pycom17g26260

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001443
Physical Location & Seq
Forward (+)
24588095 .. 24589929
1835 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g26260.1

Sequence Viewer

Length: 1476 bp
ATGCACTGTTATGTAGCAAAGTCTCATCGAGCTCCCCACATTTCTCACAACCAAAAACAACACGAATACTCAAAGCAAATTCTCTCATCGATTTCTAATTTATTTCTTTTGTACTTTGATTCAAAACAACGAACAATGATCTTATCTTGGGGAAGAAGAAGGGCCGGACGTAAAGCCAACAACAAGAAACAATTTTGTAATTTGGACATGGACGTGGAGGAGATCGCCATTCCGACGCATTTCCGGTGCCCGATTTCCCTCGACTTGATGAAAGATCCGGTGACGCTTTCGACGGGAATGACGTACGACAGGTCGAGCATTGAGATGTGGATCGAGACCGGCAACAGTACGTGTCCGGTGACTAATCAGGAACTAGTGAGTTTTGACCAGATTCCGAACCATGCACTGCGTCGGATGATCCAGGATTGGTGTGTGGAGAACAGGTCTAATGGCATCGAGCGGATTCCAACGCCGCGAATCCCCGTGACTCCCTATGAGATTTTGAGGATCCGCGAGAGAGTTGCGGGGGCAGCGCGGCGTTTGGATGAGAAGAAGTGTCATGAACTGGTGGGGAAGATTAAAGCGTGGGGCAAGGAAAGCGAGCGGAACAAGAAATGTATGGTCGAGAACGGAATTGGCAGCTGCTTATCCGCTGTGTTCGAGTCCTTTGCCGACGGTTCAATGGACAAGCATGTTGAGATATTGGTGGAAATTCTATCAGTGCTGCCGTGGATTTTTCCATTGAGTGAAGAAGGCCAATCAAAGCTTGGATCTTCAGGGTGTTTGCGATCTTTTGTGTCGTTTTTGGAGGGGAAAGATCTAACAGCACGGAAAAACGCGGTTTTGGTACTTAGAAAGTTGTTTTCTTCGGATCAAAAATACGTGGATGCTTTCTGGGCGATTGAAGGAGGTACTGAAGCAATTGTTGGGATCATTAGAGAACCTATTTGTCCTCAAGCGACCAAGGCCGCTCTAGCTTGTGTTCTTTTCATGAGTTCATCACCGCCATCCGCGCAAACCAGTGACGAAATCAGATCAAGATTTGTGGAATTGGGTTTGGTTTCTTTGCTGCTGGAAATTCTTGTTGAGGCAGAGAAAGGAGTTTCGGAGAGAGCTTTGGGTGTTTTGGAGGTTCTTTGCGACTGCAAACAAGGGAGGGAAAAGGCCCTTGAGAATGCTCTCACAATGCCTCTTTTGGTCAAGAAGATTTTGAGGATTTCAGAGTTGGGAACGGAGTTTTCGGTGTCTATAATCTGGAAGCTGTGCAGGATTGAAACCGAGGAAAGCGTTTTGATTGAGGCGCTTCAAGTGGGTGCTTTTCAGAAGCTGTTGGTGGTCTTGCAGGTCGGGGTTAGTGATGGGATCAAGGACAAGGTTACTGACTTGTTGAAATTGTTTAATCTGCACACGAGTAAATTGGACTGTGTTGATTCCTCCATGGATTTCAAGTACCTGAAGAGTTCAAAAATGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

492

Amino Acids

55.21

Weight (kDa)

8.26

Isoelectric Point (pI)

