MD13G1017300.v1.1

U-box domain-containing protein 21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001443
Physical Location & Seq
Forward (+)
1126641 .. 1127972
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1017300.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGACCTTGTCATGGAGAAGGCTGAGAGCCGGCCGCCGTGCTGCCAAGGAGGGTGAGGCCGGCGACATGGAGCTGACCATACCAAACCACTTCCGGTGCCCAATTTCACTCGAGTTGATGAAGGATCCGGTCACGCTGTCCACCGGGATCACGTACGACCGTCAGAGCATAGAGACGTGGATTGAAGCCGGCAACTTCAAGTGCCCGATAACAAACCAAGTCCTCACAAGCTTCGATCCAATTCCTAATCACACCATCCGCAAGATGATACAGGGTTGGTGCGTTGAGAAAAAGTCTTTTGGAATCGAGCGCATTCCCACCCCTCGCATTCCGATCAGTTCGGTTCAGGTGACCGAGATTTTATCAAAGATTACGACATCGAGTCATCATCAGAACAAGGATGATTGCCAAGTTTTGGTGGCGAAGATCAATGCGTTGGCGAAAGAAAGCGAGCGTAACAAGCGTTGCATTGTGGCTAGTGGCACGGGGAGCGTTCTAGCGGGTGCGTTTAATGCATTTTCAAGTTCTCAACACCATCAAAACGTTGCCGTTTTGGAGGAGATTTTGTCTGCTTTGACTTTGGTTTTCCCGCTTGACAAGGAGGCTAACTTGTACCTCGGATCGCCTGCTTCGTTGTGTTGCATGGTGTGGTTTTTGGAGAGTGGAGATTTGTCACAAAGACGAAACGCATCGTTGGTGCTCAAAGAGACCGCTGTCTCATCTGATCATGATCATCAGAAGACCGATGCTTTGGCAGAGATCGAAGGAGCTCTGGAGGCGCTAGTGAAGCTGATCAAAGAACCAGTTTGCCCTACTTCTACAAAAGCTTCCTTGGTCATCATGTATCGAATGGTCAACTCATCATCCTCCTCTCTTTCGAAAGAGCAAATCAAAGAGAGATTTGTAGAGATGGGATTGGTGTCGCTGCTTTCGGACATTCTTGTAGATGCCGAAAGAAGCATTTGCGAGAAGGCGTTGGGAGTGCTAGACGGACTTTGCGGAAGCAAGCAAGGCAGGGAAAAGGCCTACCTTCATGCCTTGACCATGCCGGTTGTGGTGAAGAAGATACTTAGGGTTTCGGATTTGGCCACGGAGTTTTCCGTGTCTATTCTTTGGAAGCTTTGCAAGAACGAGACTAGGGAAGACGGAGGTGTGCTCGTGGAGGCCCTTCAAGTGGGTGCGTTTCAGAAGCTCTTGCTACTTTTGCAAGTTGGTTGTGCGGAGAGGACCAAAGACAAGGCCACGGACTTGTTAAAGGCATTGAACATTCATAGGGAGAGGTTGGAGTGTATTGATTCTATGGATTTTAAGCAGCTTAAGAGGCCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

444

Amino Acids

49.1

Weight (kDa)

8.16

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 27 - 98 2.8e-21 U-box domain
ARM_PUB PF25598 117 - 438 9.5e-69 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 96
AccB7I CCANNNNNTGG 1 cut(s) 415
AciI CCGC 7 cut(s) 34, 259, 500, 590, 711, 999, 1220
AclI AACGTT 1 cut(s) 543
AclWI GGATC 5 cut(s) 119, 132, 155, 230, 628
AcoI YGGCCR 2 cut(s) 31, 1086
AfaI GTAC 2 cut(s) 155, 614
AfiI CCNNNNNNNGG 3 cut(s) 12, 415, 1174
AflII CTTAAG 1 cut(s) 1316
AgsI TTSAA 5 cut(s) 185, 199, 522, 1172, 1264
AhdI GACNNNNNGTC 1 cut(s) 381
AjiI CACGTC 1 cut(s) 177
AluBI AGCT 8 cut(s) 73, 231, 770, 790, 827, 1120, 1192, 1315
AluI AGCT 8 cut(s) 73, 231, 770, 790, 827, 1120, 1192, 1315
Alw21I GWGCWC 3 cut(s) 702, 772, 1158
Alw26I GTCTC 4 cut(s) 167, 701, 721, 1127
AlwI GGATC 5 cut(s) 119, 132, 155, 230, 628
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 7 cut(s) 31, 57, 1023, 1086, 1164, 1239, 1322
