FvH4_2g31150

Belongs to the universal ribosomal protein uS3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001560
Physical Location & Seq
Forward (+)
23959993 .. 23962019
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31150.t1

Sequence Viewer

Length: 747 bp
ATGGCGGCCACTCAGGCTCAGATGAGCAAAAAACGAAAGTTTGTTGCCGACGGAGTTTTCTTTGCCGAATTGAATGAGGTTCTGACCAGAGAGCTCGCGGAGGATGGTTACTCCGGTGTCGAAGTCAGGGTCACCCCTGTACGCACTGAGATCATCATCAGAGCCACTCGCACCCAAAACGTTCTGGGTGAGAAGGGGAGGAGGATCAGGGAGCTGACCTCATTAGTTCAGAAGAGGTTCAAGTTTCCCGAGGGAAGCGTAGAGCTTTATGCCGAGAAGGTTAACAATAGAGGACTCTGTGCCATTGCGCAGGCCGAGTCTCTCCGGTACAAACTCCTTGGAGGCCTTGCCGTTCGCAGGGCATGTTATGGTGTGTTGAGATTTGTCATGGAGAGTGGCGCAAAAGGATGTGAGGTTATTGTAAGTGGAAAGCTCAGGGCACAACGTGCCAAGGCGATGAAGTTTAAGGATGGCTATATGATATCCTCTGGTCAACCTGTTAATGAGTACATTGACTCTGCTGTTAGGCATGTTCTTCTTAGACAGGGTGTGCTCGGTATTAAGGTGAAGATCATGCTCGACTGGGATCCCAAGGGGAAGCAAGGACCAATGACCCCTCTCCCTGATTTGGTGACCATCCATACTCCCAAAGAAGAGCTCTATACTCCAGCTCCTCCAGCGGCGGCAATCAACTTGGATGCGCTTCCATCTCCTGTTGCCATGCCCGCCCCTGTTTCTGTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
KH_2 Ribosomal_S3_C

Protein Analysis

249

Amino Acids

27.25

Weight (kDa)

