
Bme18I GGWCC 2 cut(s) 20, 142
BmgT120I GGNCC 2 cut(s) 20, 142
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 2 cut(s) 140, 161
BmsI GCATC 1 cut(s) 58
Bpu1102I GCTNAGC 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 99
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse118I RCCGGY 1 cut(s) 194
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 1 cut(s) 38
BseXI GCAGC 3 cut(s) 187, 365, 368
BsiHKAI GWGCWC 1 cut(s) 244
BsiSI CCGG 2 cut(s) 140, 195
BslI CCNNNNNNNGG 1 cut(s) 211
Bsp1286I GDGCHC 2 cut(s) 99, 244
Bsp143I GATC 2 cut(s) 40, 313
Bsp1720I GCTNAGC 1 cut(s) 47
BspACI CCGC 3 cut(s) 23, 83, 237
BspCNI CTCAG 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 397
BspMI ACCTGC 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 194
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 2 cut(s) 40, 313
Bst2UI CCWGG 1 cut(s) 161
BstAPI GCANNNNNTGC 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 1 cut(s) 47
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 2 cut(s) 43, 316
BstMBI GATC 2 cut(s) 40, 313
BstMWI GCNNNNNNNGC 2 cut(s) 344, 353
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 2 cut(s) 138, 159
BstV1I GCAGC 3 cut(s) 187, 365, 368
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BtgZI GCGATG 1 cut(s) 66
BveI ACCTGC 1 cut(s) 206
Cac8I GCNNGC 1 cut(s) 192
CaiI CAGNNNCTG 1 cut(s) 344
CfoI GCGC 1 cut(s) 190
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 2 cut(s) 20, 142
Csp6I GTAC 1 cut(s) 198
CviAII CATG 3 cut(s) 92, 210, 371
CviJI RGCY 3 cut(s) 97, 178, 232
CviKI_1 RGCY 3 cut(s) 97, 178, 232
CviQI GTAC 1 cut(s) 198
DdeI CTNAG 1 cut(s) 47
DpnI GATC 2 cut(s) 42, 315
DpnII GATC 2 cut(s) 40, 313
Eco24I GRGCYC 1 cut(s) 99
Eco47I GGWCC 2 cut(s) 20, 142
Eco57I CTGAAG 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 159
EcoT22I ATGCAT 1 cut(s) 35
EcoT38I GRGCYC 1 cut(s) 99
FaeI CATG 3 cut(s) 95, 213, 374
FaiI YATR 8 cut(s) 31, 35, 93, 171, 211, 235, 350, 372
FatI CATG 3 cut(s) 91, 209, 370
Fnu4HI GCNGC 3 cut(s) 176, 354, 357
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 3 cut(s) 176, 354, 357
FspBI CTAG 1 cut(s) 129
GlaI GCGC 1 cut(s) 189
GluI GCNGC 3 cut(s) 176, 354, 357
HaeII RGCGCY 1 cut(s) 191
HapII CCGG 2 cut(s) 140, 195
HhaI GCGC 1 cut(s) 190
Hin1II CATG 3 cut(s) 95, 213, 374
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HinfI GANTC 2 cut(s) 62, 341
HpaII CCGG 2 cut(s) 140, 195
HphI GGTGA 1 cut(s) 66
Hpy188I TCNGA 3 cut(s) 67, 228, 301
Hpy188III TCNNGA 2 cut(s) 245, 331
HpyAV CCTTC 1 cut(s) 298
HpyCH4V TGCA 2 cut(s) 33, 254
HpyF10VI GCNNNNNNNGC 2 cut(s) 344, 353
HpyF3I CTNAG 1 cut(s) 47
Hsp92II CATG 3 cut(s) 95, 213, 374
HspAI GCGC 1 cut(s) 188
Kzo9I GATC 2 cut(s) 40, 313
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 6 cut(s) 146, 153, 173, 201, 208, 230
Lsp1109I GCAGC 3 cut(s) 187, 365, 368
LweI GCATC 1 cut(s) 58
MaeI CTAG 1 cut(s) 129
MaeIII GTNAC 1 cut(s) 286
MalI GATC 2 cut(s) 42, 315
MboI GATC 2 cut(s) 40, 313
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 2 cut(s) 99, 244
MmeI TCCRAC 2 cut(s) 279, 289
MnlI CCTC 9 cut(s) 11, 87, 109, 133, 178, 327, 385, 385, 410
Mph1103I ATGCAT 1 cut(s) 35
MseI TTAA 2 cut(s) 267, 381
MspA1I CMGCKG 1 cut(s) 178
MspI CCGG 2 cut(s) 140, 195
MspR9I CCNGG 2 cut(s) 140, 161
MvaI CCWGG 1 cut(s) 161
MwoI GCNNNNNNNGC 2 cut(s) 344, 353
NciI CCSGG 1 cut(s) 140
NdeII GATC 2 cut(s) 40, 313
NlaIII CATG 3 cut(s) 95, 213, 374
NlaIV GGNNCC 1 cut(s) 397
NmuCI GTSAC 1 cut(s) 286
NsiI ATGCAT 1 cut(s) 35
PfeI GAWTC 2 cut(s) 62, 341
PfoI TCCNGGA 1 cut(s) 159
PkrI GCNGC 3 cut(s) 177, 355, 358
Psp6I CCWGG 1 cut(s) 159
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 1 cut(s) 397
PspPI GGNCC 2 cut(s) 20, 142
PstNI CAGNNNCTG 1 cut(s) 344
PsuI RGATCY 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 1 cut(s) 199
RsaNI GTAC 1 cut(s) 198
SaqAI TTAA 2 cut(s) 267, 381
SatI GCNGC 3 cut(s) 176, 354, 357
Sau3AI GATC 2 cut(s) 40, 313
Sau96I GGNCC 2 cut(s) 20, 142
ScrFI CCNGG 2 cut(s) 140, 161
SduI GDGCHC 2 cut(s) 99, 244
SetI ASST 8 cut(s) 13, 79, 125, 147, 180, 220, 234, 309
SfaNI GCATC 1 cut(s) 58
SinI GGWCC 2 cut(s) 20, 142
SpeI ACTAGT 1 cut(s) 128
SsiI CCGC 3 cut(s) 23, 83, 237
SspMI CTAG 1 cut(s) 129
StyD4I CCNGG 2 cut(s) 138, 159
TfiI GAWTC 2 cut(s) 62, 341
Tru1I TTAA 2 cut(s) 267, 381
Tru9I TTAA 2 cut(s) 267, 381
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 3 cut(s) 175, 353, 356
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 2 cut(s) 20, 399
VpaK11BI GGWCC 2 cut(s) 20, 142
XspI CTAG 1 cut(s) 129
Zsp2I ATGCAT 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.