Prupe.1G336200_v2.0.a1

U-box domain-containing protein 21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001443
Physical Location & Seq
Reverse (-)
31718418 .. 31719761
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G336200.1

Sequence Viewer

Length: 1344 bp
ATGACTTTGTCATGGAGAAGGCTGCGGCCCGGCCGCCGTGCCTCCAAGGAGCTGCAGCTAGGCGAGCCCGCCGACGTGGAGATGGAGCTGACCATACCAAACCATTTCCGCTGCCCCATCTCACTAGACCTCATGAAAGATCCGGTCACCTTGTCCACTGGAATTACGTACGACCGCCAGAGCATAGAGACGTGGATCGAAGCCGGAAACCTGACGTGCCCAATTACCAACCAAGCCCTCACATGTCTCGAGCCCATTCCCAACCACACCATCCTCAAGATGATTCAAGATTGGTGCGTTCAGAAAAAGTCTTTCGGCATTGAACGCATTCCCACCCCTCGCATCCCAATCACTTCTCTTCAGGTCTCCGAGATTCTCTCCAGAATTACTACAGCAGCAGCCAGCCATGGTCAAAAGACGGATGATTGCAGAGATTTGGTGCGGAAGATCAAGGCCTTGACAAAAGAAAGCGAGCGCAACAAGCGGTGCGTTGTTGCTAGTGGCACGGGGAGCGTTTTAGCCGCTGCTTTTGCTGCACTTTCATCCTCTCAGCGTCAAAACGTTGCCGTCTTGGAGGAAATCTTGTCGGCTTTAACTTTAGTTTTCCCACTGGATGGGGAGGCCTGCTCTTATCTTGGCTCGGCTGCTTCATTGCATTGCATGGTGTGGTTTTTGGAAAGTGGAGATTTGTCCCACAGAAGAAACGCAGCTTTGGTGCTCAAAGAGTCTCTTTCTTCCGATCATCAAAAGATTGATGCTTTGCTGGAGATCGAAGGAGCGTTAGAGGCACTGGTAAAGTTGATCAAAGAACCCGTTTGTCCTACTTCAACAAAAGCCTCCTTGGTCGTCATGTACAACATGGTCACCAATAATTTCACGTCGTCTCTTTCGAAAGATAAAATCAAAGAGAGACTTGTGGAGATGGGATTGGTCTCGTTGCTCTCGGAAATCCTTGTGGATGCCGAACGAAGCATTTGCGAGAAGGCGTTGGGGGTTCTTGATGCACTTTGCGGAAGCAAGGGAGGGAGGGAAAAGGCCTACCATCATGCTTTGACTATGCCCGTTGTGGTGAAGAAGATACTTCGGGTTTCAGATTTGGCGACTGAGTTTTCGGTATCCATTCTTTGGAAGCTGTGCAAGAATGAGGGGAGGGAAGATGGAGGGGTGCTTGTGGAGGTCCTTCAAGTGGGTGCCTTTCAGAAGCTGTTGTTGCTCTTGCAATTAGGGTGTGCGGAGAGGACCAAAGACAAGGCCACCGAGTTGTTGAAGATGTTGAATATTCACAGGGAGAGGTTGGAGTGCATTGATTCTACGGATTTTAAGGCGCTCAAGAGGTCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

448

Amino Acids

49.41

Weight (kDa)

