MD06G1232800.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001443
Physical Location & Seq
Reverse (-)
36417677 .. 36419011
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1232800.v1.1.491

Sequence Viewer

Length: 1335 bp
ATGGGTTTAGGCTGGATAAGGAGGAGAACCGGCCACCGCGCCGCGAAAAACCAGCCAGGGGACCTCGACATGGAAGCCCTGATCCCGGACCATTTTCTCTGCCCGATATCGCTTGATTTGATGAAGGATCCGGTCACATTGTCATCCGGAATAACCTACGATAGACAGAGCATCGACACCTGGCTCGAAGGCGGGAACTTTACTTGCCCTGTGACAAATCAGGTGTTGAGAAGCTTTGATCAGATTCCCAACCATAGTCTCAGAAAAATGATTCAAGAATGGTGCACCGAAAACAAGCGCTACGGAATTGAACGTGTTCCAACGCCGCGAGTTCCTGTTATGCCGAACGAGGTCTCCGAGATTCTTTTCAGTTTAGCGGGCTCAGCTCGAAATTTGGATCACCAAGGGTGCTTCGAATGTGTGCGCAGAATCAAGAATTGGGCCGCTGAGAGTGAGCGAAACAAGCGGTGCATATTGGAGAACGGGGCTGCATCTGTACTCTCTGCCGCCTTTGATTCTTTTGCAGGCGATTGCGGAAAGAAAAATGCAATTGTGTGCGAAGAATTACTCTCTGCGTTGAGTTGGATGCTTCCGGCTTTCGATGAAGAATCACACAAATACTTGGGATCGAAGGCATCGTTGCATTGCATGGTTTGGTTTCTCAAGGAAACCGATGATCTTTCGGTCAAGCAAAACTCCCTTCTAGCATTGAAACAGCTTCTTTCTTGCGATGATCACACTAAACATGCTGAAGCGTTGGCGGAGATTGGTGGGGTGAATGACATTCTTTTCAAGCTCATCAGAGAGAAAATTTCGCCCGCGATTACCAAGGCTTCTCTGATGGTAGTTTTCTACTTGATTTCATCATCTTCTTCCGCCGGTGAGAAGATCAAGTTATCATTTTTGGAGATGGGATTGGTTTCTGTGTTGCTGGAAATGCTTGTGGACTCGGAAAGGGGCGTAAGCGAGAGGGCATTGGCGGTTATCGACAGTCTTTGTGATTGCGATGAAGGGAGGGAAAAGGCGTACGCTGATGCGCTAACTATACCAGTTTTAGTGAAGAAAATTTTGAGGATATCTGAAATGGCGACTGAGCATTCGATTTCAGCGATTTGGAAGCTGTGTAAATGTGCAAGCAGGCAAGAAGAAAGAGTGCTGGTTGAGGCCCTTCAAGTCGGTACATTTCAGAAGCTCTTGTTGGTTTTACAGGTTCGTTGCGGGGACGATCACACGAAGGAGAAGACGAACGAGCTTCTGAAGCTCTTGAATCCTTACAGAGCTGGATTGGAATGCATTGAGTCTGTAGATTTCAAGAGTATTAGAAGGTCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

445

Amino Acids

49.68

Weight (kDa)

6.12

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 28 - 99 5.2e-23 U-box domain
ARM_PUB PF25598 118 - 439 2e-65 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 206 - 387 8.8e-06 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 425
AccII CGCG 4 cut(s) 39, 44, 328, 821
AccIII TCCGGA 1 cut(s) 146
AclWI GGATC 5 cut(s) 76, 122, 135, 405, 634
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 3 cut(s) 391, 810, 1065
AcuI CTGAAG 2 cut(s) 771, 1277
AfaI GTAC 3 cut(s) 498, 1028, 1180
AfeI AGCGCT 1 cut(s) 299
AfiI CCNNNNNNNGG 3 cut(s) 58, 70, 85
AflIII ACRYGT 1 cut(s) 313
AgsI TTSAA 7 cut(s) 275, 311, 712, 793, 1172, 1267, 1312
AjnI CCWGG 2 cut(s) 55, 179
AjuI GAANNNNNNNTTGG 4 cut(s) 421, 453, 1181, 1213
AloI GAACNNNNNNTCC 2 cut(s) 338, 370
AluBI AGCT 9 cut(s) 234, 386, 718, 796, 1120, 1192, 1252, 1261, 1280
