FvH4_1g08300

Belongs to the universal ribosomal protein uS3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001560
Physical Location & Seq
Reverse (-)
4393388 .. 4396060
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g08300.t1

Sequence Viewer

Length: 750 bp
ATGGCGACGGCTACACAAATGAGCAAGAAGCGAAAGTTCGTCGCTGACGGCGTTTTCTTCGCCGAGCTCAACGAGCTTCTGATGAGGGAGCTCGCCGAAGACGGCTACTCCGGCGCTGAGGTTAGGGTTACGCCGATGCGCACTGAGATCATAATCAGGGCTACCAGGACTCAAAACGTGCTCGGAGAGAAGGGGAGGAGGATTAGGGAGCTGACCTCAGTTGTGCAGAAGAGGTTCAAGTTCCCTGAGAAAGGTGTCGAGCTTTATGCTGAGAAGGTCGCCAACAGAGGACTTTGCTCTACTGCTCAGGCTGAGTCTCTCCGATACAAGCTTCTCGGAGGCCTACCTGTTCGCAGGGCTTGCTATGGTGTTTTAAGATTTGTCATGGAAAGTGGCGCAAAGGGTTGTGAGGTGATTGTTAGTGGAAAGCTGAGGGCACAGAGAGCCAAGGCCATGAAGTTCAAGGATGGCTACATGATTTCTTCTGGTCAGCCAGTGAATGAGTACATTGACTCAGCTGTGAGACATGTTCTCCTCAGACAGGGTGTTCTTGGTATCAAGGTCAAGATTATGCTCGACTGGGATCCCAAGGGCAAGCAAGGACCCATGACTCCTCTACCTGATCTGGTTACAATCCACCAACCAAAGGATGATGAGTATGTCAGGCGGGCACCAGAAATTGAAGCAGCGCCTCTTTTGGCCCCAACTGATATTGACATTCCAGCCATGCCAGCCCTCCCAGTGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
KH_2 Ribosomal_S3_C

Protein Analysis

250

Amino Acids

27.67

Weight (kDa)