51.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 77 - 148 1.6e-20 U-box domain
ARM_PUB PF25598 166 - 484 3.7e-70 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1333
AccB1I GGYRCC 1 cut(s) 246
AccBSI CCGCTC 3 cut(s) 460, 604, 971
AccII CGCG 5 cut(s) 475, 513, 535, 839, 1013
AclWI GGATC 9 cut(s) 269, 338, 412, 502, 515, 778, 879, 938, 1370
AcsI RAATTY 3 cut(s) 78, 711, 1077
AcuI CTGAAG 3 cut(s) 759, 936, 1475
AfaI GTAC 6 cut(s) 113, 305, 349, 849, 913, 1451
AflIII ACRYGT 1 cut(s) 350
AgsI TTSAA 8 cut(s) 123, 681, 905, 1274, 1307, 1390, 1447, 1464
AhlI ACTAGT 1 cut(s) 373
AjiI CACGTC 1 cut(s) 214
AjnI CCWGG 1 cut(s) 420
AjuI GAANNNNNNNTTGG 2 cut(s) 909, 941
AluBI AGCT 7 cut(s) 32, 642, 766, 977, 1115, 1261, 1327
AluI AGCT 7 cut(s) 32, 642, 766, 977, 1115, 1261, 1327
Alw21I GWGCWC 1 cut(s) 34
Alw26I GTCTC 2 cut(s) 27, 329
AlwI GGATC 9 cut(s) 269, 338, 412, 502, 515, 778, 879, 938, 1370
AlwNI CAGNNNCTG 1 cut(s) 1327
AoxI GGCC 4 cut(s) 162, 754, 966, 1164
ApeKI GCWGC 5 cut(s) 530, 639, 642, 724, 1069
ApoI RAATTY 3 cut(s) 78, 711, 1077
AspLEI GCGC 3 cut(s) 535, 1015, 1303
AspS9I GGNCC 2 cut(s) 162, 1165
AsuHPI GGTGA 3 cut(s) 292, 370, 993
BaeGI GKGCMC 1 cut(s) 251
BamHI GGATCC 1 cut(s) 507
BanI GGYRCC 1 cut(s) 246
BanII GRGCYC 1 cut(s) 34
BauI CACGAG 1 cut(s) 1408
Bbv12I GWGCWC 1 cut(s) 34
BbvI GCAGC 5 cut(s) 542, 629, 651, 711, 1056
BccI CCATC 2 cut(s) 1015, 1352
BceAI ACGGC 1 cut(s) 712
BcgI CGANNNNNNTGC 4 cut(s) 503, 537, 650, 684
BciT130I CCWGG 1 cut(s) 422
BcoDI GTCTC 2 cut(s) 27, 329
BcuI ACTAGT 1 cut(s) 373
BfaI CTAG 2 cut(s) 374, 974
BfoI RGCGCY 1 cut(s) 1304
BfuAI ACCTGC 1 cut(s) 1333
BglII AGATCT 1 cut(s) 817
BisI GCNGC 8 cut(s) 473, 531, 536, 640, 643, 725, 969, 1070
BlsI GCNGC 8 cut(s) 474, 532, 537, 641, 644, 726, 970, 1071
Bme1390I CCNGG 1 cut(s) 422
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 2 cut(s) 162, 1165
BmiI GGNNCC 2 cut(s) 248, 509
BmrFI CCNGG 1 cut(s) 422
BmsI GCATC 2 cut(s) 462, 877
BpuEI CTTGAG 2 cut(s) 939, 1190
Bsa29I ATCGAT 1 cut(s) 89
BsaAI YACGTR 2 cut(s) 351, 883
BsaBI GATNNNNATC 1 cut(s) 329
BsaI GGTCTC 1 cut(s) 329
BsaJI CCNNGG 4 cut(s) 728, 963, 1278, 1437
BsaWI WCCGGW 3 cut(s) 243, 277, 355
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 2 cut(s) 570, 1020
Bse8I GATNNNNATC 1 cut(s) 329
BseBI CCWGG 1 cut(s) 422
BseCI ATCGAT 1 cut(s) 89
BseDI CCNNGG 4 cut(s) 728, 963, 1278, 1437
BseGI GGATG 4 cut(s) 420, 550, 892, 1007
BseJI GATNNNNATC 1 cut(s) 329
BseNI ACTGG 2 cut(s) 570, 1020
BseRI GAGGAG 1 cut(s) 233
