Rh2DG090300

Belongs to the universal ribosomal protein uS3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001560
Physical Location & Seq
Reverse (-)
6963666 .. 6966382
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG090300.1

Sequence Viewer

Length: 669 bp
ATGAGGGAGCTCGCCGAGGACGGCTACTCCGGCGCTGAGGTTAGGGTTACGCCGATGCGCACTGAGATCATAATCAGGGCGACTCGGACTCAAAACGTGCTCGGAGAGAAGGGAAGGAGGATTAGGGAGCTGACCTCAGTTGTGCAGAAGAGGTTCAAGTTCCCGGAGAAAGGTGTGGAGCTTTATGCTGAGAAGGTAGCCAACAGAGGGCTCTGCTCTACTGCCCAGGCTGAGTCTCTTCGCTACAAGCTTCTCGGAGGCCTACCGGTTCGCAGGGCCTGCTATGGTGTTTTGAGATTTGTCATGGAAAGTGGTGCAAAGGGTTGTGAGGTGATTGTTAGTGGAAAGCTGAGGGCACAGAGAGCCAAGTCCATGAAGTTCAAGGACGGATACATGATTTCTTCTGGTCAGCCAGTCAATGAGTACATTGACGCAGCTGTGAGACATGTTCTCCTGAGACAGGGTGTTCTTGGTATTAAGGTCAAGATCATGCTCGACTGGGATCCCAAGGGAAAGCAAGGACCCATGACTCCTCTACCGGATCTTGTTACAATCCACCAACCAAAGGATGATGAGTTTGTCAGGCGGGCACCAGAAATTGAAGCAGCACCTATTTTGGCCCCAACTGATATTGAGGTCCCAGCCATCCCAGCCCTCCCAGTGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
KH_2 Ribosomal_S3_C

Protein Analysis

222

Amino Acids

24.61

Weight (kDa)