8.13

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1190
AccB7I CCANNNNNTGG 2 cut(s) 614, 1125
AclI AACGTT 1 cut(s) 561
AclWI GGATC 2 cut(s) 134, 203
AcoI YGGCCR 1 cut(s) 31
AcuI CTGAAG 1 cut(s) 344
AfaI GTAC 2 cut(s) 170, 854
AfiI CCNNNNNNNGG 3 cut(s) 614, 1125, 1186
AflIII ACRYGT 1 cut(s) 242
AgsI TTSAA 6 cut(s) 287, 323, 828, 1184, 1267, 1276
AjiI CACGTC 4 cut(s) 76, 192, 216, 879
AluBI AGCT 6 cut(s) 52, 58, 88, 710, 1132, 1204
AluI AGCT 6 cut(s) 52, 58, 88, 710, 1132, 1204
Alw21I GWGCWC 1 cut(s) 720
Alw26I GTCTC 7 cut(s) 182, 251, 370, 732, 888, 904, 937
AlwI GGATC 2 cut(s) 134, 203
AlwNI CAGNNNCTG 1 cut(s) 1204
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 6 cut(s) 26, 31, 453, 621, 1035, 1251
ArsI GACNNNNNNTTYG 2 cut(s) 1093, 1125
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 2 cut(s) 477, 1327
AspS9I GGNCC 3 cut(s) 27, 1177, 1239
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 3 cut(s) 139, 856, 1081
AsuII TTCGAA 1 cut(s) 890
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 2 cut(s) 1177, 1239
BaeGI GKGCMC 1 cut(s) 221
BanI GGYRCC 1 cut(s) 1190
BanII GRGCYC 2 cut(s) 69, 255
Bbv12I GWGCWC 1 cut(s) 720
BccI CCATC 7 cut(s) 76, 125, 278, 608, 916, 1050, 1151
BceAI ACGGC 2 cut(s) 21, 551
BcgI CGANNNNNNTGC 2 cut(s) 957, 991
BciVI GTATCC 1 cut(s) 1126
BclI TGATCA 1 cut(s) 801
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 7 cut(s) 182, 251, 370, 732, 888, 904, 937
BfaI CTAG 3 cut(s) 59, 125, 498
BfmI CTRYAG 2 cut(s) 53, 390
BfoI RGCGCY 1 cut(s) 1328
BfuI GTATCC 1 cut(s) 1126
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 2 cut(s) 1177, 1239
BmeT110I CYCGRG 1 cut(s) 248
BmgBI CACGTC 4 cut(s) 76, 192, 216, 879
BmgT120I GGNCC 3 cut(s) 27, 1177, 1239
BmiI GGNNCC 1 cut(s) 1192
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 4 cut(s) 351, 745, 949, 991
BplI GAGNNNNNCTC 4 cut(s) 362, 394, 611, 643
BpmI CTGGAG 2 cut(s) 364, 785
Bpu14I TTCGAA 1 cut(s) 890
BpuEI CTTGAG 2 cut(s) 260, 1313
BpuMI CCSGG 1 cut(s) 30
BsaAI YACGTR 1 cut(s) 168
BsaI GGTCTC 2 cut(s) 370, 937
BsaJI CCNNGG 3 cut(s) 45, 406, 840
BsaWI WCCGGW 1 cut(s) 142
Bsc4I CCNNNNNNNGG 3 cut(s) 614, 1125, 1186
Bse1I ACTGG 3 cut(s) 163, 615, 795
Bse3DI GCAATG 2 cut(s) 650, 655
BseDI CCNNGG 3 cut(s) 45, 406, 840
BseGI GGATG 6 cut(s) 270, 342, 427, 542, 619, 964
BseLI CCNNNNNNNGG 3 cut(s) 614, 1125, 1186
BseMI GCAATG 2 cut(s) 650, 655
BseMII CTCAG 2 cut(s) 563, 1095
BseNI ACTGG 3 cut(s) 163, 615, 795
BseSI GKGCMC 1 cut(s) 221
BseX3I CGGCCG 1 cut(s) 31
BsgI GTGCAG 1 cut(s) 519
Bsh1285I CGRYCG 2 cut(s) 34, 175
BshFI GGCC 6 cut(s) 28, 33, 455, 623, 1037, 1253
BshNI GGYRCC 1 cut(s) 1190
BsiEI CGRYCG 2 cut(s) 34, 175
BsiHKAI GWGCWC 1 cut(s) 720