9.7

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_2 PF07650 23 - 97 6.1e-15 KH domain
Ribosomal_S3_C PF00189 109 - 191 2.9e-26 Ribosomal protein S3, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 309
AccII CGCG 1 cut(s) 98
AciI CCGC 5 cut(s) 5, 98, 680, 683, 726
AclI AACGTT 1 cut(s) 180
AclWI GGATC 3 cut(s) 212, 581, 594
AcoI YGGCCR 1 cut(s) 6
AdeI CACNNNGTG 1 cut(s) 446
AfaI GTAC 3 cut(s) 141, 329, 509
AfiI CCNNNNNNNGG 2 cut(s) 357, 628
AgsI TTSAA 2 cut(s) 73, 241
AluBI AGCT 6 cut(s) 94, 214, 265, 433, 658, 671
AluI AGCT 6 cut(s) 94, 214, 265, 433, 658, 671
Alw21I GWGCWC 3 cut(s) 96, 555, 660
Alw26I GTCTC 1 cut(s) 324
AlwI GGATC 3 cut(s) 212, 581, 594
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 3 cut(s) 6, 312, 343
ArsI GACNNNNNNTTYG 2 cut(s) 114, 146
Asp700I GAANNNNTTC 1 cut(s) 236
AspLEI GCGC 3 cut(s) 310, 401, 703
AspS9I GGNCC 1 cut(s) 605
AsuHPI GGTGA 4 cut(s) 124, 200, 577, 643
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 605
BaeGI GKGCMC 1 cut(s) 442
BamHI GGATCC 1 cut(s) 586
BanII GRGCYC 2 cut(s) 96, 660
Bbv12I GWGCWC 3 cut(s) 96, 555, 660
BccI CCATC 4 cut(s) 98, 464, 644, 715
BceAI ACGGC 1 cut(s) 335
BcoDI GTCTC 1 cut(s) 324
BfmI CTRYAG 1 cut(s) 738
BglI GCCNNNNNGGC 1 cut(s) 14
BisI GCNGC 3 cut(s) 6, 681, 684
BlsI GCNGC 3 cut(s) 7, 682, 685
Bme18I GGWCC 1 cut(s) 605
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 1 cut(s) 588
BmrI ACTGGG 1 cut(s) 592
BmsI GCATC 1 cut(s) 688
BmuI ACTGGG 1 cut(s) 592
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpmI CTGGAG 2 cut(s) 651, 660
Bpu10I CCTNAGC 1 cut(s) 434
BsaBI GATNNNNATC 1 cut(s) 155
BsaJI CCNNGG 4 cut(s) 249, 337, 450, 591
BsaWI WCCGGW 2 cut(s) 113, 324
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bsc4I CCNNNNNNNGG 2 cut(s) 357, 628
Bse1I ACTGG 1 cut(s) 587
Bse3DI GCAATG 1 cut(s) 303
Bse8I GATNNNNATC 1 cut(s) 155
BseDI CCNNGG 4 cut(s) 249, 337, 450, 591
BseGI GGATG 5 cut(s) 109, 413, 475, 636, 703
BseJI GATNNNNATC 1 cut(s) 155
BseLI CCNNNNNNNGG 2 cut(s) 357, 628
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 4 cut(s) 26, 32, 138, 448
BseNI ACTGG 1 cut(s) 587
BseRI GAGGAG 2 cut(s) 214, 663
BseSI GKGCMC 1 cut(s) 442
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 3 cut(s) 8, 314, 345
BsiHKAI GWGCWC 3 cut(s) 96, 555, 660
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 2 cut(s) 114, 325
BslI CCNNNNNNNGG 2 cut(s) 357, 628
BsmAI GTCTC 1 cut(s) 324
BsnI GGCC 3 cut(s) 8, 314, 345
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 4 cut(s) 96, 442, 555, 660
Bsp143I GATC 4 cut(s) 150, 204, 570, 586
BspACI CCGC 5 cut(s) 5, 98, 680, 683, 726
BspANI GGCC 3 cut(s) 8, 314, 345
BspCNI CTCAG 4 cut(s) 25, 31, 139, 447
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 1 cut(s) 588
BspPI GGATC 3 cut(s) 212, 581, 594
