Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0001268

Physical Location & Seq

Genomic Coordinates Forward (+)

25445260 .. 25448355

Gene Structure 3096 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g34170.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2304 bp

ATGGATACTAATAACCACCATATTCTCTTCTATTTTTGGCTTTCCATTGTGACAATCTCATACCTTGCCACAGTCTCCACCTTCGCTCATGAATCCAGCAATTATATCATCTACATGGATGCATCATTTATGCCCAAAGCCTTTGCTGATCACCATTCTTGGTACTTGTCCACAGTGGACTCTGTACTATCCACTTCTTCCATCTCTTCTAAGCTCATATATAGCTACACTCATGTCTTAAACGGGTTCAGTGCAAGTCTCTCTGTTTCTGAGCTGGAGGCCTTGAAAACCTCTCCGGGTTACATATCTTCAGTTAGAGACTTGCCGGCAAAACCAGACACGACGCACTCGTCCCAGTTTCTTGGCCTTAACTCCAAGACAGGAGCATGGCCGGTGTCCAATTACGGCAAAGGCACCATTATTGGTTTGGTGGACTCCGGTGTTTGGCCAGAAAACGAAAGCTTTAATGACGGTGGAATGTCAGAAATCCCATCAAGATGGAAAGGAGAATGTGAAAGTGGAATGCAATTCAGCTCTTCACTATGCAACAAAAAGCTCATAGGGGCAAGGTTCTTTAACAAAGGCTTAGCAGCAGCAGACCCCAATGTGACATTTACGATGAACTCCACAAGGGACACCGACGGCCACGGGACCCACACCTCTTCTACGGCCGCTGGAAATTATGTAGCCGGAGCATCGTACTTTGGTTATGCCCCGGGTACTGCCAGCGGCATGGCGCCTCAGGCTCACGTGGCCATGTACAAGGCTTTGTGGGACGAGGGCGTCTTGTCGTCTGATATAATTGCGGCCATCGAGCAAGCAGTTGTGGACGGTGTGGATGTCTTATCCCTATCGTTCGGATTCGATGGCATTCCGCTGTACGAGGACCCTGTTGCCATAGCCACATTCACAGCCATGGAGAAGGGTGTCTTTGTTTCCACATCGGCAGGAAATGATGGACCTTACTATGGTAGTTTGCATAATGGGATCCCTTGGGTTCTAACAGTTGCAGCTAGTACCGTTGATCGTGACTTTCAAGGAACTGCACACCTTGGAAATGGAGAATCTGTCACAGGATTGACTCTTTTTCCGGGGGTTAATTCGTCTTCAACTCCAATCCCAATGGTTTTCATGAATGCATGTGACAGTTTAAAGAAGTTAAACCAGGTTGGGAAGAAGATCGTTGTGTGCCAGGACAACAGTTCAATTGTGGACCAATATCTTAATGTTCGAGATGCAAATGTTGCCGGAGGTATCTTCATCACCAACAACACCGACTTGGAATTGTTCCTCCAGAGCCAATTTCCCACACTTTTTCTGAGTCCCAAGGATGGTGAAACTATCAAAGACTACATCAAGAGCAACTCTAACCCGAAAGTAAGCTTTGAGTTTCAAAAGACAAGACTTGGTGTTAGACCAGCACCCACTGTGACTAGTTACACATCTAGAGGTCCATCTTACAGCTTCCCCTTTACTCTCAAGCCAGACATTACAGCACCCGGGTCACTAATCCTCGCTGCATGGCCTCGAAACATTTCGGTAGCGAGGATTAACAAGACTCAGGACCTGTTTAGCGAATTCAACTTGTTGTCGGGGACATCCATGGCGTGCCCGCATGCAGCTGGGCTAGCCGCGCTTGTGAAAGTTGCACATCCTGAATGGAGTCCTGCAGCTATCAGATCAGCTATGATGACTACATCGGATATCTTGGATAACACACTTAACCCCATTAAAGACATTGGGGATGATTTGCAACCGGCTAGTCCTCTTGCCATGGGAGCAGGTCATGTCAATCCCAACAAGGCCCTTAACCCGGGCCTCATATACGACGCGACCATAGATGATTATGTGAACCTTCTTTGTGCACTAAACTACACCCAGAAACACATCCAAATCATCACCGGATCGGCCTCGAACAATTGTTCAACTCCGTCATTAGATATAAACTACCCTTCGTTCATTGCATTTTTCAATGCTAATGATTCAATGTCGGGCGTACAAGCAACTCAAGAGTTCAAGAGGACGGTGACCAATGTAGGAAAGGGGCAGTCGACATACGTTGCAAGCGTGACAGCAATGAAAGGATTTGAAGTTGGAGTGGTTCCAAACAAATTGGTTTTCGGGAGGGAAGGTGAGAAGCTGAGCTTTAAAATGAGTATAAGAGGTCGAAGACTGATGGAAGAGACGGAGGCTTTTGGTTACCTGACTTGGGTGGACAGTGAAGGCCAACATGTGGTGAGAAGCCCCATTGTAGCCACAAGACTCAGCTCGGATGTAGTTATGTCATCGTCACGCAACCACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

768

Amino Acids

82.6

Weight (kDa)

5.7

Isoelectric Point (pI)

34.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	34 - 108	1.4e-14	Peptidase inhibitor I9
Peptidase_S8	PF00082	136 - 572	4.9e-44	Subtilase family
fn3_6	PF17766	647 - 751	7.1e-27	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001268)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67090
fragaria_vesca	FvH4_1g16180 FvH4_2g34170 FvH4_2g34170 FvH4_2g34230 FvH4_6g01060 FvH4_6g01061
malus_domestica	MD04G1236800.v1.1 MD04G1236900.v1.1 MD08G1098100.v1.1 MD15G1080600.v1.1 MD15G1285300.v1.1
prunus_persica	Prupe.1G433300_v2.0.a1 Prupe.6G168400_v2.0.a1 Prupe.6G356800_v2.0.a1 Prupe.7G131900_v2.0.a1
pyrus_communis	pycom04g21060 pycom04g21070 pycom08g07990 pycom12g23460 pycom15g07490 pycom15g24800
rosa_chinensis	RchiOBHm_Chr2g0105861 RchiOBHm_Chr2g0105881 RchiOBHm_Chr3g0448571 RchiOBHm_Chr6g0308191
rosa_laevigata	RLG00000010660 RLG00000017466 RLG00000017467 RLG00000025886 RLG00000025888 RLG00000025889 RLG00000025890
rosa_multiflora	Rmu_sc0003116.1_g000043 Rmu_sc0004367.1_g000007 Rmu_sc0004367.1_g000008 Rmu_sc0005706.1_g000016 Rmu_sc0010389.1_g000023
rosa_roxburghii	Rroxscaffold_2G00137780 Rroxscaffold_6G00426820 Rroxscaffold_6G00426860 Rroxscaffold_7G00160370 Rroxscaffold_7G00160460
rosa_rugosa	Rorug02G0131300 Rorug02G0131500 Rorug02G0613500 Rorug02G0613600 Rorug06G0364600
rosa_samantha	Rh2CG186700 Rh3DG013500 Rh6DG475800
rosa_wichuraiana	Rw2G014280 Rw2G014290 Rw3G000970 Rw6G041320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	349, 782
Acc36I	ACCTGC	1 cut(s)	1772
AccB1I	GGYRCC	2 cut(s)	413, 736
AccB7I	CCANNNNNTGG	1 cut(s)	2233
AccI	GTMKAC	1 cut(s)	2051
AccII	CGCG	2 cut(s)	1634, 1832
AciI	CCGC	6 cut(s)	672, 729, 806, 875, 1613, 1632
AclWI	GGATC	3 cut(s)	982, 995, 1912
AcoI	YGGCCR	6 cut(s)	389, 446, 643, 669, 753, 807
AcsI	RAATTY	1 cut(s)	1577