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 3 cut(s) 30, 143, 204
BsiWI CGTACG 1 cut(s) 168
BslFI GGGAC 1 cut(s) 676
BslI CCNNNNNNNGG 3 cut(s) 614, 1125, 1186
BsmAI GTCTC 7 cut(s) 182, 251, 370, 732, 888, 904, 937
BsmBI CGTCTC 2 cut(s) 182, 888
BsmFI GGGAC 1 cut(s) 676
BsmI GAATGC 1 cut(s) 327
BsnI GGCC 6 cut(s) 28, 33, 455, 623, 1037, 1253
Bso31I GGTCTC 2 cut(s) 370, 937
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 890
Bsp1286I GDGCHC 4 cut(s) 69, 221, 255, 720
Bsp1407I TGTACA 1 cut(s) 852
Bsp143I GATC 6 cut(s) 139, 195, 447, 739, 768, 801
Bsp19I CCATGG 1 cut(s) 406
BspANI GGCC 6 cut(s) 28, 33, 455, 623, 1037, 1253
BspCNI CTCAG 2 cut(s) 562, 1096
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 1192
BspMAI CTGCAG 1 cut(s) 57
BspPI GGATC 2 cut(s) 134, 203
BspT104I TTCGAA 1 cut(s) 890
BspT107I GGYRCC 1 cut(s) 1190
BspTNI GGTCTC 2 cut(s) 370, 937
BsrDI GCAATG 2 cut(s) 650, 655
BsrGI TGTACA 1 cut(s) 852
BsrI ACTGG 3 cut(s) 163, 615, 795
BssECI CCNNGG 3 cut(s) 45, 406, 840
BssMI GATC 6 cut(s) 139, 195, 447, 739, 768, 801
BssT1I CCWWGG 3 cut(s) 45, 406, 840
Bst6I CTCTTC 1 cut(s) 363
BstAUI TGTACA 1 cut(s) 852
BstBAI YACGTR 1 cut(s) 168
BstBI TTCGAA 1 cut(s) 890
BstC8I GCNNGC 5 cut(s) 65, 69, 403, 473, 625
BstDEI CTNAG 2 cut(s) 549, 1104
BstDSI CCRYGG 1 cut(s) 406
BstEII GGTNACC 2 cut(s) 145, 862
BstF5I GGATG 6 cut(s) 270, 342, 427, 542, 619, 964
BstH2I RGCGCY 1 cut(s) 1328
BstHHI GCGC 2 cut(s) 477, 1327
BstKTI GATC 6 cut(s) 142, 198, 450, 742, 771, 804
BstMAI GTCTC 7 cut(s) 182, 251, 370, 732, 888, 904, 937
BstMBI GATC 6 cut(s) 139, 195, 447, 739, 768, 801
BstMCI CGRYCG 2 cut(s) 34, 175
BstMWI GCNNNNNNNGC 8 cut(s) 64, 324, 481, 510, 530, 533, 785, 1210
BstNSI RCATGY 1 cut(s) 246
BstPI GGTNACC 2 cut(s) 145, 862
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 2 cut(s) 53, 390
BstSLI GKGCMC 1 cut(s) 221
BstSNI TACGTA 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BstZI CGGCCG 1 cut(s) 31
BsuI GTATCC 1 cut(s) 1126
BsuRI GGCC 6 cut(s) 28, 33, 455, 623, 1037, 1253
BtgI CCRYGG 1 cut(s) 406
BtrI CACGTC 4 cut(s) 76, 192, 216, 879
BtsCI GGATG 6 cut(s) 270, 342, 427, 542, 619, 964
BtsIMutI CAGTG 3 cut(s) 156, 608, 788
Cac8I GCNNGC 5 cut(s) 65, 69, 403, 473, 625
CaiI CAGNNNCTG 1 cut(s) 1204
CciI TCATGA 1 cut(s) 132
CfoI GCGC 2 cut(s) 477, 1327
Cfr13I GGNCC 3 cut(s) 27, 1177, 1239
CseI GACGC 1 cut(s) 542
Csp6I GTAC 2 cut(s) 169, 853
CviAII CATG 8 cut(s) 12, 133, 243, 407, 661, 850, 859, 1046
CviQI GTAC 2 cut(s) 169, 853
DdeI CTNAG 2 cut(s) 549, 1104
DpnI GATC 6 cut(s) 141, 197, 449, 741, 770, 803
DpnII GATC 6 cut(s) 139, 195, 447, 739, 768, 801
EaeI YGGCCR 1 cut(s) 31
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
EclXI CGGCCG 1 cut(s) 31
Eco105I TACGTA 1 cut(s) 168
