FvH4_1g29930

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001443
Physical Location & Seq
Forward (+)
23674031 .. 23675741
1711 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29930.t1

Sequence Viewer

Length: 1347 bp
ATGATTTTATCCTGGAGAAGAAGAAGAGCCAGCAAGTATGCCAACAGGAAGGGATTTTCCGGCGAGAATCTAGGCATGGGGATGGAGGAGATAGTGATTCCTTCCCACTTCCGGTGCCCTATCACTCTCGACCTCATGAAAGATCCGGTCACTTTGTCGACGGGGATGACCTACGACAGGTCCAGCATTGACGCCTGGATCGAGAAAGGTCACAACACCTGCCCGGTCACCAATCAAGTTCTGGCGACGTTCGACCAAATCCCCAACCACGCCTTACGTAAGATGATCCAAGGCTGGTGCGTCCAGAACCGCTCTCACGGCGTCGAACGCATCCCCACGCCTCGGATTCCGGTCTCTGCGTACGAGGTCACTAGGGTTTATCAGAGAATGATGGCTGTGACTCAACGCGGCGATTACAACAAGTGTTTGGAATTGGTGGCCAAGATCAGAGCTTGGGGAAAAGACAGCGAGCGCAACAAGAAGTGTATGGTGGAGAACAGAATGGGTTGCTGCTTGTCTGCCACTTTCGAGTTCTTCGCGGAAAATGAGGCAGCGACGTTGTTGAAGGAGGAGATATTGTCCGTGATGACATGGATGTTTCCGATGGGTGAAGAAGGCCAATCAAGGCTTGGATCTGCCAAGTCGTTGAGATGTTTGGTGTGGTTTTTGGAGGGTAAAGATCTCTCTTCAAGGCAAAACGCAGTTTTGGTATTGAGAAAGTTGCTTTCTTTGGATCAAAAACATGTAGATGGGATTGTGCAGATTGAAGGAGCCATTGAAGCATTGGTGAAGATAATTAGGGAGGGCCCTAGTATCTGTCCTACTGCTACTAAAGCTTCCCTATCATGTGTCTTCTACATGATTTCAGGAGCAAGTGATGAAATCAAATCAAGATTTGTGGAATTGGGTTTGGTTTCTCTGTTGCTAGAAATACTTGTTGATGCAGAGAAGAGCCTATGTGAGAGGGCTTTGGGGGTTTTGGAGGGTCTTTGTGATTGCAAGCAAGGGAGAGCAAAGGCCTATGAGAATTCTCTAACCATGCCTCTTTTGGTTAAGAAGATGTTAAGGGTATCGGCCTTGGCAAATGAGTTTTCGGTTTCTATAATTTGGAAGCTCTGCAAAAACCAAAACGGTGATGGTGATGATGAGGAAGAAATAAGGATTCTGATGGAGGCACTTCAAGTGGGCGCATTTCAGAAGCTCTTGGTTCTGTTGCAGGTTGGATGTGGTGATGAAATCAAGGACAAAGTTACTGAGTTGTTGAAGTTGTTCAATCTTCACATCAGTAAATTAGACTGTGTTGATTCATCCATGGATTTTAAGTATTTGAAGAAAGGCCAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

449

Amino Acids

50.43

Weight (kDa)

