pycom09g17960

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001443
Physical Location & Seq
Forward (+)
18549853 .. 18550710
858 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g17960.1

Sequence Viewer

Length: 858 bp
ATGGCGGAGAACGGAATTGGCGGCTGCTTATCAGCCGTGTTCGAGTCCTTTGCCAGCAGTTCAGCGGACGAATATGTTGAGATATTGGTGGAAATTTTAACTGTGCTACCGTGGATGTTTCCATTGGGTGAAGAAGACCAATCGAAGCTTGGATCTTCAGTGTGTTTGCGATCTTTCATGTCGTTTCTAGAGGGCAAAGATCTAACAGCACGGCAAAACGCGGTTTTGGTACTTAGAAAGTTGTTTTCTTTGGATCAAAAATATGTGGATGCTTTCGGGAAGATTGAAGGAGGCGTTGAAGCGATTTTTGGGATCATTGGAGAGCCTATTTGTCCTCAAGTGACTAAGGCTGCTCTGGCTTGTGTTCTTTTCATGATTTCATCACTGCCATCCGCAGAAACCAGTGATAAATACAGATCAAGATTTGTGGAATTGGGTTTGGTTTCTTTGCTACTAGAAACTCTTGTTGACGCAGAGAAAAGCGTTTCAGAGACAGCTTTGGGGGTTTTGGATGGTCTCTGTGATTGCAAACGAGGGAGAGAAAAGGCCCTCGAGAATGCTCTCACAATGCCTCTTTTGGTGAAGAAGATTTTGAGGATTTCGGAGTTGGGGACGGAGTTTTCGGTGTCTATAATCTGGAAGCTGTGCACCATTGAAACCCAGGAAAGCTTTTTGATTGAGGCGCTTCAAGTGGGCGCGTTTCAGAAGCTCTTGGTGCTCTTGCAGGTCGGGGTTTGTAATGGAATGAAGGACAAGGTTACTCATCTGCTGAAATTGTTCAATCTGCACATGAGTAAATTGGACTGTGTGGATTCATCCATGAATTTCAAGTACCTCAAGAGGCCATCTTTGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

286

Amino Acids

31.29

Weight (kDa)

