Rh2BG641500

Belongs to the universal ribosomal protein uS3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001560
Physical Location & Seq
Forward (+)
86055485 .. 86056165
681 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG641500.1

Sequence Viewer

Length: 681 bp
ATGGCGACCCAGATGAGCAAGAAGCGAAAGTTCGTCGCTGACGGTGTTTTCTTCGCCGAGCTCAACGAGCTTCTGATGAGAGAGCTCGCCGAGGACGGCTACTCCGGCGCTGAGGTTAGGGTTACGCCGATGCGCACTGAGATCATAATCAGGGCGACTCGGACTCAAAACGTGCTCGGAGAGAAGGGAAGGAGGATTAGGGAGCTGACCTCAGTTGTGCAGAAGAGGTTTAAGTTCCCGGAGAAAGGTGTGGAGCTTTATGCTGAGAAGGTAGCCAACAGAGGGCTCTGCTCTACTGCCCAGGCTGAGTCTCTTCGCTACAAGCTTCTCGGAGGCCTACCGGTTCGCAGGGCCTGCTATGGTGTTTTGAGATTTGTCATGGAAAGTGGTGCAAAGGGTTGTGAGGTGATTGTTAGTGGAAAGCTGAGGGCACAGAGAGCCAAGTCCATGAAGTTCAAGGATGGATACATGATTTCTTCTGGTCAGCCAGTCAATGAGTACATTGACGCAGCTGTGAGACATGTTCTCCTTAGACAGGTTGTTCTTGGTATTAAGGTCAAGATCATGCTCGACTGGGATCCCAAGGGCAAGCAAGGACCCATGACTCCTTTACCTGATCTTGTTACAATCCACCAACCAAAGGATGATGAGTTTGTCAGGCGGCCGGGCACCAGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
KH_2 Ribosomal_S3_C

Protein Analysis

226

Amino Acids

25.47

Weight (kDa)