8.38

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 33 - 104 1.3e-21 U-box domain
ARM_PUB PF25598 122 - 442 1.1e-73 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 227
Acc36I ACCTGC 2 cut(s) 227, 1207
AccB1I GGYRCC 1 cut(s) 114
AccBSI CCGCTC 1 cut(s) 312
AccI GTMKAC 1 cut(s) 158
AccII CGCG 2 cut(s) 408, 539
AciI CCGC 3 cut(s) 310, 408, 539
AclWI GGATC 5 cut(s) 137, 206, 280, 640, 741
AcoI YGGCCR 1 cut(s) 438
AcsI RAATTY 1 cut(s) 1027
AcyI GRCGYC 2 cut(s) 192, 321
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 3 cut(s) 111, 177, 342
AflIII ACRYGT 1 cut(s) 742
AgsI TTSAA 8 cut(s) 565, 690, 767, 779, 1181, 1264, 1273, 1330
AjnI CCWGG 2 cut(s) 11, 194
AluBI AGCT 4 cut(s) 452, 836, 1114, 1201
AluI AGCT 4 cut(s) 452, 836, 1114, 1201
Alw26I GTCTC 1 cut(s) 358
AlwI GGATC 5 cut(s) 137, 206, 280, 640, 741
AoxI GGCC 6 cut(s) 438, 616, 805, 1017, 1074, 1336
ApaI GGGCCC 1 cut(s) 809
ApeKI GCWGC 2 cut(s) 510, 551
ApoI RAATTY 1 cut(s) 1027
Asp700I GAANNNNTTC 1 cut(s) 1268
AspLEI GCGC 2 cut(s) 474, 1190
AspS9I GGNCC 3 cut(s) 180, 805, 806
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 6 cut(s) 220, 620, 799, 1145, 1151, 1241
AvaII GGWCC 1 cut(s) 180
BaeGI GKGCMC 2 cut(s) 119, 809
BalI TGGCCA 1 cut(s) 440
BanI GGYRCC 1 cut(s) 114
BanII GRGCYC 1 cut(s) 809
BbsI GAAGAC 1 cut(s) 844
BbvI GCAGC 2 cut(s) 497, 563
BccI CCATC 6 cut(s) 76, 385, 598, 743, 1130, 1162
BceAI ACGGC 1 cut(s) 334
BciT130I CCWGG 2 cut(s) 13, 196
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 358
BfaI CTAG 4 cut(s) 71, 372, 810, 926
BfuAI ACCTGC 2 cut(s) 227, 1207
BglII AGATCT 1 cut(s) 679
BisI GCNGC 3 cut(s) 409, 511, 552
BlsI GCNGC 3 cut(s) 410, 512, 553
Bme1390I CCNGG 3 cut(s) 13, 196, 224
Bme18I GGWCC 1 cut(s) 180
BmgT120I GGNCC 3 cut(s) 180, 805, 806
BmiI GGNNCC 3 cut(s) 116, 772, 807
BmrFI CCNGG 3 cut(s) 13, 196, 224
BmsI GCATC 2 cut(s) 339, 931
BpiI GAAGAC 1 cut(s) 844
BpmI CTGGAG 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 224
BsaAI YACGTR 1 cut(s) 278
BsaHI GRCGYC 2 cut(s) 192, 321
BsaI GGTCTC 1 cut(s) 358
BsaJI CCNNGG 4 cut(s) 289, 341, 1077, 1311
BsaWI WCCGGW 3 cut(s) 111, 145, 349
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 177, 342
BseBI CCWGG 2 cut(s) 13, 196
BseDI CCNNGG 4 cut(s) 289, 341, 1077, 1311
BseGI GGATG 6 cut(s) 87, 171, 330, 600, 1229, 1307
BseLI CCNNNNNNNGG 3 cut(s) 111, 177, 342
BseMII CTCAG 1 cut(s) 1245
BseRI GAGGAG 2 cut(s) 101, 584
BseSI GKGCMC 2 cut(s) 119, 809
BseXI GCAGC 2 cut(s) 497, 563
BsgI GTGCAG 1 cut(s) 779
Bsh1236I CGCG 2 cut(s) 408, 539
BshFI GGCC 6 cut(s) 440, 618, 807, 1019, 1076, 1338
BshNI GGYRCC 1 cut(s) 114
BsiSI CCGG 5 cut(s) 60, 112, 146, 224, 350
BsiWI CGTACG 1 cut(s) 360
BslI CCNNNNNNNGG 3 cut(s) 111, 177, 342