9.62

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_2 PF07650 3 - 69 2e-12 KH domain
Ribosomal_S3_C PF00189 81 - 163 1.6e-26 Ribosomal protein S3, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 59
AccB1I GGYRCC 1 cut(s) 589
AciI CCGC 1 cut(s) 586
AclWI GGATC 3 cut(s) 497, 510, 549
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 5 cut(s) 170, 207, 460, 565, 661
AflIII ACRYGT 1 cut(s) 445
AgeI ACCGGT 1 cut(s) 265
AgsI TTSAA 3 cut(s) 157, 382, 602
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 6 cut(s) 10, 130, 181, 250, 349, 437
AluI AGCT 6 cut(s) 10, 130, 181, 250, 349, 437
Alw21I GWGCWC 2 cut(s) 12, 102
Alw26I GTCTC 3 cut(s) 240, 436, 451
AlwI GGATC 3 cut(s) 497, 510, 549
AlwNI CAGNNNCTG 1 cut(s) 279
AoxI GGCC 3 cut(s) 259, 276, 618
ApeKI GCWGC 2 cut(s) 434, 605
AsiGI ACCGGT 1 cut(s) 265
Asp700I GAANNNNTTC 1 cut(s) 152
AspLEI GCGC 2 cut(s) 35, 60
AspS9I GGNCC 4 cut(s) 276, 521, 619, 637
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 343
AvaII GGWCC 2 cut(s) 521, 637
BaeGI GKGCMC 2 cut(s) 358, 592
BamHI GGATCC 1 cut(s) 502
BanI GGYRCC 1 cut(s) 589
BanII GRGCYC 2 cut(s) 12, 213
Bbv12I GWGCWC 2 cut(s) 12, 102
BbvCI CCTCAGC 2 cut(s) 36, 350
BbvI GCAGC 2 cut(s) 446, 617
BccI CCATC 1 cut(s) 653
BceAI ACGGC 1 cut(s) 37
BciT130I CCWGG 1 cut(s) 227
BciVI GTATCC 1 cut(s) 383
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 3 cut(s) 240, 436, 451
BfoI RGCGCY 1 cut(s) 36
BfuI GTATCC 1 cut(s) 383
BisI GCNGC 2 cut(s) 435, 606
BlsI GCNGC 2 cut(s) 436, 607
Bme1390I CCNGG 2 cut(s) 164, 227
Bme18I GGWCC 2 cut(s) 521, 637
BmgT120I GGNCC 4 cut(s) 276, 521, 619, 637
BmiI GGNNCC 5 cut(s) 504, 523, 591, 621, 639
BmrFI CCNGG 2 cut(s) 164, 227
BmrI ACTGGG 2 cut(s) 508, 653
BmsI GCATC 1 cut(s) 45
BmuI ACTGGG 2 cut(s) 508, 653
BplI GAGNNNNNCTC 2 cut(s) 119, 151
Bpu10I CCTNAGC 2 cut(s) 36, 350
BpuMI CCSGG 1 cut(s) 164
BsaBI GATNNNNATC 1 cut(s) 71
BsaJI CCNNGG 3 cut(s) 15, 225, 507
BsaWI WCCGGW 2 cut(s) 265, 538
BsaXI ACNNNNNCTCC 4 cut(s) 11, 41, 435, 465
Bsc4I CCNNNNNNNGG 5 cut(s) 170, 207, 460, 565, 661
Bse118I RCCGGY 1 cut(s) 265
Bse1I ACTGG 3 cut(s) 413, 503, 659
Bse8I GATNNNNATC 1 cut(s) 71
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 3 cut(s) 15, 225, 507
BseGI GGATG 2 cut(s) 574, 645
BseJI GATNNNNATC 1 cut(s) 71
BseLI CCNNNNNNNGG 5 cut(s) 170, 207, 460, 565, 661
BseMII CTCAG 7 cut(s) 27, 54, 150, 180, 222, 341, 446
BseNI ACTGG 3 cut(s) 413, 503, 659
BseRI GAGGAG 1 cut(s) 522
BseSI GKGCMC 2 cut(s) 358, 592
BseXI GCAGC 2 cut(s) 446, 617
BseYI CCCAGC 2 cut(s) 640, 649
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 3 cut(s) 261, 278, 620
BshNI GGYRCC 1 cut(s) 589
BshTI ACCGGT 1 cut(s) 265
BsiHKAI GWGCWC 2 cut(s) 12, 102
BsiSI CCGG 4 cut(s) 30, 164, 266, 539
BslFI GGGAC 1 cut(s) 623
BslI CCNNNNNNNGG 5 cut(s) 170, 207, 460, 565, 661
BsmAI GTCTC 3 cut(s) 240, 436, 451
BsmFI GGGAC 1 cut(s) 623
BsnI GGCC 3 cut(s) 261, 278, 620
Bsp1286I GDGCHC 5 cut(s) 12, 102, 213, 358, 592
Bsp143I GATC 4 cut(s) 66, 486, 502, 541
BspACI CCGC 1 cut(s) 586