AcuI	CTGAAG	1 cut(s)	294
AcvI	CACGTG	1 cut(s)	751
AcyI	GRCGYC	2 cut(s)	737, 783
AfaI	GTAC	8 cut(s)	164, 186, 701, 721, 761, 881, 1018, 1998
AfiI	CCNNNNNNNGG	6 cut(s)	444, 968, 1331, 1813, 2209, 2233
AflIII	ACRYGT	1 cut(s)	2230
AhlI	ACTAGT	1 cut(s)	1433
AjnI	CCWGG	2 cut(s)	1164, 1191
Alw21I	GWGCWC	1 cut(s)	1867
Alw26I	GTCTC	4 cut(s)	79, 263, 312, 2177
Alw44I	GTGCAC	1 cut(s)	1863
AlwI	GGATC	3 cut(s)	982, 995, 1912
AlwNI	CAGNNNCTG	1 cut(s)	1567
Ama87I	CYCGRG	3 cut(s)	715, 1499, 1813
ApaLI	GTGCAC	1 cut(s)	1863
ApeKI	GCWGC	6 cut(s)	590, 593, 1010, 1517, 1619, 1670
ApoI	RAATTY	1 cut(s)	1577
Asp700I	GAANNNNTTC	1 cut(s)	1534
AspLEI	GCGC	2 cut(s)	739, 1636
AspS9I	GGNCC	8 cut(s)	651, 886, 959, 1213, 1451, 1564, 1804, 1816
AsuC2I	CCSGG	8 cut(s)	297, 716, 717, 1092, 1500, 1501, 1814, 1815
AsuHPI	GGTGA	7 cut(s)	143, 1255, 1346, 1891, 2038, 2144, 2248
AsuNHI	GCTAGC	1 cut(s)	1627
AvaI	CYCGRG	3 cut(s)	715, 1499, 1813
AvaII	GGWCC	6 cut(s)	651, 886, 959, 1213, 1451, 1564
AxyI	CCTNAGG	1 cut(s)	741
BaeGI	GKGCMC	2 cut(s)	1613, 1867
BalI	TGGCCA	2 cut(s)	448, 755
BamHI	GGATCC	1 cut(s)	987
BanI	GGYRCC	2 cut(s)	413, 736
BbrPI	CACGTG	1 cut(s)	751
BbsI	GAAGAC	2 cut(s)	1098, 2176
Bbv12I	GWGCWC	1 cut(s)	1867
BbvI	GCAGC	6 cut(s)	602, 605, 1022, 1504, 1631, 1682
BccI	CCATC	9 cut(s)	209, 492, 499, 818, 860, 950, 1325, 1462, 2170
BceAI	ACGGC	3 cut(s)	421, 658, 684
BciT130I	CCWGG	2 cut(s)	1166, 1193
BclI	TGATCA	1 cut(s)	148
BcnI	CCSGG	8 cut(s)	297, 716, 717, 1092, 1500, 1501, 1814, 1815
BcoDI	GTCTC	4 cut(s)	79, 263, 312, 2177
BcuI	ACTAGT	1 cut(s)	1433
BfaI	CTAG	6 cut(s)	1014, 1434, 1446, 1628, 1761, 2302
BfmI	CTRYAG	1 cut(s)	1668
BfoI	RGCGCY	1 cut(s)	740
BfuAI	ACCTGC	1 cut(s)	1772
BlpI	GCTNAGC	2 cut(s)	586, 2141
Bme18I	GGWCC	6 cut(s)	651, 886, 959, 1213, 1451, 1564
BmeT110I	CYCGRG	3 cut(s)	715, 1499, 1813
BmgT120I	GGNCC	8 cut(s)	651, 886, 959, 1213, 1451, 1564, 1804, 1816
BmiI	GGNNCC	7 cut(s)	415, 652, 653, 738, 888, 989, 2103
BmrI	ACTGGG	1 cut(s)	349
BmsI	GCATC	4 cut(s)	109, 131, 704, 1225
BmtI	GCTAGC	1 cut(s)	1631
BmuI	ACTGGG	1 cut(s)	349
BpiI	GAAGAC	2 cut(s)	1098, 2176
BpmI	CTGGAG	2 cut(s)	296, 1277
Bpu1102I	GCTNAGC	2 cut(s)	586, 2141
BpuEI	CTTGAG	2 cut(s)	1463, 1992
BpuMI	CCSGG	8 cut(s)	297, 716, 717, 1092, 1500, 1501, 1814, 1815
BsaAI	YACGTR	1 cut(s)	751