AluI AGCT 9 cut(s) 234, 386, 718, 796, 1120, 1192, 1252, 1261, 1280
Alw21I GWGCWC 1 cut(s) 287
Alw26I GTCTC 2 cut(s) 263, 358
Alw44I GTGCAC 1 cut(s) 283
AlwI GGATC 5 cut(s) 76, 122, 135, 405, 634
Aor13HI TCCGGA 1 cut(s) 146
Aor51HI AGCGCT 1 cut(s) 299
AoxI GGCC 3 cut(s) 31, 441, 1164
ApaLI GTGCAC 1 cut(s) 283
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 3 cut(s) 391, 810, 1065
ArsI GACNNNNNNTTYG 2 cut(s) 1081, 1113
Asp700I GAANNNNTTC 1 cut(s) 315
AspLEI GCGC 4 cut(s) 41, 300, 426, 1039
AspS9I GGNCC 4 cut(s) 61, 88, 441, 1165
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 3 cut(s) 392, 787, 893
AsuII TTCGAA 1 cut(s) 414
AvaII GGWCC 2 cut(s) 61, 88
BaeGI GKGCMC 1 cut(s) 287
BamHI GGATCC 1 cut(s) 127
BanII GRGCYC 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 1247
Bbv12I GWGCWC 1 cut(s) 287
BbvI GCAGC 1 cut(s) 475
BccI CCATC 2 cut(s) 835, 904
BciT130I CCWGG 2 cut(s) 57, 181
BclI TGATCA 2 cut(s) 238, 733
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 2 cut(s) 263, 358
BfaI CTAG 1 cut(s) 704
BfmI CTRYAG 1 cut(s) 1302
BfoI RGCGCY 1 cut(s) 301
BisI GCNGC 5 cut(s) 42, 326, 444, 489, 507
BlpI GCTNAGC 1 cut(s) 382
BlsI GCNGC 5 cut(s) 43, 327, 445, 490, 508
Bme1390I CCNGG 3 cut(s) 57, 86, 181
Bme18I GGWCC 2 cut(s) 61, 88
BmgT120I GGNCC 4 cut(s) 61, 88, 441, 1165
BmiI GGNNCC 2 cut(s) 62, 129
BmrFI CCNGG 3 cut(s) 57, 86, 181
BmsI GCATC 5 cut(s) 180, 500, 576, 644, 1024
BpiI GAAGAC 1 cut(s) 1247
Bpu1102I GCTNAGC 1 cut(s) 382
Bpu14I TTCGAA 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 647
BpuMI CCSGG 1 cut(s) 86
BsaI GGTCTC 1 cut(s) 358
BsaJI CCNNGG 3 cut(s) 56, 403, 828
BsaWI WCCGGW 2 cut(s) 130, 146
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 70, 85
Bse118I RCCGGY 2 cut(s) 29, 878
Bse1I ACTGG 1 cut(s) 1049
Bse3DI GCAATG 1 cut(s) 643
BseAI TCCGGA 1 cut(s) 146
BseBI CCWGG 2 cut(s) 57, 181
BseDI CCNNGG 3 cut(s) 56, 403, 828
BseGI GGATG 2 cut(s) 143, 591
BseLI CCNNNNNNNGG 3 cut(s) 58, 70, 85
BseMI GCAATG 1 cut(s) 643
BseMII CTCAG 4 cut(s) 274, 396, 438, 1083
BseNI ACTGG 1 cut(s) 1049
BseRI GAGGAG 1 cut(s) 37
BseSI GKGCMC 1 cut(s) 287
BseXI GCAGC 1 cut(s) 475
Bsh1236I CGCG 4 cut(s) 39, 44, 328, 821
BshFI GGCC 3 cut(s) 33, 443, 1166
BsiHKAI GWGCWC 1 cut(s) 287
BsiSI CCGG 6 cut(s) 30, 86, 131, 147, 593, 879
BsiWI CGTACG 1 cut(s) 1026
BslFI GGGAC 2 cut(s) 74, 1235
BslI CCNNNNNNNGG 3 cut(s) 58, 70, 85
BsmAI GTCTC 2 cut(s) 263, 358
BsmFI GGGAC 2 cut(s) 74, 1235
BsmI GAATGC 2 cut(s) 1096, 1295
BsnI GGCC 3 cut(s) 33, 443, 1166
