pycom15g04960

Belongs to the universal ribosomal protein uS3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001560
Physical Location & Seq
Forward (+)
2979093 .. 2980671
1579 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g04960.3

Sequence Viewer

Length: 717 bp
ATGGCGGCAGCTACCCAGATGAGCAAGAAGCGAAAGTTTGTGGCCGACGGAGTTTTCTTCGCCGAGCTGAACGAGGTTCTGACCAGAGAGCTGGCGGAGGACGGCTACTCCGGCGTCGAAGTTAGGGTCACCCCTGTCCGGACTGAGATCATCATCCGAGCCACTCGCACTCAGAACGTCCTTGGCGAGAAGGGAAGAAGGATTAGGGAGCTGACATCTATTGTTCAGAAGCGGTTCAAGTTCCCGGAGAACAGCGTCGAGCTCTATGCTGAGAAGGTGAACAACAGAGGACTCTGTGCCATTGCTCAGGCTGAGTCTCTTCGTTACAAGCTCCTCGGAGGTCTTGCTGTTCGCAGGGCTTGTTATGGTGTGTTGAGATTTGTCATGGAGAGTGGTGCAAAGGGATGTGAGGTTATTGTGAGTGGAAAGCTCAGGGCAGCGCGTGCCAAGTCCATGAAGTTTAAGGATGGCTACATGATTTCCTCTGGTCAGCCCGTCAATGAGTACATTGATGCCGCTGTGAGGCACGTTCTTCTTAGACAGGGTGTGCTTGGTATTAAGGTGAAGATCATGCTCGACTGGGATCCTAAGGGCAAGCAGGGACCAATGACTCCATTGCCTGATTTGGTGACCATCCACACCCCCAAGGAAGAACAATACCTTCCAGTTCCACCCGCAGCAATCGACATAGAGCCAGTTCCAGTTGCAGTAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
KH_2 Ribosomal_S3_C

Protein Analysis

239

Amino Acids

26.34

Weight (kDa)