BsaHI	GRCGYC	2 cut(s)	737, 783
BsaWI	WCCGGW	2 cut(s)	437, 1901
BsaXI	ACNNNNNCTCC	4 cut(s)	1053, 1083, 2116, 2146
Bsc4I	CCNNNNNNNGG	6 cut(s)	444, 968, 1331, 1813, 2209, 2233
Bse118I	RCCGGY	3 cut(s)	325, 391, 1756
Bse1I	ACTGG	1 cut(s)	355
Bse21I	CCTNAGG	1 cut(s)	741
Bse3DI	GCAATG	2 cut(s)	1959, 2082
BseBI	CCWGG	2 cut(s)	1166, 1193
BseGI	GGATG	8 cut(s)	124, 844, 1336, 1598, 1651, 1750, 1887, 2278
BseLI	CCNNNNNNNGG	6 cut(s)	444, 968, 1331, 1813, 2209, 2233
BseMI	GCAATG	2 cut(s)	1959, 2082
BseMII	CTCAG	6 cut(s)	261, 755, 1310, 1574, 2132, 2278
BseNI	ACTGG	1 cut(s)	355
BseSI	GKGCMC	2 cut(s)	1613, 1867
BseX3I	CGGCCG	1 cut(s)	669
BseXI	GCAGC	6 cut(s)	602, 605, 1022, 1504, 1631, 1682
BseYI	CCCAGC	1 cut(s)	1622
BsgI	GTGCAG	1 cut(s)	1029
Bsh1236I	CGCG	2 cut(s)	1634, 1832
Bsh1285I	CGRYCG	1 cut(s)	672
BshNI	GGYRCC	2 cut(s)	413, 736
BsiEI	CGRYCG	1 cut(s)	672
BsiHKAI	GWGCWC	1 cut(s)	1867
BsiHKCI	CYCGRG	3 cut(s)	715, 1499, 1813
BslFI	GGGAC	6 cut(s)	337, 647, 664, 788, 1308, 1609
BslI	CCNNNNNNNGG	6 cut(s)	444, 968, 1331, 1813, 2209, 2233
BsmAI	GTCTC	4 cut(s)	79, 263, 312, 2177
BsmBI	CGTCTC	1 cut(s)	2177
BsmFI	GGGAC	6 cut(s)	337, 647, 664, 788, 1308, 1609
BsmI	GAATGC	3 cut(s)	528, 870, 1141
BsoBI	CYCGRG	3 cut(s)	715, 1499, 1813
Bsp1286I	GDGCHC	2 cut(s)	1613, 1867
Bsp1407I	TGTACA	1 cut(s)	759
Bsp143I	GATC	6 cut(s)	148, 987, 1024, 1179, 1679, 1904
Bsp1720I	GCTNAGC	2 cut(s)	586, 2141
Bsp19I	CCATGG	3 cut(s)	915, 1602, 1773
BspACI	CCGC	6 cut(s)	672, 729, 806, 875, 1613, 1632
BspCNI	CTCAG	6 cut(s)	262, 754, 1311, 1573, 2133, 2277
BspFNI	CGCG	2 cut(s)	1634, 1832
BspHI	TCATGA	2 cut(s)	88, 1131
BspLI	GGNNCC	7 cut(s)	415, 652, 653, 738, 888, 989, 2103
BspMAI	CTGCAG	1 cut(s)	1672
BspMI	ACCTGC	1 cut(s)	1772
BspOI	GCTAGC	1 cut(s)	1631
BspPI	GGATC	3 cut(s)	982, 995, 1912
BspQI	GCTCTTC	1 cut(s)	541
BspT107I	GGYRCC	2 cut(s)	413, 736
BsrDI	GCAATG	2 cut(s)	1959, 2082
BsrFI	RCCGGY	3 cut(s)	325, 391, 1756
BsrGI	TGTACA	1 cut(s)	759
BsrI	ACTGG	1 cut(s)	355
BssAI	RCCGGY	3 cut(s)	325, 391, 1756
BssMI	GATC	6 cut(s)	148, 987, 1024, 1179, 1679, 1904
BssNI	GRCGYC	2 cut(s)	737, 783
BssT1I	CCWWGG	6 cut(s)	915, 992, 1051, 1326, 1602, 1773
Bst2UI	CCWGG	2 cut(s)	1166, 1193
Bst6I	CTCTTC	5 cut(s)	32, 211, 541, 667, 2175
BstACI	GRCGYC	2 cut(s)	737, 783
