RLG00000005493

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001443
Physical Location & Seq
Reverse (-)
68081616 .. 68082968
1353 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005493

Sequence Viewer

Length: 1353 bp
ATGGTTTTGGGTTGGAGAAGAAGGAGCCGCACCGGCCACCGTGCCGCCGCTGCGAAAAAGCAGCCTTCACCTGGCAACCTCGACATGGATCAACTCGTGATCCCAAAGCATTTCCTCTGCCCAATATCTCTCGACTTGATGAAGGATCCGGTCACATTGTCCTCAGGTATAACTTATGATCGGCAAAGCATTGACACTTGGCTCGAAGCCGGCAACTTCAAGTGCCCGGTGACCAATCAGGTGCTGAGGAACTTTGATATGATTCCCAACCACACTCTCCGAACAATGATTCAAGATTGGAGCGCTCAAAACCAACGCTACGGCATTCAGCGTGTCCCAACTCCTAGAATTCCCATCATGGCAGTTGAGGTCTCCGAGATTCTGTTTAGCATCACAGCCTCAGTGAGACGCGTCGATCAAAATGGTTGCTTAGAGTGCGTGCAGATAATCAAGAAATGGGCGGCTGAGAGTGACCGAAACAAGCGCTGCATCGTGGAGAATGGAGCGGCCTCTGTTCTTGCTTCTGCTTTTGATGCTTTTGCAAATGATGGAATAAAGAAAAATGCTGGAGTGTGTGAAGAAATTTTGTCAGCTCTGAGTTGGATGATGTTTCCATCATTAGATGAAGAAACCCAAAAGTACTTGGGATCTCATGCTTCTTTGGATTGCCTGGTTTGGTTCCTCAAGGTTGATAGAGATCTTTTGGTGAAGCAAAACTCAATTCTAGCATTGAAAGAGCTTCTTTCTAATAGCAATGACCAGAACAAGCATGCGGTTGAAGCATTGGCAGAGATTGAAGGAGTCAATCAGATACTTTTCGGCTTCATCAAGGAGAAAAGTTCCGTGACGATTACCAAGTCTTCGTTAACAGTAGTCTTCTACTTGGTTTCATTCAGTGAGAAGTTGAGATCAACATTCTTGGAGATGGGTTTGGTTTCCCTATTGTTAGAGACCATTCTCGACTCGGAAAAAGGCATAATGGAGAGGGCTTTGTCTGTTTTGGATTGTCTTTGTGATTGCAAAGAAGGGAGGGAACAGGCCTATGCTAATGCGCTGACTATACCAGTTTTGGTGAAGAAGATTTTTAGGGTGTCAGAAATGGCGACTGAGTATTCGATCTCTGCTGTTTGGAAGCTGTGTAAGAATGCAACTAGGCAAGAAGAGAGAGTACTTGTTGAGGCTCTTCAAGTTGGTGCATTTCAGAAGCTCTTGATGGTTTTACAGGTTCGTTGCGCCGGTGAATATGACACAAAGGACAAACTAACTGAGCTTTTGAAGCTGTTGAATCCTTACAGAGCTGGATTGGAATGCATCGAGTCTGTAGATTTCAAGAGTATTAAAAGGTCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010033 GO:0010200 GO:0010243 GO:0042221 GO:0042493 GO:0050896 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

451

Amino Acids

50.62

Weight (kDa)