9.97

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_2 PF07650 20 - 94 1.1e-13 KH domain
Ribosomal_S3_C PF00189 106 - 188 1.1e-24 Ribosomal protein S3, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 134
AccB1I GGYRCC 1 cut(s) 668
AciI CCGC 1 cut(s) 661
AclWI GGATC 2 cut(s) 572, 585
AcoI YGGCCR 1 cut(s) 662
AfaI GTAC 1 cut(s) 500
AfiI CCNNNNNNNGG 4 cut(s) 245, 282, 535, 640
AflIII ACRYGT 1 cut(s) 520
AgeI ACCGGT 1 cut(s) 340
AgsI TTSAA 1 cut(s) 457
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 8 cut(s) 61, 70, 85, 205, 256, 325, 424, 512
AluI AGCT 8 cut(s) 61, 70, 85, 205, 256, 325, 424, 512
Alw21I GWGCWC 3 cut(s) 63, 87, 177
Alw26I GTCTC 2 cut(s) 315, 511
AlwI GGATC 2 cut(s) 572, 585
AlwNI CAGNNNCTG 1 cut(s) 354
AoxI GGCC 3 cut(s) 334, 351, 662
ApeKI GCWGC 1 cut(s) 509
AsiGI ACCGGT 1 cut(s) 340
AspLEI GCGC 2 cut(s) 110, 135
AspS9I GGNCC 2 cut(s) 351, 596
AsuC2I CCSGG 2 cut(s) 239, 666
AsuHPI GGTGA 1 cut(s) 418
AvaII GGWCC 1 cut(s) 596
BaeGI GKGCMC 2 cut(s) 433, 671
BamHI GGATCC 1 cut(s) 577
BanI GGYRCC 1 cut(s) 668
BanII GRGCYC 3 cut(s) 63, 87, 288
Bbv12I GWGCWC 3 cut(s) 63, 87, 177
BbvCI CCTCAGC 2 cut(s) 111, 425
BbvI GCAGC 1 cut(s) 521
BccI CCATC 1 cut(s) 455
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 1 cut(s) 302
BciVI GTATCC 1 cut(s) 458
BcnI CCSGG 2 cut(s) 239, 666
BcoDI GTCTC 2 cut(s) 315, 511
BfoI RGCGCY 1 cut(s) 111
BfuI GTATCC 1 cut(s) 458
BisI GCNGC 2 cut(s) 510, 662
BlsI GCNGC 2 cut(s) 511, 663
Bme1390I CCNGG 3 cut(s) 239, 302, 666
Bme18I GGWCC 1 cut(s) 596
BmgT120I GGNCC 2 cut(s) 351, 596
BmiI GGNNCC 3 cut(s) 579, 598, 670
BmrFI CCNGG 3 cut(s) 239, 302, 666
BmrI ACTGGG 1 cut(s) 583
BmsI GCATC 1 cut(s) 120
BmuI ACTGGG 1 cut(s) 583
BplI GAGNNNNNCTC 2 cut(s) 194, 226
Bpu10I CCTNAGC 2 cut(s) 111, 425
BpuMI CCSGG 2 cut(s) 239, 666
BsaBI GATNNNNATC 1 cut(s) 146
BsaJI CCNNGG 3 cut(s) 90, 300, 582
BsaWI WCCGGW 1 cut(s) 340
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 510, 540
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 282, 535, 640
Bse118I RCCGGY 1 cut(s) 340
Bse1I ACTGG 2 cut(s) 488, 578
Bse8I GATNNNNATC 1 cut(s) 146
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 3 cut(s) 90, 300, 582
BseGI GGATG 2 cut(s) 466, 649
BseJI GATNNNNATC 1 cut(s) 146
BseLI CCNNNNNNNGG 4 cut(s) 245, 282, 535, 640
BseMII CTCAG 6 cut(s) 102, 129, 225, 255, 297, 416
BseNI ACTGG 2 cut(s) 488, 578
BseSI GKGCMC 2 cut(s) 433, 671
BseX3I CGGCCG 1 cut(s) 662
BseXI GCAGC 1 cut(s) 521
BsgI GTGCAG 1 cut(s) 239
Bsh1285I CGRYCG 1 cut(s) 665
BshFI GGCC 3 cut(s) 336, 353, 664
BshNI GGYRCC 1 cut(s) 668
BshTI ACCGGT 1 cut(s) 340
BsiEI CGRYCG 1 cut(s) 665
BsiHKAI GWGCWC 3 cut(s) 63, 87, 177
BsiSI CCGG 4 cut(s) 105, 239, 341, 665
BslI CCNNNNNNNGG 4 cut(s) 245, 282, 535, 640
BsmAI GTCTC 2 cut(s) 315, 511
BsnI GGCC 3 cut(s) 336, 353, 664
Bsp1286I GDGCHC 6 cut(s) 63, 87, 177, 288, 433, 671
Bsp143I GATC 4 cut(s) 141, 561, 577, 616
BspACI CCGC 1 cut(s) 661
BspANI GGCC 3 cut(s) 336, 353, 664
BspCNI CTCAG 6 cut(s) 103, 130, 224, 256, 298, 417