BstAPI	GCANNNNNTGC	1 cut(s)	1244
BstAUI	TGTACA	1 cut(s)	759
BstBAI	YACGTR	1 cut(s)	751
BstC8I	GCNNGC	8 cut(s)	327, 727, 819, 1609, 1613, 1617, 1629, 2065
BstDEI	CTNAG	8 cut(s)	210, 270, 586, 741, 1319, 1560, 2141, 2264
BstDSI	CCRYGG	4 cut(s)	646, 915, 1602, 1773
BstEII	GGTNACC	2 cut(s)	2026, 2198
BstF5I	GGATG	8 cut(s)	124, 844, 1336, 1598, 1651, 1750, 1887, 2278
BstFNI	CGCG	2 cut(s)	1634, 1832
BstH2I	RGCGCY	1 cut(s)	740
BstHHI	GCGC	2 cut(s)	739, 1636
BstKTI	GATC	6 cut(s)	151, 990, 1027, 1182, 1682, 1907
BstMAI	GTCTC	4 cut(s)	79, 263, 312, 2177
BstMBI	GATC	6 cut(s)	148, 987, 1024, 1179, 1679, 1904
BstMCI	CGRYCG	1 cut(s)	672
BstMWI	GCNNNNNNNGC	6 cut(s)	743, 752, 1244, 1628, 1633, 1778
BstNI	CCWGG	2 cut(s)	1166, 1193
BstNSI	RCATGY	3 cut(s)	1143, 1619, 2234
BstPI	GGTNACC	2 cut(s)	2026, 2198
BstSFI	CTRYAG	1 cut(s)	1668
BstSLI	GKGCMC	2 cut(s)	1613, 1867
BstUI	CGCG	2 cut(s)	1634, 1832
BstV1I	GCAGC	6 cut(s)	602, 605, 1022, 1504, 1631, 1682
BstV2I	GAAGAC	2 cut(s)	1098, 2176
BstX2I	RGATCY	1 cut(s)	987
BstXI	CCANNNNNNTGG	3 cut(s)	362, 498, 733
BstYI	RGATCY	1 cut(s)	987
BstZI	CGGCCG	1 cut(s)	669
Bsu36I	CCTNAGG	1 cut(s)	741
BtgI	CCRYGG	4 cut(s)	646, 915, 1602, 1773
BtsCI	GGATG	8 cut(s)	124, 844, 1336, 1598, 1651, 1750, 1887, 2278
BtsIMutI	CAGTG	4 cut(s)	180, 256, 1425, 2224
BveI	ACCTGC	1 cut(s)	1772
Cac8I	GCNNGC	8 cut(s)	327, 727, 819, 1609, 1613, 1617, 1629, 2065
CaiI	CAGNNNCTG	1 cut(s)	1567
CciI	TCATGA	2 cut(s)	88, 1131
CfoI	GCGC	2 cut(s)	739, 1636
Cfr10I	RCCGGY	3 cut(s)	325, 391, 1756
Cfr13I	GGNCC	8 cut(s)	651, 886, 959, 1213, 1451, 1564, 1804, 1816
Cfr9I	CCCGGG	3 cut(s)	715, 1499, 1813
CseI	GACGC	3 cut(s)	352, 772, 1838
CsiI	ACCWGGT	1 cut(s)	1164
Csp6I	GTAC	8 cut(s)	163, 185, 700, 720, 760, 880, 1017, 1997
CviQI	GTAC	8 cut(s)	163, 185, 700, 720, 760, 880, 1017, 1997
DdeI	CTNAG	8 cut(s)	210, 270, 586, 741, 1319, 1560, 2141, 2264
DinI	GGCGCC	1 cut(s)	738
DpnI	GATC	6 cut(s)	150, 989, 1026, 1181, 1681, 1906
DpnII	GATC	6 cut(s)	148, 987, 1024, 1179, 1679, 1904
DraI	TTTAAA	2 cut(s)	1152, 2149
DrdI	GACNNNNNNGTC	2 cut(s)	349, 782
DseDI	GACNNNNNNGTC	2 cut(s)	349, 782
EaeI	YGGCCR	6 cut(s)	389, 446, 643, 669, 753, 807
EagI	CGGCCG	1 cut(s)	669
Eam1104I	CTCTTC	5 cut(s)	32, 211, 541, 667, 2175
EarI	CTCTTC	5 cut(s)	32, 211, 541, 667, 2175
