pycom06g20780

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001443
Physical Location & Seq
Reverse (-)
23668855 .. 23670198
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g20780.3

Sequence Viewer

Length: 1245 bp
ATGGGTTTAGGCTGGAGAAGGAGGAGAACCGGCCACCGCGCCGCGAAAAACCAGCCAGGGGACCTCGACATGGAAGTCCTGATCCCGGACCATTTTCTCTGCCCTATATCGCTTGATTTGATGAAGATCATTTTGATCCGGTCACATTGTGGGAACTTTACTTGCCCTGTGACAAATCAGGTGTTGAGAAGGTTCGATCAGATTCCCAACCATAGTCTCAGAAAAATGATTCAAGAATGGTGCACAGAAAACAAGCGCTACGGAATTGAACGTGTTCCAACGCCGCGAGTTCCTGTTATGCCGATCGAGGTCTCCGAGAATCTTTTCAGTTTAGCGGGCTCAGCTCGAAGTTTGGATCACCAAGGGTGCTTCGAATGTGTGCGCAGAATCAAGAATTGGGCCGCTGAGAGTGAGCGAAACAAGCGGTGCATATTGGAGAACGGGGCTGCATCTGTACTCTCTGCCGCCTTTGATTCTTTTGCGGGCGATTGTGAAAAGAAAAATGCAGTTGTGTGCGAAGAATTACTCTCTGCGTTGAGTTGGATGCTTCCAGCTTTCGATGAAGAATCACACAAATACTTGGGATCGAAGGCATCATTGCGTTGCATGGTTTGGTTTCTCAAGGAAACCGATGATCTTTCGGTCAAGCAAAACTCCCTTCTAGTATTGAAACAGCTTCTTTCTTGCGATGATCACACTAAACATGCTGAAGCGTTGGCGGAAATTGGTGGGGTGAATGACATTCTGTTCAAGCTCATCAGAGAGAAAATTTCGCCGGCGATTACCAAGGCATCTCTGATGGTATTATCATATTTGGAGATGGGATTGGTTTCTGTGTTGCTGGAAATGCTTGTGGACTCGGAAAGGGGCGTAAGCGAGGGGGCATTGGCGGTTATCGACAGTCTTTGTGATTGCGAAGAAGGGAGGGAAAAGGCGTACGCTGATGCGCTAACCATACCGGTTTTAGTGAAGAAAATTTTGAGGATATCGGAAATGGCGACTGAGTATTCGATTTCAGCGATTTGGAAGCTGTGTAAATGTGCAAGCAGGCAAGAAGAAAGAGTGCTGGTTGAGGCCCTTCAAGTTGGTACATTTCACAAGCTCTTGTTGGTTTTACAGGTTCGTTGCGGGGACGATCACACGAAGGAGAAGACGAACGAGCTTCTGAAGCTCTTGAATCCTTACAGAGCTGGATTGGAATGCATTGAGTCTGTAGATTTCAAGAGTATTAGAAGGTCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

415

Amino Acids

46.67

Weight (kDa)