9.61

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_2 PF07650 22 - 96 1.3e-13 KH domain
Ribosomal_S3_C PF00189 108 - 190 3.7e-26 Ribosomal protein S3, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 140
AccB1I GGYRCC 1 cut(s) 670
AciI CCGC 1 cut(s) 667
AclWI GGATC 2 cut(s) 578, 591
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 4 cut(s) 251, 541, 646, 742
AflIII ACRYGT 1 cut(s) 526
AgsI TTSAA 3 cut(s) 238, 463, 683
AjnI CCWGG 1 cut(s) 164
AluBI AGCT 8 cut(s) 67, 76, 91, 211, 262, 331, 430, 518
AluI AGCT 8 cut(s) 67, 76, 91, 211, 262, 331, 430, 518
Alw21I GWGCWC 3 cut(s) 69, 93, 183
Alw26I GTCTC 2 cut(s) 321, 517
AlwI GGATC 2 cut(s) 578, 591
AoxI GGCC 3 cut(s) 340, 450, 699
ApeKI GCWGC 1 cut(s) 686
Asp700I GAANNNNTTC 1 cut(s) 233
AspLEI GCGC 4 cut(s) 116, 141, 398, 691
AspS9I GGNCC 2 cut(s) 602, 700
AsuHPI GGTGA 1 cut(s) 424
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 2 cut(s) 439, 673
BamHI GGATCC 1 cut(s) 583
BanI GGYRCC 1 cut(s) 670
BanII GRGCYC 2 cut(s) 69, 93
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 3 cut(s) 69, 93, 183
BbvCI CCTCAGC 2 cut(s) 117, 431
BbvI GCAGC 1 cut(s) 698
BccI CCATC 1 cut(s) 461
BceAI ACGGC 3 cut(s) 24, 64, 118
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 1 cut(s) 166
BcoDI GTCTC 2 cut(s) 321, 517
BfoI RGCGCY 2 cut(s) 117, 692
BisI GCNGC 1 cut(s) 687
BlsI GCNGC 1 cut(s) 688
Bme1390I CCNGG 1 cut(s) 166
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 2 cut(s) 602, 700
BmiI GGNNCC 4 cut(s) 585, 604, 672, 702
BmrFI CCNGG 1 cut(s) 166
BmrI ACTGGG 2 cut(s) 589, 734
BmsI GCATC 1 cut(s) 126
BmuI ACTGGG 2 cut(s) 589, 734
BpiI GAAGAC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 200, 232
Bpu10I CCTNAGC 3 cut(s) 117, 306, 431
BsaBI GATNNNNATC 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 447, 588
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 516, 546
Bsc4I CCNNNNNNNGG 4 cut(s) 251, 541, 646, 742
Bse1I ACTGG 3 cut(s) 494, 584, 740
Bse8I GATNNNNATC 1 cut(s) 152
BseBI CCWGG 1 cut(s) 166
BseDI CCNNGG 2 cut(s) 447, 588
BseGI GGATG 2 cut(s) 472, 655
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 4 cut(s) 251, 541, 646, 742
BseNI ACTGG 3 cut(s) 494, 584, 740
BseRI GAGGAG 3 cut(s) 211, 524, 603
BseSI GKGCMC 2 cut(s) 439, 673
BseXI GCAGC 1 cut(s) 698
BsgI GTGCAG 1 cut(s) 245
BshFI GGCC 3 cut(s) 342, 452, 701
BshNI GGYRCC 1 cut(s) 670
BsiHKAI GWGCWC 3 cut(s) 69, 93, 183
BsiSI CCGG 1 cut(s) 111
BslI CCNNNNNNNGG 4 cut(s) 251, 541, 646, 742
BsmAI GTCTC 2 cut(s) 321, 517
BsnI GGCC 3 cut(s) 342, 452, 701
Bsp1286I GDGCHC 5 cut(s) 69, 93, 183, 439, 673
Bsp143I GATC 3 cut(s) 147, 583, 622
BspACI CCGC 1 cut(s) 667
BspANI GGCC 3 cut(s) 342, 452, 701
BspLI GGNNCC 4 cut(s) 585, 604, 672, 702
BspPI GGATC 2 cut(s) 578, 591
BspT107I GGYRCC 1 cut(s) 670
BsrI ACTGG 3 cut(s) 494, 584, 740
BssECI CCNNGG 2 cut(s) 447, 588
BssMI GATC 3 cut(s) 147, 583, 622
BssT1I CCWWGG 2 cut(s) 447, 588
Bst2UI CCWGG 1 cut(s) 166
Bst6I CTCTTC 1 cut(s) 224
BstAPI GCANNNNNTGC 1 cut(s) 360
BstC8I GCNNGC 5 cut(s) 93, 361, 596, 669, 732
BstENI CCTNNNNNAGG 2 cut(s) 249, 539
BstF5I GGATG 2 cut(s) 472, 655
BstH2I RGCGCY 2 cut(s) 117, 692
BstHHI GCGC 4 cut(s) 116, 141, 398, 691
BstKTI GATC 3 cut(s) 150, 586, 625
BstMAI GTCTC 2 cut(s) 321, 517
BstMBI GATC 3 cut(s) 147, 583, 622
BstMWI GCNNNNNNNGC 5 cut(s) 73, 111, 360, 443, 731
BstNI CCWGG 1 cut(s) 166
BstNSI RCATGY 1 cut(s) 530
BstSCI CCNGG 1 cut(s) 164
BstSLI GKGCMC 2 cut(s) 439, 673
BstV1I GCAGC 1 cut(s) 698
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BsuRI GGCC 3 cut(s) 342, 452, 701
BtsCI GGATG 2 cut(s) 472, 655
BtsIMutI CAGTG 3 cut(s) 141, 501, 747
Cac8I GCNNGC 5 cut(s) 93, 361, 596, 669, 732
CfoI GCGC 4 cut(s) 116, 141, 398, 691
Cfr13I GGNCC 2 cut(s) 602, 700
Csp6I GTAC 1 cut(s) 505
CviAII CATG 6 cut(s) 385, 454, 475, 527, 607, 727
CviQI GTAC 1 cut(s) 505
DpnI GATC 3 cut(s) 149, 585, 624
DpnII GATC 3 cut(s) 147, 583, 622