7.87

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 34 - 105 1.5e-21 U-box domain
ARM_PUB PF25598 123 - 445 2.9e-61 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 506
AccII CGCG 1 cut(s) 411
AciI CCGC 6 cut(s) 28, 45, 48, 461, 506, 773
AclWI GGATC 5 cut(s) 94, 96, 140, 153, 655
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 2 cut(s) 348, 582
AfaI GTAC 2 cut(s) 641, 1170
AfeI AGCGCT 2 cut(s) 304, 485
AfiI CCNNNNNNNGG 2 cut(s) 71, 85
AflIII ACRYGT 1 cut(s) 409
AgsI TTSAA 9 cut(s) 220, 293, 733, 779, 797, 1187, 1276, 1285, 1330
AjnI CCWGG 2 cut(s) 70, 669
AluBI AGCT 7 cut(s) 593, 739, 1135, 1207, 1270, 1279, 1298
AluI AGCT 7 cut(s) 593, 739, 1135, 1207, 1270, 1279, 1298
Alw26I GTCTC 3 cut(s) 376, 400, 944
AlwI GGATC 5 cut(s) 94, 96, 140, 153, 655
AlwNI CAGNNNCTG 1 cut(s) 244
Aor51HI AGCGCT 2 cut(s) 304, 485
AoxI GGCC 3 cut(s) 34, 507, 1038
ApeKI GCWGC 3 cut(s) 50, 61, 486
ApoI RAATTY 2 cut(s) 348, 582
ArsI GACNNNNNNTTYG 2 cut(s) 1096, 1128
AspLEI GCGC 4 cut(s) 305, 486, 1054, 1235
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 5 cut(s) 60, 241, 718, 1084, 1250
AxyI CCTNAGG 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 227
BamHI GGATCC 1 cut(s) 145
BarI GAAGNNNNNNTAC 2 cut(s) 1152, 1184
BauI CACGAG 1 cut(s) 95
BbsI GAAGAC 2 cut(s) 852, 868
BbvCI CCTCAGC 1 cut(s) 245
BbvI GCAGC 3 cut(s) 37, 73, 473
BccI CCATC 5 cut(s) 362, 542, 622, 919, 1207
BceAI ACGGC 1 cut(s) 337
BciT130I CCWGG 2 cut(s) 72, 671
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 3 cut(s) 376, 400, 944
BfaI CTAG 3 cut(s) 345, 725, 1152
BfmI CTRYAG 1 cut(s) 1320
BfoI RGCGCY 2 cut(s) 306, 487
BglI GCCNNNNNGGC 1 cut(s) 33
BglII AGATCT 1 cut(s) 697
BisI GCNGC 8 cut(s) 28, 45, 48, 51, 62, 462, 487, 507
BlsI GCNGC 8 cut(s) 29, 46, 49, 52, 63, 463, 488, 508
BmcAI AGTACT 2 cut(s) 641, 1170
Bme1390I CCNGG 3 cut(s) 72, 227, 671
BmiI GGNNCC 3 cut(s) 26, 147, 680
BmrFI CCNGG 3 cut(s) 72, 227, 671
BmsI GCATC 4 cut(s) 399, 498, 523, 1320
BpiI GAAGAC 2 cut(s) 852, 868
BpmI CTGGAG 1 cut(s) 588
Bpu10I CCTNAGC 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 696
BsaI GGTCTC 2 cut(s) 376, 944
BsaWI WCCGGW 1 cut(s) 148
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 85
Bse118I RCCGGY 3 cut(s) 32, 209, 1235
Bse1I ACTGG 1 cut(s) 1064
Bse21I CCTNAGG 1 cut(s) 163
Bse3DI GCAATG 1 cut(s) 760
Bse8I GATNNNNATC 1 cut(s) 696
BseBI CCWGG 2 cut(s) 72, 671
BseGI GGATG 1 cut(s) 609
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 2 cut(s) 71, 85
BseMI GCAATG 1 cut(s) 760
BseMII CTCAG 7 cut(s) 177, 236, 414, 456, 587, 1098, 1257
BseNI ACTGG 1 cut(s) 1064
BseSI GKGCMC 1 cut(s) 227
BseXI GCAGC 3 cut(s) 37, 73, 473