6.8

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
Acc16I TGCGCA 1 cut(s) 383
AccII CGCG 3 cut(s) 39, 44, 286
AclWI GGATC 4 cut(s) 76, 130, 363, 592
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 2 cut(s) 768, 975
AcuI CTGAAG 2 cut(s) 729, 1187
AdeI CACNNNGTG 1 cut(s) 149
AfaI GTAC 3 cut(s) 456, 938, 1090
AfeI AGCGCT 1 cut(s) 257
AfiI CCNNNNNNNGG 3 cut(s) 58, 70, 85
AflIII ACRYGT 1 cut(s) 271
AgeI ACCGGT 1 cut(s) 958
AgsI TTSAA 7 cut(s) 233, 269, 670, 751, 1082, 1177, 1222
AjnI CCWGG 1 cut(s) 55
AjuI GAANNNNNNNTTGG 2 cut(s) 379, 411
AluBI AGCT 9 cut(s) 344, 554, 676, 754, 1030, 1102, 1162, 1171, 1190
AluI AGCT 9 cut(s) 344, 554, 676, 754, 1030, 1102, 1162, 1171, 1190
Alw21I GWGCWC 1 cut(s) 245
Alw26I GTCTC 2 cut(s) 221, 316
Alw44I GTGCAC 1 cut(s) 241
AlwI GGATC 4 cut(s) 76, 130, 363, 592
Aor51HI AGCGCT 1 cut(s) 257
AoxI GGCC 3 cut(s) 31, 399, 1074
ApaLI GTGCAC 1 cut(s) 241
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 2 cut(s) 768, 975
ArsI GACNNNNNNTTYG 2 cut(s) 991, 1023
AsiGI ACCGGT 1 cut(s) 958
Asp700I GAANNNNTTC 2 cut(s) 273, 323
AspLEI GCGC 4 cut(s) 41, 258, 384, 949
AspS9I GGNCC 4 cut(s) 61, 88, 399, 1075
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 2 cut(s) 350, 745
AsuII TTCGAA 1 cut(s) 372
AvaII GGWCC 2 cut(s) 61, 88
BaeGI GKGCMC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 341
BbsI GAAGAC 1 cut(s) 1157
Bbv12I GWGCWC 1 cut(s) 245
BbvI GCAGC 1 cut(s) 433
BccI CCATC 2 cut(s) 793, 814
BciT130I CCWGG 1 cut(s) 57
BclI TGATCA 1 cut(s) 691
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 2 cut(s) 221, 316
BfaI CTAG 1 cut(s) 662
BfmI CTRYAG 1 cut(s) 1212
BfoI RGCGCY 1 cut(s) 259
BisI GCNGC 5 cut(s) 42, 284, 402, 447, 465
BlpI GCTNAGC 1 cut(s) 340
BlsI GCNGC 5 cut(s) 43, 285, 403, 448, 466
Bme1390I CCNGG 2 cut(s) 57, 86
Bme18I GGWCC 2 cut(s) 61, 88
BmgT120I GGNCC 4 cut(s) 61, 88, 399, 1075
BmiI GGNNCC 1 cut(s) 62
BmrFI CCNGG 2 cut(s) 57, 86
BmsI GCATC 5 cut(s) 458, 534, 602, 800, 934
BpiI GAAGAC 1 cut(s) 1157
BpmI CTGGAG 1 cut(s) 34
Bpu1102I GCTNAGC 1 cut(s) 340
Bpu14I TTCGAA 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 125
BsaI GGTCTC 1 cut(s) 316
BsaJI CCNNGG 3 cut(s) 56, 361, 786
BsaWI WCCGGW 2 cut(s) 138, 958
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 70, 85
Bse118I RCCGGY 3 cut(s) 29, 775, 958
Bse3DI GCAATG 1 cut(s) 596
Bse8I GATNNNNATC 1 cut(s) 125
BseBI CCWGG 1 cut(s) 57
BseDI CCNNGG 3 cut(s) 56, 361, 786
BseGI GGATG 1 cut(s) 549