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 5 cut(s) 542, 629, 651, 711, 1056
BsgI GTGCAG 2 cut(s) 1285, 1388
Bsh1236I CGCG 5 cut(s) 475, 513, 535, 839, 1013
BshFI GGCC 4 cut(s) 164, 756, 968, 1166
BshNI GGYRCC 1 cut(s) 246
BshVI ATCGAT 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 34
BsiSI CCGG 5 cut(s) 165, 244, 278, 339, 356
BsiWI CGTACG 1 cut(s) 303
BsmAI GTCTC 2 cut(s) 27, 329
BsmI GAATGC 1 cut(s) 1180
BsnI GGCC 4 cut(s) 164, 756, 968, 1166
Bso31I GGTCTC 1 cut(s) 329
Bsp1286I GDGCHC 2 cut(s) 34, 251
Bsp19I CCATGG 1 cut(s) 1437
BspANI GGCC 4 cut(s) 164, 756, 968, 1166
BspDI ATCGAT 1 cut(s) 89
BspFNI CGCG 5 cut(s) 475, 513, 535, 839, 1013
BspHI TCATGA 2 cut(s) 559, 990
BspLI GGNNCC 2 cut(s) 248, 509
BspMI ACCTGC 1 cut(s) 1333
BspPI GGATC 9 cut(s) 269, 338, 412, 502, 515, 778, 879, 938, 1370
BspT107I GGYRCC 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 329
BsrBI CCGCTC 3 cut(s) 460, 604, 971
BsrFI RCCGGY 1 cut(s) 338
BsrI ACTGG 2 cut(s) 570, 1020
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 4 cut(s) 728, 963, 1278, 1437
BssSI CACGAG 1 cut(s) 1408
BssT1I CCWWGG 2 cut(s) 963, 1437
Bst2BI CACGAG 1 cut(s) 1408
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 4 cut(s) 8, 347, 677, 1424
Bst6I CTCTTC 1 cut(s) 1451
BstBAI YACGTR 2 cut(s) 351, 883
BstC8I GCNNGC 1 cut(s) 602
BstDEI CTNAG 1 cut(s) 851
BstDSI CCRYGG 2 cut(s) 728, 1437
BstF5I GGATG 4 cut(s) 420, 550, 892, 1007
BstFNI CGCG 5 cut(s) 475, 513, 535, 839, 1013
BstH2I RGCGCY 1 cut(s) 1304
BstHHI GCGC 3 cut(s) 535, 1015, 1303
BstMAI GTCTC 2 cut(s) 27, 329
BstMWI GCNNNNNNNGC 6 cut(s) 530, 597, 896, 965, 974, 1012
BstNI CCWGG 1 cut(s) 422
BstNSI RCATGY 1 cut(s) 695
BstSCI CCNGG 1 cut(s) 420
BstSLI GKGCMC 1 cut(s) 251
BstUI CGCG 5 cut(s) 475, 513, 535, 839, 1013
BstV1I GCAGC 5 cut(s) 542, 629, 651, 711, 1056
BstX2I RGATCY 4 cut(s) 274, 507, 770, 817
BstYI RGATCY 4 cut(s) 274, 507, 770, 817
Bsu15I ATCGAT 1 cut(s) 89
BsuRI GGCC 4 cut(s) 164, 756, 968, 1166
BsuTUI ATCGAT 1 cut(s) 89
BtgI CCRYGG 2 cut(s) 728, 1437
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 4 cut(s) 420, 550, 892, 1007
BtsI GCAGTG 1 cut(s) 404
BtsIMutI CAGTG 4 cut(s) 4, 404, 726, 1027
BveI ACCTGC 1 cut(s) 1333
Cac8I GCNNGC 1 cut(s) 602
CaiI CAGNNNCTG 1 cut(s) 1327
CciI TCATGA 2 cut(s) 559, 990
CfoI GCGC 3 cut(s) 535, 1015, 1303
Cfr10I RCCGGY 1 cut(s) 338
Cfr13I GGNCC 2 cut(s) 162, 1165
ClaI ATCGAT 1 cut(s) 89
CseI GACGC 3 cut(s) 244, 292, 398
Csp6I GTAC 6 cut(s) 112, 304, 348, 848, 912, 1450
CspCI CAANNNNNGTGG 2 cut(s) 1026, 1061
