Rroxscaffold_7G00157430

Belongs to the universal ribosomal protein uS3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001560
Physical Location & Seq
Forward (+)
1250433 .. 1252504
2072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00157430.1

Sequence Viewer

Length: 732 bp
ATGGCGACTCAGGTTCAGATGAGCAAGAAACGAAAGTTCGTAGCCGACGGAGTTTTCTTTGCTGAACTGAATGAGGTTCTGACCAGAGAGCTCGCCGAGGATGGTTACTCCGGTGTCGAAGTGAGGGTCACCCCCGTCCGCACTGAGATCATCATTAGAGCCACTCGCACCCAGAATGTTCTCGGTGAGAAGGGGAGGAGGATTAGAGAGCTAACCTCTGTAGTACAGAAGAGGTTCAAGTTTCCAGAGGGAAGTGTAGAGCTTTATGCCGAGAAGGTCAACAATAGGGGACTTTGTGCCATTGCGCAGGCCGAGTCTCTCCGGTACAAACTCCTTGGAGGGCTTGCCGTTCGCAGGGCATGCTATGGTGTTTTGAGATTTGTCATGGAGAGTGGCGCAAAGGGATGTGAGGTTATTGTAAGTGGAAAGCTCAGGGCACAACGTGCCAAGTCGATGAAGTTTAAGGATGGCTATATGATATCCTCCGGTCAACCTGTTAATGAGTACATTGACTCCGCTGTTAGGCACGTTCTTCTTAGACAGGGTGTGCTCGGTATTAAGGTGAAGATCATGCTTGACTGGGATCCCAAGGGGAAGCAGGGACCAATGACCCCTCTCCCTGATTTGGTGACCATCCATACTCCCAAGGAAGAACTCTATACTCCAGCTCCTCCAGTGGCAGCAATCAACATAGATGCACTTCCATCTCCTGTTCCTGTTCCAGTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
KH_2 Ribosomal_S3_C

Protein Analysis

243

Amino Acids

26.88

Weight (kDa)

