pycom14g20140

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001443
Physical Location & Seq
Reverse (-)
21245223 .. 21246557
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g20140.1

Sequence Viewer

Length: 1335 bp
ATGGTTTTAGGCTGGGGAAGGAGGAGAACCGGCCACCGTGCAGCGAAAAACCAGCCGGGGGACCTCGACATGGAAGCCCTGATCCCGAACCATTTCCTCTGCCCGATATCGCTTGACTTGATGAAGGATCCGGTCACATTGTCATCCGGAATAACCTACGATAGACAGAGCATCGACAAGTGGCTCGAAGGCGGGAACTTCACTTGCCCTGTGACAAATCAGTTGTTGAGAAGCTTTGATCAGATTCCCAACCATAGTCTCAGAAAAATGATTCAAGAATGGTGCACCGAAAACAAGCGCTATGGAATTGAACGTGTTCCAACTCCGCGAATTCCCGTCATGCCAATTGAGGTCTCCGAGATTCTTTTCAGTTTAGCGGACTCAGCTCGACGTTTGGATCACCAAGGGTGCGTCGAATGTGTGCGCAGAATCAAGAAATGGGCGGCCGAGAGCGAGCGAAACAAGCGCTGTATATTGGAGAATGGGGCTGCATCTGTACTCGCTGCAGCCTTTGATTCTTTCGCAAGTGATTGCGAAAGGAGAAATGCAACTGTGTGTGAAGAATTACTGTCTGCGCTGAGTTGGATGCTTCCGGCTTTCGATGAAGAGTCACACAAACACTTGGGATCCAAGGCATCGTTGCATTGCATGGTTTGGTTTCTCAAGGAAAGTGATGATCTTTCGGTGAAGCAAAACTCCATTCTAGCATTGAAAGAGCTTCTTTCTTGCACTGATCGCACAAAACATGTTGAAGCGTTGGTGGAGATTGGTGGGGTGAATGACATTCTTTTCGAGCTCATCAGAGAGAAAATTTCGCCGGCGATTACCAAGGCATCTCTGACGGTAGTTTTGTACTTGATTTCATCATCTTCTTCCGCCGGTGAAAAGATCGAGTTAGCATTTTTGGAGATGGGATTGGTTTCGGTATTGCTGGAAATGCTTGTGGACTCGGAAAAGGGCGTAAGCGAGAGGGCATTGGCGGTTATCGACAGTCTTTGTGATTGCGAAGAAGGGAGGGAAAAGGCGTATGCTGATGCGCTAACTATACCAGTTTTAGTGAAGAAAATTTTGAGAATATCGGAAATGGCAACTGAGTATTCGATTTCAGCGATTTGGAAGCTGTGTAAATGTGCAAGCAGGCAAGATGAAAGAGTGCTGGTTGAGGCCCTTCAAGTCGGTACATTTCAGAAGCTCTTGTTGGTTTTACAGGTTCGTTGCGGGGATGATCACACGAAGGAGAAGACGAACGAGCTTCTGAAGCTCTTGAATCCTTACAGAGCTGGATTGGAATGCATAGAGTCCGTAGATTTTAAGAGTATTAGAAGGTCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

445

Amino Acids

49.86

Weight (kDa)