5.2

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 1 - 279 1.2e-57 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 715
AccII CGCG 2 cut(s) 221, 698
AciI CCGC 5 cut(s) 5, 21, 65, 221, 393
AclWI GGATC 3 cut(s) 160, 261, 320
AcsI RAATTY 2 cut(s) 93, 823
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 2 cut(s) 231, 833
AgsI TTSAA 6 cut(s) 287, 299, 656, 689, 781, 829
AjnI CCWGG 1 cut(s) 660
AjuI GAANNNNNNNTTGG 2 cut(s) 291, 323
AluBI AGCT 5 cut(s) 148, 497, 643, 669, 709
AluI AGCT 5 cut(s) 148, 497, 643, 669, 709
Alw21I GWGCWC 2 cut(s) 650, 720
Alw26I GTCTC 2 cut(s) 485, 521
Alw44I GTGCAC 1 cut(s) 646
AlwI GGATC 3 cut(s) 160, 261, 320
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 2 cut(s) 546, 842
ApaLI GTGCAC 1 cut(s) 646
ApeKI GCWGC 2 cut(s) 24, 350
ApoI RAATTY 2 cut(s) 93, 823
ArsI GACNNNNNNTTYG 2 cut(s) 604, 636
Asp700I GAANNNNTTC 1 cut(s) 776
AspLEI GCGC 2 cut(s) 685, 698
AspS9I GGNCC 1 cut(s) 547
AsuHPI GGTGA 2 cut(s) 140, 592
AvaI CYCGRG 1 cut(s) 551
BaeGI GKGCMC 1 cut(s) 650
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 2 cut(s) 650, 720
BbvI GCAGC 2 cut(s) 11, 337
BccI CCATC 3 cut(s) 397, 506, 853
BceAI ACGGC 2 cut(s) 20, 227
BcgI CGANNNNNNTGC 2 cut(s) 32, 66
BciT130I CCWGG 1 cut(s) 662
BcoDI GTCTC 2 cut(s) 485, 521
BfaI CTAG 2 cut(s) 188, 455
BfoI RGCGCY 1 cut(s) 686
BfuAI ACCTGC 1 cut(s) 715
BglII AGATCT 1 cut(s) 199
BisI GCNGC 3 cut(s) 22, 25, 351
BlsI GCNGC 3 cut(s) 23, 26, 352
Bme1390I CCNGG 1 cut(s) 662
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 1 cut(s) 547
BmrFI CCNGG 1 cut(s) 662
BmsI GCATC 1 cut(s) 259
BpiI GAAGAC 1 cut(s) 141
BpuEI CTTGAG 2 cut(s) 321, 821
BsaI GGTCTC 1 cut(s) 521
BsaJI CCNNGG 2 cut(s) 110, 660
Bse1I ACTGG 1 cut(s) 402
BseBI CCWGG 1 cut(s) 662
BseDI CCNNGG 2 cut(s) 110, 660
BseGI GGATG 5 cut(s) 120, 274, 389, 517, 815
BseNI ACTGG 1 cut(s) 402
BseSI GKGCMC 1 cut(s) 650
BseXI GCAGC 2 cut(s) 11, 337
BsgI GTGCAG 1 cut(s) 770
Bsh1236I CGCG 2 cut(s) 221, 698
BshFI GGCC 2 cut(s) 548, 844
BsiHKAI GWGCWC 2 cut(s) 650, 720
BsiHKCI CYCGRG 1 cut(s) 551
BslFI GGGAC 1 cut(s) 625
BsmAI GTCTC 2 cut(s) 485, 521
BsmFI GGGAC 1 cut(s) 625
BsmI GAATGC 1 cut(s) 562
BsnI GGCC 2 cut(s) 548, 844
Bso31I GGTCTC 1 cut(s) 521
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 2 cut(s) 650, 720
Bsp143I GATC 6 cut(s) 152, 170, 199, 253, 312, 416
BspACI CCGC 5 cut(s) 5, 21, 65, 221, 393
BspANI GGCC 2 cut(s) 548, 844
BspFNI CGCG 2 cut(s) 221, 698
BspHI TCATGA 1 cut(s) 372
BspMI ACCTGC 1 cut(s) 715
BspPI GGATC 3 cut(s) 160, 261, 320
BspTNI GGTCTC 1 cut(s) 521
BsrI ACTGG 1 cut(s) 402
BssECI CCNNGG 2 cut(s) 110, 660
BssMI GATC 6 cut(s) 152, 170, 199, 253, 312, 416
Bst2UI CCWGG 1 cut(s) 662
Bst4CI ACNGT 3 cut(s) 103, 111, 806
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 2 cut(s) 233, 345
BstDSI CCRYGG 1 cut(s) 110
BstF5I GGATG 5 cut(s) 120, 274, 389, 517, 815
BstFNI CGCG 2 cut(s) 221, 698
BstH2I RGCGCY 1 cut(s) 686
BstHHI GCGC 2 cut(s) 685, 698
BstKTI GATC 6 cut(s) 155, 173, 202, 256, 315, 419
BstMAI GTCTC 2 cut(s) 485, 521
BstMBI GATC 6 cut(s) 152, 170, 199, 253, 312, 416
BstMWI GCNNNNNNNGC 2 cut(s) 356, 715
BstNI CCWGG 1 cut(s) 662
BstSCI CCNGG 1 cut(s) 660
BstSLI GKGCMC 1 cut(s) 650
BstUI CGCG 2 cut(s) 221, 698
BstV1I GCAGC 2 cut(s) 11, 337
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 2 cut(s) 152, 199
BstYI RGATCY 2 cut(s) 152, 199
BsuRI GGCC 2 cut(s) 548, 844
BtgI CCRYGG 1 cut(s) 110
BtsCI GGATG 5 cut(s) 120, 274, 389, 517, 815
BtsI GCAGTG 1 cut(s) 383
BtsIMutI CAGTG 3 cut(s) 165, 383, 409
BveI ACCTGC 1 cut(s) 715
Cac8I GCNNGC 1 cut(s) 55
CciI TCATGA 1 cut(s) 372
CfoI GCGC 2 cut(s) 685, 698
Cfr13I GGNCC 1 cut(s) 547
CseI GACGC 1 cut(s) 479
Csp6I GTAC 2 cut(s) 230, 832
CspCI CAANNNNNGTGG 2 cut(s) 408, 443
CviAII CATG 4 cut(s) 178, 373, 790, 820
CviQI GTAC 2 cut(s) 230, 832
DdeI CTNAG 2 cut(s) 233, 345
DpnI GATC 6 cut(s) 154, 172, 201, 255, 314, 418