Bso31I GGTCTC 1 cut(s) 358
Bsp119I TTCGAA 1 cut(s) 414
Bsp1286I GDGCHC 2 cut(s) 287, 383
Bsp13I TCCGGA 1 cut(s) 146
Bsp143I GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
Bsp1720I GCTNAGC 1 cut(s) 382
BspANI GGCC 3 cut(s) 33, 443, 1166
BspCNI CTCAG 4 cut(s) 273, 395, 439, 1084
BspEI TCCGGA 1 cut(s) 146
BspFNI CGCG 4 cut(s) 39, 44, 328, 821
BspLI GGNNCC 2 cut(s) 62, 129
BspPI GGATC 5 cut(s) 76, 122, 135, 405, 634
BspT104I TTCGAA 1 cut(s) 414
BspTNI GGTCTC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 643
BsrFI RCCGGY 2 cut(s) 29, 878
BsrI ACTGG 1 cut(s) 1049
BssAI RCCGGY 2 cut(s) 29, 878
BssECI CCNNGG 3 cut(s) 56, 403, 828
BssMI GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
BssT1I CCWWGG 2 cut(s) 403, 828
Bst2UI CCWGG 2 cut(s) 57, 181
Bst4CI ACNGT 1 cut(s) 992
BstBI TTCGAA 1 cut(s) 414
BstC8I GCNNGC 5 cut(s) 379, 526, 819, 1135, 1139
BstDEI CTNAG 4 cut(s) 260, 382, 447, 1092
BstF5I GGATG 2 cut(s) 143, 591
BstFNI CGCG 4 cut(s) 39, 44, 328, 821
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 4 cut(s) 41, 300, 426, 1039
BstKTI GATC 9 cut(s) 84, 130, 241, 400, 629, 679, 736, 891, 1228
BstMAI GTCTC 2 cut(s) 263, 358
BstMBI GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
BstMWI GCNNNNNNNGC 4 cut(s) 383, 463, 937, 1258
BstNI CCWGG 2 cut(s) 57, 181
BstNSI RCATGY 1 cut(s) 749
BstSCI CCNGG 3 cut(s) 55, 84, 179
BstSFI CTRYAG 1 cut(s) 1302
BstSLI GKGCMC 1 cut(s) 287
BstUI CGCG 4 cut(s) 39, 44, 328, 821
BstV1I GCAGC 1 cut(s) 475
BstV2I GAAGAC 1 cut(s) 1247
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
BsuRI GGCC 3 cut(s) 33, 443, 1166
BtgZI GCGATG 2 cut(s) 744, 1020
BtsCI GGATG 2 cut(s) 143, 591
Cac8I GCNNGC 5 cut(s) 379, 526, 819, 1135, 1139
CfoI GCGC 4 cut(s) 41, 300, 426, 1039
Cfr10I RCCGGY 2 cut(s) 29, 878
Cfr13I GGNCC 4 cut(s) 61, 88, 441, 1165
Csp6I GTAC 3 cut(s) 497, 1027, 1179
CviAII CATG 3 cut(s) 70, 649, 746
CviQI GTAC 3 cut(s) 497, 1027, 1179
DdeI CTNAG 4 cut(s) 260, 382, 447, 1092
DpnI GATC 9 cut(s) 83, 129, 240, 399, 628, 678, 735, 890, 1227
DpnII GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
EaeI YGGCCR 1 cut(s) 31
EciI GGCGGA 2 cut(s) 776, 865
Eco130I CCWWGG 2 cut(s) 403, 828
Eco24I GRGCYC 1 cut(s) 383
Eco31I GGTCTC 1 cut(s) 358
Eco32I GATATC 2 cut(s) 108, 1077
Eco47I GGWCC 2 cut(s) 61, 88
Eco47III AGCGCT 1 cut(s) 299
Eco57I CTGAAG 2 cut(s) 771, 1277
EcoO109I RGGNCCY 2 cut(s) 61, 1165
EcoRII CCWGG 2 cut(s) 55, 179
EcoRV GATATC 2 cut(s) 108, 1077
EcoT14I CCWWGG 2 cut(s) 403, 828
EcoT22I ATGCAT 1 cut(s) 1295