9.7

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_2 PF07650 22 - 96 1.4e-14 KH domain
Ribosomal_S3_C PF00189 108 - 190 1.2e-26 Ribosomal protein S3, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 306
AciI CCGC 2 cut(s) 139, 516
AclWI GGATC 2 cut(s) 578, 591
AdeI CACNNNGTG 1 cut(s) 443
AfaI GTAC 3 cut(s) 225, 326, 506
AfiI CCNNNNNNNGG 3 cut(s) 354, 522, 625
AgsI TTSAA 1 cut(s) 238
AluBI AGCT 5 cut(s) 91, 211, 262, 430, 668
AluI AGCT 5 cut(s) 91, 211, 262, 430, 668
Alw21I GWGCWC 2 cut(s) 93, 552
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 2 cut(s) 578, 591
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 1 cut(s) 680
ArsI GACNNNNNNTTYG 2 cut(s) 111, 143
Asp700I GAANNNNTTC 1 cut(s) 233
AspLEI GCGC 2 cut(s) 307, 398
AspS9I GGNCC 1 cut(s) 602
AsuHPI GGTGA 4 cut(s) 121, 197, 574, 640
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 1 cut(s) 439
BamHI GGATCC 1 cut(s) 583
BanII GRGCYC 1 cut(s) 93
Bbv12I GWGCWC 2 cut(s) 93, 552
BbvI GCAGC 1 cut(s) 692
BccI CCATC 4 cut(s) 95, 461, 641, 712
BceAI ACGGC 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 321
BfmI CTRYAG 1 cut(s) 219
BisI GCNGC 1 cut(s) 681
BlsI GCNGC 1 cut(s) 682
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 1 cut(s) 602
BmiI GGNNCC 2 cut(s) 585, 603
BmrI ACTGGG 1 cut(s) 589
BmsI GCATC 1 cut(s) 685
BmuI ACTGGG 1 cut(s) 589
BplI GAGNNNNNCTC 2 cut(s) 200, 232
BpmI CTGGAG 2 cut(s) 648, 657
Bpu10I CCTNAGC 1 cut(s) 431
BsaJI CCNNGG 4 cut(s) 96, 334, 588, 645
BsaWI WCCGGW 3 cut(s) 110, 321, 485
BsaXI ACNNNNNCTCC 4 cut(s) 497, 527, 652, 682
Bsc4I CCNNNNNNNGG 3 cut(s) 354, 522, 625
Bse1I ACTGG 3 cut(s) 584, 674, 722
Bse3DI GCAATG 1 cut(s) 300
BseDI CCNNGG 4 cut(s) 96, 334, 588, 645
BseGI GGATG 4 cut(s) 106, 410, 472, 633
BseLI CCNNNNNNNGG 3 cut(s) 354, 522, 625
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 3 cut(s) 23, 135, 445
BseNI ACTGG 3 cut(s) 584, 674, 722
BseRI GAGGAG 2 cut(s) 211, 660
BseSI GKGCMC 1 cut(s) 439
BseXI GCAGC 1 cut(s) 692
BshFI GGCC 1 cut(s) 311
BsiHKAI GWGCWC 2 cut(s) 93, 552
BsiSI CCGG 3 cut(s) 111, 322, 486
BslFI GGGAC 2 cut(s) 303, 615
BslI CCNNNNNNNGG 3 cut(s) 354, 522, 625
BsmAI GTCTC 1 cut(s) 321
BsmFI GGGAC 2 cut(s) 303, 615
BsnI GGCC 1 cut(s) 311
Bsp1286I GDGCHC 3 cut(s) 93, 439, 552
Bsp143I GATC 3 cut(s) 147, 567, 583
BspACI CCGC 2 cut(s) 139, 516
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 3 cut(s) 22, 136, 444
BspLI GGNNCC 2 cut(s) 585, 603
BspPI GGATC 2 cut(s) 578, 591
BsrDI GCAATG 1 cut(s) 300
BsrI ACTGG 3 cut(s) 584, 674, 722
BssECI CCNNGG 4 cut(s) 96, 334, 588, 645
BssMI GATC 3 cut(s) 147, 567, 583
BssT1I CCWWGG 3 cut(s) 334, 588, 645
Bst6I CTCTTC 1 cut(s) 224
BstAPI GCANNNNNTGC 2 cut(s) 360, 443
BstC8I GCNNGC 4 cut(s) 93, 309, 345, 361
BstDEI CTNAG 4 cut(s) 9, 144, 431, 536
BstEII GGTNACC 2 cut(s) 127, 628
BstF5I GGATG 4 cut(s) 106, 410, 472, 633
BstHHI GCGC 2 cut(s) 307, 398
BstKTI GATC 3 cut(s) 150, 570, 586
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 3 cut(s) 147, 567, 583
BstMWI GCNNNNNNNGC 2 cut(s) 360, 443
BstNSI RCATGY 1 cut(s) 363
BstPI GGTNACC 2 cut(s) 127, 628
BstSFI CTRYAG 1 cut(s) 219
BstSLI GKGCMC 1 cut(s) 439
BstV1I GCAGC 1 cut(s) 692
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BsuRI GGCC 1 cut(s) 311
BtsCI GGATG 4 cut(s) 106, 410, 472, 633
BtsIMutI CAGTG 2 cut(s) 141, 681
Cac8I GCNNGC 4 cut(s) 93, 309, 345, 361
CfoI GCGC 2 cut(s) 307, 398
Cfr13I GGNCC 1 cut(s) 602
Csp6I GTAC 3 cut(s) 224, 325, 505
CviAII CATG 3 cut(s) 360, 385, 571
CviQI GTAC 3 cut(s) 224, 325, 505
DdeI CTNAG 4 cut(s) 9, 144, 431, 536
DpnI GATC 3 cut(s) 149, 569, 585
DpnII GATC 3 cut(s) 147, 567, 583
DraIII CACNNNGTG 1 cut(s) 443