BsmAI GTCTC 1 cut(s) 358
BsnI GGCC 6 cut(s) 440, 618, 807, 1019, 1076, 1338
Bso31I GGTCTC 1 cut(s) 358
Bsp120I GGGCCC 1 cut(s) 805
Bsp1286I GDGCHC 2 cut(s) 119, 809
Bsp143I GATC 7 cut(s) 142, 198, 285, 444, 632, 679, 733
Bsp19I CCATGG 1 cut(s) 1311
BspACI CCGC 3 cut(s) 310, 408, 539
BspANI GGCC 6 cut(s) 440, 618, 807, 1019, 1076, 1338
BspCNI CTCAG 1 cut(s) 1246
BspFNI CGCG 2 cut(s) 408, 539
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 3 cut(s) 116, 772, 807
BspMI ACCTGC 2 cut(s) 227, 1207
BspPI GGATC 5 cut(s) 137, 206, 280, 640, 741
BspQI GCTCTTC 2 cut(s) 19, 944
BspT107I GGYRCC 1 cut(s) 114
BspTNI GGTCTC 1 cut(s) 358
BsrBI CCGCTC 1 cut(s) 312
BssECI CCNNGG 4 cut(s) 289, 341, 1077, 1311
BssMI GATC 7 cut(s) 142, 198, 285, 444, 632, 679, 733
BssNI GRCGYC 2 cut(s) 192, 321
BssT1I CCWWGG 3 cut(s) 289, 1077, 1311
Bst2UI CCWGG 2 cut(s) 13, 196
Bst4CI ACNGT 2 cut(s) 1133, 1298
Bst6I CTCTTC 3 cut(s) 19, 691, 944
BstACI GRCGYC 2 cut(s) 192, 321
BstBAI YACGTR 1 cut(s) 278
BstC8I GCNNGC 3 cut(s) 31, 470, 1001
BstDEI CTNAG 1 cut(s) 1254
BstDSI CCRYGG 1 cut(s) 1311
BstEII GGTNACC 1 cut(s) 226
BstENI CCTNNNNNAGG 1 cut(s) 175
BstF5I GGATG 6 cut(s) 87, 171, 330, 600, 1229, 1307
BstFNI CGCG 2 cut(s) 408, 539
BstHHI GCGC 2 cut(s) 474, 1190
BstKTI GATC 7 cut(s) 145, 201, 288, 447, 635, 682, 736
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 7 cut(s) 142, 198, 285, 444, 632, 679, 733
BstMWI GCNNNNNNNGC 4 cut(s) 318, 327, 779, 833
BstNI CCWGG 2 cut(s) 13, 196
BstNSI RCATGY 1 cut(s) 746
BstPI GGTNACC 1 cut(s) 226
BstSCI CCNGG 3 cut(s) 11, 194, 222
BstSLI GKGCMC 2 cut(s) 119, 809
BstSNI TACGTA 1 cut(s) 278
BstUI CGCG 2 cut(s) 408, 539
BstV1I GCAGC 2 cut(s) 497, 563
BstV2I GAAGAC 1 cut(s) 844
BstX2I RGATCY 3 cut(s) 142, 632, 679
BstYI RGATCY 3 cut(s) 142, 632, 679
BsuRI GGCC 6 cut(s) 440, 618, 807, 1019, 1076, 1338
BtgI CCRYGG 1 cut(s) 1311
BtsCI GGATG 6 cut(s) 87, 171, 330, 600, 1229, 1307
BveI ACCTGC 2 cut(s) 227, 1207
Cac8I GCNNGC 3 cut(s) 31, 470, 1001
CciI TCATGA 1 cut(s) 135
CfoI GCGC 2 cut(s) 474, 1190
Cfr13I GGNCC 3 cut(s) 180, 805, 806
CseI GACGC 3 cut(s) 200, 289, 310
Csp6I GTAC 1 cut(s) 361
CspCI CAANNNNNGTGG 2 cut(s) 879, 914
CviAII CATG 8 cut(s) 76, 136, 591, 743, 846, 859, 1039, 1312
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 1 cut(s) 1254
DpnI GATC 7 cut(s) 144, 200, 287, 446, 634, 681, 735
DpnII GATC 7 cut(s) 142, 198, 285, 444, 632, 679, 733
EaeI YGGCCR 1 cut(s) 438
Eam1104I CTCTTC 3 cut(s) 19, 691, 944
EarI CTCTTC 3 cut(s) 19, 691, 944
Eco105I TACGTA 1 cut(s) 278
Eco130I CCWWGG 3 cut(s) 289, 1077, 1311
Eco147I AGGCCT 1 cut(s) 1019
Eco24I GRGCYC 1 cut(s) 809
Eco31I GGTCTC 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 180