Eco130I CCWWGG 3 cut(s) 45, 406, 840
Eco147I AGGCCT 3 cut(s) 455, 623, 1037
Eco24I GRGCYC 2 cut(s) 69, 255
Eco31I GGTCTC 2 cut(s) 370, 937
Eco47I GGWCC 2 cut(s) 1177, 1239
Eco52I CGGCCG 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 344
Eco88I CYCGRG 1 cut(s) 248
Eco91I GGTNACC 2 cut(s) 145, 862
EcoO109I RGGNCCY 1 cut(s) 1177
EcoO65I GGTNACC 2 cut(s) 145, 862
EcoT14I CCWWGG 3 cut(s) 45, 406, 840
EcoT38I GRGCYC 2 cut(s) 69, 255
ErhI CCWWGG 3 cut(s) 45, 406, 840
Esp3I CGTCTC 2 cut(s) 182, 888
FaeI CATG 8 cut(s) 15, 136, 246, 410, 664, 853, 862, 1049
FalI AAGNNNNNCTT 4 cut(s) 714, 746, 897, 929
FaqI GGGAC 1 cut(s) 676
FatI CATG 8 cut(s) 11, 132, 242, 406, 660, 849, 858, 1045
FauI CCCGC 1 cut(s) 76
FbaI TGATCA 1 cut(s) 801
FokI GGATG 6 cut(s) 257, 329, 434, 529, 626, 971
FriOI GRGCYC 2 cut(s) 69, 255
FspBI CTAG 3 cut(s) 59, 125, 498
GlaI GCGC 2 cut(s) 476, 1326
GsuI CTGGAG 2 cut(s) 364, 785
HaeII RGCGCY 1 cut(s) 1328
HaeIII GGCC 6 cut(s) 28, 33, 455, 623, 1037, 1253
HapII CCGG 3 cut(s) 30, 143, 204
HgaI GACGC 1 cut(s) 542
HhaI GCGC 2 cut(s) 477, 1327
Hin1II CATG 8 cut(s) 15, 136, 246, 410, 664, 853, 862, 1049
Hin6I GCGC 2 cut(s) 475, 1325
HinP1I GCGC 2 cut(s) 475, 1325
HinfI GANTC 4 cut(s) 283, 373, 725, 1307
HpaII CCGG 3 cut(s) 30, 143, 204
HphI GGTGA 3 cut(s) 139, 856, 1081
Hpy166II GTNNAC 1 cut(s) 156
Hpy188I TCNGA 6 cut(s) 303, 370, 739, 946, 1093, 1200
Hpy188III TCNNGA 7 cut(s) 133, 248, 277, 287, 381, 998, 1330
Hpy8I GTNNAC 1 cut(s) 156
Hpy99I CGWCG 2 cut(s) 77, 883
HpyAV CCTTC 4 cut(s) 12, 767, 976, 1190
HpyCH4IV ACGT 6 cut(s) 75, 167, 191, 215, 561, 878
HpyCH4V TGCA 9 cut(s) 55, 429, 536, 655, 660, 1004, 1137, 1219, 1302
HpyF10VI GCNNNNNNNGC 8 cut(s) 64, 324, 481, 510, 530, 533, 785, 1210
HpyF3I CTNAG 2 cut(s) 549, 1104
HpySE526I ACGT 6 cut(s) 75, 167, 191, 215, 561, 878
Hsp92II CATG 8 cut(s) 15, 136, 246, 410, 664, 853, 862, 1049
HspAI GCGC 2 cut(s) 475, 1325
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 6 cut(s) 139, 195, 447, 739, 768, 801
LmnI GCTCC 4 cut(s) 49, 85, 510, 776
LweI GCATC 4 cut(s) 351, 745, 949, 991
MaeI CTAG 3 cut(s) 59, 125, 498
MaeII ACGT 6 cut(s) 75, 167, 191, 215, 561, 878
MaeIII GTNAC 2 cut(s) 145, 862
MalI GATC 6 cut(s) 141, 197, 449, 741, 770, 803
MboI GATC 6 cut(s) 139, 195, 447, 739, 768, 801
MboII GAAGA 8 cut(s) 350, 457, 711, 726, 1084, 1087, 1166, 1279
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 4 cut(s) 69, 221, 255, 720
MluCI AATT 5 cut(s) 162, 222, 384, 871, 1220
MlyI GAGTC 1 cut(s) 734
MmeI TCCRAC 1 cut(s) 1275
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 2 cut(s) 593, 1320
MspA1I CMGCKG 2 cut(s) 111, 524
MspI CCGG 3 cut(s) 30, 143, 204
MspR9I CCNGG 1 cut(s) 30
Mva1269I GAATGC 1 cut(s) 327