BspANI GGCC 3 cut(s) 261, 278, 620
BspCNI CTCAG 7 cut(s) 28, 55, 149, 181, 223, 342, 447
BspLI GGNNCC 5 cut(s) 504, 523, 591, 621, 639
BspPI GGATC 3 cut(s) 497, 510, 549
BspT107I GGYRCC 1 cut(s) 589
BsrFI RCCGGY 1 cut(s) 265
BsrI ACTGG 3 cut(s) 413, 503, 659
BssAI RCCGGY 1 cut(s) 265
BssECI CCNNGG 3 cut(s) 15, 225, 507
BssMI GATC 4 cut(s) 66, 486, 502, 541
BssT1I CCWWGG 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 227
Bst6I CTCTTC 2 cut(s) 143, 243
BstAPI GCANNNNNTGC 1 cut(s) 279
BstC8I GCNNGC 3 cut(s) 12, 280, 588
BstDEI CTNAG 7 cut(s) 36, 63, 136, 189, 231, 350, 455
BstENI CCTNNNNNAGG 1 cut(s) 458
BstF5I GGATG 2 cut(s) 574, 645
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 2 cut(s) 35, 60
BstKTI GATC 4 cut(s) 69, 489, 505, 544
BstMAI GTCTC 3 cut(s) 240, 436, 451
BstMBI GATC 4 cut(s) 66, 486, 502, 541
BstMWI GCNNNNNNNGC 4 cut(s) 30, 279, 362, 650
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 449
BstSCI CCNGG 2 cut(s) 162, 225
BstSLI GKGCMC 2 cut(s) 358, 592
BstV1I GCAGC 2 cut(s) 446, 617
BstX2I RGATCY 2 cut(s) 502, 541
BstYI RGATCY 2 cut(s) 502, 541
BsuI GTATCC 1 cut(s) 383
BsuRI GGCC 3 cut(s) 261, 278, 620
BtsCI GGATG 2 cut(s) 574, 645
BtsIMutI CAGTG 2 cut(s) 60, 666
Cac8I GCNNGC 3 cut(s) 12, 280, 588
CaiI CAGNNNCTG 1 cut(s) 279
CfoI GCGC 2 cut(s) 35, 60
Cfr10I RCCGGY 1 cut(s) 265
Cfr13I GGNCC 4 cut(s) 276, 521, 619, 637
CseI GACGC 1 cut(s) 440
Csp6I GTAC 1 cut(s) 424
CspAI ACCGGT 1 cut(s) 265
CviAII CATG 6 cut(s) 304, 373, 394, 446, 490, 526
CviQI GTAC 1 cut(s) 424
DdeI CTNAG 7 cut(s) 36, 63, 136, 189, 231, 350, 455
DpnI GATC 4 cut(s) 68, 488, 504, 543
DpnII GATC 4 cut(s) 66, 486, 502, 541
Eam1104I CTCTTC 2 cut(s) 143, 243
EarI CTCTTC 2 cut(s) 143, 243
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 1 cut(s) 507
Eco147I AGGCCT 1 cut(s) 261
Eco24I GRGCYC 2 cut(s) 12, 213
Eco47I GGWCC 2 cut(s) 521, 637
Eco53kI GAGCTC 1 cut(s) 10
EcoICRI GAGCTC 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 458
EcoO109I RGGNCCY 3 cut(s) 276, 521, 637
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 507
EcoT38I GRGCYC 2 cut(s) 12, 213
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 6 cut(s) 307, 376, 397, 449, 493, 529
FaiI YATR 9 cut(s) 71, 186, 285, 305, 374, 395, 447, 491, 527
FaqI GGGAC 1 cut(s) 623
FatI CATG 6 cut(s) 303, 372, 393, 445, 489, 525
FauI CCCGC 1 cut(s) 579
Fnu4HI GCNGC 2 cut(s) 435, 606
FokI GGATG 2 cut(s) 581, 632
FriOI GRGCYC 2 cut(s) 12, 213
Fsp4HI GCNGC 2 cut(s) 435, 606
FspAI RTGCGCAY 1 cut(s) 59
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 2 cut(s) 34, 59
GluI GCNGC 2 cut(s) 435, 606
GsaI CCCAGC 2 cut(s) 644, 653
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 3 cut(s) 261, 278, 620
HapII CCGG 4 cut(s) 30, 164, 266, 539
HgaI GACGC 1 cut(s) 440
HhaI GCGC 2 cut(s) 35, 60
Hin1II CATG 6 cut(s) 307, 376, 397, 449, 493, 529
Hin6I GCGC 2 cut(s) 33, 58
HinP1I GCGC 2 cut(s) 33, 58
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 4 cut(s) 82, 88, 233, 529
HpaII CCGG 4 cut(s) 30, 164, 266, 539
HphI GGTGA 1 cut(s) 343