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 1 cut(s) 411
BshFI GGCC 3 cut(s) 36, 509, 1040
BsiSI CCGG 5 cut(s) 33, 149, 210, 227, 1236
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 2 cut(s) 71, 85
BsmAI GTCTC 3 cut(s) 376, 400, 944
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 1 cut(s) 320
BsmI GAATGC 3 cut(s) 324, 1150, 1313
BsnI GGCC 3 cut(s) 36, 509, 1040
Bso31I GGTCTC 2 cut(s) 376, 944
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 9 cut(s) 88, 99, 145, 178, 415, 647, 697, 908, 1116
BspACI CCGC 6 cut(s) 28, 45, 48, 461, 506, 773
BspANI GGCC 3 cut(s) 36, 509, 1040
BspCNI CTCAG 7 cut(s) 176, 237, 413, 457, 588, 1099, 1258
BspFNI CGCG 1 cut(s) 411
BspLI GGNNCC 3 cut(s) 26, 147, 680
BspPI GGATC 5 cut(s) 94, 96, 140, 153, 655
BspQI GCTCTTC 1 cut(s) 1188
BspTNI GGTCTC 2 cut(s) 376, 944
BsrBI CCGCTC 1 cut(s) 506
BsrDI GCAATG 1 cut(s) 760
BsrFI RCCGGY 3 cut(s) 32, 209, 1235
BsrI ACTGG 1 cut(s) 1064
BssAI RCCGGY 3 cut(s) 32, 209, 1235
BssMI GATC 9 cut(s) 88, 99, 145, 178, 415, 647, 697, 908, 1116
BssSI CACGAG 1 cut(s) 95
Bst2BI CACGAG 1 cut(s) 95
Bst2UI CCWGG 2 cut(s) 72, 671
Bst4CI ACNGT 2 cut(s) 41, 871
Bst6I CTCTTC 2 cut(s) 1155, 1188
BstC8I GCNNGC 3 cut(s) 211, 440, 771
BstDEI CTNAG 8 cut(s) 163, 245, 400, 430, 465, 596, 1107, 1266
BstEII GGTNACC 1 cut(s) 229
BstF5I GGATG 1 cut(s) 609
BstFNI CGCG 1 cut(s) 411
BstH2I RGCGCY 2 cut(s) 306, 487
BstHHI GCGC 4 cut(s) 305, 486, 1054, 1235
BstKTI GATC 9 cut(s) 91, 102, 148, 181, 418, 650, 700, 911, 1119
BstMAI GTCTC 3 cut(s) 376, 400, 944
BstMBI GATC 9 cut(s) 88, 99, 145, 178, 415, 647, 697, 908, 1116
BstMWI GCNNNNNNNGC 6 cut(s) 33, 50, 435, 533, 779, 1276
BstNI CCWGG 2 cut(s) 72, 671
BstNSI RCATGY 1 cut(s) 773
BstPI GGTNACC 1 cut(s) 229
BstSCI CCNGG 3 cut(s) 70, 225, 669
BstSFI CTRYAG 1 cut(s) 1320
BstSLI GKGCMC 1 cut(s) 227
BstUI CGCG 1 cut(s) 411
BstV1I GCAGC 3 cut(s) 37, 73, 473
BstV2I GAAGAC 2 cut(s) 852, 868
BstX2I RGATCY 3 cut(s) 145, 647, 697
BstYI RGATCY 3 cut(s) 145, 647, 697
Bsu36I CCTNAGG 1 cut(s) 163
BsuRI GGCC 3 cut(s) 36, 509, 1040
BtsCI GGATG 1 cut(s) 609
BtsIMutI CAGTG 2 cut(s) 408, 901
Cac8I GCNNGC 3 cut(s) 211, 440, 771
CaiI CAGNNNCTG 1 cut(s) 244
CfoI GCGC 4 cut(s) 305, 486, 1054, 1235
Cfr10I RCCGGY 3 cut(s) 32, 209, 1235
CseI GACGC 2 cut(s) 400, 417
Csp6I GTAC 2 cut(s) 640, 1169
CviAII CATG 4 cut(s) 85, 358, 653, 770
CviQI GTAC 2 cut(s) 640, 1169
DdeI CTNAG 8 cut(s) 163, 245, 400, 430, 465, 596, 1107, 1266
DpnI GATC 9 cut(s) 90, 101, 147, 180, 417, 649, 699, 910, 1118
DpnII GATC 9 cut(s) 88, 99, 145, 178, 415, 647, 697, 908, 1116
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 1155, 1188
