RLG00000006019

U-box domain-containing protein 21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001443
Physical Location & Seq
Reverse (-)
3588765 .. 3590602
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006019

Sequence Viewer

Length: 1341 bp
ATGACTTTGTCATGGAGAAGGTTGAGGCCCGGCCGTCACGCCGCGAAACATTCACGGCATGGCGAGGCCGGCGGCGACATTGAGCTGACGATACCGAGCCACTTCCGGTGCCCGATCTCGTTGGACTTGATGAAAGATCCCGTCACGTTGTCTACCGGGATCACATACGATCGGCAGAGCATTGAGAAATGGATCGAAGCCGGAAACGTGACTTGCCCTACTACGAACCAGGTACTCGCAACTTTGGAGCCGACTCCGAATCACAACATCAGGAAAATGATACAAGACTGGTGCGTGGAGAAGAAGTCCTTCGGCGTGGAGCGTATCCCCACGCCCCGGATTCCGGTGAGTTCTGTGGAGGTCTCCGAGATTCTCTCGAGGATTACGTCGTCGGCGGAAAAGAAGGACGGAGCCGGCTGCAGAGAGCTGGTGCAAAAGGTCAAGGCGTTGACTAAAGAAAGCGAGCGCAACAAGCGGTGCATTGTCGCTAACGGAGCGGCGAGTGCTTTATCGGCGGCGTTTGGTGCTTTTTCAACAGCGGGTTCTTTTCATGAAAACGACGGCGTTTTGGAGGAGATCTTGTCTGCTCTGACGTTGATTTTCCCGCTTGATACGAAGGCCAACGTATATCTTGGATCCACAGATTCATTGCATTGCATGGTGAGGTTTTTGGACGGTGGAGATTTGTCACAGAGAAGGAACGCAGTCTTGGTTCTAAAAGAGGCTCTTTCAGCTGATAAGCAAAAGACTGATGCTTTGGAAGAGATTGAAGGAGCTTTGGAAGCCCTAGTGAAGCTGATCAAAGAGCCAATTTGCCCTACTTCTACTAAAGCTTCGTTGGTCATCATTTACCACATGCTTAATTCCACGTCTTCTAGCTCAAGAGAGAACGTCAAAGGAAAATTTGTGCAAATGGGATTGGTGTCCATGCTATCGGAATTAGTTGTGGATGCCGAGAGGAGGATTTGCGAAAAGGCGTTGGGGGTTCTCGATGCGATTTGTGGAAGCAAGCAAGGGAGGGAAGAGGCCCGCGACAATGCCCTGACCATGCCGGTCGTAGTCAAGAAGATGCTTAGGGTTTCGGATTTAGCTACCGAGTTTGCTGTGTCCATTATTTGGAAGCTTTGCAAGAAGGACGAGAGGGAAGACGACGAGGGAGGTCTTGTTCTAGAGGTGCTTCAAGTGGGAGCTTTTCAGAAGCTGTTGTTGCTGTTGCAAGTTGGGTGTGGGGAGAGGACTAAAGAGAAGGCGACCGAGTTGTTGAAGTTGTTGAATCTTCACAGGGCGAGGTTGGAGTGCATTGAGTCCATGGATTTTAAGGATCTCAAGAGGCCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

447

Amino Acids

49.22

Weight (kDa)