BspQI GCTCTTC 1 cut(s) 648
BsrDI GCAATG 1 cut(s) 303
BsrI ACTGG 1 cut(s) 587
BssECI CCNNGG 4 cut(s) 249, 337, 450, 591
BssMI GATC 4 cut(s) 150, 204, 570, 586
BssT1I CCWWGG 3 cut(s) 337, 450, 591
Bst6I CTCTTC 2 cut(s) 227, 648
BstAPI GCANNNNNTGC 1 cut(s) 446
BstC8I GCNNGC 3 cut(s) 96, 312, 726
BstDEI CTNAG 5 cut(s) 12, 18, 147, 434, 539
BstEII GGTNACC 2 cut(s) 130, 631
BstF5I GGATG 5 cut(s) 109, 413, 475, 636, 703
BstFNI CGCG 1 cut(s) 98
BstHHI GCGC 3 cut(s) 310, 401, 703
BstKTI GATC 4 cut(s) 153, 207, 573, 589
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 4 cut(s) 150, 204, 570, 586
BstMWI GCNNNNNNNGC 4 cut(s) 14, 446, 677, 725
BstNSI RCATGY 2 cut(s) 366, 533
BstPI GGTNACC 2 cut(s) 130, 631
BstSFI CTRYAG 1 cut(s) 738
BstSLI GKGCMC 1 cut(s) 442
BstUI CGCG 1 cut(s) 98
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BsuRI GGCC 3 cut(s) 8, 314, 345
BtgZI GCGATG 1 cut(s) 470
BtsCI GGATG 5 cut(s) 109, 413, 475, 636, 703
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 3 cut(s) 96, 312, 726
CfoI GCGC 3 cut(s) 310, 401, 703
Cfr13I GGNCC 1 cut(s) 605
Csp6I GTAC 3 cut(s) 140, 328, 508
CviAII CATG 5 cut(s) 363, 388, 530, 574, 721
CviQI GTAC 3 cut(s) 140, 328, 508
DdeI CTNAG 5 cut(s) 12, 18, 147, 434, 539
DpnI GATC 4 cut(s) 152, 206, 572, 588
DpnII GATC 4 cut(s) 150, 204, 570, 586
DraIII CACNNNGTG 1 cut(s) 446
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 2 cut(s) 227, 648
EarI CTCTTC 2 cut(s) 227, 648
Ecl136II GAGCTC 2 cut(s) 94, 658
Eco130I CCWWGG 3 cut(s) 337, 450, 591
Eco147I AGGCCT 1 cut(s) 345
Eco24I GRGCYC 2 cut(s) 96, 660
Eco32I GATATC 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 605
Eco53kI GAGCTC 2 cut(s) 94, 658
Eco88I CYCGRG 1 cut(s) 248
Eco91I GGTNACC 2 cut(s) 130, 631
EcoICRI GAGCTC 2 cut(s) 94, 658
EcoO65I GGTNACC 2 cut(s) 130, 631
EcoRV GATATC 1 cut(s) 483
EcoT14I CCWWGG 3 cut(s) 337, 450, 591
EcoT38I GRGCYC 2 cut(s) 96, 660
ErhI CCWWGG 3 cut(s) 337, 450, 591
FaeI CATG 5 cut(s) 366, 391, 533, 577, 724
FatI CATG 5 cut(s) 362, 387, 529, 573, 720
FauI CCCGC 1 cut(s) 733
Fnu4HI GCNGC 3 cut(s) 6, 681, 684
FokI GGATG 5 cut(s) 116, 420, 482, 623, 710
FriOI GRGCYC 2 cut(s) 96, 660
Fsp4HI GCNGC 3 cut(s) 6, 681, 684
FspI TGCGCA 1 cut(s) 309
GlaI GCGC 3 cut(s) 309, 400, 702
GluI GCNGC 3 cut(s) 6, 681, 684
GsuI CTGGAG 2 cut(s) 651, 660
HaeIII GGCC 3 cut(s) 8, 314, 345
HapII CCGG 2 cut(s) 114, 325
HhaI GCGC 3 cut(s) 310, 401, 703
Hin1II CATG 5 cut(s) 366, 391, 533, 577, 724
Hin6I GCGC 3 cut(s) 308, 399, 701
HinP1I GCGC 3 cut(s) 308, 399, 701
HincII GTYRAC 2 cut(s) 283, 494
HindII GTYRAC 2 cut(s) 283, 494
HinfI GANTC 3 cut(s) 294, 317, 515
HpaI GTTAAC 1 cut(s) 283
HpaII CCGG 2 cut(s) 114, 325
HphI GGTGA 4 cut(s) 124, 200, 577, 643
Hpy166II GTNNAC 2 cut(s) 283, 494