CviAII CATG 6 cut(s) 208, 401, 560, 692, 991, 1438
CviQI GTAC 6 cut(s) 112, 304, 348, 848, 912, 1450
DdeI CTNAG 1 cut(s) 851
Eam1104I CTCTTC 1 cut(s) 1451
EarI CTCTTC 1 cut(s) 1451
Ecl136II GAGCTC 1 cut(s) 32
Eco130I CCWWGG 2 cut(s) 963, 1437
Eco24I GRGCYC 1 cut(s) 34
Eco31I GGTCTC 1 cut(s) 329
Eco53kI GAGCTC 1 cut(s) 32
Eco57I CTGAAG 3 cut(s) 759, 936, 1475
EcoICRI GAGCTC 1 cut(s) 32
EcoO109I RGGNCCY 1 cut(s) 1165
EcoRII CCWGG 1 cut(s) 420
EcoT14I CCWWGG 2 cut(s) 963, 1437
EcoT38I GRGCYC 1 cut(s) 34
ErhI CCWWGG 2 cut(s) 963, 1437
FaeI CATG 6 cut(s) 211, 404, 563, 695, 994, 1441
FatI CATG 6 cut(s) 207, 400, 559, 691, 990, 1437
FauI CCCGC 1 cut(s) 517
Fnu4HI GCNGC 8 cut(s) 473, 531, 536, 640, 643, 725, 969, 1070
FokI GGATG 4 cut(s) 427, 557, 899, 994
FriOI GRGCYC 1 cut(s) 34
Fsp4HI GCNGC 8 cut(s) 473, 531, 536, 640, 643, 725, 969, 1070
FspBI CTAG 2 cut(s) 374, 974
GlaI GCGC 3 cut(s) 534, 1014, 1302
GluI GCNGC 8 cut(s) 473, 531, 536, 640, 643, 725, 969, 1070
HaeII RGCGCY 1 cut(s) 1304
HaeIII GGCC 4 cut(s) 164, 756, 968, 1166
HapII CCGG 5 cut(s) 165, 244, 278, 339, 356
HgaI GACGC 3 cut(s) 244, 292, 398
HhaI GCGC 3 cut(s) 535, 1015, 1303
Hin1II CATG 6 cut(s) 211, 404, 563, 695, 994, 1441
Hin6I GCGC 3 cut(s) 533, 1013, 1301
HinP1I GCGC 3 cut(s) 533, 1013, 1301
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 7 cut(s) 119, 391, 463, 477, 487, 662, 1430
HpaII CCGG 5 cut(s) 165, 244, 278, 339, 356
HphI GGTGA 3 cut(s) 292, 370, 993
Hpy188I TCNGA 8 cut(s) 234, 396, 414, 871, 1034, 1108, 1222, 1323
Hpy188III TCNNGA 8 cut(s) 334, 368, 560, 625, 991, 1038, 1201, 1255
Hpy99I CGWCG 4 cut(s) 238, 295, 414, 677
HpyAV CCTTC 3 cut(s) 153, 746, 899
HpyCH4III ACNGT 4 cut(s) 8, 347, 677, 1424
HpyCH4IV ACGT 5 cut(s) 169, 213, 302, 350, 882
HpyCH4V TGCA 6 cut(s) 4, 404, 1146, 1266, 1342, 1405
HpyF10VI GCNNNNNNNGC 6 cut(s) 530, 597, 896, 965, 974, 1012
HpyF3I CTNAG 1 cut(s) 851
HpySE526I ACGT 5 cut(s) 169, 213, 302, 350, 882
Hsp92II CATG 6 cut(s) 211, 404, 563, 695, 994, 1441
HspAI GCGC 3 cut(s) 533, 1013, 1301
LmnI GCTCC 1 cut(s) 37
Lsp1109I GCAGC 5 cut(s) 542, 629, 651, 711, 1056
LweI GCATC 2 cut(s) 462, 877
MaeI CTAG 2 cut(s) 374, 974
MaeII ACGT 5 cut(s) 169, 213, 302, 350, 882
MaeIII GTNAC 5 cut(s) 280, 358, 484, 1022, 1375
MbiI CCGCTC 3 cut(s) 460, 604, 971
MboII GAAGA 9 cut(s) 165, 168, 562, 586, 761, 765, 858, 1216, 1468
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 4 cut(s) 274, 507, 770, 817
MhlI GDGCHC 2 cut(s) 34, 251
MlyI GAGTC 2 cut(s) 481, 671
MmeI TCCRAC 3 cut(s) 257, 392, 491
MseI TTAA 3 cut(s) 579, 1398, 1474