EcoT38I GRGCYC 1 cut(s) 383
ErhI CCWWGG 2 cut(s) 403, 828
FaeI CATG 3 cut(s) 73, 652, 749
FaiI YATR 7 cut(s) 71, 255, 341, 473, 650, 747, 1046
FaqI GGGAC 2 cut(s) 74, 1235
FatI CATG 3 cut(s) 69, 648, 745
FauI CCCGC 4 cut(s) 185, 370, 826, 1211
FbaI TGATCA 2 cut(s) 238, 733
Fnu4HI GCNGC 5 cut(s) 42, 326, 444, 489, 507
FokI GGATG 2 cut(s) 130, 598
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 5 cut(s) 42, 326, 444, 489, 507
FspBI CTAG 1 cut(s) 704
FspI TGCGCA 1 cut(s) 425
GlaI GCGC 4 cut(s) 40, 299, 425, 1038
GluI GCNGC 5 cut(s) 42, 326, 444, 489, 507
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 3 cut(s) 33, 443, 1166
HapII CCGG 6 cut(s) 30, 86, 131, 147, 593, 879
HhaI GCGC 4 cut(s) 41, 300, 426, 1039
Hin1II CATG 3 cut(s) 73, 652, 749
Hin6I GCGC 4 cut(s) 39, 298, 424, 1037
HinP1I GCGC 4 cut(s) 39, 298, 424, 1037
HindIII AAGCTT 1 cut(s) 232
HinfI GANTC 9 cut(s) 244, 271, 361, 429, 515, 608, 947, 1267, 1298
HpaII CCGG 6 cut(s) 30, 86, 131, 147, 593, 879
HphI GGTGA 3 cut(s) 392, 787, 893
Hpy166II GTNNAC 2 cut(s) 285, 946
Hpy188I TCNGA 9 cut(s) 243, 263, 358, 803, 840, 952, 1081, 1188, 1257
Hpy188III TCNNGA 5 cut(s) 147, 275, 433, 1264, 1312
Hpy8I GTNNAC 2 cut(s) 285, 946
HpyAV CCTTC 8 cut(s) 118, 182, 625, 710, 1004, 1178, 1228, 1317
HpyCH4III ACNGT 1 cut(s) 992
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 9 cut(s) 285, 471, 491, 524, 548, 643, 648, 1133, 1293
HpyF10VI GCNNNNNNNGC 4 cut(s) 383, 463, 937, 1258
HpyF3I CTNAG 4 cut(s) 260, 382, 447, 1092
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 3 cut(s) 73, 652, 749
HspAI GCGC 4 cut(s) 39, 298, 424, 1037
Kpn2I TCCGGA 1 cut(s) 146
Ksp22I TGATCA 2 cut(s) 238, 733
Kzo9I GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
Lsp1109I GCAGC 1 cut(s) 475
LweI GCATC 5 cut(s) 180, 500, 576, 644, 1024
MaeI CTAG 1 cut(s) 704
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 2 cut(s) 133, 211
MalI GATC 9 cut(s) 83, 129, 240, 399, 628, 678, 735, 890, 1227
MboI GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
MboII GAAGA 8 cut(s) 572, 617, 861, 864, 898, 1072, 1157, 1252
MfeI CAATTG 1 cut(s) 549
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 2 cut(s) 287, 383
MluCI AATT 7 cut(s) 306, 391, 436, 549, 563, 810, 1065
MlyI GAGTC 2 cut(s) 941, 1307
MmeI TCCRAC 2 cut(s) 344, 563
MnlI CCTC 7 cut(s) 15, 74, 343, 963, 1008, 1065, 1156
Mph1103I ATGCAT 1 cut(s) 1295
MroI TCCGGA 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 6 cut(s) 30, 86, 131, 147, 593, 879