EclXI	CGGCCG	1 cut(s)	669
Eco130I	CCWWGG	6 cut(s)	915, 992, 1051, 1326, 1602, 1773
Eco147I	AGGCCT	1 cut(s)	281
Eco32I	GATATC	1 cut(s)	1705
Eco47I	GGWCC	6 cut(s)	651, 886, 959, 1213, 1451, 1564
Eco52I	CGGCCG	1 cut(s)	669
Eco57I	CTGAAG	1 cut(s)	294
Eco72I	CACGTG	1 cut(s)	751
Eco81I	CCTNAGG	1 cut(s)	741
Eco88I	CYCGRG	3 cut(s)	715, 1499, 1813
Eco91I	GGTNACC	2 cut(s)	2026, 2198
EcoO109I	RGGNCCY	4 cut(s)	651, 886, 1564, 1804
EcoO65I	GGTNACC	2 cut(s)	2026, 2198
EcoRI	GAATTC	1 cut(s)	1577
EcoRII	CCWGG	2 cut(s)	1164, 1191
EcoRV	GATATC	1 cut(s)	1705
EcoT14I	CCWWGG	6 cut(s)	915, 992, 1051, 1326, 1602, 1773
EcoT22I	ATGCAT	2 cut(s)	124, 1141
EgeI	GGCGCC	1 cut(s)	738
EheI	GGCGCC	1 cut(s)	738
ErhI	CCWWGG	6 cut(s)	915, 992, 1051, 1326, 1602, 1773
Esp3I	CGTCTC	1 cut(s)	2177
FalI	AAGNNNNNCTT	4 cut(s)	914, 946, 2129, 2161
FaqI	GGGAC	6 cut(s)	337, 647, 664, 788, 1308, 1609
FauI	CCCGC	1 cut(s)	1620
FbaI	TGATCA	1 cut(s)	148
FblI	GTMKAC	1 cut(s)	2051
FokI	GGATG	8 cut(s)	131, 851, 1343, 1585, 1638, 1757, 1874, 2285
FspBI	CTAG	6 cut(s)	1014, 1434, 1446, 1628, 1761, 2302
GlaI	GCGC	2 cut(s)	738, 1635
GsaI	CCCAGC	1 cut(s)	1626
GsuI	CTGGAG	2 cut(s)	296, 1277
HaeII	RGCGCY	1 cut(s)	740
HgaI	GACGC	3 cut(s)	352, 772, 1838
HhaI	GCGC	2 cut(s)	739, 1636
Hin1I	GRCGYC	2 cut(s)	737, 783
Hin6I	GCGC	2 cut(s)	737, 1634
HinP1I	GCGC	2 cut(s)	737, 1634
HincII	GTYRAC	1 cut(s)	2052
HindII	GTYRAC	1 cut(s)	2052
HindIII	AAGCTT	2 cut(s)	460, 1381
HphI	GGTGA	7 cut(s)	143, 1255, 1346, 1891, 2038, 2144, 2248
Hpy166II	GTNNAC	9 cut(s)	171, 178, 433, 829, 1213, 1852, 1865, 2052, 2215
Hpy188I	TCNGA	8 cut(s)	271, 484, 796, 860, 1320, 1679, 1702, 2272
Hpy8I	GTNNAC	9 cut(s)	171, 178, 433, 829, 1213, 1852, 1865, 2052, 2215
Hpy99I	CGWCG	3 cut(s)	346, 644, 1832
HpyAV	CCTTC	6 cut(s)	91, 916, 1865, 1962, 2123, 2216
HpyCH4IV	ACGT	2 cut(s)	750, 2058
HpyF10VI	GCNNNNNNNGC	6 cut(s)	743, 752, 1244, 1628, 1633, 1778
HpyF3I	CTNAG	8 cut(s)	210, 270, 586, 741, 1319, 1560, 2141, 2264
HpySE526I	ACGT	2 cut(s)	750, 2058
Hsp92I	GRCGYC	2 cut(s)	737, 783
HspAI	GCGC	2 cut(s)	737, 1634
KasI	GGCGCC	1 cut(s)	736
KflI	GGGWCCC	1 cut(s)	651
KroI	GCCGGC	1 cut(s)	325
KroNI	GCCGGC	1 cut(s)	327
Ksp22I	TGATCA	1 cut(s)	148
Kzo9I	GATC	6 cut(s)	148, 987, 1024, 1179, 1679, 1904
LguI	GCTCTTC	1 cut(s)	541