MslI CAYNNNNRTG 3 cut(s) 9, 212, 323
MspA1I CMGCKG 2 cut(s) 642, 653
MspI CCGG 5 cut(s) 165, 244, 278, 339, 356
MspR9I CCNGG 1 cut(s) 422
MunI CAATTG 1 cut(s) 921
Mva1269I GAATGC 1 cut(s) 1180
MvaI CCWGG 1 cut(s) 422
MvnI CGCG 5 cut(s) 475, 513, 535, 839, 1013
MwoI GCNNNNNNNGC 6 cut(s) 530, 597, 896, 965, 974, 1012
NcoI CCATGG 1 cut(s) 1437
NlaIII CATG 6 cut(s) 211, 404, 563, 695, 994, 1441
NlaIV GGNNCC 2 cut(s) 248, 509
NmuCI GTSAC 4 cut(s) 280, 358, 484, 1022
NspI RCATGY 1 cut(s) 695
PagI TCATGA 2 cut(s) 559, 990
PcsI WCGNNNNNNNCGW 1 cut(s) 299
PctI GAATGC 1 cut(s) 1180
PfeI GAWTC 5 cut(s) 119, 391, 463, 477, 1430
Pfl23II CGTACG 1 cut(s) 303
PfoI TCCNGGA 1 cut(s) 420
PkrI GCNGC 8 cut(s) 474, 532, 537, 641, 644, 726, 970, 1071
PleI GAGTC 2 cut(s) 481, 670
PpsI GAGTC 2 cut(s) 481, 670
Ppu21I YACGTR 2 cut(s) 351, 883
Psp124BI GAGCTC 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 420
PspGI CCWGG 1 cut(s) 420
PspLI CGTACG 1 cut(s) 303
PspN4I GGNNCC 2 cut(s) 248, 509
PspPI GGNCC 2 cut(s) 162, 1165
PstNI CAGNNNCTG 1 cut(s) 1327
PsuI RGATCY 4 cut(s) 274, 507, 770, 817
PvuII CAGCTG 1 cut(s) 642
RsaI GTAC 6 cut(s) 113, 305, 349, 849, 913, 1451
RsaNI GTAC 6 cut(s) 112, 304, 348, 848, 912, 1450
RseI CAYNNNNRTG 3 cut(s) 9, 212, 323
SacI GAGCTC 1 cut(s) 34
SaqAI TTAA 3 cut(s) 579, 1398, 1474
SatI GCNGC 8 cut(s) 473, 531, 536, 640, 643, 725, 969, 1070
Sau96I GGNCC 2 cut(s) 162, 1165
SchI GAGTC 2 cut(s) 481, 671
ScrFI CCNGG 1 cut(s) 422
SduI GDGCHC 2 cut(s) 34, 251
SfaNI GCATC 2 cut(s) 462, 877
SmiMI CAYNNNNRTG 3 cut(s) 9, 212, 323
SmlI CTYRAG 2 cut(s) 954, 1169
SmoI CTYRAG 2 cut(s) 954, 1169
SpeI ACTAGT 1 cut(s) 373
SspMI CTAG 2 cut(s) 374, 974
SstI GAGCTC 1 cut(s) 34
StyD4I CCNGG 1 cut(s) 420
StyI CCWWGG 2 cut(s) 963, 1437
TaaI ACNGT 4 cut(s) 8, 347, 677, 1424
TaiI ACGT 5 cut(s) 172, 216, 305, 353, 885
TaqI TCGA 9 cut(s) 28, 89, 261, 290, 314, 333, 456, 624, 660
TatI WGTACW 1 cut(s) 111
TauI GCSGC 3 cut(s) 475, 538, 971
TfiI GAWTC 5 cut(s) 119, 391, 463, 477, 1430
Tru1I TTAA 3 cut(s) 579, 1398, 1474
Tru9I TTAA 3 cut(s) 579, 1398, 1474
TscAI CASTG 3 cut(s) 411, 726, 1027
TseFI GTSAC 4 cut(s) 280, 358, 484, 1022
TseI GCWGC 5 cut(s) 530, 639, 642, 724, 1069
Tsp45I GTSAC 4 cut(s) 280, 358, 484, 1022
TspDTI ATGAA 4 cut(s) 284, 576, 979, 987
TspGWI ACGGA 3 cut(s) 645, 844, 1247
TspRI CASTG 3 cut(s) 411, 726, 1027
XapI RAATTY 3 cut(s) 78, 711, 1077
XceI RCATGY 1 cut(s) 695
XcmI CCANNNNNNNNNTGG 1 cut(s) 764
XspI CTAG 2 cut(s) 374, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.