MspR9I CCNGG 3 cut(s) 57, 86, 181
MunI CAATTG 1 cut(s) 549
Mva1269I GAATGC 2 cut(s) 1096, 1295
MvaI CCWGG 2 cut(s) 57, 181
MvnI CGCG 4 cut(s) 39, 44, 328, 821
MwoI GCNNNNNNNGC 4 cut(s) 383, 463, 937, 1258
NciI CCSGG 1 cut(s) 86
NdeII GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
NlaIII CATG 3 cut(s) 73, 652, 749
NlaIV GGNNCC 2 cut(s) 62, 129
NmuCI GTSAC 2 cut(s) 133, 211
NsbI TGCGCA 1 cut(s) 425
NsiI ATGCAT 1 cut(s) 1295
NspI RCATGY 1 cut(s) 749
NspV TTCGAA 1 cut(s) 414
PcsI WCGNNNNNNNCGW 3 cut(s) 354, 635, 1106
PctI GAATGC 2 cut(s) 1096, 1295
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 7 cut(s) 244, 271, 361, 429, 515, 608, 1267
Pfl23II CGTACG 1 cut(s) 1026
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 5 cut(s) 43, 327, 445, 490, 508
PleI GAGTC 2 cut(s) 941, 1306
PpsI GAGTC 2 cut(s) 941, 1306
PpuMI RGGWCCY 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 61
Psp6I CCWGG 2 cut(s) 55, 179
PspGI CCWGG 2 cut(s) 55, 179
PspLI CGTACG 1 cut(s) 1026
PspN4I GGNNCC 2 cut(s) 62, 129
PspPI GGNCC 4 cut(s) 61, 88, 441, 1165
PspPPI RGGWCCY 1 cut(s) 61
PsuI RGATCY 1 cut(s) 127
RsaI GTAC 3 cut(s) 498, 1028, 1180
RsaNI GTAC 3 cut(s) 497, 1027, 1179
SatI GCNGC 5 cut(s) 42, 326, 444, 489, 507
Sau3AI GATC 9 cut(s) 81, 127, 238, 397, 626, 676, 733, 888, 1225
Sau96I GGNCC 4 cut(s) 61, 88, 441, 1165
SchI GAGTC 2 cut(s) 941, 1307
ScrFI CCNGG 3 cut(s) 57, 86, 181
SduI GDGCHC 2 cut(s) 287, 383
SfaNI GCATC 5 cut(s) 180, 500, 576, 644, 1024
SfcI CTRYAG 1 cut(s) 1302
SfuI TTCGAA 1 cut(s) 414
SgrAI CRCCGGYG 1 cut(s) 878
SinI GGWCC 2 cut(s) 61, 88
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
Sse9I AATT 7 cut(s) 306, 391, 436, 549, 563, 810, 1065
SspMI CTAG 1 cut(s) 704
StyD4I CCNGG 3 cut(s) 55, 84, 179
StyI CCWWGG 2 cut(s) 403, 828
TaaI ACNGT 1 cut(s) 992
TaiI ACGT 1 cut(s) 316
TaqI TCGA 9 cut(s) 66, 174, 186, 388, 414, 600, 629, 987, 1100
TaqII GACCGA 1 cut(s) 673
TasI AATT 7 cut(s) 306, 391, 436, 549, 563, 810, 1065
TatI WGTACW 1 cut(s) 496
TauI GCSGC 4 cut(s) 44, 328, 446, 509
TfiI GAWTC 7 cut(s) 244, 271, 361, 429, 515, 608, 1267
TseFI GTSAC 2 cut(s) 133, 211
TseI GCWGC 1 cut(s) 488
Tsp45I GTSAC 2 cut(s) 133, 211
TspDTI ATGAA 4 cut(s) 137, 618, 852, 1023
TspGWI ACGGA 1 cut(s) 318
VneI GTGCAC 1 cut(s) 283
VpaK11BI GGWCC 2 cut(s) 61, 88
XapI RAATTY 3 cut(s) 391, 810, 1065
XceI RCATGY 1 cut(s) 749
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 1 cut(s) 704
Zsp2I ATGCAT 1 cut(s) 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.