5.96

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 28 - 99 2.1e-22 U-box domain
ARM_PUB PF25598 118 - 439 4.2e-65 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 425
AccII CGCG 1 cut(s) 328
AccIII TCCGGA 1 cut(s) 146
AciI CCGC 7 cut(s) 192, 326, 377, 443, 876, 980, 1218
AclWI GGATC 6 cut(s) 76, 122, 135, 405, 621, 634
AcoI YGGCCR 2 cut(s) 31, 444
AcsI RAATTY 3 cut(s) 330, 810, 1065
AcuI CTGAAG 1 cut(s) 1277
AfaI GTAC 3 cut(s) 498, 854, 1180
AfeI AGCGCT 2 cut(s) 299, 467
AfiI CCNNNNNNNGG 2 cut(s) 58, 70
AflIII ACRYGT 2 cut(s) 313, 745
AgsI TTSAA 6 cut(s) 275, 311, 712, 752, 1172, 1267
AjuI GAANNNNNNNTTGG 2 cut(s) 1181, 1213
AluBI AGCT 9 cut(s) 234, 386, 718, 796, 1120, 1192, 1252, 1261, 1280
AluI AGCT 9 cut(s) 234, 386, 718, 796, 1120, 1192, 1252, 1261, 1280
Alw21I GWGCWC 2 cut(s) 287, 798
Alw26I GTCTC 2 cut(s) 263, 358
Alw44I GTGCAC 1 cut(s) 283
AlwI GGATC 6 cut(s) 76, 122, 135, 405, 621, 634
Aor13HI TCCGGA 1 cut(s) 146
Aor51HI AGCGCT 2 cut(s) 299, 467
AoxI GGCC 3 cut(s) 31, 444, 1164
ApaLI GTGCAC 1 cut(s) 283
ApeKI GCWGC 4 cut(s) 41, 488, 503, 506
ApoI RAATTY 3 cut(s) 330, 810, 1065
ArsI GACNNNNNNTTYG 4 cut(s) 773, 805, 832, 864
Asp700I GAANNNNTTC 2 cut(s) 92, 315
AspLEI GCGC 5 cut(s) 300, 426, 468, 577, 1039
AspS9I GGNCC 2 cut(s) 61, 1165
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 4 cut(s) 392, 697, 787, 893
AvaII GGWCC 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 287
BamHI GGATCC 2 cut(s) 127, 626
BanII GRGCYC 1 cut(s) 798
BbsI GAAGAC 1 cut(s) 1247
Bbv12I GWGCWC 2 cut(s) 287, 798
BbvI GCAGC 4 cut(s) 53, 475, 490, 518
BccI CCATC 1 cut(s) 904
BclI TGATCA 2 cut(s) 238, 1225
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 2 cut(s) 263, 358
BfaI CTAG 1 cut(s) 704
BfmI CTRYAG 1 cut(s) 504
BfoI RGCGCY 2 cut(s) 301, 469
BisI GCNGC 5 cut(s) 42, 444, 489, 504, 507
BlsI GCNGC 5 cut(s) 43, 445, 490, 505, 508
Bme1390I CCNGG 1 cut(s) 57
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 2 cut(s) 61, 1165
BmiI GGNNCC 3 cut(s) 62, 129, 628
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 6 cut(s) 180, 500, 576, 644, 842, 1024
BpiI GAAGAC 1 cut(s) 1247
BpuEI CTTGAG 1 cut(s) 647
BpuMI CCSGG 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 358
BsaJI CCNNGG 4 cut(s) 56, 403, 630, 828
BsaWI WCCGGW 2 cut(s) 130, 146
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 70
Bse118I RCCGGY 3 cut(s) 29, 817, 878
Bse1I ACTGG 1 cut(s) 1049
Bse3DI GCAATG 1 cut(s) 643
BseAI TCCGGA 1 cut(s) 146
BseDI CCNNGG 4 cut(s) 56, 403, 630, 828
BseGI GGATG 3 cut(s) 143, 591, 1228
BseLI CCNNNNNNNGG 2 cut(s) 58, 70
BseMI GCAATG 1 cut(s) 643
BseMII CTCAG 4 cut(s) 274, 396, 569, 1083
BseNI ACTGG 1 cut(s) 1049
BseRI GAGGAG 1 cut(s) 37
BseSI GKGCMC 1 cut(s) 287
BseX3I CGGCCG 1 cut(s) 444
BseXI GCAGC 4 cut(s) 53, 475, 490, 518
BseYI CCCAGC 1 cut(s) 12