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
Ecl136II GAGCTC 2 cut(s) 67, 91
Eco130I CCWWGG 2 cut(s) 447, 588
Eco147I AGGCCT 1 cut(s) 342
Eco24I GRGCYC 2 cut(s) 69, 93
Eco47I GGWCC 1 cut(s) 602
Eco53kI GAGCTC 2 cut(s) 67, 91
EcoICRI GAGCTC 2 cut(s) 67, 91
EcoNI CCTNNNNNAGG 2 cut(s) 249, 539
EcoO109I RGGNCCY 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 164
EcoT14I CCWWGG 2 cut(s) 447, 588
EcoT38I GRGCYC 2 cut(s) 69, 93
ErhI CCWWGG 2 cut(s) 447, 588
FaeI CATG 6 cut(s) 388, 457, 478, 530, 610, 730
FatI CATG 6 cut(s) 384, 453, 474, 526, 606, 726
FauI CCCGC 1 cut(s) 660
Fnu4HI GCNGC 1 cut(s) 687
FokI GGATG 2 cut(s) 479, 662
FriOI GRGCYC 2 cut(s) 69, 93
Fsp4HI GCNGC 1 cut(s) 687
FspAI RTGCGCAY 1 cut(s) 140
FspI TGCGCA 1 cut(s) 140
GlaI GCGC 4 cut(s) 115, 140, 397, 690
GluI GCNGC 1 cut(s) 687
HaeII RGCGCY 2 cut(s) 117, 692
HaeIII GGCC 3 cut(s) 342, 452, 701
HapII CCGG 1 cut(s) 111
HhaI GCGC 4 cut(s) 116, 141, 398, 691
Hin1II CATG 6 cut(s) 388, 457, 478, 530, 610, 730
Hin6I GCGC 4 cut(s) 114, 139, 396, 689
HinP1I GCGC 4 cut(s) 114, 139, 396, 689
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 4 cut(s) 169, 314, 512, 610
HpaII CCGG 1 cut(s) 111
HphI GGTGA 1 cut(s) 424
Hpy188I TCNGA 5 cut(s) 81, 185, 323, 338, 539
Hpy188III TCNNGA 1 cut(s) 565
Hpy99I CGWCG 2 cut(s) 10, 44
HpyAV CCTTC 2 cut(s) 184, 268
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 1 cut(s) 226
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 111, 360, 443, 731
HpySE526I ACGT 1 cut(s) 177
Hsp92II CATG 6 cut(s) 388, 457, 478, 530, 610, 730
HspAI GCGC 4 cut(s) 114, 139, 396, 689
Kzo9I GATC 3 cut(s) 147, 583, 622
LmnI GCTCC 2 cut(s) 88, 208
Lsp1109I GCAGC 1 cut(s) 698
LweI GCATC 1 cut(s) 126
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 2 cut(s) 127, 628
MalI GATC 3 cut(s) 149, 585, 624
MboI GATC 3 cut(s) 147, 583, 622
MboII GAAGA 4 cut(s) 49, 110, 241, 474
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 5 cut(s) 69, 93, 183, 439, 673
MluCI AATT 1 cut(s) 678
MlyI GAGTC 4 cut(s) 163, 323, 506, 604
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 2 cut(s) 374, 748
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 1 cut(s) 166
MvaI CCWGG 1 cut(s) 166
MwoI GCNNNNNNNGC 5 cut(s) 73, 111, 360, 443, 731
NdeII GATC 3 cut(s) 147, 583, 622
NlaIII CATG 6 cut(s) 388, 457, 478, 530, 610, 730
NlaIV GGNNCC 4 cut(s) 585, 604, 672, 702
NmeAIII GCCGAG 1 cut(s) 88
NsbI TGCGCA 1 cut(s) 140
NspI RCATGY 1 cut(s) 530
PceI AGGCCT 1 cut(s) 342
PciI ACATGT 1 cut(s) 526
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PdmI GAANNNNTTC 1 cut(s) 233
PkrI GCNGC 1 cut(s) 688
PleI GAGTC 4 cut(s) 163, 322, 506, 604
PpsI GAGTC 4 cut(s) 163, 322, 506, 604
PpuMI RGGWCCY 1 cut(s) 602
PscI ACATGT 1 cut(s) 526
Psp124BI GAGCTC 2 cut(s) 69, 93
Psp5II RGGWCCY 1 cut(s) 602
Psp6I CCWGG 1 cut(s) 164
PspGI CCWGG 1 cut(s) 164
PspN4I GGNNCC 4 cut(s) 585, 604, 672, 702
PspPI GGNCC 2 cut(s) 602, 700
PspPPI RGGWCCY 1 cut(s) 602
PsuI RGATCY 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SacI GAGCTC 2 cut(s) 69, 93
SaqAI TTAA 2 cut(s) 374, 748
SatI GCNGC 1 cut(s) 687
Sau3AI GATC 3 cut(s) 147, 583, 622
Sau96I GGNCC 2 cut(s) 602, 700
SchI GAGTC 4 cut(s) 163, 323, 506, 604
ScrFI CCNGG 1 cut(s) 166
SduI GDGCHC 5 cut(s) 69, 93, 183, 439, 673
SfaNI GCATC 1 cut(s) 126
SinI GGWCC 1 cut(s) 602
Sse9I AATT 1 cut(s) 678
SseBI AGGCCT 1 cut(s) 342
SsiI CCGC 1 cut(s) 667
SstI GAGCTC 2 cut(s) 69, 93
StuI AGGCCT 1 cut(s) 342
StyD4I CCNGG 1 cut(s) 164
StyI CCWWGG 2 cut(s) 447, 588
TaiI ACGT 1 cut(s) 180
TaqI TCGA 2 cut(s) 258, 576
TasI AATT 1 cut(s) 678
TatI WGTACW 1 cut(s) 504
Tru1I TTAA 2 cut(s) 374, 748
Tru9I TTAA 2 cut(s) 374, 748
TscAI CASTG 3 cut(s) 148, 501, 747
TseI GCWGC 1 cut(s) 686
TspDTI ATGAA 1 cut(s) 470
TspRI CASTG 3 cut(s) 148, 501, 747
VpaK11BI GGWCC 1 cut(s) 602
XagI CCTNNNNNAGG 2 cut(s) 249, 539
XceI RCATGY 1 cut(s) 530
XmnI GAANNNNTTC 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.