BspLI GGNNCC 3 cut(s) 579, 598, 670
BspPI GGATC 2 cut(s) 572, 585
BspT107I GGYRCC 1 cut(s) 668
BsrFI RCCGGY 1 cut(s) 340
BsrI ACTGG 2 cut(s) 488, 578
BssAI RCCGGY 1 cut(s) 340
BssECI CCNNGG 3 cut(s) 90, 300, 582
BssMI GATC 4 cut(s) 141, 561, 577, 616
BssT1I CCWWGG 1 cut(s) 582
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 1 cut(s) 44
Bst6I CTCTTC 2 cut(s) 218, 318
BstAPI GCANNNNNTGC 1 cut(s) 354
BstC8I GCNNGC 3 cut(s) 87, 355, 590
BstDEI CTNAG 7 cut(s) 111, 138, 211, 264, 306, 425, 530
BstENI CCTNNNNNAGG 1 cut(s) 533
BstF5I GGATG 2 cut(s) 466, 649
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 2 cut(s) 110, 135
BstKTI GATC 4 cut(s) 144, 564, 580, 619
BstMAI GTCTC 2 cut(s) 315, 511
BstMBI GATC 4 cut(s) 141, 561, 577, 616
BstMCI CGRYCG 1 cut(s) 665
BstMWI GCNNNNNNNGC 4 cut(s) 67, 105, 354, 437
BstNI CCWGG 1 cut(s) 302
BstNSI RCATGY 1 cut(s) 524
BstSCI CCNGG 3 cut(s) 237, 300, 664
BstSLI GKGCMC 2 cut(s) 433, 671
BstV1I GCAGC 1 cut(s) 521
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BstZI CGGCCG 1 cut(s) 662
BsuI GTATCC 1 cut(s) 458
BsuRI GGCC 3 cut(s) 336, 353, 664
BtsCI GGATG 2 cut(s) 466, 649
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 3 cut(s) 87, 355, 590
CaiI CAGNNNCTG 1 cut(s) 354
CfoI GCGC 2 cut(s) 110, 135
Cfr10I RCCGGY 1 cut(s) 340
Cfr13I GGNCC 2 cut(s) 351, 596
CseI GACGC 1 cut(s) 515
Csp6I GTAC 1 cut(s) 499
CspAI ACCGGT 1 cut(s) 340
CviAII CATG 6 cut(s) 379, 448, 469, 521, 565, 601
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 7 cut(s) 111, 138, 211, 264, 306, 425, 530
DpnI GATC 4 cut(s) 143, 563, 579, 618
DpnII GATC 4 cut(s) 141, 561, 577, 616
EaeI YGGCCR 1 cut(s) 662
EagI CGGCCG 1 cut(s) 662
Eam1104I CTCTTC 2 cut(s) 218, 318
EarI CTCTTC 2 cut(s) 218, 318
Ecl136II GAGCTC 2 cut(s) 61, 85
EclXI CGGCCG 1 cut(s) 662
Eco130I CCWWGG 1 cut(s) 582
Eco147I AGGCCT 1 cut(s) 336
Eco24I GRGCYC 3 cut(s) 63, 87, 288
Eco47I GGWCC 1 cut(s) 596
Eco52I CGGCCG 1 cut(s) 662
Eco53kI GAGCTC 2 cut(s) 61, 85
EcoICRI GAGCTC 2 cut(s) 61, 85
EcoNI CCTNNNNNAGG 1 cut(s) 533
EcoO109I RGGNCCY 2 cut(s) 351, 596
EcoRII CCWGG 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 582
EcoT38I GRGCYC 3 cut(s) 63, 87, 288
ErhI CCWWGG 1 cut(s) 582
FaeI CATG 6 cut(s) 382, 451, 472, 524, 568, 604
FaiI YATR 9 cut(s) 146, 261, 360, 380, 449, 470, 522, 566, 602
FatI CATG 6 cut(s) 378, 447, 468, 520, 564, 600
Fnu4HI GCNGC 2 cut(s) 510, 662
FokI GGATG 2 cut(s) 473, 656
FriOI GRGCYC 3 cut(s) 63, 87, 288
Fsp4HI GCNGC 2 cut(s) 510, 662
FspAI RTGCGCAY 1 cut(s) 134
FspI TGCGCA 1 cut(s) 134
GlaI GCGC 2 cut(s) 109, 134
GluI GCNGC 2 cut(s) 510, 662
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 3 cut(s) 336, 353, 664
HapII CCGG 4 cut(s) 105, 239, 341, 665
HgaI GACGC 1 cut(s) 515
HhaI GCGC 2 cut(s) 110, 135
Hin1II CATG 6 cut(s) 382, 451, 472, 524, 568, 604
Hin6I GCGC 2 cut(s) 108, 133
HinP1I GCGC 2 cut(s) 108, 133
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 4 cut(s) 157, 163, 308, 604
HpaII CCGG 4 cut(s) 105, 239, 341, 665
HphI GGTGA 1 cut(s) 418