7.92

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 31 - 102 9.4e-21 U-box domain
ARM_PUB PF25598 120 - 441 4e-73 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 108
AccB7I CCANNNNNTGG 1 cut(s) 1116
AccBSI CCGCTC 1 cut(s) 497
AccI GTMKAC 1 cut(s) 152
AccII CGCG 2 cut(s) 44, 1032
AciI CCGC 9 cut(s) 42, 72, 395, 475, 497, 515, 539, 605, 1030
AclWI GGATC 6 cut(s) 131, 167, 200, 630, 643, 1329
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 1 cut(s) 902
AfaI GTAC 1 cut(s) 234
AfiI CCNNNNNNNGG 4 cut(s) 336, 343, 960, 1116
AgsI TTSAA 5 cut(s) 534, 770, 1181, 1264, 1273
AjiI CACGTC 1 cut(s) 870
AjnI CCWGG 1 cut(s) 228
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AloI GAACNNNNNNTCC 2 cut(s) 1149, 1181
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 6 cut(s) 131, 167, 200, 630, 643, 1329
AlwNI CAGNNNCTG 1 cut(s) 1201
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 6 cut(s) 26, 31, 66, 618, 1026, 1331
ApeKI GCWGC 1 cut(s) 417
ApoI RAATTY 1 cut(s) 902
ArsI GACNNNNNNTTYG 2 cut(s) 739, 771
Asp700I GAANNNNTTC 1 cut(s) 308
AspLEI GCGC 1 cut(s) 468
AspS9I GGNCC 3 cut(s) 27, 1027, 1332
AsuC2I CCSGG 3 cut(s) 30, 157, 337
AsuHPI GGTGA 2 cut(s) 358, 673
AvaI CYCGRG 1 cut(s) 376
BaeGI GKGCMC 1 cut(s) 113
BamHI GGATCC 1 cut(s) 635
BanI GGYRCC 1 cut(s) 108
BbsI GAAGAC 2 cut(s) 864, 1152
BbvI GCAGC 1 cut(s) 404
BceAI ACGGC 3 cut(s) 18, 71, 577
BciT130I CCWGG 1 cut(s) 230
BciVI GTATCC 1 cut(s) 335
BclI TGATCA 1 cut(s) 798
BcnI CCSGG 3 cut(s) 30, 157, 337
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 3 cut(s) 788, 876, 1169
BfmI CTRYAG 1 cut(s) 418
BfuI GTATCC 1 cut(s) 335
BglII AGATCT 1 cut(s) 576
BisI GCNGC 5 cut(s) 42, 73, 418, 498, 516
BlsI GCNGC 5 cut(s) 43, 74, 419, 499, 517
Bme1390I CCNGG 4 cut(s) 30, 157, 230, 337
BmeT110I CYCGRG 1 cut(s) 376
BmgBI CACGTC 1 cut(s) 870
BmgT120I GGNCC 3 cut(s) 27, 1027, 1332
BmiI GGNNCC 4 cut(s) 110, 249, 412, 637
BmrFI CCNGG 4 cut(s) 30, 157, 230, 337
BmsI GCATC 4 cut(s) 742, 940, 982, 1059
BpiI GAAGAC 2 cut(s) 864, 1152
BplI GAGNNNNNCTC 2 cut(s) 359, 391
Bpu10I CCTNAGC 1 cut(s) 1073
BpuEI CTTGAG 2 cut(s) 865, 1310
BpuMI CCSGG 3 cut(s) 30, 157, 337
BsaI GGTCTC 1 cut(s) 367
BsaJI CCNNGG 2 cut(s) 335, 1308
BsaWI WCCGGW 2 cut(s) 105, 343
BsaXI ACNNNNNCTCC 6 cut(s) 290, 320, 566, 596, 1149, 1179
Bsc4I CCNNNNNNNGG 4 cut(s) 336, 343, 960, 1116
Bse118I RCCGGY 3 cut(s) 68, 413, 1051
Bse1I ACTGG 1 cut(s) 293
Bse3DI GCAATG 2 cut(s) 647, 652
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 335, 1308
BseGI GGATG 1 cut(s) 955
BseLI CCNNNNNNNGG 4 cut(s) 336, 343, 960, 1116
BseMI GCAATG 2 cut(s) 647, 652
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 2 cut(s) 587, 973
BseSI GKGCMC 1 cut(s) 113
BseX3I CGGCCG 1 cut(s) 31
BseXI GCAGC 1 cut(s) 404
Bsh1236I CGCG 2 cut(s) 44, 1032
Bsh1285I CGRYCG 4 cut(s) 34, 172, 1056, 1254