LmnI	GCTCC	3 cut(s)	383, 692, 1778
Lsp1109I	GCAGC	6 cut(s)	602, 605, 1022, 1504, 1631, 1682
LweI	GCATC	4 cut(s)	109, 131, 704, 1225
MabI	ACCWGGT	1 cut(s)	1164
MaeI	CTAG	6 cut(s)	1014, 1434, 1446, 1628, 1761, 2302
MaeII	ACGT	2 cut(s)	750, 2058
MalI	GATC	6 cut(s)	150, 989, 1026, 1181, 1681, 1906
MboI	GATC	6 cut(s)	148, 987, 1024, 1179, 1679, 1904
MfeI	CAATTG	2 cut(s)	1206, 1918
MflI	RGATCY	1 cut(s)	987
MhlI	GDGCHC	2 cut(s)	1613, 1867
MlsI	TGGCCA	2 cut(s)	448, 755
MluNI	TGGCCA	2 cut(s)	448, 755
Mly113I	GGCGCC	1 cut(s)	737
MlyI	GAGTC	7 cut(s)	173, 428, 1075, 1330, 1552, 1672, 2256
MmeI	TCCRAC	1 cut(s)	2074
Mox20I	TGGCCA	2 cut(s)	448, 755
Mph1103I	ATGCAT	2 cut(s)	124, 1141
MroNI	GCCGGC	1 cut(s)	325
MroXI	GAANNNNTTC	1 cut(s)	1534
MscI	TGGCCA	2 cut(s)	448, 755
MslI	CAYNNNNRTG	3 cut(s)	113, 496, 914
Msp20I	TGGCCA	2 cut(s)	448, 755
MspA1I	CMGCKG	4 cut(s)	674, 729, 877, 1622
MunI	CAATTG	2 cut(s)	1206, 1918
Mva1269I	GAATGC	3 cut(s)	528, 870, 1141
MvaI	CCWGG	2 cut(s)	1166, 1193
MvnI	CGCG	2 cut(s)	1634, 1832
MwoI	GCNNNNNNNGC	6 cut(s)	743, 752, 1244, 1628, 1633, 1778
NaeI	GCCGGC	1 cut(s)	327
NarI	GGCGCC	1 cut(s)	737
NciI	CCSGG	8 cut(s)	297, 716, 717, 1092, 1500, 1501, 1814, 1815
NcoI	CCATGG	3 cut(s)	915, 1602, 1773
NdeII	GATC	6 cut(s)	148, 987, 1024, 1179, 1679, 1904
NgoMIV	GCCGGC	1 cut(s)	325
NheI	GCTAGC	1 cut(s)	1627
NlaIV	GGNNCC	7 cut(s)	415, 652, 653, 738, 888, 989, 2103
NsiI	ATGCAT	2 cut(s)	124, 1141
NspI	RCATGY	3 cut(s)	1143, 1619, 2234
PaeI	GCATGC	1 cut(s)	1619
PagI	TCATGA	2 cut(s)	88, 1131
PceI	AGGCCT	1 cut(s)	281
PciI	ACATGT	1 cut(s)	2230
PciSI	GCTCTTC	1 cut(s)	541
PcsI	WCGNNNNNNNCGW	2 cut(s)	347, 2064
PctI	GAATGC	3 cut(s)	528, 870, 1141
PdiI	GCCGGC	1 cut(s)	327
PdmI	GAANNNNTTC	1 cut(s)	1534
PfeI	GAWTC	4 cut(s)	92, 861, 1064, 1982
PflMI	CCANNNNNTGG	1 cut(s)	2233
PleI	GAGTC	7 cut(s)	173, 428, 1075, 1329, 1552, 1671, 2256
PluTI	GGCGCC	1 cut(s)	740
PmaCI	CACGTG	1 cut(s)	751
PmlI	CACGTG	1 cut(s)	751
PpsI	GAGTC	7 cut(s)	173, 428, 1075, 1329, 1552, 1671, 2256
Ppu21I	YACGTR	1 cut(s)	751
PpuMI	RGGWCCY	3 cut(s)	651, 886, 1564
PscI	ACATGT	1 cut(s)	2230
Psp5II	RGGWCCY	3 cut(s)	651, 886, 1564
Psp6I	CCWGG	2 cut(s)	1164, 1191
PspCI	CACGTG	1 cut(s)	751
PspEI	GGTNACC	2 cut(s)	2026, 2198
PspFI	CCCAGC	1 cut(s)	1622
PspGI	CCWGG	2 cut(s)	1164, 1191