Hpy188I TCNGA 4 cut(s) 75, 162, 179, 332
Hpy188III TCNNGA 1 cut(s) 559
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 3 cut(s) 178, 183, 262
HpyCH4III ACNGT 1 cut(s) 44
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 2 cut(s) 220, 392
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 105, 354, 437
HpyF3I CTNAG 7 cut(s) 111, 138, 211, 264, 306, 425, 530
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 6 cut(s) 382, 451, 472, 524, 568, 604
HspAI GCGC 2 cut(s) 108, 133
Kzo9I GATC 4 cut(s) 141, 561, 577, 616
LmnI GCTCC 2 cut(s) 202, 253
Lsp1109I GCAGC 1 cut(s) 521
LweI GCATC 1 cut(s) 120
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 121, 622
MalI GATC 4 cut(s) 143, 563, 579, 618
MboI GATC 4 cut(s) 141, 561, 577, 616
MboII GAAGA 4 cut(s) 43, 235, 305, 468
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 6 cut(s) 63, 87, 177, 288, 433, 671
MluCI AATT 1 cut(s) 676
MlyI GAGTC 4 cut(s) 151, 157, 317, 598
MnlI CCTC 9 cut(s) 85, 106, 186, 219, 220, 275, 326, 397, 420
MseI TTAA 2 cut(s) 231, 552
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 4 cut(s) 105, 239, 341, 665
MspR9I CCNGG 3 cut(s) 239, 302, 666
MvaI CCWGG 1 cut(s) 302
MwoI GCNNNNNNNGC 4 cut(s) 67, 105, 354, 437
NciI CCSGG 2 cut(s) 239, 666
NdeII GATC 4 cut(s) 141, 561, 577, 616
NlaIII CATG 6 cut(s) 382, 451, 472, 524, 568, 604
NlaIV GGNNCC 3 cut(s) 579, 598, 670
NmeAIII GCCGAG 2 cut(s) 82, 115
NsbI TGCGCA 1 cut(s) 134
NspI RCATGY 1 cut(s) 524
PceI AGGCCT 1 cut(s) 336
PciI ACATGT 1 cut(s) 520
PfoI TCCNGGA 1 cut(s) 237
PinAI ACCGGT 1 cut(s) 340
PkrI GCNGC 2 cut(s) 511, 663
PleI GAGTC 4 cut(s) 151, 157, 316, 598
PpsI GAGTC 4 cut(s) 151, 157, 316, 598
PpuMI RGGWCCY 1 cut(s) 596
PscI ACATGT 1 cut(s) 520
Psp124BI GAGCTC 2 cut(s) 63, 87
Psp5II RGGWCCY 1 cut(s) 596
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 3 cut(s) 579, 598, 670
PspPI GGNCC 2 cut(s) 351, 596
PspPPI RGGWCCY 1 cut(s) 596
PstNI CAGNNNCTG 1 cut(s) 354
PsuI RGATCY 1 cut(s) 577
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
SacI GAGCTC 2 cut(s) 63, 87
SaqAI TTAA 2 cut(s) 231, 552
SatI GCNGC 2 cut(s) 510, 662
Sau3AI GATC 4 cut(s) 141, 561, 577, 616
Sau96I GGNCC 2 cut(s) 351, 596
SchI GAGTC 4 cut(s) 151, 157, 317, 598
ScrFI CCNGG 3 cut(s) 239, 302, 666
SduI GDGCHC 6 cut(s) 63, 87, 177, 288, 433, 671
SfaNI GCATC 1 cut(s) 120
SinI GGWCC 1 cut(s) 596
Sse9I AATT 1 cut(s) 676
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 1 cut(s) 661
SstI GAGCTC 2 cut(s) 63, 87
StuI AGGCCT 1 cut(s) 336
StyD4I CCNGG 3 cut(s) 237, 300, 664
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 1 cut(s) 44
TaiI ACGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 570
TasI AATT 1 cut(s) 676
TatI WGTACW 1 cut(s) 498
TauI GCSGC 1 cut(s) 664
Tru1I TTAA 2 cut(s) 231, 552
Tru9I TTAA 2 cut(s) 231, 552
TscAI CASTG 1 cut(s) 142
TseI GCWGC 1 cut(s) 509
TspDTI ATGAA 1 cut(s) 464
TspRI CASTG 1 cut(s) 142
VpaK11BI GGWCC 1 cut(s) 596
XagI CCTNNNNNAGG 1 cut(s) 533
XceI RCATGY 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.