9.63

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 442
AccIII TCCGGA 1 cut(s) 138
AciI CCGC 5 cut(s) 5, 95, 232, 516, 675
AclWI GGATC 2 cut(s) 578, 591
AcoI YGGCCR 1 cut(s) 42
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 2 cut(s) 138, 522
AgsI TTSAA 1 cut(s) 238
AluBI AGCT 8 cut(s) 11, 67, 91, 211, 262, 331, 430, 713
AluI AGCT 8 cut(s) 11, 67, 91, 211, 262, 331, 430, 713
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 2 cut(s) 578, 591
Aor13HI TCCGGA 1 cut(s) 138
AoxI GGCC 1 cut(s) 42
ApeKI GCWGC 3 cut(s) 8, 437, 677
ArsI GACNNNNNNTTYG 2 cut(s) 111, 143
Asp700I GAANNNNTTC 1 cut(s) 233
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 1 cut(s) 602
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 4 cut(s) 121, 289, 574, 640
AvaII GGWCC 1 cut(s) 602
AxyI CCTNAGG 1 cut(s) 588
BamHI GGATCC 1 cut(s) 583
BanII GRGCYC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 3 cut(s) 20, 449, 689
BccI CCATC 2 cut(s) 461, 641
BceAI ACGGC 1 cut(s) 118
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 1 cut(s) 321
BisI GCNGC 5 cut(s) 6, 9, 438, 516, 678
BlsI GCNGC 5 cut(s) 7, 10, 439, 517, 679
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 1 cut(s) 602
BmiI GGNNCC 2 cut(s) 585, 603
BmrFI CCNGG 1 cut(s) 245
BmrI ACTGGG 1 cut(s) 589
BmsI GCATC 1 cut(s) 502
BmuI ACTGGG 1 cut(s) 589
Bpu10I CCTNAGC 2 cut(s) 306, 431
BpuMI CCSGG 1 cut(s) 245
BsaBI GATNNNNATC 1 cut(s) 152
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 3 cut(s) 181, 334, 645
BsaWI WCCGGW 1 cut(s) 138
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 522
Bse1I ACTGG 4 cut(s) 584, 665, 695, 701
Bse21I CCTNAGG 1 cut(s) 588
Bse3DI GCAATG 2 cut(s) 300, 614
Bse8I GATNNNNATC 1 cut(s) 152
BseAI TCCGGA 1 cut(s) 138
BseDI CCNNGG 3 cut(s) 181, 334, 645
BseGI GGATG 4 cut(s) 153, 410, 472, 633
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 2 cut(s) 138, 522
BseMI GCAATG 2 cut(s) 300, 614
BseMII CTCAG 6 cut(s) 135, 185, 261, 303, 320, 445
BseNI ACTGG 4 cut(s) 584, 665, 695, 701
BseRI GAGGAG 1 cut(s) 323
BseXI GCAGC 3 cut(s) 20, 449, 689
Bsh1236I CGCG 1 cut(s) 442
BshFI GGCC 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 3 cut(s) 111, 139, 245
BslFI GGGAC 1 cut(s) 615
BslI CCNNNNNNNGG 2 cut(s) 138, 522
BsmAI GTCTC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 615
BsnI GGCC 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 264
Bsp13I TCCGGA 1 cut(s) 138
Bsp143I GATC 3 cut(s) 147, 567, 583
BspACI CCGC 5 cut(s) 5, 95, 232, 516, 675
BspANI GGCC 1 cut(s) 44
BspCNI CTCAG 6 cut(s) 136, 184, 262, 304, 319, 444
BspEI TCCGGA 1 cut(s) 138
BspFNI CGCG 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 585, 603
BspPI GGATC 2 cut(s) 578, 591
BsrDI GCAATG 2 cut(s) 300, 614
BsrI ACTGG 4 cut(s) 584, 665, 695, 701
BssECI CCNNGG 3 cut(s) 181, 334, 645
BssMI GATC 3 cut(s) 147, 567, 583
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 181, 645
Bst6I CTCTTC 1 cut(s) 324
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 443
BstC8I GCNNGC 3 cut(s) 93, 444, 596
BstDEI CTNAG 8 cut(s) 144, 171, 270, 306, 312, 431, 536, 588
BstEII GGTNACC 2 cut(s) 127, 628
BstF5I GGATG 4 cut(s) 153, 410, 472, 633
BstFNI CGCG 1 cut(s) 442
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 3 cut(s) 150, 570, 586
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 3 cut(s) 147, 567, 583
BstMWI GCNNNNNNNGC 2 cut(s) 111, 443
BstPI GGTNACC 2 cut(s) 127, 628
BstSCI CCNGG 1 cut(s) 243
BstUI CGCG 1 cut(s) 442
BstV1I GCAGC 3 cut(s) 20, 449, 689
BstX2I RGATCY 1 cut(s) 583
BstXI CCANNNNNNTGG 1 cut(s) 91
BstYI RGATCY 1 cut(s) 583
Bsu36I CCTNAGG 1 cut(s) 588
BsuRI GGCC 1 cut(s) 44
BtsCI GGATG 4 cut(s) 153, 410, 472, 633
Cac8I GCNNGC 3 cut(s) 93, 444, 596
CfoI GCGC 1 cut(s) 442
Cfr13I GGNCC 1 cut(s) 602
CseI GACGC 2 cut(s) 103, 244
Csp6I GTAC 1 cut(s) 505
CviAII CATG 4 cut(s) 385, 454, 475, 571
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 8 cut(s) 144, 171, 270, 306, 312, 431, 536, 588
DpnI GATC 3 cut(s) 149, 569, 585