BshFI GGCC 6 cut(s) 28, 33, 68, 620, 1028, 1333
BshNI GGYRCC 1 cut(s) 108
BsiEI CGRYCG 4 cut(s) 34, 172, 1056, 1254
BsiHKCI CYCGRG 1 cut(s) 376
BsiSI CCGG 9 cut(s) 30, 69, 106, 156, 201, 337, 344, 414, 1052
BslI CCNNNNNNNGG 4 cut(s) 336, 343, 960, 1116
BsmAI GTCTC 1 cut(s) 367
BsnI GGCC 6 cut(s) 28, 33, 68, 620, 1028, 1333
Bso31I GGTCTC 1 cut(s) 367
BsoBI CYCGRG 1 cut(s) 376
Bsp1286I GDGCHC 1 cut(s) 113
Bsp143I GATC 9 cut(s) 114, 136, 159, 169, 192, 576, 635, 798, 1321
Bsp19I CCATGG 1 cut(s) 1308
BspACI CCGC 9 cut(s) 42, 72, 395, 475, 497, 515, 539, 605, 1030
BspANI GGCC 6 cut(s) 28, 33, 68, 620, 1028, 1333
BspFNI CGCG 2 cut(s) 44, 1032
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 4 cut(s) 110, 249, 412, 637
BspMAI CTGCAG 1 cut(s) 422
BspPI GGATC 6 cut(s) 131, 167, 200, 630, 643, 1329
BspT107I GGYRCC 1 cut(s) 108
BspTNI GGTCTC 1 cut(s) 367
BsrBI CCGCTC 1 cut(s) 497
BsrDI GCAATG 2 cut(s) 647, 652
BsrFI RCCGGY 3 cut(s) 68, 413, 1051
BsrI ACTGG 1 cut(s) 293
BssAI RCCGGY 3 cut(s) 68, 413, 1051
BssECI CCNNGG 2 cut(s) 335, 1308
BssMI GATC 9 cut(s) 114, 136, 159, 169, 192, 576, 635, 798, 1321
BssT1I CCWWGG 1 cut(s) 1308
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 677
Bst6I CTCTTC 2 cut(s) 756, 1017
BstC8I GCNNGC 5 cut(s) 70, 415, 464, 1010, 1030
BstDEI CTNAG 1 cut(s) 1073
BstDSI CCRYGG 1 cut(s) 1308
BstF5I GGATG 1 cut(s) 955
BstFNI CGCG 2 cut(s) 44, 1032
BstHHI GCGC 1 cut(s) 468
BstKTI GATC 9 cut(s) 117, 139, 162, 172, 195, 579, 638, 801, 1324
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 9 cut(s) 114, 136, 159, 169, 192, 576, 635, 798, 1321
BstMCI CGRYCG 4 cut(s) 34, 172, 1056, 1254
BstMWI GCNNNNNNNGC 9 cut(s) 69, 472, 494, 503, 512, 524, 731, 782, 1207
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 859
BstSCI CCNGG 4 cut(s) 28, 155, 228, 335
BstSFI CTRYAG 1 cut(s) 418
BstSLI GKGCMC 1 cut(s) 113
BstUI CGCG 2 cut(s) 44, 1032
BstV1I GCAGC 1 cut(s) 404
BstV2I GAAGAC 2 cut(s) 864, 1152
BstX2I RGATCY 4 cut(s) 136, 576, 635, 1321
BstYI RGATCY 4 cut(s) 136, 576, 635, 1321
BstZI CGGCCG 1 cut(s) 31
BsuI GTATCC 1 cut(s) 335
BsuRI GGCC 6 cut(s) 28, 33, 68, 620, 1028, 1333
BtgI CCRYGG 1 cut(s) 1308
BtrI CACGTC 1 cut(s) 870
BtsCI GGATG 1 cut(s) 955
Cac8I GCNNGC 5 cut(s) 70, 415, 464, 1010, 1030
CaiI CAGNNNCTG 1 cut(s) 1201
CciI TCATGA 1 cut(s) 550
CfoI GCGC 1 cut(s) 468
Cfr10I RCCGGY 3 cut(s) 68, 413, 1051
Cfr13I GGNCC 3 cut(s) 27, 1027, 1332
CsiI ACCWGGT 1 cut(s) 228
Csp6I GTAC 1 cut(s) 233
CviAII CATG 8 cut(s) 12, 59, 551, 658, 856, 928, 1048, 1309
CviQI GTAC 1 cut(s) 233
DdeI CTNAG 1 cut(s) 1073
DpnI GATC 9 cut(s) 116, 138, 161, 171, 194, 578, 637, 800, 1323
DpnII GATC 9 cut(s) 114, 136, 159, 169, 192, 576, 635, 798, 1321
EaeI YGGCCR 1 cut(s) 31
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 2 cut(s) 756, 1017
EarI CTCTTC 2 cut(s) 756, 1017
EciI GGCGGA 1 cut(s) 410