BseJI GATNNNNATC 1 cut(s) 125
BseLI CCNNNNNNNGG 3 cut(s) 58, 70, 85
BseMI GCAATG 1 cut(s) 596
BseMII CTCAG 4 cut(s) 232, 354, 396, 993
BseRI GAGGAG 1 cut(s) 37
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 1 cut(s) 433
Bsh1236I CGCG 3 cut(s) 39, 44, 286
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 3 cut(s) 33, 401, 1076
BshTI ACCGGT 1 cut(s) 958
BsiEI CGRYCG 1 cut(s) 306
BsiHKAI GWGCWC 1 cut(s) 245
BsiSI CCGG 5 cut(s) 30, 86, 139, 776, 959
BsiWI CGTACG 1 cut(s) 936
BslFI GGGAC 2 cut(s) 74, 1145
BslI CCNNNNNNNGG 3 cut(s) 58, 70, 85
BsmAI GTCTC 2 cut(s) 221, 316
BsmFI GGGAC 2 cut(s) 74, 1145
BsmI GAATGC 1 cut(s) 1205
BsnI GGCC 3 cut(s) 33, 401, 1076
Bso31I GGTCTC 1 cut(s) 316
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 2 cut(s) 245, 341
Bsp1720I GCTNAGC 1 cut(s) 340
BspANI GGCC 3 cut(s) 33, 401, 1076
BspCNI CTCAG 4 cut(s) 231, 353, 397, 994
BspFNI CGCG 3 cut(s) 39, 44, 286
BspLI GGNNCC 1 cut(s) 62
BspPI GGATC 4 cut(s) 76, 130, 363, 592
BspT104I TTCGAA 1 cut(s) 372
BspTNI GGTCTC 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 596
BsrFI RCCGGY 3 cut(s) 29, 775, 958
BssAI RCCGGY 3 cut(s) 29, 775, 958
BssECI CCNNGG 3 cut(s) 56, 361, 786
BssT1I CCWWGG 2 cut(s) 361, 786
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 902
BstBI TTCGAA 1 cut(s) 372
BstC8I GCNNGC 5 cut(s) 337, 484, 777, 1045, 1049
BstDEI CTNAG 4 cut(s) 218, 340, 405, 1002
BstF5I GGATG 1 cut(s) 549
BstFNI CGCG 3 cut(s) 39, 44, 286
BstH2I RGCGCY 1 cut(s) 259
BstHHI GCGC 4 cut(s) 41, 258, 384, 949
BstMAI GTCTC 2 cut(s) 221, 316
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 4 cut(s) 341, 421, 847, 1168
BstNI CCWGG 1 cut(s) 57
BstNSI RCATGY 1 cut(s) 707
BstSCI CCNGG 2 cut(s) 55, 84
BstSFI CTRYAG 1 cut(s) 1212
BstSLI GKGCMC 1 cut(s) 245
BstUI CGCG 3 cut(s) 39, 44, 286
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 1 cut(s) 1157
BsuRI GGCC 3 cut(s) 33, 401, 1076
BtgZI GCGATG 1 cut(s) 702
BtsCI GGATG 1 cut(s) 549
Cac8I GCNNGC 5 cut(s) 337, 484, 777, 1045, 1049
CfoI GCGC 4 cut(s) 41, 258, 384, 949
Cfr10I RCCGGY 3 cut(s) 29, 775, 958
Cfr13I GGNCC 4 cut(s) 61, 88, 399, 1075
Csp6I GTAC 3 cut(s) 455, 937, 1089
CspAI ACCGGT 1 cut(s) 958
CviAII CATG 3 cut(s) 70, 607, 704
CviQI GTAC 3 cut(s) 455, 937, 1089
DdeI CTNAG 4 cut(s) 218, 340, 405, 1002
DraIII CACNNNGTG 1 cut(s) 149
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 31
EciI GGCGGA 1 cut(s) 734
Eco130I CCWWGG 2 cut(s) 361, 786
Eco24I GRGCYC 1 cut(s) 341