ApeKI GCWGC 3 cut(s) 41, 925, 1312
ArsI GACNNNNNNTTYG 2 cut(s) 733, 765
AspLEI GCGC 2 cut(s) 312, 781
AspS9I GGNCC 3 cut(s) 1165, 1227, 1323
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 3 cut(s) 65, 361, 1069
AsuII TTCGAA 1 cut(s) 878
AvaI CYCGRG 1 cut(s) 110
AvaII GGWCC 1 cut(s) 1227
BaeGI GKGCMC 2 cut(s) 101, 206
BalI TGGCCA 1 cut(s) 1088
BamHI GGATCC 1 cut(s) 124
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 772
BauI CACGAG 1 cut(s) 1157
BbsI GAAGAC 2 cut(s) 746, 1149
Bbv12I GWGCWC 3 cut(s) 702, 772, 1158
BbvI GCAGC 3 cut(s) 28, 912, 1324
BccI CCATC 3 cut(s) 263, 543, 904
BceAI ACGGC 2 cut(s) 21, 533
BclI TGATCA 3 cut(s) 724, 730, 792
BcnI CCSGG 1 cut(s) 145
BcoDI GTCTC 4 cut(s) 167, 701, 721, 1127
BfaI CTAG 5 cut(s) 477, 497, 782, 986, 1137
BfoI RGCGCY 1 cut(s) 782
BfrI CTTAAG 1 cut(s) 1316
BisI GCNGC 4 cut(s) 34, 42, 926, 1313
BlsI GCNGC 4 cut(s) 35, 43, 927, 1314
Bme1390I CCNGG 1 cut(s) 145
Bme18I GGWCC 1 cut(s) 1227
BmeRI GACNNNNNGTC 1 cut(s) 381
BmeT110I CYCGRG 1 cut(s) 110
BmgBI CACGTC 1 cut(s) 177
BmgT120I GGNCC 3 cut(s) 1165, 1227, 1323
BmiI GGNNCC 2 cut(s) 98, 126
BmrFI CCNGG 1 cut(s) 145
BmsI GCATC 3 cut(s) 698, 736, 937
BoxI GACNNNNGTC 1 cut(s) 713
BpiI GAAGAC 2 cut(s) 746, 1149
BplI GAGNNNNNCTC 2 cut(s) 1140, 1172
BpmI CTGGAG 1 cut(s) 794
Bpu14I TTCGAA 1 cut(s) 878
BpuMI CCSGG 1 cut(s) 145
BsaAI YACGTR 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 729
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 5 cut(s) 45, 616, 831, 1089, 1242
BsaWI WCCGGW 2 cut(s) 93, 127
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 415, 1174
Bse118I RCCGGY 4 cut(s) 29, 59, 188, 1048
Bse1I ACTGG 1 cut(s) 803
Bse8I GATNNNNATC 1 cut(s) 729
BseDI CCNNGG 5 cut(s) 45, 616, 831, 1089, 1242
BseGI GGATG 3 cut(s) 255, 406, 863
BseJI GATNNNNATC 1 cut(s) 729
BseLI CCNNNNNNNGG 3 cut(s) 12, 415, 1174
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 1 cut(s) 803
BseRI GAGGAG 2 cut(s) 572, 859
BseSI GKGCMC 2 cut(s) 101, 206
BseX3I CGGCCG 1 cut(s) 31
BseXI GCAGC 3 cut(s) 28, 912, 1324
Bsh1285I CGRYCG 2 cut(s) 34, 160
BshFI GGCC 7 cut(s) 33, 59, 1025, 1088, 1166, 1241, 1324
BshNI GGYRCC 1 cut(s) 96
BsiEI CGRYCG 2 cut(s) 34, 160
BsiHKAI GWGCWC 3 cut(s) 702, 772, 1158
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 7 cut(s) 30, 60, 94, 128, 144, 189, 1049
BsiWI CGTACG 1 cut(s) 153
BslI CCNNNNNNNGG 3 cut(s) 12, 415, 1174
BsmAI GTCTC 4 cut(s) 167, 701, 721, 1127
BsmBI CGTCTC 1 cut(s) 167
BsmI GAATGC 2 cut(s) 312, 327
BsnI GGCC 7 cut(s) 33, 59, 1025, 1088, 1166, 1241, 1324
Bso31I GGTCTC 1 cut(s) 701