EarI CTCTTC 2 cut(s) 1155, 1188
Eco147I AGGCCT 1 cut(s) 1040
Eco31I GGTCTC 2 cut(s) 376, 944
Eco47III AGCGCT 2 cut(s) 304, 485
Eco81I CCTNAGG 1 cut(s) 163
Eco91I GGTNACC 1 cut(s) 229
EcoO65I GGTNACC 1 cut(s) 229
EcoRI GAATTC 1 cut(s) 348
EcoRII CCWGG 2 cut(s) 70, 669
EcoT22I ATGCAT 1 cut(s) 1313
Esp3I CGTCTC 1 cut(s) 400
FaeI CATG 4 cut(s) 88, 361, 656, 773
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 1 cut(s) 320
FatI CATG 4 cut(s) 84, 357, 652, 769
Fnu4HI GCNGC 8 cut(s) 28, 45, 48, 51, 62, 462, 487, 507
FokI GGATG 1 cut(s) 616
Fsp4HI GCNGC 8 cut(s) 28, 45, 48, 51, 62, 462, 487, 507
FspBI CTAG 3 cut(s) 345, 725, 1152
GlaI GCGC 4 cut(s) 304, 485, 1053, 1234
GluI GCNGC 8 cut(s) 28, 45, 48, 51, 62, 462, 487, 507
GsuI CTGGAG 1 cut(s) 588
HaeII RGCGCY 2 cut(s) 306, 487
HaeIII GGCC 3 cut(s) 36, 509, 1040
HapII CCGG 5 cut(s) 33, 149, 210, 227, 1236
HgaI GACGC 2 cut(s) 400, 417
HhaI GCGC 4 cut(s) 305, 486, 1054, 1235
Hin1II CATG 4 cut(s) 88, 361, 656, 773
Hin6I GCGC 4 cut(s) 303, 484, 1052, 1233
HinP1I GCGC 4 cut(s) 303, 484, 1052, 1233
HincII GTYRAC 1 cut(s) 867
HindII GTYRAC 1 cut(s) 867
HinfI GANTC 7 cut(s) 262, 289, 379, 801, 962, 1285, 1316
HpaI GTTAAC 1 cut(s) 867
HpaII CCGG 5 cut(s) 33, 149, 210, 227, 1236
HphI GGTGA 5 cut(s) 60, 241, 718, 1084, 1250
Hpy166II GTNNAC 1 cut(s) 867
Hpy188I TCNGA 8 cut(s) 281, 376, 597, 810, 967, 1096, 1203, 1352
Hpy188III TCNNGA 7 cut(s) 97, 131, 293, 451, 959, 1210, 1330
Hpy8I GTNNAC 1 cut(s) 867
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 5 cut(s) 15, 75, 136, 791, 1019
HpyCH4III ACNGT 2 cut(s) 41, 871
HpyCH4V TGCA 7 cut(s) 442, 489, 542, 1020, 1148, 1196, 1311
HpyF10VI GCNNNNNNNGC 6 cut(s) 33, 50, 435, 533, 779, 1276
HpyF3I CTNAG 8 cut(s) 163, 245, 400, 430, 465, 596, 1107, 1266
Hsp92II CATG 4 cut(s) 88, 361, 656, 773
HspAI GCGC 4 cut(s) 303, 484, 1052, 1233
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
KspAI GTTAAC 1 cut(s) 867
Kzo9I GATC 9 cut(s) 88, 99, 145, 178, 415, 647, 697, 908, 1116
LguI GCTCTTC 1 cut(s) 1188
LmnI GCTCC 3 cut(s) 24, 300, 503
Lsp1109I GCAGC 3 cut(s) 37, 73, 473
LweI GCATC 4 cut(s) 399, 498, 523, 1320
MaeI CTAG 3 cut(s) 345, 725, 1152
MaeIII GTNAC 4 cut(s) 151, 229, 470, 844
MalI GATC 9 cut(s) 90, 101, 147, 180, 417, 649, 699, 910, 1118
MbiI CCGCTC 1 cut(s) 506
MboI GATC 9 cut(s) 88, 99, 145, 178, 415, 647, 697, 908, 1116
MboII GAAGA 9 cut(s) 30, 590, 638, 852, 868, 1087, 1090, 1172, 1175
MflI RGATCY 3 cut(s) 145, 647, 697
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 3 cut(s) 348, 582, 720
MluI ACGCGT 1 cut(s) 409
MlyI GAGTC 3 cut(s) 810, 956, 1325
MmeI TCCRAC 1 cut(s) 581
Mph1103I ATGCAT 1 cut(s) 1313