MwoI GCNNNNNNNGC 8 cut(s) 64, 324, 481, 510, 530, 533, 785, 1210
NciI CCSGG 1 cut(s) 30
NcoI CCATGG 1 cut(s) 406
NdeII GATC 6 cut(s) 139, 195, 447, 739, 768, 801
NlaIII CATG 8 cut(s) 15, 136, 246, 410, 664, 853, 862, 1049
NlaIV GGNNCC 1 cut(s) 1192
NmeAIII GCCGAG 1 cut(s) 620
NmuCI GTSAC 2 cut(s) 145, 862
NspI RCATGY 1 cut(s) 246
NspV TTCGAA 1 cut(s) 890
PaeR7I CTCGAG 1 cut(s) 248
PagI TCATGA 1 cut(s) 132
PceI AGGCCT 3 cut(s) 455, 623, 1037
PciI ACATGT 1 cut(s) 242
PcsI WCGNNNNNNNCGW 1 cut(s) 887
PctI GAATGC 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 3 cut(s) 283, 373, 1307
Pfl23II CGTACG 1 cut(s) 168
PflFI GACNNNGTC 1 cut(s) 7
PflMI CCANNNNNTGG 2 cut(s) 614, 1125
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
Ppu21I YACGTR 1 cut(s) 168
PpuMI RGGWCCY 1 cut(s) 1177
PscI ACATGT 1 cut(s) 242
Psp1406I AACGTT 1 cut(s) 561
Psp5II RGGWCCY 1 cut(s) 1177
PspEI GGTNACC 2 cut(s) 145, 862
PspLI CGTACG 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 1192
PspPI GGNCC 3 cut(s) 27, 1177, 1239
PspPPI RGGWCCY 1 cut(s) 1177
PstI CTGCAG 1 cut(s) 57
PstNI CAGNNNCTG 1 cut(s) 1204
PsuI RGATCY 1 cut(s) 139
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 2 cut(s) 170, 854
RsaNI GTAC 2 cut(s) 169, 853
SaqAI TTAA 2 cut(s) 593, 1320
Sau3AI GATC 6 cut(s) 139, 195, 447, 739, 768, 801
Sau96I GGNCC 3 cut(s) 27, 1177, 1239
SchI GAGTC 1 cut(s) 734
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 4 cut(s) 69, 221, 255, 720
SfaNI GCATC 4 cut(s) 351, 745, 949, 991
SfcI CTRYAG 2 cut(s) 53, 390
Sfr274I CTCGAG 1 cut(s) 248
SfuI TTCGAA 1 cut(s) 890
SinI GGWCC 2 cut(s) 1177, 1239
SlaI CTCGAG 1 cut(s) 248
SmlI CTYRAG 3 cut(s) 248, 275, 1328
SmoI CTYRAG 3 cut(s) 248, 275, 1328
SnaBI TACGTA 1 cut(s) 168
Sse9I AATT 5 cut(s) 162, 222, 384, 871, 1220
SseBI AGGCCT 3 cut(s) 455, 623, 1037
SspI AATATT 1 cut(s) 1279
SspMI CTAG 3 cut(s) 59, 125, 498
StuI AGGCCT 3 cut(s) 455, 623, 1037
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 3 cut(s) 45, 406, 840
TaiI ACGT 6 cut(s) 78, 170, 194, 218, 564, 881
TaqI TCGA 4 cut(s) 198, 249, 771, 890
TasI AATT 5 cut(s) 162, 222, 384, 871, 1220
TatI WGTACW 1 cut(s) 852
TauI GCSGC 3 cut(s) 28, 36, 524
TfiI GAWTC 3 cut(s) 283, 373, 1307
Tru1I TTAA 2 cut(s) 593, 1320
Tru9I TTAA 2 cut(s) 593, 1320
TscAI CASTG 3 cut(s) 163, 615, 795
TseFI GTSAC 2 cut(s) 145, 862
Tsp45I GTSAC 2 cut(s) 145, 862
TspDTI ATGAA 3 cut(s) 149, 531, 639
TspGWI ACGGA 2 cut(s) 434, 1328
TspRI CASTG 3 cut(s) 163, 615, 795
Tth111I GACNNNGTC 1 cut(s) 7
Van91I CCANNNNNTGG 2 cut(s) 614, 1125
VpaK11BI GGWCC 2 cut(s) 1177, 1239
XceI RCATGY 1 cut(s) 246
XhoI CTCGAG 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 3 cut(s) 59, 125, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.