EclXI CGGCCG 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 1308
Eco31I GGTCTC 1 cut(s) 367
Eco52I CGGCCG 1 cut(s) 31
Eco88I CYCGRG 1 cut(s) 376
EcoO109I RGGNCCY 1 cut(s) 1332
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 1308
ErhI CCWWGG 1 cut(s) 1308
FaeI CATG 8 cut(s) 15, 62, 554, 661, 859, 931, 1051, 1312
FalI AAGNNNNNCTT 5 cut(s) 293, 325, 711, 743, 1319
FatI CATG 8 cut(s) 11, 58, 550, 657, 855, 927, 1047, 1308
FauI CCCGC 3 cut(s) 532, 612, 1037
FbaI TGATCA 1 cut(s) 798
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 5 cut(s) 42, 73, 418, 498, 516
FokI GGATG 1 cut(s) 962
Fsp4HI GCNGC 5 cut(s) 42, 73, 418, 498, 516
FspBI CTAG 3 cut(s) 788, 876, 1169
GlaI GCGC 1 cut(s) 467
GluI GCNGC 5 cut(s) 42, 73, 418, 498, 516
HaeIII GGCC 6 cut(s) 28, 33, 68, 620, 1028, 1333
HapII CCGG 9 cut(s) 30, 69, 106, 156, 201, 337, 344, 414, 1052
HhaI GCGC 1 cut(s) 468
Hin1II CATG 8 cut(s) 15, 62, 554, 661, 859, 931, 1051, 1312
Hin6I GCGC 1 cut(s) 466
HinP1I GCGC 1 cut(s) 466
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HindIII AAGCTT 2 cut(s) 831, 1121
HinfI GANTC 7 cut(s) 253, 259, 340, 370, 644, 1273, 1304
HpaII CCGG 9 cut(s) 30, 69, 106, 156, 201, 337, 344, 414, 1052
HphI GGTGA 2 cut(s) 358, 673
Hpy166II GTNNAC 2 cut(s) 153, 450
Hpy188I TCNGA 6 cut(s) 258, 367, 591, 937, 1084, 1197
Hpy188III TCNNGA 8 cut(s) 271, 376, 551, 882, 989, 1063, 1169, 1327
Hpy8I GTNNAC 2 cut(s) 153, 450
Hpy99I CGWCG 4 cut(s) 391, 394, 563, 1154
HpyAV CCTTC 8 cut(s) 12, 319, 397, 610, 690, 764, 1126, 1240
HpyCH4III ACNGT 1 cut(s) 677
HpyCH4IV ACGT 7 cut(s) 146, 207, 386, 593, 624, 869, 891
HpyCH4V TGCA 9 cut(s) 420, 433, 480, 652, 657, 910, 1128, 1216, 1299
HpyF10VI GCNNNNNNNGC 9 cut(s) 69, 472, 494, 503, 512, 524, 731, 782, 1207
HpyF3I CTNAG 1 cut(s) 1073
HpySE526I ACGT 7 cut(s) 146, 207, 386, 593, 624, 869, 891
Hsp92II CATG 8 cut(s) 15, 62, 554, 661, 859, 931, 1051, 1312
HspAI GCGC 1 cut(s) 466
KroI GCCGGC 2 cut(s) 68, 413
KroNI GCCGGC 2 cut(s) 70, 415
Ksp22I TGATCA 1 cut(s) 798
Kzo9I GATC 9 cut(s) 114, 136, 159, 169, 192, 576, 635, 798, 1321
LmnI GCTCC 6 cut(s) 247, 319, 410, 494, 773, 1187
Lsp1109I GCAGC 1 cut(s) 404
LweI GCATC 4 cut(s) 742, 940, 982, 1059
MabI ACCWGGT 1 cut(s) 228
MaeI CTAG 3 cut(s) 788, 876, 1169
MaeII ACGT 7 cut(s) 146, 207, 386, 593, 624, 869, 891
MaeIII GTNAC 4 cut(s) 35, 142, 208, 687
MalI GATC 9 cut(s) 116, 138, 161, 171, 194, 578, 637, 800, 1323
MbiI CCGCTC 1 cut(s) 497
MboI GATC 9 cut(s) 114, 136, 159, 169, 192, 576, 635, 798, 1321
MboII GAAGA 7 cut(s) 313, 773, 864, 1034, 1078, 1157, 1268
MflI RGATCY 4 cut(s) 136, 576, 635, 1321
MhlI GDGCHC 1 cut(s) 113
MluCI AATT 4 cut(s) 810, 862, 902, 938
MlyI GAGTC 2 cut(s) 247, 1313
MmeI TCCRAC 2 cut(s) 102, 1272
MroNI GCCGGC 2 cut(s) 68, 413
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 2 cut(s) 861, 1317
MspA1I CMGCKG 2 cut(s) 539, 734
MspI CCGG 9 cut(s) 30, 69, 106, 156, 201, 337, 344, 414, 1052