DpnII GATC 3 cut(s) 147, 567, 583
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
EciI GGCGGA 1 cut(s) 110
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 2 cut(s) 181, 645
Eco24I GRGCYC 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 602
Eco53kI GAGCTC 1 cut(s) 262
Eco81I CCTNAGG 1 cut(s) 588
Eco91I GGTNACC 2 cut(s) 127, 628
EcoICRI GAGCTC 1 cut(s) 262
EcoO65I GGTNACC 2 cut(s) 127, 628
EcoT14I CCWWGG 2 cut(s) 181, 645
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 2 cut(s) 181, 645
FaeI CATG 4 cut(s) 388, 457, 478, 574
FaiI YATR 7 cut(s) 267, 366, 386, 455, 476, 572, 689
FaqI GGGAC 1 cut(s) 615
FatI CATG 4 cut(s) 384, 453, 474, 570
FauI CCCGC 1 cut(s) 682
Fnu4HI GCNGC 5 cut(s) 6, 9, 438, 516, 678
FokI GGATG 4 cut(s) 140, 417, 479, 620
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 5 cut(s) 6, 9, 438, 516, 678
GlaI GCGC 1 cut(s) 441
GluI GCNGC 5 cut(s) 6, 9, 438, 516, 678
HaeIII GGCC 1 cut(s) 44
HapII CCGG 3 cut(s) 111, 139, 245
HgaI GACGC 2 cut(s) 103, 244
HhaI GCGC 1 cut(s) 442
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 4 cut(s) 388, 457, 478, 574
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HinfI GANTC 3 cut(s) 291, 314, 610
HpaII CCGG 3 cut(s) 111, 139, 245
HphI GGTGA 4 cut(s) 121, 289, 574, 640
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 5 cut(s) 81, 158, 174, 228, 338
Hpy188III TCNNGA 1 cut(s) 139
Hpy8I GTNNAC 1 cut(s) 280
Hpy99I CGWCG 3 cut(s) 50, 119, 260
HpyAV CCTTC 4 cut(s) 184, 192, 268, 671
HpyCH4IV ACGT 2 cut(s) 177, 528
HpyCH4V TGCA 2 cut(s) 398, 707
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 443
HpyF3I CTNAG 8 cut(s) 144, 171, 270, 306, 312, 431, 536, 588
HpySE526I ACGT 2 cut(s) 177, 528
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 4 cut(s) 388, 457, 478, 574
HspAI GCGC 1 cut(s) 440
Kpn2I TCCGGA 1 cut(s) 138
Kzo9I GATC 3 cut(s) 147, 567, 583
LmnI GCTCC 2 cut(s) 208, 336
Lsp1109I GCAGC 3 cut(s) 20, 449, 689
LweI GCATC 1 cut(s) 502
MaeII ACGT 2 cut(s) 177, 528
MaeIII GTNAC 3 cut(s) 127, 323, 628
MalI GATC 3 cut(s) 149, 569, 585
MboI GATC 3 cut(s) 147, 567, 583
MboII GAAGA 6 cut(s) 49, 207, 311, 524, 577, 662
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 1 cut(s) 264
MlyI GAGTC 3 cut(s) 285, 323, 604
MnlI CCTC 8 cut(s) 67, 91, 281, 332, 344, 403, 493, 516
MroI TCCGGA 1 cut(s) 138
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 3 cut(s) 462, 558, 715
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 3 cut(s) 111, 139, 245
MspR9I CCNGG 1 cut(s) 245
MvnI CGCG 1 cut(s) 442
MwoI GCNNNNNNNGC 2 cut(s) 111, 443
NciI CCSGG 1 cut(s) 245
NdeII GATC 3 cut(s) 147, 567, 583
NlaIII CATG 4 cut(s) 388, 457, 478, 574
NlaIV GGNNCC 2 cut(s) 585, 603
NmeAIII GCCGAG 1 cut(s) 88
NmuCI GTSAC 2 cut(s) 127, 628
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 233
PfoI TCCNGGA 1 cut(s) 243
PkrI GCNGC 5 cut(s) 7, 10, 439, 517, 679
PleI GAGTC 3 cut(s) 285, 322, 604
PpsI GAGTC 3 cut(s) 285, 322, 604
Psp124BI GAGCTC 1 cut(s) 264
PspEI GGTNACC 2 cut(s) 127, 628
PspN4I GGNNCC 2 cut(s) 585, 603
PspPI GGNCC 1 cut(s) 602
PsuI RGATCY 1 cut(s) 583
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SacI GAGCTC 1 cut(s) 264
SaqAI TTAA 3 cut(s) 462, 558, 715
SatI GCNGC 5 cut(s) 6, 9, 438, 516, 678
Sau3AI GATC 3 cut(s) 147, 567, 583
Sau96I GGNCC 1 cut(s) 602
SchI GAGTC 3 cut(s) 285, 323, 604
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 1 cut(s) 264
SfaNI GCATC 1 cut(s) 502
SinI GGWCC 1 cut(s) 602
SsiI CCGC 5 cut(s) 5, 95, 232, 516, 675
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 243
StyI CCWWGG 2 cut(s) 181, 645
TaiI ACGT 2 cut(s) 180, 531
TaqI TCGA 4 cut(s) 117, 258, 576, 684
TatI WGTACW 1 cut(s) 504
TauI GCSGC 2 cut(s) 8, 518
Tru1I TTAA 3 cut(s) 462, 558, 715
Tru9I TTAA 3 cut(s) 462, 558, 715
TseFI GTSAC 2 cut(s) 127, 628
TseI GCWGC 3 cut(s) 8, 437, 677
Tsp45I GTSAC 2 cut(s) 127, 628
TspDTI ATGAA 1 cut(s) 470
TspGWI ACGGA 1 cut(s) 63
VpaK11BI GGWCC 1 cut(s) 602
XmnI GAANNNNTTC 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.