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 328
Bsh1285I CGRYCG 1 cut(s) 447
BshFI GGCC 3 cut(s) 33, 446, 1166
BsiEI CGRYCG 1 cut(s) 447
BsiHKAI GWGCWC 2 cut(s) 287, 798
BsiSI CCGG 7 cut(s) 30, 56, 131, 147, 593, 818, 879
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 2 cut(s) 58, 70
BsmAI GTCTC 2 cut(s) 263, 358
BsmFI GGGAC 1 cut(s) 74
BsmI GAATGC 1 cut(s) 1295
BsnI GGCC 3 cut(s) 33, 446, 1166
Bso31I GGTCTC 1 cut(s) 358
Bsp1286I GDGCHC 2 cut(s) 287, 798
Bsp13I TCCGGA 1 cut(s) 146
Bsp143I GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
BspACI CCGC 7 cut(s) 192, 326, 377, 443, 876, 980, 1218
BspANI GGCC 3 cut(s) 33, 446, 1166
BspCNI CTCAG 4 cut(s) 273, 395, 570, 1084
BspEI TCCGGA 1 cut(s) 146
BspFNI CGCG 1 cut(s) 328
BspLI GGNNCC 3 cut(s) 62, 129, 628
BspMAI CTGCAG 1 cut(s) 508
BspPI GGATC 6 cut(s) 76, 122, 135, 405, 621, 634
BspTNI GGTCTC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 643
BsrFI RCCGGY 3 cut(s) 29, 817, 878
BsrI ACTGG 1 cut(s) 1049
BssAI RCCGGY 3 cut(s) 29, 817, 878
BssECI CCNNGG 4 cut(s) 56, 403, 630, 828
BssMI GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
BssT1I CCWWGG 3 cut(s) 403, 630, 828
Bst4CI ACNGT 5 cut(s) 38, 553, 570, 844, 992
Bst6I CTCTTC 1 cut(s) 600
BstC8I GCNNGC 4 cut(s) 455, 819, 1135, 1139
BstDEI CTNAG 4 cut(s) 260, 382, 578, 1092
BstF5I GGATG 3 cut(s) 143, 591, 1228
BstFNI CGCG 1 cut(s) 328
BstH2I RGCGCY 2 cut(s) 301, 469
BstHHI GCGC 5 cut(s) 300, 426, 468, 577, 1039
BstKTI GATC 9 cut(s) 84, 130, 241, 400, 629, 679, 736, 891, 1228
BstMAI GTCTC 2 cut(s) 263, 358
BstMBI GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
BstMCI CGRYCG 1 cut(s) 447
BstMWI GCNNNNNNNGC 5 cut(s) 383, 463, 735, 937, 1258
BstNSI RCATGY 1 cut(s) 749
BstSCI CCNGG 1 cut(s) 55
BstSFI CTRYAG 1 cut(s) 504
BstSLI GKGCMC 1 cut(s) 287
BstUI CGCG 1 cut(s) 328
BstV1I GCAGC 4 cut(s) 53, 475, 490, 518
BstV2I GAAGAC 1 cut(s) 1247
BstX2I RGATCY 2 cut(s) 127, 626
BstYI RGATCY 2 cut(s) 127, 626
BstZI CGGCCG 1 cut(s) 444
BsuRI GGCC 3 cut(s) 33, 446, 1166
BtsCI GGATG 3 cut(s) 143, 591, 1228
BtsIMutI CAGTG 1 cut(s) 729
Cac8I GCNNGC 4 cut(s) 455, 819, 1135, 1139
CfoI GCGC 5 cut(s) 300, 426, 468, 577, 1039
Cfr10I RCCGGY 3 cut(s) 29, 817, 878
Cfr13I GGNCC 2 cut(s) 61, 1165
CseI GACGC 1 cut(s) 400
Csp6I GTAC 3 cut(s) 497, 853, 1179
CviAII CATG 4 cut(s) 70, 340, 649, 746
CviQI GTAC 3 cut(s) 497, 853, 1179
DdeI CTNAG 4 cut(s) 260, 382, 578, 1092
DpnI GATC 9 cut(s) 83, 129, 240, 399, 628, 678, 735, 890, 1227
DpnII GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
EaeI YGGCCR 2 cut(s) 31, 444
EagI CGGCCG 1 cut(s) 444
Eam1104I CTCTTC 1 cut(s) 600
EarI CTCTTC 1 cut(s) 600
EciI GGCGGA 1 cut(s) 865
Ecl136II GAGCTC 1 cut(s) 796
EclXI CGGCCG 1 cut(s) 444
Eco130I CCWWGG 3 cut(s) 403, 630, 828
Eco24I GRGCYC 1 cut(s) 798
Eco31I GGTCTC 1 cut(s) 358