MspR9I CCNGG 4 cut(s) 30, 157, 230, 337
MvaI CCWGG 1 cut(s) 230
MvnI CGCG 2 cut(s) 44, 1032
MwoI GCNNNNNNNGC 9 cut(s) 69, 472, 494, 503, 512, 524, 731, 782, 1207
NaeI GCCGGC 2 cut(s) 70, 415
NciI CCSGG 3 cut(s) 30, 157, 337
NcoI CCATGG 1 cut(s) 1308
NdeII GATC 9 cut(s) 114, 136, 159, 169, 192, 576, 635, 798, 1321
NgoMIV GCCGGC 2 cut(s) 68, 413
NlaIII CATG 8 cut(s) 15, 62, 554, 661, 859, 931, 1051, 1312
NlaIV GGNNCC 4 cut(s) 110, 249, 412, 637
NmeAIII GCCGAG 1 cut(s) 979
NmuCI GTSAC 4 cut(s) 35, 142, 208, 687
NspI RCATGY 1 cut(s) 859
PaeR7I CTCGAG 1 cut(s) 376
PagI TCATGA 1 cut(s) 550
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PdiI GCCGGC 2 cut(s) 70, 415
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 5 cut(s) 259, 340, 370, 644, 1273
PflFI GACNNNGTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 1116
PkrI GCNGC 5 cut(s) 43, 74, 419, 499, 517
Ple19I CGATCG 1 cut(s) 172
PleI GAGTC 2 cut(s) 247, 1312
PpsI GAGTC 2 cut(s) 247, 1312
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 4 cut(s) 110, 249, 412, 637
PspPI GGNCC 3 cut(s) 27, 1027, 1332
PstI CTGCAG 1 cut(s) 422
PstNI CAGNNNCTG 1 cut(s) 1201
PsuI RGATCY 4 cut(s) 136, 576, 635, 1321
PsyI GACNNNGTC 1 cut(s) 7
PvuI CGATCG 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 734
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
SaqAI TTAA 2 cut(s) 861, 1317
SatI GCNGC 5 cut(s) 42, 73, 418, 498, 516
Sau3AI GATC 9 cut(s) 114, 136, 159, 169, 192, 576, 635, 798, 1321
Sau96I GGNCC 3 cut(s) 27, 1027, 1332
SchI GAGTC 2 cut(s) 247, 1313
ScrFI CCNGG 4 cut(s) 30, 157, 230, 337
SduI GDGCHC 1 cut(s) 113
SexAI ACCWGGT 1 cut(s) 228
SfaNI GCATC 4 cut(s) 742, 940, 982, 1059
SfcI CTRYAG 1 cut(s) 418
Sfr274I CTCGAG 1 cut(s) 376
SlaI CTCGAG 1 cut(s) 376
SmlI CTYRAG 3 cut(s) 376, 880, 1325
SmoI CTYRAG 3 cut(s) 376, 880, 1325
Sse9I AATT 4 cut(s) 810, 862, 902, 938
SsiI CCGC 9 cut(s) 42, 72, 395, 475, 497, 515, 539, 605, 1030
SspMI CTAG 3 cut(s) 788, 876, 1169
StyD4I CCNGG 4 cut(s) 28, 155, 228, 335
StyI CCWWGG 1 cut(s) 1308
TaaI ACNGT 1 cut(s) 677
TaiI ACGT 7 cut(s) 149, 210, 389, 596, 627, 872, 894
TaqI TCGA 3 cut(s) 195, 377, 990
TaqII GACCGA 1 cut(s) 1268
TasI AATT 4 cut(s) 810, 862, 902, 938
TauI GCSGC 4 cut(s) 44, 75, 500, 518
TfiI GAWTC 5 cut(s) 259, 340, 370, 644, 1273
Tru1I TTAA 2 cut(s) 861, 1317
Tru9I TTAA 2 cut(s) 861, 1317
TseFI GTSAC 4 cut(s) 35, 142, 208, 687
TseI GCWGC 1 cut(s) 417
Tsp45I GTSAC 4 cut(s) 35, 142, 208, 687
TspDTI ATGAA 4 cut(s) 146, 539, 567, 636
TspGWI ACGGA 2 cut(s) 423, 507
Tth111I GACNNNGTC 1 cut(s) 7
Van91I CCANNNNNTGG 1 cut(s) 1116
XapI RAATTY 1 cut(s) 902
XbaI TCTAGA 1 cut(s) 1168
XceI RCATGY 1 cut(s) 859
XhoI CTCGAG 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 152
XmnI GAANNNNTTC 1 cut(s) 308
XspI CTAG 3 cut(s) 788, 876, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.