BsoBI CYCGRG 1 cut(s) 110
Bsp119I TTCGAA 1 cut(s) 878
Bsp1286I GDGCHC 5 cut(s) 101, 206, 702, 772, 1158
BspACI CCGC 7 cut(s) 34, 259, 500, 590, 711, 999, 1220
BspANI GGCC 7 cut(s) 33, 59, 1025, 1088, 1166, 1241, 1324
BspCNI CTCAG 1 cut(s) 15
BspHI TCATGA 1 cut(s) 727
BspLI GGNNCC 2 cut(s) 98, 126
BspPI GGATC 5 cut(s) 119, 132, 155, 230, 628
BspT104I TTCGAA 1 cut(s) 878
BspT107I GGYRCC 1 cut(s) 96
BspTI CTTAAG 1 cut(s) 1316
BspTNI GGTCTC 1 cut(s) 701
BsrFI RCCGGY 4 cut(s) 29, 59, 188, 1048
BsrI ACTGG 1 cut(s) 803
BssAI RCCGGY 4 cut(s) 29, 59, 188, 1048
BssECI CCNNGG 5 cut(s) 45, 616, 831, 1089, 1242
BssSI CACGAG 1 cut(s) 1157
BssT1I CCWWGG 2 cut(s) 45, 831
Bst2BI CACGAG 1 cut(s) 1157
Bst4CI ACNGT 1 cut(s) 161
BstAFI CTTAAG 1 cut(s) 1316
BstBAI YACGTR 1 cut(s) 153
BstBI TTCGAA 1 cut(s) 878
BstC8I GCNNGC 6 cut(s) 31, 61, 190, 452, 627, 1007
BstDEI CTNAG 2 cut(s) 23, 1070
BstDSI CCRYGG 2 cut(s) 1089, 1242
BstEII GGTNACC 1 cut(s) 349
BstF5I GGATG 3 cut(s) 255, 406, 863
BstH2I RGCGCY 1 cut(s) 782
BstHHI GCGC 2 cut(s) 312, 781
BstMAI GTCTC 4 cut(s) 167, 701, 721, 1127
BstMCI CGRYCG 2 cut(s) 34, 160
BstMWI GCNNNNNNNGC 8 cut(s) 460, 489, 512, 776, 787, 1011, 1204, 1321
BstPAI GACNNNNGTC 1 cut(s) 713
BstPI GGTNACC 1 cut(s) 349
BstSCI CCNGG 1 cut(s) 143
BstSLI GKGCMC 2 cut(s) 101, 206
BstV1I GCAGC 3 cut(s) 28, 912, 1324
BstV2I GAAGAC 2 cut(s) 746, 1149
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BstZI CGGCCG 1 cut(s) 31
BsuRI GGCC 7 cut(s) 33, 59, 1025, 1088, 1166, 1241, 1324
BtgI CCRYGG 2 cut(s) 1089, 1242
BtrI CACGTC 1 cut(s) 177
BtsCI GGATG 3 cut(s) 255, 406, 863
Cac8I GCNNGC 6 cut(s) 31, 61, 190, 452, 627, 1007
CciI TCATGA 1 cut(s) 727
CfoI GCGC 2 cut(s) 312, 781
Cfr10I RCCGGY 4 cut(s) 29, 59, 188, 1048
Cfr13I GGNCC 3 cut(s) 1165, 1227, 1323
Csp6I GTAC 2 cut(s) 154, 613
CviAII CATG 7 cut(s) 12, 67, 643, 728, 841, 1034, 1045
CviQI GTAC 2 cut(s) 154, 613
DdeI CTNAG 2 cut(s) 23, 1070
DriI GACNNNNNGTC 1 cut(s) 381
EaeI YGGCCR 2 cut(s) 31, 1086
EagI CGGCCG 1 cut(s) 31
Eam1105I GACNNNNNGTC 1 cut(s) 381
Ecl136II GAGCTC 1 cut(s) 770
EclXI CGGCCG 1 cut(s) 31
Eco130I CCWWGG 2 cut(s) 45, 831
Eco147I AGGCCT 1 cut(s) 1025
Eco24I GRGCYC 1 cut(s) 772
Eco31I GGTCTC 1 cut(s) 701
Eco47I GGWCC 1 cut(s) 1227
Eco52I CGGCCG 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 770
Eco88I CYCGRG 1 cut(s) 110
Eco91I GGTNACC 1 cut(s) 349
EcoICRI GAGCTC 1 cut(s) 770
EcoO109I RGGNCCY 2 cut(s) 1165, 1323
EcoO65I GGTNACC 1 cut(s) 349
EcoT14I CCWWGG 2 cut(s) 45, 831
EcoT22I ATGCAT 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 772