PspN4I	GGNNCC	7 cut(s)	415, 652, 653, 738, 888, 989, 2103
PspPI	GGNCC	8 cut(s)	651, 886, 959, 1213, 1451, 1564, 1804, 1816
PspPPI	RGGWCCY	3 cut(s)	651, 886, 1564
PstI	CTGCAG	1 cut(s)	1672
PstNI	CAGNNNCTG	1 cut(s)	1567
PsuI	RGATCY	1 cut(s)	987
PvuII	CAGCTG	1 cut(s)	1622
RsaI	GTAC	8 cut(s)	164, 186, 701, 721, 761, 881, 1018, 1998
RsaNI	GTAC	8 cut(s)	163, 185, 700, 720, 760, 880, 1017, 1997
RseI	CAYNNNNRTG	3 cut(s)	113, 496, 914
SalI	GTCGAC	1 cut(s)	2050
SapI	GCTCTTC	1 cut(s)	541
Sau3AI	GATC	6 cut(s)	148, 987, 1024, 1179, 1679, 1904
Sau96I	GGNCC	8 cut(s)	651, 886, 959, 1213, 1451, 1564, 1804, 1816
SchI	GAGTC	7 cut(s)	173, 428, 1075, 1330, 1552, 1672, 2256
SduI	GDGCHC	2 cut(s)	1613, 1867
SexAI	ACCWGGT	1 cut(s)	1164
SfaNI	GCATC	4 cut(s)	109, 131, 704, 1225
SfcI	CTRYAG	1 cut(s)	1668
SfoI	GGCGCC	1 cut(s)	738
SinI	GGWCC	6 cut(s)	651, 886, 959, 1213, 1451, 1564
SmaI	CCCGGG	3 cut(s)	717, 1501, 1815
SmiMI	CAYNNNNRTG	3 cut(s)	113, 496, 914
SmlI	CTYRAG	2 cut(s)	1478, 2007
SmoI	CTYRAG	2 cut(s)	1478, 2007
SpeI	ACTAGT	1 cut(s)	1433
SphI	GCATGC	1 cut(s)	1619
SseBI	AGGCCT	1 cut(s)	281
SsiI	CCGC	6 cut(s)	672, 729, 806, 875, 1613, 1632
SspDI	GGCGCC	1 cut(s)	736
SspMI	CTAG	6 cut(s)	1014, 1434, 1446, 1628, 1761, 2302
StuI	AGGCCT	1 cut(s)	281
StyI	CCWWGG	6 cut(s)	915, 992, 1051, 1326, 1602, 1773
TaiI	ACGT	2 cut(s)	753, 2061
TaqI	TCGA	7 cut(s)	813, 864, 1231, 1528, 1913, 2051, 2167
TatI	WGTACW	2 cut(s)	184, 759
TauI	GCSGC	4 cut(s)	674, 732, 809, 1634
TfiI	GAWTC	4 cut(s)	92, 861, 1064, 1982
TscAI	CASTG	4 cut(s)	180, 256, 1432, 2224
TseI	GCWGC	6 cut(s)	590, 593, 1010, 1517, 1619, 1670
TspDTI	ATGAA	7 cut(s)	105, 635, 1120, 1148, 1249, 1948, 2093
TspGWI	ACGGA	2 cut(s)	1920, 2201
TspMI	CCCGGG	3 cut(s)	715, 1499, 1813
TspRI	CASTG	4 cut(s)	180, 256, 1432, 2224
Van91I	CCANNNNNTGG	1 cut(s)	2233
VneI	GTGCAC	1 cut(s)	1863
VpaK11BI	GGWCC	6 cut(s)	651, 886, 959, 1213, 1451, 1564
XapI	RAATTY	1 cut(s)	1577
XbaI	TCTAGA	1 cut(s)	1445
XceI	RCATGY	3 cut(s)	1143, 1619, 2234
XcmI	CCANNNNNNNNNTGG	1 cut(s)	424
XmaI	CCCGGG	3 cut(s)	715, 1499, 1813
XmiI	GTMKAC	1 cut(s)	2051
XmnI	GAANNNNTTC	1 cut(s)	1534
XspI	CTAG	6 cut(s)	1014, 1434, 1446, 1628, 1761, 2302
Zsp2I	ATGCAT	2 cut(s)	124, 1141

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.