MroNI GCCGGC 1 cut(s) 209
MseI TTAA 2 cut(s) 866, 1338
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 5 cut(s) 33, 149, 210, 227, 1236
MspR9I CCNGG 3 cut(s) 72, 227, 671
Mva1269I GAATGC 3 cut(s) 324, 1150, 1313
MvaI CCWGG 2 cut(s) 72, 671
MvnI CGCG 1 cut(s) 411
MwoI GCNNNNNNNGC 6 cut(s) 33, 50, 435, 533, 779, 1276
NaeI GCCGGC 1 cut(s) 211
NciI CCSGG 1 cut(s) 227
NdeII GATC 9 cut(s) 88, 99, 145, 178, 415, 647, 697, 908, 1116
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 4 cut(s) 88, 361, 656, 773
NlaIV GGNNCC 3 cut(s) 26, 147, 680
NmuCI GTSAC 4 cut(s) 151, 229, 470, 844
NsiI ATGCAT 1 cut(s) 1313
NspI RCATGY 1 cut(s) 773
PaeI GCATGC 1 cut(s) 773
PceI AGGCCT 1 cut(s) 1040
PciSI GCTCTTC 1 cut(s) 1188
PctI GAATGC 3 cut(s) 324, 1150, 1313
PdiI GCCGGC 1 cut(s) 211
PfeI GAWTC 4 cut(s) 262, 289, 379, 1285
PkrI GCNGC 8 cut(s) 29, 46, 49, 52, 63, 463, 488, 508
PleI GAGTC 3 cut(s) 809, 956, 1324
PpsI GAGTC 3 cut(s) 809, 956, 1324
Psp6I CCWGG 2 cut(s) 70, 669
PspEI GGTNACC 1 cut(s) 229
PspGI CCWGG 2 cut(s) 70, 669
PspN4I GGNNCC 3 cut(s) 26, 147, 680
PstNI CAGNNNCTG 1 cut(s) 244
PsuI RGATCY 3 cut(s) 145, 647, 697
RsaI GTAC 2 cut(s) 641, 1170
RsaNI GTAC 2 cut(s) 640, 1169
SapI GCTCTTC 1 cut(s) 1188
SaqAI TTAA 2 cut(s) 866, 1338
SatI GCNGC 8 cut(s) 28, 45, 48, 51, 62, 462, 487, 507
Sau3AI GATC 9 cut(s) 88, 99, 145, 178, 415, 647, 697, 908, 1116
ScaI AGTACT 2 cut(s) 641, 1170
SchI GAGTC 3 cut(s) 810, 956, 1325
ScrFI CCNGG 3 cut(s) 72, 227, 671
SduI GDGCHC 1 cut(s) 227
SfaNI GCATC 4 cut(s) 399, 498, 523, 1320
SfcI CTRYAG 1 cut(s) 1320
SgrAI CRCCGGYG 1 cut(s) 1235
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
SphI GCATGC 1 cut(s) 773
Sse9I AATT 3 cut(s) 348, 582, 720
SseBI AGGCCT 1 cut(s) 1040
SsiI CCGC 6 cut(s) 28, 45, 48, 461, 506, 773
SspMI CTAG 3 cut(s) 345, 725, 1152
StuI AGGCCT 1 cut(s) 1040
StyD4I CCNGG 3 cut(s) 70, 225, 669
TaaI ACNGT 2 cut(s) 41, 871
TaqI TCGA 7 cut(s) 81, 132, 204, 414, 960, 1115, 1314
TaqII GACCGA 1 cut(s) 489
TasI AATT 3 cut(s) 348, 582, 720
TatI WGTACW 2 cut(s) 639, 1168
TauI GCSGC 5 cut(s) 30, 47, 50, 464, 509
TfiI GAWTC 4 cut(s) 262, 289, 379, 1285
Tru1I TTAA 2 cut(s) 866, 1338
Tru9I TTAA 2 cut(s) 866, 1338
TscAI CASTG 2 cut(s) 408, 901
TseFI GTSAC 4 cut(s) 151, 229, 470, 844
TseI GCWGC 3 cut(s) 50, 61, 486
Tsp45I GTSAC 4 cut(s) 151, 229, 470, 844
TspDTI ATGAA 4 cut(s) 155, 639, 814, 879
TspGWI ACGGA 1 cut(s) 832
TspRI CASTG 2 cut(s) 408, 901
XapI RAATTY 2 cut(s) 348, 582
XceI RCATGY 1 cut(s) 773
XspI CTAG 3 cut(s) 345, 725, 1152
ZrmI AGTACT 2 cut(s) 641, 1170
Zsp2I ATGCAT 1 cut(s) 1313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.