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
Ecl136II GAGCTC 1 cut(s) 91
Eco130I CCWWGG 3 cut(s) 334, 588, 645
Eco24I GRGCYC 1 cut(s) 93
Eco32I GATATC 1 cut(s) 480
Eco47I GGWCC 1 cut(s) 602
Eco53kI GAGCTC 1 cut(s) 91
Eco91I GGTNACC 2 cut(s) 127, 628
EcoICRI GAGCTC 1 cut(s) 91
EcoO65I GGTNACC 2 cut(s) 127, 628
EcoRV GATATC 1 cut(s) 480
EcoT14I CCWWGG 3 cut(s) 334, 588, 645
EcoT38I GRGCYC 1 cut(s) 93
ErhI CCWWGG 3 cut(s) 334, 588, 645
FaeI CATG 3 cut(s) 363, 388, 574
FaqI GGGAC 2 cut(s) 303, 615
FatI CATG 3 cut(s) 359, 384, 570
Fnu4HI GCNGC 1 cut(s) 681
FokI GGATG 4 cut(s) 113, 417, 479, 620
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 1 cut(s) 681
FspI TGCGCA 1 cut(s) 306
GlaI GCGC 2 cut(s) 306, 397
GluI GCNGC 1 cut(s) 681
GsuI CTGGAG 2 cut(s) 648, 657
HaeIII GGCC 1 cut(s) 311
HapII CCGG 3 cut(s) 111, 322, 486
HhaI GCGC 2 cut(s) 307, 398
Hin1II CATG 3 cut(s) 363, 388, 574
Hin6I GCGC 2 cut(s) 305, 396
HinP1I GCGC 2 cut(s) 305, 396
HincII GTYRAC 2 cut(s) 280, 491
HindII GTYRAC 2 cut(s) 280, 491
HinfI GANTC 3 cut(s) 7, 314, 512
HpaII CCGG 3 cut(s) 111, 322, 486
HphI GGTGA 4 cut(s) 121, 197, 574, 640
Hpy166II GTNNAC 2 cut(s) 280, 491
Hpy188I TCNGA 2 cut(s) 18, 81
Hpy188III TCNNGA 1 cut(s) 245
Hpy8I GTNNAC 2 cut(s) 280, 491
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 2 cut(s) 184, 268
HpyCH4IV ACGT 2 cut(s) 442, 528
HpyCH4V TGCA 1 cut(s) 698
HpyF10VI GCNNNNNNNGC 2 cut(s) 360, 443
HpyF3I CTNAG 4 cut(s) 9, 144, 431, 536
HpySE526I ACGT 2 cut(s) 442, 528
Hsp92II CATG 3 cut(s) 363, 388, 574
HspAI GCGC 2 cut(s) 305, 396
Kzo9I GATC 3 cut(s) 147, 567, 583
LmnI GCTCC 1 cut(s) 673
Lsp1109I GCAGC 1 cut(s) 692
LweI GCATC 1 cut(s) 685
MaeII ACGT 2 cut(s) 442, 528
MaeIII GTNAC 3 cut(s) 104, 127, 628
MalI GATC 3 cut(s) 149, 569, 585
MboI GATC 3 cut(s) 147, 567, 583
MboII GAAGA 4 cut(s) 241, 524, 577, 662
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 3 cut(s) 93, 439, 552
MlyI GAGTC 2 cut(s) 323, 506
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 4 cut(s) 462, 498, 558, 730
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 3 cut(s) 111, 322, 486
MwoI GCNNNNNNNGC 2 cut(s) 360, 443
NdeII GATC 3 cut(s) 147, 567, 583
NlaIII CATG 3 cut(s) 363, 388, 574
NlaIV GGNNCC 2 cut(s) 585, 603
NmeAIII GCCGAG 3 cut(s) 121, 295, 337
NmuCI GTSAC 2 cut(s) 127, 628
NsbI TGCGCA 1 cut(s) 306
NspI RCATGY 1 cut(s) 363
PaeI GCATGC 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 233
PkrI GCNGC 1 cut(s) 682
PleI GAGTC 2 cut(s) 322, 506
PpsI GAGTC 2 cut(s) 322, 506
Psp124BI GAGCTC 1 cut(s) 93
PspEI GGTNACC 2 cut(s) 127, 628
PspN4I GGNNCC 2 cut(s) 585, 603
PspPI GGNCC 1 cut(s) 602
PsuI RGATCY 1 cut(s) 583
RsaI GTAC 3 cut(s) 225, 326, 506
RsaNI GTAC 3 cut(s) 224, 325, 505
SacI GAGCTC 1 cut(s) 93
SaqAI TTAA 4 cut(s) 462, 498, 558, 730
SatI GCNGC 1 cut(s) 681
Sau3AI GATC 3 cut(s) 147, 567, 583
Sau96I GGNCC 1 cut(s) 602
SchI GAGTC 2 cut(s) 323, 506
SduI GDGCHC 3 cut(s) 93, 439, 552
SfaNI GCATC 1 cut(s) 685
SfcI CTRYAG 1 cut(s) 219
SinI GGWCC 1 cut(s) 602
SphI GCATGC 1 cut(s) 363
SsiI CCGC 2 cut(s) 139, 516
SstI GAGCTC 1 cut(s) 93
StyI CCWWGG 3 cut(s) 334, 588, 645
TaiI ACGT 2 cut(s) 445, 531
TaqI TCGA 2 cut(s) 117, 452
TatI WGTACW 2 cut(s) 223, 504
Tru1I TTAA 4 cut(s) 462, 498, 558, 730
Tru9I TTAA 4 cut(s) 462, 498, 558, 730
TscAI CASTG 2 cut(s) 148, 681
TseFI GTSAC 2 cut(s) 127, 628
TseI GCWGC 1 cut(s) 680
Tsp45I GTSAC 2 cut(s) 127, 628
TspDTI ATGAA 1 cut(s) 470
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 2 cut(s) 148, 681
VpaK11BI GGWCC 1 cut(s) 602
XceI RCATGY 1 cut(s) 363
XmnI GAANNNNTTC 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.