Eco32I GATATC 1 cut(s) 108
Eco47I GGWCC 1 cut(s) 61
Eco47III AGCGCT 2 cut(s) 299, 467
Eco52I CGGCCG 1 cut(s) 444
Eco53kI GAGCTC 1 cut(s) 796
Eco57I CTGAAG 1 cut(s) 1277
EcoICRI GAGCTC 1 cut(s) 796
EcoO109I RGGNCCY 2 cut(s) 61, 1165
EcoRI GAATTC 1 cut(s) 330
EcoRV GATATC 1 cut(s) 108
EcoT14I CCWWGG 3 cut(s) 403, 630, 828
EcoT22I ATGCAT 1 cut(s) 1295
EcoT38I GRGCYC 1 cut(s) 798
ErhI CCWWGG 3 cut(s) 403, 630, 828
FaeI CATG 4 cut(s) 73, 343, 652, 749
FalI AAGNNNNNCTT 2 cut(s) 705, 737
FaqI GGGAC 1 cut(s) 74
FatI CATG 4 cut(s) 69, 339, 648, 745
FauI CCCGC 2 cut(s) 185, 1211
FbaI TGATCA 2 cut(s) 238, 1225
Fnu4HI GCNGC 5 cut(s) 42, 444, 489, 504, 507
FokI GGATG 3 cut(s) 130, 598, 1235
FriOI GRGCYC 1 cut(s) 798
Fsp4HI GCNGC 5 cut(s) 42, 444, 489, 504, 507
FspBI CTAG 1 cut(s) 704
FspI TGCGCA 1 cut(s) 425
GlaI GCGC 5 cut(s) 299, 425, 467, 576, 1038
GluI GCNGC 5 cut(s) 42, 444, 489, 504, 507
GsaI CCCAGC 1 cut(s) 16
HaeII RGCGCY 2 cut(s) 301, 469
HaeIII GGCC 3 cut(s) 33, 446, 1166
HapII CCGG 7 cut(s) 30, 56, 131, 147, 593, 818, 879
HgaI GACGC 1 cut(s) 400
HhaI GCGC 5 cut(s) 300, 426, 468, 577, 1039
Hin1II CATG 4 cut(s) 73, 343, 652, 749
Hin6I GCGC 5 cut(s) 298, 424, 466, 575, 1037
HinP1I GCGC 5 cut(s) 298, 424, 466, 575, 1037
HindIII AAGCTT 1 cut(s) 232
HpaII CCGG 7 cut(s) 30, 56, 131, 147, 593, 818, 879
HphI GGTGA 4 cut(s) 392, 697, 787, 893
Hpy166II GTNNAC 2 cut(s) 285, 946
Hpy188I TCNGA 9 cut(s) 243, 263, 358, 803, 840, 952, 1081, 1188, 1257
Hpy188III TCNNGA 5 cut(s) 85, 147, 275, 433, 1264
Hpy8I GTNNAC 2 cut(s) 285, 946
Hpy99I CGWCG 2 cut(s) 393, 416
HpyAV CCTTC 7 cut(s) 12, 118, 182, 1004, 1178, 1228, 1317
HpyCH4III ACNGT 5 cut(s) 38, 553, 570, 844, 992
HpyCH4IV ACGT 2 cut(s) 313, 391
HpyF10VI GCNNNNNNNGC 5 cut(s) 383, 463, 735, 937, 1258
HpyF3I CTNAG 4 cut(s) 260, 382, 578, 1092
HpySE526I ACGT 2 cut(s) 313, 391
Hsp92II CATG 4 cut(s) 73, 343, 652, 749
HspAI GCGC 5 cut(s) 298, 424, 466, 575, 1037
Kpn2I TCCGGA 1 cut(s) 146
KroI GCCGGC 1 cut(s) 817
KroNI GCCGGC 1 cut(s) 819
Ksp22I TGATCA 2 cut(s) 238, 1225
Kzo9I GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
Lsp1109I GCAGC 4 cut(s) 53, 475, 490, 518
LweI GCATC 6 cut(s) 180, 500, 576, 644, 842, 1024
MaeI CTAG 1 cut(s) 704
MaeII ACGT 2 cut(s) 313, 391
MaeIII GTNAC 3 cut(s) 133, 211, 609
MalI GATC 9 cut(s) 83, 129, 240, 399, 628, 678, 735, 890, 1227
MboI GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
MboII GAAGA 7 cut(s) 572, 617, 861, 864, 1019, 1072, 1252
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 2 cut(s) 127, 626
MhlI GDGCHC 2 cut(s) 287, 798
MluCI AATT 6 cut(s) 306, 330, 345, 563, 810, 1065
MlyI GAGTC 4 cut(s) 374, 617, 941, 1307
MmeI TCCRAC 2 cut(s) 344, 563
MnlI CCTC 7 cut(s) 15, 74, 107, 343, 963, 1008, 1156
Mph1103I ATGCAT 1 cut(s) 1295
MreI CGCCGGCG 1 cut(s) 817
MroI TCCGGA 1 cut(s) 146