Eco31I GGTCTC 1 cut(s) 316
Eco32I GATATC 1 cut(s) 987
Eco47I GGWCC 2 cut(s) 61, 88
Eco47III AGCGCT 1 cut(s) 257
Eco57I CTGAAG 2 cut(s) 729, 1187
EcoO109I RGGNCCY 2 cut(s) 61, 1075
EcoRII CCWGG 1 cut(s) 55
EcoRV GATATC 1 cut(s) 987
EcoT14I CCWWGG 2 cut(s) 361, 786
EcoT22I ATGCAT 1 cut(s) 1205
EcoT38I GRGCYC 1 cut(s) 341
ErhI CCWWGG 2 cut(s) 361, 786
FaeI CATG 3 cut(s) 73, 610, 707
FaiI YATR 9 cut(s) 71, 107, 213, 299, 431, 608, 705, 811, 956
FaqI GGGAC 2 cut(s) 74, 1145
FatI CATG 3 cut(s) 69, 606, 703
FauI CCCGC 3 cut(s) 328, 475, 1121
FbaI TGATCA 1 cut(s) 691
Fnu4HI GCNGC 5 cut(s) 42, 284, 402, 447, 465
FokI GGATG 1 cut(s) 556
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 5 cut(s) 42, 284, 402, 447, 465
FspBI CTAG 1 cut(s) 662
FspI TGCGCA 1 cut(s) 383
GlaI GCGC 4 cut(s) 40, 257, 383, 948
GluI GCNGC 5 cut(s) 42, 284, 402, 447, 465
GsuI CTGGAG 1 cut(s) 34
HaeII RGCGCY 1 cut(s) 259
HaeIII GGCC 3 cut(s) 33, 401, 1076
HapII CCGG 5 cut(s) 30, 86, 139, 776, 959
HhaI GCGC 4 cut(s) 41, 258, 384, 949
Hin1II CATG 3 cut(s) 73, 610, 707
Hin6I GCGC 4 cut(s) 39, 256, 382, 947
HinP1I GCGC 4 cut(s) 39, 256, 382, 947
HinfI GANTC 9 cut(s) 202, 229, 319, 387, 473, 566, 857, 1177, 1208
HpaII CCGG 5 cut(s) 30, 86, 139, 776, 959
HphI GGTGA 2 cut(s) 350, 745
Hpy166II GTNNAC 2 cut(s) 243, 856
Hpy188I TCNGA 8 cut(s) 201, 221, 316, 761, 798, 862, 991, 1167
Hpy188III TCNNGA 5 cut(s) 79, 233, 391, 1174, 1222
Hpy8I GTNNAC 2 cut(s) 243, 856
HpyAV CCTTC 8 cut(s) 12, 183, 583, 668, 914, 1088, 1138, 1227
HpyCH4III ACNGT 1 cut(s) 902
HpyCH4IV ACGT 1 cut(s) 271
HpyCH4V TGCA 7 cut(s) 243, 429, 449, 506, 606, 1043, 1203
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 421, 847, 1168
HpyF3I CTNAG 4 cut(s) 218, 340, 405, 1002
HpySE526I ACGT 1 cut(s) 271
Hsp92II CATG 3 cut(s) 73, 610, 707
HspAI GCGC 4 cut(s) 39, 256, 382, 947
KroI GCCGGC 1 cut(s) 775
KroNI GCCGGC 1 cut(s) 777
Ksp22I TGATCA 1 cut(s) 691
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 5 cut(s) 458, 534, 602, 800, 934
MaeI CTAG 1 cut(s) 662
MaeII ACGT 1 cut(s) 271
MaeIII GTNAC 2 cut(s) 141, 169
MboII GAAGA 7 cut(s) 136, 530, 575, 929, 982, 1067, 1162
MhlI GDGCHC 2 cut(s) 245, 341
MluCI AATT 6 cut(s) 264, 394, 521, 723, 768, 975
MlyI GAGTC 2 cut(s) 851, 1217
MmeI TCCRAC 2 cut(s) 302, 521
MnlI CCTC 7 cut(s) 15, 74, 301, 871, 918, 975, 1066
Mph1103I ATGCAT 1 cut(s) 1205
MreI CGCCGGCG 1 cut(s) 775
MroNI GCCGGC 1 cut(s) 775
MroXI GAANNNNTTC 2 cut(s) 273, 323