Eco91I GGTNACC 1 cut(s) 226
EcoNI CCTNNNNNAGG 1 cut(s) 175
EcoO109I RGGNCCY 2 cut(s) 805, 806
EcoO65I GGTNACC 1 cut(s) 226
EcoRI GAATTC 1 cut(s) 1027
EcoRII CCWGG 2 cut(s) 11, 194
EcoT14I CCWWGG 3 cut(s) 289, 1077, 1311
EcoT38I GRGCYC 1 cut(s) 809
ErhI CCWWGG 3 cut(s) 289, 1077, 1311
FaeI CATG 8 cut(s) 79, 139, 594, 746, 849, 862, 1042, 1315
FatI CATG 8 cut(s) 75, 135, 590, 742, 845, 858, 1038, 1311
FblI GTMKAC 1 cut(s) 158
Fnu4HI GCNGC 3 cut(s) 409, 511, 552
FokI GGATG 6 cut(s) 94, 178, 317, 607, 1236, 1294
FriOI GRGCYC 1 cut(s) 809
Fsp4HI GCNGC 3 cut(s) 409, 511, 552
FspBI CTAG 4 cut(s) 71, 372, 810, 926
GlaI GCGC 2 cut(s) 473, 1189
GluI GCNGC 3 cut(s) 409, 511, 552
GsuI CTGGAG 1 cut(s) 34
HaeIII GGCC 6 cut(s) 440, 618, 807, 1019, 1076, 1338
HapII CCGG 5 cut(s) 60, 112, 146, 224, 350
HgaI GACGC 3 cut(s) 200, 289, 310
HhaI GCGC 2 cut(s) 474, 1190
Hin1I GRCGYC 2 cut(s) 192, 321
Hin1II CATG 8 cut(s) 79, 139, 594, 746, 849, 862, 1042, 1315
Hin6I GCGC 2 cut(s) 472, 1188
HinP1I GCGC 2 cut(s) 472, 1188
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 6 cut(s) 67, 97, 346, 400, 1162, 1304
HpaII CCGG 5 cut(s) 60, 112, 146, 224, 350
HphI GGTGA 6 cut(s) 220, 620, 799, 1145, 1151, 1241
Hpy166II GTNNAC 1 cut(s) 159
Hpy188I TCNGA 6 cut(s) 345, 384, 449, 603, 1167, 1197
Hpy188III TCNNGA 6 cut(s) 128, 136, 202, 304, 867, 891
Hpy8I GTNNAC 1 cut(s) 159
Hpy99I CGWCG 4 cut(s) 163, 250, 326, 559
HpyAV CCTTC 5 cut(s) 43, 111, 559, 608, 761
HpyCH4III ACNGT 2 cut(s) 1133, 1298
HpyCH4IV ACGT 3 cut(s) 248, 277, 557
HpyCH4V TGCA 5 cut(s) 760, 944, 999, 1119, 1216
HpyF10VI GCNNNNNNNGC 4 cut(s) 318, 327, 779, 833
HpyF3I CTNAG 1 cut(s) 1254
HpySE526I ACGT 3 cut(s) 248, 277, 557
Hsp92I GRCGYC 2 cut(s) 192, 321
Hsp92II CATG 8 cut(s) 79, 139, 594, 746, 849, 862, 1042, 1315
HspAI GCGC 2 cut(s) 472, 1188
Kzo9I GATC 7 cut(s) 142, 198, 285, 444, 632, 679, 733
LguI GCTCTTC 2 cut(s) 19, 944
LmnI GCTCC 2 cut(s) 770, 869
Lsp1109I GCAGC 2 cut(s) 497, 563
LweI GCATC 2 cut(s) 339, 931
MaeI CTAG 4 cut(s) 71, 372, 810, 926
MaeII ACGT 3 cut(s) 248, 277, 557
MaeIII GTNAC 6 cut(s) 148, 209, 226, 367, 397, 1249
MalI GATC 7 cut(s) 144, 200, 287, 446, 634, 681, 735
MbiI CCGCTC 1 cut(s) 312
MboI GATC 7 cut(s) 142, 198, 285, 444, 632, 679, 733
MflI RGATCY 3 cut(s) 142, 632, 679
MhlI GDGCHC 2 cut(s) 119, 809
MlsI TGGCCA 1 cut(s) 440
MluCI AATT 7 cut(s) 431, 795, 902, 1027, 1104, 1289, 1340
MluNI TGGCCA 1 cut(s) 440
MlyI GAGTC 1 cut(s) 394
MmeI TCCRAC 1 cut(s) 1201
Mox20I TGGCCA 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 1268
MscI TGGCCA 1 cut(s) 440
MseI TTAA 3 cut(s) 1053, 1064, 1320
MslI CAYNNNNRTG 2 cut(s) 80, 747
Msp20I TGGCCA 1 cut(s) 440
MspI CCGG 5 cut(s) 60, 112, 146, 224, 350