ErhI CCWWGG 2 cut(s) 45, 831
Esp3I CGTCTC 1 cut(s) 167
FaeI CATG 7 cut(s) 15, 70, 646, 731, 844, 1037, 1048
FalI AAGNNNNNCTT 1 cut(s) 1310
FatI CATG 7 cut(s) 11, 66, 642, 727, 840, 1033, 1044
FauI CCCGC 2 cut(s) 493, 597
FbaI TGATCA 3 cut(s) 724, 730, 792
Fnu4HI GCNGC 4 cut(s) 34, 42, 926, 1313
FokI GGATG 3 cut(s) 242, 413, 850
FriOI GRGCYC 1 cut(s) 772
Fsp4HI GCNGC 4 cut(s) 34, 42, 926, 1313
FspBI CTAG 5 cut(s) 477, 497, 782, 986, 1137
GlaI GCGC 2 cut(s) 311, 780
GluI GCNGC 4 cut(s) 34, 42, 926, 1313
GsuI CTGGAG 1 cut(s) 794
HaeII RGCGCY 1 cut(s) 782
HaeIII GGCC 7 cut(s) 33, 59, 1025, 1088, 1166, 1241, 1324
HapII CCGG 7 cut(s) 30, 60, 94, 128, 144, 189, 1049
HhaI GCGC 2 cut(s) 312, 781
Hin1II CATG 7 cut(s) 15, 70, 646, 731, 844, 1037, 1048
Hin6I GCGC 2 cut(s) 310, 779
HinP1I GCGC 2 cut(s) 310, 779
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 3 cut(s) 229, 825, 1118
HinfI GANTC 3 cut(s) 303, 382, 1295
HpaII CCGG 7 cut(s) 30, 60, 94, 128, 144, 189, 1049
HphI GGTGA 3 cut(s) 65, 361, 1069
Hpy166II GTNNAC 2 cut(s) 141, 856
Hpy188I TCNGA 9 cut(s) 165, 333, 393, 620, 724, 738, 934, 1081, 1188
Hpy188III TCNNGA 2 cut(s) 728, 773
Hpy8I GTNNAC 2 cut(s) 141, 856
HpyAV CCTTC 6 cut(s) 12, 115, 758, 964, 1040, 1178
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4IV ACGT 3 cut(s) 152, 176, 543
HpyCH4V TGCA 5 cut(s) 468, 515, 642, 1125, 1207
HpyF10VI GCNNNNNNNGC 8 cut(s) 460, 489, 512, 776, 787, 1011, 1204, 1321
HpyF3I CTNAG 2 cut(s) 23, 1070
HpySE526I ACGT 3 cut(s) 152, 176, 543
Hsp92II CATG 7 cut(s) 15, 70, 646, 731, 844, 1037, 1048
HspAI GCGC 2 cut(s) 310, 779
KroI GCCGGC 3 cut(s) 29, 59, 188
KroNI GCCGGC 3 cut(s) 31, 61, 190
Ksp22I TGATCA 3 cut(s) 724, 730, 792
LmnI GCTCC 3 cut(s) 70, 489, 767
Lsp1109I GCAGC 3 cut(s) 28, 912, 1324
LweI GCATC 3 cut(s) 698, 736, 937
MaeI CTAG 5 cut(s) 477, 497, 782, 986, 1137
MaeII ACGT 3 cut(s) 152, 176, 543
MaeIII GTNAC 4 cut(s) 130, 349, 455, 672
MboII GAAGA 5 cut(s) 436, 751, 1072, 1075, 1154
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 5 cut(s) 101, 206, 702, 772, 1158
MlsI TGGCCA 1 cut(s) 1088
MluCI AATT 2 cut(s) 102, 240
MluNI TGGCCA 1 cut(s) 1088
MlyI GAGTC 1 cut(s) 391
MmeI TCCRAC 1 cut(s) 1263
Mox20I TGGCCA 1 cut(s) 1088
Mph1103I ATGCAT 1 cut(s) 517
MroNI GCCGGC 3 cut(s) 29, 59, 188
MscI TGGCCA 1 cut(s) 1088
MseI TTAA 4 cut(s) 510, 1253, 1308, 1317
Msp20I TGGCCA 1 cut(s) 1088
MspA1I CMGCKG 1 cut(s) 713
MspCI CTTAAG 1 cut(s) 1316
MspI CCGG 7 cut(s) 30, 60, 94, 128, 144, 189, 1049
MspR9I CCNGG 1 cut(s) 145
Mva1269I GAATGC 2 cut(s) 312, 327
MwoI GCNNNNNNNGC 8 cut(s) 460, 489, 512, 776, 787, 1011, 1204, 1321