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 5 cut(s) 30, 86, 139, 776, 959
MspR9I CCNGG 2 cut(s) 57, 86
Mva1269I GAATGC 1 cut(s) 1205
MvaI CCWGG 1 cut(s) 57
MvnI CGCG 3 cut(s) 39, 44, 286
MwoI GCNNNNNNNGC 4 cut(s) 341, 421, 847, 1168
NaeI GCCGGC 1 cut(s) 777
NciI CCSGG 1 cut(s) 86
NgoMIV GCCGGC 1 cut(s) 775
NlaIII CATG 3 cut(s) 73, 610, 707
NlaIV GGNNCC 1 cut(s) 62
NmuCI GTSAC 2 cut(s) 141, 169
NsbI TGCGCA 1 cut(s) 383
NsiI ATGCAT 1 cut(s) 1205
NspI RCATGY 1 cut(s) 707
NspV TTCGAA 1 cut(s) 372
PcsI WCGNNNNNNNCGW 3 cut(s) 312, 995, 1016
PctI GAATGC 1 cut(s) 1205
PdiI GCCGGC 1 cut(s) 777
PdmI GAANNNNTTC 2 cut(s) 273, 323
PfeI GAWTC 7 cut(s) 202, 229, 319, 387, 473, 566, 1177
Pfl23II CGTACG 1 cut(s) 936
PfoI TCCNGGA 1 cut(s) 84
PinAI ACCGGT 1 cut(s) 958
PkrI GCNGC 5 cut(s) 43, 285, 403, 448, 466
Ple19I CGATCG 1 cut(s) 306
PleI GAGTC 2 cut(s) 851, 1216
PpsI GAGTC 2 cut(s) 851, 1216
PpuMI RGGWCCY 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 61
Psp6I CCWGG 1 cut(s) 55
PspGI CCWGG 1 cut(s) 55
PspLI CGTACG 1 cut(s) 936
PspN4I GGNNCC 1 cut(s) 62
PspPI GGNCC 4 cut(s) 61, 88, 399, 1075
PspPPI RGGWCCY 1 cut(s) 61
PvuI CGATCG 1 cut(s) 306
RsaI GTAC 3 cut(s) 456, 938, 1090
RsaNI GTAC 3 cut(s) 455, 937, 1089
SatI GCNGC 5 cut(s) 42, 284, 402, 447, 465
Sau96I GGNCC 4 cut(s) 61, 88, 399, 1075
SchI GAGTC 2 cut(s) 851, 1217
ScrFI CCNGG 2 cut(s) 57, 86
SduI GDGCHC 2 cut(s) 245, 341
SfaNI GCATC 5 cut(s) 458, 534, 602, 800, 934
SfcI CTRYAG 1 cut(s) 1212
SfuI TTCGAA 1 cut(s) 372
SgrAI CRCCGGYG 1 cut(s) 775
SinI GGWCC 2 cut(s) 61, 88
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
Sse9I AATT 6 cut(s) 264, 394, 521, 723, 768, 975
SspMI CTAG 1 cut(s) 662
StyD4I CCNGG 2 cut(s) 55, 84
StyI CCWWGG 2 cut(s) 361, 786
TaaI ACNGT 1 cut(s) 902
TaiI ACGT 1 cut(s) 274
TaqI TCGA 9 cut(s) 66, 195, 306, 346, 372, 558, 587, 897, 1010
TaqII GACCGA 1 cut(s) 631
TasI AATT 6 cut(s) 264, 394, 521, 723, 768, 975
TatI WGTACW 1 cut(s) 454
TauI GCSGC 4 cut(s) 44, 286, 404, 467
TfiI GAWTC 7 cut(s) 202, 229, 319, 387, 473, 566, 1177
TseFI GTSAC 2 cut(s) 141, 169
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 2 cut(s) 141, 169
TspDTI ATGAA 2 cut(s) 137, 576
TspGWI ACGGA 1 cut(s) 276
VneI GTGCAC 1 cut(s) 241
VpaK11BI GGWCC 2 cut(s) 61, 88
XapI RAATTY 2 cut(s) 768, 975
XceI RCATGY 1 cut(s) 707
XmnI GAANNNNTTC 2 cut(s) 273, 323
XspI CTAG 1 cut(s) 662
Zsp2I ATGCAT 1 cut(s) 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.