MspR9I CCNGG 3 cut(s) 13, 196, 224
MvaI CCWGG 2 cut(s) 13, 196
MvnI CGCG 2 cut(s) 408, 539
MwoI GCNNNNNNNGC 4 cut(s) 318, 327, 779, 833
NciI CCSGG 1 cut(s) 224
NcoI CCATGG 1 cut(s) 1311
NdeII GATC 7 cut(s) 142, 198, 285, 444, 632, 679, 733
NlaIII CATG 8 cut(s) 79, 139, 594, 746, 849, 862, 1042, 1315
NlaIV GGNNCC 3 cut(s) 116, 772, 807
NmuCI GTSAC 5 cut(s) 148, 209, 226, 367, 397
NspI RCATGY 1 cut(s) 746
PagI TCATGA 1 cut(s) 135
PaqCI CACCTGC 1 cut(s) 227
PceI AGGCCT 1 cut(s) 1019
PciI ACATGT 1 cut(s) 742
PciSI GCTCTTC 2 cut(s) 19, 944
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PdmI GAANNNNTTC 1 cut(s) 1268
PfeI GAWTC 5 cut(s) 67, 97, 346, 1162, 1304
Pfl23II CGTACG 1 cut(s) 360
PfoI TCCNGGA 1 cut(s) 11
PkrI GCNGC 3 cut(s) 410, 512, 553
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Ppu21I YACGTR 1 cut(s) 278
PscI ACATGT 1 cut(s) 742
Psp6I CCWGG 2 cut(s) 11, 194
PspEI GGTNACC 1 cut(s) 226
PspGI CCWGG 2 cut(s) 11, 194
PspLI CGTACG 1 cut(s) 360
PspN4I GGNNCC 3 cut(s) 116, 772, 807
PspOMI GGGCCC 1 cut(s) 805
PspPI GGNCC 3 cut(s) 180, 805, 806
PsuI RGATCY 3 cut(s) 142, 632, 679
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
RseI CAYNNNNRTG 2 cut(s) 80, 747
SalI GTCGAC 1 cut(s) 157
SapI GCTCTTC 2 cut(s) 19, 944
SaqAI TTAA 3 cut(s) 1053, 1064, 1320
SatI GCNGC 3 cut(s) 409, 511, 552
Sau3AI GATC 7 cut(s) 142, 198, 285, 444, 632, 679, 733
Sau96I GGNCC 3 cut(s) 180, 805, 806
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 3 cut(s) 13, 196, 224
SduI GDGCHC 2 cut(s) 119, 809
SfaNI GCATC 2 cut(s) 339, 931
SinI GGWCC 1 cut(s) 180
SmiMI CAYNNNNRTG 2 cut(s) 80, 747
SnaBI TACGTA 1 cut(s) 278
Sse9I AATT 7 cut(s) 431, 795, 902, 1027, 1104, 1289, 1340
SseBI AGGCCT 1 cut(s) 1019
SsiI CCGC 3 cut(s) 310, 408, 539
SspMI CTAG 4 cut(s) 71, 372, 810, 926
StuI AGGCCT 1 cut(s) 1019
StyD4I CCNGG 3 cut(s) 11, 194, 222
StyI CCWWGG 3 cut(s) 289, 1077, 1311
TaaI ACNGT 2 cut(s) 1133, 1298
TaiI ACGT 3 cut(s) 251, 280, 560
TaqI TCGA 6 cut(s) 129, 158, 201, 252, 324, 528
TasI AATT 7 cut(s) 431, 795, 902, 1027, 1104, 1289, 1340
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 5 cut(s) 67, 97, 346, 1162, 1304
Tru1I TTAA 3 cut(s) 1053, 1064, 1320
Tru9I TTAA 3 cut(s) 1053, 1064, 1320
TseFI GTSAC 5 cut(s) 148, 209, 226, 367, 397
TseI GCWGC 2 cut(s) 510, 551
Tsp45I GTSAC 5 cut(s) 148, 209, 226, 367, 397
TspDTI ATGAA 4 cut(s) 152, 894, 1248, 1296
TspGWI ACGGA 1 cut(s) 571
VpaK11BI GGWCC 1 cut(s) 180
XagI CCTNNNNNAGG 1 cut(s) 175
XapI RAATTY 1 cut(s) 1027
XceI RCATGY 1 cut(s) 746
XcmI CCANNNNNNNNNTGG 5 cut(s) 238, 626, 781, 1045, 1133
XmiI GTMKAC 1 cut(s) 158
XmnI GAANNNNTTC 1 cut(s) 1268
XspI CTAG 4 cut(s) 71, 372, 810, 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.