Hpy188I TCNGA 4 cut(s) 21, 84, 161, 231
Hpy188III TCNNGA 1 cut(s) 248
Hpy8I GTNNAC 2 cut(s) 283, 494
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 187, 271
HpyCH4IV ACGT 2 cut(s) 180, 445
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 446, 677, 725
HpyF3I CTNAG 5 cut(s) 12, 18, 147, 434, 539
HpySE526I ACGT 2 cut(s) 180, 445
Hsp92II CATG 5 cut(s) 366, 391, 533, 577, 724
HspAI GCGC 3 cut(s) 308, 399, 701
KspAI GTTAAC 1 cut(s) 283
Kzo9I GATC 4 cut(s) 150, 204, 570, 586
LguI GCTCTTC 1 cut(s) 648
LmnI GCTCC 2 cut(s) 211, 676
LweI GCATC 1 cut(s) 688
MaeII ACGT 2 cut(s) 180, 445
MaeIII GTNAC 3 cut(s) 107, 130, 631
MalI GATC 4 cut(s) 152, 206, 572, 588
MboI GATC 4 cut(s) 150, 204, 570, 586
MboII GAAGA 4 cut(s) 244, 527, 580, 665
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 4 cut(s) 96, 442, 555, 660
MluCI AATT 1 cut(s) 68
MlyI GAGTC 3 cut(s) 288, 326, 509
MroXI GAANNNNTTC 1 cut(s) 236
MseI TTAA 5 cut(s) 282, 465, 501, 561, 745
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 2 cut(s) 114, 325
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 4 cut(s) 14, 446, 677, 725
NdeII GATC 4 cut(s) 150, 204, 570, 586
NlaIII CATG 5 cut(s) 366, 391, 533, 577, 724
NlaIV GGNNCC 1 cut(s) 588
NmeAIII GCCGAG 2 cut(s) 298, 340
NmuCI GTSAC 2 cut(s) 130, 631
NsbI TGCGCA 1 cut(s) 309
NspI RCATGY 2 cut(s) 366, 533
PceI AGGCCT 1 cut(s) 345
PciSI GCTCTTC 1 cut(s) 648
PdmI GAANNNNTTC 1 cut(s) 236
PkrI GCNGC 3 cut(s) 7, 682, 685
PleI GAGTC 3 cut(s) 288, 325, 509
PpsI GAGTC 3 cut(s) 288, 325, 509
Psp124BI GAGCTC 2 cut(s) 96, 660
Psp1406I AACGTT 1 cut(s) 180
PspEI GGTNACC 2 cut(s) 130, 631
PspN4I GGNNCC 1 cut(s) 588
PspPI GGNCC 1 cut(s) 605
PsuI RGATCY 1 cut(s) 586
RsaI GTAC 3 cut(s) 141, 329, 509
RsaNI GTAC 3 cut(s) 140, 328, 508
SacI GAGCTC 2 cut(s) 96, 660
SapI GCTCTTC 1 cut(s) 648
SaqAI TTAA 5 cut(s) 282, 465, 501, 561, 745
SatI GCNGC 3 cut(s) 6, 681, 684
Sau3AI GATC 4 cut(s) 150, 204, 570, 586
Sau96I GGNCC 1 cut(s) 605
SchI GAGTC 3 cut(s) 288, 326, 509
SduI GDGCHC 4 cut(s) 96, 442, 555, 660
SfaNI GCATC 1 cut(s) 688
SfcI CTRYAG 1 cut(s) 738
SinI GGWCC 1 cut(s) 605
Sse9I AATT 1 cut(s) 68
SseBI AGGCCT 1 cut(s) 345
SsiI CCGC 5 cut(s) 5, 98, 680, 683, 726
SstI GAGCTC 2 cut(s) 96, 660
StuI AGGCCT 1 cut(s) 345
StyI CCWWGG 3 cut(s) 337, 450, 591
TaiI ACGT 2 cut(s) 183, 448
TaqI TCGA 2 cut(s) 120, 579
TasI AATT 1 cut(s) 68
TatI WGTACW 1 cut(s) 507
TauI GCSGC 3 cut(s) 8, 683, 686
Tru1I TTAA 5 cut(s) 282, 465, 501, 561, 745
Tru9I TTAA 5 cut(s) 282, 465, 501, 561, 745
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 2 cut(s) 130, 631
Tsp45I GTSAC 2 cut(s) 130, 631
TspDTI ATGAA 1 cut(s) 473
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 605
XceI RCATGY 2 cut(s) 366, 533
XmnI GAANNNNTTC 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.