Hpy188I TCNGA 3 cut(s) 87, 104, 257
Hpy188III TCNNGA 2 cut(s) 454, 484
HpyAV CCTTC 3 cut(s) 103, 108, 187
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 2 cut(s) 145, 317
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 279, 362, 650
HpyF3I CTNAG 7 cut(s) 36, 63, 136, 189, 231, 350, 455
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 6 cut(s) 307, 376, 397, 449, 493, 529
HspAI GCGC 2 cut(s) 33, 58
Kzo9I GATC 4 cut(s) 66, 486, 502, 541
LmnI GCTCC 3 cut(s) 7, 127, 178
Lsp1109I GCAGC 2 cut(s) 446, 617
LweI GCATC 1 cut(s) 45
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 2 cut(s) 46, 547
MalI GATC 4 cut(s) 68, 488, 504, 543
MboI GATC 4 cut(s) 66, 486, 502, 541
MboII GAAGA 3 cut(s) 160, 230, 393
MflI RGATCY 2 cut(s) 502, 541
MhlI GDGCHC 5 cut(s) 12, 102, 213, 358, 592
MluCI AATT 1 cut(s) 597
MlyI GAGTC 4 cut(s) 76, 82, 242, 523
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 2 cut(s) 477, 667
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 4 cut(s) 30, 164, 266, 539
MspR9I CCNGG 2 cut(s) 164, 227
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 4 cut(s) 30, 279, 362, 650
NciI CCSGG 1 cut(s) 164
NdeII GATC 4 cut(s) 66, 486, 502, 541
NlaIII CATG 6 cut(s) 307, 376, 397, 449, 493, 529
NlaIV GGNNCC 5 cut(s) 504, 523, 591, 621, 639
NmeAIII GCCGAG 1 cut(s) 40
NsbI TGCGCA 1 cut(s) 59
NspI RCATGY 1 cut(s) 449
PceI AGGCCT 1 cut(s) 261
PciI ACATGT 1 cut(s) 445
PdmI GAANNNNTTC 1 cut(s) 152
PfoI TCCNGGA 1 cut(s) 162
PinAI ACCGGT 1 cut(s) 265
PkrI GCNGC 2 cut(s) 436, 607
PleI GAGTC 4 cut(s) 76, 82, 241, 523
PpsI GAGTC 4 cut(s) 76, 82, 241, 523
PpuMI RGGWCCY 2 cut(s) 521, 637
PscI ACATGT 1 cut(s) 445
Psp124BI GAGCTC 1 cut(s) 12
Psp5II RGGWCCY 2 cut(s) 521, 637
Psp6I CCWGG 1 cut(s) 225
PspFI CCCAGC 2 cut(s) 640, 649
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 5 cut(s) 504, 523, 591, 621, 639
PspPI GGNCC 4 cut(s) 276, 521, 619, 637
PspPPI RGGWCCY 2 cut(s) 521, 637
PstNI CAGNNNCTG 1 cut(s) 279
PsuI RGATCY 2 cut(s) 502, 541
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
SacI GAGCTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 477, 667
SatI GCNGC 2 cut(s) 435, 606
Sau3AI GATC 4 cut(s) 66, 486, 502, 541
Sau96I GGNCC 4 cut(s) 276, 521, 619, 637
SchI GAGTC 4 cut(s) 76, 82, 242, 523
ScrFI CCNGG 2 cut(s) 164, 227
SduI GDGCHC 5 cut(s) 12, 102, 213, 358, 592
SfaNI GCATC 1 cut(s) 45
SinI GGWCC 2 cut(s) 521, 637
Sse9I AATT 1 cut(s) 597
SseBI AGGCCT 1 cut(s) 261
SsiI CCGC 1 cut(s) 586
SstI GAGCTC 1 cut(s) 12
StuI AGGCCT 1 cut(s) 261
StyD4I CCNGG 2 cut(s) 162, 225
StyI CCWWGG 1 cut(s) 507
TaiI ACGT 1 cut(s) 99
TaqI TCGA 1 cut(s) 495
TasI AATT 1 cut(s) 597
TatI WGTACW 1 cut(s) 423
Tru1I TTAA 2 cut(s) 477, 667
Tru9I TTAA 2 cut(s) 477, 667
TscAI CASTG 2 cut(s) 67, 666
TseI GCWGC 2 cut(s) 434, 605
TspDTI ATGAA 1 cut(s) 389
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 2 cut(s) 67, 666
VpaK11BI GGWCC 2 cut(s) 521, 637
XagI CCTNNNNNAGG 1 cut(s) 458
XceI RCATGY 1 cut(s) 449
XmnI GAANNNNTTC 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.