MroNI GCCGGC 1 cut(s) 817
MroXI GAANNNNTTC 2 cut(s) 92, 315
MseI TTAA 1 cut(s) 1311
MspI CCGG 7 cut(s) 30, 56, 131, 147, 593, 818, 879
MspR9I CCNGG 1 cut(s) 57
MunI CAATTG 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 1295
MvnI CGCG 1 cut(s) 328
MwoI GCNNNNNNNGC 5 cut(s) 383, 463, 735, 937, 1258
NaeI GCCGGC 1 cut(s) 819
NciI CCSGG 1 cut(s) 57
NdeII GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
NgoMIV GCCGGC 1 cut(s) 817
NlaIII CATG 4 cut(s) 73, 343, 652, 749
NlaIV GGNNCC 3 cut(s) 62, 129, 628
NmeAIII GCCGAG 1 cut(s) 472
NmuCI GTSAC 3 cut(s) 133, 211, 609
NsbI TGCGCA 1 cut(s) 425
NsiI ATGCAT 1 cut(s) 1295
NspI RCATGY 1 cut(s) 749
PciI ACATGT 1 cut(s) 745
PcsI WCGNNNNNNNCGW 1 cut(s) 1106
PctI GAATGC 1 cut(s) 1295
PdiI GCCGGC 1 cut(s) 819
PdmI GAANNNNTTC 2 cut(s) 92, 315
PfeI GAWTC 6 cut(s) 244, 271, 361, 429, 515, 1267
PkrI GCNGC 5 cut(s) 43, 445, 490, 505, 508
PleI GAGTC 4 cut(s) 374, 616, 941, 1306
PpsI GAGTC 4 cut(s) 374, 616, 941, 1306
PpuMI RGGWCCY 1 cut(s) 61
PscI ACATGT 1 cut(s) 745
Psp124BI GAGCTC 1 cut(s) 798
Psp5II RGGWCCY 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 12
PspN4I GGNNCC 3 cut(s) 62, 129, 628
PspPI GGNCC 2 cut(s) 61, 1165
PspPPI RGGWCCY 1 cut(s) 61
PstI CTGCAG 1 cut(s) 508
PsuI RGATCY 2 cut(s) 127, 626
RsaI GTAC 3 cut(s) 498, 854, 1180
RsaNI GTAC 3 cut(s) 497, 853, 1179
SacI GAGCTC 1 cut(s) 798
SaqAI TTAA 1 cut(s) 1311
SatI GCNGC 5 cut(s) 42, 444, 489, 504, 507
Sau3AI GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
Sau96I GGNCC 2 cut(s) 61, 1165
SchI GAGTC 4 cut(s) 374, 617, 941, 1307
ScrFI CCNGG 1 cut(s) 57
SduI GDGCHC 2 cut(s) 287, 798
SfaNI GCATC 6 cut(s) 180, 500, 576, 644, 842, 1024
SfcI CTRYAG 1 cut(s) 504
SgrAI CRCCGGYG 2 cut(s) 817, 878
SinI GGWCC 1 cut(s) 61
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
Sse9I AATT 6 cut(s) 306, 330, 345, 563, 810, 1065
SsiI CCGC 7 cut(s) 192, 326, 377, 443, 876, 980, 1218
SspMI CTAG 1 cut(s) 704
SstI GAGCTC 1 cut(s) 798
StyD4I CCNGG 1 cut(s) 55
StyI CCWWGG 3 cut(s) 403, 630, 828
TaaI ACNGT 5 cut(s) 38, 553, 570, 844, 992
TaiI ACGT 2 cut(s) 316, 394
TasI AATT 6 cut(s) 306, 330, 345, 563, 810, 1065
TatI WGTACW 2 cut(s) 496, 852
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 6 cut(s) 244, 271, 361, 429, 515, 1267
Tru1I TTAA 1 cut(s) 1311
Tru9I TTAA 1 cut(s) 1311
TscAI CASTG 1 cut(s) 736
TseFI GTSAC 3 cut(s) 133, 211, 609
TseI GCWGC 4 cut(s) 41, 488, 503, 506
Tsp45I GTSAC 3 cut(s) 133, 211, 609
TspDTI ATGAA 4 cut(s) 137, 618, 852, 1161
TspGWI ACGGA 1 cut(s) 1291
TspRI CASTG 1 cut(s) 736
VneI GTGCAC 1 cut(s) 283
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 3 cut(s) 330, 810, 1065
XceI RCATGY 1 cut(s) 749
XmnI GAANNNNTTC 2 cut(s) 92, 315
XspI CTAG 1 cut(s) 704
Zsp2I ATGCAT 1 cut(s) 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.