DpnII GATC 6 cut(s) 152, 170, 199, 253, 312, 416
EciI GGCGGA 1 cut(s) 20
Eco31I GGTCTC 1 cut(s) 521
Eco57I CTGAAG 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 551
EcoO109I RGGNCCY 1 cut(s) 547
EcoRII CCWGG 1 cut(s) 660
FaeI CATG 4 cut(s) 181, 376, 793, 823
FaiI YATR 7 cut(s) 75, 179, 264, 374, 632, 791, 821
FaqI GGGAC 1 cut(s) 625
FatI CATG 4 cut(s) 177, 372, 789, 819
Fnu4HI GCNGC 3 cut(s) 22, 25, 351
FokI GGATG 5 cut(s) 127, 281, 376, 524, 802
Fsp4HI GCNGC 3 cut(s) 22, 25, 351
FspBI CTAG 2 cut(s) 188, 455
GlaI GCGC 2 cut(s) 684, 697
GluI GCNGC 3 cut(s) 22, 25, 351
HaeII RGCGCY 1 cut(s) 686
HaeIII GGCC 2 cut(s) 548, 844
HgaI GACGC 1 cut(s) 479
HhaI GCGC 2 cut(s) 685, 698
Hin1II CATG 4 cut(s) 181, 376, 793, 823
Hin6I GCGC 2 cut(s) 683, 696
HinP1I GCGC 2 cut(s) 683, 696
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 2 cut(s) 146, 667
HinfI GANTC 2 cut(s) 44, 812
HphI GGTGA 2 cut(s) 140, 592
Hpy166II GTNNAC 2 cut(s) 469, 648
Hpy188I TCNGA 3 cut(s) 490, 604, 705
Hpy188III TCNNGA 7 cut(s) 188, 277, 373, 420, 553, 637, 838
Hpy8I GTNNAC 2 cut(s) 469, 648
HpyAV CCTTC 2 cut(s) 281, 742
HpyCH4III ACNGT 3 cut(s) 103, 111, 806
HpyCH4V TGCA 4 cut(s) 528, 648, 724, 787
HpyF10VI GCNNNNNNNGC 2 cut(s) 356, 715
HpyF3I CTNAG 2 cut(s) 233, 345
Hsp92II CATG 4 cut(s) 181, 376, 793, 823
HspAI GCGC 2 cut(s) 683, 696
Kzo9I GATC 6 cut(s) 152, 170, 199, 253, 312, 416
LpnPI CCDG 7 cut(s) 67, 341, 415, 622, 647, 674, 710
Lsp1109I GCAGC 2 cut(s) 11, 337
LweI GCATC 1 cut(s) 259
MaeI CTAG 2 cut(s) 188, 455
MaeIII GTNAC 2 cut(s) 340, 757
MalI GATC 6 cut(s) 154, 172, 201, 255, 314, 418
MboI GATC 6 cut(s) 152, 170, 199, 253, 312, 416
MboII GAAGA 6 cut(s) 143, 146, 147, 292, 595, 598
MflI RGATCY 2 cut(s) 152, 199
MhlI GDGCHC 2 cut(s) 650, 720
MluCI AATT 6 cut(s) 15, 93, 431, 773, 797, 823
MlyI GAGTC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 776
MseI TTAA 1 cut(s) 98
MspA1I CMGCKG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 662
Mva1269I GAATGC 1 cut(s) 562
MvaI CCWGG 1 cut(s) 662
MvnI CGCG 2 cut(s) 221, 698
MwoI GCNNNNNNNGC 2 cut(s) 356, 715
NdeII GATC 6 cut(s) 152, 170, 199, 253, 312, 416
NlaIII CATG 4 cut(s) 181, 376, 793, 823
NmuCI GTSAC 1 cut(s) 340
PaeR7I CTCGAG 1 cut(s) 551
PagI TCATGA 1 cut(s) 372
PctI GAATGC 1 cut(s) 562
PdmI GAANNNNTTC 1 cut(s) 776
PfeI GAWTC 1 cut(s) 812
PkrI GCNGC 3 cut(s) 23, 26, 352
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 660
PspGI CCWGG 1 cut(s) 660
PspPI GGNCC 1 cut(s) 547
PsuI RGATCY 2 cut(s) 152, 199
RsaI GTAC 2 cut(s) 231, 833
RsaNI GTAC 2 cut(s) 230, 832
SaqAI TTAA 1 cut(s) 98
SatI GCNGC 3 cut(s) 22, 25, 351
Sau3AI GATC 6 cut(s) 152, 170, 199, 253, 312, 416
Sau96I GGNCC 1 cut(s) 547
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 1 cut(s) 662
SduI GDGCHC 2 cut(s) 650, 720
SetI ASST 8 cut(s) 150, 499, 645, 671, 711, 729, 759, 837
SfaNI GCATC 1 cut(s) 259
Sfr274I CTCGAG 1 cut(s) 551
SlaI CTCGAG 1 cut(s) 551
SmlI CTYRAG 3 cut(s) 336, 551, 836
SmoI CTYRAG 3 cut(s) 336, 551, 836
Sse9I AATT 6 cut(s) 15, 93, 431, 773, 797, 823
SsiI CCGC 5 cut(s) 5, 21, 65, 221, 393
SspMI CTAG 2 cut(s) 188, 455
StyD4I CCNGG 1 cut(s) 660
TaaI ACNGT 3 cut(s) 103, 111, 806
TaqI TCGA 3 cut(s) 42, 143, 552
TasI AATT 6 cut(s) 15, 93, 431, 773, 797, 823
TauI GCSGC 1 cut(s) 24
TfiI GAWTC 1 cut(s) 812
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TscAI CASTG 3 cut(s) 165, 390, 409
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 2 cut(s) 24, 350
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 6 cut(s) 166, 361, 369, 761, 804, 836
TspGWI ACGGA 2 cut(s) 27, 629
TspRI CASTG 3 cut(s) 165, 390, 409
VneI GTGCAC 1 cut(s) 646
XapI RAATTY 2 cut(s) 93, 823
XbaI TCTAGA 1 cut(s) 187
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
XhoI CTCGAG 1 cut(s) 551
XmnI GAANNNNTTC 1 cut(s) 776
XspI CTAG 2 cut(s) 188, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.