NaeI GCCGGC 3 cut(s) 31, 61, 190
NciI CCSGG 1 cut(s) 145
NgoMIV GCCGGC 3 cut(s) 29, 59, 188
NlaIII CATG 7 cut(s) 15, 70, 646, 731, 844, 1037, 1048
NlaIV GGNNCC 2 cut(s) 98, 126
NmuCI GTSAC 3 cut(s) 130, 349, 672
NsiI ATGCAT 1 cut(s) 517
NspV TTCGAA 1 cut(s) 878
PaeR7I CTCGAG 1 cut(s) 110
PagI TCATGA 1 cut(s) 727
PceI AGGCCT 1 cut(s) 1025
PcsI WCGNNNNNNNCGW 1 cut(s) 629
PctI GAATGC 2 cut(s) 312, 327
PdiI GCCGGC 3 cut(s) 31, 61, 190
PfeI GAWTC 2 cut(s) 303, 1295
Pfl23II CGTACG 1 cut(s) 153
PflFI GACNNNGTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 415
PkrI GCNGC 4 cut(s) 35, 43, 927, 1314
PleI GAGTC 1 cut(s) 390
PpsI GAGTC 1 cut(s) 390
Ppu21I YACGTR 1 cut(s) 153
PshAI GACNNNNGTC 1 cut(s) 713
Psp124BI GAGCTC 1 cut(s) 772
Psp1406I AACGTT 1 cut(s) 543
PspEI GGTNACC 1 cut(s) 349
PspLI CGTACG 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 98, 126
PspPI GGNCC 3 cut(s) 1165, 1227, 1323
PspXI VCTCGAGB 1 cut(s) 110
PsuI RGATCY 1 cut(s) 124
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 2 cut(s) 155, 614
RsaNI GTAC 2 cut(s) 154, 613
SacI GAGCTC 1 cut(s) 772
SaqAI TTAA 4 cut(s) 510, 1253, 1308, 1317
SatI GCNGC 4 cut(s) 34, 42, 926, 1313
Sau96I GGNCC 3 cut(s) 1165, 1227, 1323
SchI GAGTC 1 cut(s) 391
ScrFI CCNGG 1 cut(s) 145
SduI GDGCHC 5 cut(s) 101, 206, 702, 772, 1158
SfaNI GCATC 3 cut(s) 698, 736, 937
Sfr274I CTCGAG 1 cut(s) 110
SfuI TTCGAA 1 cut(s) 878
SinI GGWCC 1 cut(s) 1227
SlaI CTCGAG 1 cut(s) 110
SmlI CTYRAG 2 cut(s) 110, 1316
SmoI CTYRAG 2 cut(s) 110, 1316
Sse9I AATT 2 cut(s) 102, 240
SseBI AGGCCT 1 cut(s) 1025
SsiI CCGC 7 cut(s) 34, 259, 500, 590, 711, 999, 1220
SspMI CTAG 5 cut(s) 477, 497, 782, 986, 1137
SstI GAGCTC 1 cut(s) 772
StuI AGGCCT 1 cut(s) 1025
StyD4I CCNGG 1 cut(s) 143
StyI CCWWGG 2 cut(s) 45, 831
TaaI ACNGT 1 cut(s) 161
TaiI ACGT 3 cut(s) 155, 179, 546
TaqI TCGA 7 cut(s) 111, 234, 306, 380, 762, 847, 878
TaqII GACCGA 2 cut(s) 368, 758
TasI AATT 2 cut(s) 102, 240
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 2 cut(s) 303, 1295
Tru1I TTAA 4 cut(s) 510, 1253, 1308, 1317
Tru9I TTAA 4 cut(s) 510, 1253, 1308, 1317
TseFI GTSAC 3 cut(s) 130, 349, 672
TseI GCWGC 3 cut(s) 41, 925, 1312
Tsp45I GTSAC 3 cut(s) 130, 349, 672
TspDTI ATGAA 3 cut(s) 134, 1022, 1259
TspGWI ACGGA 5 cut(s) 1005, 1090, 1106, 1161, 1259
Tth111I GACNNNGTC 1 cut(s) 7
Van91I CCANNNNNTGG 1 cut(s) 415
Vha464I CTTAAG 1 cut(s) 1316
VpaK11BI GGWCC 1 cut(s) 1227
XcmI CCANNNNNNNNNTGG 1 cut(s) 1051
XhoI CTCGAG 1 cut(s) 110
XspI CTAG 5 cut(s) 477, 497, 782, 986, 1137
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.